Rh3DG108600

Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3D
OG0015251
Physical Location & Seq
Reverse (-)
8754418 .. 8757891
3474 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3DG108600.1

Sequence Viewer

Length: 1653 bp
ATGTGCCTTCCTGGTACTGAATCTCGAGGAATTATAAATGGTCACGACCCTCTGATTCCGAAGCTAGATGGAGGAGGAGACTATAACAATGAAGAAGAGTACATGACAATGAGGTCAGATAGAAGCGAAGGTGGCCACAGCAGCAGCACTAGCTTCATACAAAACCTCCGTCAACGGCTACAGCAGCAGCTCCCCACAAATGGGCTTCTCGGACGGCTGCCTCTAACCGAGATAGGAGAAGAGTTACGGTCGCTGATAAAGATTGGAGGTCCAATAGTGATAACATCCCTACTTATATATTCAAGGTCTGTAATTTCAATGCTCTTCTTAGGTCGTCTTGGAAAAGCAGAGTTGGCCGGAGGCTCATTAGCACTTGGGTTTGGAAATATTACAGGCATCTCAATCCTCAGAGGGCTATGTACTGGTATGGACCCAATCTGTTGCCAAGCTTATGGAGCTCAAAGATGGACAGTTCTCAGCCAAACCTTCCAGAAAACCCTTTGTCTCCTTCTATTAGTTTCCATACCCATCTCATTCGCGTGGCTCAATATTGAGCCAATTTTCCTATGGTTAGGTCAAGACCCACAGATTACAAGAGTTGCTAGGGTTTACATGGTTTGCTCAATCCCTGAATTGCTAGCTCAAGCTCACCTCAACCCATTGAGAGTCTTCTTAAGAACACAAGGCCTTACCGCCCCACTTACTTTAGCTGCATGTTTCTCGGCCCTGATGCACTTACCCATCAACTATTTTTTAGTCTCTTACTTGAATTTGGGGGTGCAAGGTGTTGCTTTGGGCCTTGCTTGGAACACTTGGAATTTGAATGTGGGATTGGTGATTTATCTAGCTATCTCAAATAGAGCATTGAAACCTTGGAGTGGGCTCACATTTGTCTCTACGTTTCAAGGATGGAGACCGTTGCTAAGTTTAGCACTACCCAGTTGCGTTTCAGTGTGCTTGGAATGGTGGTGGTATGAAATTATGTTGTTCCTCTGTGGCATTTTGAGTAACCCACAAGCTAGTGTTGCAGCAATGGGTATCCTAATTCAAACAACAGGAATGTTATACATCTTTCCAATTTCGTTAAGTGCCGCCTTGTCAACTAGGGTTGGACATTCCTTGGGGGCGGGGCAACCCAGCCGAGCCCGATGGGCTGCCCTAATCGGTCTTCTTGTCGCGTTTGCTTTTGGGCTCTTCGCATTTGTTTTCATGACTTCTGTGAGAAATGTGTGGGGGACTATGTTCACAAATGAACCACAAATTCTTGAACTGATTTCAACGGCACTTCCAATCTTGGGGCTATGTGAACTTGGCAACACTCCTCAAACAGCTGCATGTGGAGTCTTGACAGGTACTGCACGACCTAAGCTTGGAGCGAGAATTAACTTGTATTCATTTTACCTTATTGGATTGCCAGTTGCTATTATTGCAACTTTTACATATGAAGTTGGGTTTTTGGGGCTGTGGCTTGGGTTACTATCAGCACAGGCGTCTTGTGTGTGTATGATGGTGTACACACTGGTTCACACAGATTGGAAGCACCAAACTAGAAGAGCTGAGGAGCTTACTCTGGCTGTGGTAGAGACTAATATTGATGAGAAAGATGATGAAGAAAGTGGCCCGCTAACTCCTTCTATCTCTGAGCTGACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005215 GO:0006810 GO:0006855 GO:0008150 GO:0015238 GO:0015893 GO:0022857 GO:0042221 GO:0042493 GO:0050896 GO:0051179 GO:0051234 GO:0055085
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MatE

Protein Analysis

550

Amino Acids

60.04

Weight (kDa)

6.66

Isoelectric Point (pI)

43.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MatE PF01554 91 - 251 2.8e-35 MatE
MatE PF01554 313 - 476 6.6e-35 MatE
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015251)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G38510
fragaria_vesca FvH4_6g10060
malus_domestica MD04G1173000.v1.1 MD12G1186300.v1.1
prunus_persica Prupe.6G291800_v2.0.a1
pyrus_communis pycom12g17490
rosa_chinensis RchiOBHm_Chr3g0460761
rosa_laevigata RLG00000024983
rosa_multiflora Rmu_sc0007678.1_g000022
rosa_roxburghii Rroxscaffold_6G00419240
rosa_rugosa Rorug03G0046100
rosa_samantha Rh3AG103300 Rh3BG106900 Rh3CG108800 Rh3DG108600
rosa_wichuraiana Rw3G008790

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 35
AasI GACNNNNNNGTC 1 cut(s) 112
AccII CGCG 2 cut(s) 539, 1178
AciI CCGC 4 cut(s) 693, 1092, 1127, 1622
AcoI YGGCCR 2 cut(s) 133, 354
AcsI RAATTY 3 cut(s) 769, 817, 1260
AcyI GRCGYC 1 cut(s) 1490
AfaI GTAC 5 cut(s) 16, 101, 421, 1354, 1514
AfiI CCNNNNNNNGG 5 cut(s) 200, 201, 878, 1295, 1370
AflII CTTAAG 1 cut(s) 673
AgsI TTSAA 9 cut(s) 303, 318, 769, 823, 868, 905, 1049, 1268, 1278
AjnI CCWGG 1 cut(s) 10
AjuI GAANNNNNNNTTGG 2 cut(s) 815, 847
Alw21I GWGCWC 1 cut(s) 460
Alw26I GTCTC 6 cut(s) 72, 509, 763, 898, 907, 1577
AlwNI CAGNNNCTG 1 cut(s) 1355
Ama87I CYCGRG 1 cut(s) 24
AoxI GGCC 6 cut(s) 133, 354, 685, 723, 796, 1618
ApeKI GCWGC 9 cut(s) 141, 144, 184, 187, 217, 710, 1028, 1154, 1331
ApoI RAATTY 3 cut(s) 769, 817, 1260
ArsI GACNNNNNNTTYG 2 cut(s) 154, 186
AspS9I GGNCC 5 cut(s) 269, 430, 724, 796, 1619
AsuHPI GGTGA 2 cut(s) 641, 847
AsuNHI GCTAGC 1 cut(s) 637
AvaI CYCGRG 1 cut(s) 24
AvaII GGWCC 2 cut(s) 269, 430
BalI TGGCCA 1 cut(s) 135
BanII GRGCYC 4 cut(s) 460, 885, 1147, 1194
BbsI GAAGAC 2 cut(s) 661, 1160
Bbv12I GWGCWC 1 cut(s) 460
BbvCI CCTCAGC 1 cut(s) 1557
BbvI GCAGC 9 cut(s) 153, 156, 196, 199, 204, 697, 1040, 1141, 1318
BccI CCATC 7 cut(s) 62, 459, 536, 749, 903, 1143, 1501
BceAI ACGGC 3 cut(s) 191, 230, 1296
BcgI CGANNNNNNTGC 2 cut(s) 702, 736
BciT130I CCWGG 1 cut(s) 12
BciVI GTATCC 1 cut(s) 1049
BcoDI GTCTC 6 cut(s) 72, 509, 763, 898, 907, 1577
BfaI CTAG 8 cut(s) 65, 150, 603, 638, 845, 1020, 1104, 1548
BfmI CTRYAG 1 cut(s) 179
BfrI CTTAAG 1 cut(s) 673
BfuI GTATCC 1 cut(s) 1049
BglI GCCNNNNNGGC 1 cut(s) 1151
Bme1390I CCNGG 1 cut(s) 12
Bme18I GGWCC 2 cut(s) 269, 430
BmeT110I CYCGRG 1 cut(s) 24
BmgT120I GGNCC 5 cut(s) 269, 430, 724, 796, 1619
BmiI GGNNCC 1 cut(s) 432
BmrFI CCNGG 1 cut(s) 12
BmrI ACTGGG 1 cut(s) 933
BmsI GCATC 2 cut(s) 405, 720
BmtI GCTAGC 1 cut(s) 641
BmuI ACTGGG 1 cut(s) 933
BpiI GAAGAC 2 cut(s) 661, 1160
Bpu10I CCTNAGC 2 cut(s) 1365, 1557
BpuEI CTTGAG 1 cut(s) 627
BsaHI GRCGYC 1 cut(s) 1490
BsaI GGTCTC 1 cut(s) 907
BsaJI CCNNGG 2 cut(s) 872, 1119
BsaXI ACNNNNNCTCC 2 cut(s) 150, 180
Bsc4I CCNNNNNNNGG 5 cut(s) 200, 201, 878, 1295, 1370
Bse1I ACTGG 4 cut(s) 427, 939, 1415, 1524
Bse3DI GCAATG 1 cut(s) 1038
BseBI CCWGG 1 cut(s) 12
BseDI CCNNGG 2 cut(s) 872, 1119
BseGI GGATG 2 cut(s) 284, 914
BseLI CCNNNNNNNGG 5 cut(s) 200, 201, 878, 1295, 1370
BseMI GCAATG 1 cut(s) 1038
BseMII CTCAG 4 cut(s) 421, 490, 1548, 1632
BseNI ACTGG 4 cut(s) 427, 939, 1415, 1524
BseRI GAGGAG 4 cut(s) 87, 90, 1311, 1574
BseXI GCAGC 9 cut(s) 153, 156, 196, 199, 204, 697, 1040, 1141, 1318
BseYI CCCAGC 1 cut(s) 1136
BsgI GTGCAG 1 cut(s) 1341
Bsh1236I CGCG 2 cut(s) 539, 1178
Bsh1285I CGRYCG 1 cut(s) 251
BshFI GGCC 6 cut(s) 135, 356, 687, 725, 798, 1620
BsiEI CGRYCG 1 cut(s) 251
BsiHKAI GWGCWC 1 cut(s) 460
BsiHKCI CYCGRG 1 cut(s) 24
BsiSI CCGG 1 cut(s) 357
BslFI GGGAC 1 cut(s) 1249
BslI CCNNNNNNNGG 5 cut(s) 200, 201, 878, 1295, 1370
BsmAI GTCTC 6 cut(s) 72, 509, 763, 898, 907, 1577
BsmFI GGGAC 1 cut(s) 1249
BsnI GGCC 6 cut(s) 135, 356, 687, 725, 798, 1620
Bso31I GGTCTC 1 cut(s) 907
BsoBI CYCGRG 1 cut(s) 24
Bsp1286I GDGCHC 4 cut(s) 460, 885, 1147, 1194
Bsp1407I TGTACA 1 cut(s) 1512
BspACI CCGC 4 cut(s) 693, 1092, 1127, 1622
BspANI GGCC 6 cut(s) 135, 356, 687, 725, 798, 1620
BspCNI CTCAG 4 cut(s) 420, 489, 1549, 1633
BspFNI CGCG 2 cut(s) 539, 1178
BspHI TCATGA 1 cut(s) 1209
BspLI GGNNCC 1 cut(s) 432
BspOI GCTAGC 1 cut(s) 641
BspQI GCTCTTC 3 cut(s) 329, 1199, 1546
BspTI CTTAAG 1 cut(s) 673
BspTNI GGTCTC 1 cut(s) 907
BsrDI GCAATG 1 cut(s) 1038
BsrGI TGTACA 1 cut(s) 1512
BsrI ACTGG 4 cut(s) 427, 939, 1415, 1524
BssECI CCNNGG 2 cut(s) 872, 1119
BssNI GRCGYC 1 cut(s) 1490
BssT1I CCWWGG 2 cut(s) 872, 1119
Bst2UI CCWGG 1 cut(s) 12
Bst4CI ACNGT 3 cut(s) 249, 472, 918
Bst6I CTCTTC 5 cut(s) 90, 234, 329, 1199, 1546
BstACI GRCGYC 1 cut(s) 1490
BstAFI CTTAAG 1 cut(s) 673
BstAUI TGTACA 1 cut(s) 1512
BstC8I GCNNGC 2 cut(s) 639, 1622
BstDEI CTNAG 7 cut(s) 328, 407, 476, 923, 1365, 1557, 1641
BstF5I GGATG 2 cut(s) 284, 914
BstFNI CGCG 2 cut(s) 539, 1178
BstMAI GTCTC 6 cut(s) 72, 509, 763, 898, 907, 1577
BstMCI CGRYCG 1 cut(s) 251
BstMWI GCNNNNNNNGC 9 cut(s) 132, 141, 150, 184, 353, 455, 1025, 1151, 1427
BstNI CCWGG 1 cut(s) 12
BstNSI RCATGY 2 cut(s) 717, 1338
BstSCI CCNGG 1 cut(s) 10
BstSFI CTRYAG 1 cut(s) 179
BstUI CGCG 2 cut(s) 539, 1178
BstV1I GCAGC 9 cut(s) 153, 156, 196, 199, 204, 697, 1040, 1141, 1318
BstV2I GAAGAC 2 cut(s) 661, 1160
BstXI CCANNNNNNTGG 1 cut(s) 452
BsuI GTATCC 1 cut(s) 1049
BsuRI GGCC 6 cut(s) 135, 356, 687, 725, 798, 1620
BtsCI GGATG 2 cut(s) 284, 914
BtsIMutI CAGTG 2 cut(s) 957, 1517
Cac8I GCNNGC 2 cut(s) 639, 1622
CaiI CAGNNNCTG 1 cut(s) 1355
CciI TCATGA 1 cut(s) 1209
Cfr13I GGNCC 5 cut(s) 269, 430, 724, 796, 1619
CseI GACGC 1 cut(s) 1479
Csp6I GTAC 5 cut(s) 15, 100, 420, 1353, 1513
CviAII CATG 6 cut(s) 103, 613, 714, 1210, 1335, 1650
CviQI GTAC 5 cut(s) 15, 100, 420, 1353, 1513
DdeI CTNAG 7 cut(s) 328, 407, 476, 923, 1365, 1557, 1641
DrdI GACNNNNNNGTC 1 cut(s) 112
DseDI GACNNNNNNGTC 1 cut(s) 112
EaeI YGGCCR 2 cut(s) 133, 354
Eam1104I CTCTTC 5 cut(s) 90, 234, 329, 1199, 1546
EarI CTCTTC 5 cut(s) 90, 234, 329, 1199, 1546
Ecl136II GAGCTC 1 cut(s) 458
Eco130I CCWWGG 2 cut(s) 872, 1119
Eco147I AGGCCT 1 cut(s) 687
Eco24I GRGCYC 4 cut(s) 460, 885, 1147, 1194
Eco31I GGTCTC 1 cut(s) 907
Eco47I GGWCC 2 cut(s) 269, 430
Eco53kI GAGCTC 1 cut(s) 458
Eco88I CYCGRG 1 cut(s) 24
EcoICRI GAGCTC 1 cut(s) 458
EcoRII CCWGG 1 cut(s) 10
EcoT14I CCWWGG 2 cut(s) 872, 1119
EcoT38I GRGCYC 4 cut(s) 460, 885, 1147, 1194
ErhI CCWWGG 2 cut(s) 872, 1119
FaeI CATG 6 cut(s) 106, 616, 717, 1213, 1338, 1653
FaqI GGGAC 1 cut(s) 1249
FatI CATG 6 cut(s) 102, 612, 713, 1209, 1334, 1649
FauI CCCGC 2 cut(s) 1120, 1629
FauNDI CATATG 1 cut(s) 1441
FokI GGATG 2 cut(s) 271, 921
FriOI GRGCYC 4 cut(s) 460, 885, 1147, 1194
FspBI CTAG 8 cut(s) 65, 150, 603, 638, 845, 1020, 1104, 1548
GsaI CCCAGC 1 cut(s) 1140
HaeIII GGCC 6 cut(s) 135, 356, 687, 725, 798, 1620
HapII CCGG 1 cut(s) 357
HgaI GACGC 1 cut(s) 1479
Hin1I GRCGYC 1 cut(s) 1490
Hin1II CATG 6 cut(s) 106, 616, 717, 1213, 1338, 1653
HincII GTYRAC 2 cut(s) 173, 1101
HindII GTYRAC 2 cut(s) 173, 1101
HindIII AAGCTT 2 cut(s) 447, 1367
HinfI GANTC 4 cut(s) 20, 55, 666, 1341
HpaII CCGG 1 cut(s) 357
HphI GGTGA 2 cut(s) 641, 847
Hpy166II GTNNAC 8 cut(s) 173, 610, 1101, 1245, 1307, 1513, 1515, 1525
Hpy188I TCNGA 6 cut(s) 54, 60, 118, 212, 410, 1642
Hpy188III TCNNGA 7 cut(s) 24, 44, 490, 578, 1210, 1265, 1345
Hpy8I GTNNAC 8 cut(s) 173, 610, 1101, 1245, 1307, 1513, 1515, 1525
HpyAV CCTTC 5 cut(s) 17, 122, 496, 518, 1641
HpyCH4III ACNGT 3 cut(s) 249, 472, 918
HpyCH4IV ACGT 1 cut(s) 899
HpyCH4V TGCA 7 cut(s) 713, 733, 781, 1028, 1334, 1358, 1430
HpyF10VI GCNNNNNNNGC 9 cut(s) 132, 141, 150, 184, 353, 455, 1025, 1151, 1427
HpyF3I CTNAG 7 cut(s) 328, 407, 476, 923, 1365, 1557, 1641
HpySE526I ACGT 1 cut(s) 899
Hsp92I GRCGYC 1 cut(s) 1490
Hsp92II CATG 6 cut(s) 106, 616, 717, 1213, 1338, 1653
LguI GCTCTTC 3 cut(s) 329, 1199, 1546
LmnI GCTCC 4 cut(s) 195, 455, 1373, 1561
Lsp1109I GCAGC 9 cut(s) 153, 156, 196, 199, 204, 697, 1040, 1141, 1318
LweI GCATC 2 cut(s) 405, 720
MaeI CTAG 8 cut(s) 65, 150, 603, 638, 845, 1020, 1104, 1548
MaeII ACGT 1 cut(s) 899
MaeIII GTNAC 4 cut(s) 41, 243, 1007, 1473
MboII GAAGA 9 cut(s) 104, 107, 251, 316, 661, 1160, 1186, 1563, 1622
MhlI GDGCHC 4 cut(s) 460, 885, 1147, 1194
MlsI TGGCCA 1 cut(s) 135
MluNI TGGCCA 1 cut(s) 135
MlyI GAGTC 2 cut(s) 675, 1350
MmeI TCCRAC 1 cut(s) 1090
Mox20I TGGCCA 1 cut(s) 135
MscI TGGCCA 1 cut(s) 135
MseI TTAA 3 cut(s) 674, 1085, 1383
MslI CAYNNNNRTG 2 cut(s) 107, 538
Msp20I TGGCCA 1 cut(s) 135
MspA1I CMGCKG 1 cut(s) 1331
MspCI CTTAAG 1 cut(s) 673
MspI CCGG 1 cut(s) 357
MspR9I CCNGG 1 cut(s) 12
MvaI CCWGG 1 cut(s) 12
MvnI CGCG 2 cut(s) 539, 1178
MwoI GCNNNNNNNGC 9 cut(s) 132, 141, 150, 184, 353, 455, 1025, 1151, 1427
NdeI CATATG 1 cut(s) 1441
NheI GCTAGC 1 cut(s) 637
NlaIII CATG 6 cut(s) 106, 616, 717, 1213, 1338, 1653
NlaIV GGNNCC 1 cut(s) 432
NmeAIII GCCGAG 2 cut(s) 701, 1166
NmuCI GTSAC 1 cut(s) 41
NspI RCATGY 2 cut(s) 717, 1338
PaeR7I CTCGAG 1 cut(s) 24
PagI TCATGA 1 cut(s) 1209
PceI AGGCCT 1 cut(s) 687
PciSI GCTCTTC 3 cut(s) 329, 1199, 1546
PfeI GAWTC 2 cut(s) 20, 55
PleI GAGTC 2 cut(s) 674, 1349
PpsI GAGTC 2 cut(s) 674, 1349
PsiI TTATAA 1 cut(s) 35
Psp124BI GAGCTC 1 cut(s) 460
Psp6I CCWGG 1 cut(s) 10
PspFI CCCAGC 1 cut(s) 1136
PspGI CCWGG 1 cut(s) 10
PspN4I GGNNCC 1 cut(s) 432
PspPI GGNCC 5 cut(s) 269, 430, 724, 796, 1619
PstNI CAGNNNCTG 1 cut(s) 1355
PvuII CAGCTG 1 cut(s) 1331
RsaI GTAC 5 cut(s) 16, 101, 421, 1354, 1514
RsaNI GTAC 5 cut(s) 15, 100, 420, 1353, 1513
RseI CAYNNNNRTG 2 cut(s) 107, 538
SacI GAGCTC 1 cut(s) 460
SapI GCTCTTC 3 cut(s) 329, 1199, 1546
SaqAI TTAA 3 cut(s) 674, 1085, 1383
Sau96I GGNCC 5 cut(s) 269, 430, 724, 796, 1619
SchI GAGTC 2 cut(s) 675, 1350
ScrFI CCNGG 1 cut(s) 12
SduI GDGCHC 4 cut(s) 460, 885, 1147, 1194
SfaNI GCATC 2 cut(s) 405, 720
SfcI CTRYAG 1 cut(s) 179
Sfr274I CTCGAG 1 cut(s) 24
SinI GGWCC 2 cut(s) 269, 430
SlaI CTCGAG 1 cut(s) 24
SmiMI CAYNNNNRTG 2 cut(s) 107, 538
SmlI CTYRAG 3 cut(s) 24, 642, 673
SmoI CTYRAG 3 cut(s) 24, 642, 673
SseBI AGGCCT 1 cut(s) 687
SsiI CCGC 4 cut(s) 693, 1092, 1127, 1622
SspI AATATT 3 cut(s) 388, 550, 1591
SspMI CTAG 8 cut(s) 65, 150, 603, 638, 845, 1020, 1104, 1548
SstI GAGCTC 1 cut(s) 460
StuI AGGCCT 1 cut(s) 687
StyD4I CCNGG 1 cut(s) 10
StyI CCWWGG 2 cut(s) 872, 1119
TaaI ACNGT 3 cut(s) 249, 472, 918
TaiI ACGT 1 cut(s) 902
TaqI TCGA 1 cut(s) 25
TaqII GACCGA 1 cut(s) 1154
TatI WGTACW 3 cut(s) 99, 419, 1512
TauI GCSGC 1 cut(s) 1094
TfiI GAWTC 2 cut(s) 20, 55
Tru1I TTAA 3 cut(s) 674, 1085, 1383
Tru9I TTAA 3 cut(s) 674, 1085, 1383
TscAI CASTG 2 cut(s) 957, 1524
TseFI GTSAC 1 cut(s) 41
TseI GCWGC 9 cut(s) 141, 144, 184, 187, 217, 710, 1028, 1154, 1331
Tsp45I GTSAC 1 cut(s) 41
TspDTI ATGAA 8 cut(s) 105, 145, 990, 1198, 1266, 1383, 1458, 1623
TspGWI ACGGA 1 cut(s) 158
TspRI CASTG 2 cut(s) 957, 1524
Vha464I CTTAAG 1 cut(s) 673
VpaK11BI GGWCC 2 cut(s) 269, 430
XapI RAATTY 3 cut(s) 769, 817, 1260
XceI RCATGY 2 cut(s) 717, 1338
XcmI CCANNNNNNNNNTGG 1 cut(s) 564
XhoI CTCGAG 1 cut(s) 24
XspI CTAG 8 cut(s) 65, 150, 603, 638, 845, 1020, 1104, 1548
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.