Rorug03G0300800

Homeodomain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0019903
Physical Location & Seq
Forward (+)
31625585 .. 31625968
384 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0300800.1

Sequence Viewer

Length: 384 bp
ATGCGTGTCCCCGAGGATGAACATCCCATTGCTATCGACAAGTTACTGGACGCGTTTTACGCCGCCGTATCCACCGAGCTGCCTATTGTGGTGGACATATGCGACATGATTTTGAGAATCGTCAGCGATCTCATATCTGAATCCATGGAAACGTATGAGCCCAATCTCGTCCCCGCGACAAGGTCATCGATTGAAGCCCTGGAGAGAGTGAACTTCACGTTGCCTTTTGAAGATCCATGTGTTATTTGTTTTGAGGATCTCACTATTGATCAAATGGTTATCCACTTGCCCTGTTCTCACTATTATCATGAGCATTGCATTTTCCAGTCACTGGAGAACAGTCACTTGTGTCCCCTCTGCCGCCACCCAATGCCAAACCTCTAG

Protein Analysis

127

Amino Acids

14.57

Weight (kDa)

4.49

Isoelectric Point (pI)

56.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-rbx1 PF12678 75 - 121 1e-07 RING-H2 zinc finger domain
zf-RING_2 PF13639 80 - 121 2.9e-12 Ring finger domain
zf-RING_11 PF17123 80 - 107 2.9e-08 RING-like zinc finger
zf-C3HC4_2 PF13923 80 - 120 5e-07 Zinc finger, C3HC4 type (RING finger)
zf-RING_5 PF14634 80 - 122 6.3e-06 zinc-RING finger domain
zf-RING_UBOX PF13445 80 - 118 1e-05 RING-type zinc-finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 331
AccII CGCG 2 cut(s) 53, 176
AciI CCGC 3 cut(s) 63, 174, 361
AclWI GGATC 2 cut(s) 227, 264
AfiI CCNNNNNNNGG 2 cut(s) 180, 331
AflIII ACRYGT 1 cut(s) 51
AgsI TTSAA 2 cut(s) 194, 230
AjnI CCWGG 1 cut(s) 198
AluBI AGCT 1 cut(s) 79
AluI AGCT 1 cut(s) 79
AlwI GGATC 2 cut(s) 227, 264
AlwNI CAGNNNCTG 1 cut(s) 331
Ama87I CYCGRG 1 cut(s) 11
ApeKI GCWGC 1 cut(s) 79
AvaI CYCGRG 1 cut(s) 11
BanII GRGCYC 1 cut(s) 162
BbvI GCAGC 1 cut(s) 66
BceAI ACGGC 1 cut(s) 50
BciT130I CCWGG 1 cut(s) 200
BciVI GTATCC 1 cut(s) 79
BclI TGATCA 1 cut(s) 268
BfaI CTAG 1 cut(s) 382
BfuI GTATCC 1 cut(s) 79
BisI GCNGC 3 cut(s) 63, 80, 361
BlsI GCNGC 3 cut(s) 64, 81, 362
Bme1390I CCNGG 1 cut(s) 200
BmeT110I CYCGRG 1 cut(s) 11
BmrFI CCNGG 1 cut(s) 200
BpmI CTGGAG 2 cut(s) 221, 353
Bsa29I ATCGAT 1 cut(s) 188
BsaBI GATNNNNATC 1 cut(s) 21
BsaJI CCNNGG 3 cut(s) 12, 144, 198
Bsc4I CCNNNNNNNGG 2 cut(s) 180, 331
Bse1I ACTGG 3 cut(s) 51, 325, 336
Bse3DI GCAATG 2 cut(s) 27, 313
Bse8I GATNNNNATC 1 cut(s) 21
BseBI CCWGG 1 cut(s) 200
BseCI ATCGAT 1 cut(s) 188
BseDI CCNNGG 3 cut(s) 12, 144, 198
BseGI GGATG 2 cut(s) 22, 22
BseJI GATNNNNATC 1 cut(s) 21
BseLI CCNNNNNNNGG 2 cut(s) 180, 331
BseMI GCAATG 2 cut(s) 27, 313
BseNI ACTGG 3 cut(s) 51, 325, 336
BseXI GCAGC 1 cut(s) 66
Bsh1236I CGCG 2 cut(s) 53, 176
BshVI ATCGAT 1 cut(s) 188
BsiHKCI CYCGRG 1 cut(s) 11
BslFI GGGAC 2 cut(s) 155, 336
BslI CCNNNNNNNGG 2 cut(s) 180, 331
BsmFI GGGAC 2 cut(s) 155, 336
BsoBI CYCGRG 1 cut(s) 11
Bsp1286I GDGCHC 1 cut(s) 162
Bsp143I GATC 4 cut(s) 127, 232, 256, 268
Bsp19I CCATGG 1 cut(s) 144
BspACI CCGC 3 cut(s) 63, 174, 361
BspDI ATCGAT 1 cut(s) 188
BspFNI CGCG 2 cut(s) 53, 176
BspHI TCATGA 1 cut(s) 307
BspPI GGATC 2 cut(s) 227, 264
BsrDI GCAATG 2 cut(s) 27, 313
BsrI ACTGG 3 cut(s) 51, 325, 336
BssECI CCNNGG 3 cut(s) 12, 144, 198
BssMI GATC 4 cut(s) 127, 232, 256, 268
BssT1I CCWWGG 1 cut(s) 144
Bst2UI CCWGG 1 cut(s) 200
Bst4CI ACNGT 1 cut(s) 341
BstDSI CCRYGG 1 cut(s) 144
BstF5I GGATG 2 cut(s) 22, 22
BstFNI CGCG 2 cut(s) 53, 176
BstKTI GATC 4 cut(s) 130, 235, 259, 271
BstMBI GATC 4 cut(s) 127, 232, 256, 268
BstMWI GCNNNNNNNGC 1 cut(s) 59
BstNI CCWGG 1 cut(s) 200
BstSCI CCNGG 1 cut(s) 198
BstUI CGCG 2 cut(s) 53, 176
BstV1I GCAGC 1 cut(s) 66
BstX2I RGATCY 2 cut(s) 232, 256
BstYI RGATCY 2 cut(s) 232, 256
Bsu15I ATCGAT 1 cut(s) 188
BsuI GTATCC 1 cut(s) 79
BsuTUI ATCGAT 1 cut(s) 188
BtgI CCRYGG 1 cut(s) 144
BtsCI GGATG 2 cut(s) 22, 22
BtsIMutI CAGTG 1 cut(s) 329
CaiI CAGNNNCTG 1 cut(s) 331
CciI TCATGA 1 cut(s) 307
ClaI ATCGAT 1 cut(s) 188
CseI GACGC 1 cut(s) 59
CviAII CATG 4 cut(s) 106, 145, 237, 308
CviJI RGCY 3 cut(s) 79, 160, 197
CviKI_1 RGCY 3 cut(s) 79, 160, 197
DpnI GATC 4 cut(s) 129, 234, 258, 270
DpnII GATC 4 cut(s) 127, 232, 256, 268
Eco130I CCWWGG 1 cut(s) 144
Eco24I GRGCYC 1 cut(s) 162
Eco88I CYCGRG 1 cut(s) 11
EcoRII CCWGG 1 cut(s) 198
EcoT14I CCWWGG 1 cut(s) 144
EcoT38I GRGCYC 1 cut(s) 162
ErhI CCWWGG 1 cut(s) 144
FaeI CATG 4 cut(s) 109, 148, 240, 311
FaiI YATR 8 cut(s) 98, 100, 107, 134, 146, 156, 238, 309
FaqI GGGAC 2 cut(s) 155, 336
FatI CATG 4 cut(s) 105, 144, 236, 307
FauI CCCGC 1 cut(s) 181
FauNDI CATATG 1 cut(s) 98
FbaI TGATCA 1 cut(s) 268
Fnu4HI GCNGC 3 cut(s) 63, 80, 361
FokI GGATG 2 cut(s) 9, 29
FriOI GRGCYC 1 cut(s) 162
Fsp4HI GCNGC 3 cut(s) 63, 80, 361
FspBI CTAG 1 cut(s) 382
GluI GCNGC 3 cut(s) 63, 80, 361
GsuI CTGGAG 2 cut(s) 221, 353
HgaI GACGC 1 cut(s) 59
Hin1II CATG 4 cut(s) 109, 148, 240, 311
HinfI GANTC 2 cut(s) 117, 140
Hpy166II GTNNAC 2 cut(s) 94, 211
Hpy188I TCNGA 1 cut(s) 139
Hpy188III TCNNGA 1 cut(s) 308
Hpy8I GTNNAC 2 cut(s) 94, 211
HpyCH4III ACNGT 1 cut(s) 341
HpyCH4IV ACGT 2 cut(s) 152, 218
HpyCH4V TGCA 1 cut(s) 318
HpyF10VI GCNNNNNNNGC 1 cut(s) 59
HpySE526I ACGT 2 cut(s) 152, 218
Hsp92II CATG 4 cut(s) 109, 148, 240, 311
Ksp22I TGATCA 1 cut(s) 268
Kzo9I GATC 4 cut(s) 127, 232, 256, 268
LpnPI CCDG 6 cut(s) 32, 185, 212, 304, 317, 338
Lsp1109I GCAGC 1 cut(s) 66
MaeI CTAG 1 cut(s) 382
MaeII ACGT 2 cut(s) 152, 218
MaeIII GTNAC 3 cut(s) 42, 327, 341
MalI GATC 4 cut(s) 129, 234, 258, 270
MboI GATC 4 cut(s) 127, 232, 256, 268
MboII GAAGA 1 cut(s) 242
MflI RGATCY 2 cut(s) 232, 256
MhlI GDGCHC 1 cut(s) 162
MluI ACGCGT 1 cut(s) 51
MnlI CCTC 3 cut(s) 7, 247, 365
MspR9I CCNGG 1 cut(s) 200
MvaI CCWGG 1 cut(s) 200
MvnI CGCG 2 cut(s) 53, 176
MwoI GCNNNNNNNGC 1 cut(s) 59
NcoI CCATGG 1 cut(s) 144
NdeI CATATG 1 cut(s) 98
NdeII GATC 4 cut(s) 127, 232, 256, 268
NlaIII CATG 4 cut(s) 109, 148, 240, 311
NmuCI GTSAC 2 cut(s) 327, 341
PagI TCATGA 1 cut(s) 307
PfeI GAWTC 2 cut(s) 117, 140
PflFI GACNNNGTC 1 cut(s) 181
PflMI CCANNNNNTGG 1 cut(s) 331
PkrI GCNGC 3 cut(s) 64, 81, 362
Psp6I CCWGG 1 cut(s) 198
PspGI CCWGG 1 cut(s) 198
PstNI CAGNNNCTG 1 cut(s) 331
PsuI RGATCY 2 cut(s) 232, 256
PsyI GACNNNGTC 1 cut(s) 181
SatI GCNGC 3 cut(s) 63, 80, 361
Sau3AI GATC 4 cut(s) 127, 232, 256, 268
ScrFI CCNGG 1 cut(s) 200
SduI GDGCHC 1 cut(s) 162
SetI ASST 5 cut(s) 81, 155, 185, 221, 381
SsiI CCGC 3 cut(s) 63, 174, 361
SspMI CTAG 1 cut(s) 382
StyD4I CCNGG 1 cut(s) 198
StyI CCWWGG 1 cut(s) 144
TaaI ACNGT 1 cut(s) 341
TaiI ACGT 2 cut(s) 155, 221
TaqI TCGA 2 cut(s) 36, 188
TauI GCSGC 2 cut(s) 65, 363
TfiI GAWTC 2 cut(s) 117, 140
TscAI CASTG 1 cut(s) 336
TseFI GTSAC 2 cut(s) 327, 341
TseI GCWGC 1 cut(s) 79
Tsp45I GTSAC 2 cut(s) 327, 341
TspDTI ATGAA 1 cut(s) 33
TspRI CASTG 1 cut(s) 336
Tth111I GACNNNGTC 1 cut(s) 181
Van91I CCANNNNNTGG 1 cut(s) 331
XspI CTAG 1 cut(s) 382
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.