Rorug04G0387500

NADH-ubiquinone oxidoreductase complex I, 21 kDa subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0011038
Physical Location & Seq
Forward (+)
55646671 .. 55649423
2753 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0387500.1

Sequence Viewer

Length: 1008 bp
ATGTTAACACTCACAGCCAGCTCTCTCCCCATTCGCACCGCTTCCGCAATCCAAAGGGGTCTACTCACTGTTCGCTCCGACGCATTCCCTTCTTTCCTTCCCAAAGAAGTCGAGAACATCAAAGACCCATTTGCCCGGAAACTCGCTACACGAATAGAAAGGCTTCCCGTTAGTTTTGGTGAGAATTGTGTGATGAGTAGCTGTGTGAAGCCGTTGGTACAGAGCGAGACGAGTCCGGTGGTTCTTCTCCATGGTTTTGATAGCTCCTGTTTAGAATGGAGATACACTTATCCATTGCTTGAGGAAGCTGGCCTCGAGACTTGGGCTGTTGACATTCTTGGTTGGGGTTTCTCTGATTTAGAAAGACTCCCCCCATGTAATGTGGCCTCGAAGCGAGACCATTTTTTTCAGCTTTGGAAGTCCTACATTAAAAAGCCAATGATATTGGTTGGACCAAGCCTCGGTGCTGCTGTTGCAATTGACTTTGCAGCCAGCTATCCAGAAGCAGTTGAAAGGCTAGTTTTGATTGATGCAAGTGTGTATACAGAAGGCACCGAAAATCAACCAACCTTGTCTAAAATGTTAGCCTATGCTGGAGCATATATATTGAAGAGTTTCCCTTTGCGCTTCTATGTAACCTTTTTGTGCTTCACTGGCATATCATTGAGTACCAGCCTAGATTGGACAAATATTGGCCGCTTGCATTGCCGGTTTCCTTGGTGGGAGGATGCACTTGTGGATTTCATGATGAGTGGGGTGTACAATGTTAGTGCCCAGATAGAACAGGTAAAGCAGAGGACACTTATCATATGGGGCGAGGAAGACCAGATCATTAGCAACAAGCTTGGAGTGAGATTGCACTCTGAACTGCCAGATGCAATTATACGACAAATACCAGATTGCGGACACATTCCTCATGTAGAAAGGCCGAGCTCTGTTGCGAAAATGATTCTGGAATTTGTTCGAGAAGCTCAGTACAAAGAGGTTGAATATAGTACTCAATTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NADH-u_ox-rdase

Protein Analysis

335

Amino Acids

37.84

Weight (kDa)

5.62

Isoelectric Point (pI)

43.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Abhydrolase_1 PF00561 79 - 209 2.2e-10 alpha/beta hydrolase fold
Abhydrolase_6 PF12697 80 - 314 2.5e-15 Alpha/beta hydrolase family
Hydrolase_4 PF12146 80 - 183 7.2e-10 Serine aminopeptidase, S33
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011038)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G17230 AT4G17230
fragaria_vesca FvH4_3g00670 FvH4_3g00670 FvH4_3g00670
malus_domestica MD05G1360300.v1.1 MD10G1336100.v1.1
prunus_persica Prupe.4G007500_v2.0.a1
pyrus_communis pycom05g32540 pycom10g28560
rosa_chinensis RchiOBHm_Chr5g0000891
rosa_laevigata RLG00000030904
rosa_roxburghii Rroxscaffold_1G00075360
rosa_rugosa Rorug04G0387500
rosa_samantha Rh5AG007200 Rh5BG009300 Rh5CG007900 Rh5DG007800
rosa_wichuraiana Rw0G008760 Rw5G000750

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 551
AccI GTMKAC 2 cut(s) 61, 542
AciI CCGC 4 cut(s) 39, 45, 697, 903
AcoI YGGCCR 1 cut(s) 694
AcsI RAATTY 1 cut(s) 956
AfaI GTAC 5 cut(s) 219, 670, 761, 977, 997
AfiI CCNNNNNNNGG 2 cut(s) 461, 902
AgsI TTSAA 3 cut(s) 512, 610, 989
AluBI AGCT 9 cut(s) 21, 201, 264, 308, 412, 495, 844, 933, 971
AluI AGCT 9 cut(s) 21, 201, 264, 308, 412, 495, 844, 933, 971
Alw21I GWGCWC 1 cut(s) 935
Alw26I GTCTC 3 cut(s) 221, 311, 390
Ama87I CYCGRG 1 cut(s) 314
AoxI GGCC 4 cut(s) 310, 384, 694, 926
ApeKI GCWGC 2 cut(s) 467, 488
ApoI RAATTY 1 cut(s) 956
Asp700I GAANNNNTTC 3 cut(s) 162, 614, 960
AspLEI GCGC 1 cut(s) 627
AspS9I GGNCC 1 cut(s) 452
AsuC2I CCSGG 1 cut(s) 136
AsuHPI GGTGA 1 cut(s) 191
AvaI CYCGRG 1 cut(s) 314
AvaII GGWCC 1 cut(s) 452
BaeGI GKGCMC 1 cut(s) 775
BanI GGYRCC 1 cut(s) 551
BanII GRGCYC 1 cut(s) 935
BbsI GAAGAC 1 cut(s) 828
Bbv12I GWGCWC 1 cut(s) 935
BbvI GCAGC 2 cut(s) 454, 500
BceAI ACGGC 1 cut(s) 196
BcnI CCSGG 1 cut(s) 136
BcoDI GTCTC 3 cut(s) 221, 311, 390
BfaI CTAG 2 cut(s) 518, 677
BisI GCNGC 3 cut(s) 468, 489, 697
BlsI GCNGC 3 cut(s) 469, 490, 698
BmcAI AGTACT 1 cut(s) 997
Bme1390I CCNGG 1 cut(s) 136
Bme18I GGWCC 1 cut(s) 452
BmeT110I CYCGRG 1 cut(s) 314
BmgT120I GGNCC 1 cut(s) 452
BmiI GGNNCC 1 cut(s) 553
BmrFI CCNGG 1 cut(s) 136
BmsI GCATC 3 cut(s) 520, 718, 865
BpiI GAAGAC 1 cut(s) 828
BpmI CTGGAG 1 cut(s) 615
BpuEI CTTGAG 1 cut(s) 320
BpuMI CCSGG 1 cut(s) 136
BsaI GGTCTC 1 cut(s) 390
BsaJI CCNNGG 3 cut(s) 250, 460, 716
BsaWI WCCGGW 1 cut(s) 235
Bsc4I CCNNNNNNNGG 2 cut(s) 461, 902
Bse118I RCCGGY 1 cut(s) 708
Bse1I ACTGG 1 cut(s) 658
Bse3DI GCAATG 2 cut(s) 293, 703
BseDI CCNNGG 3 cut(s) 250, 460, 716
BseGI GGATG 1 cut(s) 733
BseLI CCNNNNNNNGG 2 cut(s) 461, 902
BseMI GCAATG 2 cut(s) 293, 703
BseMII CTCAG 1 cut(s) 986
BseNI ACTGG 1 cut(s) 658
BseSI GKGCMC 1 cut(s) 775
BseXI GCAGC 2 cut(s) 454, 500
BshFI GGCC 4 cut(s) 312, 386, 696, 928
BshNI GGYRCC 1 cut(s) 551
BsiHKAI GWGCWC 1 cut(s) 935
BsiHKCI CYCGRG 1 cut(s) 314
BsiSI CCGG 3 cut(s) 136, 236, 709
BslI CCNNNNNNNGG 2 cut(s) 461, 902
BsmAI GTCTC 3 cut(s) 221, 311, 390
BsmBI CGTCTC 1 cut(s) 221
BsmI GAATGC 1 cut(s) 83
BsnI GGCC 4 cut(s) 312, 386, 696, 928
Bso31I GGTCTC 1 cut(s) 390
BsoBI CYCGRG 1 cut(s) 314
Bsp1286I GDGCHC 2 cut(s) 775, 935
Bsp1407I TGTACA 1 cut(s) 759
Bsp143I GATC 1 cut(s) 828
Bsp19I CCATGG 1 cut(s) 250
BspACI CCGC 4 cut(s) 39, 45, 697, 903
BspANI GGCC 4 cut(s) 312, 386, 696, 928
BspCNI CTCAG 1 cut(s) 985
BspHI TCATGA 1 cut(s) 744
BspLI GGNNCC 1 cut(s) 553
BspT107I GGYRCC 1 cut(s) 551
BspTNI GGTCTC 1 cut(s) 390
BsrDI GCAATG 2 cut(s) 293, 703
BsrFI RCCGGY 1 cut(s) 708
BsrGI TGTACA 1 cut(s) 759
BsrI ACTGG 1 cut(s) 658
BssAI RCCGGY 1 cut(s) 708
BssECI CCNNGG 3 cut(s) 250, 460, 716
BssMI GATC 1 cut(s) 828
BssNAI GTATAC 1 cut(s) 543
BssT1I CCWWGG 2 cut(s) 250, 716
Bst1107I GTATAC 1 cut(s) 543
Bst4CI ACNGT 1 cut(s) 70
Bst6I CTCTTC 1 cut(s) 605
BstAUI TGTACA 1 cut(s) 759
BstC8I GCNNGC 4 cut(s) 19, 310, 493, 701
BstDEI CTNAG 1 cut(s) 972
BstDSI CCRYGG 1 cut(s) 250
BstF5I GGATG 1 cut(s) 733
BstHHI GCGC 1 cut(s) 627
BstKTI GATC 1 cut(s) 831
BstMAI GTCTC 3 cut(s) 221, 311, 390
BstMBI GATC 1 cut(s) 828
BstMWI GCNNNNNNNGC 3 cut(s) 473, 654, 705
BstSCI CCNGG 1 cut(s) 134
BstSLI GKGCMC 1 cut(s) 775
BstV1I GCAGC 2 cut(s) 454, 500
BstV2I GAAGAC 1 cut(s) 828
BstZ17I GTATAC 1 cut(s) 543
BsuRI GGCC 4 cut(s) 312, 386, 696, 928
BtgI CCRYGG 1 cut(s) 250
BtsCI GGATG 1 cut(s) 733
BtsIMutI CAGTG 2 cut(s) 66, 651
Cac8I GCNNGC 4 cut(s) 19, 310, 493, 701
CciI TCATGA 1 cut(s) 744
CfoI GCGC 1 cut(s) 627
Cfr10I RCCGGY 1 cut(s) 708
Cfr13I GGNCC 1 cut(s) 452
CseI GACGC 1 cut(s) 89
Csp6I GTAC 5 cut(s) 218, 669, 760, 976, 996
CviAII CATG 4 cut(s) 251, 375, 745, 917
CviQI GTAC 5 cut(s) 218, 669, 760, 976, 996
DdeI CTNAG 1 cut(s) 972
DpnI GATC 1 cut(s) 830
DpnII GATC 1 cut(s) 828
EaeI YGGCCR 1 cut(s) 694
Eam1104I CTCTTC 1 cut(s) 605
EarI CTCTTC 1 cut(s) 605
Ecl136II GAGCTC 1 cut(s) 933
Eco130I CCWWGG 2 cut(s) 250, 716
Eco24I GRGCYC 1 cut(s) 935
Eco31I GGTCTC 1 cut(s) 390
Eco47I GGWCC 1 cut(s) 452
Eco53kI GAGCTC 1 cut(s) 933
Eco88I CYCGRG 1 cut(s) 314
EcoICRI GAGCTC 1 cut(s) 933
EcoT14I CCWWGG 2 cut(s) 250, 716
EcoT38I GRGCYC 1 cut(s) 935
ErhI CCWWGG 2 cut(s) 250, 716
Esp3I CGTCTC 1 cut(s) 221
FaeI CATG 4 cut(s) 254, 378, 748, 920
FatI CATG 4 cut(s) 250, 374, 744, 916
FauNDI CATATG 1 cut(s) 809
FblI GTMKAC 2 cut(s) 61, 542
Fnu4HI GCNGC 3 cut(s) 468, 489, 697
FokI GGATG 1 cut(s) 740
FriOI GRGCYC 1 cut(s) 935
Fsp4HI GCNGC 3 cut(s) 468, 489, 697
FspBI CTAG 2 cut(s) 518, 677
GlaI GCGC 1 cut(s) 626
GluI GCNGC 3 cut(s) 468, 489, 697
GsuI CTGGAG 1 cut(s) 615
HaeIII GGCC 4 cut(s) 312, 386, 696, 928
HapII CCGG 3 cut(s) 136, 236, 709
HgaI GACGC 1 cut(s) 89
HhaI GCGC 1 cut(s) 627
Hin1II CATG 4 cut(s) 254, 378, 748, 920
Hin6I GCGC 1 cut(s) 625
HinP1I GCGC 1 cut(s) 625
HincII GTYRAC 2 cut(s) 6, 331
HindII GTYRAC 2 cut(s) 6, 331
HindIII AAGCTT 1 cut(s) 842
HinfI GANTC 3 cut(s) 232, 366, 949
HpaI GTTAAC 1 cut(s) 6
HpaII CCGG 3 cut(s) 136, 236, 709
HphI GGTGA 1 cut(s) 191
Hpy166II GTNNAC 5 cut(s) 6, 62, 331, 543, 760
Hpy188I TCNGA 4 cut(s) 79, 355, 865, 1007
Hpy188III TCNNGA 6 cut(s) 112, 316, 500, 745, 953, 965
Hpy8I GTNNAC 5 cut(s) 6, 62, 331, 543, 760
Hpy99I CGWCG 1 cut(s) 83
HpyAV CCTTC 3 cut(s) 99, 107, 542
HpyCH4III ACNGT 1 cut(s) 70
HpyCH4V TGCA 7 cut(s) 476, 488, 533, 703, 731, 859, 878
HpyF10VI GCNNNNNNNGC 3 cut(s) 473, 654, 705
HpyF3I CTNAG 1 cut(s) 972
Hsp92II CATG 4 cut(s) 254, 378, 748, 920
HspAI GCGC 1 cut(s) 625
KspAI GTTAAC 1 cut(s) 6
Kzo9I GATC 1 cut(s) 828
LmnI GCTCC 3 cut(s) 80, 269, 596
Lsp1109I GCAGC 2 cut(s) 454, 500
LweI GCATC 3 cut(s) 520, 718, 865
MaeI CTAG 2 cut(s) 518, 677
MaeIII GTNAC 1 cut(s) 634
MalI GATC 1 cut(s) 830
MboI GATC 1 cut(s) 828
MboII GAAGA 3 cut(s) 236, 622, 833
MfeI CAATTG 1 cut(s) 477
MhlI GDGCHC 2 cut(s) 775, 935
MluCI AATT 5 cut(s) 184, 477, 879, 956, 1001
MlyI GAGTC 2 cut(s) 241, 360
MmeI TCCRAC 2 cut(s) 102, 430
MnlI CCTC 9 cut(s) 295, 323, 397, 470, 718, 789, 811, 924, 976
MroXI GAANNNNTTC 3 cut(s) 162, 614, 960
MseI TTAA 2 cut(s) 5, 429
MspI CCGG 3 cut(s) 136, 236, 709
MspR9I CCNGG 1 cut(s) 136
MunI CAATTG 1 cut(s) 477
Mva1269I GAATGC 1 cut(s) 83
MwoI GCNNNNNNNGC 3 cut(s) 473, 654, 705
NciI CCSGG 1 cut(s) 136
NcoI CCATGG 1 cut(s) 250
NdeI CATATG 1 cut(s) 809
NdeII GATC 1 cut(s) 828
NlaIII CATG 4 cut(s) 254, 378, 748, 920
NlaIV GGNNCC 1 cut(s) 553
NmeAIII GCCGAG 1 cut(s) 954
PaeR7I CTCGAG 1 cut(s) 314
PagI TCATGA 1 cut(s) 744
PctI GAATGC 1 cut(s) 83
PdmI GAANNNNTTC 3 cut(s) 162, 614, 960
PfeI GAWTC 1 cut(s) 949
PkrI GCNGC 3 cut(s) 469, 490, 698
PleI GAGTC 2 cut(s) 240, 360
PpsI GAGTC 2 cut(s) 240, 360
Psp124BI GAGCTC 1 cut(s) 935
PspN4I GGNNCC 1 cut(s) 553
PspPI GGNCC 1 cut(s) 452
RsaI GTAC 5 cut(s) 219, 670, 761, 977, 997
RsaNI GTAC 5 cut(s) 218, 669, 760, 976, 996
SacI GAGCTC 1 cut(s) 935
SaqAI TTAA 2 cut(s) 5, 429
SatI GCNGC 3 cut(s) 468, 489, 697
Sau3AI GATC 1 cut(s) 828
Sau96I GGNCC 1 cut(s) 452
ScaI AGTACT 1 cut(s) 997
SchI GAGTC 2 cut(s) 241, 360
ScrFI CCNGG 1 cut(s) 136
SduI GDGCHC 2 cut(s) 775, 935
SfaNI GCATC 3 cut(s) 520, 718, 865
Sfr274I CTCGAG 1 cut(s) 314
SinI GGWCC 1 cut(s) 452
SlaI CTCGAG 1 cut(s) 314
SmlI CTYRAG 2 cut(s) 299, 314
SmoI CTYRAG 2 cut(s) 299, 314
Sse9I AATT 5 cut(s) 184, 477, 879, 956, 1001
SsiI CCGC 4 cut(s) 39, 45, 697, 903
SspI AATATT 1 cut(s) 691
SspMI CTAG 2 cut(s) 518, 677
SstI GAGCTC 1 cut(s) 935
StyD4I CCNGG 1 cut(s) 134
StyI CCWWGG 2 cut(s) 250, 716
TaaI ACNGT 1 cut(s) 70
TaqI TCGA 4 cut(s) 111, 315, 389, 964
TasI AATT 5 cut(s) 184, 477, 879, 956, 1001
TatI WGTACW 3 cut(s) 759, 975, 995
TauI GCSGC 1 cut(s) 699
TfiI GAWTC 1 cut(s) 949
Tru1I TTAA 2 cut(s) 5, 429
Tru9I TTAA 2 cut(s) 5, 429
TscAI CASTG 2 cut(s) 73, 658
TseI GCWGC 2 cut(s) 467, 488
TspDTI ATGAA 1 cut(s) 733
TspRI CASTG 2 cut(s) 73, 658
VpaK11BI GGWCC 1 cut(s) 452
XapI RAATTY 1 cut(s) 956
XhoI CTCGAG 1 cut(s) 314
XmiI GTMKAC 2 cut(s) 61, 542
XmnI GAANNNNTTC 3 cut(s) 162, 614, 960
XspI CTAG 2 cut(s) 518, 677
ZrmI AGTACT 1 cut(s) 997
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.