Rorug03G0271000
ERF Family

Protein kinase superfamily protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0010054
Physical Location & Seq
Forward (+)
26726078 .. 26729531
3454 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0271000.1

Sequence Viewer

Length: 2049 bp
ATGACTTCTAACAATGAACAGCCTGCAAATACTATTACTATGAATTCCAGCTCTGATTCTTTGGGACTTGCAGCTGGATTCACTGAAAAGAAGTTTACGTCAAGTAATAGTAGTGCAGAAAGCAGTAGGCAGTGTGAGGCAAAAGGTCGAGGTGTAGCTCCTGAAGAAAAGGAATCATCATGTATGATCAACAACAAACAACTTGCACAAACTAGTATCATGGGCTCCAGCCCAGATTCTTTGAAACTTACACCTGGCTACATTGACAAGAAAACAACAAGCAGTTGTGATTCAGAAAGCACTAGGCCATGGGAGGCCAAAGGACAAGGTTTTCTTACTGAAGAAAAGGAAATGATTCCAACTGCATCTTTGCACTATGAAGGAAATCGTATGCAAAATTCAGAAGTTAAGTGTATGAGTACAAAATATTCAACTAGTGTCTGCTCCATTGATGAGAAGACAAGCCAAGAAAGTTTTGATTCTAAATTGATTGATGTGGTGGTAGAGCACTCTTTGATACCCACAGCAGATAATATAGGTACTTCACAGAGGCCTGATGGACATGAATCAGATGTCAGTAAAACGGTTGAGCCCCCAGATGTTATTGCCAGCGATTCTCTTATATATGGAAATGGCTGTTCCAGTGAGGTGAAAAATTCATGCATGCCTATTTCGACAGAAAATGGATGTGATGAAATAAGGAAACAGCTTTCTACTGCTACAGCTAGAAGTCAAACCTTGATGTCATCTTTAACTCCTGAAATCAAGGCAACCCTTCTAAATACCAAAAAGCCTGCATGTTCCCTTGTTAAGTATGGTGGTGAAGATACTTTGAGAGAATCAGCTGGCATCAAGAAGAGTAATGAATGTTTGGAATCCAGATTGTGTGCTTTAAGCCCGACAAAAGGTGATGTAGAGCAAAAGCTAATTGACTTTGTTCCGCAAACTGCACTAAAAGTGGGCATTAAAGAATCAAAGATGACTCTGGGTAACAAGATTGGCTGTCCAAATAAGTCTTTAAAGACTGCTGGGAATGGAAGAAATCATCGACAAACAGTCAAGTTTCAAGGCCGGATCACTGATGAGGAAGATTCTGATCAGTTTGGAACAGCAAAAGAAACTCAAAAGGATGAGAATGCTGATCTTGATGGTAAGCCCCTCTTGCCCTTGGGTTTGAAACCACAAAGGCCAAAGCGAAAGAGGAAGTTACCGTTGACTGTGCAGAATGTCCAAGAAACATGTTTTCCTTCTACAGAAATGAGTTCAAATACGACGTTTATTTCAAAAGCTCAAAAGGAACAAAAATCCAAAGTAATTGATGACTATACTAGGTTAAATCAGTTTTTTGGTTCGAAGCAGTGTGAAAAATCAGACCATCTTGAACAATATGAAGACAAAGAACATGCCACTTCTCTTGGTAACAGTAAAACTTCAGCTTCACAGTTGCAAAAGAAGAGGAAAAGGACTCCAAGTTTCCAATTTACTGCAGACACTGAAGAATGCACTGGATATCTAGGCTCGAAAGATTTACACAGGGATGTGCAAAAGAAGAGGAAAGGGACGTCAACTTTGACACGTCCTGCAGAGGTTAAAGATTTGTCCTTTTTGGGATTGCGAGATTCACACACAGACGTAACTAATGGTTCTATTTGGGAAGATCTCGAGCATCCAGAAAAGGAAGATCCAAAAATTGTAGAGTCTTCAAAGTCACAAAAGCAGAGGAAAATGACTTCAACTTTCCCACTTCCTGCAGAAAGTAATGATTTGCCTTTAGGTTTGGGCTTGCAAGATTTACACAAGGACAGGGACACAACTAATGGTTGGACTGAGGAAGATCCTCTGATCGTAAAGTCCTATTCTGGAGATGATTCTCAAGCTAGGAATTTGGTTCCGTATGCAATTCCAACTCCTATTCCAAGTCTGAGGTTGAAGGAAATGACAGTAGGTCAACTGAAGGAATTGGCAAAGAGGCTCGAAGTGAAAGGCTTTTCCAAGCTTAAGAAAATGGAGCTGGTTGAGCTACTAGCCAGGAAACAGAGAGGTCACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

682

Amino Acids

75.34

Weight (kDa)

7.78

Isoelectric Point (pI)

48.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rho_N PF07498 643 - 675 4.8e-06 Rho termination factor, N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1565
AciI CCGC 1 cut(s) 941
AclWI GGATC 3 cut(s) 1082, 1676, 1829
AcsI RAATTY 4 cut(s) 43, 397, 655, 1882
AcuI CTGAAG 5 cut(s) 183, 360, 1416, 1515, 1973
AcyI GRCGYC 1 cut(s) 1562
AfaI GTAC 2 cut(s) 421, 541
AfiI CCNNNNNNNGG 1 cut(s) 905
AflII CTTAAG 1 cut(s) 1997
AflIII ACRYGT 2 cut(s) 1238, 1574
AhdI GACNNNNNGTC 2 cut(s) 1055, 1944
AhlI ACTAGT 2 cut(s) 212, 434
AjiI CACGTC 1 cut(s) 1577
AjnI CCWGG 2 cut(s) 253, 2027
Alw21I GWGCWC 1 cut(s) 510
AlwI GGATC 3 cut(s) 1082, 1676, 1829
AlwNI CAGNNNCTG 1 cut(s) 1493
Ama87I CYCGRG 1 cut(s) 1661
AoxI GGCC 5 cut(s) 305, 315, 551, 1069, 1187
ApeKI GCWGC 1 cut(s) 71
ApoI RAATTY 4 cut(s) 43, 397, 655, 1882
ArsI GACNNNNNNTTYG 2 cut(s) 1552, 1584
Asp700I GAANNNNTTC 1 cut(s) 354
AsuHPI GGTGA 3 cut(s) 661, 833, 920
AsuII TTCGAA 1 cut(s) 1352
AvaI CYCGRG 1 cut(s) 1661
BanII GRGCYC 2 cut(s) 227, 594
BbsI GAAGAC 3 cut(s) 464, 1398, 1692
Bbv12I GWGCWC 1 cut(s) 510
BbvI GCAGC 1 cut(s) 83
BccI CCATC 3 cut(s) 551, 1142, 1383
BciT130I CCWGG 2 cut(s) 255, 2029
BclI TGATCA 2 cut(s) 186, 1096
BcuI ACTAGT 2 cut(s) 212, 434
BfaI CTAG 8 cut(s) 213, 303, 435, 726, 1329, 1514, 1878, 2024
BfmI CTRYAG 5 cut(s) 720, 1251, 1485, 1581, 1749
BfrI CTTAAG 1 cut(s) 1997
BglII AGATCT 1 cut(s) 1657
BisI GCNGC 1 cut(s) 72
BlsI GCNGC 1 cut(s) 73
Bme1390I CCNGG 2 cut(s) 255, 2029
BmeRI GACNNNNNGTC 2 cut(s) 1055, 1944
BmeT110I CYCGRG 1 cut(s) 1661
BmgBI CACGTC 1 cut(s) 1577
BmiI GGNNCC 2 cut(s) 226, 1890
BmrFI CCNGG 2 cut(s) 255, 2029
BmsI GCATC 3 cut(s) 374, 858, 1675
BpiI GAAGAC 3 cut(s) 464, 1398, 1692
BpmI CTGGAG 2 cut(s) 211, 1881
Bpu14I TTCGAA 1 cut(s) 1352
BpuEI CTTGAG 1 cut(s) 1857
BsaBI GATNNNNATC 1 cut(s) 1095
BsaHI GRCGYC 1 cut(s) 1562
BsaJI CCNNGG 2 cut(s) 308, 1167
Bsc4I CCNNNNNNNGG 1 cut(s) 905
Bse1I ACTGG 2 cut(s) 642, 1510
Bse8I GATNNNNATC 1 cut(s) 1095
BseBI CCWGG 2 cut(s) 255, 2029
BseDI CCNNGG 2 cut(s) 308, 1167
BseGI GGATG 4 cut(s) 692, 1135, 1543, 1666
BseJI GATNNNNATC 1 cut(s) 1095
BseLI CCNNNNNNNGG 1 cut(s) 905
BseMII CTCAG 2 cut(s) 1818, 1913
BseNI ACTGG 2 cut(s) 642, 1510
BseXI GCAGC 1 cut(s) 83
BseYI CCCAGC 1 cut(s) 1028
BsgI GTGCAG 3 cut(s) 135, 933, 1241
BshFI GGCC 5 cut(s) 307, 317, 553, 1071, 1189
BsiHKAI GWGCWC 1 cut(s) 510
BsiHKCI CYCGRG 1 cut(s) 1661
BsiSI CCGG 1 cut(s) 1072
BslFI GGGAC 3 cut(s) 78, 1573, 1820
BslI CCNNNNNNNGG 1 cut(s) 905
BsmFI GGGAC 3 cut(s) 78, 1573, 1820
BsmI GAATGC 2 cut(s) 1141, 1505
BsnI GGCC 5 cut(s) 307, 317, 553, 1071, 1189
BsoBI CYCGRG 1 cut(s) 1661
Bsp119I TTCGAA 1 cut(s) 1352
Bsp1286I GDGCHC 3 cut(s) 227, 510, 594
Bsp143I GATC 8 cut(s) 186, 1074, 1096, 1141, 1657, 1681, 1834, 1842
Bsp19I CCATGG 1 cut(s) 308
BspACI CCGC 1 cut(s) 941
BspANI GGCC 5 cut(s) 307, 317, 553, 1071, 1189
BspCNI CTCAG 2 cut(s) 1819, 1914
BspLI GGNNCC 2 cut(s) 226, 1890
BspMAI CTGCAG 3 cut(s) 1489, 1585, 1753
BspPI GGATC 3 cut(s) 1082, 1676, 1829
BspT104I TTCGAA 1 cut(s) 1352
BspTI CTTAAG 1 cut(s) 1997
BsrI ACTGG 2 cut(s) 642, 1510
BssECI CCNNGG 2 cut(s) 308, 1167
BssMI GATC 8 cut(s) 186, 1074, 1096, 1141, 1657, 1681, 1834, 1842
BssNI GRCGYC 1 cut(s) 1562
BssT1I CCWWGG 2 cut(s) 308, 1167
Bst2UI CCWGG 2 cut(s) 255, 2029
Bst4CI ACNGT 7 cut(s) 586, 1057, 1212, 1219, 1424, 1443, 1942
Bst6I CTCTTC 3 cut(s) 851, 1448, 1544
BstACI GRCGYC 1 cut(s) 1562
BstAFI CTTAAG 1 cut(s) 1997
BstBI TTCGAA 1 cut(s) 1352
BstC8I GCNNGC 6 cut(s) 24, 610, 665, 795, 847, 1784
BstDEI CTNAG 2 cut(s) 1827, 1922
BstDSI CCRYGG 1 cut(s) 308
BstF5I GGATG 4 cut(s) 692, 1135, 1543, 1666
BstKTI GATC 8 cut(s) 189, 1077, 1099, 1144, 1660, 1684, 1837, 1845
BstMBI GATC 8 cut(s) 186, 1074, 1096, 1141, 1657, 1681, 1834, 1842
BstMWI GCNNNNNNNGC 2 cut(s) 1162, 2017
BstNI CCWGG 2 cut(s) 255, 2029
BstNSI RCATGY 4 cut(s) 667, 801, 1242, 1406
BstSCI CCNGG 2 cut(s) 253, 2027
BstSFI CTRYAG 5 cut(s) 720, 1251, 1485, 1581, 1749
BstV1I GCAGC 1 cut(s) 83
BstV2I GAAGAC 3 cut(s) 464, 1398, 1692
BstX2I RGATCY 3 cut(s) 1657, 1681, 1834
BstYI RGATCY 3 cut(s) 1657, 1681, 1834
BsuRI GGCC 5 cut(s) 307, 317, 553, 1071, 1189
BtgI CCRYGG 1 cut(s) 308
BtrI CACGTC 1 cut(s) 1577
BtsCI GGATG 4 cut(s) 692, 1135, 1543, 1666
BtsI GCAGTG 2 cut(s) 137, 1364
BtsIMutI CAGTG 7 cut(s) 81, 137, 649, 1077, 1364, 1491, 1503
Cac8I GCNNGC 6 cut(s) 24, 610, 665, 795, 847, 1784
CaiI CAGNNNCTG 1 cut(s) 1493
Csp6I GTAC 2 cut(s) 420, 540
CspCI CAANNNNNGTGG 2 cut(s) 1396, 1431
CviAII CATG 9 cut(s) 180, 220, 309, 563, 660, 664, 798, 1239, 1403
CviQI GTAC 2 cut(s) 420, 540
DdeI CTNAG 2 cut(s) 1827, 1922
DpnI GATC 8 cut(s) 188, 1076, 1098, 1143, 1659, 1683, 1836, 1844
DpnII GATC 8 cut(s) 186, 1074, 1096, 1141, 1657, 1681, 1834, 1842
DraI TTTAAA 1 cut(s) 1020
DriI GACNNNNNGTC 2 cut(s) 1055, 1944
Eam1104I CTCTTC 3 cut(s) 851, 1448, 1544
Eam1105I GACNNNNNGTC 2 cut(s) 1055, 1944
EarI CTCTTC 3 cut(s) 851, 1448, 1544
Eco130I CCWWGG 2 cut(s) 308, 1167
Eco147I AGGCCT 1 cut(s) 553
Eco24I GRGCYC 2 cut(s) 227, 594
Eco32I GATATC 1 cut(s) 1511
Eco57I CTGAAG 5 cut(s) 183, 360, 1416, 1515, 1973
Eco88I CYCGRG 1 cut(s) 1661
EcoRI GAATTC 1 cut(s) 43
EcoRII CCWGG 2 cut(s) 253, 2027
EcoRV GATATC 1 cut(s) 1511
EcoT14I CCWWGG 2 cut(s) 308, 1167
EcoT22I ATGCAT 1 cut(s) 665
EcoT38I GRGCYC 2 cut(s) 227, 594
ErhI CCWWGG 2 cut(s) 308, 1167
FaeI CATG 9 cut(s) 183, 223, 312, 566, 663, 667, 801, 1242, 1406
FalI AAGNNNNNCTT 4 cut(s) 318, 350, 1145, 1177
FaqI GGGAC 3 cut(s) 78, 1573, 1820
FatI CATG 9 cut(s) 179, 219, 308, 562, 659, 663, 797, 1238, 1402
FbaI TGATCA 2 cut(s) 186, 1096
Fnu4HI GCNGC 1 cut(s) 72
FokI GGATG 4 cut(s) 699, 1142, 1550, 1653
FriOI GRGCYC 2 cut(s) 227, 594
Fsp4HI GCNGC 1 cut(s) 72
FspBI CTAG 8 cut(s) 213, 303, 435, 726, 1329, 1514, 1878, 2024
GluI GCNGC 1 cut(s) 72
GsaI CCCAGC 1 cut(s) 1032
GsuI CTGGAG 2 cut(s) 211, 1881
HaeIII GGCC 5 cut(s) 307, 317, 553, 1071, 1189
HapII CCGG 1 cut(s) 1072
Hin1I GRCGYC 1 cut(s) 1562
Hin1II CATG 9 cut(s) 183, 223, 312, 566, 663, 667, 801, 1242, 1406
HincII GTYRAC 3 cut(s) 1215, 1566, 1949
HindII GTYRAC 3 cut(s) 1215, 1566, 1949
HindIII AAGCTT 1 cut(s) 1994
HpaII CCGG 1 cut(s) 1072
HphI GGTGA 3 cut(s) 661, 833, 920
Hpy166II GTNNAC 4 cut(s) 96, 1215, 1566, 1949
Hpy188I TCNGA 8 cut(s) 55, 295, 403, 571, 1096, 1372, 1842, 1923
Hpy188III TCNNGA 9 cut(s) 161, 758, 853, 879, 1145, 1379, 1661, 1670, 1860
Hpy8I GTNNAC 4 cut(s) 96, 1215, 1566, 1949
Hpy99I CGWCG 1 cut(s) 1276
HpyAV CCTTC 5 cut(s) 374, 785, 1257, 1924, 1948
HpyCH4III ACNGT 7 cut(s) 586, 1057, 1212, 1219, 1424, 1443, 1942
HpyCH4IV ACGT 5 cut(s) 98, 1274, 1562, 1576, 1632
HpyF10VI GCNNNNNNNGC 2 cut(s) 1162, 2017
HpyF3I CTNAG 2 cut(s) 1827, 1922
HpySE526I ACGT 5 cut(s) 98, 1274, 1562, 1576, 1632
Hsp92I GRCGYC 1 cut(s) 1562
Hsp92II CATG 9 cut(s) 183, 223, 312, 566, 663, 667, 801, 1242, 1406
Ksp22I TGATCA 2 cut(s) 186, 1096
Kzo9I GATC 8 cut(s) 186, 1074, 1096, 1141, 1657, 1681, 1834, 1842
LmnI GCTCC 4 cut(s) 163, 230, 449, 2008
Lsp1109I GCAGC 1 cut(s) 83
LweI GCATC 3 cut(s) 374, 858, 1675
MaeI CTAG 8 cut(s) 213, 303, 435, 726, 1329, 1514, 1878, 2024
MaeII ACGT 5 cut(s) 98, 1274, 1562, 1576, 1632
MaeIII GTNAC 6 cut(s) 989, 1206, 1418, 1633, 1707, 2042
MalI GATC 8 cut(s) 188, 1076, 1098, 1143, 1659, 1683, 1836, 1844
MboI GATC 8 cut(s) 186, 1074, 1096, 1141, 1657, 1681, 1834, 1842
MflI RGATCY 3 cut(s) 1657, 1681, 1834
MhlI GDGCHC 3 cut(s) 227, 510, 594
MlyI GAGTC 3 cut(s) 976, 1459, 1706
MmeI TCCRAC 3 cut(s) 383, 1802, 1928
Mph1103I ATGCAT 1 cut(s) 665
MroXI GAANNNNTTC 1 cut(s) 354
MseI TTAA 9 cut(s) 408, 752, 810, 893, 966, 1019, 1334, 1590, 1998
MslI CAYNNNNRTG 1 cut(s) 1536
MspA1I CMGCKG 2 cut(s) 74, 845
MspCI CTTAAG 1 cut(s) 1997
MspI CCGG 1 cut(s) 1072
MspR9I CCNGG 2 cut(s) 255, 2029
Mva1269I GAATGC 2 cut(s) 1141, 1505
MvaI CCWGG 2 cut(s) 255, 2029
MwoI GCNNNNNNNGC 2 cut(s) 1162, 2017
NcoI CCATGG 1 cut(s) 308
NdeII GATC 8 cut(s) 186, 1074, 1096, 1141, 1657, 1681, 1834, 1842
NlaIII CATG 9 cut(s) 183, 223, 312, 566, 663, 667, 801, 1242, 1406
NlaIV GGNNCC 2 cut(s) 226, 1890
NmuCI GTSAC 2 cut(s) 1707, 2042
NsiI ATGCAT 1 cut(s) 665
NspI RCATGY 4 cut(s) 667, 801, 1242, 1406
NspV TTCGAA 1 cut(s) 1352
PaeI GCATGC 1 cut(s) 667
PaeR7I CTCGAG 1 cut(s) 1661
PceI AGGCCT 1 cut(s) 553
PciI ACATGT 1 cut(s) 1238
PctI GAATGC 2 cut(s) 1141, 1505
PdmI GAANNNNTTC 1 cut(s) 354
PkrI GCNGC 1 cut(s) 73
PleI GAGTC 3 cut(s) 976, 1459, 1705
PpsI GAGTC 3 cut(s) 976, 1459, 1705
PscI ACATGT 1 cut(s) 1238
Psp6I CCWGG 2 cut(s) 253, 2027
PspFI CCCAGC 1 cut(s) 1028
PspGI CCWGG 2 cut(s) 253, 2027
PspN4I GGNNCC 2 cut(s) 226, 1890
PstI CTGCAG 3 cut(s) 1489, 1585, 1753
PstNI CAGNNNCTG 1 cut(s) 1493
PsuI RGATCY 3 cut(s) 1657, 1681, 1834
PvuII CAGCTG 2 cut(s) 74, 845
RsaI GTAC 2 cut(s) 421, 541
RsaNI GTAC 2 cut(s) 420, 540
RseI CAYNNNNRTG 1 cut(s) 1536
SaqAI TTAA 9 cut(s) 408, 752, 810, 893, 966, 1019, 1334, 1590, 1998
SatI GCNGC 1 cut(s) 72
Sau3AI GATC 8 cut(s) 186, 1074, 1096, 1141, 1657, 1681, 1834, 1842
SchI GAGTC 3 cut(s) 976, 1459, 1706
ScrFI CCNGG 2 cut(s) 255, 2029
SduI GDGCHC 3 cut(s) 227, 510, 594
SfaNI GCATC 3 cut(s) 374, 858, 1675
SfcI CTRYAG 5 cut(s) 720, 1251, 1485, 1581, 1749
Sfr274I CTCGAG 1 cut(s) 1661
SfuI TTCGAA 1 cut(s) 1352
SlaI CTCGAG 1 cut(s) 1661
SmiMI CAYNNNNRTG 1 cut(s) 1536
SmlI CTYRAG 3 cut(s) 1661, 1872, 1997
SmoI CTYRAG 3 cut(s) 1661, 1872, 1997
SpeI ACTAGT 2 cut(s) 212, 434
SphI GCATGC 1 cut(s) 667
SseBI AGGCCT 1 cut(s) 553
SsiI CCGC 1 cut(s) 941
SspI AATATT 1 cut(s) 428
SspMI CTAG 8 cut(s) 213, 303, 435, 726, 1329, 1514, 1878, 2024
StuI AGGCCT 1 cut(s) 553
StyD4I CCNGG 2 cut(s) 253, 2027
StyI CCWWGG 2 cut(s) 308, 1167
TaaI ACNGT 7 cut(s) 586, 1057, 1212, 1219, 1424, 1443, 1942
TaiI ACGT 5 cut(s) 101, 1277, 1565, 1579, 1635
TaqI TCGA 7 cut(s) 148, 674, 1048, 1352, 1520, 1662, 1974
TatI WGTACW 1 cut(s) 419
Tru1I TTAA 9 cut(s) 408, 752, 810, 893, 966, 1019, 1334, 1590, 1998
Tru9I TTAA 9 cut(s) 408, 752, 810, 893, 966, 1019, 1334, 1590, 1998
TscAI CASTG 7 cut(s) 88, 137, 649, 1084, 1364, 1498, 1510
TseFI GTSAC 2 cut(s) 1707, 2042
TseI GCWGC 1 cut(s) 71
Tsp45I GTSAC 2 cut(s) 1707, 2042
TspDTI ATGAA 8 cut(s) 30, 56, 393, 579, 648, 708, 879, 1404
TspGWI ACGGA 1 cut(s) 1881
TspRI CASTG 7 cut(s) 88, 137, 649, 1084, 1364, 1498, 1510
Vha464I CTTAAG 1 cut(s) 1997
XapI RAATTY 4 cut(s) 43, 397, 655, 1882
XceI RCATGY 4 cut(s) 667, 801, 1242, 1406
XhoI CTCGAG 1 cut(s) 1661
XmnI GAANNNNTTC 1 cut(s) 354
XspI CTAG 8 cut(s) 213, 303, 435, 726, 1329, 1514, 1878, 2024
ZraI GACGTC 1 cut(s) 1563
Zsp2I ATGCAT 1 cut(s) 665
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.