Rorug04G0357000

F-box protein At5g07610-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0018189
Physical Location & Seq
Forward (+)
52929244 .. 52930688
1445 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0357000.1

Sequence Viewer

Length: 798 bp
ATGAATCATCAAAACATGTTCTTCTTCCCCCCATTCATAAACCTCTATGTTCTCATTTTCTTTCTCTCATCAGAAACCTCATTTGCTGTGGACTCTCACTACCAAAACTGCAGTGTGCCCAAGTCTTGTGGCGGCCAAAACATAAGCTTTCCGTTCTACATCCCAGGCCAGCAAGAAGCCTACTGCGGCTTTCCCGGGTTTCAACTCAACTGCAGTGGTAATGAAGAAGATGGATACCCTACTCTCCAATTGGCCGGAAATGAGTACTTAGTCCACAATATCAGTTACCGAAACCAAACTCTTCTCGTCTCAAACGCTGCTCTTTCGGACTCACCTGATTGTATTCCATTAGTCCAAAACATAACCTTTCCTACTGATCAGTATGACCTTGTTCCGAACCAAAAAGAAATCGTTTTGCTGTATAATTGCAGCTCTGCTTTGGTTGATGATTCGTTTTCGGAGTACAAATTCGGTAGCTGTGATCCAACGCAGACCAGTAGGACTAGTAGTAGGACTTCAGTTCTGGCGCTGCCTGGGGATAAACACCAACTGTTTGGAAATGTGTCTGACAAGTGTGGAAGAGAAGTGGTGGTAACTCCGGTTGAAGATTATGGCACTAATATTGAGTTGGGTATTGAAGAGGTGTTGAGCAGAGGGTTTATGCTCAAATGGTTTGCAAGCAACTGCAGCCGCTGCGAGGACAGTGGAGGCAAGTGTGGCTTCAGTTCCGAGACGTATCATTTCTGCTGTTTCTGCCCAGATAGGCCTCATGCTGTCAGCTGTAAAGGTAAAGGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box

Protein Analysis

265

Amino Acids

29.41

Weight (kDa)

4.81

Isoelectric Point (pI)

51.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 37 - 104 2.6e-17 Wall-associated receptor kinase galacturonan-binding
WAK_assoc PF14380 166 - 255 4.2e-16 Wall-associated receptor kinase C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0018189)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr4g0444981
rosa_laevigata RLG00000005780
rosa_multiflora Rmu_sc0011561.1_g000009
rosa_roxburghii Rroxscaffold_5G00385490
rosa_rugosa Rorug04G0357000
rosa_samantha Rh4AG418800 Rh4AG418900 Rh4BG430500 Rh4BG430600 Rh4DG426500 Rh5CG312200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 132, 186, 691
AclWI GGATC 1 cut(s) 476
AcoI YGGCCR 2 cut(s) 133, 252
AcsI RAATTY 1 cut(s) 467
AcuI CTGAAG 2 cut(s) 501, 706
AfaI GTAC 2 cut(s) 266, 464
AfiI CCNNNNNNNGG 1 cut(s) 697
AflIII ACRYGT 1 cut(s) 15
AgsI TTSAA 3 cut(s) 203, 605, 638
AhlI ACTAGT 1 cut(s) 503
AjnI CCWGG 2 cut(s) 163, 532
AluBI AGCT 4 cut(s) 147, 432, 477, 780
AluI AGCT 4 cut(s) 147, 432, 477, 780
Alw26I GTCTC 2 cut(s) 313, 725
AlwI GGATC 1 cut(s) 476
AlwNI CAGNNNCTG 1 cut(s) 693
Ama87I CYCGRG 1 cut(s) 194
AoxI GGCC 4 cut(s) 133, 166, 252, 764
ApeKI GCWGC 5 cut(s) 317, 429, 529, 687, 693
ApoI RAATTY 1 cut(s) 467
AspLEI GCGC 1 cut(s) 529
AsuC2I CCSGG 2 cut(s) 195, 196
AsuHPI GGTGA 1 cut(s) 324
AvaI CYCGRG 1 cut(s) 194
BaeGI GKGCMC 1 cut(s) 120
BarI GAAGNNNNNNTAC 2 cut(s) 499, 531
BbvI GCAGC 5 cut(s) 304, 441, 516, 680, 699
BccI CCATC 1 cut(s) 224
BciT130I CCWGG 2 cut(s) 165, 534
BciVI GTATCC 1 cut(s) 227
BclI TGATCA 1 cut(s) 376
BcnI CCSGG 2 cut(s) 195, 196
BcoDI GTCTC 2 cut(s) 313, 725
BcuI ACTAGT 1 cut(s) 503
BfaI CTAG 1 cut(s) 504
BfmI CTRYAG 3 cut(s) 109, 211, 685
BfoI RGCGCY 1 cut(s) 530
BfuI GTATCC 1 cut(s) 227
BisI GCNGC 8 cut(s) 133, 187, 318, 430, 530, 688, 691, 694
BlsI GCNGC 8 cut(s) 134, 188, 319, 431, 531, 689, 692, 695
BmcAI AGTACT 1 cut(s) 266
Bme1390I CCNGG 4 cut(s) 165, 195, 196, 534
BmeT110I CYCGRG 1 cut(s) 194
BmrFI CCNGG 4 cut(s) 165, 195, 196, 534
BpuMI CCSGG 2 cut(s) 195, 196
BsaJI CCNNGG 3 cut(s) 163, 194, 533
BsaWI WCCGGW 1 cut(s) 598
Bsc4I CCNNNNNNNGG 1 cut(s) 697
Bse1I ACTGG 1 cut(s) 495
BseBI CCWGG 2 cut(s) 165, 534
BseDI CCNNGG 3 cut(s) 163, 194, 533
BseGI GGATG 1 cut(s) 159
BseLI CCNNNNNNNGG 1 cut(s) 697
BseNI ACTGG 1 cut(s) 495
BseSI GKGCMC 1 cut(s) 120
BseXI GCAGC 5 cut(s) 304, 441, 516, 680, 699
BshFI GGCC 4 cut(s) 135, 168, 254, 766
BsiHKCI CYCGRG 1 cut(s) 194
BsiSI CCGG 3 cut(s) 195, 255, 599
BslI CCNNNNNNNGG 1 cut(s) 697
BsmAI GTCTC 2 cut(s) 313, 725
BsmBI CGTCTC 2 cut(s) 313, 725
BsnI GGCC 4 cut(s) 135, 168, 254, 766
BsoBI CYCGRG 1 cut(s) 194
Bsp1286I GDGCHC 1 cut(s) 120
Bsp143I GATC 2 cut(s) 376, 481
BspACI CCGC 3 cut(s) 132, 186, 691
BspANI GGCC 4 cut(s) 135, 168, 254, 766
BspMAI CTGCAG 3 cut(s) 113, 215, 689
BspPI GGATC 1 cut(s) 476
BsrI ACTGG 1 cut(s) 495
BssECI CCNNGG 3 cut(s) 163, 194, 533
BssMI GATC 2 cut(s) 376, 481
Bst2UI CCWGG 2 cut(s) 165, 534
Bst4CI ACNGT 2 cut(s) 552, 704
Bst6I CTCTTC 3 cut(s) 306, 574, 633
BstAPI GCANNNNNTGC 1 cut(s) 693
BstC8I GCNNGC 2 cut(s) 170, 679
BstDEI CTNAG 1 cut(s) 268
BstF5I GGATG 1 cut(s) 159
BstH2I RGCGCY 1 cut(s) 530
BstHHI GCGC 1 cut(s) 529
BstKTI GATC 2 cut(s) 379, 484
BstMAI GTCTC 2 cut(s) 313, 725
BstMBI GATC 2 cut(s) 376, 481
BstMWI GCNNNNNNNGC 4 cut(s) 687, 693, 717, 753
BstNI CCWGG 2 cut(s) 165, 534
BstNSI RCATGY 1 cut(s) 19
BstSCI CCNGG 4 cut(s) 163, 193, 194, 532
BstSFI CTRYAG 3 cut(s) 109, 211, 685
BstSLI GKGCMC 1 cut(s) 120
BstV1I GCAGC 5 cut(s) 304, 441, 516, 680, 699
BstXI CCANNNNNNTGG 1 cut(s) 554
BsuI GTATCC 1 cut(s) 227
BsuRI GGCC 4 cut(s) 135, 168, 254, 766
BtsCI GGATG 1 cut(s) 159
BtsI GCAGTG 2 cut(s) 118, 220
BtsIMutI CAGTG 3 cut(s) 118, 220, 709
Cac8I GCNNGC 2 cut(s) 170, 679
CaiI CAGNNNCTG 1 cut(s) 693
CfoI GCGC 1 cut(s) 529
Cfr9I CCCGGG 1 cut(s) 194
Csp6I GTAC 2 cut(s) 265, 463
CspCI CAANNNNNGTGG 4 cut(s) 109, 144, 196, 231
CviAII CATG 2 cut(s) 16, 770
CviQI GTAC 2 cut(s) 265, 463
DdeI CTNAG 1 cut(s) 268
DpnI GATC 2 cut(s) 378, 483
DpnII GATC 2 cut(s) 376, 481
EaeI YGGCCR 2 cut(s) 133, 252
Eam1104I CTCTTC 3 cut(s) 306, 574, 633
EarI CTCTTC 3 cut(s) 306, 574, 633
Eco147I AGGCCT 1 cut(s) 766
Eco57I CTGAAG 2 cut(s) 501, 706
Eco88I CYCGRG 1 cut(s) 194
EcoRII CCWGG 2 cut(s) 163, 532
Esp3I CGTCTC 2 cut(s) 313, 725
FaeI CATG 2 cut(s) 19, 773
FalI AAGNNNNNCTT 2 cut(s) 704, 736
FatI CATG 2 cut(s) 15, 769
FbaI TGATCA 1 cut(s) 376
Fnu4HI GCNGC 8 cut(s) 133, 187, 318, 430, 530, 688, 691, 694
FokI GGATG 1 cut(s) 146
Fsp4HI GCNGC 8 cut(s) 133, 187, 318, 430, 530, 688, 691, 694
FspBI CTAG 1 cut(s) 504
GlaI GCGC 1 cut(s) 528
GluI GCNGC 8 cut(s) 133, 187, 318, 430, 530, 688, 691, 694
HaeII RGCGCY 1 cut(s) 530
HaeIII GGCC 4 cut(s) 135, 168, 254, 766
HapII CCGG 3 cut(s) 195, 255, 599
HhaI GCGC 1 cut(s) 529
Hin1II CATG 2 cut(s) 19, 773
Hin6I GCGC 1 cut(s) 527
HinP1I GCGC 1 cut(s) 527
HindIII AAGCTT 1 cut(s) 145
HinfI GANTC 4 cut(s) 4, 92, 329, 449
HpaII CCGG 3 cut(s) 195, 255, 599
HphI GGTGA 1 cut(s) 324
Hpy166II GTNNAC 2 cut(s) 91, 274
Hpy188I TCNGA 6 cut(s) 73, 328, 396, 460, 568, 730
Hpy8I GTNNAC 2 cut(s) 91, 274
HpyCH4III ACNGT 2 cut(s) 552, 704
HpyCH4IV ACGT 1 cut(s) 734
HpyCH4V TGCA 5 cut(s) 111, 213, 429, 677, 687
HpyF10VI GCNNNNNNNGC 4 cut(s) 687, 693, 717, 753
HpyF3I CTNAG 1 cut(s) 268
HpySE526I ACGT 1 cut(s) 734
Hsp92II CATG 2 cut(s) 19, 773
HspAI GCGC 1 cut(s) 527
Ksp22I TGATCA 1 cut(s) 376
Kzo9I GATC 2 cut(s) 376, 481
Lsp1109I GCAGC 5 cut(s) 304, 441, 516, 680, 699
MaeI CTAG 1 cut(s) 504
MaeII ACGT 1 cut(s) 734
MaeIII GTNAC 2 cut(s) 284, 592
MalI GATC 2 cut(s) 378, 483
MboI GATC 2 cut(s) 376, 481
MboII GAAGA 8 cut(s) 13, 16, 236, 239, 293, 591, 617, 650
MfeI CAATTG 1 cut(s) 248
MhlI GDGCHC 1 cut(s) 120
MluCI AATT 3 cut(s) 248, 424, 467
MlyI GAGTC 2 cut(s) 86, 323
MmeI TCCRAC 1 cut(s) 509
MnlI CCTC 7 cut(s) 53, 88, 634, 647, 691, 701, 777
MspA1I CMGCKG 2 cut(s) 693, 780
MspI CCGG 3 cut(s) 195, 255, 599
MspR9I CCNGG 4 cut(s) 165, 195, 196, 534
MunI CAATTG 1 cut(s) 248
MvaI CCWGG 2 cut(s) 165, 534
MwoI GCNNNNNNNGC 4 cut(s) 687, 693, 717, 753
NciI CCSGG 2 cut(s) 195, 196
NdeII GATC 2 cut(s) 376, 481
NlaIII CATG 2 cut(s) 19, 773
NspI RCATGY 1 cut(s) 19
PceI AGGCCT 1 cut(s) 766
PciI ACATGT 1 cut(s) 15
PfeI GAWTC 2 cut(s) 4, 449
PkrI GCNGC 8 cut(s) 134, 188, 319, 431, 531, 689, 692, 695
PleI GAGTC 2 cut(s) 86, 323
PpsI GAGTC 2 cut(s) 86, 323
PscI ACATGT 1 cut(s) 15
Psp6I CCWGG 2 cut(s) 163, 532
PspGI CCWGG 2 cut(s) 163, 532
PstI CTGCAG 3 cut(s) 113, 215, 689
PstNI CAGNNNCTG 1 cut(s) 693
PvuII CAGCTG 1 cut(s) 780
RsaI GTAC 2 cut(s) 266, 464
RsaNI GTAC 2 cut(s) 265, 463
SatI GCNGC 8 cut(s) 133, 187, 318, 430, 530, 688, 691, 694
Sau3AI GATC 2 cut(s) 376, 481
ScaI AGTACT 1 cut(s) 266
SchI GAGTC 2 cut(s) 86, 323
ScrFI CCNGG 4 cut(s) 165, 195, 196, 534
SduI GDGCHC 1 cut(s) 120
SfcI CTRYAG 3 cut(s) 109, 211, 685
SmaI CCCGGG 1 cut(s) 196
SpeI ACTAGT 1 cut(s) 503
Sse9I AATT 3 cut(s) 248, 424, 467
SseBI AGGCCT 1 cut(s) 766
SsiI CCGC 3 cut(s) 132, 186, 691
SspI AATATT 1 cut(s) 622
SspMI CTAG 1 cut(s) 504
StuI AGGCCT 1 cut(s) 766
StyD4I CCNGG 4 cut(s) 163, 193, 194, 532
TaaI ACNGT 2 cut(s) 552, 704
TaiI ACGT 1 cut(s) 737
TasI AATT 3 cut(s) 248, 424, 467
TatI WGTACW 2 cut(s) 264, 462
TauI GCSGC 3 cut(s) 135, 189, 693
TfiI GAWTC 2 cut(s) 4, 449
TscAI CASTG 3 cut(s) 118, 220, 709
TseI GCWGC 5 cut(s) 317, 429, 529, 687, 693
TspDTI ATGAA 3 cut(s) 17, 25, 237
TspGWI ACGGA 1 cut(s) 141
TspMI CCCGGG 1 cut(s) 194
TspRI CASTG 3 cut(s) 118, 220, 709
XapI RAATTY 1 cut(s) 467
XceI RCATGY 1 cut(s) 19
XmaI CCCGGG 1 cut(s) 194
XspI CTAG 1 cut(s) 504
ZrmI AGTACT 1 cut(s) 266
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.