Rorug05G0512200
HSP70 Family

Belongs to the heat shock protein 70 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0021447
Physical Location & Seq
Forward (+)
69645749 .. 69650183
4435 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0512200.1

Sequence Viewer

Length: 1503 bp
ATGGCCTCTGCTCAGGGCCTTGCTTCTTTGACCCAGACGTGTTTTGCAGTTAAACGGTACCGTTTTAGCGGTTCAAAGGGGTTTTCAGCAAATTCTTGTGCTAGGGTTTGTTCTGTAATCTGCAAACAGCAGAATACCAACTTTGTGGTTGCGAAATCGAGTAAAGTTCGTGACTTTAGGATTTTTAAATCGGTTCAGTTGGACCAGTTTGTGACTAGTGACGATGAGGATGAGATGGGTGAGAGTTTCTTTGAGGCAATTGAGGAGTTAGAGAGGATGAGAAGGGAGCCGTCTGATGTTCTTGAGGAAATGAATGACCGGTTATCGGCGAGAGAGCTACAGCTTGTGTTGGTGTATTTTTCGCAGGAGGGTAGGGACTCGTGGTGTGCACTTGAGGTGTTTGAGTGGCTCCGGAAGGAGAACCGGGTCGATAAGGAGACCATGGAGCTTATGGTTTCGATAATGTGTGGTTGGTTGAAGAGGTTGATTGAGGAGGGGAATGATGTCGCTGATGTGATTGACCTTCTGGTGGATGTGGATTGTGTTGGTTTGAAGCCGAGTTTTAGCATGATGGAGAAGGTGATTTCGTTGTATTGGGAGATGGGGGAGAAGGAGAATGCGGTTTTGTTTGTGAAAGAAGTGTTGAAACGCGGGATTGTGTATTCTGAGGAGGATGATCGAGATGGACATAAAGGAGGACCAACTGGGTATCTTGCTTGGAAGATGATGGTTGACGGTAACTATAGGGATTCAGTGAAGTTTGTAATTCAACTAAGAGAATCTGGGTTGAAGCCAGAGGTTTACAGCTACCTCATTGCAATGACAGCTGTGGTTAAAGAACTAAATGAGCTTGGGAAAGCTTTACGCAAGTTGAAAGCTCTTACAAGGGCTGGGCTAGTGGCTGAGTTTGATTCAGAAGATGTTGGGCTAATTGAGAAGTATCAGTCGGATCTTCTAGCTGATGGATTACAATTGTCTAATTGGGTGATTCAAGAGGGAAGTTCTTCACTCTGTGGGGTGGTTCATGAGAGGCTTCTGGCTATGTATATCTGTTCTGGACGTGGACTTGAGGCCGAAAGACAGTTGTGGGAAATGAAGCTTGTGGGTAAAGAGCCTGATGGGGACCTTTATGACATTGTTTTAGCCATCTGTGCTTCCCGCAAGGAAACCAATGCCATTGCAAGGTTGCTTACTAGGACAGAGGTTAGCAGCTCTTTATGTAAGAAGAAAAGCCTCTCATGGCTGTTAAGAGGTTACATTAAAGGAGGACATTTTGATGATGCTGCAGAAACAGTAATTAAAATGCTTGATTTGGGTTTGTTCCCGGAGTATTTGGATAGAGCAGCTGTGTTGCATGGACTACGGAAAAGGATTCAACAGTCTGGTACTGTAGATACTTACCTCAAACTTTGCAAGCGCCTTTCAGATGCCAATTTGATTGAATCCTGTCTCGTATATCTGTATATAAAGAAACATAAGCTTTGGATCATAAAGACGGTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 ko04010 ko04141 ko04144 ko04213 ko04612 ko04915 ko05134 ko05145 ko05162 ko05164 ko05169 map03040 map04010 map04141 map04144 map04213 map04612 map04915 map05134 map05145 map05162 map05164 map05169
Pfam Domains
Protein Families
HSP70

Protein Analysis

500

Amino Acids

56.79

Weight (kDa)

5.63

Isoelectric Point (pI)

30.83

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0021447)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr6g0245101 RchiOBHm_Chr6g0245111 RchiOBHm_Chr6g0263441
rosa_rugosa Rorug05G0512200
rosa_samantha Rh6BG022900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 57
AccB1I GGYRCC 1 cut(s) 57
AccII CGCG 1 cut(s) 651
AccIII TCCGGA 1 cut(s) 411
AciI CCGC 4 cut(s) 69, 620, 651, 1159
AclWI GGATC 2 cut(s) 957, 1493
AcsI RAATTY 1 cut(s) 91
AdeI CACNNNGTG 1 cut(s) 1013
AfaI GTAC 2 cut(s) 59, 1387
AfiI CCNNNNNNNGG 3 cut(s) 325, 529, 1182
AflIII ACRYGT 1 cut(s) 38
AgeI ACCGGT 1 cut(s) 318
AhlI ACTAGT 1 cut(s) 215
AjiI CACGTC 2 cut(s) 39, 1061
Alw21I GWGCWC 1 cut(s) 391
Alw26I GTCTC 2 cut(s) 431, 1454
Alw44I GTGCAC 1 cut(s) 387
AlwI GGATC 2 cut(s) 957, 1493
Aor13HI TCCGGA 1 cut(s) 411
AoxI GGCC 3 cut(s) 3, 16, 1071
ApaLI GTGCAC 1 cut(s) 387
ApeKI GCWGC 3 cut(s) 1209, 1283, 1343
ApoI RAATTY 1 cut(s) 91
AsiGI ACCGGT 1 cut(s) 318
Asp700I GAANNNNTTC 1 cut(s) 1003
Asp718I GGTACC 1 cut(s) 57
AspLEI GCGC 1 cut(s) 1419
AspS9I GGNCC 4 cut(s) 16, 202, 698, 1123
AsuC2I CCSGG 2 cut(s) 425, 1325
AsuHPI GGTGA 3 cut(s) 251, 592, 997
AvaII GGWCC 3 cut(s) 202, 698, 1123
BaeGI GKGCMC 1 cut(s) 391
BanI GGYRCC 1 cut(s) 57
BauI CACGAG 1 cut(s) 379
Bbv12I GWGCWC 1 cut(s) 391
BbvI GCAGC 3 cut(s) 1221, 1270, 1355
BccI CCATC 8 cut(s) 229, 565, 595, 677, 721, 956, 1112, 1154
BceAI ACGGC 1 cut(s) 274
BcnI CCSGG 2 cut(s) 425, 1325
BcoDI GTCTC 2 cut(s) 431, 1454
BcuI ACTAGT 1 cut(s) 215
BfaI CTAG 6 cut(s) 102, 216, 896, 956, 1194, 1501
BfmI CTRYAG 4 cut(s) 338, 742, 1284, 1389
BfoI RGCGCY 1 cut(s) 1420
BisI GCNGC 3 cut(s) 1210, 1284, 1344
BlsI GCNGC 3 cut(s) 1211, 1285, 1345
Bme1390I CCNGG 2 cut(s) 425, 1325
Bme18I GGWCC 3 cut(s) 202, 698, 1123
BmgBI CACGTC 2 cut(s) 39, 1061
BmgT120I GGNCC 4 cut(s) 16, 202, 698, 1123
BmiI GGNNCC 4 cut(s) 59, 288, 410, 1124
BmrFI CCNGG 2 cut(s) 425, 1325
BmrI ACTGGG 1 cut(s) 714
BmsI GCATC 2 cut(s) 1270, 1417
BmuI ACTGGG 1 cut(s) 714
Bpu10I CCTNAGC 1 cut(s) 12
BpuEI CTTGAG 3 cut(s) 323, 413, 1088
BpuMI CCSGG 2 cut(s) 425, 1325
BsaI GGTCTC 1 cut(s) 431
BsaJI CCNNGG 1 cut(s) 441
BsaWI WCCGGW 2 cut(s) 318, 411
Bsc4I CCNNNNNNNGG 3 cut(s) 325, 529, 1182
Bse118I RCCGGY 1 cut(s) 318
Bse1I ACTGG 2 cut(s) 205, 709
Bse3DI GCAATG 3 cut(s) 813, 825, 1176
BseAI TCCGGA 1 cut(s) 411
BseDI CCNNGG 1 cut(s) 441
BseGI GGATG 4 cut(s) 235, 282, 538, 679
BseLI CCNNNNNNNGG 3 cut(s) 325, 529, 1182
BseMI GCAATG 3 cut(s) 813, 825, 1176
BseMII CTCAG 3 cut(s) 26, 657, 894
BseNI ACTGG 2 cut(s) 205, 709
BseRI GAGGAG 3 cut(s) 278, 506, 683
BseSI GKGCMC 1 cut(s) 391
BseXI GCAGC 3 cut(s) 1221, 1270, 1355
BseYI CCCAGC 1 cut(s) 890
Bsh1236I CGCG 1 cut(s) 651
BshFI GGCC 3 cut(s) 5, 18, 1073
BshNI GGYRCC 1 cut(s) 57
BshTI ACCGGT 1 cut(s) 318
BsiHKAI GWGCWC 1 cut(s) 391
BsiSI CCGG 4 cut(s) 319, 412, 424, 1325
BslFI GGGAC 2 cut(s) 389, 1136
BslI CCNNNNNNNGG 3 cut(s) 325, 529, 1182
BsmAI GTCTC 2 cut(s) 431, 1454
BsmFI GGGAC 2 cut(s) 389, 1136
BsmI GAATGC 1 cut(s) 622
BsnI GGCC 3 cut(s) 5, 18, 1073
Bso31I GGTCTC 1 cut(s) 431
Bsp1286I GDGCHC 1 cut(s) 391
Bsp13I TCCGGA 1 cut(s) 411
Bsp143I GATC 3 cut(s) 676, 949, 1485
Bsp19I CCATGG 1 cut(s) 441
BspACI CCGC 4 cut(s) 69, 620, 651, 1159
BspANI GGCC 3 cut(s) 5, 18, 1073
BspCNI CTCAG 3 cut(s) 25, 658, 895
BspEI TCCGGA 1 cut(s) 411
BspFNI CGCG 1 cut(s) 651
BspHI TCATGA 1 cut(s) 1024
BspLI GGNNCC 4 cut(s) 59, 288, 410, 1124
BspMAI CTGCAG 1 cut(s) 1288
BspPI GGATC 2 cut(s) 957, 1493
BspT107I GGYRCC 1 cut(s) 57
BspTNI GGTCTC 1 cut(s) 431
BsrDI GCAATG 3 cut(s) 813, 825, 1176
BsrFI RCCGGY 1 cut(s) 318
BsrI ACTGG 2 cut(s) 205, 709
BssAI RCCGGY 1 cut(s) 318
BssECI CCNNGG 1 cut(s) 441
BssMI GATC 3 cut(s) 676, 949, 1485
BssSI CACGAG 1 cut(s) 379
BssT1I CCWWGG 1 cut(s) 441
Bst2BI CACGAG 1 cut(s) 379
Bst4CI ACNGT 8 cut(s) 57, 62, 737, 1083, 1294, 1380, 1390, 1498
Bst6I CTCTTC 1 cut(s) 473
BstC8I GCNNGC 1 cut(s) 1415
BstDEI CTNAG 4 cut(s) 12, 666, 773, 903
BstDSI CCRYGG 1 cut(s) 441
BstF5I GGATG 4 cut(s) 235, 282, 538, 679
BstFNI CGCG 1 cut(s) 651
BstH2I RGCGCY 1 cut(s) 1420
BstHHI GCGC 1 cut(s) 1419
BstKTI GATC 3 cut(s) 679, 952, 1488
BstMAI GTCTC 2 cut(s) 431, 1454
BstMBI GATC 3 cut(s) 676, 949, 1485
BstMWI GCNNNNNNNGC 2 cut(s) 824, 1151
BstSCI CCNGG 2 cut(s) 423, 1323
BstSFI CTRYAG 4 cut(s) 338, 742, 1284, 1389
BstSLI GKGCMC 1 cut(s) 391
BstUI CGCG 1 cut(s) 651
BstV1I GCAGC 3 cut(s) 1221, 1270, 1355
BstX2I RGATCY 1 cut(s) 949
BstXI CCANNNNNNTGG 1 cut(s) 145
BstYI RGATCY 1 cut(s) 949
BsuRI GGCC 3 cut(s) 5, 18, 1073
BtgI CCRYGG 1 cut(s) 441
BtrI CACGTC 2 cut(s) 39, 1061
BtsCI GGATG 4 cut(s) 235, 282, 538, 679
BtsIMutI CAGTG 1 cut(s) 759
Cac8I GCNNGC 1 cut(s) 1415
CciI TCATGA 1 cut(s) 1024
CfoI GCGC 1 cut(s) 1419
Cfr10I RCCGGY 1 cut(s) 318
Cfr13I GGNCC 4 cut(s) 16, 202, 698, 1123
Csp6I GTAC 2 cut(s) 58, 1386
CspAI ACCGGT 1 cut(s) 318
CviAII CATG 5 cut(s) 442, 568, 1025, 1239, 1355
CviQI GTAC 2 cut(s) 58, 1386
DdeI CTNAG 4 cut(s) 12, 666, 773, 903
DpnI GATC 3 cut(s) 678, 951, 1487
DpnII GATC 3 cut(s) 676, 949, 1485
DraI TTTAAA 1 cut(s) 187
DraIII CACNNNGTG 1 cut(s) 1013
Eam1104I CTCTTC 1 cut(s) 473
EarI CTCTTC 1 cut(s) 473
Eco130I CCWWGG 1 cut(s) 441
Eco31I GGTCTC 1 cut(s) 431
Eco47I GGWCC 3 cut(s) 202, 698, 1123
EcoO109I RGGNCCY 2 cut(s) 16, 1123
EcoT14I CCWWGG 1 cut(s) 441
ErhI CCWWGG 1 cut(s) 441
FaeI CATG 5 cut(s) 445, 571, 1028, 1242, 1358
FaqI GGGAC 2 cut(s) 389, 1136
FatI CATG 5 cut(s) 441, 567, 1024, 1238, 1354
FauI CCCGC 2 cut(s) 644, 1166
Fnu4HI GCNGC 3 cut(s) 1210, 1284, 1344
FokI GGATG 4 cut(s) 242, 289, 545, 686
Fsp4HI GCNGC 3 cut(s) 1210, 1284, 1344
FspBI CTAG 6 cut(s) 102, 216, 896, 956, 1194, 1501
GlaI GCGC 1 cut(s) 1418
GluI GCNGC 3 cut(s) 1210, 1284, 1344
GsaI CCCAGC 1 cut(s) 894
HaeII RGCGCY 1 cut(s) 1420
HaeIII GGCC 3 cut(s) 5, 18, 1073
HapII CCGG 4 cut(s) 319, 412, 424, 1325
HhaI GCGC 1 cut(s) 1419
Hin1II CATG 5 cut(s) 445, 571, 1028, 1242, 1358
Hin6I GCGC 1 cut(s) 1417
HinP1I GCGC 1 cut(s) 1417
HincII GTYRAC 1 cut(s) 733
HindII GTYRAC 1 cut(s) 733
HindIII AAGCTT 3 cut(s) 858, 1097, 1478
HinfI GANTC 7 cut(s) 377, 749, 779, 911, 988, 1372, 1442
HpaII CCGG 4 cut(s) 319, 412, 424, 1325
HphI GGTGA 3 cut(s) 251, 592, 997
Hpy166II GTNNAC 4 cut(s) 389, 733, 802, 1064
Hpy188I TCNGA 5 cut(s) 295, 667, 916, 949, 1426
Hpy188III TCNNGA 7 cut(s) 170, 302, 412, 680, 992, 1025, 1056
Hpy8I GTNNAC 4 cut(s) 389, 733, 802, 1064
HpyAV CCTTC 5 cut(s) 276, 409, 533, 571, 604
HpyCH4III ACNGT 8 cut(s) 57, 62, 737, 1083, 1294, 1380, 1390, 1498
HpyCH4IV ACGT 2 cut(s) 38, 1060
HpyCH4V TGCA 8 cut(s) 47, 123, 389, 818, 1181, 1286, 1354, 1413
HpyF10VI GCNNNNNNNGC 2 cut(s) 824, 1151
HpyF3I CTNAG 4 cut(s) 12, 666, 773, 903
HpySE526I ACGT 2 cut(s) 38, 1060
Hsp92II CATG 5 cut(s) 445, 571, 1028, 1242, 1358
HspAI GCGC 1 cut(s) 1417
Kpn2I TCCGGA 1 cut(s) 411
KpnI GGTACC 1 cut(s) 61
Kzo9I GATC 3 cut(s) 676, 949, 1485
LmnI GCTCC 3 cut(s) 286, 414, 445
Lsp1109I GCAGC 3 cut(s) 1221, 1270, 1355
LweI GCATC 2 cut(s) 1270, 1417
MaeI CTAG 6 cut(s) 102, 216, 896, 956, 1194, 1501
MaeII ACGT 2 cut(s) 38, 1060
MaeIII GTNAC 5 cut(s) 170, 211, 218, 737, 1253
MalI GATC 3 cut(s) 678, 951, 1487
MboI GATC 3 cut(s) 676, 949, 1485
MboII GAAGA 6 cut(s) 490, 733, 929, 944, 996, 1237
MfeI CAATTG 2 cut(s) 258, 971
MflI RGATCY 1 cut(s) 949
MhlI GDGCHC 1 cut(s) 391
MluCI AATT 8 cut(s) 91, 258, 765, 930, 971, 979, 1296, 1432
MlyI GAGTC 1 cut(s) 371
MmeI TCCRAC 2 cut(s) 180, 927
MroI TCCGGA 1 cut(s) 411
MroXI GAANNNNTTC 1 cut(s) 1003
MseI TTAA 6 cut(s) 51, 186, 834, 1247, 1260, 1299
MslI CAYNNNNRTG 2 cut(s) 818, 1275
MspA1I CMGCKG 2 cut(s) 827, 1346
MspI CCGG 4 cut(s) 319, 412, 424, 1325
MspR9I CCNGG 2 cut(s) 425, 1325
MunI CAATTG 2 cut(s) 258, 971
Mva1269I GAATGC 1 cut(s) 622
MvnI CGCG 1 cut(s) 651
MwoI GCNNNNNNNGC 2 cut(s) 824, 1151
NciI CCSGG 2 cut(s) 425, 1325
NcoI CCATGG 1 cut(s) 441
NdeII GATC 3 cut(s) 676, 949, 1485
NlaIII CATG 5 cut(s) 445, 571, 1028, 1242, 1358
NlaIV GGNNCC 4 cut(s) 59, 288, 410, 1124
NmeAIII GCCGAG 1 cut(s) 582
NmuCI GTSAC 3 cut(s) 170, 211, 218
PagI TCATGA 1 cut(s) 1024
PctI GAATGC 1 cut(s) 622
PdmI GAANNNNTTC 1 cut(s) 1003
PfeI GAWTC 6 cut(s) 749, 779, 911, 988, 1372, 1442
PfoI TCCNGGA 1 cut(s) 1323
PinAI ACCGGT 1 cut(s) 318
PkrI GCNGC 3 cut(s) 1211, 1285, 1345
PleI GAGTC 1 cut(s) 371
PpsI GAGTC 1 cut(s) 371
PpuMI RGGWCCY 1 cut(s) 1123
Psp5II RGGWCCY 1 cut(s) 1123
PspFI CCCAGC 1 cut(s) 890
PspN4I GGNNCC 4 cut(s) 59, 288, 410, 1124
PspPI GGNCC 4 cut(s) 16, 202, 698, 1123
PspPPI RGGWCCY 1 cut(s) 1123
PstI CTGCAG 1 cut(s) 1288
PsuI RGATCY 1 cut(s) 949
PvuII CAGCTG 2 cut(s) 827, 1346
RsaI GTAC 2 cut(s) 59, 1387
RsaNI GTAC 2 cut(s) 58, 1386
RseI CAYNNNNRTG 2 cut(s) 818, 1275
SaqAI TTAA 6 cut(s) 51, 186, 834, 1247, 1260, 1299
SatI GCNGC 3 cut(s) 1210, 1284, 1344
Sau3AI GATC 3 cut(s) 676, 949, 1485
Sau96I GGNCC 4 cut(s) 16, 202, 698, 1123
SchI GAGTC 1 cut(s) 371
ScrFI CCNGG 2 cut(s) 425, 1325
SduI GDGCHC 1 cut(s) 391
SfaNI GCATC 2 cut(s) 1270, 1417
SfcI CTRYAG 4 cut(s) 338, 742, 1284, 1389
SinI GGWCC 3 cut(s) 202, 698, 1123
SmiMI CAYNNNNRTG 2 cut(s) 818, 1275
SmlI CTYRAG 3 cut(s) 302, 392, 1067
SmoI CTYRAG 3 cut(s) 302, 392, 1067
SpeI ACTAGT 1 cut(s) 215
Sse9I AATT 8 cut(s) 91, 258, 765, 930, 971, 979, 1296, 1432
SsiI CCGC 4 cut(s) 69, 620, 651, 1159
SspMI CTAG 6 cut(s) 102, 216, 896, 956, 1194, 1501
StyD4I CCNGG 2 cut(s) 423, 1323
StyI CCWWGG 1 cut(s) 441
TaaI ACNGT 8 cut(s) 57, 62, 737, 1083, 1294, 1380, 1390, 1498
TaiI ACGT 2 cut(s) 41, 1063
TaqI TCGA 4 cut(s) 158, 429, 458, 679
TasI AATT 8 cut(s) 91, 258, 765, 930, 971, 979, 1296, 1432
TfiI GAWTC 6 cut(s) 749, 779, 911, 988, 1372, 1442
Tru1I TTAA 6 cut(s) 51, 186, 834, 1247, 1260, 1299
Tru9I TTAA 6 cut(s) 51, 186, 834, 1247, 1260, 1299
TscAI CASTG 1 cut(s) 759
TseFI GTSAC 3 cut(s) 170, 211, 218
TseI GCWGC 3 cut(s) 1209, 1283, 1343
Tsp45I GTSAC 3 cut(s) 170, 211, 218
TspDTI ATGAA 3 cut(s) 326, 1013, 1109
TspGWI ACGGA 1 cut(s) 1378
TspRI CASTG 1 cut(s) 759
VneI GTGCAC 1 cut(s) 387
VpaK11BI GGWCC 3 cut(s) 202, 698, 1123
XapI RAATTY 1 cut(s) 91
XcmI CCANNNNNNNNNTGG 1 cut(s) 448
XmnI GAANNNNTTC 1 cut(s) 1003
XspI CTAG 6 cut(s) 102, 216, 896, 956, 1194, 1501
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.