Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000003

Orthogroup ID

OG0019903

Physical Location & Seq

Genomic Coordinates Forward (+)

24417070 .. 24420842

Gene Structure 3773 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug03G0256400.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1590 bp

ATGGGTAATTGCTGTGTGACCCCTCCCCAAACGGGTTCGCCCATAAAGAACAAGAAGAACAGGCCGAACCCATTTGCCATAGACTACGCCGTCACCCATGGCGGCAGCAAGCTCTCCGTCTTGAAAGACCCAACCGGCACCGAAATCGAGCAGACATACGAACTCGGCCGCGAGCTCGGCCGCGGAGAGTTCGGAATCACCTATCTCTGTACCGACAAGGTCACCAGTGAGAAGTTCGCTTGCAAATCGATTTCCAAGCAGAAGCTGCGGACGGCTGTGGATATCGAGGATGTGAGGAGGGAGGTTGAGATCATGAAGCATTTGCCTAAGCACCCCAATATTGTGAGCTTGAAAGATACGTATGAGGATGATAATGCGGTCCACCTTGTTATGGAGCTCTGTGAGGGCGGTGAGCTCTTCGATCGGATTGTGTCCAGAGGGCATTACACGGAGCGTGCCGCCGCTGCTGTCACTAAGACTATTGTGGAAGTTGTTCAGATGTGCCACAAGCATGGTGTGATGCATCGGGATCTCAAACCTGAGAACTTTTTATTCGCAAACAAGAAGGAAACAGCGTCTTTGAAGGCAATTGATTTCGGGCTGTCGGTCTTTTTTAAGCCTGGTGAAAAATTTAGTGAAATTGTTGGAAGTCCGTACTACATGGCTCCCGAGGTGCTAAGGCGCAATTATGGTCCTGAAGTTGATGTGTGGAGTGCTGGAGTTATACTTTACATTTTACTTTGTGGTGTTCCACCTTTCTGGGCAGAAACTGAACAGGGAGTTGCACAAGCAATTATACGGTCTGTTGTAGATTTTAAGAGGGACCCCTGGCCTAAGGTTTCTGATAATGCAAAAGACCTTGTGAAGAAGATGCTTAATCCTGACCCGAAGCAGAGGCTTACAGCTCAGCAAGTTCTAGATCATACTTGGTTGCAAAATGCAAAGAAAGCTCCAAATGTTTCTTTAGGTGAAACAGTGAGAGCAAGGCTCAAGCAATTCTCTGTAATGAACAAGCTTAAGAAAAGTGCACTCAAGGTTATAGCTGAGCATTTGTCACAGGATGAAGTTGCTGGCATACAAGAGGGATTTAAGCTCATGGATACTAGCAACCACGGCAAGATTAACATTGATGAGCTAAGAATTGGGTTGCATAAACTTGGCCATCAGATACCTGATACTGATCTTCATATCCTAATGGAAGCTGGTGATGTAGATAATGATGGGTATCTGGACTATGGAGAGTTTGTAGCCATTTCTGTTCACCTAAGAAGGATGGGCAATGATGATGAGCACCTTCGCAAAGCATTCGACTTCTTTGATCAAAACAAAAGTGGGTACATTGAAGTTGAGGAGTTGCGAACTGCCTTGGCTCCTGAAGTTGATGATAACGTTGAAGATGTTATTAGTGCCATTATCAATGACGTGGATACAGACAAGGATGGAAAAATAAGTTACGAGGAGTTTGCCGCCATGATGAAGGCCGGCACAGATTGGAGAAAAGCGTCAAGGCAGTATTCACGAGATCGGTTCAATAGTCTCAGTTTGAAATTGATGAGGGATGGATCGTTGGAAGGTAAAACCGAGAGTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0001763 GO:0003006 GO:0003674 GO:0003700 GO:0006355 GO:0007275 GO:0008150 GO:0009653 GO:0009791 GO:0009888 GO:0009889 GO:0009908 GO:0009933 GO:0009987 GO:0010014 GO:0010016 GO:0010223 GO:0010346 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0019827 GO:0022412 GO:0022414 GO:0031323 GO:0031326 GO:0032501 GO:0032502 GO:0032504 GO:0048367 GO:0048437 GO:0048438 GO:0048443 GO:0048466 GO:0048507 GO:0048532 GO:0048608 GO:0048646 GO:0048653 GO:0048731 GO:0048827 GO:0048856 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0061458 GO:0065007 GO:0080090 GO:0080166 GO:0090506 GO:0090567 GO:0098727 GO:0099402 GO:0140110 GO:1903506 GO:1905393 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Homeobox

Protein Analysis

529

Amino Acids

59.54

Weight (kDa)

6.02

Isoelectric Point (pI)

35.14

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	53 - 311	7e-81	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	54 - 306	1.1e-27	Protein tyrosine and serine/threonine kinase
ABC1	PF03109	128 - 207	7.9e-06	ABC1 atypical kinase-like domain
Kinase-like	PF14531	142 - 299	8.8e-06	Kinase-like
EF-hand_7	PF13499	359 - 417	5.1e-08	EF-hand domain pair
EF-hand_7	PF13499	430 - 493	5.5e-17	EF-hand domain pair
EF-hand_1	PF00036	432 - 456	4.2e-06	EF hand domain
EF-hand_6	PF13405	432 - 456	1.4e-06	EF-hand domain
EF-hand_1	PF00036	469 - 494	2.4e-07	EF hand domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0019903)

Species	Orthologous Gene IDs
rosa_chinensis	RchiOBHm_Chr3g0472761
rosa_roxburghii	Rroxscaffold_1G00038490 Rroxscaffold_2G00112860 Rroxscaffold_6G00390550 Rroxscaffold_7G00199430
rosa_rugosa	Rorug03G0256400 Rorug03G0300800

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	89
AccB1I	GGYRCC	1 cut(s)	137
AccII	CGCG	2 cut(s)	171, 183
AclI	AACGTT	1 cut(s)	1389
AclWI	GGATC	2 cut(s)	537, 1570
AcoI	YGGCCR	3 cut(s)	166, 178, 1159
AcsI	RAATTY	1 cut(s)	629
AcuI	CTGAAG	2 cut(s)	717, 1395
AfaI	GTAC	3 cut(s)	211, 656, 1337
AfiI	CCNNNNNNNGG	2 cut(s)	32, 391
AflII	CTTAAG	1 cut(s)	1016
AgsI	TTSAA	7 cut(s)	124, 352, 583, 1343, 1394, 1531, 1546
AjiI	CACGTC	1 cut(s)	1423
AjnI	CCWGG	2 cut(s)	619, 827
Alw21I	GWGCWC	5 cut(s)	177, 399, 417, 1030, 1293
Alw26I	GTCTC	1 cut(s)	1541
Alw44I	GTGCAC	1 cut(s)	1026
AlwI	GGATC	2 cut(s)	537, 1570
AlwNI	CAGNNNCTG	2 cut(s)	265, 770
Ama87I	CYCGRG	1 cut(s)	668
AoxI	GGCC	6 cut(s)	62, 166, 178, 830, 1159, 1479
ApaLI	GTGCAC	1 cut(s)	1026
ApeKI	GCWGC	3 cut(s)	105, 265, 464
ApoI	RAATTY	1 cut(s)	629
Asp700I	GAANNNNTTC	1 cut(s)	492
AspLEI	GCGC	1 cut(s)	684
AspS9I	GGNCC	3 cut(s)	379, 692, 823
AsuHPI	GGTGA	8 cut(s)	85, 190, 214, 422, 635, 980, 1217, 1253
AvaI	CYCGRG	1 cut(s)	668
AvaII	GGWCC	3 cut(s)	379, 692, 823
AxyI	CCTNAGG	1 cut(s)	834
BaeGI	GKGCMC	1 cut(s)	1030
BalI	TGGCCA	1 cut(s)	1161
BanI	GGYRCC	1 cut(s)	137
BanII	GRGCYC	3 cut(s)	177, 399, 417
BauI	CACGAG	1 cut(s)	1518
Bbv12I	GWGCWC	5 cut(s)	177, 399, 417, 1030, 1293
BbvI	GCAGC	3 cut(s)	117, 252, 451
BccI	CCATC	5 cut(s)	1170, 1214, 1267, 1433, 1553
BceAI	ACGGC	3 cut(s)	74, 288, 1129
BcgI	CGANNNNNNTGC	6 cut(s)	127, 161, 1288, 1322, 1445, 1479
BciT130I	CCWGG	2 cut(s)	621, 829
BciVI	GTATCC	2 cut(s)	1093, 1420
BclI	TGATCA	1 cut(s)	1318
BcoDI	GTCTC	1 cut(s)	1541
BfaI	CTAG	2 cut(s)	917, 1104
BfrI	CTTAAG	1 cut(s)	1016
BfuI	GTATCC	2 cut(s)	1093, 1420
BisI	GCNGC	9 cut(s)	103, 106, 169, 181, 266, 459, 462, 465, 1467
BlpI	GCTNAGC	2 cut(s)	906, 1044
BlsI	GCNGC	9 cut(s)	104, 107, 170, 182, 267, 460, 463, 466, 1468
Bme1390I	CCNGG	2 cut(s)	621, 829
Bme18I	GGWCC	3 cut(s)	379, 692, 823
BmeT110I	CYCGRG	1 cut(s)	668
BmgBI	CACGTC	1 cut(s)	1423
BmgT120I	GGNCC	3 cut(s)	379, 692, 823
BmiI	GGNNCC	5 cut(s)	139, 666, 824, 825, 1371
BmrFI	CCNGG	2 cut(s)	621, 829
BmsI	GCATC	3 cut(s)	510, 532, 861
BplI	GAGNNNNNCTC	2 cut(s)	972, 1004
BpmI	CTGGAG	1 cut(s)	738
Bpu10I	CCTNAGC	2 cut(s)	327, 677
Bpu1102I	GCTNAGC	2 cut(s)	906, 1044
BpuEI	CTTGAG	2 cut(s)	974, 1016
Bsa29I	ATCGAT	1 cut(s)	248
BsaAI	YACGTR	1 cut(s)	360
BsaBI	GATNNNNATC	2 cut(s)	1179, 1224
BsaJI	CCNNGG	6 cut(s)	97, 181, 669, 827, 1111, 1365
BsaXI	ACNNNNNCTCC	2 cut(s)	1230, 1260
Bsc4I	CCNNNNNNNGG	2 cut(s)	32, 391
Bse118I	RCCGGY	2 cut(s)	134, 1481
Bse1I	ACTGG	1 cut(s)	225
Bse21I	CCTNAGG	1 cut(s)	834
Bse3DI	GCAATG	1 cut(s)	1285
Bse8I	GATNNNNATC	2 cut(s)	1179, 1224
BseBI	CCWGG	2 cut(s)	621, 829
BseCI	ATCGAT	1 cut(s)	248
BseDI	CCNNGG	6 cut(s)	97, 181, 669, 827, 1111, 1365
BseGI	GGATG	6 cut(s)	295, 373, 1066, 1278, 1444, 1564
BseJI	GATNNNNATC	2 cut(s)	1179, 1224
BseLI	CCNNNNNNNGG	2 cut(s)	32, 391
BseMI	GCAATG	1 cut(s)	1285
BseMII	CTCAG	4 cut(s)	531, 920, 1035, 1552
BseNI	ACTGG	1 cut(s)	225
BseRI	GAGGAG	3 cut(s)	310, 1364, 1472
BseSI	GKGCMC	1 cut(s)	1030
BseX3I	CGGCCG	2 cut(s)	166, 178
BseXI	GCAGC	3 cut(s)	117, 252, 451
Bsh1236I	CGCG	2 cut(s)	171, 183
Bsh1285I	CGRYCG	3 cut(s)	169, 181, 424
BshFI	GGCC	6 cut(s)	64, 168, 180, 832, 1161, 1481
BshNI	GGYRCC	1 cut(s)	137
BshVI	ATCGAT	1 cut(s)	248
BsiEI	CGRYCG	3 cut(s)	169, 181, 424
BsiHKAI	GWGCWC	5 cut(s)	177, 399, 417, 1030, 1293
BsiHKCI	CYCGRG	1 cut(s)	668
BsiSI	CCGG	2 cut(s)	135, 1482
BslFI	GGGAC	1 cut(s)	836
BslI	CCNNNNNNNGG	2 cut(s)	32, 391
BsmAI	GTCTC	1 cut(s)	1541
BsmFI	GGGAC	1 cut(s)	836
BsmI	GAATGC	1 cut(s)	1304
BsnI	GGCC	6 cut(s)	64, 168, 180, 832, 1161, 1481
BsoBI	CYCGRG	1 cut(s)	668
Bsp1286I	GDGCHC	5 cut(s)	177, 399, 417, 1030, 1293
Bsp143I	GATC	8 cut(s)	309, 421, 529, 919, 1180, 1318, 1522, 1562
Bsp1720I	GCTNAGC	2 cut(s)	906, 1044
Bsp19I	CCATGG	1 cut(s)	97
BspANI	GGCC	6 cut(s)	64, 168, 180, 832, 1161, 1481
BspCNI	CTCAG	4 cut(s)	532, 919, 1036, 1551
BspDI	ATCGAT	1 cut(s)	248
BspFNI	CGCG	2 cut(s)	171, 183
BspHI	TCATGA	1 cut(s)	312
BspLI	GGNNCC	5 cut(s)	139, 666, 824, 825, 1371
BspPI	GGATC	2 cut(s)	537, 1570
BspQI	GCTCTTC	1 cut(s)	422
BspT107I	GGYRCC	1 cut(s)	137
BspTI	CTTAAG	1 cut(s)	1016
BsrDI	GCAATG	1 cut(s)	1285
BsrFI	RCCGGY	2 cut(s)	134, 1481
BsrI	ACTGG	1 cut(s)	225
BssAI	RCCGGY	2 cut(s)	134, 1481
BssECI	CCNNGG	6 cut(s)	97, 181, 669, 827, 1111, 1365
BssMI	GATC	8 cut(s)	309, 421, 529, 919, 1180, 1318, 1522, 1562
BssSI	CACGAG	1 cut(s)	1518
BssT1I	CCWWGG	2 cut(s)	97, 1365
Bst2BI	CACGAG	1 cut(s)	1518
Bst2UI	CCWGG	2 cut(s)	621, 829
Bst4CI	ACNGT	2 cut(s)	801, 976
Bst6I	CTCTTC	1 cut(s)	422
BstAFI	CTTAAG	1 cut(s)	1016
BstAPI	GCANNNNNTGC	1 cut(s)	265
BstBAI	YACGTR	1 cut(s)	360
BstC8I	GCNNGC	6 cut(s)	110, 173, 241, 456, 1072, 1483
BstDSI	CCRYGG	3 cut(s)	97, 181, 1111
BstEII	GGTNACC	1 cut(s)	220
BstF5I	GGATG	6 cut(s)	295, 373, 1066, 1278, 1444, 1564
BstFNI	CGCG	2 cut(s)	171, 183
BstHHI	GCGC	1 cut(s)	684
BstKTI	GATC	8 cut(s)	312, 424, 532, 922, 1183, 1321, 1525, 1565
BstMAI	GTCTC	1 cut(s)	1541
BstMBI	GATC	8 cut(s)	309, 421, 529, 919, 1180, 1318, 1522, 1562
BstMCI	CGRYCG	3 cut(s)	169, 181, 424
BstMWI	GCNNNNNNNGC	5 cut(s)	177, 265, 464, 947, 1113
BstNI	CCWGG	2 cut(s)	621, 829
BstPI	GGTNACC	1 cut(s)	220
BstSCI	CCNGG	2 cut(s)	619, 827
BstSLI	GKGCMC	1 cut(s)	1030
BstSNI	TACGTA	1 cut(s)	360
BstUI	CGCG	2 cut(s)	171, 183
BstV1I	GCAGC	3 cut(s)	117, 252, 451
BstX2I	RGATCY	1 cut(s)	529
BstXI	CCANNNNNNTGG	2 cut(s)	512, 759
BstYI	RGATCY	1 cut(s)	529
BstZI	CGGCCG	2 cut(s)	166, 178
Bsu15I	ATCGAT	1 cut(s)	248
Bsu36I	CCTNAGG	1 cut(s)	834
BsuI	GTATCC	2 cut(s)	1093, 1420
BsuRI	GGCC	6 cut(s)	64, 168, 180, 832, 1161, 1481
BsuTUI	ATCGAT	1 cut(s)	248
BtgI	CCRYGG	3 cut(s)	97, 181, 1111
BtrI	CACGTC	1 cut(s)	1423
BtsCI	GGATG	6 cut(s)	295, 373, 1066, 1278, 1444, 1564
BtsIMutI	CAGTG	2 cut(s)	232, 981
Cac8I	GCNNGC	6 cut(s)	110, 173, 241, 456, 1072, 1483
CaiI	CAGNNNCTG	2 cut(s)	265, 770
CciI	TCATGA	1 cut(s)	312
CfoI	GCGC	1 cut(s)	684
Cfr10I	RCCGGY	2 cut(s)	134, 1481
Cfr13I	GGNCC	3 cut(s)	379, 692, 823
Cfr42I	CCGCGG	1 cut(s)	184
ClaI	ATCGAT	1 cut(s)	248
CseI	GACGC	2 cut(s)	564, 1491
Csp6I	GTAC	3 cut(s)	210, 655, 1336
CviAII	CATG	6 cut(s)	98, 313, 512, 661, 1096, 1471
CviQI	GTAC	3 cut(s)	210, 655, 1336
DpnI	GATC	8 cut(s)	311, 423, 531, 921, 1182, 1320, 1524, 1564
DpnII	GATC	8 cut(s)	309, 421, 529, 919, 1180, 1318, 1522, 1562
DrdI	GACNNNNNNGTC	1 cut(s)	89
DseDI	GACNNNNNNGTC	1 cut(s)	89
EaeI	YGGCCR	3 cut(s)	166, 178, 1159
EagI	CGGCCG	2 cut(s)	166, 178
Eam1104I	CTCTTC	1 cut(s)	422
EarI	CTCTTC	1 cut(s)	422
Ecl136II	GAGCTC	3 cut(s)	175, 397, 415
EclXI	CGGCCG	2 cut(s)	166, 178
Eco105I	TACGTA	1 cut(s)	360
Eco130I	CCWWGG	2 cut(s)	97, 1365
Eco24I	GRGCYC	3 cut(s)	177, 399, 417
Eco32I	GATATC	1 cut(s)	283
Eco47I	GGWCC	3 cut(s)	379, 692, 823
Eco52I	CGGCCG	2 cut(s)	166, 178
Eco53kI	GAGCTC	3 cut(s)	175, 397, 415
Eco57I	CTGAAG	2 cut(s)	717, 1395
Eco81I	CCTNAGG	1 cut(s)	834
Eco88I	CYCGRG	1 cut(s)	668
Eco91I	GGTNACC	1 cut(s)	220
EcoICRI	GAGCTC	3 cut(s)	175, 397, 415
EcoO109I	RGGNCCY	1 cut(s)	823
EcoO65I	GGTNACC	1 cut(s)	220
EcoRII	CCWGG	2 cut(s)	619, 827
EcoRV	GATATC	1 cut(s)	283
EcoT14I	CCWWGG	2 cut(s)	97, 1365
EcoT22I	ATGCAT	1 cut(s)	525
EcoT38I	GRGCYC	3 cut(s)	177, 399, 417
ErhI	CCWWGG	2 cut(s)	97, 1365
FaeI	CATG	6 cut(s)	101, 316, 515, 664, 1099, 1474
FaqI	GGGAC	1 cut(s)	836
FatI	CATG	6 cut(s)	97, 312, 511, 660, 1095, 1470
FbaI	TGATCA	1 cut(s)	1318
Fnu4HI	GCNGC	9 cut(s)	103, 106, 169, 181, 266, 459, 462, 465, 1467
FokI	GGATG	6 cut(s)	302, 380, 1073, 1285, 1451, 1571
FriOI	GRGCYC	3 cut(s)	177, 399, 417
Fsp4HI	GCNGC	9 cut(s)	103, 106, 169, 181, 266, 459, 462, 465, 1467
FspBI	CTAG	2 cut(s)	917, 1104
GlaI	GCGC	1 cut(s)	683
GluI	GCNGC	9 cut(s)	103, 106, 169, 181, 266, 459, 462, 465, 1467
GsuI	CTGGAG	1 cut(s)	738
HaeIII	GGCC	6 cut(s)	64, 168, 180, 832, 1161, 1481
HapII	CCGG	2 cut(s)	135, 1482
HgaI	GACGC	2 cut(s)	564, 1491
HhaI	GCGC	1 cut(s)	684
Hin1II	CATG	6 cut(s)	101, 316, 515, 664, 1099, 1474
Hin6I	GCGC	1 cut(s)	682
HinP1I	GCGC	1 cut(s)	682
HindIII	AAGCTT	1 cut(s)	1013
HinfI	GANTC	1 cut(s)	195
HpaII	CCGG	2 cut(s)	135, 1482
HphI	GGTGA	8 cut(s)	85, 190, 214, 422, 635, 980, 1217, 1253
Hpy166II	GTNNAC	3 cut(s)	382, 1028, 1261
Hpy188I	TCNGA	5 cut(s)	194, 426, 498, 844, 1167
Hpy8I	GTNNAC	3 cut(s)	382, 1028, 1261
HpyAV	CCTTC	6 cut(s)	559, 577, 1263, 1304, 1471, 1565
HpyCH4III	ACNGT	2 cut(s)	801, 976
HpyCH4IV	ACGT	3 cut(s)	359, 1389, 1422
HpyCH4V	TGCA	8 cut(s)	243, 523, 785, 851, 934, 941, 1028, 1150
HpyF10VI	GCNNNNNNNGC	5 cut(s)	177, 265, 464, 947, 1113
HpySE526I	ACGT	3 cut(s)	359, 1389, 1422
Hsp92II	CATG	6 cut(s)	101, 316, 515, 664, 1099, 1474
HspAI	GCGC	1 cut(s)	682
KflI	GGGWCCC	1 cut(s)	823
KroI	GCCGGC	1 cut(s)	1481
KroNI	GCCGGC	1 cut(s)	1483
Ksp22I	TGATCA	1 cut(s)	1318
KspI	CCGCGG	1 cut(s)	184
Kzo9I	GATC	8 cut(s)	309, 421, 529, 919, 1180, 1318, 1522, 1562
LguI	GCTCTTC	1 cut(s)	422
LmnI	GCTCC	5 cut(s)	394, 451, 670, 955, 1375
Lsp1109I	GCAGC	3 cut(s)	117, 252, 451
LweI	GCATC	3 cut(s)	510, 532, 861
MaeI	CTAG	2 cut(s)	917, 1104
MaeII	ACGT	3 cut(s)	359, 1389, 1422
MaeIII	GTNAC	6 cut(s)	16, 91, 220, 469, 1053, 1451
MalI	GATC	8 cut(s)	311, 423, 531, 921, 1182, 1320, 1524, 1564
MboI	GATC	8 cut(s)	309, 421, 529, 919, 1180, 1318, 1522, 1562
MboII	GAAGA	6 cut(s)	67, 409, 877, 880, 1175, 1406
MfeI	CAATTG	1 cut(s)	588
MflI	RGATCY	1 cut(s)	529
MhlI	GDGCHC	5 cut(s)	177, 399, 417, 1030, 1293
MlsI	TGGCCA	1 cut(s)	1161
MluCI	AATT	9 cut(s)	7, 588, 629, 639, 685, 792, 995, 1140, 1547
MluNI	TGGCCA	1 cut(s)	1161
MmeI	TCCRAC	2 cut(s)	625, 1548
Mox20I	TGGCCA	1 cut(s)	1161
Mph1103I	ATGCAT	1 cut(s)	525
MroNI	GCCGGC	1 cut(s)	1481
MroXI	GAANNNNTTC	1 cut(s)	492
MscI	TGGCCA	1 cut(s)	1161
MseI	TTAA	7 cut(s)	615, 816, 876, 1017, 1089, 1122, 1588
MslI	CAYNNNNRTG	1 cut(s)	510
Msp20I	TGGCCA	1 cut(s)	1161
MspA1I	CMGCKG	2 cut(s)	183, 464
MspCI	CTTAAG	1 cut(s)	1016
MspI	CCGG	2 cut(s)	135, 1482
MspR9I	CCNGG	2 cut(s)	621, 829
MunI	CAATTG	1 cut(s)	588
Mva1269I	GAATGC	1 cut(s)	1304
MvaI	CCWGG	2 cut(s)	621, 829
MvnI	CGCG	2 cut(s)	171, 183
MwoI	GCNNNNNNNGC	5 cut(s)	177, 265, 464, 947, 1113
NaeI	GCCGGC	1 cut(s)	1483
NcoI	CCATGG	1 cut(s)	97
NdeII	GATC	8 cut(s)	309, 421, 529, 919, 1180, 1318, 1522, 1562
NgoMIV	GCCGGC	1 cut(s)	1481
NlaIII	CATG	6 cut(s)	101, 316, 515, 664, 1099, 1474
NlaIV	GGNNCC	5 cut(s)	139, 666, 824, 825, 1371
NmeAIII	GCCGAG	2 cut(s)	144, 156
NmuCI	GTSAC	5 cut(s)	16, 91, 220, 469, 1053
NsiI	ATGCAT	1 cut(s)	525
PagI	TCATGA	1 cut(s)	312
PciSI	GCTCTTC	1 cut(s)	422
PctI	GAATGC	1 cut(s)	1304
PdiI	GCCGGC	1 cut(s)	1483
PdmI	GAANNNNTTC	1 cut(s)	492
PfeI	GAWTC	1 cut(s)	195
PflFI	GACNNNGTC	1 cut(s)	218
PkrI	GCNGC	9 cut(s)	104, 107, 170, 182, 267, 460, 463, 466, 1468
Ple19I	CGATCG	1 cut(s)	424
Ppu21I	YACGTR	1 cut(s)	360
PpuMI	RGGWCCY	1 cut(s)	823
Psp124BI	GAGCTC	3 cut(s)	177, 399, 417
Psp1406I	AACGTT	1 cut(s)	1389
Psp5II	RGGWCCY	1 cut(s)	823
Psp6I	CCWGG	2 cut(s)	619, 827
PspEI	GGTNACC	1 cut(s)	220
PspGI	CCWGG	2 cut(s)	619, 827
PspN4I	GGNNCC	5 cut(s)	139, 666, 824, 825, 1371
PspPI	GGNCC	3 cut(s)	379, 692, 823
PspPPI	RGGWCCY	1 cut(s)	823
PstNI	CAGNNNCTG	2 cut(s)	265, 770
PsuI	RGATCY	1 cut(s)	529
PsyI	GACNNNGTC	1 cut(s)	218
PvuI	CGATCG	1 cut(s)	424
RsaI	GTAC	3 cut(s)	211, 656, 1337
RsaNI	GTAC	3 cut(s)	210, 655, 1336
RseI	CAYNNNNRTG	1 cut(s)	510
SacI	GAGCTC	3 cut(s)	177, 399, 417
SacII	CCGCGG	1 cut(s)	184
SapI	GCTCTTC	1 cut(s)	422
SaqAI	TTAA	7 cut(s)	615, 816, 876, 1017, 1089, 1122, 1588
SatI	GCNGC	9 cut(s)	103, 106, 169, 181, 266, 459, 462, 465, 1467
Sau3AI	GATC	8 cut(s)	309, 421, 529, 919, 1180, 1318, 1522, 1562
Sau96I	GGNCC	3 cut(s)	379, 692, 823
ScrFI	CCNGG	2 cut(s)	621, 829
SduI	GDGCHC	5 cut(s)	177, 399, 417, 1030, 1293
SfaNI	GCATC	3 cut(s)	510, 532, 861
Sfr303I	CCGCGG	1 cut(s)	184
SgrBI	CCGCGG	1 cut(s)	184
SinI	GGWCC	3 cut(s)	379, 692, 823
SmiMI	CAYNNNNRTG	1 cut(s)	510
SmlI	CTYRAG	3 cut(s)	989, 1016, 1031
SmoI	CTYRAG	3 cut(s)	989, 1016, 1031
SnaBI	TACGTA	1 cut(s)	360
Sse9I	AATT	9 cut(s)	7, 588, 629, 639, 685, 792, 995, 1140, 1547
SspI	AATATT	1 cut(s)	340
SspMI	CTAG	2 cut(s)	917, 1104
SstI	GAGCTC	3 cut(s)	177, 399, 417
StyD4I	CCNGG	2 cut(s)	619, 827
StyI	CCWWGG	2 cut(s)	97, 1365
TaaI	ACNGT	2 cut(s)	801, 976
TaiI	ACGT	3 cut(s)	362, 1392, 1425
TaqI	TCGA	5 cut(s)	147, 248, 285, 420, 1308
TaqII	GACCGA	1 cut(s)	595
TasI	AATT	9 cut(s)	7, 588, 629, 639, 685, 792, 995, 1140, 1547
TauI	GCSGC	6 cut(s)	105, 171, 183, 461, 464, 1469
TfiI	GAWTC	1 cut(s)	195
Tru1I	TTAA	7 cut(s)	615, 816, 876, 1017, 1089, 1122, 1588
Tru9I	TTAA	7 cut(s)	615, 816, 876, 1017, 1089, 1122, 1588
TscAI	CASTG	2 cut(s)	232, 981
TseFI	GTSAC	5 cut(s)	16, 91, 220, 469, 1053
TseI	GCWGC	3 cut(s)	105, 265, 464
Tsp45I	GTSAC	5 cut(s)	16, 91, 220, 469, 1053
TspDTI	ATGAA	5 cut(s)	329, 1022, 1077, 1175, 1490
TspGWI	ACGGA	3 cut(s)	106, 464, 642
TspRI	CASTG	2 cut(s)	232, 981
Tth111I	GACNNNGTC	1 cut(s)	218
Vha464I	CTTAAG	1 cut(s)	1016
VneI	GTGCAC	1 cut(s)	1026
VpaK11BI	GGWCC	3 cut(s)	379, 692, 823
XapI	RAATTY	1 cut(s)	629
XbaI	TCTAGA	1 cut(s)	916
XmnI	GAANNNNTTC	1 cut(s)	492
XspI	CTAG	2 cut(s)	917, 1104
Zsp2I	ATGCAT	1 cut(s)	525

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.