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| Species | Orthologous Gene IDs |
|---|---|
| arabidopsis_thaliana | AT5G50175 |
| prunus_persica | Prupe.8G004000_v2.0.a1 |
| rosa_chinensis | RchiOBHm_Chr6g0249651 |
| rosa_multiflora | Rmu_co8264641.1_g000001 Rmu_sc0000704.1_g000046 Rmu_sc0004691.1_g000002 |
| rosa_rugosa | Rorug05G0494300 |
| rosa_samantha | Rh6AG004400 Rh6BG005700 Rh6CG003400 |
| rosa_wichuraiana | Rw6G000480 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 2 cut(s) | 573, 2316 |
| AasI | GACNNNNNNGTC | 1 cut(s) | 2573 |
| Acc36I | ACCTGC | 1 cut(s) | 217 |
| AccI | GTMKAC | 1 cut(s) | 638 |
| AccII | CGCG | 1 cut(s) | 1633 |
| AccIII | TCCGGA | 1 cut(s) | 952 |
| AciI | CCGC | 5 cut(s) | 836, 1393, 1631, 1813, 3368 |
| AclWI | GGATC | 4 cut(s) | 573, 633, 944, 957 |
| AcoI | YGGCCR | 2 cut(s) | 1155, 1353 |
| AcuI | CTGAAG | 2 cut(s) | 1486, 1598 |
| AcvI | CACGTG | 1 cut(s) | 650 |
| AfaI | GTAC | 4 cut(s) | 2935, 2973, 3145, 3151 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 2596 |
| AflII | CTTAAG | 1 cut(s) | 2183 |
| AhlI | ACTAGT | 1 cut(s) | 2117 |
| AjiI | CACGTC | 1 cut(s) | 716 |
| AjnI | CCWGG | 6 cut(s) | 879, 964, 1524, 2045, 2335, 3304 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 2160, 2192 |
| Alw21I | GWGCWC | 5 cut(s) | 31, 721, 964, 2557, 2865 |
| Alw26I | GTCTC | 3 cut(s) | 1524, 1603, 2846 |
| Alw44I | GTGCAC | 3 cut(s) | 960, 2553, 2861 |
| AlwI | GGATC | 4 cut(s) | 573, 633, 944, 957 |
| Aor13HI | TCCGGA | 1 cut(s) | 952 |
| AoxI | GGCC | 8 cut(s) | 310, 1155, 1353, 1705, 2048, 2333, 3209, 3435 |
| ApaLI | GTGCAC | 3 cut(s) | 960, 2553, 2861 |
| ApeKI | GCWGC | 8 cut(s) | 234, 237, 405, 497, 1086, 3290, 3406, 3569 |
| AseI | ATTAAT | 2 cut(s) | 2546, 3132 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 2494 |
| AspA2I | CCTAGG | 1 cut(s) | 2735 |
| AspLEI | GCGC | 2 cut(s) | 831, 2679 |
| AspS9I | GGNCC | 4 cut(s) | 311, 968, 1019, 2926 |
| AsuC2I | CCSGG | 1 cut(s) | 2195 |
| AsuHPI | GGTGA | 4 cut(s) | 17, 382, 683, 2801 |
| AsuII | TTCGAA | 4 cut(s) | 854, 1053, 2261, 2485 |
| AvaII | GGWCC | 3 cut(s) | 968, 1019, 2926 |
| AvrII | CCTAGG | 1 cut(s) | 2735 |
| BaeGI | GKGCMC | 3 cut(s) | 964, 2557, 2865 |
| BalI | TGGCCA | 2 cut(s) | 1157, 1355 |
| BamHI | GGATCC | 1 cut(s) | 949 |
| BanII | GRGCYC | 1 cut(s) | 31 |
| BauI | CACGAG | 2 cut(s) | 510, 3294 |
| BbrPI | CACGTG | 1 cut(s) | 650 |
| BbsI | GAAGAC | 1 cut(s) | 378 |
| Bbv12I | GWGCWC | 5 cut(s) | 31, 721, 964, 2557, 2865 |
| BbvI | GCAGC | 8 cut(s) | 224, 246, 392, 484, 1098, 3302, 3393, 3556 |
| BccI | CCATC | 8 cut(s) | 662, 818, 919, 968, 2336, 2594, 2633, 3278 |
| BceAI | ACGGC | 1 cut(s) | 1342 |
| BciT130I | CCWGG | 6 cut(s) | 881, 966, 1526, 2047, 2337, 3306 |
| BciVI | GTATCC | 1 cut(s) | 832 |
| BclI | TGATCA | 2 cut(s) | 265, 1128 |
| BcnI | CCSGG | 1 cut(s) | 2195 |
| BcoDI | GTCTC | 3 cut(s) | 1524, 1603, 2846 |
| BcuI | ACTAGT | 1 cut(s) | 2117 |
| BfaI | CTAG | 8 cut(s) | 932, 1559, 1650, 2118, 2724, 2736, 3051, 3628 |
| BfmI | CTRYAG | 3 cut(s) | 2466, 2475, 2856 |
| BfoI | RGCGCY | 1 cut(s) | 832 |
| BfrI | CTTAAG | 1 cut(s) | 2183 |
| BfuAI | ACCTGC | 1 cut(s) | 217 |
| BfuI | GTATCC | 1 cut(s) | 832 |
| BisI | GCNGC | 8 cut(s) | 235, 238, 406, 498, 1087, 3291, 3407, 3570 |
| BlnI | CCTAGG | 1 cut(s) | 2735 |
| BlpI | GCTNAGC | 2 cut(s) | 832, 3402 |
| BlsI | GCNGC | 8 cut(s) | 236, 239, 407, 499, 1088, 3292, 3408, 3571 |
| BmcAI | AGTACT | 1 cut(s) | 2935 |
| Bme1390I | CCNGG | 7 cut(s) | 881, 966, 1526, 2047, 2195, 2337, 3306 |
| Bme18I | GGWCC | 3 cut(s) | 968, 1019, 2926 |
| BmgBI | CACGTC | 1 cut(s) | 716 |
| BmgT120I | GGNCC | 4 cut(s) | 311, 968, 1019, 2926 |
| BmiI | GGNNCC | 1 cut(s) | 951 |
| BmrFI | CCNGG | 7 cut(s) | 881, 966, 1526, 2047, 2195, 2337, 3306 |
| BmsI | GCATC | 6 cut(s) | 358, 753, 1230, 2425, 3105, 3196 |
| BpiI | GAAGAC | 1 cut(s) | 378 |
| BpmI | CTGGAG | 1 cut(s) | 902 |
| Bpu10I | CCTNAGC | 1 cut(s) | 705 |
| Bpu1102I | GCTNAGC | 2 cut(s) | 832, 3402 |
| Bpu14I | TTCGAA | 4 cut(s) | 854, 1053, 2261, 2485 |
| BpuEI | CTTGAG | 2 cut(s) | 440, 542 |
| BpuMI | CCSGG | 1 cut(s) | 2195 |
| BsaAI | YACGTR | 1 cut(s) | 650 |
| BsaBI | GATNNNNATC | 2 cut(s) | 270, 465 |
| BsaI | GGTCTC | 1 cut(s) | 1524 |
| BsaJI | CCNNGG | 8 cut(s) | 209, 642, 879, 1525, 2253, 2699, 2735, 3524 |
| BsaWI | WCCGGW | 2 cut(s) | 952, 2968 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 2936, 2966 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 2596 |
| Bse118I | RCCGGY | 1 cut(s) | 1489 |
| Bse1I | ACTGG | 2 cut(s) | 688, 1350 |
| Bse3DI | GCAATG | 2 cut(s) | 93, 1958 |
| Bse8I | GATNNNNATC | 2 cut(s) | 270, 465 |
| BseAI | TCCGGA | 1 cut(s) | 952 |
| BseBI | CCWGG | 6 cut(s) | 881, 966, 1526, 2047, 2337, 3306 |
| BseDI | CCNNGG | 8 cut(s) | 209, 642, 879, 1525, 2253, 2699, 2735, 3524 |
| BseJI | GATNNNNATC | 2 cut(s) | 270, 465 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 2596 |
| BseMI | GCAATG | 2 cut(s) | 93, 1958 |
| BseMII | CTCAG | 7 cut(s) | 846, 1281, 1793, 2066, 2757, 3005, 3393 |
| BseNI | ACTGG | 2 cut(s) | 688, 1350 |
| BseRI | GAGGAG | 2 cut(s) | 935, 1533 |
| BseSI | GKGCMC | 3 cut(s) | 964, 2557, 2865 |
| BseXI | GCAGC | 8 cut(s) | 224, 246, 392, 484, 1098, 3302, 3393, 3556 |
| BseYI | CCCAGC | 1 cut(s) | 2520 |
| BsgI | GTGCAG | 1 cut(s) | 3309 |
| Bsh1236I | CGCG | 1 cut(s) | 1633 |
| BshFI | GGCC | 8 cut(s) | 312, 1157, 1355, 1707, 2050, 2335, 3211, 3437 |
| BsiHKAI | GWGCWC | 5 cut(s) | 31, 721, 964, 2557, 2865 |
| BsiSI | CCGG | 4 cut(s) | 953, 1490, 2195, 2969 |
| BslFI | GGGAC | 3 cut(s) | 1394, 1767, 2579 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 2596 |
| BsmAI | GTCTC | 3 cut(s) | 1524, 1603, 2846 |
| BsmFI | GGGAC | 3 cut(s) | 1394, 1767, 2579 |
| BsmI | GAATGC | 3 cut(s) | 1275, 2719, 3061 |
| BsnI | GGCC | 8 cut(s) | 312, 1157, 1355, 1707, 2050, 2335, 3211, 3437 |
| Bso31I | GGTCTC | 1 cut(s) | 1524 |
| Bsp119I | TTCGAA | 4 cut(s) | 854, 1053, 2261, 2485 |
| Bsp1286I | GDGCHC | 5 cut(s) | 31, 721, 964, 2557, 2865 |
| Bsp13I | TCCGGA | 1 cut(s) | 952 |
| Bsp1720I | GCTNAGC | 2 cut(s) | 832, 3402 |
| Bsp19I | CCATGG | 3 cut(s) | 2253, 2699, 3524 |
| BspACI | CCGC | 5 cut(s) | 836, 1393, 1631, 1813, 3368 |
| BspANI | GGCC | 8 cut(s) | 312, 1157, 1355, 1707, 2050, 2335, 3211, 3437 |
| BspCNI | CTCAG | 7 cut(s) | 845, 1282, 1792, 2065, 2756, 3004, 3394 |
| BspEI | TCCGGA | 1 cut(s) | 952 |
| BspFNI | CGCG | 1 cut(s) | 1633 |
| BspHI | TCATGA | 2 cut(s) | 31, 2356 |
| BspLI | GGNNCC | 1 cut(s) | 951 |
| BspMAI | CTGCAG | 1 cut(s) | 2860 |
| BspMI | ACCTGC | 1 cut(s) | 217 |
| BspPI | GGATC | 4 cut(s) | 573, 633, 944, 957 |
| BspQI | GCTCTTC | 1 cut(s) | 1104 |
| BspT104I | TTCGAA | 4 cut(s) | 854, 1053, 2261, 2485 |
| BspTI | CTTAAG | 1 cut(s) | 2183 |
| BspTNI | GGTCTC | 1 cut(s) | 1524 |
| BsrDI | GCAATG | 2 cut(s) | 93, 1958 |
| BsrFI | RCCGGY | 1 cut(s) | 1489 |
| BsrI | ACTGG | 2 cut(s) | 688, 1350 |
| BssAI | RCCGGY | 1 cut(s) | 1489 |
| BssECI | CCNNGG | 8 cut(s) | 209, 642, 879, 1525, 2253, 2699, 2735, 3524 |
| BssSI | CACGAG | 2 cut(s) | 510, 3294 |
| BssT1I | CCWWGG | 6 cut(s) | 209, 642, 2253, 2699, 2735, 3524 |
| Bst2BI | CACGAG | 2 cut(s) | 510, 3294 |
| Bst2UI | CCWGG | 6 cut(s) | 881, 966, 1526, 2047, 2337, 3306 |
| Bst4CI | ACNGT | 9 cut(s) | 998, 1758, 2134, 2379, 2470, 2653, 3001, 3166, 3554 |
| Bst6I | CTCTTC | 3 cut(s) | 1104, 2251, 2873 |
| BstAFI | CTTAAG | 1 cut(s) | 2183 |
| BstBAI | YACGTR | 1 cut(s) | 650 |
| BstBI | TTCGAA | 4 cut(s) | 854, 1053, 2261, 2485 |
| BstC8I | GCNNGC | 7 cut(s) | 1487, 1923, 2018, 3028, 3048, 3209, 3411 |
| BstDSI | CCRYGG | 3 cut(s) | 2253, 2699, 3524 |
| BstEII | GGTNACC | 1 cut(s) | 2807 |
| BstFNI | CGCG | 1 cut(s) | 1633 |
| BstH2I | RGCGCY | 1 cut(s) | 832 |
| BstHHI | GCGC | 2 cut(s) | 831, 2679 |
| BstMAI | GTCTC | 3 cut(s) | 1524, 1603, 2846 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 506, 596, 3056, 3398, 3566 |
| BstNI | CCWGG | 6 cut(s) | 881, 966, 1526, 2047, 2337, 3306 |
| BstNSI | RCATGY | 5 cut(s) | 791, 2020, 3050, 3251, 3581 |
| BstPI | GGTNACC | 1 cut(s) | 2807 |
| BstSCI | CCNGG | 7 cut(s) | 879, 964, 1524, 2045, 2193, 2335, 3304 |
| BstSFI | CTRYAG | 3 cut(s) | 2466, 2475, 2856 |
| BstSLI | GKGCMC | 3 cut(s) | 964, 2557, 2865 |
| BstUI | CGCG | 1 cut(s) | 1633 |
| BstV1I | GCAGC | 8 cut(s) | 224, 246, 392, 484, 1098, 3302, 3393, 3556 |
| BstV2I | GAAGAC | 1 cut(s) | 378 |
| BstX2I | RGATCY | 1 cut(s) | 949 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 3433 |
| BstYI | RGATCY | 1 cut(s) | 949 |
| BsuI | GTATCC | 1 cut(s) | 832 |
| BsuRI | GGCC | 8 cut(s) | 312, 1157, 1355, 1707, 2050, 2335, 3211, 3437 |
| BtgI | CCRYGG | 3 cut(s) | 2253, 2699, 3524 |
| BtgZI | GCGATG | 2 cut(s) | 87, 3489 |
| BtrI | CACGTC | 1 cut(s) | 716 |
| BtsI | GCAGTG | 1 cut(s) | 2865 |
| BtsIMutI | CAGTG | 4 cut(s) | 2010, 2064, 2384, 2865 |
| BveI | ACCTGC | 1 cut(s) | 217 |
| Cac8I | GCNNGC | 7 cut(s) | 1487, 1923, 2018, 3028, 3048, 3209, 3411 |
| CciI | TCATGA | 2 cut(s) | 31, 2356 |
| CfoI | GCGC | 2 cut(s) | 831, 2679 |
| Cfr10I | RCCGGY | 1 cut(s) | 1489 |
| Cfr13I | GGNCC | 4 cut(s) | 311, 968, 1019, 2926 |
| CsiI | ACCWGGT | 1 cut(s) | 964 |
| Csp6I | GTAC | 4 cut(s) | 2934, 2972, 3144, 3150 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 382, 417 |
| CviQI | GTAC | 4 cut(s) | 2934, 2972, 3144, 3150 |
| DraI | TTTAAA | 1 cut(s) | 1171 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 2573 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 2573 |
| EaeI | YGGCCR | 2 cut(s) | 1155, 1353 |
| Eam1104I | CTCTTC | 3 cut(s) | 1104, 2251, 2873 |
| EarI | CTCTTC | 3 cut(s) | 1104, 2251, 2873 |
| Ecl136II | GAGCTC | 1 cut(s) | 29 |
| Eco130I | CCWWGG | 6 cut(s) | 209, 642, 2253, 2699, 2735, 3524 |
| Eco147I | AGGCCT | 2 cut(s) | 1707, 3211 |
| Eco24I | GRGCYC | 1 cut(s) | 31 |
| Eco31I | GGTCTC | 1 cut(s) | 1524 |
| Eco32I | GATATC | 2 cut(s) | 121, 1967 |
| Eco47I | GGWCC | 3 cut(s) | 968, 1019, 2926 |
| Eco53kI | GAGCTC | 1 cut(s) | 29 |
| Eco57I | CTGAAG | 2 cut(s) | 1486, 1598 |
| Eco72I | CACGTG | 1 cut(s) | 650 |
| Eco91I | GGTNACC | 1 cut(s) | 2807 |
| EcoICRI | GAGCTC | 1 cut(s) | 29 |
| EcoO65I | GGTNACC | 1 cut(s) | 2807 |
| EcoRI | GAATTC | 1 cut(s) | 3454 |
| EcoRII | CCWGG | 6 cut(s) | 879, 964, 1524, 2045, 2335, 3304 |
| EcoRV | GATATC | 2 cut(s) | 121, 1967 |
| EcoT14I | CCWWGG | 6 cut(s) | 209, 642, 2253, 2699, 2735, 3524 |
| EcoT22I | ATGCAT | 1 cut(s) | 3579 |
| EcoT38I | GRGCYC | 1 cut(s) | 31 |
| ErhI | CCWWGG | 6 cut(s) | 209, 642, 2253, 2699, 2735, 3524 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 1844, 1876, 2160, 2192 |
| FaqI | GGGAC | 3 cut(s) | 1394, 1767, 2579 |
| FauI | CCCGC | 2 cut(s) | 1386, 3361 |
| FauNDI | CATATG | 1 cut(s) | 2665 |
| FbaI | TGATCA | 2 cut(s) | 265, 1128 |
| FblI | GTMKAC | 1 cut(s) | 638 |
| Fnu4HI | GCNGC | 8 cut(s) | 235, 238, 406, 498, 1087, 3291, 3407, 3570 |
| FriOI | GRGCYC | 1 cut(s) | 31 |
| Fsp4HI | GCNGC | 8 cut(s) | 235, 238, 406, 498, 1087, 3291, 3407, 3570 |
| FspBI | CTAG | 8 cut(s) | 932, 1559, 1650, 2118, 2724, 2736, 3051, 3628 |
| GlaI | GCGC | 2 cut(s) | 830, 2678 |
| GluI | GCNGC | 8 cut(s) | 235, 238, 406, 498, 1087, 3291, 3407, 3570 |
| GsaI | CCCAGC | 1 cut(s) | 2524 |
| GsuI | CTGGAG | 1 cut(s) | 902 |
| HaeII | RGCGCY | 1 cut(s) | 832 |
| HaeIII | GGCC | 8 cut(s) | 312, 1157, 1355, 1707, 2050, 2335, 3211, 3437 |
| HapII | CCGG | 4 cut(s) | 953, 1490, 2195, 2969 |
| HhaI | GCGC | 2 cut(s) | 831, 2679 |
| Hin6I | GCGC | 2 cut(s) | 829, 2677 |
| HinP1I | GCGC | 2 cut(s) | 829, 2677 |
| HincII | GTYRAC | 3 cut(s) | 1324, 2241, 2997 |
| HindII | GTYRAC | 3 cut(s) | 1324, 2241, 2997 |
| HindIII | AAGCTT | 4 cut(s) | 1359, 1463, 1610, 1853 |
| HpaI | GTTAAC | 2 cut(s) | 2241, 2997 |
| HpaII | CCGG | 4 cut(s) | 953, 1490, 2195, 2969 |
| HphI | GGTGA | 4 cut(s) | 17, 382, 683, 2801 |
| Hpy99I | CGWCG | 3 cut(s) | 717, 1812, 2805 |
| HpyAV | CCTTC | 6 cut(s) | 324, 2070, 2162, 2965, 3222, 3598 |
| HpyCH4III | ACNGT | 9 cut(s) | 998, 1758, 2134, 2379, 2470, 2653, 3001, 3166, 3554 |
| HpyCH4IV | ACGT | 6 cut(s) | 476, 649, 715, 2800, 2946, 2974 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 506, 596, 3056, 3398, 3566 |
| HpySE526I | ACGT | 6 cut(s) | 476, 649, 715, 2800, 2946, 2974 |
| HspAI | GCGC | 2 cut(s) | 829, 2677 |
| Kpn2I | TCCGGA | 1 cut(s) | 952 |
| Ksp22I | TGATCA | 2 cut(s) | 265, 1128 |
| KspAI | GTTAAC | 2 cut(s) | 2241, 2997 |
| LguI | GCTCTTC | 1 cut(s) | 1104 |
| LmnI | GCTCC | 5 cut(s) | 26, 1651, 2834, 3023, 3226 |
| Lsp1109I | GCAGC | 8 cut(s) | 224, 246, 392, 484, 1098, 3302, 3393, 3556 |
| LweI | GCATC | 6 cut(s) | 358, 753, 1230, 2425, 3105, 3196 |
| MabI | ACCWGGT | 1 cut(s) | 964 |
| MaeI | CTAG | 8 cut(s) | 932, 1559, 1650, 2118, 2724, 2736, 3051, 3628 |
| MaeII | ACGT | 6 cut(s) | 476, 649, 715, 2800, 2946, 2974 |
| MaeIII | GTNAC | 6 cut(s) | 342, 671, 874, 1420, 2775, 2807 |
| MfeI | CAATTG | 4 cut(s) | 1146, 1209, 1845, 2889 |
| MflI | RGATCY | 1 cut(s) | 949 |
| MhlI | GDGCHC | 5 cut(s) | 31, 721, 964, 2557, 2865 |
| MlsI | TGGCCA | 2 cut(s) | 1157, 1355 |
| MluNI | TGGCCA | 2 cut(s) | 1157, 1355 |
| MlyI | GAGTC | 3 cut(s) | 1948, 2698, 2914 |
| MmeI | TCCRAC | 4 cut(s) | 986, 2046, 2486, 3431 |
| Mox20I | TGGCCA | 2 cut(s) | 1157, 1355 |
| Mph1103I | ATGCAT | 1 cut(s) | 3579 |
| MroI | TCCGGA | 1 cut(s) | 952 |
| MroXI | GAANNNNTTC | 1 cut(s) | 2494 |
| MscI | TGGCCA | 2 cut(s) | 1157, 1355 |
| MslI | CAYNNNNRTG | 2 cut(s) | 2824, 2844 |
| Msp20I | TGGCCA | 2 cut(s) | 1157, 1355 |
| MspA1I | CMGCKG | 3 cut(s) | 237, 836, 1393 |
| MspCI | CTTAAG | 1 cut(s) | 2183 |
| MspI | CCGG | 4 cut(s) | 953, 1490, 2195, 2969 |
| MspR9I | CCNGG | 7 cut(s) | 881, 966, 1526, 2047, 2195, 2337, 3306 |
| MunI | CAATTG | 4 cut(s) | 1146, 1209, 1845, 2889 |
| Mva1269I | GAATGC | 3 cut(s) | 1275, 2719, 3061 |
| MvaI | CCWGG | 6 cut(s) | 881, 966, 1526, 2047, 2337, 3306 |
| MvnI | CGCG | 1 cut(s) | 1633 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 506, 596, 3056, 3398, 3566 |
| NciI | CCSGG | 1 cut(s) | 2195 |
| NcoI | CCATGG | 3 cut(s) | 2253, 2699, 3524 |
| NdeI | CATATG | 1 cut(s) | 2665 |
| NlaIV | GGNNCC | 1 cut(s) | 951 |
| NmeAIII | GCCGAG | 1 cut(s) | 3114 |
| NmuCI | GTSAC | 2 cut(s) | 671, 2807 |
| NsiI | ATGCAT | 1 cut(s) | 3579 |
| NspI | RCATGY | 5 cut(s) | 791, 2020, 3050, 3251, 3581 |
| NspV | TTCGAA | 4 cut(s) | 854, 1053, 2261, 2485 |
| PaeI | GCATGC | 2 cut(s) | 2020, 3050 |
| PagI | TCATGA | 2 cut(s) | 31, 2356 |
| PceI | AGGCCT | 2 cut(s) | 1707, 3211 |
| PciSI | GCTCTTC | 1 cut(s) | 1104 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 2800 |
| PctI | GAATGC | 3 cut(s) | 1275, 2719, 3061 |
| PdmI | GAANNNNTTC | 1 cut(s) | 2494 |
| PfeI | GAWTC | 7 cut(s) | 218, 466, 490, 1055, 1446, 3074, 3476 |
| PflFI | GACNNNGTC | 1 cut(s) | 2799 |
| PkrI | GCNGC | 8 cut(s) | 236, 239, 407, 499, 1088, 3292, 3408, 3571 |
| PleI | GAGTC | 3 cut(s) | 1947, 2698, 2913 |
| PmaCI | CACGTG | 1 cut(s) | 650 |
| PmlI | CACGTG | 1 cut(s) | 650 |
| PpsI | GAGTC | 3 cut(s) | 1947, 2698, 2913 |
| Ppu21I | YACGTR | 1 cut(s) | 650 |
| PshBI | ATTAAT | 2 cut(s) | 2546, 3132 |
| PsiI | TTATAA | 2 cut(s) | 573, 2316 |
| Psp124BI | GAGCTC | 1 cut(s) | 31 |
| Psp6I | CCWGG | 6 cut(s) | 879, 964, 1524, 2045, 2335, 3304 |
| PspCI | CACGTG | 1 cut(s) | 650 |
| PspEI | GGTNACC | 1 cut(s) | 2807 |
| PspFI | CCCAGC | 1 cut(s) | 2520 |
| PspGI | CCWGG | 6 cut(s) | 879, 964, 1524, 2045, 2335, 3304 |
| PspN4I | GGNNCC | 1 cut(s) | 951 |
| PspPI | GGNCC | 4 cut(s) | 311, 968, 1019, 2926 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 1023, 1055 |
| PstI | CTGCAG | 1 cut(s) | 2860 |
| PsuI | RGATCY | 1 cut(s) | 949 |
| PsyI | GACNNNGTC | 1 cut(s) | 2799 |
| PvuII | CAGCTG | 1 cut(s) | 237 |
| RsaI | GTAC | 4 cut(s) | 2935, 2973, 3145, 3151 |
| RsaNI | GTAC | 4 cut(s) | 2934, 2972, 3144, 3150 |
| RseI | CAYNNNNRTG | 2 cut(s) | 2824, 2844 |
| SacI | GAGCTC | 1 cut(s) | 31 |
| SapI | GCTCTTC | 1 cut(s) | 1104 |
| SatI | GCNGC | 8 cut(s) | 235, 238, 406, 498, 1087, 3291, 3407, 3570 |
| Sau96I | GGNCC | 4 cut(s) | 311, 968, 1019, 2926 |
| ScaI | AGTACT | 1 cut(s) | 2935 |
| SchI | GAGTC | 3 cut(s) | 1948, 2698, 2914 |
| ScrFI | CCNGG | 7 cut(s) | 881, 966, 1526, 2047, 2195, 2337, 3306 |
| SduI | GDGCHC | 5 cut(s) | 31, 721, 964, 2557, 2865 |
| SexAI | ACCWGGT | 1 cut(s) | 964 |
| SfaNI | GCATC | 6 cut(s) | 358, 753, 1230, 2425, 3105, 3196 |
| SfcI | CTRYAG | 3 cut(s) | 2466, 2475, 2856 |
| SfuI | TTCGAA | 4 cut(s) | 854, 1053, 2261, 2485 |
| SinI | GGWCC | 3 cut(s) | 968, 1019, 2926 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 2824, 2844 |
| SmlI | CTYRAG | 3 cut(s) | 455, 521, 2183 |
| SmoI | CTYRAG | 3 cut(s) | 455, 521, 2183 |
| SpeI | ACTAGT | 1 cut(s) | 2117 |
| SphI | GCATGC | 2 cut(s) | 2020, 3050 |
| SseBI | AGGCCT | 2 cut(s) | 1707, 3211 |
| SsiI | CCGC | 5 cut(s) | 836, 1393, 1631, 1813, 3368 |
| SspI | AATATT | 3 cut(s) | 775, 1333, 2458 |
| SspMI | CTAG | 8 cut(s) | 932, 1559, 1650, 2118, 2724, 2736, 3051, 3628 |
| SstI | GAGCTC | 1 cut(s) | 31 |
| StuI | AGGCCT | 2 cut(s) | 1707, 3211 |
| StyD4I | CCNGG | 7 cut(s) | 879, 964, 1524, 2045, 2193, 2335, 3304 |
| StyI | CCWWGG | 6 cut(s) | 209, 642, 2253, 2699, 2735, 3524 |
| TaaI | ACNGT | 9 cut(s) | 998, 1758, 2134, 2379, 2470, 2653, 3001, 3166, 3554 |
| TaiI | ACGT | 6 cut(s) | 479, 652, 718, 2803, 2949, 2977 |
| TaqI | TCGA | 9 cut(s) | 712, 795, 854, 1053, 2261, 2485, 2794, 3458, 3556 |
| TaqII | GACCGA | 1 cut(s) | 711 |
| TatI | WGTACW | 1 cut(s) | 2933 |
| TfiI | GAWTC | 7 cut(s) | 218, 466, 490, 1055, 1446, 3074, 3476 |
| TscAI | CASTG | 4 cut(s) | 2010, 2064, 2384, 2865 |
| TseFI | GTSAC | 2 cut(s) | 671, 2807 |
| TseI | GCWGC | 8 cut(s) | 234, 237, 405, 497, 1086, 3290, 3406, 3569 |
| Tsp45I | GTSAC | 2 cut(s) | 671, 2807 |
| TspGWI | ACGGA | 2 cut(s) | 575, 855 |
| TspRI | CASTG | 4 cut(s) | 2010, 2064, 2384, 2865 |
| Tth111I | GACNNNGTC | 1 cut(s) | 2799 |
| Vha464I | CTTAAG | 1 cut(s) | 2183 |
| VneI | GTGCAC | 3 cut(s) | 960, 2553, 2861 |
| VpaK11BI | GGWCC | 3 cut(s) | 968, 1019, 2926 |
| VspI | ATTAAT | 2 cut(s) | 2546, 3132 |
| XceI | RCATGY | 5 cut(s) | 791, 2020, 3050, 3251, 3581 |
| XmaJI | CCTAGG | 1 cut(s) | 2735 |
| XmiI | GTMKAC | 1 cut(s) | 638 |
| XmnI | GAANNNNTTC | 1 cut(s) | 2494 |
| XspI | CTAG | 8 cut(s) | 932, 1559, 1650, 2118, 2724, 2736, 3051, 3628 |
| ZrmI | AGTACT | 1 cut(s) | 2935 |
| Zsp2I | ATGCAT | 1 cut(s) | 3579 |