Rorug05G0344200

Protein of unknown function, DUF604

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0014929
Physical Location & Seq
Forward (+)
42349378 .. 42352642
3265 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0344200.1

Sequence Viewer

Length: 2205 bp
ATGATGTGTGTATCATCTTTGACTTTGATACTGACAATTCATGTCTCAAAATGCAGTGGCATGCGGTCATTACAGGTTTCCAAGACACGGCTCTTCTCCTCACTGCCATCTATTACTCCCCTCTCTGATGTAGAAACCCAATTGAGAGCTTTTTGGAAGGACCCGGATCCAAAAATTTCGGAGGCGCTTTCGCTTTTTCATCATGCCATACATTCCAACCGCCTGCCTCCGGGGTCAGCCTCAGCCTGCAACTTCCTTGTAGACACTCTCGCAAGGTCAAAAAATTATGAACTATCATTCTCAGTTTATACCAAGATGACCAAGGTTGGTATTATTCCAAGCTTTATATCATTGAGTTGTTTAGTTTCATATTTTGTCAATACGCGTAAGCTAGAATTCGCACCCGGGGTTTTCGGCTTGGTATTAAAGCGTGGATTTCAGTTAAATGAGTATGTTATGAATCTTGCATTAAACGGTTTTTGTTCTAATGATGAGGTTGACAAGGCAATTGAGTTGTTTAGTGTAATGAGAAGACATTTTGTAAAGCCTGGTATTCGTAGTTATAACATTCTTATAAATGGACTTTGCAAAGCGAAAAAGTTGAAAGAAGCAGTGGAATTGTTGGTTGATATGGAGGTGTCGGATTCTGAACCGAATGTGGTAACATACAGCACATTGATTAATGGATTTTGTAAACAGGGTAGAGTGGACGAGGCCATGGGTTTCTTGGAGGAGATGAGGCAAAAGGGTTTGGAAATGGATGTTTTTGTATATGGGGCCCTTATAAGTGGTTTTTGTGCTAAAGGGAGTTTTGATAGAGGAAAACAACTTTTTGATGAGATGTTAAACAAGGGTATTTCTCCTAATGTGGTTACGTATAGTTGTTTGATTAATTATCTTTCAAAGACAGGTAAGTGGAAAGAAGTCACTGTGATGGTAAATGATATGACACAATGTGGAGTTCCCCCTGATGCTGTTACTTACACAGGTATCCTTGATGGGCTTTTCAAAAATGGAATGGCTACAAAAGCCATGGCGATATTCAATTTAATGCTATTGAAGGGTACAGAGCCTAATAATGTAACATACAACGTTATGATTGATGGACTATGCAAGGAGGGGTTGGTGGATGATGCTTTAAAGATCTTAGAAATGATGAAAGGGAAAGGTGTAAGGCCTGATGTAATCACTTACAACACATTACTAATGGGACTATCCTGTGATGGGAAGGTTGATGAGATGATGCAACTTTTCAGTAAGATAACAAATGATGAGAACTTTGTTGAGCCAGATGTTATGACATATAACATGCTCATTTTGGGACTGTGCAAGGAAGGTAATCTTGATCAGGCTATGGAGATGTATCATACAATGGTTGAAAGGGGCATTTCTGGTAGCTTATGTACTTATAACACTTTGATCGACAGATGCATACAGGAAGGATTAGTTGACAAGGCCATAGAATTCTGGAGACATGCAGTTAACTTGGGAATTATACCTAACTCAATCACTTATGGTGTCTTGATCAATGGATTTTGCAAAAATCAAATGGTCAGGCTTGCGAAAGGACTTTTTAGTAAAATGAGAGCTTCTGGGATTATTCCTACTGTAATTGACTACAACACTTTGATGTCATCGTTGTGCAAGGAGGGTAGTTTGGAGCAAGCTAGGATGTTGTTTCAAGAAATGAGAACTGCAAATCATTCGCCTTGTGTTGTCTCATTTAATACCATAATTGACGGACTTCTTAAAGCTGGAGATATTAAATCTACTAAAGAGTTGCTAGAGGATATGTTCAAATTGGGTTTAATTCCCGATAGAATTACTTTCTCTACATTAGTAAACCGGTTTTCAAAACTTGGGCTATTGGCTGAGGCTAGAATGTGTGTTGAGAAGATGATTGCTCGTGGTATTGAACCTGATGTCTTTGTATTCGATTCTTTATTAAAGGGCTACAGTTCTAAAGGTGAAACAGAAGAAATTGTTAATTTGCTTCATCAGATGGCAGAAAAGGGTGTTGTTCTTGACAAAGATATAACTTCTACAATCTTGGCGTGCCTCTGTCAAATCTCTGATGACGTTGATGTGATGGAGGTTCTCCCTACTTTTTCCAAAGAGACATCGAAAGGAATAAGCATTACCTGCAATGAGCTATTGATGAAAGTCAATAAATTTTATCCGGAACTTAAGTTATGTGCTGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF604

Protein Analysis

734

Amino Acids

81.65

Weight (kDa)

6.23

Isoelectric Point (pI)

22.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPR_24 PF23276 132 - 241 5.2e-07 Fungal tetratrico peptide repeats
PPR_2 PF13041 149 - 197 6.3e-12 PPR repeat family
PPR_1 PF12854 181 - 212 5.3e-10 PPR repeat
PPR_2 PF13041 183 - 232 4.5e-17 PPR repeat family
PPR PF01535 187 - 213 1.3e-06 PPR repeat
PPR_1 PF12854 217 - 247 2.7e-16 PPR repeat
PPR_2 PF13041 218 - 266 8.3e-17 PPR repeat family
PPR PF01535 221 - 251 2.8e-10 PPR repeat
PPR_3 PF13812 243 - 300 1e-10 Pentatricopeptide repeat domain
PPR_1 PF12854 249 - 281 3.5e-08 PPR repeat
PPR_2 PF13041 254 - 298 1.2e-13 PPR repeat family
PPR PF01535 257 - 286 8e-06 PPR repeat
PPR_long PF17177 270 - 348 1.1e-06 Pentacotripeptide-repeat region of PRORP
PPR_2 PF13041 288 - 337 2.3e-14 PPR repeat family
PPR_3 PF13812 312 - 368 1.3e-08 Pentatricopeptide repeat domain
PPR_1 PF12854 320 - 351 3.4e-06 PPR repeat
PPR_2 PF13041 326 - 372 1.2e-14 PPR repeat family
TPR_24 PF23276 338 - 457 3.6e-09 Fungal tetratrico peptide repeats
PPR_1 PF12854 354 - 387 4e-13 PPR repeat
PPR_2 PF13041 361 - 405 4.1e-16 PPR repeat family
PPR PF01535 361 - 391 3.3e-10 PPR repeat
PPR_3 PF13812 381 - 439 2.9e-08 Pentatricopeptide repeat domain
PPR_1 PF12854 389 - 420 1e-07 PPR repeat
PPR_3 PF13812 428 - 477 8.7e-09 Pentatricopeptide repeat domain
PPR_1 PF12854 428 - 459 4.4e-11 PPR repeat
PPR_2 PF13041 430 - 477 2.3e-14 PPR repeat family
PPR PF01535 434 - 463 4.4e-07 PPR repeat
PPR_long PF17177 443 - 565 7.2e-07 Pentacotripeptide-repeat region of PRORP
PPR_2 PF13041 468 - 514 1.1e-12 PPR repeat family
PPR_3 PF13812 492 - 546 6.2e-07 Pentatricopeptide repeat domain
PPR_1 PF12854 497 - 529 2.9e-08 PPR repeat
PPR_2 PF13041 500 - 537 2.4e-07 PPR repeat family
PPR PF01535 503 - 533 3.9e-06 PPR repeat
PPR_1 PF12854 532 - 564 6e-09 PPR repeat
PPR_2 PF13041 535 - 570 6.9e-07 PPR repeat family
PPR PF01535 540 - 566 2.4e-06 PPR repeat
PPR_2 PF13041 570 - 619 4.7e-12 PPR repeat family
PPR_2 PF13041 640 - 688 7.9e-06 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0014929)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G07850
fragaria_vesca FvH4_3g34260
malus_domestica MD03G1107200.v1.1 MD11G1119800.v1.1
prunus_persica Prupe.6G089900_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0061811
rosa_laevigata RLG00000035508
rosa_multiflora Rmu_sc0004134.1_g000009
rosa_roxburghii Rroxscaffold_1G00018920
rosa_rugosa Rorug05G0344100 Rorug05G0344200
rosa_samantha Rh5AG405000 Rh5BG418000 Rh5CG442500 Rh5DG432100
rosa_wichuraiana Rw5G038040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 4 cut(s) 564, 575, 785, 1410
Acc36I ACCTGC 1 cut(s) 2150
AccI GTMKAC 1 cut(s) 261
AccII CGCG 1 cut(s) 385
AccIII TCCGGA 1 cut(s) 2179
AciI CCGC 2 cut(s) 64, 220
AclI AACGTT 1 cut(s) 1092
AclWI GGATC 2 cut(s) 161, 174
AcsI RAATTY 4 cut(s) 174, 395, 1463, 2171
AdeI CACNNNGTG 1 cut(s) 956
AfaI GTAC 2 cut(s) 1066, 1405
AfiI CCNNNNNNNGG 3 cut(s) 87, 229, 1224
AflII CTTAAG 1 cut(s) 2186
AflIII ACRYGT 1 cut(s) 383
AgeI ACCGGT 1 cut(s) 1845
AjnI CCWGG 1 cut(s) 547
AluBI AGCT 8 cut(s) 149, 342, 391, 1398, 1589, 1667, 1754, 2152
AluI AGCT 8 cut(s) 149, 342, 391, 1398, 1589, 1667, 1754, 2152
Alw26I GTCTC 4 cut(s) 49, 1465, 1723, 2111
AlwI GGATC 2 cut(s) 161, 174
Ama87I CYCGRG 1 cut(s) 404
Aor13HI TCCGGA 1 cut(s) 2179
AoxI GGCC 4 cut(s) 714, 777, 1175, 1455
ApaI GGGCCC 1 cut(s) 781
ApeKI GCWGC 1 cut(s) 2198
ApoI RAATTY 4 cut(s) 174, 395, 1463, 2171
AseI ATTAAT 2 cut(s) 681, 891
AsiGI ACCGGT 1 cut(s) 1845
AspLEI GCGC 1 cut(s) 187
AspS9I GGNCC 3 cut(s) 160, 777, 778
AsuC2I CCSGG 4 cut(s) 164, 231, 405, 406
AsuHPI GGTGA 1 cut(s) 1979
AvaI CYCGRG 1 cut(s) 404
AvaII GGWCC 1 cut(s) 160
BaeGI GKGCMC 1 cut(s) 781
BaeI ACNNNNGTAYC 2 cut(s) 973, 1006
BamHI GGATCC 1 cut(s) 166
BanII GRGCYC 1 cut(s) 781
BauI CACGAG 1 cut(s) 1905
BbsI GAAGAC 1 cut(s) 538
BbvCI CCTCAGC 2 cut(s) 241, 1872
BbvI GCAGC 1 cut(s) 2185
BccI CCATC 7 cut(s) 115, 928, 992, 1097, 1217, 1996, 2083
BceAI ACGGC 1 cut(s) 104
BcgI CGANNNNNNTGC 2 cut(s) 1686, 1720
BciT130I CCWGG 1 cut(s) 549
BciVI GTATCC 1 cut(s) 1001
BclI TGATCA 2 cut(s) 1345, 1524
BcnI CCSGG 4 cut(s) 164, 231, 405, 406
BcoDI GTCTC 4 cut(s) 49, 1465, 1723, 2111
BfaI CTAG 4 cut(s) 392, 1668, 1784, 1878
BfmI CTRYAG 1 cut(s) 1954
BfoI RGCGCY 1 cut(s) 188
BfrI CTTAAG 1 cut(s) 2186
BfuAI ACCTGC 1 cut(s) 2150
BfuI GTATCC 1 cut(s) 1001
BglII AGATCT 1 cut(s) 1143
BisI GCNGC 1 cut(s) 2199
BlsI GCNGC 1 cut(s) 2200
Bme1390I CCNGG 5 cut(s) 164, 231, 405, 406, 549
Bme18I GGWCC 1 cut(s) 160
BmeT110I CYCGRG 1 cut(s) 404
BmgT120I GGNCC 3 cut(s) 160, 777, 778
BmiI GGNNCC 4 cut(s) 162, 168, 778, 779
BmrFI CCNGG 5 cut(s) 164, 231, 405, 406, 549
BmsI GCATC 4 cut(s) 961, 1123, 1233, 1418
BpiI GAAGAC 1 cut(s) 538
BpmI CTGGAG 2 cut(s) 1489, 1776
Bpu10I CCTNAGC 2 cut(s) 241, 1872
BpuMI CCSGG 4 cut(s) 164, 231, 405, 406
BsaAI YACGTR 1 cut(s) 876
BsaJI CCNNGG 6 cut(s) 230, 321, 404, 405, 717, 1032
BsaWI WCCGGW 2 cut(s) 1845, 2179
Bsc4I CCNNNNNNNGG 3 cut(s) 87, 229, 1224
Bse118I RCCGGY 1 cut(s) 1845
Bse3DI GCAATG 1 cut(s) 2152
BseAI TCCGGA 1 cut(s) 2179
BseBI CCWGG 1 cut(s) 549
BseDI CCNNGG 6 cut(s) 230, 321, 404, 405, 717, 1032
BseGI GGATG 3 cut(s) 766, 1135, 1677
BseLI CCNNNNNNNGG 3 cut(s) 87, 229, 1224
BseMI GCAATG 1 cut(s) 2152
BseMII CTCAG 3 cut(s) 255, 315, 1863
BseRI GAGGAG 2 cut(s) 88, 746
BseSI GKGCMC 1 cut(s) 781
BseXI GCAGC 1 cut(s) 2185
Bsh1236I CGCG 1 cut(s) 385
BshFI GGCC 4 cut(s) 716, 779, 1177, 1457
BshTI ACCGGT 1 cut(s) 1845
BsiHKCI CYCGRG 1 cut(s) 404
BsiSI CCGG 5 cut(s) 164, 230, 405, 1846, 2180
BslFI GGGAC 2 cut(s) 1224, 1335
BslI CCNNNNNNNGG 3 cut(s) 87, 229, 1224
BsmAI GTCTC 4 cut(s) 49, 1465, 1723, 2111
BsmFI GGGAC 2 cut(s) 1224, 1335
BsnI GGCC 4 cut(s) 716, 779, 1177, 1457
BsoBI CYCGRG 1 cut(s) 404
Bsp120I GGGCCC 1 cut(s) 777
Bsp1286I GDGCHC 1 cut(s) 781
Bsp13I TCCGGA 1 cut(s) 2179
Bsp143I GATC 5 cut(s) 166, 1143, 1345, 1419, 1524
Bsp19I CCATGG 2 cut(s) 717, 1032
BspACI CCGC 2 cut(s) 64, 220
BspANI GGCC 4 cut(s) 716, 779, 1177, 1457
BspCNI CTCAG 3 cut(s) 254, 314, 1864
BspEI TCCGGA 1 cut(s) 2179
BspFNI CGCG 1 cut(s) 385
BspLI GGNNCC 4 cut(s) 162, 168, 778, 779
BspMI ACCTGC 1 cut(s) 2150
BspPI GGATC 2 cut(s) 161, 174
BspQI GCTCTTC 1 cut(s) 98
BspTI CTTAAG 1 cut(s) 2186
BsrDI GCAATG 1 cut(s) 2152
BsrFI RCCGGY 1 cut(s) 1845
BssAI RCCGGY 1 cut(s) 1845
BssECI CCNNGG 6 cut(s) 230, 321, 404, 405, 717, 1032
BssMI GATC 5 cut(s) 166, 1143, 1345, 1419, 1524
BssSI CACGAG 1 cut(s) 1905
BssT1I CCWWGG 3 cut(s) 321, 717, 1032
Bst2BI CACGAG 1 cut(s) 1905
Bst2UI CCWGG 1 cut(s) 549
Bst4CI ACNGT 5 cut(s) 476, 931, 1326, 1609, 1958
Bst6I CTCTTC 1 cut(s) 98
BstAFI CTTAAG 1 cut(s) 2186
BstAPI GCANNNNNTGC 1 cut(s) 2142
BstBAI YACGTR 1 cut(s) 876
BstC8I GCNNGC 6 cut(s) 62, 224, 247, 1560, 1665, 2056
BstDEI CTNAG 4 cut(s) 241, 301, 1147, 1872
BstDSI CCRYGG 2 cut(s) 717, 1032
BstF5I GGATG 3 cut(s) 766, 1135, 1677
BstFNI CGCG 1 cut(s) 385
BstH2I RGCGCY 1 cut(s) 188
BstHHI GCGC 1 cut(s) 187
BstKTI GATC 5 cut(s) 169, 1146, 1348, 1422, 1527
BstMAI GTCTC 4 cut(s) 49, 1465, 1723, 2111
BstMBI GATC 5 cut(s) 166, 1143, 1345, 1419, 1524
BstMWI GCNNNNNNNGC 2 cut(s) 1028, 2142
BstNI CCWGG 1 cut(s) 549
BstNSI RCATGY 3 cut(s) 64, 1312, 1478
BstSCI CCNGG 5 cut(s) 162, 229, 403, 404, 547
BstSFI CTRYAG 1 cut(s) 1954
BstSLI GKGCMC 1 cut(s) 781
BstSNI TACGTA 1 cut(s) 876
BstUI CGCG 1 cut(s) 385
BstV1I GCAGC 1 cut(s) 2185
BstV2I GAAGAC 1 cut(s) 538
BstX2I RGATCY 2 cut(s) 166, 1143
BstYI RGATCY 2 cut(s) 166, 1143
BsuI GTATCC 1 cut(s) 1001
BsuRI GGCC 4 cut(s) 716, 779, 1177, 1457
BtgI CCRYGG 2 cut(s) 717, 1032
BtsCI GGATG 3 cut(s) 766, 1135, 1677
BtsI GCAGTG 3 cut(s) 61, 101, 618
BtsIMutI CAGTG 4 cut(s) 61, 101, 618, 927
BveI ACCTGC 1 cut(s) 2150
Cac8I GCNNGC 6 cut(s) 62, 224, 247, 1560, 1665, 2056
CfoI GCGC 1 cut(s) 187
Cfr10I RCCGGY 1 cut(s) 1845
Cfr13I GGNCC 3 cut(s) 160, 777, 778
Cfr9I CCCGGG 1 cut(s) 404
Csp6I GTAC 2 cut(s) 1065, 1404
CspAI ACCGGT 1 cut(s) 1845
CviAII CATG 7 cut(s) 41, 61, 203, 718, 1033, 1309, 1475
CviQI GTAC 2 cut(s) 1065, 1404
DdeI CTNAG 4 cut(s) 241, 301, 1147, 1872
DpnI GATC 5 cut(s) 168, 1145, 1347, 1421, 1526
DpnII GATC 5 cut(s) 166, 1143, 1345, 1419, 1524
DraI TTTAAA 1 cut(s) 1140
DraIII CACNNNGTG 1 cut(s) 956
Eam1104I CTCTTC 1 cut(s) 98
EarI CTCTTC 1 cut(s) 98
Eco105I TACGTA 1 cut(s) 876
Eco130I CCWWGG 3 cut(s) 321, 717, 1032
Eco147I AGGCCT 1 cut(s) 1177
Eco24I GRGCYC 1 cut(s) 781
Eco47I GGWCC 1 cut(s) 160
Eco88I CYCGRG 1 cut(s) 404
EcoO109I RGGNCCY 3 cut(s) 160, 777, 778
EcoRI GAATTC 2 cut(s) 395, 1463
EcoRII CCWGG 1 cut(s) 547
EcoT14I CCWWGG 3 cut(s) 321, 717, 1032
EcoT22I ATGCAT 1 cut(s) 1433
EcoT38I GRGCYC 1 cut(s) 781
ErhI CCWWGG 3 cut(s) 321, 717, 1032
FaeI CATG 7 cut(s) 44, 64, 206, 721, 1036, 1312, 1478
FalI AAGNNNNNCTT 2 cut(s) 1326, 1358
FaqI GGGAC 2 cut(s) 1224, 1335
FatI CATG 7 cut(s) 40, 60, 202, 717, 1032, 1308, 1474
FbaI TGATCA 2 cut(s) 1345, 1524
FblI GTMKAC 1 cut(s) 261
Fnu4HI GCNGC 1 cut(s) 2199
FokI GGATG 3 cut(s) 773, 1142, 1684
FriOI GRGCYC 1 cut(s) 781
Fsp4HI GCNGC 1 cut(s) 2199
FspBI CTAG 4 cut(s) 392, 1668, 1784, 1878
GlaI GCGC 1 cut(s) 186
GluI GCNGC 1 cut(s) 2199
GsuI CTGGAG 2 cut(s) 1489, 1776
HaeII RGCGCY 1 cut(s) 188
HaeIII GGCC 4 cut(s) 716, 779, 1177, 1457
HapII CCGG 5 cut(s) 164, 230, 405, 1846, 2180
HhaI GCGC 1 cut(s) 187
Hin1II CATG 7 cut(s) 44, 64, 206, 721, 1036, 1312, 1478
Hin6I GCGC 1 cut(s) 185
HinP1I GCGC 1 cut(s) 185
HincII GTYRAC 3 cut(s) 499, 1450, 1483
HindII GTYRAC 3 cut(s) 499, 1450, 1483
HindIII AAGCTT 1 cut(s) 340
HinfI GANTC 3 cut(s) 460, 644, 1937
HpaI GTTAAC 1 cut(s) 1483
HpaII CCGG 5 cut(s) 164, 230, 405, 1846, 2180
HphI GGTGA 1 cut(s) 1979
Hpy166II GTNNAC 7 cut(s) 262, 499, 695, 709, 1450, 1483, 1843
Hpy188I TCNGA 6 cut(s) 127, 181, 643, 649, 2001, 2074
Hpy188III TCNNGA 7 cut(s) 1343, 1468, 1522, 1682, 1814, 2024, 2180
Hpy8I GTNNAC 7 cut(s) 262, 499, 695, 709, 1450, 1483, 1843
HpyAV CCTTC 5 cut(s) 151, 1054, 1222, 1328, 1433
HpyCH4III ACNGT 5 cut(s) 476, 931, 1326, 1609, 1958
HpyCH4IV ACGT 3 cut(s) 875, 1092, 2079
HpyF10VI GCNNNNNNNGC 2 cut(s) 1028, 2142
HpyF3I CTNAG 4 cut(s) 241, 301, 1147, 1872
HpySE526I ACGT 3 cut(s) 875, 1092, 2079
Hsp92II CATG 7 cut(s) 44, 64, 206, 721, 1036, 1312, 1478
HspAI GCGC 1 cut(s) 185
Kpn2I TCCGGA 1 cut(s) 2179
Ksp22I TGATCA 2 cut(s) 1345, 1524
KspAI GTTAAC 1 cut(s) 1483
Kzo9I GATC 5 cut(s) 166, 1143, 1345, 1419, 1524
LguI GCTCTTC 1 cut(s) 98
LmnI GCTCC 1 cut(s) 1660
Lsp1109I GCAGC 1 cut(s) 2185
LweI GCATC 4 cut(s) 961, 1123, 1233, 1418
MaeI CTAG 4 cut(s) 392, 1668, 1784, 1878
MaeII ACGT 3 cut(s) 875, 1092, 2079
MaeIII GTNAC 5 cut(s) 661, 871, 925, 976, 1081
MalI GATC 5 cut(s) 168, 1145, 1347, 1421, 1526
MboI GATC 5 cut(s) 166, 1143, 1345, 1419, 1524
MboII GAAGA 4 cut(s) 85, 543, 1906, 1988
MfeI CAATTG 2 cut(s) 140, 507
MflI RGATCY 2 cut(s) 166, 1143
MhlI GDGCHC 1 cut(s) 781
MluI ACGCGT 1 cut(s) 383
MmeI TCCRAC 2 cut(s) 240, 621
Mph1103I ATGCAT 1 cut(s) 1433
MroI TCCGGA 1 cut(s) 2179
MslI CAYNNNNRTG 3 cut(s) 932, 1628, 1639
MspCI CTTAAG 1 cut(s) 2186
MspI CCGG 5 cut(s) 164, 230, 405, 1846, 2180
MspR9I CCNGG 5 cut(s) 164, 231, 405, 406, 549
MunI CAATTG 2 cut(s) 140, 507
MvaI CCWGG 1 cut(s) 549
MvnI CGCG 1 cut(s) 385
MwoI GCNNNNNNNGC 2 cut(s) 1028, 2142
NciI CCSGG 4 cut(s) 164, 231, 405, 406
NcoI CCATGG 2 cut(s) 717, 1032
NdeII GATC 5 cut(s) 166, 1143, 1345, 1419, 1524
NlaIII CATG 7 cut(s) 44, 64, 206, 721, 1036, 1312, 1478
NlaIV GGNNCC 4 cut(s) 162, 168, 778, 779
NmuCI GTSAC 1 cut(s) 925
NsiI ATGCAT 1 cut(s) 1433
NspI RCATGY 3 cut(s) 64, 1312, 1478
PaeI GCATGC 1 cut(s) 64
PceI AGGCCT 1 cut(s) 1177
PciSI GCTCTTC 1 cut(s) 98
PfeI GAWTC 3 cut(s) 460, 644, 1937
PinAI ACCGGT 1 cut(s) 1845
PkrI GCNGC 1 cut(s) 2200
Ppu21I YACGTR 1 cut(s) 876
PpuMI RGGWCCY 1 cut(s) 160
PshBI ATTAAT 2 cut(s) 681, 891
PsiI TTATAA 4 cut(s) 564, 575, 785, 1410
Psp1406I AACGTT 1 cut(s) 1092
Psp5II RGGWCCY 1 cut(s) 160
Psp6I CCWGG 1 cut(s) 547
PspGI CCWGG 1 cut(s) 547
PspN4I GGNNCC 4 cut(s) 162, 168, 778, 779
PspOMI GGGCCC 1 cut(s) 777
PspPI GGNCC 3 cut(s) 160, 777, 778
PspPPI RGGWCCY 1 cut(s) 160
PsuI RGATCY 2 cut(s) 166, 1143
RsaI GTAC 2 cut(s) 1066, 1405
RsaNI GTAC 2 cut(s) 1065, 1404
RseI CAYNNNNRTG 3 cut(s) 932, 1628, 1639
SapI GCTCTTC 1 cut(s) 98
SatI GCNGC 1 cut(s) 2199
Sau3AI GATC 5 cut(s) 166, 1143, 1345, 1419, 1524
Sau96I GGNCC 3 cut(s) 160, 777, 778
ScrFI CCNGG 5 cut(s) 164, 231, 405, 406, 549
SduI GDGCHC 1 cut(s) 781
SfaNI GCATC 4 cut(s) 961, 1123, 1233, 1418
SfcI CTRYAG 1 cut(s) 1954
SinI GGWCC 1 cut(s) 160
SmaI CCCGGG 1 cut(s) 406
SmiMI CAYNNNNRTG 3 cut(s) 932, 1628, 1639
SmlI CTYRAG 1 cut(s) 2186
SmoI CTYRAG 1 cut(s) 2186
SnaBI TACGTA 1 cut(s) 876
SphI GCATGC 1 cut(s) 64
SseBI AGGCCT 1 cut(s) 1177
SsiI CCGC 2 cut(s) 64, 220
SspMI CTAG 4 cut(s) 392, 1668, 1784, 1878
StuI AGGCCT 1 cut(s) 1177
StyD4I CCNGG 5 cut(s) 162, 229, 403, 404, 547
StyI CCWWGG 3 cut(s) 321, 717, 1032
TaaI ACNGT 5 cut(s) 476, 931, 1326, 1609, 1958
TaiI ACGT 3 cut(s) 878, 1095, 2082
TaqI TCGA 3 cut(s) 1422, 1935, 2123
TatI WGTACW 1 cut(s) 1403
TfiI GAWTC 3 cut(s) 460, 644, 1937
TscAI CASTG 4 cut(s) 61, 108, 618, 934
TseFI GTSAC 1 cut(s) 925
TseI GCWGC 1 cut(s) 2198
Tsp45I GTSAC 1 cut(s) 925
TspDTI ATGAA 8 cut(s) 29, 188, 303, 357, 473, 1172, 1985, 2174
TspGWI ACGGA 1 cut(s) 1755
TspMI CCCGGG 1 cut(s) 404
TspRI CASTG 4 cut(s) 61, 108, 618, 934
Vha464I CTTAAG 1 cut(s) 2186
VpaK11BI GGWCC 1 cut(s) 160
VspI ATTAAT 2 cut(s) 681, 891
XapI RAATTY 4 cut(s) 174, 395, 1463, 2171
XceI RCATGY 3 cut(s) 64, 1312, 1478
XcmI CCANNNNNNNNNTGG 1 cut(s) 724
XmaI CCCGGG 1 cut(s) 404
XmiI GTMKAC 1 cut(s) 261
XspI CTAG 4 cut(s) 392, 1668, 1784, 1878
Zsp2I ATGCAT 1 cut(s) 1433
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.