Rorug02G0588200

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0016614
Physical Location & Seq
Reverse (-)
71050643 .. 71051056
414 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0588200.1

Sequence Viewer

Length: 414 bp
ATGTCCCACCAAAATCTTGTTCCTTCTGGCCCGCCCACCACAGTCTTCCCTGCAGTACGCATCAATGTTCAATTTGAACTCGAACGTGCTAACATAGGCCGGAACAACAAACGATGGCCGCTAGTGCTGCCGGCATCCAAGGCCTCCATAGACGCCATGGCGAAAGTGGAAGTGACGTCAGAGGCAGGGCCATCGGAGTGCAGTATCTGCTTGGAAGTCTTGAAGGTGGGCTGTGAAGCCAATGAGATGCCTAACTGTAAGCACAGGTTTCACTCGGCTTGTATCGAAACCTGGCTTCTCCGGCATGATTCATGCCCGCTTTGCAGGTCCACTATGCCAATACTAGAGGAAGATATCAGGCCAACGGCTGGAATAGCGATTACCAAGCTGATGATCGTAATAGCATGCAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

137

Amino Acids

15.08

Weight (kDa)

6.88

Isoelectric Point (pI)

73.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 66 - 109 8.3e-12 Ring finger domain
zf-C3HC4_2 PF13923 66 - 108 6.3e-09 Zinc finger, C3HC4 type (RING finger)
zf-RING_11 PF17123 66 - 95 3.3e-08 RING-like zinc finger
zf-C3HC4 PF00097 67 - 108 1.3e-07 Zinc finger, C3HC4 type (RING finger)
zf-rbx1 PF12678 67 - 109 5.4e-07 RING-H2 zinc finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016614)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G01960
fragaria_vesca FvH4_6g53750
prunus_persica Prupe.3G308000_v2.0.a1
pyrus_communis pycom17g00770
rosa_chinensis RchiOBHm_Chr2g0175871
rosa_laevigata RLG00000022361
rosa_multiflora Rmu_sc0003282.1_g000001
rosa_roxburghii Rroxscaffold_2G00076980
rosa_rugosa Rorug02G0588200
rosa_samantha Rh2AG667500 Rh2BG679000 Rh2CG641800 Rh2DG692500
rosa_wichuraiana Rw2G054710

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 179
Acc36I ACCTGC 1 cut(s) 315
AccB7I CCANNNNNTGG 1 cut(s) 368
AciI CCGC 3 cut(s) 32, 119, 317
AcoI YGGCCR 1 cut(s) 116
AcyI GRCGYC 2 cut(s) 153, 176
AfaI GTAC 1 cut(s) 57
AfiI CCNNNNNNNGG 1 cut(s) 368
AgsI TTSAA 3 cut(s) 71, 77, 223
AjnI CCWGG 1 cut(s) 290
AjuI GAANNNNNNNTTGG 1 cut(s) 35
AluBI AGCT 1 cut(s) 388
AluI AGCT 1 cut(s) 388
AlwNI CAGNNNCTG 1 cut(s) 207
AoxI GGCC 6 cut(s) 28, 97, 116, 141, 188, 359
ApeKI GCWGC 1 cut(s) 127
AspS9I GGNCC 3 cut(s) 29, 188, 327
AvaII GGWCC 1 cut(s) 327
BbsI GAAGAC 1 cut(s) 37
BbvI GCAGC 1 cut(s) 114
BccI CCATC 2 cut(s) 108, 199
BceAI ACGGC 1 cut(s) 381
BcgI CGANNNNNNTGC 2 cut(s) 174, 208
BciT130I CCWGG 1 cut(s) 292
BfaI CTAG 2 cut(s) 122, 344
BfmI CTRYAG 1 cut(s) 51
BfuAI ACCTGC 1 cut(s) 315
BisI GCNGC 2 cut(s) 119, 128
BlsI GCNGC 2 cut(s) 120, 129
Bme1390I CCNGG 1 cut(s) 292
Bme18I GGWCC 1 cut(s) 327
BmgT120I GGNCC 3 cut(s) 29, 188, 327
BmrFI CCNGG 1 cut(s) 292
BmsI GCATC 3 cut(s) 69, 143, 237
BpiI GAAGAC 1 cut(s) 37
BsaHI GRCGYC 2 cut(s) 153, 176
BsaJI CCNNGG 2 cut(s) 138, 156
Bsc4I CCNNNNNNNGG 1 cut(s) 368
Bse118I RCCGGY 1 cut(s) 130
BseBI CCWGG 1 cut(s) 292
BseDI CCNNGG 2 cut(s) 138, 156
BseGI GGATG 1 cut(s) 134
BseLI CCNNNNNNNGG 1 cut(s) 368
BseXI GCAGC 1 cut(s) 114
BsgI GTGCAG 1 cut(s) 220
BshFI GGCC 6 cut(s) 30, 99, 118, 143, 190, 361
BsiSI CCGG 3 cut(s) 100, 131, 301
BslI CCNNNNNNNGG 1 cut(s) 368
BsnI GGCC 6 cut(s) 30, 99, 118, 143, 190, 361
Bsp143I GATC 1 cut(s) 393
Bsp19I CCATGG 1 cut(s) 156
BspACI CCGC 3 cut(s) 32, 119, 317
BspANI GGCC 6 cut(s) 30, 99, 118, 143, 190, 361
BspMAI CTGCAG 1 cut(s) 55
BspMI ACCTGC 1 cut(s) 315
BsrFI RCCGGY 1 cut(s) 130
BssAI RCCGGY 1 cut(s) 130
BssECI CCNNGG 2 cut(s) 138, 156
BssMI GATC 1 cut(s) 393
BssNI GRCGYC 2 cut(s) 153, 176
BssT1I CCWWGG 2 cut(s) 138, 156
Bst2UI CCWGG 1 cut(s) 292
Bst4CI ACNGT 2 cut(s) 43, 257
BstACI GRCGYC 2 cut(s) 153, 176
BstAPI GCANNNNNTGC 1 cut(s) 207
BstC8I GCNNGC 4 cut(s) 32, 132, 317, 406
BstDSI CCRYGG 1 cut(s) 156
BstF5I GGATG 1 cut(s) 134
BstKTI GATC 1 cut(s) 396
BstMBI GATC 1 cut(s) 393
BstMWI GCNNNNNNNGC 7 cut(s) 124, 127, 140, 207, 301, 321, 374
BstNI CCWGG 1 cut(s) 292
BstNSI RCATGY 1 cut(s) 408
BstSCI CCNGG 1 cut(s) 290
BstSFI CTRYAG 1 cut(s) 51
BstV1I GCAGC 1 cut(s) 114
BstV2I GAAGAC 1 cut(s) 37
BsuRI GGCC 6 cut(s) 30, 99, 118, 143, 190, 361
BtgI CCRYGG 1 cut(s) 156
BtsCI GGATG 1 cut(s) 134
BveI ACCTGC 1 cut(s) 315
Cac8I GCNNGC 4 cut(s) 32, 132, 317, 406
CaiI CAGNNNCTG 1 cut(s) 207
Cfr10I RCCGGY 1 cut(s) 130
Cfr13I GGNCC 3 cut(s) 29, 188, 327
CseI GACGC 1 cut(s) 161
Csp6I GTAC 1 cut(s) 56
CviAII CATG 4 cut(s) 157, 305, 312, 405
CviQI GTAC 1 cut(s) 56
DpnI GATC 1 cut(s) 395
DpnII GATC 1 cut(s) 393
EaeI YGGCCR 1 cut(s) 116
Eco130I CCWWGG 2 cut(s) 138, 156
Eco147I AGGCCT 1 cut(s) 143
Eco32I GATATC 1 cut(s) 355
Eco47I GGWCC 1 cut(s) 327
EcoRII CCWGG 1 cut(s) 290
EcoRV GATATC 1 cut(s) 355
EcoT14I CCWWGG 2 cut(s) 138, 156
ErhI CCWWGG 2 cut(s) 138, 156
FaeI CATG 4 cut(s) 160, 308, 315, 408
FaiI YATR 7 cut(s) 95, 149, 158, 306, 313, 335, 406
FatI CATG 4 cut(s) 156, 304, 311, 404
FauI CCCGC 2 cut(s) 39, 324
Fnu4HI GCNGC 2 cut(s) 119, 128
FokI GGATG 1 cut(s) 121
Fsp4HI GCNGC 2 cut(s) 119, 128
FspBI CTAG 2 cut(s) 122, 344
GluI GCNGC 2 cut(s) 119, 128
HaeIII GGCC 6 cut(s) 30, 99, 118, 143, 190, 361
HapII CCGG 3 cut(s) 100, 131, 301
HgaI GACGC 1 cut(s) 161
Hin1I GRCGYC 2 cut(s) 153, 176
Hin1II CATG 4 cut(s) 160, 308, 315, 408
HinfI GANTC 1 cut(s) 308
HpaII CCGG 3 cut(s) 100, 131, 301
Hpy166II GTNNAC 1 cut(s) 330
Hpy188I TCNGA 2 cut(s) 181, 196
Hpy188III TCNNGA 1 cut(s) 220
Hpy8I GTNNAC 1 cut(s) 330
HpyAV CCTTC 2 cut(s) 33, 217
HpyCH4III ACNGT 2 cut(s) 43, 257
HpyCH4IV ACGT 2 cut(s) 85, 176
HpyCH4V TGCA 4 cut(s) 53, 201, 324, 408
HpyF10VI GCNNNNNNNGC 7 cut(s) 124, 127, 140, 207, 301, 321, 374
HpySE526I ACGT 2 cut(s) 85, 176
Hsp92I GRCGYC 2 cut(s) 153, 176
Hsp92II CATG 4 cut(s) 160, 308, 315, 408
KroI GCCGGC 1 cut(s) 130
KroNI GCCGGC 1 cut(s) 132
Kzo9I GATC 1 cut(s) 393
Lsp1109I GCAGC 1 cut(s) 114
LweI GCATC 3 cut(s) 69, 143, 237
MaeI CTAG 2 cut(s) 122, 344
MaeII ACGT 2 cut(s) 85, 176
MaeIII GTNAC 1 cut(s) 172
MalI GATC 1 cut(s) 395
MboI GATC 1 cut(s) 393
MboII GAAGA 2 cut(s) 37, 362
MluCI AATT 1 cut(s) 71
MnlI CCTC 3 cut(s) 154, 175, 340
MroNI GCCGGC 1 cut(s) 130
MslI CAYNNNNRTG 1 cut(s) 196
MspI CCGG 3 cut(s) 100, 131, 301
MspR9I CCNGG 1 cut(s) 292
MvaI CCWGG 1 cut(s) 292
MwoI GCNNNNNNNGC 7 cut(s) 124, 127, 140, 207, 301, 321, 374
NaeI GCCGGC 1 cut(s) 132
NcoI CCATGG 1 cut(s) 156
NdeII GATC 1 cut(s) 393
NgoMIV GCCGGC 1 cut(s) 130
NlaIII CATG 4 cut(s) 160, 308, 315, 408
NmeAIII GCCGAG 1 cut(s) 254
NmuCI GTSAC 1 cut(s) 172
NspI RCATGY 1 cut(s) 408
PaeI GCATGC 1 cut(s) 408
PceI AGGCCT 1 cut(s) 143
PdiI GCCGGC 1 cut(s) 132
PfeI GAWTC 1 cut(s) 308
PflMI CCANNNNNTGG 1 cut(s) 368
PkrI GCNGC 2 cut(s) 120, 129
Psp6I CCWGG 1 cut(s) 290
PspGI CCWGG 1 cut(s) 290
PspPI GGNCC 3 cut(s) 29, 188, 327
PstI CTGCAG 1 cut(s) 55
PstNI CAGNNNCTG 1 cut(s) 207
RsaI GTAC 1 cut(s) 57
RsaNI GTAC 1 cut(s) 56
RseI CAYNNNNRTG 1 cut(s) 196
SatI GCNGC 2 cut(s) 119, 128
Sau3AI GATC 1 cut(s) 393
Sau96I GGNCC 3 cut(s) 29, 188, 327
ScrFI CCNGG 1 cut(s) 292
SetI ASST 7 cut(s) 88, 179, 228, 269, 293, 329, 390
SfaNI GCATC 3 cut(s) 69, 143, 237
SfcI CTRYAG 1 cut(s) 51
SinI GGWCC 1 cut(s) 327
SmiMI CAYNNNNRTG 1 cut(s) 196
SphI GCATGC 1 cut(s) 408
Sse9I AATT 1 cut(s) 71
SseBI AGGCCT 1 cut(s) 143
SsiI CCGC 3 cut(s) 32, 119, 317
SspMI CTAG 2 cut(s) 122, 344
StuI AGGCCT 1 cut(s) 143
StyD4I CCNGG 1 cut(s) 290
StyI CCWWGG 2 cut(s) 138, 156
TaaI ACNGT 2 cut(s) 43, 257
TaiI ACGT 2 cut(s) 88, 179
TaqI TCGA 2 cut(s) 81, 285
TasI AATT 1 cut(s) 71
TauI GCSGC 1 cut(s) 121
TfiI GAWTC 1 cut(s) 308
TseFI GTSAC 1 cut(s) 172
TseI GCWGC 1 cut(s) 127
Tsp45I GTSAC 1 cut(s) 172
TspDTI ATGAA 1 cut(s) 300
Van91I CCANNNNNTGG 1 cut(s) 368
VpaK11BI GGWCC 1 cut(s) 327
XceI RCATGY 1 cut(s) 408
XcmI CCANNNNNNNNNTGG 2 cut(s) 154, 163
XspI CTAG 2 cut(s) 122, 344
ZraI GACGTC 1 cut(s) 177
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.