Rorug05G0014300

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0017959
Physical Location & Seq
Forward (+)
949156 .. 950071
916 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0014300.1

Sequence Viewer

Length: 480 bp
ATGACAAGCCTATATAAGAAGCACACAGTACCTCAGGTTTTTTGCATCCTAAATCAAAACCAATATCAACTTACCATTCCATTGACTCCTCTGTTCCAATTCCAAAAAAGTGCTAGTATGGAGAAGAAAATGATGACTTTCGCTGGGTTGGTGATTATGGTCGTCGTCCTCAATGCATGTCCTGCAATTGGAGGGTGCGACGAAGCCTTAGTCGAGTTGTTGCCCTGTGCCACGTTCGTGTTGGGCCCTGGCATAGGGGAGCCCCCTTCTCCGTGCTGTGCAGGTTTAAAGCATGTGGTTGCTGGAGCTGATACCAGTGAGAAGCGCAGGAGTCTTTGCCAATGTTTGAAAGACAAAGCTGTTGGTGCTGGCGTTAAAGTCGACCCTGAAAAACTTAAGCACGTTACTGACGCTTGTGGCGTCAAAATTCCTATTCCTATTGATCCCAATGTCGACTGCAGCACAATTCCGGTGTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

159

Amino Acids

17.0

Weight (kDa)

8.03

Isoelectric Point (pI)

31.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LTP_2 PF14368 57 - 144 7.1e-08 Probable lipid transfer
Tryp_alpha_amyl PF00234 66 - 153 4e-11 Protease inhibitor/seed storage/LTP family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017959)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G18335
fragaria_vesca FvH4_3g05631
prunus_persica Prupe.4G080200_v2.0.a1
rosa_laevigata RLG00000032062
rosa_multiflora Rmu_sc0005803.1_g000010 Rmu_sc0008587.1_g000001
rosa_roxburghii Rroxscaffold_1G00062700
rosa_rugosa Rorug05G0014200 Rorug05G0014300
rosa_samantha Rh5DG104100
rosa_wichuraiana Rw5G009480

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 272
AccI GTMKAC 2 cut(s) 381, 453
AclWI GGATC 1 cut(s) 437
AcsI RAATTY 1 cut(s) 426
AcyI GRCGYC 1 cut(s) 420
AfaI GTAC 1 cut(s) 30
AfiI CCNNNNNNNGG 2 cut(s) 188, 254
AflII CTTAAG 1 cut(s) 395
AgsI TTSAA 1 cut(s) 349
AjnI CCWGG 1 cut(s) 247
AleI CACNNNNGTG 1 cut(s) 236
AluBI AGCT 2 cut(s) 308, 359
AluI AGCT 2 cut(s) 308, 359
AlwI GGATC 1 cut(s) 437
AoxI GGCC 1 cut(s) 244
ApaI GGGCCC 1 cut(s) 248
ApeKI GCWGC 1 cut(s) 459
ApoI RAATTY 1 cut(s) 426
ArsI GACNNNNNNTTYG 2 cut(s) 195, 227
AspLEI GCGC 1 cut(s) 327
AspS9I GGNCC 2 cut(s) 244, 245
AsuHPI GGTGA 1 cut(s) 163
AxyI CCTNAGG 1 cut(s) 33
BaeGI GKGCMC 1 cut(s) 248
BanII GRGCYC 2 cut(s) 248, 264
BbvI GCAGC 1 cut(s) 471
BciT130I CCWGG 1 cut(s) 249
BfaI CTAG 1 cut(s) 114
BfmI CTRYAG 1 cut(s) 457
BfrI CTTAAG 1 cut(s) 395
BfuAI ACCTGC 1 cut(s) 272
BisI GCNGC 1 cut(s) 460
BlsI GCNGC 1 cut(s) 461
Bme1390I CCNGG 1 cut(s) 249
BmgT120I GGNCC 2 cut(s) 244, 245
BmiI GGNNCC 2 cut(s) 246, 261
BmrFI CCNGG 1 cut(s) 249
BmsI GCATC 1 cut(s) 54
BpmI CTGGAG 1 cut(s) 324
BsaHI GRCGYC 1 cut(s) 420
BsaJI CCNNGG 1 cut(s) 247
BsaWI WCCGGW 1 cut(s) 469
Bsc4I CCNNNNNNNGG 2 cut(s) 188, 254
Bse1I ACTGG 1 cut(s) 315
Bse21I CCTNAGG 1 cut(s) 33
BseBI CCWGG 1 cut(s) 249
BseDI CCNNGG 1 cut(s) 247
BseGI GGATG 1 cut(s) 45
BseLI CCNNNNNNNGG 2 cut(s) 188, 254
BseMII CTCAG 1 cut(s) 47
BseNI ACTGG 1 cut(s) 315
BseRI GAGGAG 1 cut(s) 78
BseSI GKGCMC 1 cut(s) 248
BseXI GCAGC 1 cut(s) 471
BseYI CCCAGC 1 cut(s) 143
BsgI GTGCAG 1 cut(s) 300
BshFI GGCC 1 cut(s) 246
BsiSI CCGG 1 cut(s) 470
BslI CCNNNNNNNGG 2 cut(s) 188, 254
BsnI GGCC 1 cut(s) 246
Bsp120I GGGCCC 1 cut(s) 244
Bsp1286I GDGCHC 2 cut(s) 248, 264
Bsp143I GATC 1 cut(s) 442
BspANI GGCC 1 cut(s) 246
BspCNI CTCAG 1 cut(s) 46
BspLI GGNNCC 2 cut(s) 246, 261
BspMAI CTGCAG 1 cut(s) 461
BspMI ACCTGC 1 cut(s) 272
BspPI GGATC 1 cut(s) 437
BspTI CTTAAG 1 cut(s) 395
BsrI ACTGG 1 cut(s) 315
BssECI CCNNGG 1 cut(s) 247
BssMI GATC 1 cut(s) 442
BssNI GRCGYC 1 cut(s) 420
Bst2UI CCWGG 1 cut(s) 249
Bst4CI ACNGT 1 cut(s) 28
BstACI GRCGYC 1 cut(s) 420
BstAFI CTTAAG 1 cut(s) 395
BstAPI GCANNNNNTGC 1 cut(s) 182
BstC8I GCNNGC 1 cut(s) 370
BstDEI CTNAG 2 cut(s) 33, 208
BstENI CCTNNNNNAGG 1 cut(s) 252
BstF5I GGATG 1 cut(s) 45
BstHHI GCGC 1 cut(s) 327
BstKTI GATC 1 cut(s) 445
BstMBI GATC 1 cut(s) 442
BstMWI GCNNNNNNNGC 2 cut(s) 182, 365
BstNI CCWGG 1 cut(s) 249
BstNSI RCATGY 2 cut(s) 180, 296
BstSCI CCNGG 1 cut(s) 247
BstSFI CTRYAG 1 cut(s) 457
BstSLI GKGCMC 1 cut(s) 248
BstV1I GCAGC 1 cut(s) 471
Bsu36I CCTNAGG 1 cut(s) 33
BsuRI GGCC 1 cut(s) 246
BtsCI GGATG 1 cut(s) 45
BtsIMutI CAGTG 1 cut(s) 322
BveI ACCTGC 1 cut(s) 272
Cac8I GCNNGC 1 cut(s) 370
CfoI GCGC 1 cut(s) 327
Cfr13I GGNCC 2 cut(s) 244, 245
CseI GACGC 2 cut(s) 409, 419
Csp6I GTAC 1 cut(s) 29
CviAII CATG 2 cut(s) 177, 293
CviJI RGCY 6 cut(s) 9, 206, 246, 262, 308, 359
CviKI_1 RGCY 6 cut(s) 9, 206, 246, 262, 308, 359
CviQI GTAC 1 cut(s) 29
DdeI CTNAG 2 cut(s) 33, 208
DpnI GATC 1 cut(s) 444
DpnII GATC 1 cut(s) 442
DraI TTTAAA 1 cut(s) 288
Eco24I GRGCYC 2 cut(s) 248, 264
Eco81I CCTNAGG 1 cut(s) 33
EcoNI CCTNNNNNAGG 1 cut(s) 252
EcoO109I RGGNCCY 1 cut(s) 245
EcoRII CCWGG 1 cut(s) 247
EcoT22I ATGCAT 1 cut(s) 178
EcoT38I GRGCYC 2 cut(s) 248, 264
FaeI CATG 2 cut(s) 180, 296
FaiI YATR 7 cut(s) 13, 15, 119, 158, 178, 254, 294
FatI CATG 2 cut(s) 176, 292
FblI GTMKAC 2 cut(s) 381, 453
Fnu4HI GCNGC 1 cut(s) 460
FokI GGATG 1 cut(s) 32
FriOI GRGCYC 2 cut(s) 248, 264
Fsp4HI GCNGC 1 cut(s) 460
FspBI CTAG 1 cut(s) 114
GlaI GCGC 1 cut(s) 326
GluI GCNGC 1 cut(s) 460
GsaI CCCAGC 1 cut(s) 147
GsuI CTGGAG 1 cut(s) 324
HaeIII GGCC 1 cut(s) 246
HapII CCGG 1 cut(s) 470
HgaI GACGC 2 cut(s) 409, 419
HhaI GCGC 1 cut(s) 327
Hin1I GRCGYC 1 cut(s) 420
Hin1II CATG 2 cut(s) 180, 296
Hin6I GCGC 1 cut(s) 325
HinP1I GCGC 1 cut(s) 325
HincII GTYRAC 2 cut(s) 382, 454
HindII GTYRAC 2 cut(s) 382, 454
HinfI GANTC 2 cut(s) 85, 331
HpaII CCGG 1 cut(s) 470
HphI GGTGA 1 cut(s) 163
Hpy166II GTNNAC 2 cut(s) 382, 454
Hpy8I GTNNAC 2 cut(s) 382, 454
Hpy99I CGWCG 2 cut(s) 167, 203
HpyAV CCTTC 1 cut(s) 276
HpyCH4III ACNGT 1 cut(s) 28
HpyCH4IV ACGT 2 cut(s) 233, 402
HpyCH4V TGCA 5 cut(s) 45, 176, 185, 281, 459
HpyF10VI GCNNNNNNNGC 2 cut(s) 182, 365
HpyF3I CTNAG 2 cut(s) 33, 208
HpySE526I ACGT 2 cut(s) 233, 402
Hsp92I GRCGYC 1 cut(s) 420
Hsp92II CATG 2 cut(s) 180, 296
HspAI GCGC 1 cut(s) 325
Kzo9I GATC 1 cut(s) 442
LmnI GCTCC 2 cut(s) 259, 305
Lsp1109I GCAGC 1 cut(s) 471
LweI GCATC 1 cut(s) 54
MaeI CTAG 1 cut(s) 114
MaeII ACGT 2 cut(s) 233, 402
MaeIII GTNAC 1 cut(s) 403
MalI GATC 1 cut(s) 444
MboI GATC 1 cut(s) 442
MboII GAAGA 1 cut(s) 136
MfeI CAATTG 1 cut(s) 186
MhlI GDGCHC 2 cut(s) 248, 264
MluCI AATT 4 cut(s) 98, 186, 426, 465
MlyI GAGTC 2 cut(s) 79, 340
MnlI CCTC 4 cut(s) 42, 99, 179, 185
Mph1103I ATGCAT 1 cut(s) 178
MseI TTAA 4 cut(s) 287, 375, 396, 478
MslI CAYNNNNRTG 1 cut(s) 236
MspCI CTTAAG 1 cut(s) 395
MspI CCGG 1 cut(s) 470
MspR9I CCNGG 1 cut(s) 249
MunI CAATTG 1 cut(s) 186
MvaI CCWGG 1 cut(s) 249
MwoI GCNNNNNNNGC 2 cut(s) 182, 365
NdeII GATC 1 cut(s) 442
NlaIII CATG 2 cut(s) 180, 296
NlaIV GGNNCC 2 cut(s) 246, 261
NsiI ATGCAT 1 cut(s) 178
NspI RCATGY 2 cut(s) 180, 296
OliI CACNNNNGTG 1 cut(s) 236
PcsI WCGNNNNNNNCGW 1 cut(s) 417
PkrI GCNGC 1 cut(s) 461
PleI GAGTC 2 cut(s) 79, 339
PpsI GAGTC 2 cut(s) 79, 339
Psp6I CCWGG 1 cut(s) 247
PspFI CCCAGC 1 cut(s) 143
PspGI CCWGG 1 cut(s) 247
PspN4I GGNNCC 2 cut(s) 246, 261
PspOMI GGGCCC 1 cut(s) 244
PspPI GGNCC 2 cut(s) 244, 245
PstI CTGCAG 1 cut(s) 461
RsaI GTAC 1 cut(s) 30
RsaNI GTAC 1 cut(s) 29
RseI CAYNNNNRTG 1 cut(s) 236
SalI GTCGAC 2 cut(s) 380, 452
SaqAI TTAA 4 cut(s) 287, 375, 396, 478
SatI GCNGC 1 cut(s) 460
Sau3AI GATC 1 cut(s) 442
Sau96I GGNCC 2 cut(s) 244, 245
SchI GAGTC 2 cut(s) 79, 340
ScrFI CCNGG 1 cut(s) 249
SduI GDGCHC 2 cut(s) 248, 264
SetI ASST 7 cut(s) 34, 39, 236, 286, 310, 361, 405
SfaNI GCATC 1 cut(s) 54
SfcI CTRYAG 1 cut(s) 457
SmiMI CAYNNNNRTG 1 cut(s) 236
SmlI CTYRAG 1 cut(s) 395
SmoI CTYRAG 1 cut(s) 395
Sse9I AATT 4 cut(s) 98, 186, 426, 465
SspMI CTAG 1 cut(s) 114
StyD4I CCNGG 1 cut(s) 247
TaaI ACNGT 1 cut(s) 28
TaiI ACGT 2 cut(s) 236, 405
TaqI TCGA 3 cut(s) 213, 381, 453
TasI AATT 4 cut(s) 98, 186, 426, 465
Tru1I TTAA 4 cut(s) 287, 375, 396, 478
Tru9I TTAA 4 cut(s) 287, 375, 396, 478
TscAI CASTG 1 cut(s) 322
TseI GCWGC 1 cut(s) 459
TspGWI ACGGA 1 cut(s) 261
TspRI CASTG 1 cut(s) 322
Vha464I CTTAAG 1 cut(s) 395
XagI CCTNNNNNAGG 1 cut(s) 252
XapI RAATTY 1 cut(s) 426
XceI RCATGY 2 cut(s) 180, 296
XcmI CCANNNNNNNNNTGG 1 cut(s) 238
XmiI GTMKAC 2 cut(s) 381, 453
XspI CTAG 1 cut(s) 114
Zsp2I ATGCAT 1 cut(s) 178
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.