Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000003

Orthogroup ID

OG0016038

Physical Location & Seq

Genomic Coordinates Reverse (-)

16152268 .. 16153775

Gene Structure 1508 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug03G0186300.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1347 bp

ATGGATAACAATCCAGTTCAAGAAGAACACAACCATGTCATCCCCACCTGGGAAATGAGCAGTGATCGCTTAGCTTCCATGTACCAGAAGATAGCAGACCCTCCACGACTCCTCAACAACGCCGCAGGCAAAGCCTCCTGCTGCATCTTCCGAGTCCCGCAGAGCCTCCTCGAGATAAACGGCAAGGCTTACCAGCCCCACATCGTCTCCATCGGCCCCTACCACCGCGGCGAGCCCCGCCTCAGAATGATCGAGGAGCACAAGTGGCGCTACCTGGGCTCGCTGCTTTCAAGAACAGAGCCCAAAGGGTTGACTCTACAGCACTACTTGAAGTCCATAGAGCCATTGGAAGCTAGAGCCAGGGAGTGTTACTCTGAGACCATCCATCTCAGCACTGATGAGTTCCTAGAAATGATGGTTGTTGATGGTTGCTTCTTAATTGAGTTGTTTCGAAAAGTTGGGCGTGTGGTTCGGTTTGAGCGTGATGATCCACTCTTTAATATGTTTTGGATACTACCGTTTATGGTCAGGGATTTTCTTCGGCTTGAGAACCAAATGCCCTACTTTATTCTTGAGCACTTGCATGATCTGATAAACAAGGCCGAAGATCGTAAAGACTCTGTGAAATCCTTGTCCTTGCTTGCTTTGCAATTCTTCAACATCCATATGATGAGGCCTGACCCTGTCATAGAAAAGCTCCATAGTCTTACAGGTATGCATTTGCTTGACTTATTGCGGTCAAGTGTGATACCAGCGGGTTATGCAGAGCCACAAAGAAGAAACAGTACACCAACGCACATGATACACCCTGTGTCTAAGCTTCGTCGCGCTGGTATCAAGCTTAATGTTAAGGAATTCGAAAGCTTCTTGGTGGTCAAATTCAAGCGTGGAGCAATTGAAATGCCTACCATCACAATCGACGATTTCATGTGCGCCTTCTTTTTGAACTGCGTGGCCTATGAGCAGTGCCACAAGTCATGCTCGAAACACATCACCACTTATGCCACATTGCTCGATTGCCTCGTGAACAGTTACAAGGATGTCGAGTATTTGTGTGACAGAAACATAATGGAGAATTGCTATGGGAACGATGGGGAAATTGCTTGTTTCATTAACAATATGGGGAAGGATGTGGCTTTTGATTATGATAGGTGTTATTTGTCGAAATTGTTCAATGATGTTCATCAGTATTACAGCAATAGTTGGCATGTTCAATGGGCTAGCTTCAAGTATAGATACTTTGACACACCGTGGTCGTTTATTTCAGCCTTGGCTGCTTTGATTTTGCTAATACTCACAGTGCTGCAGACCTTATACTCTGCTTTAAGTTATTATCATCATCATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000959 GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016071 GO:0016556 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0071704 GO:0080156 GO:0090304 GO:0090305 GO:0097159 GO:1900864 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DYW_deaminase PPR PPR_2

Protein Analysis

448

Amino Acids

52.41

Weight (kDa)

6.72

Isoelectric Point (pI)

49.43

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DUF247	PF03140	49 - 432	1.2e-119	Plant protein of unknown function

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0016038)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G13770 AT3G13770
fragaria_vesca	FvH4_6g22980 FvH4_6g22980
malus_domestica	MD14G1064300.v1.1
prunus_persica	Prupe.7G069900_v2.0.a1
pyrus_communis	pycom14g05200
rosa_chinensis	RchiOBHm_Chr3g0480771
rosa_laevigata	RLG00000023453
rosa_multiflora	Rmu_sc0003902.1_g000010 Rmu_sc0007944.1_g000002
rosa_roxburghii	Rroxscaffold_6G00401080
rosa_rugosa	Rorug03G0186300
rosa_samantha	Rh3DG263400
rosa_wichuraiana	Rw3G021300

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccII	CGCG	2 cut(s)	228, 828
AciI	CCGC	7 cut(s)	123, 158, 226, 228, 238, 736, 755
AclWI	GGATC	1 cut(s)	482
AcsI	RAATTY	2 cut(s)	854, 878
AdeI	CACNNNGTG	2 cut(s)	811, 1251
AfaI	GTAC	2 cut(s)	83, 787
AfiI	CCNNNNNNNGG	1 cut(s)	49
AjnI	CCWGG	3 cut(s)	47, 273, 359
AluBI	AGCT	7 cut(s)	74, 353, 697, 820, 841, 864, 1224
AluI	AGCT	7 cut(s)	74, 353, 697, 820, 841, 864, 1224
Alw21I	GWGCWC	2 cut(s)	261, 579
Alw26I	GTCTC	2 cut(s)	211, 371
AlwI	GGATC	1 cut(s)	482
Ama87I	CYCGRG	1 cut(s)	170
AoxI	GGCC	4 cut(s)	214, 600, 674, 954
ApeKI	GCWGC	4 cut(s)	141, 283, 1274, 1303
ApoI	RAATTY	2 cut(s)	854, 878
Asp700I	GAANNNNTTC	1 cut(s)	1169
AspLEI	GCGC	3 cut(s)	270, 830, 935
AspS9I	GGNCC	1 cut(s)	215
AsuHPI	GGTGA	1 cut(s)	985
AsuII	TTCGAA	2 cut(s)	451, 858
AsuNHI	GCTAGC	1 cut(s)	1220
AvaI	CYCGRG	1 cut(s)	170
BaeI	ACNNNNGTAYC	2 cut(s)	503, 536
BanII	GRGCYC	3 cut(s)	237, 281, 303
BarI	GAAGNNNNNNTAC	2 cut(s)	769, 801
BauI	CACGAG	1 cut(s)	1022
Bbv12I	GWGCWC	2 cut(s)	261, 579
BbvI	GCAGC	4 cut(s)	128, 270, 1261, 1290
BccI	CCATC	7 cut(s)	218, 389, 393, 409, 419, 917, 1085
BceAI	ACGGC	1 cut(s)	196
BciT130I	CCWGG	3 cut(s)	49, 275, 361
BciVI	GTATCC	1 cut(s)	504
BcoDI	GTCTC	2 cut(s)	211, 371
BfaI	CTAG	3 cut(s)	354, 407, 1221
BfmI	CTRYAG	2 cut(s)	317, 1304
BfoI	RGCGCY	1 cut(s)	271
BfuI	GTATCC	1 cut(s)	504
BisI	GCNGC	6 cut(s)	123, 142, 229, 284, 1275, 1304
BlpI	GCTNAGC	1 cut(s)	70
BlsI	GCNGC	6 cut(s)	124, 143, 230, 285, 1276, 1305
Bme1390I	CCNGG	3 cut(s)	49, 275, 361
BmeT110I	CYCGRG	1 cut(s)	170
BmgT120I	GGNCC	1 cut(s)	215
BmiI	GGNNCC	1 cut(s)	217
BmrFI	CCNGG	3 cut(s)	49, 275, 361
BmsI	GCATC	1 cut(s)	153
BmtI	GCTAGC	1 cut(s)	1224
BplI	GAGNNNNNCTC	2 cut(s)	356, 388
Bpu1102I	GCTNAGC	1 cut(s)	70
Bpu14I	TTCGAA	2 cut(s)	451, 858
BpuEI	CTTGAG	2 cut(s)	566, 593
BsaBI	GATNNNNATC	2 cut(s)	9, 1182
BsaI	GGTCTC	1 cut(s)	371
BsaJI	CCNNGG	6 cut(s)	48, 226, 274, 360, 1250, 1269
BsaXI	ACNNNNNCTCC	4 cut(s)	191, 221, 248, 278
Bsc4I	CCNNNNNNNGG	1 cut(s)	49
Bse1I	ACTGG	1 cut(s)	14
Bse3DI	GCAATG	1 cut(s)	1007
Bse8I	GATNNNNATC	2 cut(s)	9, 1182
BseBI	CCWGG	3 cut(s)	49, 275, 361
BseDI	CCNNGG	6 cut(s)	48, 226, 274, 360, 1250, 1269
BseGI	GGATG	5 cut(s)	39, 381, 660, 1045, 1135
BseJI	GATNNNNATC	2 cut(s)	9, 1182
BseLI	CCNNNNNNNGG	1 cut(s)	49
BseMI	GCAATG	1 cut(s)	1007
BseMII	CTCAG	3 cut(s)	256, 366, 403
BseNI	ACTGG	1 cut(s)	14
BseRI	GAGGAG	3 cut(s)	101, 158, 269
BseXI	GCAGC	4 cut(s)	128, 270, 1261, 1290
Bsh1236I	CGCG	2 cut(s)	228, 828
BshFI	GGCC	4 cut(s)	216, 602, 676, 956
BsiHKAI	GWGCWC	2 cut(s)	261, 579
BsiHKCI	CYCGRG	1 cut(s)	170
BslFI	GGGAC	1 cut(s)	140
BslI	CCNNNNNNNGG	1 cut(s)	49
BsmAI	GTCTC	2 cut(s)	211, 371
BsmBI	CGTCTC	1 cut(s)	211
BsmFI	GGGAC	1 cut(s)	140
BsnI	GGCC	4 cut(s)	216, 602, 676, 956
Bso31I	GGTCTC	1 cut(s)	371
BsoBI	CYCGRG	1 cut(s)	170
Bsp119I	TTCGAA	2 cut(s)	451, 858
Bsp1286I	GDGCHC	5 cut(s)	237, 261, 281, 303, 579
Bsp143I	GATC	5 cut(s)	64, 249, 487, 586, 607
Bsp1720I	GCTNAGC	1 cut(s)	70
BspACI	CCGC	7 cut(s)	123, 158, 226, 228, 238, 736, 755
BspANI	GGCC	4 cut(s)	216, 602, 676, 956
BspCNI	CTCAG	3 cut(s)	255, 367, 402
BspFNI	CGCG	2 cut(s)	228, 828
BspLI	GGNNCC	1 cut(s)	217
BspMAI	CTGCAG	1 cut(s)	1308
BspOI	GCTAGC	1 cut(s)	1224
BspPI	GGATC	1 cut(s)	482
BspT104I	TTCGAA	2 cut(s)	451, 858
BspTNI	GGTCTC	1 cut(s)	371
BsrDI	GCAATG	1 cut(s)	1007
BsrI	ACTGG	1 cut(s)	14
BssECI	CCNNGG	6 cut(s)	48, 226, 274, 360, 1250, 1269
BssMI	GATC	5 cut(s)	64, 249, 487, 586, 607
BssSI	CACGAG	1 cut(s)	1022
BssT1I	CCWWGG	1 cut(s)	1269
Bst2BI	CACGAG	1 cut(s)	1022
Bst2UI	CCWGG	3 cut(s)	49, 275, 361
Bst4CI	ACNGT	5 cut(s)	519, 785, 1031, 1251, 1300
BstBI	TTCGAA	2 cut(s)	451, 858
BstC8I	GCNNGC	5 cut(s)	127, 233, 281, 642, 1222
BstDEI	CTNAG	5 cut(s)	70, 242, 375, 389, 816
BstDSI	CCRYGG	2 cut(s)	226, 1250
BstF5I	GGATG	5 cut(s)	39, 381, 660, 1045, 1135
BstFNI	CGCG	2 cut(s)	228, 828
BstH2I	RGCGCY	1 cut(s)	271
BstHHI	GCGC	3 cut(s)	270, 830, 935
BstKTI	GATC	5 cut(s)	67, 252, 490, 589, 610
BstMAI	GTCTC	2 cut(s)	211, 371
BstMBI	GATC	5 cut(s)	64, 249, 487, 586, 607
BstMWI	GCNNNNNNNGC	8 cut(s)	66, 131, 237, 265, 276, 646, 761, 1274
BstNI	CCWGG	3 cut(s)	49, 275, 361
BstNSI	RCATGY	1 cut(s)	1211
BstSCI	CCNGG	3 cut(s)	47, 273, 359
BstSFI	CTRYAG	2 cut(s)	317, 1304
BstUI	CGCG	2 cut(s)	228, 828
BstV1I	GCAGC	4 cut(s)	128, 270, 1261, 1290
BsuI	GTATCC	1 cut(s)	504
BsuRI	GGCC	4 cut(s)	216, 602, 676, 956
BtgI	CCRYGG	2 cut(s)	226, 1250
BtsCI	GGATG	5 cut(s)	39, 381, 660, 1045, 1135
BtsI	GCAGTG	2 cut(s)	67, 971
BtsIMutI	CAGTG	4 cut(s)	67, 393, 971, 1305
Cac8I	GCNNGC	5 cut(s)	127, 233, 281, 642, 1222
CfoI	GCGC	3 cut(s)	270, 830, 935
Cfr13I	GGNCC	1 cut(s)	215
Cfr42I	CCGCGG	1 cut(s)	229
Csp6I	GTAC	2 cut(s)	82, 786
CviAII	CATG	7 cut(s)	35, 79, 584, 799, 928, 978, 1208
CviQI	GTAC	2 cut(s)	82, 786
DdeI	CTNAG	5 cut(s)	70, 242, 375, 389, 816
DpnI	GATC	5 cut(s)	66, 251, 489, 588, 609
DpnII	GATC	5 cut(s)	64, 249, 487, 586, 607
DraIII	CACNNNGTG	2 cut(s)	811, 1251
Eco130I	CCWWGG	1 cut(s)	1269
Eco147I	AGGCCT	1 cut(s)	676
Eco24I	GRGCYC	3 cut(s)	237, 281, 303
Eco31I	GGTCTC	1 cut(s)	371
Eco88I	CYCGRG	1 cut(s)	170
EcoRI	GAATTC	1 cut(s)	854
EcoRII	CCWGG	3 cut(s)	47, 273, 359
EcoT14I	CCWWGG	1 cut(s)	1269
EcoT22I	ATGCAT	1 cut(s)	720
EcoT38I	GRGCYC	3 cut(s)	237, 281, 303
ErhI	CCWWGG	1 cut(s)	1269
Esp3I	CGTCTC	1 cut(s)	211
FaeI	CATG	7 cut(s)	38, 82, 587, 802, 931, 981, 1211
FaqI	GGGAC	1 cut(s)	140
FatI	CATG	7 cut(s)	34, 78, 583, 798, 927, 977, 1207
FauI	CCCGC	3 cut(s)	165, 245, 748
FauNDI	CATATG	1 cut(s)	666
Fnu4HI	GCNGC	6 cut(s)	123, 142, 229, 284, 1275, 1304
FokI	GGATG	5 cut(s)	26, 368, 647, 1052, 1142
FriOI	GRGCYC	3 cut(s)	237, 281, 303
Fsp4HI	GCNGC	6 cut(s)	123, 142, 229, 284, 1275, 1304
FspBI	CTAG	3 cut(s)	354, 407, 1221
GlaI	GCGC	3 cut(s)	269, 829, 934
GluI	GCNGC	6 cut(s)	123, 142, 229, 284, 1275, 1304
HaeII	RGCGCY	1 cut(s)	271
HaeIII	GGCC	4 cut(s)	216, 602, 676, 956
HhaI	GCGC	3 cut(s)	270, 830, 935
Hin1II	CATG	7 cut(s)	38, 82, 587, 802, 931, 981, 1211
Hin6I	GCGC	3 cut(s)	268, 828, 933
HinP1I	GCGC	3 cut(s)	268, 828, 933
HincII	GTYRAC	1 cut(s)	312
HindII	GTYRAC	1 cut(s)	312
HindIII	AAGCTT	3 cut(s)	818, 839, 862
HinfI	GANTC	4 cut(s)	108, 153, 313, 617
HphI	GGTGA	1 cut(s)	985
Hpy166II	GTNNAC	3 cut(s)	312, 788, 1027
Hpy188I	TCNGA	4 cut(s)	152, 245, 376, 591
Hpy188III	TCNNGA	5 cut(s)	20, 172, 291, 572, 1024
Hpy8I	GTNNAC	3 cut(s)	312, 788, 1027
Hpy99I	CGWCG	2 cut(s)	828, 923
HpyAV	CCTTC	2 cut(s)	946, 1120
HpyCH4III	ACNGT	5 cut(s)	519, 785, 1031, 1251, 1300
HpyCH4V	TGCA	6 cut(s)	144, 583, 649, 718, 764, 1306
HpyF10VI	GCNNNNNNNGC	8 cut(s)	66, 131, 237, 265, 276, 646, 761, 1274
HpyF3I	CTNAG	5 cut(s)	70, 242, 375, 389, 816
Hsp92II	CATG	7 cut(s)	38, 82, 587, 802, 931, 981, 1211
HspAI	GCGC	3 cut(s)	268, 828, 933
KspI	CCGCGG	1 cut(s)	229
Kzo9I	GATC	5 cut(s)	64, 249, 487, 586, 607
LmnI	GCTCC	3 cut(s)	256, 702, 890
Lsp1109I	GCAGC	4 cut(s)	128, 270, 1261, 1290
LweI	GCATC	1 cut(s)	153
MaeI	CTAG	3 cut(s)	354, 407, 1221
MaeIII	GTNAC	3 cut(s)	368, 1031, 1055
MalI	GATC	5 cut(s)	66, 251, 489, 588, 609
MboI	GATC	5 cut(s)	64, 249, 487, 586, 607
MboII	GAAGA	7 cut(s)	35, 100, 139, 530, 617, 646, 789
MfeI	CAATTG	1 cut(s)	894
MhlI	GDGCHC	5 cut(s)	237, 261, 281, 303, 579
MluCI	AATT	8 cut(s)	438, 650, 854, 878, 894, 1075, 1098, 1166
MlyI	GAGTC	4 cut(s)	102, 162, 307, 611
MnlI	CCTC	9 cut(s)	111, 122, 145, 176, 179, 247, 251, 666, 1031
Mph1103I	ATGCAT	1 cut(s)	720
MroXI	GAANNNNTTC	1 cut(s)	1169
MseI	TTAA	6 cut(s)	437, 498, 843, 849, 1113, 1325
MslI	CAYNNNNRTG	3 cut(s)	33, 582, 665
MspA1I	CMGCKG	2 cut(s)	228, 755
MspR9I	CCNGG	3 cut(s)	49, 275, 361
MunI	CAATTG	1 cut(s)	894
MvaI	CCWGG	3 cut(s)	49, 275, 361
MvnI	CGCG	2 cut(s)	228, 828
MwoI	GCNNNNNNNGC	8 cut(s)	66, 131, 237, 265, 276, 646, 761, 1274
NdeI	CATATG	1 cut(s)	666
NdeII	GATC	5 cut(s)	64, 249, 487, 586, 607
NheI	GCTAGC	1 cut(s)	1220
NlaIII	CATG	7 cut(s)	38, 82, 587, 802, 931, 981, 1211
NlaIV	GGNNCC	1 cut(s)	217
NmuCI	GTSAC	1 cut(s)	1055
NsiI	ATGCAT	1 cut(s)	720
NspI	RCATGY	1 cut(s)	1211
NspV	TTCGAA	2 cut(s)	451, 858
PaeR7I	CTCGAG	1 cut(s)	170
PceI	AGGCCT	1 cut(s)	676
PcsI	WCGNNNNNNNCGW	2 cut(s)	478, 1020
PdmI	GAANNNNTTC	1 cut(s)	1169
PflFI	GACNNNGTC	1 cut(s)	683
PkrI	GCNGC	6 cut(s)	124, 143, 230, 285, 1276, 1305
PleI	GAGTC	4 cut(s)	102, 161, 307, 611
PpsI	GAGTC	4 cut(s)	102, 161, 307, 611
Psp6I	CCWGG	3 cut(s)	47, 273, 359
PspGI	CCWGG	3 cut(s)	47, 273, 359
PspN4I	GGNNCC	1 cut(s)	217
PspPI	GGNCC	1 cut(s)	215
PstI	CTGCAG	1 cut(s)	1308
PsyI	GACNNNGTC	1 cut(s)	683
RsaI	GTAC	2 cut(s)	83, 787
RsaNI	GTAC	2 cut(s)	82, 786
RseI	CAYNNNNRTG	3 cut(s)	33, 582, 665
SacII	CCGCGG	1 cut(s)	229
SaqAI	TTAA	6 cut(s)	437, 498, 843, 849, 1113, 1325
SatI	GCNGC	6 cut(s)	123, 142, 229, 284, 1275, 1304
Sau3AI	GATC	5 cut(s)	64, 249, 487, 586, 607
Sau96I	GGNCC	1 cut(s)	215
SchI	GAGTC	4 cut(s)	102, 162, 307, 611
ScrFI	CCNGG	3 cut(s)	49, 275, 361
SduI	GDGCHC	5 cut(s)	237, 261, 281, 303, 579
SfaNI	GCATC	1 cut(s)	153
SfcI	CTRYAG	2 cut(s)	317, 1304
Sfr274I	CTCGAG	1 cut(s)	170
Sfr303I	CCGCGG	1 cut(s)	229
SfuI	TTCGAA	2 cut(s)	451, 858
SgrBI	CCGCGG	1 cut(s)	229
SlaI	CTCGAG	1 cut(s)	170
SmiMI	CAYNNNNRTG	3 cut(s)	33, 582, 665
SmlI	CTYRAG	3 cut(s)	170, 545, 572
SmoI	CTYRAG	3 cut(s)	170, 545, 572
Sse9I	AATT	8 cut(s)	438, 650, 854, 878, 894, 1075, 1098, 1166
SseBI	AGGCCT	1 cut(s)	676
SsiI	CCGC	7 cut(s)	123, 158, 226, 228, 238, 736, 755
SspMI	CTAG	3 cut(s)	354, 407, 1221
StuI	AGGCCT	1 cut(s)	676
StyD4I	CCNGG	3 cut(s)	47, 273, 359
StyI	CCWWGG	1 cut(s)	1269
TaaI	ACNGT	5 cut(s)	519, 785, 1031, 1251, 1300
TaqI	TCGA	9 cut(s)	171, 252, 451, 858, 918, 983, 1014, 1044, 1163
TasI	AATT	8 cut(s)	438, 650, 854, 878, 894, 1075, 1098, 1166
TatI	WGTACW	1 cut(s)	785
TauI	GCSGC	2 cut(s)	125, 231
Tru1I	TTAA	6 cut(s)	437, 498, 843, 849, 1113, 1325
Tru9I	TTAA	6 cut(s)	437, 498, 843, 849, 1113, 1325
TscAI	CASTG	4 cut(s)	67, 400, 971, 1305
TseFI	GTSAC	1 cut(s)	1055
TseI	GCWGC	4 cut(s)	141, 283, 1274, 1303
Tsp45I	GTSAC	1 cut(s)	1055
TspDTI	ATGAA	3 cut(s)	916, 1099, 1172
TspRI	CASTG	4 cut(s)	67, 400, 971, 1305
Tth111I	GACNNNGTC	1 cut(s)	683
XapI	RAATTY	2 cut(s)	854, 878
XceI	RCATGY	1 cut(s)	1211
XcmI	CCANNNNNNNNNTGG	1 cut(s)	343
XhoI	CTCGAG	1 cut(s)	170
XmnI	GAANNNNTTC	1 cut(s)	1169
XspI	CTAG	3 cut(s)	354, 407, 1221
Zsp2I	ATGCAT	1 cut(s)	720

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.