Rorug04G0158100

F-box LRR-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0008069
Physical Location & Seq
Forward (+)
27568427 .. 27570816
2390 bp
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UTR
Exon/CDS
Intron
Rorug04G0158100.1

Sequence Viewer

Length: 501 bp
ATGAAGAGGTCACATCCATCAAATGACCAGGTTGATGTTATATCATCAGATGACTCGTCTTCCTCAGATGCAGAGATGGAAGTCACTACTGAGTATGTTAGCAAGCAGTTAACATCTGAAGGTGCTCTGATGAGAAGGGCAGAGATGTATCAGGAGTACATGAAGCACATCCCAATCCCAACACACCGTGGCTCTGTTATTCCATTTACCTCATGGATGGGTTTAGGTAAATCCATTAAGCAGCTTTATGGACAACCTCTGCATTATCTCACCAATATTCAGCTAAAACAGTGGGACCAATTGAGGATCGGCAGTGAAGACGAATACAGGCCCTTGGATGCCATTATTCACCCCTGTAAAGCCGAAGCCACCATCTGGCTCATTGAAGAAGTTCACCGAAACACCTCATCTCATCATCATGTAGCCAATCTGTGGCTATCAGATCCGATGCACCATGCTTTTGTTGATTCCATCTTTCCCCAATTAAACAGCAGCTCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box-like LRR_6

Protein Analysis

166

Amino Acids

19.04

Weight (kDa)

5.83

Isoelectric Point (pI)

62.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RdDM_RDM1 PF09187 43 - 161 1e-68 RNA-directed DNA methylation 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 375, 432
AclWI GGATC 2 cut(s) 314, 437
AcuI CTGAAG 1 cut(s) 138
AdeI CACNNNGTG 1 cut(s) 188
AfaI GTAC 1 cut(s) 158
AfiI CCNNNNNNNGG 2 cut(s) 375, 432
AgsI TTSAA 1 cut(s) 386
AjnI CCWGG 1 cut(s) 27
AluBI AGCT 3 cut(s) 244, 283, 495
AluI AGCT 3 cut(s) 244, 283, 495
Alw21I GWGCWC 1 cut(s) 127
AlwI GGATC 2 cut(s) 314, 437
AoxI GGCC 1 cut(s) 329
ApeKI GCWGC 2 cut(s) 241, 492
Asp700I GAANNNNTTC 1 cut(s) 390
AspS9I GGNCC 2 cut(s) 295, 330
AsuHPI GGTGA 3 cut(s) 262, 341, 386
AvaII GGWCC 1 cut(s) 295
BauI CACGAG 1 cut(s) 496
BbsI GAAGAC 2 cut(s) 51, 324
Bbv12I GWGCWC 1 cut(s) 127
BbvI GCAGC 1 cut(s) 253
BccI CCATC 5 cut(s) 25, 70, 211, 380, 479
BciT130I CCWGG 1 cut(s) 29
BisI GCNGC 2 cut(s) 242, 493
BlsI GCNGC 2 cut(s) 243, 494
Bme1390I CCNGG 1 cut(s) 29
Bme18I GGWCC 1 cut(s) 295
BmgT120I GGNCC 2 cut(s) 295, 330
BmiI GGNNCC 1 cut(s) 296
BmrFI CCNGG 1 cut(s) 29
BmsI GCATC 3 cut(s) 58, 328, 438
BpiI GAAGAC 2 cut(s) 51, 324
BsaJI CCNNGG 2 cut(s) 187, 333
Bsc4I CCNNNNNNNGG 2 cut(s) 375, 432
BseBI CCWGG 1 cut(s) 29
BseDI CCNNGG 2 cut(s) 187, 333
BseGI GGATG 4 cut(s) 13, 168, 222, 343
BseLI CCNNNNNNNGG 2 cut(s) 375, 432
BseMII CTCAG 2 cut(s) 78, 81
BseXI GCAGC 1 cut(s) 253
BshFI GGCC 1 cut(s) 331
BsiHKAI GWGCWC 1 cut(s) 127
BslFI GGGAC 1 cut(s) 308
BslI CCNNNNNNNGG 2 cut(s) 375, 432
BsmFI GGGAC 1 cut(s) 308
BsnI GGCC 1 cut(s) 331
Bsp1286I GDGCHC 1 cut(s) 127
Bsp143I GATC 2 cut(s) 306, 442
BspANI GGCC 1 cut(s) 331
BspCNI CTCAG 2 cut(s) 77, 82
BspLI GGNNCC 1 cut(s) 296
BspPI GGATC 2 cut(s) 314, 437
BssECI CCNNGG 2 cut(s) 187, 333
BssMI GATC 2 cut(s) 306, 442
BssSI CACGAG 1 cut(s) 496
BssT1I CCWWGG 1 cut(s) 333
Bst2BI CACGAG 1 cut(s) 496
Bst2UI CCWGG 1 cut(s) 29
Bst4CI ACNGT 2 cut(s) 188, 291
BstC8I GCNNGC 1 cut(s) 104
BstDEI CTNAG 2 cut(s) 64, 90
BstDSI CCRYGG 1 cut(s) 187
BstF5I GGATG 4 cut(s) 13, 168, 222, 343
BstKTI GATC 2 cut(s) 309, 445
BstMBI GATC 2 cut(s) 306, 442
BstNI CCWGG 1 cut(s) 29
BstSCI CCNGG 1 cut(s) 27
BstV1I GCAGC 1 cut(s) 253
BstV2I GAAGAC 2 cut(s) 51, 324
BstX2I RGATCY 1 cut(s) 442
BstYI RGATCY 1 cut(s) 442
BsuRI GGCC 1 cut(s) 331
BtgI CCRYGG 1 cut(s) 187
BtsCI GGATG 4 cut(s) 13, 168, 222, 343
BtsI GCAGTG 1 cut(s) 319
BtsIMutI CAGTG 2 cut(s) 296, 319
Cac8I GCNNGC 1 cut(s) 104
Cfr13I GGNCC 2 cut(s) 295, 330
CsiI ACCWGGT 1 cut(s) 27
Csp6I GTAC 1 cut(s) 157
CviAII CATG 4 cut(s) 160, 213, 419, 455
CviQI GTAC 1 cut(s) 157
DdeI CTNAG 2 cut(s) 64, 90
DpnI GATC 2 cut(s) 308, 444
DpnII GATC 2 cut(s) 306, 442
DraIII CACNNNGTG 1 cut(s) 188
Eco130I CCWWGG 1 cut(s) 333
Eco47I GGWCC 1 cut(s) 295
Eco57I CTGAAG 1 cut(s) 138
EcoO109I RGGNCCY 1 cut(s) 330
EcoRII CCWGG 1 cut(s) 27
EcoT14I CCWWGG 1 cut(s) 333
ErhI CCWWGG 1 cut(s) 333
FaeI CATG 4 cut(s) 163, 216, 422, 458
FaiI YATR 7 cut(s) 41, 96, 161, 214, 249, 420, 456
FaqI GGGAC 1 cut(s) 308
FatI CATG 4 cut(s) 159, 212, 418, 454
Fnu4HI GCNGC 2 cut(s) 242, 493
FokI GGATG 3 cut(s) 155, 229, 350
Fsp4HI GCNGC 2 cut(s) 242, 493
GluI GCNGC 2 cut(s) 242, 493
HaeIII GGCC 1 cut(s) 331
Hin1II CATG 4 cut(s) 163, 216, 422, 458
HincII GTYRAC 1 cut(s) 111
HindII GTYRAC 1 cut(s) 111
HinfI GANTC 2 cut(s) 53, 467
HpaI GTTAAC 1 cut(s) 111
HphI GGTGA 3 cut(s) 262, 341, 386
Hpy166II GTNNAC 2 cut(s) 111, 394
Hpy188I TCNGA 6 cut(s) 49, 67, 118, 129, 442, 447
Hpy188III TCNNGA 2 cut(s) 152, 498
Hpy8I GTNNAC 2 cut(s) 111, 394
HpyAV CCTTC 2 cut(s) 113, 129
HpyCH4III ACNGT 2 cut(s) 188, 291
HpyCH4V TGCA 3 cut(s) 71, 262, 451
HpyF3I CTNAG 2 cut(s) 64, 90
Hsp92II CATG 4 cut(s) 163, 216, 422, 458
KspAI GTTAAC 1 cut(s) 111
Kzo9I GATC 2 cut(s) 306, 442
LpnPI CCDG 6 cut(s) 14, 41, 137, 313, 361, 367
Lsp1109I GCAGC 1 cut(s) 253
LweI GCATC 3 cut(s) 58, 328, 438
MabI ACCWGGT 1 cut(s) 27
MaeIII GTNAC 2 cut(s) 9, 82
MalI GATC 2 cut(s) 308, 444
MboI GATC 2 cut(s) 306, 442
MboII GAAGA 4 cut(s) 16, 51, 329, 398
MfeI CAATTG 1 cut(s) 299
MflI RGATCY 1 cut(s) 442
MhlI GDGCHC 1 cut(s) 127
MluCI AATT 2 cut(s) 299, 482
MlyI GAGTC 1 cut(s) 47
MnlI CCTC 5 cut(s) 73, 220, 267, 297, 415
MroXI GAANNNNTTC 1 cut(s) 390
MseI TTAA 3 cut(s) 110, 237, 485
MslI CAYNNNNRTG 1 cut(s) 417
MspR9I CCNGG 1 cut(s) 29
MunI CAATTG 1 cut(s) 299
MvaI CCWGG 1 cut(s) 29
NdeII GATC 2 cut(s) 306, 442
NlaIII CATG 4 cut(s) 163, 216, 422, 458
NlaIV GGNNCC 1 cut(s) 296
NmuCI GTSAC 2 cut(s) 9, 82
PdmI GAANNNNTTC 1 cut(s) 390
PfeI GAWTC 1 cut(s) 467
PflMI CCANNNNNTGG 2 cut(s) 375, 432
PkrI GCNGC 2 cut(s) 243, 494
PleI GAGTC 1 cut(s) 47
PpsI GAGTC 1 cut(s) 47
Psp6I CCWGG 1 cut(s) 27
PspGI CCWGG 1 cut(s) 27
PspN4I GGNNCC 1 cut(s) 296
PspPI GGNCC 2 cut(s) 295, 330
PsuI RGATCY 1 cut(s) 442
RsaI GTAC 1 cut(s) 158
RsaNI GTAC 1 cut(s) 157
RseI CAYNNNNRTG 1 cut(s) 417
SaqAI TTAA 3 cut(s) 110, 237, 485
SatI GCNGC 2 cut(s) 242, 493
Sau3AI GATC 2 cut(s) 306, 442
Sau96I GGNCC 2 cut(s) 295, 330
SchI GAGTC 1 cut(s) 47
ScrFI CCNGG 1 cut(s) 29
SduI GDGCHC 1 cut(s) 127
SexAI ACCWGGT 1 cut(s) 27
SfaNI GCATC 3 cut(s) 58, 328, 438
SinI GGWCC 1 cut(s) 295
SmiMI CAYNNNNRTG 1 cut(s) 417
Sse9I AATT 2 cut(s) 299, 482
SspI AATATT 1 cut(s) 277
StyD4I CCNGG 1 cut(s) 27
StyI CCWWGG 1 cut(s) 333
TaaI ACNGT 2 cut(s) 188, 291
TasI AATT 2 cut(s) 299, 482
TatI WGTACW 1 cut(s) 156
TfiI GAWTC 1 cut(s) 467
Tru1I TTAA 3 cut(s) 110, 237, 485
Tru9I TTAA 3 cut(s) 110, 237, 485
TscAI CASTG 2 cut(s) 296, 319
TseFI GTSAC 2 cut(s) 9, 82
TseI GCWGC 2 cut(s) 241, 492
Tsp45I GTSAC 2 cut(s) 9, 82
TspDTI ATGAA 2 cut(s) 17, 176
TspRI CASTG 2 cut(s) 296, 319
Van91I CCANNNNNTGG 2 cut(s) 375, 432
VpaK11BI GGWCC 1 cut(s) 295
XcmI CCANNNNNNNNNTGG 1 cut(s) 210
XmnI GAANNNNTTC 1 cut(s) 390
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.