Rorug03G0033000

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0018521
Physical Location & Seq
Reverse (-)
2634718 .. 2635233
516 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0033000.1

Sequence Viewer

Length: 516 bp
ATGAAGAGATTGAAGATAGACTGGGCTCCGTATGTTCCTTTGGACAAGAGAGTAGACAAAGAAGGTTCTGAAATCTTTGTCTTGAGCTGCAAGCAAAGAAGGGCTGCTCTCAAACACAAAAATGTCAAGGGAAACAATGATCTATTGAAGTTTGAATACGCCGTGCCTTATTTGGATCCAGCGGCAAAAGTAAAGTGTCATGAAGTTCCTGTTCAGGAGGAGGAGTACTTTAGTGCTAATGATGATGAGCTGGAGGAGTTGTACTTCAGTGGTGACGATGAGAACCAGGTGATAGAAGAAGATGATGAAAAGAATGTCATGGTGGAAGAAGAAGCAAAGGGAGAAAATATTGATCTTGGAGTTAGGGAAGGCGGCCGGAGAAGAAGTGTGGAGAATATAATGAAGATGAGGATTTACAAGTTCTATCCGGTACAAACACCGGATACTCCTGACATATCTGAGTTTAAGAATGCATTTGTAAACAGGTACTATGGCACTGCTCATGAGATTCTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

171

Amino Acids

19.97

Weight (kDa)

4.92

Isoelectric Point (pI)

61.84

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018521)

Species Orthologous Gene IDs
prunus_persica Prupe.6G302300_v2.0.a1
pyrus_communis pycom04g16240 pycom12g18350
rosa_multiflora Rmu_co8374625.1_g000001 Rmu_sc0004343.1_g000016 Rmu_sc0006910.1_g000002 Rmu_sc0033812.1_g000003
rosa_roxburghii Rroxscaffold_6G00420570
rosa_rugosa Rorug03G0033000
rosa_samantha Rh3CG094400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 54
AciI CCGC 2 cut(s) 182, 372
AclWI GGATC 2 cut(s) 170, 183
AcoI YGGCCR 1 cut(s) 373
AcuI CTGAAG 1 cut(s) 250
AfaI GTAC 4 cut(s) 227, 263, 432, 488
AgsI TTSAA 3 cut(s) 13, 148, 155
AjnI CCWGG 1 cut(s) 285
AluBI AGCT 2 cut(s) 87, 250
AluI AGCT 2 cut(s) 87, 250
AlwI GGATC 2 cut(s) 170, 183
AoxI GGCC 1 cut(s) 373
ApeKI GCWGC 2 cut(s) 87, 104
AsuHPI GGTGA 2 cut(s) 284, 301
BamHI GGATCC 1 cut(s) 175
BanII GRGCYC 1 cut(s) 28
BarI GAAGNNNNNNTAC 2 cut(s) 140, 172
BbvI GCAGC 2 cut(s) 74, 91
BceAI ACGGC 1 cut(s) 146
BciT130I CCWGG 1 cut(s) 287
BciVI GTATCC 1 cut(s) 436
BfuI GTATCC 1 cut(s) 436
BisI GCNGC 4 cut(s) 88, 105, 183, 373
BlsI GCNGC 4 cut(s) 89, 106, 184, 374
BmcAI AGTACT 1 cut(s) 227
Bme1390I CCNGG 1 cut(s) 287
BmiI GGNNCC 2 cut(s) 27, 177
BmrFI CCNGG 1 cut(s) 287
BmrI ACTGGG 1 cut(s) 31
BmuI ACTGGG 1 cut(s) 31
BpmI CTGGAG 1 cut(s) 272
BpuEI CTTGAG 1 cut(s) 103
BsaWI WCCGGW 2 cut(s) 427, 439
BsaXI ACNNNNNCTCC 6 cut(s) 209, 239, 245, 275, 370, 400
Bse1I ACTGG 1 cut(s) 26
BseBI CCWGG 1 cut(s) 287
BseMII CTCAG 1 cut(s) 450
BseNI ACTGG 1 cut(s) 26
BseRI GAGGAG 3 cut(s) 233, 236, 269
BseX3I CGGCCG 1 cut(s) 373
BseXI GCAGC 2 cut(s) 74, 91
Bsh1285I CGRYCG 1 cut(s) 376
BshFI GGCC 1 cut(s) 375
BsiEI CGRYCG 1 cut(s) 376
BsiSI CCGG 3 cut(s) 376, 428, 440
BsmI GAATGC 1 cut(s) 475
BsnI GGCC 1 cut(s) 375
Bsp1286I GDGCHC 1 cut(s) 28
Bsp143I GATC 3 cut(s) 139, 175, 352
BspACI CCGC 2 cut(s) 182, 372
BspANI GGCC 1 cut(s) 375
BspCNI CTCAG 1 cut(s) 451
BspHI TCATGA 2 cut(s) 199, 502
BspLI GGNNCC 2 cut(s) 27, 177
BspPI GGATC 2 cut(s) 170, 183
BsrI ACTGG 1 cut(s) 26
BssMI GATC 3 cut(s) 139, 175, 352
Bst2UI CCWGG 1 cut(s) 287
BstC8I GCNNGC 1 cut(s) 92
BstDEI CTNAG 1 cut(s) 459
BstKTI GATC 3 cut(s) 142, 178, 355
BstMBI GATC 3 cut(s) 139, 175, 352
BstMCI CGRYCG 1 cut(s) 376
BstNI CCWGG 1 cut(s) 287
BstSCI CCNGG 1 cut(s) 285
BstV1I GCAGC 2 cut(s) 74, 91
BstX2I RGATCY 1 cut(s) 175
BstYI RGATCY 1 cut(s) 175
BstZI CGGCCG 1 cut(s) 373
BsuI GTATCC 1 cut(s) 436
BsuRI GGCC 1 cut(s) 375
BtsI GCAGTG 1 cut(s) 495
BtsIMutI CAGTG 2 cut(s) 274, 495
Cac8I GCNNGC 1 cut(s) 92
CciI TCATGA 2 cut(s) 199, 502
CsiI ACCWGGT 1 cut(s) 285
Csp6I GTAC 4 cut(s) 226, 262, 431, 487
CviAII CATG 3 cut(s) 200, 319, 503
CviJI RGCY 5 cut(s) 26, 87, 104, 250, 375
CviKI_1 RGCY 5 cut(s) 26, 87, 104, 250, 375
CviQI GTAC 4 cut(s) 226, 262, 431, 487
DdeI CTNAG 1 cut(s) 459
DpnI GATC 3 cut(s) 141, 177, 354
DpnII GATC 3 cut(s) 139, 175, 352
EaeI YGGCCR 1 cut(s) 373
EagI CGGCCG 1 cut(s) 373
EclXI CGGCCG 1 cut(s) 373
Eco24I GRGCYC 1 cut(s) 28
Eco52I CGGCCG 1 cut(s) 373
Eco57I CTGAAG 1 cut(s) 250
EcoRII CCWGG 1 cut(s) 285
EcoT22I ATGCAT 1 cut(s) 475
EcoT38I GRGCYC 1 cut(s) 28
FaeI CATG 3 cut(s) 203, 322, 506
FaiI YATR 7 cut(s) 33, 201, 320, 398, 455, 492, 504
FatI CATG 3 cut(s) 199, 318, 502
FblI GTMKAC 1 cut(s) 54
Fnu4HI GCNGC 4 cut(s) 88, 105, 183, 373
FriOI GRGCYC 1 cut(s) 28
Fsp4HI GCNGC 4 cut(s) 88, 105, 183, 373
GluI GCNGC 4 cut(s) 88, 105, 183, 373
GsuI CTGGAG 1 cut(s) 272
HaeIII GGCC 1 cut(s) 375
HapII CCGG 3 cut(s) 376, 428, 440
Hin1II CATG 3 cut(s) 203, 322, 506
HinfI GANTC 1 cut(s) 508
HpaII CCGG 3 cut(s) 376, 428, 440
HphI GGTGA 2 cut(s) 284, 301
Hpy166II GTNNAC 2 cut(s) 55, 481
Hpy188I TCNGA 2 cut(s) 70, 460
Hpy188III TCNNGA 5 cut(s) 82, 200, 215, 449, 503
Hpy8I GTNNAC 2 cut(s) 55, 481
HpyAV CCTTC 3 cut(s) 56, 93, 362
HpyCH4V TGCA 2 cut(s) 90, 473
HpyF3I CTNAG 1 cut(s) 459
Hsp92II CATG 3 cut(s) 203, 322, 506
Kzo9I GATC 3 cut(s) 139, 175, 352
LmnI GCTCC 1 cut(s) 31
Lsp1109I GCAGC 2 cut(s) 74, 91
MabI ACCWGGT 1 cut(s) 285
MaeIII GTNAC 1 cut(s) 272
MalI GATC 3 cut(s) 141, 177, 354
MboI GATC 3 cut(s) 139, 175, 352
MboII GAAGA 8 cut(s) 16, 25, 308, 311, 338, 341, 393, 415
MflI RGATCY 1 cut(s) 175
MhlI GDGCHC 1 cut(s) 28
MnlI CCTC 4 cut(s) 211, 214, 247, 402
Mph1103I ATGCAT 1 cut(s) 475
MseI TTAA 1 cut(s) 465
MslI CAYNNNNRTG 1 cut(s) 120
MspA1I CMGCKG 1 cut(s) 182
MspI CCGG 3 cut(s) 376, 428, 440
MspR9I CCNGG 1 cut(s) 287
Mva1269I GAATGC 1 cut(s) 475
MvaI CCWGG 1 cut(s) 287
NdeII GATC 3 cut(s) 139, 175, 352
NlaIII CATG 3 cut(s) 203, 322, 506
NlaIV GGNNCC 2 cut(s) 27, 177
NmuCI GTSAC 1 cut(s) 272
NsiI ATGCAT 1 cut(s) 475
PagI TCATGA 2 cut(s) 199, 502
PctI GAATGC 1 cut(s) 475
PfeI GAWTC 1 cut(s) 508
PkrI GCNGC 4 cut(s) 89, 106, 184, 374
Psp6I CCWGG 1 cut(s) 285
PspGI CCWGG 1 cut(s) 285
PspN4I GGNNCC 2 cut(s) 27, 177
PsuI RGATCY 1 cut(s) 175
RsaI GTAC 4 cut(s) 227, 263, 432, 488
RsaNI GTAC 4 cut(s) 226, 262, 431, 487
RseI CAYNNNNRTG 1 cut(s) 120
SaqAI TTAA 1 cut(s) 465
SatI GCNGC 4 cut(s) 88, 105, 183, 373
Sau3AI GATC 3 cut(s) 139, 175, 352
ScaI AGTACT 1 cut(s) 227
ScrFI CCNGG 1 cut(s) 287
SduI GDGCHC 1 cut(s) 28
SetI ASST 5 cut(s) 67, 89, 252, 291, 488
SexAI ACCWGGT 1 cut(s) 285
SmiMI CAYNNNNRTG 1 cut(s) 120
SmlI CTYRAG 1 cut(s) 82
SmoI CTYRAG 1 cut(s) 82
SsiI CCGC 2 cut(s) 182, 372
SspI AATATT 1 cut(s) 349
StyD4I CCNGG 1 cut(s) 285
TatI WGTACW 2 cut(s) 225, 261
TauI GCSGC 2 cut(s) 185, 375
TfiI GAWTC 1 cut(s) 508
Tru1I TTAA 1 cut(s) 465
Tru9I TTAA 1 cut(s) 465
TscAI CASTG 2 cut(s) 274, 502
TseFI GTSAC 1 cut(s) 272
TseI GCWGC 2 cut(s) 87, 104
Tsp45I GTSAC 1 cut(s) 272
TspDTI ATGAA 4 cut(s) 17, 216, 321, 416
TspGWI ACGGA 1 cut(s) 18
TspRI CASTG 2 cut(s) 274, 502
XmiI GTMKAC 1 cut(s) 54
ZrmI AGTACT 1 cut(s) 227
Zsp2I ATGCAT 1 cut(s) 475
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.