Rorug04G0178900
ERF Family

Belongs to the class I-like SAM-binding methyltransferase superfamily. Protein arginine N- methyltransferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0003804
Physical Location & Seq
Forward (+)
31413474 .. 31427994
14521 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0178900.1

Sequence Viewer

Length: 2646 bp
ATGCAAGGCAAGCACTCTGTTTATTGGCCATTGGATGCTAATTCATATTCCGGTCGCGTCGTGGGATACAACTCTGACAGTACTAAGCATCATGTTGAATATGAAGATGGTGATGAGGAAGATTTGATTCTTTCAAACGAGAGAGTTAAGTTTTGTATTTCTCGGGATGAAATGGAGAGTTTGAATTTGAGTGCCAGTCTCAAGAATCTGGATAGTGATGTGTATGATTATAATGAGATGGTTGTGCTAGCAGCGAGTTTGGATGACTGTCAGGAACTAGAGCCTGGGGATATCATCTGGGCCAAGCTTACTGGCTATGCTATGTGGCCAGCAATTGTTGTGGATGAATCACTTATTGGTGACCGTGAAGGCTTAAGTAAAACTTTGGTAGGAGGATCAGTTCCAGTGCAATTCTTTGGTACCCATGACTTTGCAAGGATTAAAGTGAAGCAGGCTATCTCATTTCTCAAAGGACTTCTTTCTTCATTCCATTTGAAGTGCAGGAAGTCTCGATTTATTAAAAGCTTGGAAGAAGCGAAAATGTATCTCAGTGAACAAAAGCTTCCTAGAAGAATGCTACGATTGCAAAAAGGAATAAACGTTGATGATTGTGAAAGTGAAAATGGTGAGGATGGAATGCGCACTGATTCAGGAGAAGGATGTCTGGATGATGTCATGATTCCAAGGTCGCTGGATTGCCTTGGAACTTCTGTGTTAGTGATGGGGGATCTGCAGATAATAAGACTAGGAAGGATTGTGAGGGAATCAAAATATTTTCAGGATGAGAGATACGTTTGGCCTGAAGGTTATACTGCAGTAAGGAAGTTTACTTCAATAACAGATCCGAGTGCATGTACCATGTATAAGATGGAGGTACTGAGAGATCCTGAATCAAAAATTCGACCTCTGTTCCAAGTCTCAATAGACACTGGAGAGCAGTTCAAGGGATCGACACCGTCGGCTTGCTGGGATAAAATATACAAAAGGATAAAGAAAATACAAAACAGTGCTCTTGATGATTCTGATGCTAATGCTGATGATGGATTAGAAAAGCTCTATAAATCTGGCTCTGAGATGTTTGGTTTCTCTAAACCTGAAGTTGCAAAACTTATACAGGGGTTATTAAAATCCAGCCATGCTTCAAAATTAGCCAAGTGCAAGTCAACCTCTAGAAGATATCGAGATGTTCCTGTTGGGTACAGACCAGTTCGTGTGGACTGGAAAGACCTTGATAAATGCAGTGTTTGCCACATGGATGAGGAGTACGAAAATAACCTGTTCCTGCAGTGTGACAAATGCAGAATGATGGTCCATGCAAGATGCTATGGAGAATTGGAACCTGTTGGTGGTGTTCTATGGTTATGCAACTTATGCCGGCCTGGAGCTCCTGAACCTCCACCACCTTGCTGCCTTTGTCCTGTAATAGGGGGTGCAATGAAGCCTACAACTGATGGGCGCTGGGCTCATTTGGCTTGCGCCATATGGATACCAGAAACTTGCCTATCTGATATCAAGAGAATGGAACCTATTGATGGGTTGAGCAGAATCAATAAGGATCGTTGGAAGCTGTTATGTAGTATCTGTGGTGTTTCTTATGGAGCCTGTATTCAATGTTCAAACCATACTTGTTATGCGGCATATCATCCACTTTGTGCACGAGCTGCTGGTCTTTGTGTTGAGCTCGAGGACGAAGAGAGACTGCATCTACTTTCAGTAGATGATGATGAAGAAGGCCAATGCATTCGCTTTCTTTCCTTTTGCAAGAGGCATAAGCAGCCATCTAATGACCGTTCAGCGGCTGGTGACCGGATTGGCCGAACTGTGCGCCAATGTTCAGACTACACCCCACCATCTAATCCATCTGGATGTGCTCGTACTGAGCCATACAATTACTCCTGTAGAAGAGGGAGAAAAGAACCTGAAGCAATAGCTGCTGCATCCTTAAAGCGTTTGTTTGTTGAGAACCAACCTTACTTAGTTGGTGGGTACAGCCAACACCAATTGTCAAGCAATCCACTGCCTCCCAATTGTGTTGTTGGCTCTAAGTTCAGTTCTAGGCTTCTGAGGCTGAAAGCTTCTCAACTTGATGCTCCTACCGATATACTTTCTATGGCTGAGAAGTATAAGTACATGAGGGATACTTTTCGAAAAAGACTAGCGTTTGGAAAATCGGGAATTCATGGATTTGGTATATTTGCGAAGCACCCACATAGAGCAGGGGACATGGTGATTGAATACACTGGTGAACTTGTCAGACCTCCTATAGCTGACAGGAGAGAGCGCTTCATATATAATTCATTGGTGGGGGCTGGGACTTATATGTTCCGGATTGATGATGAACGAGTCATTGATGCTACACGGGCTGGAAGCATTGCTCACCTGATCAATCACTCTTGTGAACCAAATTGCTACTCAAGAGTCATAAGTGTGAATTCTGACGAGCATATAATTATATTTGCAAAGAGAGACATTAAAAGATGGGAAGAACTTACATATGATTATAGATTTTTTTCAATCGATGAACAACTTGCATGTTATTGTGGTTTTCCAAGATGCCGGGGGGTTGTTAATGATGTTGACTCAGAAGAGCGAGGGACAAAGCTTTATGCGTCTCGCAGTGAATTAATAGATTGGACAGGAGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0003006 GO:0003674 GO:0003824 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006325 GO:0006355 GO:0006464 GO:0006479 GO:0006807 GO:0006950 GO:0006970 GO:0006996 GO:0007275 GO:0008150 GO:0008152 GO:0008168 GO:0008170 GO:0008213 GO:0008276 GO:0008469 GO:0008757 GO:0009628 GO:0009651 GO:0009791 GO:0009889 GO:0009891 GO:0009893 GO:0009909 GO:0009987 GO:0010035 GO:0010038 GO:0010228 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0016043 GO:0016273 GO:0016274 GO:0016277 GO:0016569 GO:0016570 GO:0016571 GO:0016740 GO:0016741 GO:0018193 GO:0018195 GO:0018216 GO:0019219 GO:0019222 GO:0019538 GO:0019919 GO:0022414 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0032259 GO:0032501 GO:0032502 GO:0034969 GO:0034970 GO:0034971 GO:0034972 GO:0035241 GO:0035242 GO:0035246 GO:0035247 GO:0036211 GO:0042054 GO:0042221 GO:0042802 GO:0042803 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043414 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0045893 GO:0045935 GO:0046686 GO:0046982 GO:0046983 GO:0048518 GO:0048522 GO:0048580 GO:0048583 GO:0048584 GO:0048608 GO:0048731 GO:0048831 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051239 GO:0051252 GO:0051254 GO:0051276 GO:0060255 GO:0061458 GO:0065007 GO:0071704 GO:0071840 GO:0080090 GO:0080134 GO:0140096 GO:1901564 GO:1902680 GO:1902882 GO:1902884 GO:1903506 GO:1903508 GO:2000026 GO:2000112 GO:2000241 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko01522 map01522
Pfam Domains
Protein Families
Methyltransf_25 PrmA

Protein Analysis

881

Amino Acids

99.63

Weight (kDa)

6.41

Isoelectric Point (pI)

40.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PTM_DIR17_Tudor PF21743 14 - 43 3.5e-07 PTM/DIR17-like, Tudor domain
PWWP PF00855 96 - 183 8.9e-16 PWWP domain
FYRN PF05964 239 - 294 2.9e-17 F/Y-rich N-terminus
FYRC PF05965 301 - 374 1.1e-11 F/Y rich C-terminus
PHD_2 PF13831 425 - 458 1.9e-06 PHD-finger
zf-HC5HC2H_2 PF13832 468 - 589 1.6e-32 PHD-zinc-finger like domain
zf-HC5HC2H PF13771 489 - 591 1.3e-19 PHD-like zinc-binding domain
SET PF00856 728 - 834 5.8e-25 SET domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 231
Acc16I TGCGCA 1 cut(s) 641
Acc65I GGTACC 1 cut(s) 419
AccB1I GGYRCC 1 cut(s) 419
AccII CGCG 1 cut(s) 57
AccIII TCCGGA 1 cut(s) 2325
AciI CCGC 2 cut(s) 1634, 1796
AclI AACGTT 1 cut(s) 600
AclWI GGATC 6 cut(s) 403, 735, 836, 878, 955, 1563
AcoI YGGCCR 3 cut(s) 26, 326, 1813
AcsI RAATTY 4 cut(s) 184, 897, 2175, 2431
AcuI CTGAAG 3 cut(s) 822, 1116, 1941
AdeI CACNNNGTG 1 cut(s) 1652
AfaI GTAC 9 cut(s) 82, 421, 856, 876, 1199, 1265, 1876, 1988, 2129
AfeI AGCGCT 1 cut(s) 2282
AfiI CCNNNNNNNGG 4 cut(s) 1346, 1424, 1532, 1795
AflII CTTAAG 1 cut(s) 373
AjnI CCWGG 2 cut(s) 283, 1378
AjuI GAANNNNNNNTTGG 2 cut(s) 339, 371
AleI CACNNNNGTG 1 cut(s) 2394
AloI GAACNNNNNNTCC 2 cut(s) 384, 416
Alw21I GWGCWC 5 cut(s) 1012, 1387, 1657, 1683, 1873
Alw26I GTCTC 6 cut(s) 203, 513, 922, 1690, 2460, 2616
Alw44I GTGCAC 1 cut(s) 1653
AlwI GGATC 6 cut(s) 403, 735, 836, 878, 955, 1563
AlwNI CAGNNNCTG 1 cut(s) 1799
Ama87I CYCGRG 2 cut(s) 162, 1682
Aor13HI TCCGGA 1 cut(s) 2325
Aor51HI AGCGCT 1 cut(s) 2282
AoxI GGCC 7 cut(s) 26, 300, 326, 797, 1376, 1732, 1813
ApaLI GTGCAC 1 cut(s) 1653
ApeKI GCWGC 6 cut(s) 251, 1407, 1661, 1774, 1931, 1934
ApoI RAATTY 4 cut(s) 184, 897, 2175, 2431
AseI ATTAAT 1 cut(s) 2624
Asp718I GGTACC 1 cut(s) 419
AspLEI GCGC 5 cut(s) 642, 1458, 1478, 1827, 2283
AspS9I GGNCC 2 cut(s) 300, 1309
AsuC2I CCSGG 1 cut(s) 2558
AsuHPI GGTGA 7 cut(s) 122, 371, 638, 1814, 2239, 2255, 2369
AsuII TTCGAA 1 cut(s) 2146
AsuNHI GCTAGC 1 cut(s) 247
AvaI CYCGRG 2 cut(s) 162, 1682
AvaII GGWCC 1 cut(s) 1309
BaeGI GKGCMC 1 cut(s) 1657
BalI TGGCCA 2 cut(s) 28, 328
BanI GGYRCC 1 cut(s) 419
BanII GRGCYC 3 cut(s) 1387, 1465, 1683
BarI GAAGNNNNNNTAC 2 cut(s) 2111, 2143
BauI CACGAG 1 cut(s) 1656
Bbv12I GWGCWC 5 cut(s) 1012, 1387, 1657, 1683, 1873
BbvI GCAGC 6 cut(s) 263, 1394, 1648, 1786, 1918, 1921
BciT130I CCWGG 2 cut(s) 285, 1380
BciVI GTATCC 3 cut(s) 59, 1479, 2131
BclI TGATCA 1 cut(s) 2382
BcnI CCSGG 1 cut(s) 2558
BcoDI GTCTC 6 cut(s) 203, 513, 922, 1690, 2460, 2616
BfaI CTAG 7 cut(s) 248, 278, 567, 746, 1170, 2055, 2156
BfmI CTRYAG 5 cut(s) 731, 813, 1283, 1897, 2262
BfoI RGCGCY 2 cut(s) 1459, 2284
BfrI CTTAAG 1 cut(s) 373
BfuI GTATCC 3 cut(s) 59, 1479, 2131
BisI GCNGC 8 cut(s) 252, 1408, 1635, 1662, 1775, 1797, 1932, 1935
BlsI GCNGC 8 cut(s) 253, 1409, 1636, 1663, 1776, 1798, 1933, 1936
BmcAI AGTACT 1 cut(s) 82
Bme1390I CCNGG 3 cut(s) 285, 1380, 2558
Bme18I GGWCC 1 cut(s) 1309
BmeT110I CYCGRG 2 cut(s) 162, 1682
BmgT120I GGNCC 2 cut(s) 300, 1309
BmiI GGNNCC 4 cut(s) 421, 1338, 1524, 1600
BmrFI CCNGG 3 cut(s) 285, 1380, 2558
BmsI GCATC 9 cut(s) 25, 97, 1015, 1310, 1711, 1946, 2077, 2341, 2543
BmtI GCTAGC 1 cut(s) 251
BpmI CTGGAG 2 cut(s) 951, 1401
Bpu14I TTCGAA 1 cut(s) 2146
BpuEI CTTGAG 2 cut(s) 185, 2398
BpuMI CCSGG 1 cut(s) 2558
Bsa29I ATCGAT 1 cut(s) 2517
BsaJI CCNNGG 4 cut(s) 284, 683, 700, 2557
BsaWI WCCGGW 3 cut(s) 50, 1806, 2325
BsaXI ACNNNNNCTCC 4 cut(s) 384, 414, 1877, 1907
Bsc4I CCNNNNNNNGG 4 cut(s) 1346, 1424, 1532, 1795
Bse118I RCCGGY 1 cut(s) 1374
Bse1I ACTGG 7 cut(s) 195, 316, 404, 934, 1205, 1223, 2245
Bse3DI GCAATG 2 cut(s) 1440, 2370
BseAI TCCGGA 1 cut(s) 2325
BseBI CCWGG 2 cut(s) 285, 1380
BseCI ATCGAT 1 cut(s) 2517
BseDI CCNNGG 4 cut(s) 284, 683, 700, 2557
BseLI CCNNNNNNNGG 4 cut(s) 1346, 1424, 1532, 1795
BseMI GCAATG 2 cut(s) 1440, 2370
BseMII CTCAG 7 cut(s) 562, 869, 1062, 1869, 2054, 2106, 2595
BseNI ACTGG 7 cut(s) 195, 316, 404, 934, 1205, 1223, 2245
BseRI GAGGAG 1 cut(s) 1274
BseSI GKGCMC 1 cut(s) 1657
BseXI GCAGC 6 cut(s) 263, 1394, 1648, 1786, 1918, 1921
BseYI CCCAGC 3 cut(s) 966, 1458, 2309
BsgI GTGCAG 1 cut(s) 520
Bsh1236I CGCG 1 cut(s) 57
Bsh1285I CGRYCG 1 cut(s) 55
BshFI GGCC 7 cut(s) 28, 302, 328, 799, 1378, 1734, 1815
BshNI GGYRCC 1 cut(s) 419
BshVI ATCGAT 1 cut(s) 2517
BsiEI CGRYCG 1 cut(s) 55
BsiHKAI GWGCWC 5 cut(s) 1012, 1387, 1657, 1683, 1873
BsiHKCI CYCGRG 2 cut(s) 162, 1682
BsiSI CCGG 5 cut(s) 51, 1375, 1807, 2326, 2557
BslFI GGGAC 3 cut(s) 2234, 2326, 2608
BslI CCNNNNNNNGG 4 cut(s) 1346, 1424, 1532, 1795
BsmAI GTCTC 6 cut(s) 203, 513, 922, 1690, 2460, 2616
BsmBI CGTCTC 1 cut(s) 2616
BsmFI GGGAC 3 cut(s) 2234, 2326, 2608
BsmI GAATGC 3 cut(s) 579, 642, 1740
BsnI GGCC 7 cut(s) 28, 302, 328, 799, 1378, 1734, 1815
BsoBI CYCGRG 2 cut(s) 162, 1682
Bsp119I TTCGAA 1 cut(s) 2146
Bsp1286I GDGCHC 6 cut(s) 1012, 1387, 1465, 1657, 1683, 1873
Bsp13I TCCGGA 1 cut(s) 2325
Bsp143I GATC 7 cut(s) 395, 727, 841, 883, 947, 1555, 2382
BspACI CCGC 2 cut(s) 1634, 1796
BspANI GGCC 7 cut(s) 28, 302, 328, 799, 1378, 1734, 1815
BspCNI CTCAG 7 cut(s) 561, 870, 1063, 1870, 2055, 2107, 2594
BspDI ATCGAT 1 cut(s) 2517
BspEI TCCGGA 1 cut(s) 2325
BspFNI CGCG 1 cut(s) 57
BspHI TCATGA 1 cut(s) 675
BspLI GGNNCC 4 cut(s) 421, 1338, 1524, 1600
BspMAI CTGCAG 3 cut(s) 735, 817, 1287
BspOI GCTAGC 1 cut(s) 251
BspPI GGATC 6 cut(s) 403, 735, 836, 878, 955, 1563
BspQI GCTCTTC 1 cut(s) 2580
BspT104I TTCGAA 1 cut(s) 2146
BspT107I GGYRCC 1 cut(s) 419
BspTI CTTAAG 1 cut(s) 373
BsrDI GCAATG 2 cut(s) 1440, 2370
BsrFI RCCGGY 1 cut(s) 1374
BsrI ACTGG 7 cut(s) 195, 316, 404, 934, 1205, 1223, 2245
BssAI RCCGGY 1 cut(s) 1374
BssECI CCNNGG 4 cut(s) 284, 683, 700, 2557
BssMI GATC 7 cut(s) 395, 727, 841, 883, 947, 1555, 2382
BssSI CACGAG 1 cut(s) 1656
BssT1I CCWWGG 2 cut(s) 683, 700
Bst2BI CACGAG 1 cut(s) 1656
Bst2UI CCWGG 2 cut(s) 285, 1380
Bst4CI ACNGT 7 cut(s) 80, 269, 365, 957, 1007, 1790, 1822
Bst6I CTCTTC 3 cut(s) 1686, 1897, 2580
BstAFI CTTAAG 1 cut(s) 373
BstAPI GCANNNNNTGC 4 cut(s) 1245, 1371, 1661, 1931
BstBI TTCGAA 1 cut(s) 2146
BstC8I GCNNGC 7 cut(s) 11, 249, 330, 453, 964, 1376, 1474
BstEII GGTNACC 2 cut(s) 359, 1802
BstENI CCTNNNNNAGG 1 cut(s) 1422
BstFNI CGCG 1 cut(s) 57
BstH2I RGCGCY 2 cut(s) 1459, 2284
BstHHI GCGC 5 cut(s) 642, 1458, 1478, 1827, 2283
BstKTI GATC 7 cut(s) 398, 730, 844, 886, 950, 1558, 2385
BstMAI GTCTC 6 cut(s) 203, 513, 922, 1690, 2460, 2616
BstMBI GATC 7 cut(s) 395, 727, 841, 883, 947, 1555, 2382
BstMCI CGRYCG 1 cut(s) 55
BstNI CCWGG 2 cut(s) 285, 1380
BstNSI RCATGY 2 cut(s) 855, 2535
BstPI GGTNACC 2 cut(s) 359, 1802
BstSCI CCNGG 3 cut(s) 283, 1378, 2556
BstSFI CTRYAG 5 cut(s) 731, 813, 1283, 1897, 2262
BstSLI GKGCMC 1 cut(s) 1657
BstUI CGCG 1 cut(s) 57
BstV1I GCAGC 6 cut(s) 263, 1394, 1648, 1786, 1918, 1921
BstX2I RGATCY 3 cut(s) 727, 841, 883
BstYI RGATCY 3 cut(s) 727, 841, 883
Bsu15I ATCGAT 1 cut(s) 2517
BsuI GTATCC 3 cut(s) 59, 1479, 2131
BsuRI GGCC 7 cut(s) 28, 302, 328, 799, 1378, 1734, 1815
BsuTUI ATCGAT 1 cut(s) 2517
BtsI GCAGTG 4 cut(s) 1246, 1292, 2015, 2623
Cac8I GCNNGC 7 cut(s) 11, 249, 330, 453, 964, 1376, 1474
CaiI CAGNNNCTG 1 cut(s) 1799
CciI TCATGA 1 cut(s) 675
CfoI GCGC 5 cut(s) 642, 1458, 1478, 1827, 2283
Cfr10I RCCGGY 1 cut(s) 1374
Cfr13I GGNCC 2 cut(s) 300, 1309
ClaI ATCGAT 1 cut(s) 2517
CseI GACGC 2 cut(s) 46, 2598
Csp6I GTAC 9 cut(s) 81, 420, 855, 875, 1198, 1264, 1875, 1987, 2128
CspCI CAANNNNNGTGG 2 cut(s) 321, 356
CviQI GTAC 9 cut(s) 81, 420, 855, 875, 1198, 1264, 1875, 1987, 2128
DpnI GATC 7 cut(s) 397, 729, 843, 885, 949, 1557, 2384
DpnII GATC 7 cut(s) 395, 727, 841, 883, 947, 1555, 2382
DraIII CACNNNGTG 1 cut(s) 1652
EaeI YGGCCR 3 cut(s) 26, 326, 1813
Eam1104I CTCTTC 3 cut(s) 1686, 1897, 2580
EarI CTCTTC 3 cut(s) 1686, 1897, 2580
Ecl136II GAGCTC 2 cut(s) 1385, 1681
Eco130I CCWWGG 2 cut(s) 683, 700
Eco24I GRGCYC 3 cut(s) 1387, 1465, 1683
Eco32I GATATC 3 cut(s) 292, 1178, 1510
Eco47I GGWCC 1 cut(s) 1309
Eco47III AGCGCT 1 cut(s) 2282
Eco53kI GAGCTC 2 cut(s) 1385, 1681
Eco57I CTGAAG 3 cut(s) 822, 1116, 1941
Eco88I CYCGRG 2 cut(s) 162, 1682
Eco91I GGTNACC 2 cut(s) 359, 1802
EcoICRI GAGCTC 2 cut(s) 1385, 1681
EcoNI CCTNNNNNAGG 1 cut(s) 1422
EcoO65I GGTNACC 2 cut(s) 359, 1802
EcoRI GAATTC 2 cut(s) 2175, 2431
EcoRII CCWGG 2 cut(s) 283, 1378
EcoRV GATATC 3 cut(s) 292, 1178, 1510
EcoT14I CCWWGG 2 cut(s) 683, 700
EcoT22I ATGCAT 1 cut(s) 1742
EcoT38I GRGCYC 3 cut(s) 1387, 1465, 1683
ErhI CCWWGG 2 cut(s) 683, 700
Esp3I CGTCTC 1 cut(s) 2616
FalI AAGNNNNNCTT 4 cut(s) 367, 399, 462, 494
FaqI GGGAC 3 cut(s) 2234, 2326, 2608
FauNDI CATATG 2 cut(s) 1481, 2494
FbaI TGATCA 1 cut(s) 2382
Fnu4HI GCNGC 8 cut(s) 252, 1408, 1635, 1662, 1775, 1797, 1932, 1935
FriOI GRGCYC 3 cut(s) 1387, 1465, 1683
Fsp4HI GCNGC 8 cut(s) 252, 1408, 1635, 1662, 1775, 1797, 1932, 1935
FspAI RTGCGCAY 1 cut(s) 641
FspBI CTAG 7 cut(s) 248, 278, 567, 746, 1170, 2055, 2156
FspI TGCGCA 1 cut(s) 641
GlaI GCGC 5 cut(s) 641, 1457, 1477, 1826, 2282
GluI GCNGC 8 cut(s) 252, 1408, 1635, 1662, 1775, 1797, 1932, 1935
GsaI CCCAGC 3 cut(s) 970, 1462, 2313
GsuI CTGGAG 2 cut(s) 951, 1401
HaeII RGCGCY 2 cut(s) 1459, 2284
HaeIII GGCC 7 cut(s) 28, 302, 328, 799, 1378, 1734, 1815
HapII CCGG 5 cut(s) 51, 1375, 1807, 2326, 2557
HgaI GACGC 2 cut(s) 46, 2598
HhaI GCGC 5 cut(s) 642, 1458, 1478, 1827, 2283
Hin6I GCGC 5 cut(s) 640, 1456, 1476, 1825, 2281
HinP1I GCGC 5 cut(s) 640, 1456, 1476, 1825, 2281
HincII GTYRAC 2 cut(s) 1164, 2578
HindII GTYRAC 2 cut(s) 1164, 2578
HindIII AAGCTT 5 cut(s) 305, 523, 560, 2073, 2600
HpaII CCGG 5 cut(s) 51, 1375, 1807, 2326, 2557
HphI GGTGA 7 cut(s) 122, 371, 638, 1814, 2239, 2255, 2369
Hpy166II GTNNAC 8 cut(s) 554, 828, 1164, 1216, 1655, 2246, 2399, 2578
Hpy8I GTNNAC 8 cut(s) 554, 828, 1164, 1216, 1655, 2246, 2399, 2578
Hpy99I CGWCG 2 cut(s) 62, 961
HpyAV CCTTC 5 cut(s) 362, 650, 744, 797, 1724
HpyCH4III ACNGT 7 cut(s) 80, 269, 365, 957, 1007, 1790, 1822
HpyCH4IV ACGT 2 cut(s) 600, 792
HpySE526I ACGT 2 cut(s) 600, 792
HspAI GCGC 5 cut(s) 640, 1456, 1476, 1825, 2281
Kpn2I TCCGGA 1 cut(s) 2325
KpnI GGTACC 1 cut(s) 423
KroI GCCGGC 1 cut(s) 1374
KroNI GCCGGC 1 cut(s) 1376
Ksp22I TGATCA 1 cut(s) 2382
Kzo9I GATC 7 cut(s) 395, 727, 841, 883, 947, 1555, 2382
LguI GCTCTTC 1 cut(s) 2580
LmnI GCTCC 4 cut(s) 1382, 1390, 1598, 2095
Lsp1109I GCAGC 6 cut(s) 263, 1394, 1648, 1786, 1918, 1921
LweI GCATC 9 cut(s) 25, 97, 1015, 1310, 1711, 1946, 2077, 2341, 2543
MaeI CTAG 7 cut(s) 248, 278, 567, 746, 1170, 2055, 2156
MaeII ACGT 2 cut(s) 600, 792
MaeIII GTNAC 3 cut(s) 359, 1289, 1802
MalI GATC 7 cut(s) 397, 729, 843, 885, 949, 1557, 2384
MboI GATC 7 cut(s) 395, 727, 841, 883, 947, 1555, 2382
MfeI CAATTG 3 cut(s) 333, 2000, 2026
MflI RGATCY 3 cut(s) 727, 841, 883
MhlI GDGCHC 6 cut(s) 1012, 1387, 1465, 1657, 1683, 1873
MlsI TGGCCA 2 cut(s) 28, 328
MluNI TGGCCA 2 cut(s) 28, 328
MlyI GAGTC 3 cut(s) 2352, 2427, 2573
MmeI TCCRAC 1 cut(s) 1541
Mox20I TGGCCA 2 cut(s) 28, 328
Mph1103I ATGCAT 1 cut(s) 1742
MroI TCCGGA 1 cut(s) 2325
MroNI GCCGGC 1 cut(s) 1374
MscI TGGCCA 2 cut(s) 28, 328
MseI TTAA 9 cut(s) 147, 374, 441, 519, 1124, 1943, 2472, 2568, 2624
MslI CAYNNNNRTG 4 cut(s) 1254, 1864, 2394, 2426
Msp20I TGGCCA 2 cut(s) 28, 328
MspA1I CMGCKG 1 cut(s) 1796
MspCI CTTAAG 1 cut(s) 373
MspI CCGG 5 cut(s) 51, 1375, 1807, 2326, 2557
MspR9I CCNGG 3 cut(s) 285, 1380, 2558
MunI CAATTG 3 cut(s) 333, 2000, 2026
Mva1269I GAATGC 3 cut(s) 579, 642, 1740
MvaI CCWGG 2 cut(s) 285, 1380
MvnI CGCG 1 cut(s) 57
NaeI GCCGGC 1 cut(s) 1376
NciI CCSGG 1 cut(s) 2558
NdeI CATATG 2 cut(s) 1481, 2494
NdeII GATC 7 cut(s) 395, 727, 841, 883, 947, 1555, 2382
NgoMIV GCCGGC 1 cut(s) 1374
NheI GCTAGC 1 cut(s) 247
NlaIV GGNNCC 4 cut(s) 421, 1338, 1524, 1600
NmuCI GTSAC 3 cut(s) 359, 1289, 1802
NsbI TGCGCA 1 cut(s) 641
NsiI ATGCAT 1 cut(s) 1742
NspI RCATGY 2 cut(s) 855, 2535
NspV TTCGAA 1 cut(s) 2146
OliI CACNNNNGTG 1 cut(s) 2394
PaeR7I CTCGAG 1 cut(s) 1682
PagI TCATGA 1 cut(s) 675
PciSI GCTCTTC 1 cut(s) 2580
PctI GAATGC 3 cut(s) 579, 642, 1740
PdiI GCCGGC 1 cut(s) 1376
PfeI GAWTC 9 cut(s) 127, 205, 347, 647, 679, 764, 890, 1019, 1545
PflFI GACNNNGTC 1 cut(s) 955
PkrI GCNGC 8 cut(s) 253, 1409, 1636, 1663, 1776, 1798, 1933, 1936
PleI GAGTC 3 cut(s) 2351, 2426, 2573
PpsI GAGTC 3 cut(s) 2351, 2426, 2573
PshBI ATTAAT 1 cut(s) 2624
PsiI TTATAA 1 cut(s) 231
Psp124BI GAGCTC 2 cut(s) 1387, 1683
Psp1406I AACGTT 1 cut(s) 600
Psp6I CCWGG 2 cut(s) 283, 1378
PspEI GGTNACC 2 cut(s) 359, 1802
PspFI CCCAGC 3 cut(s) 966, 1458, 2309
PspGI CCWGG 2 cut(s) 283, 1378
PspN4I GGNNCC 4 cut(s) 421, 1338, 1524, 1600
PspPI GGNCC 2 cut(s) 300, 1309
PspXI VCTCGAGB 1 cut(s) 1682
PsrI GAACNNNNNNTAC 4 cut(s) 1597, 1629, 1955, 1987
PstI CTGCAG 3 cut(s) 735, 817, 1287
PstNI CAGNNNCTG 1 cut(s) 1799
PsuI RGATCY 3 cut(s) 727, 841, 883
PsyI GACNNNGTC 1 cut(s) 955
RsaI GTAC 9 cut(s) 82, 421, 856, 876, 1199, 1265, 1876, 1988, 2129
RsaNI GTAC 9 cut(s) 81, 420, 855, 875, 1198, 1264, 1875, 1987, 2128
RseI CAYNNNNRTG 4 cut(s) 1254, 1864, 2394, 2426
SacI GAGCTC 2 cut(s) 1387, 1683
SapI GCTCTTC 1 cut(s) 2580
SaqAI TTAA 9 cut(s) 147, 374, 441, 519, 1124, 1943, 2472, 2568, 2624
SatI GCNGC 8 cut(s) 252, 1408, 1635, 1662, 1775, 1797, 1932, 1935
Sau3AI GATC 7 cut(s) 395, 727, 841, 883, 947, 1555, 2382
Sau96I GGNCC 2 cut(s) 300, 1309
ScaI AGTACT 1 cut(s) 82
SchI GAGTC 3 cut(s) 2352, 2427, 2573
ScrFI CCNGG 3 cut(s) 285, 1380, 2558
SduI GDGCHC 6 cut(s) 1012, 1387, 1465, 1657, 1683, 1873
SfaNI GCATC 9 cut(s) 25, 97, 1015, 1310, 1711, 1946, 2077, 2341, 2543
SfcI CTRYAG 5 cut(s) 731, 813, 1283, 1897, 2262
Sfr274I CTCGAG 1 cut(s) 1682
SfuI TTCGAA 1 cut(s) 2146
SinI GGWCC 1 cut(s) 1309
SlaI CTCGAG 1 cut(s) 1682
SmiMI CAYNNNNRTG 4 cut(s) 1254, 1864, 2394, 2426
SmlI CTYRAG 4 cut(s) 200, 373, 1682, 2413
SmoI CTYRAG 4 cut(s) 200, 373, 1682, 2413
SsiI CCGC 2 cut(s) 1634, 1796
SspI AATATT 1 cut(s) 773
SspMI CTAG 7 cut(s) 248, 278, 567, 746, 1170, 2055, 2156
SstI GAGCTC 2 cut(s) 1387, 1683
StyD4I CCNGG 3 cut(s) 283, 1378, 2556
StyI CCWWGG 2 cut(s) 683, 700
TaaI ACNGT 7 cut(s) 80, 269, 365, 957, 1007, 1790, 1822
TaiI ACGT 2 cut(s) 603, 795
TaqI TCGA 7 cut(s) 511, 901, 950, 1180, 1683, 2146, 2517
TatI WGTACW 2 cut(s) 80, 2127
TauI GCSGC 2 cut(s) 1637, 1799
TfiI GAWTC 9 cut(s) 127, 205, 347, 647, 679, 764, 890, 1019, 1545
Tru1I TTAA 9 cut(s) 147, 374, 441, 519, 1124, 1943, 2472, 2568, 2624
Tru9I TTAA 9 cut(s) 147, 374, 441, 519, 1124, 1943, 2472, 2568, 2624
TseFI GTSAC 3 cut(s) 359, 1289, 1802
TseI GCWGC 6 cut(s) 251, 1407, 1661, 1774, 1931, 1934
Tsp45I GTSAC 3 cut(s) 359, 1289, 1802
Tth111I GACNNNGTC 1 cut(s) 955
Vha464I CTTAAG 1 cut(s) 373
VneI GTGCAC 1 cut(s) 1653
VpaK11BI GGWCC 1 cut(s) 1309
VspI ATTAAT 1 cut(s) 2624
XagI CCTNNNNNAGG 1 cut(s) 1422
XapI RAATTY 4 cut(s) 184, 897, 2175, 2431
XbaI TCTAGA 1 cut(s) 1169
XceI RCATGY 2 cut(s) 855, 2535
XcmI CCANNNNNNNNNTGG 1 cut(s) 865
XhoI CTCGAG 1 cut(s) 1682
XspI CTAG 7 cut(s) 248, 278, 567, 746, 1170, 2055, 2156
ZrmI AGTACT 1 cut(s) 82
Zsp2I ATGCAT 1 cut(s) 1742
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.