Rh4DG218900

F-box LRR-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0008069
Physical Location & Seq
Forward (+)
41941108 .. 41944850
3743 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG218900.1

Sequence Viewer

Length: 1812 bp
ATGCGACCGATGCGAGGCGGAGGCCGTGATCGGATCAACACGTATCTTCCCGACGAGCTCGTCGTCGAGATCTTCCGGCGCCTTGACTCCAAGCCCAGCCGCGACTCCTGCTCTCTCGTCTGCAAGCGCTGGCTGGGACTCGAGCGGCTGAGCCGCACCACCCTCCGAATCGGCGCCACCGGCAACCCCGACCTCTTCGTCAACCTCCTCTCCCGCCGCTTCGTCAACGTCCGCCACGTCCACATTGACGAGCGCCTCAACATTTCTCTCCCCGTTCACCTCGGGAGCCTGGCGAGGAGGCGCGGGAGTTCTTCGAGTTATAGTAAGAGTGAGGACGATGGGATTGAGACTCATAGCTTATCCGACGCCGGGATGGTTGCTCTCGGTGAAGGCTTTCCGAAGCTCGAAAAGTTGAGCCTTATCTGGTGCTCTAATGTTTCCAGCGTCGGATTGACCTCGCTGGCTGATAAGTGTAGGCTGTTGAAATCATTGGATTTGCAGGGTTGCTATGTGGGAAATCAGGGTGTGGCTGCTGTTGGGCAGTGTTGCAAGCAGCTTGAAGATTTGAATTTGCGTTTTTGTGAGGGATTGACTGACGTTTGTTTGGTAGAGTTAGCCAGTGGTGTTGGGAAGTCATTGAAGTCACTTGGCATTGCAGCTTGTGTAAAGATAACTGATGCTGCATTGGAAGCAGTGGGGTTGCACTGTAAGTCTCTCGAGAGCATGTCGTTGGATGCAGAGTCCATTCACAACAAAGGGGTGCTTGCTGTGGTCCAAGGATGTCCTGCTTTGAAAGTTTTGAAGCTACAATGTATAAATGTCACGGATGAGGTTTTGATAGCTGTGGGAAATTGTTGTTCGTCTTTGGAGTTTTTGGCTCTGTATACCTTCCAGCGGTTTACCGATAAAGGGTTACGTGCTATTGGGAATGGATGTAAGAAGCTAAAAAATCTTACTCTAAGTGACTGCTACTTCCTGAGTGACAAGGCTTTGGAATCAATTGCAATTGGCTGCAAGGAACTTACACATCTTGAAGTGAATGGTTGCCACAATATCGGAACTTTGGGTCTAGAATCCATAGGGAAATCTTGCCCGCGTCTTACTGAATTAGCATTGTTGTACTGCCAGAGGATAGGCAACTTTGCGCTTAGTGAGATTGGACGTGGCTGTAAGTTCTTGCAAGTTCTTCACTTGGTAGATTGCTCAAGTATCGGAGATGAAGCCATTTGCAGTATTGCTAAAGGCTGCAGGAATTTGAAGAAACTTCATATTCGTCGATGTTATGAGATTGGAAACAAGGGAGTTGTAGCAATTGGAGAGCATTGTAGGTCTCTAACCGACCTTAGCCTCCGATTTTGTGACAGAGTGGGGGATGAGGCCCTCATCTCTGTTAGTCAGTGTAGCTCCCTACAATACCTAAATGTCAGTGGCTGCCACCAAATTGGTGATGCTGGATTGATAGCTATTGCAAGAAGTTGTGCAGAACTCACTTACCTAGATGTAAGTGTCCTGCAGAATTTGGGGGATATGTCATTGGCTGAATTAGGTGAAGGCTGTCCAAACCTCAAGGAGATTGTGCTGTCACATTGTCGTCAAATAACAGATGTTGGTTTAAACCATCTTGTCAAAAACTGCACAATGCTTGCATCCTGTCACATGGTTTATTGCCAAGGTATAACTTCTGCTGGAGTAGCCACCGTGGTTTCAAGCTGTCCCAACATAAAGAAAGTCCTGGTTGAGAAGTGGAAGGTTAGCCAGAGGACCAAAAGGAGGGCCGGCTCTATAATTTCTTATCTTTGTGTGGACCTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box-like LRR_6

Protein Analysis

603

Amino Acids

65.38

Weight (kDa)

8.42

Isoelectric Point (pI)

39.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box-like PF12937 16 - 44 6.4e-07 F-box-like
F-box_5 PF18511 16 - 53 2.5e-06 F-box
DUF7885 PF25372 118 - 306 3.5e-13 Leucine Rich Repeat Domain of unknown function (DUF7885)
DUF7885 PF25372 311 - 440 5.5e-13 Leucine Rich Repeat Domain of unknown function (DUF7885)
DUF7885 PF25372 389 - 577 5.8e-22 Leucine Rich Repeat Domain of unknown function (DUF7885)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 59, 197
AccB1I GGYRCC 2 cut(s) 78, 173
AccBSI CCGCTC 1 cut(s) 145
AccI GTMKAC 1 cut(s) 884
AccII CGCG 3 cut(s) 102, 303, 1096
AclWI GGATC 1 cut(s) 41
AcsI RAATTY 3 cut(s) 568, 1252, 1516
AcyI GRCGYC 3 cut(s) 79, 174, 366
AfaI GTAC 1 cut(s) 1121
AfeI AGCGCT 1 cut(s) 128
AfiI CCNNNNNNNGG 5 cut(s) 14, 369, 894, 909, 1770
AflIII ACRYGT 1 cut(s) 39
AgsI TTSAA 9 cut(s) 484, 560, 568, 640, 793, 802, 1034, 1258, 1707
AjiI CACGTC 2 cut(s) 238, 1163
AjnI CCWGG 2 cut(s) 288, 1731
Alw21I GWGCWC 2 cut(s) 60, 431
Alw26I GTCTC 3 cut(s) 341, 717, 1335
AlwI GGATC 1 cut(s) 41
AlwNI CAGNNNCTG 1 cut(s) 1431
Ama87I CYCGRG 3 cut(s) 140, 281, 716
Aor51HI AGCGCT 1 cut(s) 128
AoxI GGCC 3 cut(s) 22, 1377, 1773
ApeKI GCWGC 7 cut(s) 530, 553, 656, 680, 1011, 1245, 1431
ApoI RAATTY 3 cut(s) 568, 1252, 1516
Asp700I GAANNNNTTC 1 cut(s) 393
AspLEI GCGC 6 cut(s) 81, 129, 176, 255, 303, 1147
AspS9I GGNCC 5 cut(s) 772, 1378, 1761, 1773, 1804
AsuC2I CCSGG 1 cut(s) 370
AsuHPI GGTGA 4 cut(s) 269, 398, 1457, 1559
AvaI CYCGRG 3 cut(s) 140, 281, 716
AvaII GGWCC 3 cut(s) 772, 1761, 1804
BanI GGYRCC 2 cut(s) 78, 173
BanII GRGCYC 1 cut(s) 60
Bbv12I GWGCWC 2 cut(s) 60, 431
BbvI GCAGC 7 cut(s) 517, 565, 667, 668, 998, 1232, 1418
BccI CCATC 3 cut(s) 332, 367, 1626
BceAI ACGGC 1 cut(s) 9
BciT130I CCWGG 2 cut(s) 290, 1733
BcnI CCSGG 1 cut(s) 370
BcoDI GTCTC 3 cut(s) 341, 717, 1335
BfaI CTAG 2 cut(s) 1070, 1496
BfmI CTRYAG 2 cut(s) 1246, 1511
BfoI RGCGCY 4 cut(s) 82, 130, 177, 256
BglII AGATCT 1 cut(s) 69
BlpI GCTNAGC 1 cut(s) 149
Bme1390I CCNGG 3 cut(s) 290, 370, 1733
Bme18I GGWCC 3 cut(s) 772, 1761, 1804
BmeT110I CYCGRG 3 cut(s) 140, 281, 716
BmgBI CACGTC 2 cut(s) 238, 1163
BmgT120I GGNCC 5 cut(s) 772, 1378, 1761, 1773, 1804
BmiI GGNNCC 3 cut(s) 80, 175, 287
BmrFI CCNGG 3 cut(s) 290, 370, 1733
BmsI GCATC 4 cut(s) 667, 724, 1438, 1655
BpmI CTGGAG 1 cut(s) 1707
Bpu10I CCTNAGC 1 cut(s) 1343
Bpu1102I GCTNAGC 1 cut(s) 149
BpuEI CTTGAG 2 cut(s) 1189, 1550
BpuMI CCSGG 1 cut(s) 370
BsaAI YACGTR 2 cut(s) 42, 917
BsaHI GRCGYC 3 cut(s) 79, 174, 366
BsaI GGTCTC 1 cut(s) 1335
BsaJI CCNNGG 4 cut(s) 280, 775, 1669, 1698
BsaXI ACNNNNNCTCC 2 cut(s) 194, 224
Bsc4I CCNNNNNNNGG 5 cut(s) 14, 369, 894, 909, 1770
Bse118I RCCGGY 2 cut(s) 179, 1775
Bse1I ACTGG 1 cut(s) 618
Bse3DI GCAATG 1 cut(s) 651
BseBI CCWGG 2 cut(s) 290, 1733
BseDI CCNNGG 4 cut(s) 280, 775, 1669, 1698
BseGI GGATG 7 cut(s) 378, 739, 785, 832, 938, 1378, 1646
BseLI CCNNNNNNNGG 5 cut(s) 14, 369, 894, 909, 1770
BseMI GCAATG 1 cut(s) 651
BseMII CTCAG 2 cut(s) 140, 968
BseNI ACTGG 1 cut(s) 618
BseRI GAGGAG 2 cut(s) 197, 310
BseXI GCAGC 7 cut(s) 517, 565, 667, 668, 998, 1232, 1418
BseYI CCCAGC 2 cut(s) 95, 133
BsgI GTGCAG 2 cut(s) 1500, 1618
Bsh1236I CGCG 3 cut(s) 102, 303, 1096
Bsh1285I CGRYCG 1 cut(s) 8
BshFI GGCC 3 cut(s) 24, 1379, 1775
BshNI GGYRCC 2 cut(s) 78, 173
BsiEI CGRYCG 1 cut(s) 8
BsiHKAI GWGCWC 2 cut(s) 60, 431
BsiHKCI CYCGRG 3 cut(s) 140, 281, 716
BsiSI CCGG 4 cut(s) 76, 180, 369, 1776
BslFI GGGAC 2 cut(s) 150, 1698
BslI CCNNNNNNNGG 5 cut(s) 14, 369, 894, 909, 1770
BsmAI GTCTC 3 cut(s) 341, 717, 1335
BsmFI GGGAC 2 cut(s) 150, 1698
BsnI GGCC 3 cut(s) 24, 1379, 1775
Bso31I GGTCTC 1 cut(s) 1335
BsoBI CYCGRG 3 cut(s) 140, 281, 716
Bsp1286I GDGCHC 2 cut(s) 60, 431
Bsp143I GATC 3 cut(s) 28, 33, 69
Bsp1720I GCTNAGC 1 cut(s) 149
BspANI GGCC 3 cut(s) 24, 1379, 1775
BspCNI CTCAG 2 cut(s) 141, 969
BspFNI CGCG 3 cut(s) 102, 303, 1096
BspLI GGNNCC 3 cut(s) 80, 175, 287
BspMAI CTGCAG 2 cut(s) 1250, 1515
BspPI GGATC 1 cut(s) 41
BspT107I GGYRCC 2 cut(s) 78, 173
BspTNI GGTCTC 1 cut(s) 1335
BsrBI CCGCTC 1 cut(s) 145
BsrDI GCAATG 1 cut(s) 651
BsrFI RCCGGY 2 cut(s) 179, 1775
BsrI ACTGG 1 cut(s) 618
BssAI RCCGGY 2 cut(s) 179, 1775
BssECI CCNNGG 4 cut(s) 280, 775, 1669, 1698
BssMI GATC 3 cut(s) 28, 33, 69
BssNAI GTATAC 1 cut(s) 885
BssNI GRCGYC 3 cut(s) 79, 174, 366
BssT1I CCWWGG 2 cut(s) 775, 1669
Bst1107I GTATAC 1 cut(s) 885
Bst2UI CCWGG 2 cut(s) 290, 1733
Bst4CI ACNGT 2 cut(s) 707, 1699
Bst6I CTCTTC 1 cut(s) 200
BstACI GRCGYC 3 cut(s) 79, 174, 366
BstBAI YACGTR 2 cut(s) 42, 917
BstC8I GCNNGC 8 cut(s) 125, 131, 462, 551, 765, 1094, 1644, 1777
BstDEI CTNAG 5 cut(s) 149, 959, 977, 1148, 1343
BstDSI CCRYGG 1 cut(s) 1698
BstF5I GGATG 7 cut(s) 378, 739, 785, 832, 938, 1378, 1646
BstFNI CGCG 3 cut(s) 102, 303, 1096
BstH2I RGCGCY 4 cut(s) 82, 130, 177, 256
BstHHI GCGC 6 cut(s) 81, 129, 176, 255, 303, 1147
BstKTI GATC 3 cut(s) 31, 36, 72
BstMAI GTCTC 3 cut(s) 341, 717, 1335
BstMBI GATC 3 cut(s) 28, 33, 69
BstMCI CGRYCG 1 cut(s) 8
BstMWI GCNNNNNNNGC 5 cut(s) 10, 108, 180, 689, 1691
BstNI CCWGG 2 cut(s) 290, 1733
BstNSI RCATGY 1 cut(s) 727
BstSCI CCNGG 3 cut(s) 288, 368, 1731
BstSFI CTRYAG 2 cut(s) 1246, 1511
BstUI CGCG 3 cut(s) 102, 303, 1096
BstV1I GCAGC 7 cut(s) 517, 565, 667, 668, 998, 1232, 1418
BstX2I RGATCY 1 cut(s) 69
BstXI CCANNNNNNTGG 1 cut(s) 1442
BstYI RGATCY 1 cut(s) 69
BstZ17I GTATAC 1 cut(s) 885
BsuRI GGCC 3 cut(s) 24, 1379, 1775
BtgI CCRYGG 1 cut(s) 1698
BtrI CACGTC 2 cut(s) 238, 1163
BtsCI GGATG 7 cut(s) 378, 739, 785, 832, 938, 1378, 1646
BtsI GCAGTG 2 cut(s) 548, 699
BtsIMutI CAGTG 6 cut(s) 548, 625, 699, 703, 1403, 1432
Cac8I GCNNGC 8 cut(s) 125, 131, 462, 551, 765, 1094, 1644, 1777
CaiI CAGNNNCTG 1 cut(s) 1431
CfoI GCGC 6 cut(s) 81, 129, 176, 255, 303, 1147
Cfr10I RCCGGY 2 cut(s) 179, 1775
Cfr13I GGNCC 5 cut(s) 772, 1378, 1761, 1773, 1804
CseI GACGC 3 cut(s) 374, 433, 1085
Csp6I GTAC 1 cut(s) 1120
CviAII CATG 2 cut(s) 724, 1657
CviQI GTAC 1 cut(s) 1120
DdeI CTNAG 5 cut(s) 149, 959, 977, 1148, 1343
DinI GGCGCC 2 cut(s) 80, 175
DpnI GATC 3 cut(s) 30, 35, 71
DpnII GATC 3 cut(s) 28, 33, 69
DraI TTTAAA 1 cut(s) 1614
DrdI GACNNNNNNGTC 2 cut(s) 59, 197
DseDI GACNNNNNNGTC 2 cut(s) 59, 197
Eam1104I CTCTTC 1 cut(s) 200
EarI CTCTTC 1 cut(s) 200
EciI GGCGGA 2 cut(s) 33, 221
Ecl136II GAGCTC 1 cut(s) 58
Eco130I CCWWGG 2 cut(s) 775, 1669
Eco24I GRGCYC 1 cut(s) 60
Eco31I GGTCTC 1 cut(s) 1335
Eco47I GGWCC 3 cut(s) 772, 1761, 1804
Eco47III AGCGCT 1 cut(s) 128
Eco53kI GAGCTC 1 cut(s) 58
Eco88I CYCGRG 3 cut(s) 140, 281, 716
EcoICRI GAGCTC 1 cut(s) 58
EcoO109I RGGNCCY 1 cut(s) 1378
EcoRII CCWGG 2 cut(s) 288, 1731
EcoT14I CCWWGG 2 cut(s) 775, 1669
EcoT38I GRGCYC 1 cut(s) 60
EgeI GGCGCC 2 cut(s) 80, 175
EheI GGCGCC 2 cut(s) 80, 175
ErhI CCWWGG 2 cut(s) 775, 1669
FaeI CATG 2 cut(s) 727, 1660
FalI AAGNNNNNCTT 2 cut(s) 747, 779
FaqI GGGAC 2 cut(s) 150, 1698
FatI CATG 2 cut(s) 723, 1656
FauI CCCGC 3 cut(s) 221, 296, 1101
FblI GTMKAC 1 cut(s) 884
FokI GGATG 7 cut(s) 385, 746, 792, 839, 945, 1385, 1633
FriOI GRGCYC 1 cut(s) 60
FspBI CTAG 2 cut(s) 1070, 1496
GlaI GCGC 6 cut(s) 80, 128, 175, 254, 302, 1146
GsaI CCCAGC 2 cut(s) 99, 137
GsuI CTGGAG 1 cut(s) 1707
HaeII RGCGCY 4 cut(s) 82, 130, 177, 256
HaeIII GGCC 3 cut(s) 24, 1379, 1775
HapII CCGG 4 cut(s) 76, 180, 369, 1776
HgaI GACGC 3 cut(s) 374, 433, 1085
HhaI GCGC 6 cut(s) 81, 129, 176, 255, 303, 1147
Hin1I GRCGYC 3 cut(s) 79, 174, 366
Hin1II CATG 2 cut(s) 727, 1660
Hin6I GCGC 6 cut(s) 79, 127, 174, 253, 301, 1145
HinP1I GCGC 6 cut(s) 79, 127, 174, 253, 301, 1145
HincII GTYRAC 2 cut(s) 202, 226
HindII GTYRAC 2 cut(s) 202, 226
HinfI GANTC 8 cut(s) 86, 104, 138, 168, 349, 740, 995, 1073
HpaII CCGG 4 cut(s) 76, 180, 369, 1776
HphI GGTGA 4 cut(s) 269, 398, 1457, 1559
Hpy166II GTNNAC 7 cut(s) 202, 226, 241, 277, 885, 900, 1804
Hpy188I TCNGA 8 cut(s) 33, 167, 364, 399, 449, 1058, 1214, 1352
Hpy188III TCNNGA 8 cut(s) 50, 67, 283, 716, 718, 976, 1031, 1070
Hpy8I GTNNAC 7 cut(s) 202, 226, 241, 277, 885, 900, 1804
Hpy99I CGWCG 6 cut(s) 56, 65, 68, 368, 449, 1278
HpyAV CCTTC 4 cut(s) 383, 898, 1544, 1741
HpyCH4III ACNGT 2 cut(s) 707, 1699
HpyCH4IV ACGT 6 cut(s) 41, 228, 237, 597, 916, 1162
HpyF10VI GCNNNNNNNGC 5 cut(s) 10, 108, 180, 689, 1691
HpyF3I CTNAG 5 cut(s) 149, 959, 977, 1148, 1343
HpySE526I ACGT 6 cut(s) 41, 228, 237, 597, 916, 1162
Hsp92I GRCGYC 3 cut(s) 79, 174, 366
Hsp92II CATG 2 cut(s) 727, 1660
HspAI GCGC 6 cut(s) 79, 127, 174, 253, 301, 1145
KasI GGCGCC 2 cut(s) 78, 173
KroI GCCGGC 1 cut(s) 1775
KroNI GCCGGC 1 cut(s) 1777
Kzo9I GATC 3 cut(s) 28, 33, 69
LmnI GCTCC 2 cut(s) 285, 1409
Lsp1109I GCAGC 7 cut(s) 517, 565, 667, 668, 998, 1232, 1418
LweI GCATC 4 cut(s) 667, 724, 1438, 1655
MaeI CTAG 2 cut(s) 1070, 1496
MaeII ACGT 6 cut(s) 41, 228, 237, 597, 916, 1162
MaeIII GTNAC 8 cut(s) 642, 820, 912, 962, 980, 1358, 1581, 1652
MalI GATC 3 cut(s) 30, 35, 71
MbiI CCGCTC 1 cut(s) 145
MboI GATC 3 cut(s) 28, 33, 69
MboII GAAGA 7 cut(s) 38, 64, 187, 303, 572, 1178, 1270
MfeI CAATTG 3 cut(s) 999, 1005, 1311
MflI RGATCY 1 cut(s) 69
MhlI GDGCHC 2 cut(s) 60, 431
Mly113I GGCGCC 2 cut(s) 79, 174
MlyI GAGTC 5 cut(s) 80, 98, 132, 343, 749
MmeI TCCRAC 3 cut(s) 387, 427, 711
MroNI GCCGGC 1 cut(s) 1775
MroXI GAANNNNTTC 1 cut(s) 393
MseI TTAA 1 cut(s) 1613
MspA1I CMGCKG 1 cut(s) 895
MspI CCGG 4 cut(s) 76, 180, 369, 1776
MspR9I CCNGG 3 cut(s) 290, 370, 1733
MssI GTTTAAAC 1 cut(s) 1614
MunI CAATTG 3 cut(s) 999, 1005, 1311
MvaI CCWGG 2 cut(s) 290, 1733
MvnI CGCG 3 cut(s) 102, 303, 1096
MwoI GCNNNNNNNGC 5 cut(s) 10, 108, 180, 689, 1691
NaeI GCCGGC 1 cut(s) 1777
NarI GGCGCC 2 cut(s) 79, 174
NciI CCSGG 1 cut(s) 370
NdeII GATC 3 cut(s) 28, 33, 69
NgoMIV GCCGGC 1 cut(s) 1775
NlaIII CATG 2 cut(s) 727, 1660
NlaIV GGNNCC 3 cut(s) 80, 175, 287
NmuCI GTSAC 7 cut(s) 642, 820, 962, 980, 1358, 1581, 1652
NspI RCATGY 1 cut(s) 727
PaeR7I CTCGAG 2 cut(s) 140, 716
PcsI WCGNNNNNNNCGW 2 cut(s) 60, 234
PdiI GCCGGC 1 cut(s) 1777
PdmI GAANNNNTTC 1 cut(s) 393
PfeI GAWTC 3 cut(s) 168, 995, 1073
PleI GAGTC 5 cut(s) 80, 98, 132, 343, 748
PluTI GGCGCC 2 cut(s) 82, 177
PmeI GTTTAAAC 1 cut(s) 1614
PpsI GAGTC 5 cut(s) 80, 98, 132, 343, 748
Ppu21I YACGTR 2 cut(s) 42, 917
Psp124BI GAGCTC 1 cut(s) 60
Psp6I CCWGG 2 cut(s) 288, 1731
PspFI CCCAGC 2 cut(s) 95, 133
PspGI CCWGG 2 cut(s) 288, 1731
PspN4I GGNNCC 3 cut(s) 80, 175, 287
PspPI GGNCC 5 cut(s) 772, 1378, 1761, 1773, 1804
PspXI VCTCGAGB 1 cut(s) 140
PstI CTGCAG 2 cut(s) 1250, 1515
PstNI CAGNNNCTG 1 cut(s) 1431
PsuI RGATCY 1 cut(s) 69
RsaI GTAC 1 cut(s) 1121
RsaNI GTAC 1 cut(s) 1120
SacI GAGCTC 1 cut(s) 60
SaqAI TTAA 1 cut(s) 1613
Sau3AI GATC 3 cut(s) 28, 33, 69
Sau96I GGNCC 5 cut(s) 772, 1378, 1761, 1773, 1804
SchI GAGTC 5 cut(s) 80, 98, 132, 343, 749
ScrFI CCNGG 3 cut(s) 290, 370, 1733
SduI GDGCHC 2 cut(s) 60, 431
SfaNI GCATC 4 cut(s) 667, 724, 1438, 1655
SfcI CTRYAG 2 cut(s) 1246, 1511
SfoI GGCGCC 2 cut(s) 80, 175
Sfr274I CTCGAG 2 cut(s) 140, 716
SinI GGWCC 3 cut(s) 772, 1761, 1804
SlaI CTCGAG 2 cut(s) 140, 716
SmlI CTYRAG 4 cut(s) 140, 716, 1204, 1565
SmoI CTYRAG 4 cut(s) 140, 716, 1204, 1565
SspDI GGCGCC 2 cut(s) 78, 173
SspMI CTAG 2 cut(s) 1070, 1496
SstI GAGCTC 1 cut(s) 60
StyD4I CCNGG 3 cut(s) 288, 368, 1731
StyI CCWWGG 2 cut(s) 775, 1669
TaaI ACNGT 2 cut(s) 707, 1699
TaiI ACGT 6 cut(s) 44, 231, 240, 600, 919, 1165
TaqI TCGA 6 cut(s) 66, 141, 314, 405, 717, 1276
TaqII GACCGA 1 cut(s) 22
TatI WGTACW 1 cut(s) 1119
TauI GCSGC 4 cut(s) 102, 148, 156, 219
TfiI GAWTC 3 cut(s) 168, 995, 1073
Tru1I TTAA 1 cut(s) 1613
Tru9I TTAA 1 cut(s) 1613
TscAI CASTG 6 cut(s) 548, 625, 699, 710, 1403, 1432
TseFI GTSAC 7 cut(s) 642, 820, 962, 980, 1358, 1581, 1652
TseI GCWGC 7 cut(s) 530, 553, 656, 680, 1011, 1245, 1431
Tsp45I GTSAC 7 cut(s) 642, 820, 962, 980, 1358, 1581, 1652
TspDTI ATGAA 2 cut(s) 1233, 1256
TspGWI ACGGA 1 cut(s) 839
TspRI CASTG 6 cut(s) 548, 625, 699, 710, 1403, 1432
VpaK11BI GGWCC 3 cut(s) 772, 1761, 1804
XapI RAATTY 3 cut(s) 568, 1252, 1516
XbaI TCTAGA 1 cut(s) 1069
XceI RCATGY 1 cut(s) 727
XhoI CTCGAG 2 cut(s) 140, 716
XmiI GTMKAC 1 cut(s) 884
XmnI GAANNNNTTC 1 cut(s) 393
XspI CTAG 2 cut(s) 1070, 1496
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.