Rorug04G0337700
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0009054
Physical Location & Seq
Forward (+)
51137931 .. 51148767
10837 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0337700.1

Sequence Viewer

Length: 7236 bp
ATGTCTGAGAAGAGCGATTCCGGCCAGCTTCCGGACGGTGCTGCTGCGGCTGCAGATTCGAATGCGATTTGGAACGACGTCGTTTCACCGGCGAACAAAACCGAGGAATTGGGGAAAGTGTTGGAGGACGCGGGGTCAGGGAAGGAGGACATGTTCGAAGATTGCCCCGACGATATCTTGGTGGATGAGGTCGAACGTTTACGACTTCAGCTGGATGCTAGTATTGCAGAGAAAGATGACTTTGCTCGCAAATTTAAGGAAGAGAGAGAGTCTTTTGTTAGAGAAGTTGGTACTCTTCGAATTCAGCTTAGGGGTCTCACTGATCAACAGCCATTGGTGGGTGAAAATGGCGGTGATTTTTGTAATAATGAGGCTGAAAGTGGAGAGAATGGAGAAAAGACTGCAGTGGTGGACAAGGGTACACCGTGGATTGACTTGATAAAGGAGTGTTCTGGGTTTGTTAAGAAGGCGTTGGAGAAACAGTCTCAGATTGAGGCCAGGGTGAGAGAGCTCGATGGAATTGTGTATATGAAGGATCAAGAAATCGAGGGTCTCAATGCCAATGTGAAAGTTTTATATGAGAGTCACCATGAGAAGGATGCATATTTTGAAGCTCTGGCAAATAGGATGTTGGCTTCCCTTAGCCGTGTAGTTGGTCAACAAGAACTGCTGGATGATTCTATTGGTGGAAAACTAGTACATGTTGAGAATGGCACGTCTATGTTAGTTGAGAATTTCACCCGGAAGCTTTCTGAAATTGAACATTTCAGACAGTGTTTGCCTGAGACCGGGTTTGATCATAGTTCGCAGGAGGTGGGGGGCATTTTTGCTGCTGCTCGTAATGAGTTACTTGAGCTCAGAAGAAAGGAAGCCGAATTTGTTGAAAGACTGAGTCTTCTAGAAGATTCAAATAGGAAATTGGTTGAAGAACTAGACAATCAGAGAGCGATAGCTGAAAGGCTGAATGCAGAACTTGGACAAACAAAAATGGAGCTGGAGCAGGAGAAGAATAGGTGTGCTAATACTAGAGAGAAGCTTACTATCGCTGTGCAGAAAGGCAAGGGATTGGTACATCAAAGAGACTCATTGAAGCAGTCCATTGCTGAAAAACTGAGTGAGCTTGAGAAGTGTCGGACTGAATTGCAGGAGAAGTCAAGTGCTCTTGAAGCTGCTGAATTAAGCAAGGAGGAATTGATTAGGAGTGAAAACTCAGTTGCATCACTGCAAGAAACACTTTCCCAAAATAATGTAATTCTTCAGAAACTTGAAGAAATGTTGTCTCAGACTGGTGTACCTGAAGAGCTTCAATCTATGGATATTGTAGAGAGACTTCGATGGCTTGTGGATGAAAGTGTTAAACTCAAGGAAATTTCTATGGAATTCCAAACCTTGAAAGATGCTATGTATGCTATTGGTCTACCAGAAGTTATTTCATCTTCTAGTTTGGAATCTCAAATAAGTTGGCTTCGGGAATCATATTCTCAGGCGAATGAGGAAGTGCTTGTGCTACATAATGAAATTACTGCAACTAAGGAAGTGGCACATAAAAATATTGACCAGTTAACTGAATCACTTTCAGCTGAATTGCGGGCAAAAGAACATCTCCAAGCAGAGTTGGACAACATAACATCGGAATACAATGAGATTATCAAGAAAGAGCATCAAGTGTCTTTAGAGAAAGCTCAGATGGTCAGAAGGTTACTTGATGTGTCTGGAGTTGTAATGGACAATGAGGATGTCTCTCAACTTTCCTCAGACATTGCCACGCTCATTGACACATGTATTGAGAAGATAAAAGAACAGAGCTCTGCTTCACTTTCGGCTGAAATGCAGGCAAAGGAAGTTCTCCAAGTGGAGTTTGACAGTCTTACATCAAAGTACAAAGAGATGGTTGAAAAGGAGCGTCAGGTATCTTCAGAGAATGCTGAGATGGTCAAAATGTTACTTGATGTCTCTGGAATTGTGATTGACAATGAGGATGTCTCTCAGCTTTCCTCAGACATTGGCACGTTCATTGACACATGTATTGGGAAGATAAAAGAACAGAGCAGTGCTTCATTTGAACAGTCAACTGCTTCGCTTTCAGCTGAAATGCAAGCAAAGGAATATCTCCAAGTAGAGTTGGAGAGTCTAACATCGAAGTACAAAGAGATTGTTGAGAAAGAGCGTCAAGTGTCTTCAGAGAAGGCTGAGATGGTCAAAATGTTACTTGATGTCTCTGGAATTGTGATTGACAATGAAGATGTCTCTCAGCTTTCCTCAGACACTGCCACGCTCATTGATAGATGCGTTCAGAAGATAAAAGAACAGAGCAGTGCCTCACTTGATTATCCAAGTTTCAATGTGGAGCTATTTGAAACAATTCAAAGCCACTTGTACGTAAGAGATCAGGAGTTGATGCTCTGTCGGAATATTCTAGAAGAGGAGATGGTGATGAAATCAGAGGTGAATAAACTGTCGGAGGAATTACGAATTGTGTCTCAACAAGTTGAGGCATTGAAGGAGGAAAAAGGGTCCCTACAGAGAGATATTGAACGGTCAGAACAGGAGAATGCTATGATAAGGGAGAAGTTATCCGTGGCAGTCAAGGAAGGTAAGGAAATGGTTCAAGAGCGGGGAAATGCTTCATTTGAACAGTTAACTGCTTCTCTTTCAGCTGAAATGCAGGCAAAGGAATATCTCCAAGTAGAGTTGGAGAATCTAACATTGAAGTACAAAGAGATTGTTGAGAAAGAGCGTCAAGTGTCTTCAGAGAAGGCTGAGATGGTCAAAATGTTACTTGATGTCTCTGGAATTGTGATTGACAATGAAGATGTCTCTCAGCTTTCCTCAGACACTGCCACGCTCATTGATAGATGCATTCAGAAGATAAAAGAACAGAGCAGTGCCTCACTTGATTCTCCAAGTTTCGATGTGGAGCTATTTGAAACAATTCAAAGCCACTTGTACGTAAGAGATCAGGAGTTGATGCTCTGTCAGAATATTCTAGAAGAGGAGATGGTGGTGAAATCAGAGGTGAATAAACTGTCGGAGGAATTACTAATTGTGTCTCAACAAGTTGAGGCATTGAAGGAGGAAAAAGGGTCCCTACAGAGAGATATTGAACGGTCAGAAGAGAAGAATGCTATGATAAGGGAGAAGTTATCCATGGCAGTCAAGAAAGGTAAGGGAATGGTTCAAGAGCGGGAAAACTTGAAACTTCGTGTGGAAGAAAAGAACTCGGAGATTGAGAAGCTAAGGCTTGAGTTACAGCAGGAACAATCCACACTGTCTGAATGCAGGGACAAGATAAATAGCTTGTCTGCTGATACAGACTGCATTCCAAAGTTAGAAGCTGATCTTGTTGCTATGAAGGAACAAAGGGATCAACTCGAACAGTTCTTACTGGAGAGCAATAACATGTTACAGAGAGTAACTAAAGCAATTGATGCAATCGTGCTTCCTGTTGATTCTTTTGAAGAACCTTTGGGGAAGGTGAACTGGCTTGCAGGATACCTGAGTGAATGTCATGATGCTGAGGCAAAAGCAAAGCAAGAGTTGGGAAAAGTACAAGAGGAAACAAGTAATCTTGTGTTTAAGTTGGAAGAAGCCCATTCAACAATAAATTCACTGGAAAATGAATTATCAGTGGCGGAGAATAGTTTGTCACAACTTGCTGAAGAAAAGAGGGAAATGGAAGTCAGTAAGACCAGTGTTGAAAAAGAGTTACAGAGAGCATTAGAAGAAGCTGTTTCCCAGGCTAGCAAGTTTAGTGAGGTTTCTGCAGTTAAAAAGTCGCTGGAAGAGGCATTATCACTTGCAGAAAATAATATATCTGTTCTTGTCAGTGAAAAAGAGGGGGCTTTAGTCAGTAGAGCCACTGCAGATACAGAGCTAGGAAAGGTCAAAGAGGAAGTTGATATACAGACCAGCAAACTAATAGATGCCTACAAGACCATAGAGTCACTTGAAGTTGCACTTTCTCAGGTACAGGCCAACGTTTCTTTACTTACTGAACAAAACAATGATGCTCAAATTGGTAGATCCAATTTAGAGGCTGAGCTAAAGAAACTGCAAGAGGAAGCTAGGTTGCAGGATAACAAGCTTGCAGATGCCAGTGCAACCATAAATAAACTGGAAGATGCACTGTTGAAAGCAGGGAATGATATATCTGAACTTGAAACTGGAAAGAAAAATGCTGAAGAGGAGATATTAAAACTCAGTTCCAAGTTAAATGCATCCATCGAAGAGTTGCCTGGAACTAATGGCAACACAGAAAACAGGTCCTTAGAGCTCACTGGTCACCTTGATAATCTTCAAGTGCTAATGAAGGACGAGACTATGTTGTCCACAATGAAAAGATGTTTTGAGAAGAAGTTTGAGAGCCTGAAAGATATGGATCTCATTCTTAAAAATATAAGAGACCAGTGTGTTTCAGGGGGTTTAGCTGAGCTGCAAGGTCAGCAAGTACTGGAGGAAGATTCATATGTGACCAAATCTTTTTCAGATGGTCTTGTCAATATTGTCAGTGTTGAAAAGGACAATGCTGAGGTCAATGGTGCAGATGGTAATGATATACCTTCATATTTGAAGACGACTGTGGAAAGGTTGCAGTTGCGCGACATGGTTCTGTCACAAAATTTTGAACGCTTCTCTTCTTTTATAGATGAGTTTATTGAAACTCTGTTAAGAAATTTACAGGCACGAAGTGATGAAGTAGCAGTTATGTTCGAGCACATGCAGTCTTTTAAACAAAAAGCAAATAATTTGGAAATATATAAACATGAACAGGAGAATACCATAGCCATTTTAGAAAATGATCTCAAGTCTCTTGTCTCTGCTTGTACTGATGCGACCAGAGAACTGCAATTTGAAGCTAAGAACAAGTTACTTGAACTCAGCTCTGTTCCTGAACTTGAGGAATTGAGACATAATTTGCCCCAAGAAACAGGAGCAACTGATGGAGAGACCACGGAAACACATGGGCAAGGTATTGATGACAGCAACTATGGTAAAACAGCAGGAATGTTGTCAGTTGCTTGCAGAAATGTTCAAGCTTTGGTCAGACAGTTTGAGATCACAAGTAAAGTGGCCGCGTCTACAATTGAAGATTTGCAGAATAAACTGAAAGAAGCTAGAACTACTTCTGAAAAAGCCATTGAAGAAAGGGATCTAAGACAAAATAGGATTTCCAAGTTGGAGGCGGATATATATGCGTTAGAAAGTTCTTGCACTAATCTGACGCTTAAACTAGAGGATTATCAAGCAAAAGAGGATAGATTGAAGGAAAGGGAGGCAGAACTTTCATCACTACACAATACTTTGTCAATGAAAGAACAAGAGAATGAAGACTCCCTCCTATCAGCATCAGAAGTCAAAATCCTCTTCGACAAAATCAAAGGGATTGAAATCCCTATACCAGAGTCAGAAGTAGGAGACTTAGAATCCCATAATTCAACTCATGTGAAGAAGCTCTTTTGCGTTATCGATAACATTAGTAACTTTCAGCATCAGATAAACTCACTGTCTTCCGAAAAAGAAGAGCTACAGTCAACACTCCGAACGCAATTTCTTGAAATAGAGCATCTAAAGGAGGAGGTGGAAAACCATGTCAGAGATGGACAAGATACTGAGAAGATGAAAAATGAACTGTCTGTGCTGATATATGCTTTGCAAAAAATTATAGATATGTCGGGAGGTGATGATTTAGTTGGAGACGAGAAGTCTGCTGGTGTGAAGGGACTTGTGTCGGTGTTAGAGAAGCAGGTTATGGCTTTGCTACTTGAATCTAAAAATTCAAAATCAAAAGCTCAGGAACTTGGCACTAAGTTGGTCGAGAGCCAAAAGGTTGTGGATGAATTATCAACCAAGGTTAATTTACTTGAAGTTTCAGCTCAAGGAAGGGTTGCTCAGCCAGAGATTGTCCAGGAAAGGAGCATCTTTGAAGCACCTTCCTTGCCTACTGGTTCAGAGATATCTGAAATTGAAGATGTGGGATCACTTGGAAGCAAAACGATATCCCCTGTTCCATCAGCAGCTCATGTGCGCATGATGCGAAAGGGTTCCACTGACCATCTTGCAATTGATATCGATCCCGAATCTACTCGTTTAATCAGCACTGAAGAAACTGACGAGGACAAAGGCCATGTATTCAAGTCTCTCAATGCATCTGGTATCATTCCAAGACAAGGAAAGTTGATTGCAGATCGAATTGATGGAATTTGGGTATCTGGTGGTCGAAGTTTGATGAGTCGCCCAAGGGCTAGGCTAGGCGTCATTGCCTACTGGCTTGTCTTGCATCTGTGGCTGCTAGGAGTCATCATTCTCAGGCATCAAAGAGCACCCATTAAACAAATGGGTTGGCTTACAGTAAACAATGCATCTACAACCTCATTGACTTGGGGGAACTCTATCTTCACTTGTGGGAAGTGTATTGGCCTCTATGATGTGCAAAAGTGTTTACCAGCCAAATCATGCAGTGGAGTTGCATTTAGCCCAAGAGCTTTTGCAAGCCGGAATTCGGTGAAGAAGTTGAGAAGGGAGAGACTAGCAAGCGAAAGGGTAGCAGATAAAACTACTGCTAGTTCTGAAAATGTTCAAAATGACAACAAGGTGGAGCTTTCTGATGATTCAGCTGCAGAGAAAGGGGTTAATGTCCCTTCCAGAGGTGCTGTGCTTCAGGCTTGCACTGTTACTTCCGGGTTGATTGCTGCTTTGGGTATACTAATTCGACAGGCATCTCATGTTGCATCGGTTGAAGGATTACCAGTCCTTGACTGCTCATTGGAAGTATCATTTGATTTTGAGATGTGGCATCTTCAGTTGATTGCCGGATTGGTTATTTTGATATCATCATCCCGATATATATTACTGAAGACATGGCCAGAATTTGCAGAGTCTAGCAAAGCAGCCAATCAACAGGTCCTTACTTCACTTCAACCTTTGGATTTCATAATCGTTGCATTTTTACCTGGGGTCAGCGAGGAACTTCTTTTCCGGGGTGCGCTGCAGCCTCTTTTTGGATCCAATTTGAGTAGTGCATTGGTGGTTGCCCTCGTCTTTGGTGTTCTACACCTGGGCAGTGGCCGAAAGTATTCCTTCGCAGTCTGGGCAAGTTTGGTTGGGTTTGTGTATGGTTACGCAGCCATCGCATCTTCTAGCCTTGTTGTGCCAATGGTTTCTCATGCAGTGAACAATTTGGTGGGAGGGATTTTATGGCGATACAGATCAGATTCATCAGGAGAGATATCAGAGTAA

Protein Analysis

2411

Amino Acids

268.98

Weight (kDa)

4.77

Isoelectric Point (pI)

47.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rce1-like PF02517 2306 - 2394 2.3e-16 Type II CAAX prenyl endopeptidase Rce1-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 3940, 4324
AatII GACGTC 1 cut(s) 81
Acc16I TGCGCA 1 cut(s) 6026
Acc36I ACCTGC 1 cut(s) 5734
AccBSI CCGCTC 2 cut(s) 2614, 3184
AccI GTMKAC 3 cut(s) 1417, 5078, 6700
AccII CGCG 3 cut(s) 131, 4599, 5075
AccIII TCCGGA 1 cut(s) 31
AciI CCGC 9 cut(s) 47, 131, 351, 1588, 2614, 3184, 3632, 5073, 5183
AclI AACGTT 2 cut(s) 196, 3978
AclWI GGATC 9 cut(s) 543, 3372, 4017, 4386, 5157, 5983, 6065, 6996, 7009
AcoI YGGCCR 4 cut(s) 22, 5070, 6860, 7063
AcyI GRCGYC 2 cut(s) 78, 6252
AdeI CACNNNGTG 1 cut(s) 4688
AfiI CCNNNNNNNGG 8 cut(s) 31, 338, 595, 788, 6167, 6499, 6644, 6998
AflIII ACRYGT 5 cut(s) 150, 700, 1778, 2021, 3399
AhdI GACNNNNNGTC 2 cut(s) 133, 5701
AhlI ACTAGT 1 cut(s) 694
AjiI CACGTC 1 cut(s) 717
AjnI CCWGG 6 cut(s) 497, 3735, 4234, 5904, 6949, 7053
AloI GAACNNNNNNTCC 4 cut(s) 137, 169, 6957, 6989
Alw21I GWGCWC 7 cut(s) 513, 858, 1162, 1808, 4275, 4716, 6322
AlwI GGATC 9 cut(s) 543, 3372, 4017, 4386, 5157, 5983, 6065, 6996, 7009
AlwNI CAGNNNCTG 2 cut(s) 2267, 2837
Aor13HI TCCGGA 1 cut(s) 31
AoxI GGCC 8 cut(s) 22, 495, 3972, 5070, 6121, 6415, 6860, 7063
Asp700I GAANNNNTTC 9 cut(s) 1302, 2361, 2604, 2623, 2931, 5122, 6040, 6573, 7072
AspLEI GCGC 3 cut(s) 4599, 6027, 6985
AspS9I GGNCC 4 cut(s) 2514, 3084, 4263, 6901
AsuC2I CCSGG 4 cut(s) 742, 790, 6679, 6977
AsuII TTCGAA 3 cut(s) 59, 156, 298
AsuNHI GCTAGC 1 cut(s) 3740
AvaII GGWCC 4 cut(s) 2514, 3084, 4263, 6901
BaeI ACNNNNGTAYC 4 cut(s) 1275, 1308, 3484, 3517
BalI TGGCCA 1 cut(s) 6862
BamHI GGATCC 1 cut(s) 7001
BanII GRGCYC 4 cut(s) 513, 858, 1808, 4275
BarI GAAGNNNNNNTAC 4 cut(s) 1314, 1346, 3885, 3917
BbsI GAAGAC 7 cut(s) 887, 2169, 2739, 4577, 5334, 5499, 6860
Bbv12I GWGCWC 7 cut(s) 513, 858, 1162, 1808, 4275, 4716, 6322
BbvCI CCTCAGC 2 cut(s) 3516, 4527
BceAI ACGGC 1 cut(s) 630
BcgI CGANNNNNNTGC 2 cut(s) 5687, 5721
BciT130I CCWGG 6 cut(s) 499, 3737, 4236, 5906, 6951, 7055
BciVI GTATCC 1 cut(s) 3485
BclI TGATCA 2 cut(s) 322, 796
BcnI CCSGG 4 cut(s) 742, 790, 6679, 6977
BcuI ACTAGT 1 cut(s) 694
BfmI CTRYAG 9 cut(s) 51, 402, 2519, 3089, 3762, 3861, 5525, 6615, 6986
BfuAI ACCTGC 1 cut(s) 5734
BfuI GTATCC 1 cut(s) 3485
BlpI GCTNAGC 3 cut(s) 4038, 4428, 5889
BmcAI AGTACT 1 cut(s) 4449
Bme18I GGWCC 4 cut(s) 2514, 3084, 4263, 6901
BmeRI GACNNNNNGTC 2 cut(s) 133, 5701
BmgBI CACGTC 1 cut(s) 717
BmgT120I GGNCC 4 cut(s) 2514, 3084, 4263, 6901
BmiI GGNNCC 6 cut(s) 2515, 2516, 3085, 3086, 6043, 7003
BmtI GCTAGC 1 cut(s) 3744
BoxI GACNNNNGTC 1 cut(s) 5725
BpiI GAAGAC 7 cut(s) 887, 2169, 2739, 4577, 5334, 5499, 6860
BplI GAGNNNNNCTC 4 cut(s) 1724, 1756, 1967, 1999
BpmI CTGGAG 4 cut(s) 1016, 1734, 3407, 4472
Bpu10I CCTNAGC 6 cut(s) 308, 641, 3236, 3516, 4527, 5790
Bpu1102I GCTNAGC 3 cut(s) 4038, 4428, 5889
Bpu14I TTCGAA 3 cut(s) 59, 156, 298
BpuEI CTTGAG 7 cut(s) 872, 1142, 1346, 3263, 4787, 4916, 5859
BpuMI CCSGG 4 cut(s) 742, 790, 6679, 6977
Bsa29I ATCGAT 2 cut(s) 5466, 6069
BsaAI YACGTR 2 cut(s) 2380, 2950
BsaBI GATNNNNATC 4 cut(s) 4377, 5387, 6069, 7204
BsaHI GRCGYC 2 cut(s) 78, 6252
BsaI GGTCTC 5 cut(s) 320, 557, 779, 4395, 4940
BsaWI WCCGGW 1 cut(s) 31
BsaXI ACNNNNNCTCC 4 cut(s) 137, 167, 467, 497
Bsc4I CCNNNNNNNGG 8 cut(s) 31, 338, 595, 788, 6167, 6499, 6644, 6998
Bse118I RCCGGY 1 cut(s) 88
Bse3DI GCAATG 3 cut(s) 1098, 1758, 6255
Bse8I GATNNNNATC 4 cut(s) 4377, 5387, 6069, 7204
BseAI TCCGGA 1 cut(s) 31
BseBI CCWGG 6 cut(s) 499, 3737, 4236, 5906, 6951, 7055
BseCI ATCGAT 2 cut(s) 5466, 6069
BseJI GATNNNNATC 4 cut(s) 4377, 5387, 6069, 7204
BseLI CCNNNNNNNGG 8 cut(s) 31, 338, 595, 788, 6167, 6499, 6644, 6998
BseMI GCAATG 3 cut(s) 1098, 1758, 6255
BseRI GAGGAG 4 cut(s) 2438, 3008, 4199, 5588
BsgI GTGCAG 2 cut(s) 1070, 4560
Bsh1236I CGCG 3 cut(s) 131, 4599, 5075
BshFI GGCC 8 cut(s) 24, 497, 3974, 5072, 6123, 6417, 6862, 7065
BshVI ATCGAT 2 cut(s) 5466, 6069
BsiHKAI GWGCWC 7 cut(s) 513, 858, 1162, 1808, 4275, 4716, 6322
BsiSI CCGG 9 cut(s) 21, 32, 89, 742, 789, 6493, 6678, 6810, 6976
BslFI GGGAC 5 cut(s) 2500, 3070, 3296, 5733, 6620
BslI CCNNNNNNNGG 8 cut(s) 31, 338, 595, 788, 6167, 6499, 6644, 6998
BsmBI CGTCTC 1 cut(s) 5688
BsmFI GGGAC 5 cut(s) 2500, 3070, 3296, 5733, 6620
BsmI GAATGC 8 cut(s) 67, 970, 1927, 2557, 2859, 3127, 3281, 3318
BsnI GGCC 8 cut(s) 24, 497, 3974, 5072, 6123, 6417, 6862, 7065
Bso31I GGTCTC 5 cut(s) 320, 557, 779, 4395, 4940
Bsp119I TTCGAA 3 cut(s) 59, 156, 298
Bsp1286I GDGCHC 7 cut(s) 513, 858, 1162, 1808, 4275, 4716, 6322
Bsp13I TCCGGA 1 cut(s) 31
Bsp1720I GCTNAGC 3 cut(s) 4038, 4428, 5889
Bsp19I CCATGG 1 cut(s) 3147
BspACI CCGC 9 cut(s) 47, 131, 351, 1588, 2614, 3184, 3632, 5073, 5183
BspANI GGCC 8 cut(s) 24, 497, 3974, 5072, 6123, 6417, 6862, 7065
BspDI ATCGAT 2 cut(s) 5466, 6069
BspEI TCCGGA 1 cut(s) 31
BspFNI CGCG 3 cut(s) 131, 4599, 5075
BspHI TCATGA 1 cut(s) 3508
BspLI GGNNCC 6 cut(s) 2515, 2516, 3085, 3086, 6043, 7003
BspMAI CTGCAG 6 cut(s) 55, 406, 3766, 3865, 6619, 6990
BspMI ACCTGC 1 cut(s) 5734
BspOI GCTAGC 1 cut(s) 3744
BspPI GGATC 9 cut(s) 543, 3372, 4017, 4386, 5157, 5983, 6065, 6996, 7009
BspQI GCTCTTC 3 cut(s) 5, 1293, 5514
BspT104I TTCGAA 3 cut(s) 59, 156, 298
BspTNI GGTCTC 5 cut(s) 320, 557, 779, 4395, 4940
BsrBI CCGCTC 2 cut(s) 2614, 3184
BsrDI GCAATG 3 cut(s) 1098, 1758, 6255
BsrFI RCCGGY 1 cut(s) 88
BssAI RCCGGY 1 cut(s) 88
BssNAI GTATAC 1 cut(s) 6701
BssNI GRCGYC 2 cut(s) 78, 6252
BssT1I CCWWGG 3 cut(s) 3147, 5847, 6236
Bst1107I GTATAC 1 cut(s) 6701
Bst2UI CCWGG 6 cut(s) 499, 3737, 4236, 5906, 6951, 7055
BstACI GRCGYC 2 cut(s) 78, 6252
BstAPI GCANNNNNTGC 1 cut(s) 3428
BstBAI YACGTR 2 cut(s) 2380, 2950
BstBI TTCGAA 3 cut(s) 59, 156, 298
BstDSI CCRYGG 4 cut(s) 425, 2577, 3147, 4950
BstEII GGTNACC 1 cut(s) 4280
BstENI CCTNNNNNAGG 1 cut(s) 6642
BstFNI CGCG 3 cut(s) 131, 4599, 5075
BstHHI GCGC 3 cut(s) 4599, 6027, 6985
BstNI CCWGG 6 cut(s) 499, 3737, 4236, 5906, 6951, 7055
BstNSI RCATGY 6 cut(s) 154, 704, 1782, 2025, 3403, 4720
BstPAI GACNNNNGTC 1 cut(s) 5725
BstPI GGTNACC 1 cut(s) 4280
BstSFI CTRYAG 9 cut(s) 51, 402, 2519, 3089, 3762, 3861, 5525, 6615, 6986
BstSNI TACGTA 2 cut(s) 2380, 2950
BstUI CGCG 3 cut(s) 131, 4599, 5075
BstV2I GAAGAC 7 cut(s) 887, 2169, 2739, 4577, 5334, 5499, 6860
BstX2I RGATCY 4 cut(s) 4022, 4378, 5149, 7001
BstYI RGATCY 4 cut(s) 4022, 4378, 5149, 7001
BstZ17I GTATAC 1 cut(s) 6701
Bsu15I ATCGAT 2 cut(s) 5466, 6069
BsuI GTATCC 1 cut(s) 3485
BsuRI GGCC 8 cut(s) 24, 497, 3974, 5072, 6123, 6417, 6862, 7065
BsuTUI ATCGAT 2 cut(s) 5466, 6069
BtgI CCRYGG 4 cut(s) 425, 2577, 3147, 4950
BtgZI GCGATG 1 cut(s) 7111
BtrI CACGTC 1 cut(s) 717
BveI ACCTGC 1 cut(s) 5734
CaiI CAGNNNCTG 2 cut(s) 2267, 2837
CciI TCATGA 1 cut(s) 3508
CfoI GCGC 3 cut(s) 4599, 6027, 6985
Cfr10I RCCGGY 1 cut(s) 88
Cfr13I GGNCC 4 cut(s) 2514, 3084, 4263, 6901
ClaI ATCGAT 2 cut(s) 5466, 6069
CseI GACGC 7 cut(s) 137, 1892, 2156, 2726, 5064, 5230, 6241
CspCI CAANNNNNGTGG 8 cut(s) 1753, 1788, 2260, 2295, 2830, 2865, 5049, 5084
DraI TTTAAA 1 cut(s) 4729
DraIII CACNNNGTG 1 cut(s) 4688
DrdI GACNNNNNNGTC 2 cut(s) 3940, 4324
DriI GACNNNNNGTC 2 cut(s) 133, 5701
DseDI GACNNNNNNGTC 2 cut(s) 3940, 4324
EaeI YGGCCR 4 cut(s) 22, 5070, 6860, 7063
Eam1105I GACNNNNNGTC 2 cut(s) 133, 5701
EciI GGCGGA 2 cut(s) 3647, 5198
Ecl136II GAGCTC 4 cut(s) 511, 856, 1806, 4273
Eco105I TACGTA 2 cut(s) 2380, 2950
Eco130I CCWWGG 3 cut(s) 3147, 5847, 6236
Eco24I GRGCYC 4 cut(s) 513, 858, 1808, 4275
Eco31I GGTCTC 5 cut(s) 320, 557, 779, 4395, 4940
Eco32I GATATC 6 cut(s) 175, 5955, 5997, 6067, 6828, 7227
Eco47I GGWCC 4 cut(s) 2514, 3084, 4263, 6901
Eco53kI GAGCTC 4 cut(s) 511, 856, 1806, 4273
Eco91I GGTNACC 1 cut(s) 4280
EcoICRI GAGCTC 4 cut(s) 511, 856, 1806, 4273
EcoNI CCTNNNNNAGG 1 cut(s) 6642
EcoO109I RGGNCCY 4 cut(s) 2514, 3084, 4263, 6901
EcoO65I GGTNACC 1 cut(s) 4280
EcoRI GAATTC 3 cut(s) 300, 1379, 6496
EcoRII CCWGG 6 cut(s) 497, 3735, 4234, 5904, 6949, 7053
EcoRV GATATC 6 cut(s) 175, 5955, 5997, 6067, 6828, 7227
EcoT14I CCWWGG 3 cut(s) 3147, 5847, 6236
EcoT22I ATGCAT 5 cut(s) 604, 2861, 4219, 6148, 6361
EcoT38I GRGCYC 4 cut(s) 513, 858, 1808, 4275
ErhI CCWWGG 3 cut(s) 3147, 5847, 6236
Esp3I CGTCTC 1 cut(s) 5688
FaqI GGGAC 5 cut(s) 2500, 3070, 3296, 5733, 6620
FauI CCCGC 4 cut(s) 124, 1581, 2607, 3177
FauNDI CATATG 1 cut(s) 4465
FbaI TGATCA 2 cut(s) 322, 796
FblI GTMKAC 3 cut(s) 1417, 5078, 6700
FriOI GRGCYC 4 cut(s) 513, 858, 1808, 4275
FspAI RTGCGCAY 1 cut(s) 6026
FspI TGCGCA 1 cut(s) 6026
GlaI GCGC 3 cut(s) 4598, 6026, 6984
GsuI CTGGAG 4 cut(s) 1016, 1734, 3407, 4472
HaeIII GGCC 8 cut(s) 24, 497, 3974, 5072, 6123, 6417, 6862, 7065
HapII CCGG 9 cut(s) 21, 32, 89, 742, 789, 6493, 6678, 6810, 6976
HgaI GACGC 7 cut(s) 137, 1892, 2156, 2726, 5064, 5230, 6241
HhaI GCGC 3 cut(s) 4599, 6027, 6985
Hin1I GRCGYC 2 cut(s) 78, 6252
Hin6I GCGC 3 cut(s) 4597, 6025, 6983
HinP1I GCGC 3 cut(s) 4597, 6025, 6983
HincII GTYRAC 5 cut(s) 659, 1563, 2070, 2640, 5532
HindII GTYRAC 5 cut(s) 659, 1563, 2070, 2640, 5532
HindIII AAGCTT 4 cut(s) 746, 1034, 4082, 5034
HpaI GTTAAC 2 cut(s) 1563, 2640
HpaII CCGG 9 cut(s) 21, 32, 89, 742, 789, 6493, 6678, 6810, 6976
Hpy99I CGWCG 3 cut(s) 80, 83, 173
HpyCH4IV ACGT 7 cut(s) 78, 196, 716, 2009, 2379, 2949, 3978
HpySE526I ACGT 7 cut(s) 78, 196, 716, 2009, 2379, 2949, 3978
Hsp92I GRCGYC 2 cut(s) 78, 6252
HspAI GCGC 3 cut(s) 4597, 6025, 6983
KflI GGGWCCC 2 cut(s) 2514, 3084
Kpn2I TCCGGA 1 cut(s) 31
Ksp22I TGATCA 2 cut(s) 322, 796
KspAI GTTAAC 2 cut(s) 1563, 2640
LguI GCTCTTC 3 cut(s) 5, 1293, 5514
LmnI GCTCC 8 cut(s) 991, 997, 1900, 2347, 2917, 4931, 5913, 6595
MaeII ACGT 7 cut(s) 78, 196, 716, 2009, 2379, 2949, 3978
MbiI CCGCTC 2 cut(s) 2614, 3184
MfeI CAATTG 3 cut(s) 3423, 5082, 6060
MflI RGATCY 4 cut(s) 4022, 4378, 5149, 7001
MhlI GDGCHC 7 cut(s) 513, 858, 1162, 1808, 4275, 4716, 6322
MlsI TGGCCA 1 cut(s) 6862
MluNI TGGCCA 1 cut(s) 6862
Mox20I TGGCCA 1 cut(s) 6862
Mph1103I ATGCAT 5 cut(s) 604, 2861, 4219, 6148, 6361
MroI TCCGGA 1 cut(s) 31
MroXI GAANNNNTTC 9 cut(s) 1302, 2361, 2604, 2623, 2931, 5122, 6040, 6573, 7072
MscI TGGCCA 1 cut(s) 6862
MslI CAYNNNNRTG 1 cut(s) 719
Msp20I TGGCCA 1 cut(s) 6862
MspA1I CMGCKG 5 cut(s) 211, 1580, 2087, 2657, 6614
MspI CCGG 9 cut(s) 21, 32, 89, 742, 789, 6493, 6678, 6810, 6976
MunI CAATTG 3 cut(s) 3423, 5082, 6060
Mva1269I GAATGC 8 cut(s) 67, 970, 1927, 2557, 2859, 3127, 3281, 3318
MvaI CCWGG 6 cut(s) 499, 3737, 4236, 5906, 6951, 7055
MvnI CGCG 3 cut(s) 131, 4599, 5075
NciI CCSGG 4 cut(s) 742, 790, 6679, 6977
NcoI CCATGG 1 cut(s) 3147
NdeI CATATG 1 cut(s) 4465
NheI GCTAGC 1 cut(s) 3740
NlaIV GGNNCC 6 cut(s) 2515, 2516, 3085, 3086, 6043, 7003
NmuCI GTSAC 6 cut(s) 584, 3645, 3942, 4280, 4468, 4611
NsbI TGCGCA 1 cut(s) 6026
NsiI ATGCAT 5 cut(s) 604, 2861, 4219, 6148, 6361
NspI RCATGY 6 cut(s) 154, 704, 1782, 2025, 3403, 4720
NspV TTCGAA 3 cut(s) 59, 156, 298
PagI TCATGA 1 cut(s) 3508
PciI ACATGT 5 cut(s) 150, 700, 1778, 2021, 3399
PciSI GCTCTTC 3 cut(s) 5, 1293, 5514
PctI GAATGC 8 cut(s) 67, 970, 1927, 2557, 2859, 3127, 3281, 3318
PdmI GAANNNNTTC 9 cut(s) 1302, 2361, 2604, 2623, 2931, 5122, 6040, 6573, 7072
PflFI GACNNNGTC 1 cut(s) 891
PfoI TCCNGGA 1 cut(s) 5904
Ppu21I YACGTR 2 cut(s) 2380, 2950
PpuMI RGGWCCY 4 cut(s) 2514, 3084, 4263, 6901
PscI ACATGT 5 cut(s) 150, 700, 1778, 2021, 3399
PshAI GACNNNNGTC 1 cut(s) 5725
Psp124BI GAGCTC 4 cut(s) 513, 858, 1808, 4275
Psp1406I AACGTT 2 cut(s) 196, 3978
Psp5II RGGWCCY 4 cut(s) 2514, 3084, 4263, 6901
Psp6I CCWGG 6 cut(s) 497, 3735, 4234, 5904, 6949, 7053
PspEI GGTNACC 1 cut(s) 4280
PspGI CCWGG 6 cut(s) 497, 3735, 4234, 5904, 6949, 7053
PspN4I GGNNCC 6 cut(s) 2515, 2516, 3085, 3086, 6043, 7003
PspPI GGNCC 4 cut(s) 2514, 3084, 4263, 6901
PspPPI RGGWCCY 4 cut(s) 2514, 3084, 4263, 6901
PsrI GAACNNNNNNTAC 2 cut(s) 3366, 3398
PstI CTGCAG 6 cut(s) 55, 406, 3766, 3865, 6619, 6990
PstNI CAGNNNCTG 2 cut(s) 2267, 2837
PsuI RGATCY 4 cut(s) 4022, 4378, 5149, 7001
PsyI GACNNNGTC 1 cut(s) 891
PvuII CAGCTG 5 cut(s) 211, 1580, 2087, 2657, 6614
RseI CAYNNNNRTG 1 cut(s) 719
SacI GAGCTC 4 cut(s) 513, 858, 1808, 4275
SapI GCTCTTC 3 cut(s) 5, 1293, 5514
Sau96I GGNCC 4 cut(s) 2514, 3084, 4263, 6901
ScaI AGTACT 1 cut(s) 4449
SduI GDGCHC 7 cut(s) 513, 858, 1162, 1808, 4275, 4716, 6322
SfcI CTRYAG 9 cut(s) 51, 402, 2519, 3089, 3762, 3861, 5525, 6615, 6986
SfuI TTCGAA 3 cut(s) 59, 156, 298
SgrAI CRCCGGYG 1 cut(s) 88
SinI GGWCC 4 cut(s) 2514, 3084, 4263, 6901
SmiMI CAYNNNNRTG 1 cut(s) 719
SmlI CTYRAG 7 cut(s) 851, 1121, 1361, 3242, 4802, 4895, 5874
SmoI CTYRAG 7 cut(s) 851, 1121, 1361, 3242, 4802, 4895, 5874
SnaBI TACGTA 2 cut(s) 2380, 2950
SpeI ACTAGT 1 cut(s) 694
SsiI CCGC 9 cut(s) 47, 131, 351, 1588, 2614, 3184, 3632, 5073, 5183
SspI AATATT 4 cut(s) 1552, 2413, 2983, 4501
SstI GAGCTC 4 cut(s) 513, 858, 1808, 4275
StyI CCWWGG 3 cut(s) 3147, 5847, 6236
TaiI ACGT 7 cut(s) 81, 199, 719, 2012, 2382, 2952, 3981
TatI WGTACW 7 cut(s) 697, 1878, 2142, 2712, 3547, 4447, 4823
TauI GCSGC 2 cut(s) 50, 5075
TseFI GTSAC 6 cut(s) 584, 3645, 3942, 4280, 4468, 4611
Tsp45I GTSAC 6 cut(s) 584, 3645, 3942, 4280, 4468, 4611
TspGWI ACGGA 2 cut(s) 2566, 4967
Tth111I GACNNNGTC 1 cut(s) 891
VpaK11BI GGWCC 4 cut(s) 2514, 3084, 4263, 6901
XagI CCTNNNNNAGG 1 cut(s) 6642
XbaI TCTAGA 3 cut(s) 898, 2416, 2986
XceI RCATGY 6 cut(s) 154, 704, 1782, 2025, 3403, 4720
XcmI CCANNNNNNNNNTGG 3 cut(s) 4111, 5594, 6331
XmiI GTMKAC 3 cut(s) 1417, 5078, 6700
XmnI GAANNNNTTC 9 cut(s) 1302, 2361, 2604, 2623, 2931, 5122, 6040, 6573, 7072
ZraI GACGTC 1 cut(s) 79
ZrmI AGTACT 1 cut(s) 4449
Zsp2I ATGCAT 5 cut(s) 604, 2861, 4219, 6148, 6361
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.