Rorug02G0136900

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0016196
Physical Location & Seq
Forward (+)
12063152 .. 12065218
2067 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0136900.1

Sequence Viewer

Length: 1494 bp
ATGTTTCCACTCAATTTTGAGAGTCTCAATCTCACAAACATGCCCACAACGGCATCGTCCTTGTTCTCAGCCTATGCCTCCCTCACCGCATTCATAATGTTGGTCCGTTCCATAGTTGATCAGTTGATCCCCCACGAGCTCCGCTACTACATCTACTCGGCCTTCGGCCGCCTCTTCACCCCTCGCCCCTCCGACCTCACTCTCATCGTCGATGAGTGCTGCGGCATGGTTCAAAACCAAATCTATGAGGCAGCGGAGGTCTACCTCCGGACCAAGATCAGTCCCCTAAACGACCGTCTCCGGGTGAAAAAGGCACCTCGGCAGAAGAACATTACCGTCACGATTGAAAAGGGTGAAGAAATCAAAGACAGTTTCGACGGGATAAACATCAACTGGCGTTTTGTGTGCAGCGGAGGAGAGAATGAGCAGAACCGCCATGCGCAACAGAAGCGTCAGTTTGAGCTGACAGTGAATAAGAAACATCGAGTCAAAGTAATTGAGTCCTACTTGCCGTATGTGCTTGACAGGGCCGAGGCTATTAAGAATGAGGACAAGGTTGTGAAGCTCTATAGCCAAGAGGGGTCATATGATCCGTATGATGATGAATCCAATTCGGACTGGAGTTCGATAAATCTGGAGCATCCGGCTACCTTTGAGACACTTGCCATGGATGCCGAGGTTAAGAGAACGATCATTGAGGATCTGGATAGGTTTTTAAGGAGGAGGGAGTTTTATAAGAAGGTTGGGAAGGCCTGGAAAAGAGGGTATTTGTTGTATGGTCCACCCGGGACTGGCAAATCCAGCTTGATTGCTGCCATGGCTAATTATCTCAAGTTTGATATATATGATGTGGAGCTTACTAGTATTGAAGACAACTCGGAGTTGAGGAGGATACTGCTGACTACGACAAACCGTTCTATCTTGGTCATTGAGGATATTGATTGCAGTGTGGAGGTGCAAAACCGGGAATGTGAGGAGGAAGATCATCAACCATCTAACACCAAGTTAACACTCTCCGGCCTACTGAACTTTATAGATGGTTTGTGGTCAAGCTGCGGAGATGAGAGAATCATTGTGTTCACCACCAACCACAAGCACAAGATAGACCCGGCATTGTTGCGTCCTGGTCGAATGGATGTGCACATTCACATGTCATATTGCACTCCAAGCGGGTTCAGGATCTTGGCCTCTAACTACCTTGGTATGCATGAAAGCAATCCTCATCGCCTATGCGGAGAAGTTGAGGAGTTAATAGAGAGCTCAAAGGTCACCCCCGCGGAGATTGCTGAGCAGCTCATGAAGAGTGAGGATGCTGATGTTGCTCTTGAAGGACTTGTTGATTTCCTGAAAAGAAAGAAGGAAGAGAGCAATAAAATTGGGGATGAAGGGACTAATAAATCTGAAATTCAAGAAACAGATCAAAACGCAGAAAAGCGGAGCTCAGTCAGTAGCGATGATGAACTTGAATCTTCTCTTTCAGATGCTCAAGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

497

Amino Acids

56.76

Weight (kDa)

5.15

Isoelectric Point (pI)

56.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AAA_assoc PF14363 43 - 136 3.2e-25 Domain associated at C-terminal with AAA
AAA PF00004 256 - 385 1.8e-20 ATPase family associated with various cellular activities (AAA)
AAA_lid_At3g28540 PF25568 387 - 457 9.5e-22 At3g28540-like, AAA+ ATPase lid domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016196)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G67265
fragaria_vesca FvH4_1g16720
malus_domestica MD02G1178400.v1.1
prunus_persica Prupe.6G229400_v2.0.a1
rosa_laevigata RLG00000017524
rosa_multiflora Rmu_co8325987.1_g000001
rosa_rugosa Rorug02G0136900
rosa_samantha Rh2AG188200 Rh2AG188300 Rh2BG199200 Rh2BG199300 Rh2CG193300 Rh2DG194400 Rh2DG194500
rosa_wichuraiana Rw2G014780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 735
Acc16I TGCGCA 1 cut(s) 441
AccB1I GGYRCC 1 cut(s) 313
AccI GTMKAC 1 cut(s) 261
AccII CGCG 1 cut(s) 1277
AccIII TCCGGA 1 cut(s) 267
AclWI GGATC 4 cut(s) 121, 584, 708, 1187
AcoI YGGCCR 1 cut(s) 166
AcsI RAATTY 1 cut(s) 1404
AfiI CCNNNNNNNGG 2 cut(s) 301, 791
AflIII ACRYGT 1 cut(s) 1149
AgsI TTSAA 6 cut(s) 233, 347, 869, 1328, 1409, 1466
AhlI ACTAGT 1 cut(s) 860
AjnI CCWGG 2 cut(s) 752, 1123
AluBI AGCT 9 cut(s) 139, 463, 565, 804, 856, 1053, 1260, 1294, 1440
AluI AGCT 9 cut(s) 139, 463, 565, 804, 856, 1053, 1260, 1294, 1440
Alw21I GWGCWC 4 cut(s) 141, 1143, 1262, 1442
Alw26I GTCTC 3 cut(s) 29, 302, 650
Alw44I GTGCAC 1 cut(s) 1139
AlwI GGATC 4 cut(s) 121, 584, 708, 1187
Ama87I CYCGRG 1 cut(s) 785
Aor13HI TCCGGA 1 cut(s) 267
AoxI GGCC 6 cut(s) 159, 166, 528, 750, 1018, 1185
ApaLI GTGCAC 1 cut(s) 1139
ApeKI GCWGC 6 cut(s) 219, 251, 408, 812, 1053, 1291
ApoI RAATTY 1 cut(s) 1404
AspLEI GCGC 1 cut(s) 442
AspS9I GGNCC 4 cut(s) 103, 270, 528, 779
AsuC2I CCSGG 5 cut(s) 302, 786, 787, 965, 1109
AsuHPI GGTGA 6 cut(s) 76, 169, 316, 365, 1072, 1261
AvaI CYCGRG 1 cut(s) 785
AvaII GGWCC 3 cut(s) 103, 270, 779
BaeGI GKGCMC 1 cut(s) 1143
BanI GGYRCC 1 cut(s) 313
BanII GRGCYC 3 cut(s) 141, 1262, 1442
BauI CACGAG 1 cut(s) 134
BbsI GAAGAC 1 cut(s) 876
Bbv12I GWGCWC 4 cut(s) 141, 1143, 1262, 1442
BbvI GCAGC 6 cut(s) 206, 263, 420, 799, 1040, 1303
BccI CCATC 2 cut(s) 1000, 1031
BceAI ACGGC 2 cut(s) 66, 496
BciT130I CCWGG 2 cut(s) 754, 1125
BciVI GTATCC 1 cut(s) 885
BclI TGATCA 1 cut(s) 118
BcnI CCSGG 5 cut(s) 302, 786, 787, 965, 1109
BcoDI GTCTC 3 cut(s) 29, 302, 650
BcuI ACTAGT 1 cut(s) 860
BfaI CTAG 1 cut(s) 861
BfmI CTRYAG 1 cut(s) 568
BfuI GTATCC 1 cut(s) 885
BisI GCNGC 8 cut(s) 169, 220, 223, 252, 409, 813, 1054, 1292
BlpI GCTNAGC 1 cut(s) 1287
BlsI GCNGC 8 cut(s) 170, 221, 224, 253, 410, 814, 1055, 1293
Bme1390I CCNGG 7 cut(s) 302, 754, 786, 787, 965, 1109, 1125
Bme18I GGWCC 3 cut(s) 103, 270, 779
BmeT110I CYCGRG 1 cut(s) 785
BmgT120I GGNCC 4 cut(s) 103, 270, 528, 779
BmiI GGNNCC 1 cut(s) 315
BmrFI CCNGG 7 cut(s) 302, 754, 786, 787, 965, 1109, 1125
BmsI GCATC 5 cut(s) 62, 649, 661, 1300, 1471
BpiI GAAGAC 1 cut(s) 876
BpmI CTGGAG 2 cut(s) 640, 656
Bpu1102I GCTNAGC 1 cut(s) 1287
BpuEI CTTGAG 2 cut(s) 815, 1470
BpuMI CCSGG 5 cut(s) 302, 786, 787, 965, 1109
BsaBI GATNNNNATC 1 cut(s) 386
BsaJI CCNNGG 8 cut(s) 317, 531, 666, 675, 785, 816, 1198, 1275
BsaWI WCCGGW 1 cut(s) 267
Bsc4I CCNNNNNNNGG 2 cut(s) 301, 791
Bse1I ACTGG 3 cut(s) 398, 623, 796
Bse8I GATNNNNATC 1 cut(s) 386
BseAI TCCGGA 1 cut(s) 267
BseBI CCWGG 2 cut(s) 754, 1125
BseDI CCNNGG 8 cut(s) 317, 531, 666, 675, 785, 816, 1198, 1275
BseGI GGATG 5 cut(s) 640, 676, 1141, 1315, 1387
BseJI GATNNNNATC 1 cut(s) 386
BseLI CCNNNNNNNGG 2 cut(s) 301, 791
BseMII CTCAG 3 cut(s) 81, 1278, 1455
BseNI ACTGG 3 cut(s) 398, 623, 796
BseRI GAGGAG 5 cut(s) 429, 736, 901, 989, 1259
BseSI GKGCMC 1 cut(s) 1143
BseX3I CGGCCG 1 cut(s) 166
BseXI GCAGC 6 cut(s) 206, 263, 420, 799, 1040, 1303
BsgI GTGCAG 1 cut(s) 427
Bsh1236I CGCG 1 cut(s) 1277
Bsh1285I CGRYCG 2 cut(s) 169, 295
BshFI GGCC 6 cut(s) 161, 168, 530, 752, 1020, 1187
BshNI GGYRCC 1 cut(s) 313
BsiEI CGRYCG 2 cut(s) 169, 295
BsiHKAI GWGCWC 4 cut(s) 141, 1143, 1262, 1442
BsiHKCI CYCGRG 1 cut(s) 785
BsiSI CCGG 7 cut(s) 268, 301, 644, 786, 964, 1017, 1109
BslFI GGGAC 3 cut(s) 267, 802, 1402
BslI CCNNNNNNNGG 2 cut(s) 301, 791
BsmAI GTCTC 3 cut(s) 29, 302, 650
BsmBI CGTCTC 1 cut(s) 302
BsmFI GGGAC 3 cut(s) 267, 802, 1402
BsmI GAATGC 1 cut(s) 89
BsnI GGCC 6 cut(s) 161, 168, 530, 752, 1020, 1187
BsoBI CYCGRG 1 cut(s) 785
Bsp1286I GDGCHC 4 cut(s) 141, 1143, 1262, 1442
Bsp13I TCCGGA 1 cut(s) 267
Bsp143I GATC 9 cut(s) 118, 126, 276, 589, 690, 700, 982, 1179, 1417
Bsp1720I GCTNAGC 1 cut(s) 1287
Bsp19I CCATGG 2 cut(s) 666, 816
BspANI GGCC 6 cut(s) 161, 168, 530, 752, 1020, 1187
BspCNI CTCAG 3 cut(s) 80, 1279, 1454
BspEI TCCGGA 1 cut(s) 267
BspFNI CGCG 1 cut(s) 1277
BspHI TCATGA 1 cut(s) 1296
BspLI GGNNCC 1 cut(s) 315
BspPI GGATC 4 cut(s) 121, 584, 708, 1187
BspT107I GGYRCC 1 cut(s) 313
BsrI ACTGG 3 cut(s) 398, 623, 796
BssECI CCNNGG 8 cut(s) 317, 531, 666, 675, 785, 816, 1198, 1275
BssMI GATC 9 cut(s) 118, 126, 276, 589, 690, 700, 982, 1179, 1417
BssSI CACGAG 1 cut(s) 134
BssT1I CCWWGG 3 cut(s) 666, 816, 1198
Bst2BI CACGAG 1 cut(s) 134
Bst2UI CCWGG 2 cut(s) 754, 1125
Bst4CI ACNGT 5 cut(s) 296, 337, 371, 469, 914
Bst6I CTCTTC 3 cut(s) 179, 1295, 1356
BstDEI CTNAG 3 cut(s) 67, 1287, 1441
BstDSI CCRYGG 3 cut(s) 666, 816, 1275
BstEII GGTNACC 1 cut(s) 1267
BstF5I GGATG 5 cut(s) 640, 676, 1141, 1315, 1387
BstFNI CGCG 1 cut(s) 1277
BstHHI GCGC 1 cut(s) 442
BstKTI GATC 9 cut(s) 121, 129, 279, 592, 693, 703, 985, 1182, 1420
BstMAI GTCTC 3 cut(s) 29, 302, 650
BstMBI GATC 9 cut(s) 118, 126, 276, 589, 690, 700, 982, 1179, 1417
BstMCI CGRYCG 2 cut(s) 169, 295
BstMWI GCNNNNNNNGC 8 cut(s) 448, 517, 671, 801, 818, 1167, 1283, 1319
BstNI CCWGG 2 cut(s) 754, 1125
BstNSI RCATGY 2 cut(s) 43, 1153
BstPI GGTNACC 1 cut(s) 1267
BstSCI CCNGG 7 cut(s) 300, 752, 784, 785, 963, 1107, 1123
BstSFI CTRYAG 1 cut(s) 568
BstSLI GKGCMC 1 cut(s) 1143
BstUI CGCG 1 cut(s) 1277
BstV1I GCAGC 6 cut(s) 206, 263, 420, 799, 1040, 1303
BstV2I GAAGAC 1 cut(s) 876
BstX2I RGATCY 2 cut(s) 700, 1179
BstYI RGATCY 2 cut(s) 700, 1179
BstZI CGGCCG 1 cut(s) 166
BsuI GTATCC 1 cut(s) 885
BsuRI GGCC 6 cut(s) 161, 168, 530, 752, 1020, 1187
BtgI CCRYGG 3 cut(s) 666, 816, 1275
BtgZI GCGATG 2 cut(s) 1208, 1467
BtsCI GGATG 5 cut(s) 640, 676, 1141, 1315, 1387
BtsI GCAGTG 1 cut(s) 952
BtsIMutI CAGTG 2 cut(s) 474, 952
CciI TCATGA 1 cut(s) 1296
CfoI GCGC 1 cut(s) 442
Cfr13I GGNCC 4 cut(s) 103, 270, 528, 779
Cfr42I CCGCGG 1 cut(s) 1278
Cfr9I CCCGGG 1 cut(s) 785
CseI GACGC 2 cut(s) 440, 1109
CviAII CATG 8 cut(s) 40, 226, 437, 667, 817, 1150, 1208, 1297
DdeI CTNAG 3 cut(s) 67, 1287, 1441
DpnI GATC 9 cut(s) 120, 128, 278, 591, 692, 702, 984, 1181, 1419
DpnII GATC 9 cut(s) 118, 126, 276, 589, 690, 700, 982, 1179, 1417
EaeI YGGCCR 1 cut(s) 166
EagI CGGCCG 1 cut(s) 166
Eam1104I CTCTTC 3 cut(s) 179, 1295, 1356
EarI CTCTTC 3 cut(s) 179, 1295, 1356
Ecl136II GAGCTC 3 cut(s) 139, 1260, 1440
EclXI CGGCCG 1 cut(s) 166
Eco130I CCWWGG 3 cut(s) 666, 816, 1198
Eco147I AGGCCT 1 cut(s) 752
Eco24I GRGCYC 3 cut(s) 141, 1262, 1442
Eco47I GGWCC 3 cut(s) 103, 270, 779
Eco52I CGGCCG 1 cut(s) 166
Eco53kI GAGCTC 3 cut(s) 139, 1260, 1440
Eco88I CYCGRG 1 cut(s) 785
Eco91I GGTNACC 1 cut(s) 1267
EcoICRI GAGCTC 3 cut(s) 139, 1260, 1440
EcoO65I GGTNACC 1 cut(s) 1267
EcoRII CCWGG 2 cut(s) 752, 1123
EcoT14I CCWWGG 3 cut(s) 666, 816, 1198
EcoT22I ATGCAT 1 cut(s) 1209
EcoT38I GRGCYC 3 cut(s) 141, 1262, 1442
ErhI CCWWGG 3 cut(s) 666, 816, 1198
Esp3I CGTCTC 1 cut(s) 302
FaeI CATG 8 cut(s) 43, 229, 440, 670, 820, 1153, 1211, 1300
FaqI GGGAC 3 cut(s) 267, 802, 1402
FatI CATG 8 cut(s) 39, 225, 436, 666, 816, 1149, 1207, 1296
FauI CCCGC 2 cut(s) 1163, 1282
FauNDI CATATG 1 cut(s) 586
FbaI TGATCA 1 cut(s) 118
FblI GTMKAC 1 cut(s) 261
Fnu4HI GCNGC 8 cut(s) 169, 220, 223, 252, 409, 813, 1054, 1292
FokI GGATG 5 cut(s) 627, 683, 1148, 1322, 1394
FriOI GRGCYC 3 cut(s) 141, 1262, 1442
Fsp4HI GCNGC 8 cut(s) 169, 220, 223, 252, 409, 813, 1054, 1292
FspBI CTAG 1 cut(s) 861
FspI TGCGCA 1 cut(s) 441
GlaI GCGC 1 cut(s) 441
GluI GCNGC 8 cut(s) 169, 220, 223, 252, 409, 813, 1054, 1292
GsuI CTGGAG 2 cut(s) 640, 656
HaeIII GGCC 6 cut(s) 161, 168, 530, 752, 1020, 1187
HapII CCGG 7 cut(s) 268, 301, 644, 786, 964, 1017, 1109
HgaI GACGC 2 cut(s) 440, 1109
HhaI GCGC 1 cut(s) 442
Hin1II CATG 8 cut(s) 43, 229, 440, 670, 820, 1153, 1211, 1300
Hin6I GCGC 1 cut(s) 440
HinP1I GCGC 1 cut(s) 440
HincII GTYRAC 1 cut(s) 1008
HindII GTYRAC 1 cut(s) 1008
HinfI GANTC 6 cut(s) 22, 486, 500, 605, 1068, 1466
HpaI GTTAAC 1 cut(s) 1008
HpaII CCGG 7 cut(s) 268, 301, 644, 786, 964, 1017, 1109
HphI GGTGA 6 cut(s) 76, 169, 316, 365, 1072, 1261
Hpy166II GTNNAC 5 cut(s) 262, 782, 1008, 1080, 1141
Hpy188I TCNGA 5 cut(s) 193, 616, 880, 1402, 1480
Hpy188III TCNNGA 9 cut(s) 268, 340, 635, 704, 1177, 1297, 1325, 1345, 1409
Hpy8I GTNNAC 5 cut(s) 262, 782, 1008, 1080, 1141
Hpy99I CGWCG 2 cut(s) 212, 380
HpyAV CCTTC 6 cut(s) 172, 733, 742, 1322, 1351, 1379
HpyCH4III ACNGT 5 cut(s) 296, 337, 371, 469, 914
HpyCH4V TGCA 6 cut(s) 408, 945, 958, 1141, 1161, 1207
HpyF10VI GCNNNNNNNGC 8 cut(s) 448, 517, 671, 801, 818, 1167, 1283, 1319
HpyF3I CTNAG 3 cut(s) 67, 1287, 1441
Hsp92II CATG 8 cut(s) 43, 229, 440, 670, 820, 1153, 1211, 1300
HspAI GCGC 1 cut(s) 440
Kpn2I TCCGGA 1 cut(s) 267
Ksp22I TGATCA 1 cut(s) 118
KspAI GTTAAC 1 cut(s) 1008
KspI CCGCGG 1 cut(s) 1278
Kzo9I GATC 9 cut(s) 118, 126, 276, 589, 690, 700, 982, 1179, 1417
LmnI GCTCC 4 cut(s) 144, 637, 853, 1437
Lsp1109I GCAGC 6 cut(s) 206, 263, 420, 799, 1040, 1303
LweI GCATC 5 cut(s) 62, 649, 661, 1300, 1471
MaeI CTAG 1 cut(s) 861
MaeIII GTNAC 2 cut(s) 337, 1267
MalI GATC 9 cut(s) 120, 128, 278, 591, 692, 702, 984, 1181, 1419
MboI GATC 9 cut(s) 118, 126, 276, 589, 690, 700, 982, 1179, 1417
MboII GAAGA 8 cut(s) 166, 337, 368, 881, 992, 1312, 1373, 1461
MflI RGATCY 2 cut(s) 700, 1179
MhlI GDGCHC 4 cut(s) 141, 1143, 1262, 1442
MluCI AATT 6 cut(s) 13, 495, 610, 823, 1374, 1404
MlyI GAGTC 3 cut(s) 31, 495, 509
MmeI TCCRAC 1 cut(s) 216
Mph1103I ATGCAT 1 cut(s) 1209
MroI TCCGGA 1 cut(s) 267
MseI TTAA 5 cut(s) 540, 681, 716, 1007, 1250
MslI CAYNNNNRTG 2 cut(s) 38, 1148
MspA1I CMGCKG 3 cut(s) 254, 411, 1277
MspI CCGG 7 cut(s) 268, 301, 644, 786, 964, 1017, 1109
MspR9I CCNGG 7 cut(s) 302, 754, 786, 787, 965, 1109, 1125
Mva1269I GAATGC 1 cut(s) 89
MvaI CCWGG 2 cut(s) 754, 1125
MvnI CGCG 1 cut(s) 1277
MwoI GCNNNNNNNGC 8 cut(s) 448, 517, 671, 801, 818, 1167, 1283, 1319
NciI CCSGG 5 cut(s) 302, 786, 787, 965, 1109
NcoI CCATGG 2 cut(s) 666, 816
NdeI CATATG 1 cut(s) 586
NdeII GATC 9 cut(s) 118, 126, 276, 589, 690, 700, 982, 1179, 1417
NlaIII CATG 8 cut(s) 43, 229, 440, 670, 820, 1153, 1211, 1300
NlaIV GGNNCC 1 cut(s) 315
NmeAIII GCCGAG 4 cut(s) 137, 298, 556, 700
NmuCI GTSAC 2 cut(s) 337, 1267
NsbI TGCGCA 1 cut(s) 441
NsiI ATGCAT 1 cut(s) 1209
NspI RCATGY 2 cut(s) 43, 1153
PagI TCATGA 1 cut(s) 1296
PceI AGGCCT 1 cut(s) 752
PciI ACATGT 1 cut(s) 1149
PctI GAATGC 1 cut(s) 89
PfeI GAWTC 3 cut(s) 605, 1068, 1466
PkrI GCNGC 8 cut(s) 170, 221, 224, 253, 410, 814, 1055, 1293
PleI GAGTC 3 cut(s) 30, 494, 508
PpsI GAGTC 3 cut(s) 30, 494, 508
PscI ACATGT 1 cut(s) 1149
PsiI TTATAA 1 cut(s) 735
Psp124BI GAGCTC 3 cut(s) 141, 1262, 1442
Psp6I CCWGG 2 cut(s) 752, 1123
PspEI GGTNACC 1 cut(s) 1267
PspGI CCWGG 2 cut(s) 752, 1123
PspN4I GGNNCC 1 cut(s) 315
PspPI GGNCC 4 cut(s) 103, 270, 528, 779
PsuI RGATCY 2 cut(s) 700, 1179
RseI CAYNNNNRTG 2 cut(s) 38, 1148
SacI GAGCTC 3 cut(s) 141, 1262, 1442
SacII CCGCGG 1 cut(s) 1278
SaqAI TTAA 5 cut(s) 540, 681, 716, 1007, 1250
SatI GCNGC 8 cut(s) 169, 220, 223, 252, 409, 813, 1054, 1292
Sau3AI GATC 9 cut(s) 118, 126, 276, 589, 690, 700, 982, 1179, 1417
Sau96I GGNCC 4 cut(s) 103, 270, 528, 779
SchI GAGTC 3 cut(s) 31, 495, 509
ScrFI CCNGG 7 cut(s) 302, 754, 786, 787, 965, 1109, 1125
SduI GDGCHC 4 cut(s) 141, 1143, 1262, 1442
SfaNI GCATC 5 cut(s) 62, 649, 661, 1300, 1471
SfcI CTRYAG 1 cut(s) 568
Sfr303I CCGCGG 1 cut(s) 1278
SgrBI CCGCGG 1 cut(s) 1278
SinI GGWCC 3 cut(s) 103, 270, 779
SmaI CCCGGG 1 cut(s) 787
SmiMI CAYNNNNRTG 2 cut(s) 38, 1148
SmlI CTYRAG 2 cut(s) 830, 1485
SmoI CTYRAG 2 cut(s) 830, 1485
SpeI ACTAGT 1 cut(s) 860
Sse9I AATT 6 cut(s) 13, 495, 610, 823, 1374, 1404
SseBI AGGCCT 1 cut(s) 752
SspMI CTAG 1 cut(s) 861
SstI GAGCTC 3 cut(s) 141, 1262, 1442
StuI AGGCCT 1 cut(s) 752
StyD4I CCNGG 7 cut(s) 300, 752, 784, 785, 963, 1107, 1123
StyI CCWWGG 3 cut(s) 666, 816, 1198
TaaI ACNGT 5 cut(s) 296, 337, 371, 469, 914
TaqI TCGA 5 cut(s) 210, 375, 484, 626, 1129
TasI AATT 6 cut(s) 13, 495, 610, 823, 1374, 1404
TauI GCSGC 2 cut(s) 171, 225
TfiI GAWTC 3 cut(s) 605, 1068, 1466
Tru1I TTAA 5 cut(s) 540, 681, 716, 1007, 1250
Tru9I TTAA 5 cut(s) 540, 681, 716, 1007, 1250
TscAI CASTG 2 cut(s) 474, 952
TseFI GTSAC 2 cut(s) 337, 1267
TseI GCWGC 6 cut(s) 219, 251, 408, 812, 1053, 1291
Tsp45I GTSAC 2 cut(s) 337, 1267
TspDTI ATGAA 6 cut(s) 82, 618, 1224, 1313, 1398, 1473
TspGWI ACGGA 2 cut(s) 95, 582
TspMI CCCGGG 1 cut(s) 785
TspRI CASTG 2 cut(s) 474, 952
VneI GTGCAC 1 cut(s) 1139
VpaK11BI GGWCC 3 cut(s) 103, 270, 779
XapI RAATTY 1 cut(s) 1404
XceI RCATGY 2 cut(s) 43, 1153
XmaI CCCGGG 1 cut(s) 785
XmiI GTMKAC 1 cut(s) 261
XspI CTAG 1 cut(s) 861
Zsp2I ATGCAT 1 cut(s) 1209
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.