Rorug04G0335000

Pentatricopeptide repeat-containing protein At2g01510

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0013352
Physical Location & Seq
Forward (+)
50928095 .. 50928448
354 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0335000.1

Sequence Viewer

Length: 354 bp
ATGGAAGTAGGGAATCATTCCGGGTCTGCAAATACTACAGAAATTGAAATGGTTGAAGATCGGGAACGAGATCCGGAGACAAACAGGGTGGTGTCACATAGTTGTGGGGAAGGACGATGTCAAGCGTTAACTTCCAACTATGGAGAGGCATCCAAGAGAAACCTGATGACAGCACTCGTAGCCTCAGTCATTTTCATGACAGTGGAAATTGTTGTAGGCATCAAAGCCAATAGTTTGGCAATTCGGTCCGATGCGCTACATGTCTTAACAGATGTTGTGGCATTTGCAATTTCTGTGTTCTCAGTTTGGGCATCGGGGTGGAAGGCAACTCCAGGCAAGACTTATGGCTTCTGA

Protein Analysis

117

Amino Acids

12.59

Weight (kDa)

5.53

Isoelectric Point (pI)

20.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cation_efflux PF01545 56 - 117 7e-11 Cation efflux transmembrane domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 73
AclWI GGATC 1 cut(s) 65
AflIII ACRYGT 1 cut(s) 259
AgsI TTSAA 2 cut(s) 47, 56
AjnI CCWGG 1 cut(s) 331
Alw26I GTCTC 1 cut(s) 71
AlwI GGATC 1 cut(s) 65
Aor13HI TCCGGA 1 cut(s) 73
AspLEI GCGC 1 cut(s) 256
AspS9I GGNCC 1 cut(s) 246
AsuC2I CCSGG 1 cut(s) 22
AvaII GGWCC 1 cut(s) 246
BciT130I CCWGG 1 cut(s) 333
BcnI CCSGG 1 cut(s) 22
BcoDI GTCTC 1 cut(s) 71
BfmI CTRYAG 1 cut(s) 36
Bme1390I CCNGG 2 cut(s) 22, 333
Bme18I GGWCC 1 cut(s) 246
BmgT120I GGNCC 1 cut(s) 246
BmrFI CCNGG 2 cut(s) 22, 333
BmsI GCATC 4 cut(s) 158, 228, 241, 320
BpmI CTGGAG 1 cut(s) 315
BpuMI CCSGG 1 cut(s) 22
BsaWI WCCGGW 1 cut(s) 73
BseAI TCCGGA 1 cut(s) 73
BseBI CCWGG 1 cut(s) 333
BseGI GGATG 1 cut(s) 149
BseMII CTCAG 2 cut(s) 198, 315
BsiSI CCGG 2 cut(s) 21, 74
BsmAI GTCTC 1 cut(s) 71
Bsp13I TCCGGA 1 cut(s) 73
Bsp143I GATC 2 cut(s) 58, 70
BspCNI CTCAG 2 cut(s) 197, 314
BspEI TCCGGA 1 cut(s) 73
BspHI TCATGA 1 cut(s) 195
BspPI GGATC 1 cut(s) 65
BssMI GATC 2 cut(s) 58, 70
Bst2UI CCWGG 1 cut(s) 333
Bst4CI ACNGT 1 cut(s) 202
BstDEI CTNAG 2 cut(s) 184, 301
BstF5I GGATG 1 cut(s) 149
BstHHI GCGC 1 cut(s) 256
BstKTI GATC 2 cut(s) 61, 73
BstMAI GTCTC 1 cut(s) 71
BstMBI GATC 2 cut(s) 58, 70
BstMWI GCNNNNNNNGC 1 cut(s) 179
BstNI CCWGG 1 cut(s) 333
BstNSI RCATGY 1 cut(s) 263
BstSCI CCNGG 2 cut(s) 20, 331
BstSFI CTRYAG 1 cut(s) 36
BstX2I RGATCY 1 cut(s) 70
BstXI CCANNNNNNTGG 1 cut(s) 235
BstYI RGATCY 1 cut(s) 70
BtsCI GGATG 1 cut(s) 149
BtsIMutI CAGTG 1 cut(s) 207
CciI TCATGA 1 cut(s) 195
CfoI GCGC 1 cut(s) 256
Cfr13I GGNCC 1 cut(s) 246
CpoI CGGWCCG 1 cut(s) 246
CspCI CAANNNNNGTGG 2 cut(s) 69, 104
CspI CGGWCCG 1 cut(s) 246
CviAII CATG 2 cut(s) 196, 260
CviJI RGCY 3 cut(s) 182, 227, 348
CviKI_1 RGCY 3 cut(s) 182, 227, 348
DdeI CTNAG 2 cut(s) 184, 301
DpnI GATC 2 cut(s) 60, 72
DpnII GATC 2 cut(s) 58, 70
Eco47I GGWCC 1 cut(s) 246
EcoRII CCWGG 1 cut(s) 331
FaeI CATG 2 cut(s) 199, 263
FaiI YATR 5 cut(s) 99, 141, 197, 261, 345
FatI CATG 2 cut(s) 195, 259
FokI GGATG 1 cut(s) 136
GlaI GCGC 1 cut(s) 255
GsuI CTGGAG 1 cut(s) 315
HapII CCGG 2 cut(s) 21, 74
HhaI GCGC 1 cut(s) 256
Hin1II CATG 2 cut(s) 199, 263
Hin6I GCGC 1 cut(s) 254
HinP1I GCGC 1 cut(s) 254
HincII GTYRAC 1 cut(s) 129
HindII GTYRAC 1 cut(s) 129
HinfI GANTC 1 cut(s) 13
HpaI GTTAAC 1 cut(s) 129
HpaII CCGG 2 cut(s) 21, 74
Hpy166II GTNNAC 1 cut(s) 129
Hpy188I TCNGA 2 cut(s) 250, 353
Hpy188III TCNNGA 3 cut(s) 62, 74, 196
Hpy8I GTNNAC 1 cut(s) 129
HpyAV CCTTC 2 cut(s) 104, 316
HpyCH4III ACNGT 1 cut(s) 202
HpyCH4V TGCA 2 cut(s) 29, 287
HpyF10VI GCNNNNNNNGC 1 cut(s) 179
HpyF3I CTNAG 2 cut(s) 184, 301
Hsp92II CATG 2 cut(s) 199, 263
HspAI GCGC 1 cut(s) 254
Kpn2I TCCGGA 1 cut(s) 73
KspAI GTTAAC 1 cut(s) 129
Kzo9I GATC 2 cut(s) 58, 70
LpnPI CCDG 6 cut(s) 34, 70, 87, 176, 318, 345
LweI GCATC 4 cut(s) 158, 228, 241, 320
MaeIII GTNAC 1 cut(s) 93
MalI GATC 2 cut(s) 60, 72
MboI GATC 2 cut(s) 58, 70
MboII GAAGA 1 cut(s) 68
MflI RGATCY 1 cut(s) 70
MluCI AATT 4 cut(s) 42, 207, 240, 288
MmeI TCCRAC 1 cut(s) 159
MnlI CCTC 2 cut(s) 139, 193
MroI TCCGGA 1 cut(s) 73
MseI TTAA 2 cut(s) 128, 266
MslI CAYNNNNRTG 4 cut(s) 102, 194, 200, 316
MspI CCGG 2 cut(s) 21, 74
MspR9I CCNGG 2 cut(s) 22, 333
MvaI CCWGG 1 cut(s) 333
MwoI GCNNNNNNNGC 1 cut(s) 179
NciI CCSGG 1 cut(s) 22
NdeII GATC 2 cut(s) 58, 70
NlaIII CATG 2 cut(s) 199, 263
NmuCI GTSAC 1 cut(s) 93
NspI RCATGY 1 cut(s) 263
PagI TCATGA 1 cut(s) 195
PciI ACATGT 1 cut(s) 259
PfeI GAWTC 1 cut(s) 13
PflFI GACNNNGTC 1 cut(s) 117
PscI ACATGT 1 cut(s) 259
Psp6I CCWGG 1 cut(s) 331
PspGI CCWGG 1 cut(s) 331
PspPI GGNCC 1 cut(s) 246
PsuI RGATCY 1 cut(s) 70
PsyI GACNNNGTC 1 cut(s) 117
RseI CAYNNNNRTG 4 cut(s) 102, 194, 200, 316
Rsr2I CGGWCCG 1 cut(s) 246
RsrII CGGWCCG 1 cut(s) 246
SaqAI TTAA 2 cut(s) 128, 266
Sau3AI GATC 2 cut(s) 58, 70
Sau96I GGNCC 1 cut(s) 246
ScrFI CCNGG 2 cut(s) 22, 333
SetI ASST 1 cut(s) 165
SfaNI GCATC 4 cut(s) 158, 228, 241, 320
SfcI CTRYAG 1 cut(s) 36
SinI GGWCC 1 cut(s) 246
SmiMI CAYNNNNRTG 4 cut(s) 102, 194, 200, 316
Sse9I AATT 4 cut(s) 42, 207, 240, 288
StyD4I CCNGG 2 cut(s) 20, 331
TaaI ACNGT 1 cut(s) 202
TaqII GACCGA 1 cut(s) 234
TasI AATT 4 cut(s) 42, 207, 240, 288
TfiI GAWTC 1 cut(s) 13
Tru1I TTAA 2 cut(s) 128, 266
Tru9I TTAA 2 cut(s) 128, 266
TscAI CASTG 1 cut(s) 207
TseFI GTSAC 1 cut(s) 93
Tsp45I GTSAC 1 cut(s) 93
TspDTI ATGAA 1 cut(s) 184
TspRI CASTG 1 cut(s) 207
Tth111I GACNNNGTC 1 cut(s) 117
VpaK11BI GGWCC 1 cut(s) 246
XceI RCATGY 1 cut(s) 263
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.