Rorug02G0284100

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0021621
Physical Location & Seq
Reverse (-)
30893887 .. 30898546
4660 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0284100.1

Sequence Viewer

Length: 1458 bp
ATGATGGCTCTCTGGGTCAGGAAATCACGAGACTTTATGATCAACCGTTTCAAGTCCAAGATGGGTTCTTCGGGTTTGATCCATTTCAGCTCTTGCAGTTCATCTTTCACTCGCAAATCTCCGGTCCTGGTTCCTTTTCCGGCTTCATTTGCTCAAAACCACAGAATCCCGGAGGGAGAGTTTGGAAACTCGCCGGCCCATTTCCGTTTCTGCTCTCGTCGTTTTGAATCCGGTAAAGCAGAAACGCTCTACGACGAGGGTGATGCCAATCAGGTTTTGGATTTACCAGATGGGAAGGTCAAATTTACTCCTGAAATGAGGTTCATATCTGAGTCGACTAAGGAGCGCGAGTCCTGTTATCGGGTCCTTGACGACAATGGGAAGAAAATTATGTCCAGCAACTATGTTGAGGTCAGCCGGGAAGTTGCTGTAAAAATGTACACAGACATGGTGATGCTTCAGACTATGGACACCATTTTCTACGAAGCACAGAGGCAAGGAAGGATTTCCTTTTATCTGACTACAGTTGGTGAAGAAGCCATCAATATTGCATCTGCTGCAGCACTCACCTTTGATGACATTGTCCTTCCTCAGTACAGGGAGCCAGGAGTTTTAATATGGCGTGGTTTCACTGCCCAAGAATTTGCAAACCAGTGTTTCAGTAACAAAGCTGATTATGGGAAAGGCAGGCAGATGCCAATCCATTATGGGTCTAACAAGCACAATTACATCACTGTTTCATCACCCATAGCTACACAACTTCCACAGGCTGTTGGTGTTGCTTATTCTCTAAAAATGGACAGAAAAGATGCATGTGTGGTCACATATATTGGTGAAGGTGGCACAAGTGAGGGTGATTTCCATGCTGCTCTAAATTTTGCGGCTGTTACGGAGGCTCCAGTCATATTCATTTGTCGGAACAACGGATGGGCTATCAGTACACCTACTTCAGACCAGTTTCGGAGTGATGGTGTTGTTGTCAAAGGTCGGGCTTATGGAATCCGAAGTATCCGAGTAGATGGGAATGATGCACTTGCCATGTATAGTGCAGTTTATGCTGCACGCGAAATGGCAATCAGTGAACAGAGACCAATCTTAATAGAGGCATTAACATATCGAGTTGGACACCATTCCACATCAGATGATTCAACCAAGTATCGGCCAGTTGATGAGATTGAACGGTGGAAAGTGGAACGAGACCCAGTAACTAGATTTAGAAAATGGATTGAAAGAAATGGTTGGTGGACTGGTGAGGCAGAGAAAGAGGCTAGAAACAGTGCAAGAAAACAGATGTTACATGCAATCCAAGAAGCTGAGAAAGTTGAAAAGCCACCAGTCGCTGACATGTTCACAGATGTATATGACTGTCTTCCTTCCAATCTTGTTGAGCAGGAGAAACGGCTAAGAGAGACAATTAAGAGACACCCCCAGGATTACCCATCTGATGTTCCTGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

485

Amino Acids

55.03

Weight (kDa)

7.14

Isoelectric Point (pI)

48.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
E1_dh PF00676 149 - 445 5.3e-95 Dehydrogenase E1 component
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0021621)

Species Orthologous Gene IDs
rosa_multiflora Rmu_ssc0000449.1_g000010
rosa_rugosa Rorug02G0284100
rosa_samantha Rh2AG336600 Rh2CG323900 Rh2DG361500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 335
AccII CGCG 2 cut(s) 348, 1065
AciI CCGC 1 cut(s) 881
AclWI GGATC 1 cut(s) 73
AcoI YGGCCR 1 cut(s) 1160
AcsI RAATTY 3 cut(s) 302, 641, 874
AcuI CTGAAG 2 cut(s) 443, 933
AfaI GTAC 3 cut(s) 440, 596, 940
AfiI CCNNNNNNNGG 2 cut(s) 360, 1158
AflIII ACRYGT 1 cut(s) 1344
AgsI TTSAA 6 cut(s) 52, 227, 1149, 1178, 1229, 1325
AjnI CCWGG 3 cut(s) 126, 604, 1428
AluBI AGCT 4 cut(s) 90, 671, 752, 1313
AluI AGCT 4 cut(s) 90, 671, 752, 1313
Alw26I GTCTC 5 cut(s) 24, 1081, 1191, 1403, 1414
AlwI GGATC 1 cut(s) 73
AlwNI CAGNNNCTG 1 cut(s) 1340
AoxI GGCC 2 cut(s) 195, 1160
ApeKI GCWGC 4 cut(s) 557, 560, 866, 1058
ApoI RAATTY 3 cut(s) 302, 641, 874
ArsI GACNNNNNNTTYG 2 cut(s) 108, 140
Asp700I GAANNNNTTC 1 cut(s) 505
AspLEI GCGC 1 cut(s) 348
AspS9I GGNCC 3 cut(s) 124, 196, 364
AsuC2I CCSGG 2 cut(s) 170, 419
AsuHPI GGTGA 8 cut(s) 272, 463, 542, 559, 735, 845, 866, 1262
AvaII GGWCC 2 cut(s) 124, 364
BarI GAAGNNNNNNTAC 2 cut(s) 931, 963
BauI CACGAG 1 cut(s) 27
BbsI GAAGAC 1 cut(s) 1361
BbvI GCAGC 4 cut(s) 544, 572, 853, 1045
BccI CCATC 7 cut(s) 55, 284, 548, 921, 962, 1013, 1447
BceAI ACGGC 1 cut(s) 1415
BcgI CGANNNNNNTGC 2 cut(s) 245, 279
BciT130I CCWGG 3 cut(s) 128, 606, 1430
BciVI GTATCC 1 cut(s) 1019
BclI TGATCA 1 cut(s) 39
BcnI CCSGG 2 cut(s) 170, 419
BcoDI GTCTC 5 cut(s) 24, 1081, 1191, 1403, 1414
BfaI CTAG 2 cut(s) 1209, 1269
BfmI CTRYAG 2 cut(s) 522, 558
BfuI GTATCC 1 cut(s) 1019
BisI GCNGC 5 cut(s) 558, 561, 867, 882, 1059
BlsI GCNGC 5 cut(s) 559, 562, 868, 883, 1060
Bme1390I CCNGG 5 cut(s) 128, 170, 419, 606, 1430
Bme18I GGWCC 2 cut(s) 124, 364
BmgT120I GGNCC 3 cut(s) 124, 196, 364
BmiI GGNNCC 4 cut(s) 132, 365, 603, 897
BmrFI CCNGG 5 cut(s) 128, 170, 419, 606, 1430
BmrI ACTGGG 1 cut(s) 1196
BmsI GCATC 6 cut(s) 253, 444, 560, 684, 799, 1018
BmuI ACTGGG 1 cut(s) 1196
BpiI GAAGAC 1 cut(s) 1361
BpmI CTGGAG 1 cut(s) 882
BpuMI CCSGG 2 cut(s) 170, 419
BsaBI GATNNNNATC 2 cut(s) 267, 698
BsaI GGTCTC 2 cut(s) 1081, 1191
BsaJI CCNNGG 1 cut(s) 1428
BsaWI WCCGGW 2 cut(s) 121, 230
BsaXI ACNNNNNCTCC 8 cut(s) 164, 194, 335, 365, 880, 910, 955, 985
Bsc4I CCNNNNNNNGG 2 cut(s) 360, 1158
Bse118I RCCGGY 1 cut(s) 193
Bse1I ACTGG 7 cut(s) 652, 899, 955, 1163, 1202, 1252, 1334
Bse8I GATNNNNATC 2 cut(s) 267, 698
BseBI CCWGG 3 cut(s) 128, 606, 1430
BseDI CCNNGG 1 cut(s) 1428
BseGI GGATG 1 cut(s) 932
BseJI GATNNNNATC 2 cut(s) 267, 698
BseLI CCNNNNNNNGG 2 cut(s) 360, 1158
BseMII CTCAG 3 cut(s) 321, 605, 1305
BseNI ACTGG 7 cut(s) 652, 899, 955, 1163, 1202, 1252, 1334
BseXI GCAGC 4 cut(s) 544, 572, 853, 1045
BsgI GTGCAG 2 cut(s) 1044, 1068
Bsh1236I CGCG 2 cut(s) 348, 1065
BshFI GGCC 2 cut(s) 197, 1162
BsiSI CCGG 6 cut(s) 122, 140, 170, 194, 231, 418
BslI CCNNNNNNNGG 2 cut(s) 360, 1158
BsmAI GTCTC 5 cut(s) 24, 1081, 1191, 1403, 1414
BsnI GGCC 2 cut(s) 197, 1162
Bso31I GGTCTC 2 cut(s) 1081, 1191
Bsp1407I TGTACA 1 cut(s) 438
Bsp143I GATC 2 cut(s) 39, 78
BspACI CCGC 1 cut(s) 881
BspANI GGCC 2 cut(s) 197, 1162
BspCNI CTCAG 3 cut(s) 322, 604, 1306
BspFNI CGCG 2 cut(s) 348, 1065
BspLI GGNNCC 4 cut(s) 132, 365, 603, 897
BspMAI CTGCAG 1 cut(s) 562
BspPI GGATC 1 cut(s) 73
BspTNI GGTCTC 2 cut(s) 1081, 1191
BsrFI RCCGGY 1 cut(s) 193
BsrGI TGTACA 1 cut(s) 438
BsrI ACTGG 7 cut(s) 652, 899, 955, 1163, 1202, 1252, 1334
BssAI RCCGGY 1 cut(s) 193
BssECI CCNNGG 1 cut(s) 1428
BssMI GATC 2 cut(s) 39, 78
BssSI CACGAG 1 cut(s) 27
Bst2BI CACGAG 1 cut(s) 27
Bst2UI CCWGG 3 cut(s) 128, 606, 1430
Bst4CI ACNGT 6 cut(s) 47, 526, 736, 1182, 1277, 1367
BstAPI GCANNNNNTGC 2 cut(s) 557, 1055
BstAUI TGTACA 1 cut(s) 438
BstC8I GCNNGC 3 cut(s) 195, 689, 1063
BstDEI CTNAG 5 cut(s) 330, 339, 591, 1314, 1403
BstF5I GGATG 1 cut(s) 932
BstFNI CGCG 2 cut(s) 348, 1065
BstHHI GCGC 1 cut(s) 348
BstKTI GATC 2 cut(s) 42, 81
BstMAI GTCTC 5 cut(s) 24, 1081, 1191, 1403, 1414
BstMBI GATC 2 cut(s) 39, 78
BstMWI GCNNNNNNNGC 3 cut(s) 149, 557, 1055
BstNI CCWGG 3 cut(s) 128, 606, 1430
BstNSI RCATGY 3 cut(s) 816, 1301, 1348
BstSCI CCNGG 5 cut(s) 126, 168, 417, 604, 1428
BstSFI CTRYAG 2 cut(s) 522, 558
BstUI CGCG 2 cut(s) 348, 1065
BstV1I GCAGC 4 cut(s) 544, 572, 853, 1045
BstV2I GAAGAC 1 cut(s) 1361
BsuI GTATCC 1 cut(s) 1019
BsuRI GGCC 2 cut(s) 197, 1162
BtsCI GGATG 1 cut(s) 932
BtsI GCAGTG 1 cut(s) 630
BtsIMutI CAGTG 5 cut(s) 630, 659, 732, 1084, 1282
Cac8I GCNNGC 3 cut(s) 195, 689, 1063
CaiI CAGNNNCTG 1 cut(s) 1340
CfoI GCGC 1 cut(s) 348
Cfr10I RCCGGY 1 cut(s) 193
Cfr13I GGNCC 3 cut(s) 124, 196, 364
Csp6I GTAC 3 cut(s) 439, 595, 939
CviAII CATG 6 cut(s) 448, 813, 863, 1039, 1298, 1345
CviQI GTAC 3 cut(s) 439, 595, 939
DdeI CTNAG 5 cut(s) 330, 339, 591, 1314, 1403
DpnI GATC 2 cut(s) 41, 80
DpnII GATC 2 cut(s) 39, 78
EaeI YGGCCR 1 cut(s) 1160
Eco31I GGTCTC 2 cut(s) 1081, 1191
Eco47I GGWCC 2 cut(s) 124, 364
Eco57I CTGAAG 2 cut(s) 443, 933
EcoO109I RGGNCCY 1 cut(s) 364
EcoRII CCWGG 3 cut(s) 126, 604, 1428
EcoT22I ATGCAT 1 cut(s) 814
FaeI CATG 6 cut(s) 451, 816, 866, 1042, 1301, 1348
FatI CATG 6 cut(s) 447, 812, 862, 1038, 1297, 1344
FbaI TGATCA 1 cut(s) 39
FblI GTMKAC 1 cut(s) 335
Fnu4HI GCNGC 5 cut(s) 558, 561, 867, 882, 1059
FokI GGATG 1 cut(s) 939
Fsp4HI GCNGC 5 cut(s) 558, 561, 867, 882, 1059
FspBI CTAG 2 cut(s) 1209, 1269
GlaI GCGC 1 cut(s) 347
GluI GCNGC 5 cut(s) 558, 561, 867, 882, 1059
GsuI CTGGAG 1 cut(s) 882
HaeIII GGCC 2 cut(s) 197, 1162
HapII CCGG 6 cut(s) 122, 140, 170, 194, 231, 418
HhaI GCGC 1 cut(s) 348
Hin1II CATG 6 cut(s) 451, 816, 866, 1042, 1301, 1348
Hin6I GCGC 1 cut(s) 346
HinP1I GCGC 1 cut(s) 346
HincII GTYRAC 1 cut(s) 336
HindII GTYRAC 1 cut(s) 336
HinfI GANTC 6 cut(s) 165, 227, 332, 350, 999, 1145
HpaII CCGG 6 cut(s) 122, 140, 170, 194, 231, 418
HphI GGTGA 8 cut(s) 272, 463, 542, 559, 735, 845, 866, 1262
Hpy166II GTNNAC 6 cut(s) 336, 441, 941, 1082, 1245, 1350
Hpy188III TCNNGA 3 cut(s) 19, 27, 311
Hpy8I GTNNAC 6 cut(s) 336, 441, 941, 1082, 1245, 1350
Hpy99I CGWCG 2 cut(s) 222, 257
HpyAV CCTTC 5 cut(s) 289, 495, 596, 830, 1383
HpyCH4III ACNGT 6 cut(s) 47, 526, 736, 1182, 1277, 1367
HpyF10VI GCNNNNNNNGC 3 cut(s) 149, 557, 1055
HpyF3I CTNAG 5 cut(s) 330, 339, 591, 1314, 1403
Hsp92II CATG 6 cut(s) 451, 816, 866, 1042, 1301, 1348
HspAI GCGC 1 cut(s) 346
KroI GCCGGC 1 cut(s) 193
KroNI GCCGGC 1 cut(s) 195
Ksp22I TGATCA 1 cut(s) 39
Kzo9I GATC 2 cut(s) 39, 78
LmnI GCTCC 3 cut(s) 343, 601, 901
Lsp1109I GCAGC 4 cut(s) 544, 572, 853, 1045
LweI GCATC 6 cut(s) 253, 444, 560, 684, 799, 1018
MaeI CTAG 2 cut(s) 1209, 1269
MaeIII GTNAC 5 cut(s) 662, 820, 886, 1204, 1293
MalI GATC 2 cut(s) 41, 80
MboI GATC 2 cut(s) 39, 78
MboII GAAGA 4 cut(s) 60, 394, 545, 1361
MluCI AATT 6 cut(s) 302, 387, 641, 724, 874, 1413
MlyI GAGTC 2 cut(s) 341, 359
MmeI TCCRAC 2 cut(s) 896, 1102
Mph1103I ATGCAT 1 cut(s) 814
MroNI GCCGGC 1 cut(s) 193
MroXI GAANNNNTTC 1 cut(s) 505
MseI TTAA 4 cut(s) 614, 1097, 1109, 1416
MslI CAYNNNNRTG 2 cut(s) 446, 452
MspI CCGG 6 cut(s) 122, 140, 170, 194, 231, 418
MspR9I CCNGG 5 cut(s) 128, 170, 419, 606, 1430
MvaI CCWGG 3 cut(s) 128, 606, 1430
MvnI CGCG 2 cut(s) 348, 1065
MwoI GCNNNNNNNGC 3 cut(s) 149, 557, 1055
NaeI GCCGGC 1 cut(s) 195
NciI CCSGG 2 cut(s) 170, 419
NdeII GATC 2 cut(s) 39, 78
NgoMIV GCCGGC 1 cut(s) 193
NlaIII CATG 6 cut(s) 451, 816, 866, 1042, 1301, 1348
NlaIV GGNNCC 4 cut(s) 132, 365, 603, 897
NmuCI GTSAC 1 cut(s) 820
NsiI ATGCAT 1 cut(s) 814
NspI RCATGY 3 cut(s) 816, 1301, 1348
PciI ACATGT 1 cut(s) 1344
PdiI GCCGGC 1 cut(s) 195
PdmI GAANNNNTTC 1 cut(s) 505
PfeI GAWTC 4 cut(s) 165, 227, 999, 1145
PflFI GACNNNGTC 1 cut(s) 581
PfoI TCCNGGA 1 cut(s) 168
PkrI GCNGC 5 cut(s) 559, 562, 868, 883, 1060
PleI GAGTC 2 cut(s) 340, 358
PpsI GAGTC 2 cut(s) 340, 358
PpuMI RGGWCCY 1 cut(s) 364
PscI ACATGT 1 cut(s) 1344
Psp5II RGGWCCY 1 cut(s) 364
Psp6I CCWGG 3 cut(s) 126, 604, 1428
PspGI CCWGG 3 cut(s) 126, 604, 1428
PspN4I GGNNCC 4 cut(s) 132, 365, 603, 897
PspPI GGNCC 3 cut(s) 124, 196, 364
PspPPI RGGWCCY 1 cut(s) 364
PstI CTGCAG 1 cut(s) 562
PstNI CAGNNNCTG 1 cut(s) 1340
PsyI GACNNNGTC 1 cut(s) 581
RsaI GTAC 3 cut(s) 440, 596, 940
RsaNI GTAC 3 cut(s) 439, 595, 939
RseI CAYNNNNRTG 2 cut(s) 446, 452
SalI GTCGAC 1 cut(s) 334
SaqAI TTAA 4 cut(s) 614, 1097, 1109, 1416
SatI GCNGC 5 cut(s) 558, 561, 867, 882, 1059
Sau3AI GATC 2 cut(s) 39, 78
Sau96I GGNCC 3 cut(s) 124, 196, 364
SchI GAGTC 2 cut(s) 341, 359
ScrFI CCNGG 5 cut(s) 128, 170, 419, 606, 1430
SfaNI GCATC 6 cut(s) 253, 444, 560, 684, 799, 1018
SfcI CTRYAG 2 cut(s) 522, 558
SinI GGWCC 2 cut(s) 124, 364
SmiMI CAYNNNNRTG 2 cut(s) 446, 452
Sse9I AATT 6 cut(s) 302, 387, 641, 724, 874, 1413
SsiI CCGC 1 cut(s) 881
SspI AATATT 1 cut(s) 547
SspMI CTAG 2 cut(s) 1209, 1269
StyD4I CCNGG 5 cut(s) 126, 168, 417, 604, 1428
TaaI ACNGT 6 cut(s) 47, 526, 736, 1182, 1277, 1367
TaqI TCGA 2 cut(s) 335, 1117
TasI AATT 6 cut(s) 302, 387, 641, 724, 874, 1413
TatI WGTACW 3 cut(s) 438, 594, 938
TauI GCSGC 1 cut(s) 884
TfiI GAWTC 4 cut(s) 165, 227, 999, 1145
Tru1I TTAA 4 cut(s) 614, 1097, 1109, 1416
Tru9I TTAA 4 cut(s) 614, 1097, 1109, 1416
TscAI CASTG 5 cut(s) 637, 659, 739, 1084, 1282
TseFI GTSAC 1 cut(s) 820
TseI GCWGC 4 cut(s) 557, 560, 866, 1058
Tsp45I GTSAC 1 cut(s) 820
TspDTI ATGAA 5 cut(s) 90, 135, 313, 729, 898
TspGWI ACGGA 3 cut(s) 194, 905, 939
TspRI CASTG 5 cut(s) 637, 659, 739, 1084, 1282
Tth111I GACNNNGTC 1 cut(s) 581
VpaK11BI GGWCC 2 cut(s) 124, 364
XapI RAATTY 3 cut(s) 302, 641, 874
XceI RCATGY 3 cut(s) 816, 1301, 1348
XcmI CCANNNNNNNNNTGG 1 cut(s) 274
XmiI GTMKAC 1 cut(s) 335
XmnI GAANNNNTTC 1 cut(s) 505
XspI CTAG 2 cut(s) 1209, 1269
Zsp2I ATGCAT 1 cut(s) 814
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.