Rorug03G0046100

Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0015251
Physical Location & Seq
Forward (+)
3553563 .. 3556968
3406 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0046100.1

Sequence Viewer

Length: 1593 bp
ATGGGAAGCCTTCCGTTTACTACCTCTGATCTTCACCCAAGCACCGGCGGATTTGTGAGCAGCCCGACATTGCTATGTACACCCCTAATGGGAACCACAGTGGACCAAATGCTGATTGAGATGCACAAGGCTAAGGAAATTGGTTCTGATATTGTGGAGATCAGGCTTGACTGTTTGAGAAACTTTAACCCAAGTTCAGATCTTCAAATTCTTATCAAGCAGAGTCCTCTGCCTACTCTTGTCACTTACAGGCCAGTTTGGGAAGGCGGTCAATATGAGGGCGATGAAACCAAGCGACAGGATGCTCTTCGGTCAGCAATGGAATTGGGAGCTAACTATATTGATATTGAGCTTGAGGTTGCCCATGACTTCAACAATTCCATCTATGAAAAGAAGCCTGACAACTTCAAAGTCATTGTTTCTTCTCACAACTTTCACAACACTCCATCCTCCGAGGCAATCGGAAATCTTGTGGCTAGAATCCAAGCCACTGGAGCTGACATTGTGAAAATTGCAACTACTGCATTAGACATTACAGATTGTGCCAGAATTTTCCAGATTACCGTGCATTCACAGGTCCCAACAATAGGTATTGTTATGGGAGAGAGAGGTTTGATTTCACGACTACTTAGCCCCAAGTTTGGTGGATATCTTACCTATGGTGCACTAGAAGCCGGTGCTATATCAGCTCCTGGGCAACCAACTGCGAGAGACTTGCTGGATTTATACAACTTCCGACTCATTAGACCTGATACCAAAGTATATGGGATTATTGGGAAGCCTGTTGGTCACAGCAAGAGTCCTCTACTATTCAATGCAGCATTCAAGTCAGTTGGCCTTAATGCAGTTTATGTTCATTTTCTGGTGGATGATGTCGAGAAGTTTTTCAACACATATTCATCCGTTGATTTTTCGGGATGCAGTTGTACTATTCCACATAAGGAGGCAGCACTCAAATGCATGGATGAGATTGATTCCATTGCCAAGAAAATTGGAGCTATCAACAACATTGTCAGGAAGCCTGATGGGAGGTTAGTTGCTTTCAACACCGACTACATTGGCTCCATCAGTGGCATTGAGGACGGACTACGAGGTTTGAACGGCGCCATTCCTGCTGGTAAATCACCCCTGGCTGGAAAGTTGTTTGTGGTCTTGGGAGCCGGTGGTGCTGGCAAGTCACTTGTGTATGGTGCTGCACAGAAAGGAGCAAGAGTTGTATGTGCCAACCGAACATATGAAAGAGCTAAGGAGCTGGCTGACAAAGTTGGGGGAAAAGCTATGACTCTTGAAGAAGTTGAAAATTTCCACCCGGAAGAGGGAATGATTCTTGCCAACACTACATCTGTGGGAATGAAGCCTAATGTTGATGATACCCCTATCTCCAAGCAAGCTCTGAAACACTATTGCTTAGTTTTCGATGCTATCTATACACCCAAAGAGACTAGACTCCTCCGGGAAGCAAAAGAGACTGGAGCAGAGGTTGTATATGGGACAGAGATGTTGATCAGGCAAGGGTTTGAACAATACAAGAACTTCACTGGTTTGCCAGCACCTGAAGAATTGTTTAGGGAGCTCATGGAGAAGCATGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005215 GO:0006810 GO:0006855 GO:0008150 GO:0015238 GO:0015893 GO:0022857 GO:0042221 GO:0042493 GO:0050896 GO:0051179 GO:0051234 GO:0055085
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MatE

Protein Analysis

530

Amino Acids

57.67

Weight (kDa)

6.0

Isoelectric Point (pI)

29.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DHquinase_I PF01487 26 - 243 1.6e-68 Type I 3-dehydroquinase
Shikimate_dh_N PF08501 257 - 337 9.9e-25 Shikimate dehydrogenase substrate binding domain
Shikimate_DH PF01488 377 - 448 4.6e-08 Shikimate / quinate 5-dehydrogenase
SDH_C PF18317 497 - 526 4.7e-08 Shikimate 5'-dehydrogenase C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015251)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G38510
fragaria_vesca FvH4_6g10060
malus_domestica MD04G1173000.v1.1 MD12G1186300.v1.1
prunus_persica Prupe.6G291800_v2.0.a1
pyrus_communis pycom12g17490
rosa_chinensis RchiOBHm_Chr3g0460761
rosa_laevigata RLG00000024983
rosa_multiflora Rmu_sc0007678.1_g000022
rosa_roxburghii Rroxscaffold_6G00419240
rosa_rugosa Rorug03G0046100
rosa_samantha Rh3AG103300 Rh3BG106900 Rh3CG108800 Rh3DG108600
rosa_wichuraiana Rw3G008790

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1103
AciI CCGC 2 cut(s) 48, 267
AcsI RAATTY 3 cut(s) 207, 549, 1300
AcuI CTGAAG 1 cut(s) 1575
AcyI GRCGYC 1 cut(s) 1104
AfaI GTAC 2 cut(s) 79, 928
AfiI CCNNNNNNNGG 7 cut(s) 44, 89, 587, 641, 1133, 1315, 1316
AjnI CCWGG 2 cut(s) 691, 1128
Alw21I GWGCWC 2 cut(s) 667, 1575
Alw26I GTCTC 3 cut(s) 705, 1433, 1460
Alw44I GTGCAC 1 cut(s) 663
AlwNI CAGNNNCTG 2 cut(s) 692, 1553
AoxI GGCC 2 cut(s) 251, 835
ApaLI GTGCAC 1 cut(s) 663
ApeKI GCWGC 4 cut(s) 60, 818, 947, 1193
ApoI RAATTY 3 cut(s) 207, 549, 1300
Asp700I GAANNNNTTC 1 cut(s) 884
AspLEI GCGC 1 cut(s) 1106
AspS9I GGNCC 2 cut(s) 103, 577
AsuC2I CCSGG 2 cut(s) 1310, 1454
AsuHPI GGTGA 2 cut(s) 26, 1116
AvaII GGWCC 2 cut(s) 103, 577
BaeGI GKGCMC 1 cut(s) 667
BaeI ACNNNNGTAYC 2 cut(s) 744, 777
BanI GGYRCC 1 cut(s) 1103
BanII GRGCYC 1 cut(s) 1575
Bbv12I GWGCWC 2 cut(s) 667, 1575
BbvI GCAGC 4 cut(s) 72, 830, 959, 1180
BccI CCATC 4 cut(s) 389, 454, 1019, 1073
BceAI ACGGC 1 cut(s) 1117
BcgI CGANNNNNNTGC 2 cut(s) 697, 731
BciT130I CCWGG 2 cut(s) 693, 1130
BclI TGATCA 1 cut(s) 1503
BcnI CCSGG 2 cut(s) 1310, 1454
BcoDI GTCTC 3 cut(s) 705, 1433, 1460
BfaI CTAG 3 cut(s) 477, 668, 1443
BfoI RGCGCY 1 cut(s) 1107
BglII AGATCT 1 cut(s) 199
BisI GCNGC 4 cut(s) 61, 819, 948, 1194
BlsI GCNGC 4 cut(s) 62, 820, 949, 1195
Bme1390I CCNGG 4 cut(s) 693, 1130, 1310, 1454
Bme18I GGWCC 2 cut(s) 103, 577
BmgT120I GGNCC 2 cut(s) 103, 577
BmiI GGNNCC 5 cut(s) 94, 579, 1063, 1105, 1159
BmrFI CCNGG 4 cut(s) 693, 1130, 1310, 1454
BmsI GCATC 4 cut(s) 111, 292, 908, 1408
BpmI CTGGAG 2 cut(s) 513, 1491
Bpu10I CCTNAGC 2 cut(s) 132, 1245
BpuEI CTTGAG 1 cut(s) 374
BpuMI CCSGG 2 cut(s) 1310, 1454
BsaBI GATNNNNATC 1 cut(s) 1502
BsaHI GRCGYC 1 cut(s) 1104
BsaJI CCNNGG 3 cut(s) 453, 692, 1128
BsaXI ACNNNNNCTCC 6 cut(s) 1046, 1076, 1197, 1227, 1464, 1494
Bsc4I CCNNNNNNNGG 7 cut(s) 44, 89, 587, 641, 1133, 1315, 1316
Bse118I RCCGGY 3 cut(s) 44, 674, 1160
Bse1I ACTGG 4 cut(s) 254, 496, 1474, 1543
Bse3DI GCAATG 3 cut(s) 68, 324, 978
Bse8I GATNNNNATC 1 cut(s) 1502
BseBI CCWGG 2 cut(s) 693, 1130
BseDI CCNNGG 3 cut(s) 453, 692, 1128
BseGI GGATG 6 cut(s) 307, 446, 874, 899, 923, 970
BseJI GATNNNNATC 1 cut(s) 1502
BseLI CCNNNNNNNGG 7 cut(s) 44, 89, 587, 641, 1133, 1315, 1316
BseMI GCAATG 3 cut(s) 68, 324, 978
BseNI ACTGG 4 cut(s) 254, 496, 1474, 1543
BseRI GAGGAG 1 cut(s) 1439
BseSI GKGCMC 1 cut(s) 667
BseXI GCAGC 4 cut(s) 72, 830, 959, 1180
BsgI GTGCAG 1 cut(s) 1179
BshFI GGCC 2 cut(s) 253, 837
BshNI GGYRCC 1 cut(s) 1103
BsiHKAI GWGCWC 2 cut(s) 667, 1575
BsiSI CCGG 5 cut(s) 45, 675, 1161, 1310, 1453
BslFI GGGAC 2 cut(s) 563, 1504
BslI CCNNNNNNNGG 7 cut(s) 44, 89, 587, 641, 1133, 1315, 1316
BsmAI GTCTC 3 cut(s) 705, 1433, 1460
BsmFI GGGAC 2 cut(s) 563, 1504
BsmI GAATGC 2 cut(s) 568, 821
BsnI GGCC 2 cut(s) 253, 837
Bsp1286I GDGCHC 2 cut(s) 667, 1575
Bsp1407I TGTACA 1 cut(s) 77
Bsp143I GATC 4 cut(s) 28, 159, 199, 1503
BspACI CCGC 2 cut(s) 48, 267
BspANI GGCC 2 cut(s) 253, 837
BspLI GGNNCC 5 cut(s) 94, 579, 1063, 1105, 1159
BspQI GCTCTTC 1 cut(s) 312
BspT107I GGYRCC 1 cut(s) 1103
BsrDI GCAATG 3 cut(s) 68, 324, 978
BsrFI RCCGGY 3 cut(s) 44, 674, 1160
BsrGI TGTACA 1 cut(s) 77
BsrI ACTGG 4 cut(s) 254, 496, 1474, 1543
BssAI RCCGGY 3 cut(s) 44, 674, 1160
BssECI CCNNGG 3 cut(s) 453, 692, 1128
BssMI GATC 4 cut(s) 28, 159, 199, 1503
BssNI GRCGYC 1 cut(s) 1104
Bst2UI CCWGG 2 cut(s) 693, 1130
Bst4CI ACNGT 3 cut(s) 100, 173, 565
Bst6I CTCTTC 2 cut(s) 312, 1308
BstACI GRCGYC 1 cut(s) 1104
BstAPI GCANNNNNTGC 1 cut(s) 521
BstAUI TGTACA 1 cut(s) 77
BstC8I GCNNGC 5 cut(s) 1171, 1254, 1389, 1548, 1587
BstDEI CTNAG 4 cut(s) 132, 629, 1245, 1408
BstF5I GGATG 6 cut(s) 307, 446, 874, 899, 923, 970
BstH2I RGCGCY 1 cut(s) 1107
BstHHI GCGC 1 cut(s) 1106
BstKTI GATC 4 cut(s) 31, 162, 202, 1506
BstMAI GTCTC 3 cut(s) 705, 1433, 1460
BstMBI GATC 4 cut(s) 28, 159, 199, 1503
BstMWI GCNNNNNNNGC 6 cut(s) 494, 521, 671, 686, 1112, 1166
BstNI CCWGG 2 cut(s) 693, 1130
BstNSI RCATGY 1 cut(s) 1589
BstSCI CCNGG 4 cut(s) 691, 1128, 1308, 1452
BstSLI GKGCMC 1 cut(s) 667
BstV1I GCAGC 4 cut(s) 72, 830, 959, 1180
BstX2I RGATCY 1 cut(s) 199
BstXI CCANNNNNNTGG 1 cut(s) 491
BstYI RGATCY 1 cut(s) 199
BsuRI GGCC 2 cut(s) 253, 837
BtgZI GCGATG 1 cut(s) 297
BtsCI GGATG 6 cut(s) 307, 446, 874, 899, 923, 970
BtsIMutI CAGTG 4 cut(s) 105, 489, 1075, 1536
Cac8I GCNNGC 5 cut(s) 1171, 1254, 1389, 1548, 1587
CaiI CAGNNNCTG 2 cut(s) 692, 1553
CfoI GCGC 1 cut(s) 1106
Cfr10I RCCGGY 3 cut(s) 44, 674, 1160
Cfr13I GGNCC 2 cut(s) 103, 577
Csp6I GTAC 2 cut(s) 78, 927
CspCI CAANNNNNGTGG 2 cut(s) 625, 660
CviAII CATG 4 cut(s) 365, 961, 1576, 1586
CviQI GTAC 2 cut(s) 78, 927
DdeI CTNAG 4 cut(s) 132, 629, 1245, 1408
DinI GGCGCC 1 cut(s) 1105
DpnI GATC 4 cut(s) 30, 161, 201, 1505
DpnII GATC 4 cut(s) 28, 159, 199, 1503
Eam1104I CTCTTC 2 cut(s) 312, 1308
EarI CTCTTC 2 cut(s) 312, 1308
EciI GGCGGA 1 cut(s) 63
Ecl136II GAGCTC 1 cut(s) 1573
Eco24I GRGCYC 1 cut(s) 1575
Eco32I GATATC 1 cut(s) 650
Eco47I GGWCC 2 cut(s) 103, 577
Eco53kI GAGCTC 1 cut(s) 1573
Eco57I CTGAAG 1 cut(s) 1575
EcoICRI GAGCTC 1 cut(s) 1573
EcoO109I RGGNCCY 1 cut(s) 577
EcoRII CCWGG 2 cut(s) 691, 1128
EcoRV GATATC 1 cut(s) 650
EcoT22I ATGCAT 2 cut(s) 962, 1591
EcoT38I GRGCYC 1 cut(s) 1575
EgeI GGCGCC 1 cut(s) 1105
EheI GGCGCC 1 cut(s) 1105
FaeI CATG 4 cut(s) 368, 964, 1579, 1589
FaqI GGGAC 2 cut(s) 563, 1504
FatI CATG 4 cut(s) 364, 960, 1575, 1585
FauNDI CATATG 1 cut(s) 1234
FbaI TGATCA 1 cut(s) 1503
Fnu4HI GCNGC 4 cut(s) 61, 819, 948, 1194
FokI GGATG 6 cut(s) 314, 433, 881, 886, 930, 977
FriOI GRGCYC 1 cut(s) 1575
Fsp4HI GCNGC 4 cut(s) 61, 819, 948, 1194
FspBI CTAG 3 cut(s) 477, 668, 1443
GlaI GCGC 1 cut(s) 1105
GluI GCNGC 4 cut(s) 61, 819, 948, 1194
GsuI CTGGAG 2 cut(s) 513, 1491
HaeII RGCGCY 1 cut(s) 1107
HaeIII GGCC 2 cut(s) 253, 837
HapII CCGG 5 cut(s) 45, 675, 1161, 1310, 1453
HhaI GCGC 1 cut(s) 1106
Hin1I GRCGYC 1 cut(s) 1104
Hin1II CATG 4 cut(s) 368, 964, 1579, 1589
Hin6I GCGC 1 cut(s) 1104
HinP1I GCGC 1 cut(s) 1104
HinfI GANTC 8 cut(s) 223, 480, 738, 799, 974, 1282, 1324, 1446
HpaII CCGG 5 cut(s) 45, 675, 1161, 1310, 1453
HphI GGTGA 2 cut(s) 26, 1116
Hpy166II GTNNAC 4 cut(s) 18, 80, 103, 665
Hpy188I TCNGA 7 cut(s) 28, 148, 199, 454, 464, 737, 1395
Hpy188III TCNNGA 6 cut(s) 556, 621, 877, 915, 1015, 1286
Hpy8I GTNNAC 4 cut(s) 18, 80, 103, 665
HpyAV CCTTC 2 cut(s) 20, 257
HpyCH4III ACNGT 3 cut(s) 100, 173, 565
HpyF10VI GCNNNNNNNGC 6 cut(s) 494, 521, 671, 686, 1112, 1166
HpyF3I CTNAG 4 cut(s) 132, 629, 1245, 1408
Hsp92I GRCGYC 1 cut(s) 1104
Hsp92II CATG 4 cut(s) 368, 964, 1579, 1589
HspAI GCGC 1 cut(s) 1104
KasI GGCGCC 1 cut(s) 1103
Ksp22I TGATCA 1 cut(s) 1503
Kzo9I GATC 4 cut(s) 28, 159, 199, 1503
LguI GCTCTTC 1 cut(s) 312
Lsp1109I GCAGC 4 cut(s) 72, 830, 959, 1180
LweI GCATC 4 cut(s) 111, 292, 908, 1408
MaeI CTAG 3 cut(s) 477, 668, 1443
MaeIII GTNAC 3 cut(s) 241, 788, 1176
MalI GATC 4 cut(s) 30, 161, 201, 1505
MboI GATC 4 cut(s) 28, 159, 199, 1503
MboII GAAGA 7 cut(s) 23, 194, 299, 414, 1301, 1325, 1568
MflI RGATCY 1 cut(s) 199
MhlI GDGCHC 2 cut(s) 667, 1575
MluCI AATT 9 cut(s) 138, 207, 323, 376, 510, 549, 990, 1300, 1559
Mly113I GGCGCC 1 cut(s) 1104
MlyI GAGTC 5 cut(s) 232, 732, 808, 1276, 1440
MmeI TCCRAC 1 cut(s) 760
Mph1103I ATGCAT 2 cut(s) 962, 1591
MroXI GAANNNNTTC 1 cut(s) 884
MseI TTAA 2 cut(s) 186, 840
MslI CAYNNNNRTG 2 cut(s) 73, 955
MspI CCGG 5 cut(s) 45, 675, 1161, 1310, 1453
MspR9I CCNGG 4 cut(s) 693, 1130, 1310, 1454
Mva1269I GAATGC 2 cut(s) 568, 821
MvaI CCWGG 2 cut(s) 693, 1130
MwoI GCNNNNNNNGC 6 cut(s) 494, 521, 671, 686, 1112, 1166
NarI GGCGCC 1 cut(s) 1104
NciI CCSGG 2 cut(s) 1310, 1454
NdeI CATATG 1 cut(s) 1234
NdeII GATC 4 cut(s) 28, 159, 199, 1503
NlaIII CATG 4 cut(s) 368, 964, 1579, 1589
NlaIV GGNNCC 5 cut(s) 94, 579, 1063, 1105, 1159
NmuCI GTSAC 3 cut(s) 241, 788, 1176
NsiI ATGCAT 2 cut(s) 962, 1591
NspI RCATGY 1 cut(s) 1589
PaeI GCATGC 1 cut(s) 1589
PciSI GCTCTTC 1 cut(s) 312
PctI GAATGC 2 cut(s) 568, 821
PdmI GAANNNNTTC 1 cut(s) 884
PfeI GAWTC 3 cut(s) 480, 974, 1324
PfoI TCCNGGA 1 cut(s) 1452
PkrI GCNGC 4 cut(s) 62, 820, 949, 1195
PleI GAGTC 5 cut(s) 231, 732, 807, 1276, 1440
PluTI GGCGCC 1 cut(s) 1107
PpsI GAGTC 5 cut(s) 231, 732, 807, 1276, 1440
PpuMI RGGWCCY 1 cut(s) 577
Psp124BI GAGCTC 1 cut(s) 1575
Psp5II RGGWCCY 1 cut(s) 577
Psp6I CCWGG 2 cut(s) 691, 1128
PspGI CCWGG 2 cut(s) 691, 1128
PspN4I GGNNCC 5 cut(s) 94, 579, 1063, 1105, 1159
PspPI GGNCC 2 cut(s) 103, 577
PspPPI RGGWCCY 1 cut(s) 577
PstNI CAGNNNCTG 2 cut(s) 692, 1553
PsuI RGATCY 1 cut(s) 199
RsaI GTAC 2 cut(s) 79, 928
RsaNI GTAC 2 cut(s) 78, 927
RseI CAYNNNNRTG 2 cut(s) 73, 955
SacI GAGCTC 1 cut(s) 1575
SapI GCTCTTC 1 cut(s) 312
SaqAI TTAA 2 cut(s) 186, 840
SatI GCNGC 4 cut(s) 61, 819, 948, 1194
Sau3AI GATC 4 cut(s) 28, 159, 199, 1503
Sau96I GGNCC 2 cut(s) 103, 577
SchI GAGTC 5 cut(s) 232, 732, 808, 1276, 1440
ScrFI CCNGG 4 cut(s) 693, 1130, 1310, 1454
SduI GDGCHC 2 cut(s) 667, 1575
SfaNI GCATC 4 cut(s) 111, 292, 908, 1408
SfoI GGCGCC 1 cut(s) 1105
SgrAI CRCCGGYG 1 cut(s) 44
SinI GGWCC 2 cut(s) 103, 577
SmiMI CAYNNNNRTG 2 cut(s) 73, 955
SmlI CTYRAG 1 cut(s) 353
SmoI CTYRAG 1 cut(s) 353
SphI GCATGC 1 cut(s) 1589
Sse9I AATT 9 cut(s) 138, 207, 323, 376, 510, 549, 990, 1300, 1559
SsiI CCGC 2 cut(s) 48, 267
SspDI GGCGCC 1 cut(s) 1103
SspMI CTAG 3 cut(s) 477, 668, 1443
SstI GAGCTC 1 cut(s) 1575
StyD4I CCNGG 4 cut(s) 691, 1128, 1308, 1452
TaaI ACNGT 3 cut(s) 100, 173, 565
TaqI TCGA 2 cut(s) 876, 1416
TaqII GACCGA 1 cut(s) 300
TasI AATT 9 cut(s) 138, 207, 323, 376, 510, 549, 990, 1300, 1559
TatI WGTACW 2 cut(s) 77, 926
TfiI GAWTC 3 cut(s) 480, 974, 1324
Tru1I TTAA 2 cut(s) 186, 840
Tru9I TTAA 2 cut(s) 186, 840
TscAI CASTG 4 cut(s) 105, 496, 1075, 1543
TseFI GTSAC 3 cut(s) 241, 788, 1176
TseI GCWGC 4 cut(s) 60, 818, 947, 1193
Tsp45I GTSAC 3 cut(s) 241, 788, 1176
TspDTI ATGAA 6 cut(s) 300, 402, 845, 888, 1251, 1367
TspGWI ACGGA 2 cut(s) 892, 1098
TspRI CASTG 4 cut(s) 105, 496, 1075, 1543
VneI GTGCAC 1 cut(s) 663
VpaK11BI GGWCC 2 cut(s) 103, 577
XapI RAATTY 3 cut(s) 207, 549, 1300
XceI RCATGY 1 cut(s) 1589
XmnI GAANNNNTTC 1 cut(s) 884
XspI CTAG 3 cut(s) 477, 668, 1443
Zsp2I ATGCAT 2 cut(s) 962, 1591
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.