Rorug03G0270900
ERF Family

Protein kinase superfamily protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0010054
Physical Location & Seq
Forward (+)
26713404 .. 26716908
3505 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0270900.1

Sequence Viewer

Length: 3390 bp
ATGGCCTCGAGCTCCCAAACAACCTCTTCATCAGCTCCTCAGTGGAAGCATGATGTGTTTCTCAGCTTCAGGGGTGAAGACACCCGGAAGGGTATTGTATCCTCTTTATACCATGAATTGAAAACCAGAGGAATACAAACATTCATGGATGACCAAGGGCTTCAGCAAGGGATGCCTATTTCTCCAAGTCTCCTAACAGCGATTGAGGAATCCAGGTGTGCAGTCGTTCTTCTATCGCCAAACTATGCTTCTTCTACATGGTGTTTGGATGAACTTGCAAAGATTCTCCAATGCTCTGAAGCCAACAAGACACTGCTTCTGCCAATTTTTTATAATGTGGATCCCTCTCATGTGCGACATCAAAAGGGAAGTTTTGCAGAAGCCTTCACTGAGCATGAAGAGAGGTATAGGCAAGGCAATGATGATGCAGAGAAGGTGAAGAAGTGGAGAGCTGCTTTAACACAAGTGGCCAATCTCGCTGGGTTGGATTCAAACAAATTTGGGTCTGACAGAGAACTTGTCCAACGCATTGTGGAACTTGTGTGCCAGAAAGTACGGCCTATTCCAATAATGCCGAGCAGCAATTTTATAGAATTTGAATCAACAAGAGAAGCCATGAATGAGCTGATGAACGCATTAGAAGATGATAAGGTCGCTGCCATTGGGGTCTATGGAATGGGAGGGGTTGGGAAGACAACCATGGTGAAACATGTCGGTGCACAAGCCCAAAAAATGGGGCTTTTTGATCGTGTGATTATGGCTGTTGTATCCCAAACCCCAGATCCAAGAAATATTCAAGGCACACTTGCGGATCTGCTGGGCTTGAAATTGGTGGAGGAGTCAGAAAGTGGACGAGCTGCTAGATTGCAGAGGGAGATATTTGGAAGAAATAAGATCCTTATAATCTTAGACGACATTTGGAATACACTTGACTTGTCAAGCATTGGAATTCCCAGCTACAACGAGCTTCAAATATGCAATTCCAAACTGCTATTCACCACAAGGAGATGGAATGTTAGCCATACCATGGAGAGCCAAGCAAAAATTCATCTCAATATCTTATCAGAAGATGATGCTTGGAACTTGTTTTTGAAGAAGGCAAGAAGGTCTTTTCAAAAGTCTAGCCCTTTCTATGAAGTAGCCAGAAAGGTAGCTAGAGAATGTGCTGGTCTCCCAGTTGCATTGATTGCAGTTGCGAGGGCACTTGGAGATAAAGAGCTTGAAGACTGGAAAGAAGCAGCTCGACAACTTGAGATGTCTCAACCTGCCAACCTTGACGAGGAGAAAGATGTGTTCAAGTGCATTAAGTTGAGCTATGATTACCTTGACAGTGACGACGCCAAGTCTTGCTTCTTGCTTTGCTGCCTATTCCCAGAAGATTATGACATCCGTGTTGAAGACTTGATGAGCTATGGGTTCGGAACAGGGTTGTTTCGACATGTCAACAGCACAATAGAATATGCCAGAGTCAGAGCACATTCGGTTACTAGATACCTTAAAGCTTCTTGTCTGCTTTTGGATGGGACAAAAGAAGGATATGTAAGGATGCATGATGTCATTCGAGATACGGCCATATCCATTGCATTCTCTGTAGATGGCCACACCTCTTTGGTGAAAACTAATTGTCATTTGAAAGATTGGTCGATGGATGCGGACGAAGGCTACTCAGCAATCTCACTAATCAAGAGCAACATTTGCAAGCTTCCCAACAAGTTGGTATGTTCAAAACTTCAGATTTTACTGCTACAAAAAAATGCTGATATAAGAGAGATCCCAATAACCTTTTTTCAAAGCCCCAATGATTTAAGGGTCTTAGATCTTAGCAACACTGGCATTTCATCACTGCCCCCATTATTCAGTCTCCTAACCAGACTCCAAGCTTTGCATTTAGATTATTGCAACTCCATGATTGACATATCCATACTTGGAAAACTGAAAAAGCTTGAGATTCTTAGTATGAGACGCTGTTACTCTATTAAGAAATTTCCAAAAGAGATTGGAAATTTGACGAATTTAAGAATGTTGGACTTGACTCATACCCTTTTTGAGACAGTTCCCTCTCAAGTGATATCAAGGTTGTATAGATTGGAAGAACTGTACATGAGATGTTCTTTTCGCGAGTGGGGTTGTAAAGCTGCGGGAGGCTGCTTGTGTTTTCTTTGTAAGAATGCTAGCTTTGATGAGTTAATTGGCTTGTCAAGTTTAAACACTTTGCAGGTTGCCGTATGTGGTGCAGAATGCTTGCCTCAAAACGTTGAGTTCAATCCAAATTGGGACAGGTTTTGTATATATGTACAAAGATACGACACGGTCGATTTGGGCTTTTTTGACGATTACTTTTTTGACGAGGTCTTCCCACATCGTTATTCAAGAACATTGATCATGACCGAAACCATGAATAACTTACCCGACTGGTTCATCAGTGTAGTGACAGAAAGAACAGATGATTTATGGTACAGAGGTCGCCATGGCTTAACTAACCTTCTCGAGGAACACGACCGTGGGAGATTACATGGACTCAAGTATTTGTGTATAGAATCTTCCGAATATGCCAAAGTGTTGTTGAACACAATAACATGGACTAGGGTGTCAAATGAACCTGTGTTTGAGAACTTGGAAGAGTTGGAAATCACCTGGTGTCTCTTGGAGGAGGTATGTGTCGGTGAATTACCACCTGGCTCTCTACGTAATCTTAAGAAATTGATGATGAGTATCTGTGATTGGTTGGTGAAACCGCTTTTACCGTCAAAGTTGTTGCACAGATTAGAGAGTCTGGAAGAACTATGTTGTTGTCGTATGCGGGCAATGAAATATGTGTTTGGATTTGAAGGGCATGAACCACAACACGTTGTCTTGACGAGGCTGAGAATGATTTCATTGAGATCCATGGACAGCTTAATAAACATATGGGATGGTCCTGCTCCATCTGGAATCTTCCAGAGTCTCAAAACCTTTTCCTTTGTTGATTGCCAGAAACTGGAATATCTCTTCACATCTGATGTAGCTCGATGTCTTTTGCAATTGGAAGACGTTTTGGTAGAGGATTGCGATAGCTTGGACAGAATAATTGAAGCGAGTGAGGAAATAGAGAGCAACAAGATCGTTCTTCCACAGTTGAAGAACTTGGTTTTAAAAGGTCTTCCAAAGCTCACAAGGCTGTCCAGTACAAGCAGCAGTACTATTGATATTGAAATCGAGTGCCCTACATTGGAACGCTTATGTGTCTCTGCGTGCCCCCAGTTATCAATCTCTGCTTCTGATTTCAACAGCAGCAACGAAGTCCAACTAGATGATTACCAGCTTTATTGGGACACACTACGGAATAGGATCGACGACGTGAGTTACCGCTCACACTTTCCACCTTCGATGAGGTATGTCTCCATGTCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

1129

Amino Acids

128.54

Weight (kDa)

5.61

Isoelectric Point (pI)

47.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 16 - 187 7.8e-53 TIR domain
TIR_2 PF13676 19 - 116 5e-17 TIR domain
NB-ARC PF00931 205 - 368 2.6e-29 NB-ARC domain
LRR_8 PF13855 576 - 634 2.6e-07 Leucine rich repeat
LRR_14 PF23598 587 - 701 2.2e-11 Leucine-rich repeat region
LRR_RPS2 PF23247 872 - 1017 1.5e-21 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 333, 902
AasI GACNNNNNNGTC 1 cut(s) 2587
Acc36I ACCTGC 2 cut(s) 1273, 2206
AccB7I CCANNNNNTGG 3 cut(s) 733, 1027, 2977
AccBSI CCGCTC 1 cut(s) 3348
AccII CGCG 1 cut(s) 2118
AciI CCGC 6 cut(s) 809, 1652, 2138, 2735, 2800, 3346
AclI AACGTT 1 cut(s) 2253
AclWI GGATC 8 cut(s) 335, 348, 776, 819, 889, 1765, 2877, 3335
AcoI YGGCCR 3 cut(s) 468, 1569, 1597
AcsI RAATTY 7 cut(s) 497, 593, 948, 1044, 1982, 2002, 2011
AcuI CTGAAG 4 cut(s) 52, 146, 318, 1715
AcyI GRCGYC 1 cut(s) 1338
AdeI CACNNNGTG 1 cut(s) 2637
AfaI GTAC 6 cut(s) 555, 2099, 2295, 2456, 3166, 3178
AfiI CCNNNNNNNGG 6 cut(s) 733, 1027, 1279, 2488, 2977, 3208
AflII CTTAAG 1 cut(s) 2693
AflIII ACRYGT 3 cut(s) 709, 1438, 2845
AhdI GACNNNNNGTC 1 cut(s) 1342
AjiI CACGTC 1 cut(s) 3337
AjnI CCWGG 3 cut(s) 212, 2633, 2674
AjuI GAANNNNNNNTTGG 8 cut(s) 232, 264, 977, 1009, 1029, 1061, 1334, 1366
AleI CACNNNNGTG 1 cut(s) 2499
Alw21I GWGCWC 3 cut(s) 14, 721, 1477
Alw44I GTGCAC 1 cut(s) 717
AlwI GGATC 8 cut(s) 335, 348, 776, 819, 889, 1765, 2877, 3335
AlwNI CAGNNNCTG 1 cut(s) 2977
Ama87I CYCGRG 2 cut(s) 7, 2486
AoxI GGCC 5 cut(s) 3, 468, 557, 1569, 1597
ApaLI GTGCAC 1 cut(s) 717
ApoI RAATTY 7 cut(s) 497, 593, 948, 1044, 1982, 2002, 2011
Asp700I GAANNNNTTC 1 cut(s) 2872
AspS9I GGNCC 1 cut(s) 2915
AsuC2I CCSGG 1 cut(s) 85
AsuHPI GGTGA 8 cut(s) 86, 448, 715, 988, 1624, 2623, 2675, 2740
AsuNHI GCTAGC 1 cut(s) 2171
AvaI CYCGRG 2 cut(s) 7, 2486
AvaII GGWCC 1 cut(s) 2915
BaeGI GKGCMC 4 cut(s) 721, 1204, 3203, 3236
BaeI ACNNNNGTAYC 2 cut(s) 2446, 2479
BalI TGGCCA 2 cut(s) 470, 1599
BamHI GGATCC 1 cut(s) 340
BanII GRGCYC 1 cut(s) 14
BarI GAAGNNNNNNTAC 2 cut(s) 2524, 2556
BbsI GAAGAC 7 cut(s) 84, 698, 1230, 1404, 2344, 3033, 3131
Bbv12I GWGCWC 3 cut(s) 14, 721, 1477
BccI CCATC 6 cut(s) 1002, 1514, 1589, 1639, 2906, 2932
BceAI ACGGC 3 cut(s) 572, 1584, 2207
BcgI CGANNNNNNTGC 2 cut(s) 3082, 3116
BciT130I CCWGG 3 cut(s) 214, 2635, 2676
BciVI GTATCC 2 cut(s) 109, 778
BclI TGATCA 1 cut(s) 2379
BcnI CCSGG 1 cut(s) 85
BfaI CTAG 7 cut(s) 861, 1122, 1155, 1488, 2172, 2583, 3287
BfmI CTRYAG 1 cut(s) 1590
BfrI CTTAAG 1 cut(s) 2693
BfuAI ACCTGC 2 cut(s) 1273, 2206
BfuI GTATCC 2 cut(s) 109, 778
BglII AGATCT 1 cut(s) 1816
BmcAI AGTACT 1 cut(s) 3178
Bme1390I CCNGG 4 cut(s) 85, 214, 2635, 2676
Bme18I GGWCC 1 cut(s) 2915
BmeRI GACNNNNNGTC 1 cut(s) 1342
BmeT110I CYCGRG 2 cut(s) 7, 2486
BmgBI CACGTC 1 cut(s) 3337
BmgT120I GGNCC 1 cut(s) 2915
BmiI GGNNCC 1 cut(s) 342
BmrFI CCNGG 4 cut(s) 85, 214, 2635, 2676
BmrI ACTGGG 2 cut(s) 1169, 3232
BmsI GCATC 5 cut(s) 162, 415, 1063, 1536, 1639
BmtI GCTAGC 1 cut(s) 2175
BmuI ACTGGG 2 cut(s) 1169, 3232
BpiI GAAGAC 7 cut(s) 84, 698, 1230, 1404, 2344, 3033, 3131
BpuEI CTTGAG 4 cut(s) 1271, 1964, 2046, 2504
BpuMI CCSGG 1 cut(s) 85
BsaAI YACGTR 1 cut(s) 2687
BsaBI GATNNNNATC 1 cut(s) 2711
BsaHI GRCGYC 1 cut(s) 1338
BsaI GGTCTC 1 cut(s) 1175
BsaJI CCNNGG 6 cut(s) 154, 699, 1026, 2467, 2500, 2886
Bsc4I CCNNNNNNNGG 6 cut(s) 733, 1027, 1279, 2488, 2977, 3208
Bse1I ACTGG 7 cut(s) 1175, 1232, 1834, 2417, 2982, 3162, 3238
Bse3DI GCAATG 3 cut(s) 424, 1578, 2811
Bse8I GATNNNNATC 1 cut(s) 2711
BseBI CCWGG 3 cut(s) 214, 2635, 2676
BseDI CCNNGG 6 cut(s) 154, 699, 1026, 2467, 2500, 2886
BseGI GGATG 8 cut(s) 154, 177, 274, 1386, 1525, 1551, 1654, 2917
BseJI GATNNNNATC 1 cut(s) 2711
BseLI CCNNNNNNNGG 6 cut(s) 733, 1027, 1279, 2488, 2977, 3208
BseMI GCAATG 3 cut(s) 424, 1578, 2811
BseMII CTCAG 5 cut(s) 53, 76, 381, 1680, 2855
BseNI ACTGG 7 cut(s) 1175, 1232, 1834, 2417, 2982, 3162, 3238
BseRI GAGGAG 4 cut(s) 27, 851, 1295, 2663
BseSI GKGCMC 4 cut(s) 721, 1204, 3203, 3236
BseYI CCCAGC 3 cut(s) 479, 817, 953
BsgI GTGCAG 2 cut(s) 240, 2253
Bsh1236I CGCG 1 cut(s) 2118
Bsh1285I CGRYCG 2 cut(s) 2313, 2500
BshFI GGCC 5 cut(s) 5, 470, 559, 1571, 1599
BsiEI CGRYCG 2 cut(s) 2313, 2500
BsiHKAI GWGCWC 3 cut(s) 14, 721, 1477
BsiHKCI CYCGRG 2 cut(s) 7, 2486
BsiSI CCGG 1 cut(s) 85
BslFI GGGAC 3 cut(s) 1537, 2288, 3323
BslI CCNNNNNNNGG 6 cut(s) 733, 1027, 1279, 2488, 2977, 3208
BsmBI CGTCTC 1 cut(s) 1954
BsmFI GGGAC 3 cut(s) 1537, 2288, 3323
BsmI GAATGC 3 cut(s) 1583, 2173, 2243
BsnI GGCC 5 cut(s) 5, 470, 559, 1571, 1599
Bso31I GGTCTC 1 cut(s) 1175
BsoBI CYCGRG 2 cut(s) 7, 2486
Bsp1286I GDGCHC 6 cut(s) 14, 721, 1204, 1477, 3203, 3236
Bsp1407I TGTACA 2 cut(s) 2097, 2293
Bsp19I CCATGG 4 cut(s) 699, 1026, 2467, 2886
Bsp68I TCGCGA 1 cut(s) 2118
BspACI CCGC 6 cut(s) 809, 1652, 2138, 2735, 2800, 3346
BspANI GGCC 5 cut(s) 5, 470, 559, 1571, 1599
BspCNI CTCAG 5 cut(s) 52, 75, 382, 1679, 2856
BspFNI CGCG 1 cut(s) 2118
BspHI TCATGA 1 cut(s) 2382
BspLI GGNNCC 1 cut(s) 342
BspMI ACCTGC 2 cut(s) 1273, 2206
BspOI GCTAGC 1 cut(s) 2175
BspPI GGATC 8 cut(s) 335, 348, 776, 819, 889, 1765, 2877, 3335
BspTI CTTAAG 1 cut(s) 2693
BspTNI GGTCTC 1 cut(s) 1175
BsrBI CCGCTC 1 cut(s) 3348
BsrDI GCAATG 3 cut(s) 424, 1578, 2811
BsrGI TGTACA 2 cut(s) 2097, 2293
BsrI ACTGG 7 cut(s) 1175, 1232, 1834, 2417, 2982, 3162, 3238
BssECI CCNNGG 6 cut(s) 154, 699, 1026, 2467, 2500, 2886
BssNI GRCGYC 1 cut(s) 1338
BssT1I CCWWGG 5 cut(s) 154, 699, 1026, 2467, 2886
Bst2UI CCWGG 3 cut(s) 214, 2635, 2676
Bst4CI ACNGT 7 cut(s) 1331, 2053, 2097, 2311, 2501, 2745, 3114
Bst6I CTCTTC 4 cut(s) 31, 393, 2613, 2993
BstACI GRCGYC 1 cut(s) 1338
BstAFI CTTAAG 1 cut(s) 2693
BstAPI GCANNNNNTGC 3 cut(s) 172, 1187, 1695
BstAUI TGTACA 2 cut(s) 2097, 2293
BstBAI YACGTR 1 cut(s) 2687
BstC8I GCNNGC 5 cut(s) 1700, 2173, 2243, 2802, 3232
BstDEI CTNAG 9 cut(s) 39, 62, 390, 907, 1666, 1813, 1820, 1952, 2864
BstDSI CCRYGG 5 cut(s) 699, 1026, 2467, 2500, 2886
BstENI CCTNNNNNAGG 2 cut(s) 1277, 2486
BstF5I GGATG 8 cut(s) 154, 177, 274, 1386, 1525, 1551, 1654, 2917
BstFNI CGCG 1 cut(s) 2118
BstMCI CGRYCG 2 cut(s) 2313, 2500
BstMWI GCNNNNNNNGC 6 cut(s) 172, 476, 1187, 1695, 1830, 3154
BstNI CCWGG 3 cut(s) 214, 2635, 2676
BstNSI RCATGY 2 cut(s) 713, 1442
BstSCI CCNGG 4 cut(s) 83, 212, 2633, 2674
BstSFI CTRYAG 1 cut(s) 1590
BstSLI GKGCMC 4 cut(s) 721, 1204, 3203, 3236
BstSNI TACGTA 1 cut(s) 2687
BstUI CGCG 1 cut(s) 2118
BstV2I GAAGAC 7 cut(s) 84, 698, 1230, 1404, 2344, 3033, 3131
BstX2I RGATCY 7 cut(s) 340, 781, 811, 894, 1770, 1816, 2882
BstXI CCANNNNNNTGG 1 cut(s) 1714
BstYI RGATCY 7 cut(s) 340, 781, 811, 894, 1770, 1816, 2882
BsuI GTATCC 2 cut(s) 109, 778
BsuRI GGCC 5 cut(s) 5, 470, 559, 1571, 1599
BtgI CCRYGG 5 cut(s) 699, 1026, 2467, 2500, 2886
BtrI CACGTC 1 cut(s) 3337
BtsCI GGATG 8 cut(s) 154, 177, 274, 1386, 1525, 1551, 1654, 2917
BtsI GCAGTG 2 cut(s) 311, 1841
BtsIMutI CAGTG 7 cut(s) 47, 311, 387, 1336, 1827, 1841, 2428
BtuMI TCGCGA 1 cut(s) 2118
BveI ACCTGC 2 cut(s) 1273, 2206
Cac8I GCNNGC 5 cut(s) 1700, 2173, 2243, 2802, 3232
CaiI CAGNNNCTG 1 cut(s) 2977
CciI TCATGA 1 cut(s) 2382
Cfr13I GGNCC 1 cut(s) 2915
CseI GACGC 2 cut(s) 1346, 1971
CsiI ACCWGGT 1 cut(s) 2633
Csp6I GTAC 6 cut(s) 554, 2098, 2294, 2455, 3165, 3177
CviQI GTAC 6 cut(s) 554, 2098, 2294, 2455, 3165, 3177
DdeI CTNAG 9 cut(s) 39, 62, 390, 907, 1666, 1813, 1820, 1952, 2864
DraI TTTAAA 2 cut(s) 2205, 3132
DraIII CACNNNGTG 1 cut(s) 2637
DrdI GACNNNNNNGTC 1 cut(s) 2587
DriI GACNNNNNGTC 1 cut(s) 1342
DseDI GACNNNNNNGTC 1 cut(s) 2587
EaeI YGGCCR 3 cut(s) 468, 1569, 1597
Eam1104I CTCTTC 4 cut(s) 31, 393, 2613, 2993
Eam1105I GACNNNNNGTC 1 cut(s) 1342
EarI CTCTTC 4 cut(s) 31, 393, 2613, 2993
Ecl136II GAGCTC 1 cut(s) 12
Eco105I TACGTA 1 cut(s) 2687
Eco130I CCWWGG 5 cut(s) 154, 699, 1026, 2467, 2886
Eco24I GRGCYC 1 cut(s) 14
Eco31I GGTCTC 1 cut(s) 1175
Eco32I GATATC 1 cut(s) 2070
Eco47I GGWCC 1 cut(s) 2915
Eco53kI GAGCTC 1 cut(s) 12
Eco57I CTGAAG 4 cut(s) 52, 146, 318, 1715
Eco88I CYCGRG 2 cut(s) 7, 2486
EcoICRI GAGCTC 1 cut(s) 12
EcoNI CCTNNNNNAGG 2 cut(s) 1277, 2486
EcoRI GAATTC 1 cut(s) 948
EcoRII CCWGG 3 cut(s) 212, 2633, 2674
EcoRV GATATC 1 cut(s) 2070
EcoT14I CCWWGG 5 cut(s) 154, 699, 1026, 2467, 2886
EcoT22I ATGCAT 1 cut(s) 1551
EcoT38I GRGCYC 1 cut(s) 14
ErhI CCWWGG 5 cut(s) 154, 699, 1026, 2467, 2886
Esp3I CGTCTC 1 cut(s) 1954
FalI AAGNNNNNCTT 6 cut(s) 789, 821, 1093, 1125, 1334, 1366
FaqI GGGAC 3 cut(s) 1537, 2288, 3323
FauI CCCGC 2 cut(s) 2131, 2793
FauNDI CATATG 1 cut(s) 2906
FbaI TGATCA 1 cut(s) 2379
FokI GGATG 8 cut(s) 161, 184, 281, 1373, 1532, 1558, 1661, 2924
FriOI GRGCYC 1 cut(s) 14
FspBI CTAG 7 cut(s) 861, 1122, 1155, 1488, 2172, 2583, 3287
GsaI CCCAGC 3 cut(s) 483, 821, 957
HaeIII GGCC 5 cut(s) 5, 470, 559, 1571, 1599
HapII CCGG 1 cut(s) 85
HgaI GACGC 2 cut(s) 1346, 1971
Hin1I GRCGYC 1 cut(s) 1338
HincII GTYRAC 1 cut(s) 1444
HindII GTYRAC 1 cut(s) 1444
HindIII AAGCTT 4 cut(s) 1500, 1700, 1878, 1940
HpaII CCGG 1 cut(s) 85
HphI GGTGA 8 cut(s) 86, 448, 715, 988, 1624, 2623, 2675, 2740
Hpy166II GTNNAC 3 cut(s) 719, 851, 1444
Hpy8I GTNNAC 3 cut(s) 719, 851, 1444
Hpy99I CGWCG 3 cut(s) 1340, 3335, 3338
HpyCH4III ACNGT 7 cut(s) 1331, 2053, 2097, 2311, 2501, 2745, 3114
HpyCH4IV ACGT 5 cut(s) 2253, 2686, 2847, 3030, 3336
HpyF10VI GCNNNNNNNGC 6 cut(s) 172, 476, 1187, 1695, 1830, 3154
HpyF3I CTNAG 9 cut(s) 39, 62, 390, 907, 1666, 1813, 1820, 1952, 2864
HpySE526I ACGT 5 cut(s) 2253, 2686, 2847, 3030, 3336
Hsp92I GRCGYC 1 cut(s) 1338
Ksp22I TGATCA 1 cut(s) 2379
LmnI GCTCC 3 cut(s) 17, 40, 2926
LweI GCATC 5 cut(s) 162, 415, 1063, 1536, 1639
MabI ACCWGGT 1 cut(s) 2633
MaeI CTAG 7 cut(s) 861, 1122, 1155, 1488, 2172, 2583, 3287
MaeII ACGT 5 cut(s) 2253, 2686, 2847, 3030, 3336
MaeIII GTNAC 5 cut(s) 1331, 1483, 1967, 2428, 3341
MbiI CCGCTC 1 cut(s) 3348
MfeI CAATTG 1 cut(s) 3020
MflI RGATCY 7 cut(s) 340, 781, 811, 894, 1770, 1816, 2882
MhlI GDGCHC 6 cut(s) 14, 721, 1204, 1477, 3203, 3236
MlsI TGGCCA 2 cut(s) 470, 1599
MluNI TGGCCA 2 cut(s) 470, 1599
MlyI GAGTC 7 cut(s) 848, 1476, 1866, 2026, 2511, 2779, 2950
MmeI TCCRAC 5 cut(s) 465, 547, 2004, 2604, 3307
Mox20I TGGCCA 2 cut(s) 470, 1599
Mph1103I ATGCAT 1 cut(s) 1551
MroXI GAANNNNTTC 1 cut(s) 2872
MscI TGGCCA 2 cut(s) 470, 1599
MslI CAYNNNNRTG 2 cut(s) 714, 2499
Msp20I TGGCCA 2 cut(s) 470, 1599
MspCI CTTAAG 1 cut(s) 2693
MspI CCGG 1 cut(s) 85
MspR9I CCNGG 4 cut(s) 85, 214, 2635, 2676
MssI GTTTAAAC 1 cut(s) 2205
MunI CAATTG 1 cut(s) 3020
Mva1269I GAATGC 3 cut(s) 1583, 2173, 2243
MvaI CCWGG 3 cut(s) 214, 2635, 2676
MvnI CGCG 1 cut(s) 2118
MwoI GCNNNNNNNGC 6 cut(s) 172, 476, 1187, 1695, 1830, 3154
NciI CCSGG 1 cut(s) 85
NcoI CCATGG 4 cut(s) 699, 1026, 2467, 2886
NdeI CATATG 1 cut(s) 2906
NheI GCTAGC 1 cut(s) 2171
NlaIV GGNNCC 1 cut(s) 342
NmeAIII GCCGAG 1 cut(s) 600
NmuCI GTSAC 2 cut(s) 1331, 2428
NruI TCGCGA 1 cut(s) 2118
NsiI ATGCAT 1 cut(s) 1551
NspI RCATGY 2 cut(s) 713, 1442
OliI CACNNNNGTG 1 cut(s) 2499
PaeR7I CTCGAG 2 cut(s) 7, 2486
PagI TCATGA 1 cut(s) 2382
PciI ACATGT 2 cut(s) 709, 1438
PcsI WCGNNNNNNNCGW 2 cut(s) 2310, 2493
PctI GAATGC 3 cut(s) 1583, 2173, 2243
PdmI GAANNNNTTC 1 cut(s) 2872
PfeI GAWTC 7 cut(s) 209, 283, 488, 599, 1948, 2537, 2931
PflFI GACNNNGTC 2 cut(s) 2309, 2348
PflMI CCANNNNNTGG 3 cut(s) 733, 1027, 2977
PleI GAGTC 7 cut(s) 847, 1475, 1866, 2026, 2511, 2778, 2949
PmeI GTTTAAAC 1 cut(s) 2205
PpsI GAGTC 7 cut(s) 847, 1475, 1866, 2026, 2511, 2778, 2949
Ppu21I YACGTR 1 cut(s) 2687
PscI ACATGT 2 cut(s) 709, 1438
PsiI TTATAA 2 cut(s) 333, 902
Psp124BI GAGCTC 1 cut(s) 14
Psp1406I AACGTT 1 cut(s) 2253
Psp6I CCWGG 3 cut(s) 212, 2633, 2674
PspFI CCCAGC 3 cut(s) 479, 817, 953
PspGI CCWGG 3 cut(s) 212, 2633, 2674
PspN4I GGNNCC 1 cut(s) 342
PspPI GGNCC 1 cut(s) 2915
PspXI VCTCGAGB 1 cut(s) 7
PstNI CAGNNNCTG 1 cut(s) 2977
PsuI RGATCY 7 cut(s) 340, 781, 811, 894, 1770, 1816, 2882
PsyI GACNNNGTC 2 cut(s) 2309, 2348
RruI TCGCGA 1 cut(s) 2118
RsaI GTAC 6 cut(s) 555, 2099, 2295, 2456, 3166, 3178
RsaNI GTAC 6 cut(s) 554, 2098, 2294, 2455, 3165, 3177
RseI CAYNNNNRTG 2 cut(s) 714, 2499
SacI GAGCTC 1 cut(s) 14
Sau96I GGNCC 1 cut(s) 2915
ScaI AGTACT 1 cut(s) 3178
SchI GAGTC 7 cut(s) 848, 1476, 1866, 2026, 2511, 2779, 2950
ScrFI CCNGG 4 cut(s) 85, 214, 2635, 2676
SduI GDGCHC 6 cut(s) 14, 721, 1204, 1477, 3203, 3236
SexAI ACCWGGT 1 cut(s) 2633
SfaNI GCATC 5 cut(s) 162, 415, 1063, 1536, 1639
SfcI CTRYAG 1 cut(s) 1590
Sfr274I CTCGAG 2 cut(s) 7, 2486
SinI GGWCC 1 cut(s) 2915
SlaI CTCGAG 2 cut(s) 7, 2486
SmiMI CAYNNNNRTG 2 cut(s) 714, 2499
SmlI CTYRAG 7 cut(s) 7, 1250, 1943, 2061, 2486, 2519, 2693
SmoI CTYRAG 7 cut(s) 7, 1250, 1943, 2061, 2486, 2519, 2693
SnaBI TACGTA 1 cut(s) 2687
SsiI CCGC 6 cut(s) 809, 1652, 2138, 2735, 2800, 3346
SspI AATATT 1 cut(s) 793
SspMI CTAG 7 cut(s) 861, 1122, 1155, 1488, 2172, 2583, 3287
SstI GAGCTC 1 cut(s) 14
StyD4I CCNGG 4 cut(s) 83, 212, 2633, 2674
StyI CCWWGG 5 cut(s) 154, 699, 1026, 2467, 2886
TaaI ACNGT 7 cut(s) 1331, 2053, 2097, 2311, 2501, 2745, 3114
TaiI ACGT 5 cut(s) 2256, 2689, 2850, 3033, 3339
TaqII GACCGA 1 cut(s) 2402
TatI WGTACW 4 cut(s) 2097, 2293, 3164, 3176
TfiI GAWTC 7 cut(s) 209, 283, 488, 599, 1948, 2537, 2931
TscAI CASTG 7 cut(s) 47, 318, 394, 1336, 1834, 1848, 2428
TseFI GTSAC 2 cut(s) 1331, 2428
Tsp45I GTSAC 2 cut(s) 1331, 2428
TspGWI ACGGA 2 cut(s) 1379, 3334
TspRI CASTG 7 cut(s) 47, 318, 394, 1336, 1834, 1848, 2428
Tth111I GACNNNGTC 2 cut(s) 2309, 2348
Van91I CCANNNNNTGG 3 cut(s) 733, 1027, 2977
Vha464I CTTAAG 1 cut(s) 2693
VneI GTGCAC 1 cut(s) 717
VpaK11BI GGWCC 1 cut(s) 2915
XagI CCTNNNNNAGG 2 cut(s) 1277, 2486
XapI RAATTY 7 cut(s) 497, 593, 948, 1044, 1982, 2002, 2011
XceI RCATGY 2 cut(s) 713, 1442
XhoI CTCGAG 2 cut(s) 7, 2486
XmnI GAANNNNTTC 1 cut(s) 2872
XspI CTAG 7 cut(s) 861, 1122, 1155, 1488, 2172, 2583, 3287
ZrmI AGTACT 1 cut(s) 3178
Zsp2I ATGCAT 1 cut(s) 1551
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.