Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000006

Orthogroup ID

OG0017748

Physical Location & Seq

Genomic Coordinates Forward (+)

2783151 .. 2785235

Gene Structure 2085 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug06G0022000.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1659 bp

ATGGCATTAGCGGCGAAAGGCGAGGGTCCAGCTATCGGCATCGATCTCGGAACAACTTACTCTTGCGTTGCCGTTTGGCAACACGATCGCGTTGAGATCATAGCCAATGATCAGGGCAACAGAACGACACCATCGTATGTTGCTTTTACCAATATTGGCCGTTTGATCGGTGATGCTGCCAAGAACCAAGCCACCATCAACCCTACCAATACTGTTTTTGATGCAAAGCGATTGATTGGCAGGAAATTTAGTGATGCATGTGTTAAGAGTGATATGAAATTATGGCCTTTCAAGATTCGTGGGGTGGGTGACAAGCCTATGATTGTGATCAACTACAAGAATGAAAAGAAACAATTTGCCGCTGAGGAGATATCTTCAATGGTTCTCAATAACATGCGTGAAATTGCAGAGGCCTATCTTGGACTTACAATAATGAATGTTGTTATAACTGTACCAGCATACTTCAATGACTCTCAGCGACAGGCTACAAAAGATGCTGGGGTGATTGCAGGCATGAATGTGATGCGTATTCTTAATGAGCCAACAGCCGCAGCTATTGCATATGGTCTTGACAAAAAGGCTACGGCTATTGGTGGTAAAAATGTTCTCATTTTCGATCTTGGTGGTGGTACTTTTGATGTTTCCCTTCTTAAAATTGAAGATGAGATTTTTGAGGTAAAAGCTACTGCTGGAGATACACATCTTGGTGGTGAAGATTTTGACAATAGAATGGTGCACCATTTTGTGCAAGAGTTCGAGAAGAAACACTGGAAGGACATTAGCAATGACCCCAAAGCACTGAGAAGATTGAGAACATCTTGTGAGAGAGCGAAAAGGACTCTTTCTTCAACTGCTGAAACCACCATTGAGATTGATTCTTTATTCGAGGGTATCGACTTCCACTCTAAAATAACAAGAGCAAAATTTGAAGAGCTCAACATGGATCTCTTTAGAAAATGTATGGAGACTGTGGAAAATTGTGTGAGGGATGCTAAAATTGATAGGGAAATTGTTGATGATATTGTTCTTGTTGGTGGATCTACTAGAATTCCCAAGGTACAACAGCTATTGCAAGATCTCTTTAATGGTAAGGAGCTTTGCAAGAGCATCAATCCTGATGAAGCAGTTGCATATGGTGCTGCCGTACAAGCTGCAGTTTTGCGTGGTGGAGTTGAGAAAGTGCAGGACTTACTCTTGTTAGATGTAACTCCACTTTCCCTTGGTCGTATGCTTGAGACAGGAGAGTTTGATGTTTTTATCCCAAGAAACACTACCATTCCCACCAAAAAAATTAAAATGGCCACAACTATCGTACAAAACCAAATCGGTTTGATAGTTGATGTGTATGAGGGCGAAGAAACAAGAGCCGAGAAAAACAAATCGTTGGGGCAATTTGTGCTCGAAGGAATCCCTCCTGGTCCAAAAGGAAAACCTCAAATCACAATTTGCTTCGAGATCGATGCCAATGGAGTTTTGATTGTCTCCGCTGATGAAAAACGACTGCATCTTAAGAAAAAGATTACGTTCTCCAATTATAGAGGCGGCTTATCTAAAGTGGAAATTGAGAAGATGATGAAGGAAGCAGAGAAGTACAAGTTAGAGGACCAGGAATATAACGAGAAAGTGAAGGCCAAGTCTGCATTGGCAGACTGCATATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006139 GO:0006351 GO:0006354 GO:0006355 GO:0006725 GO:0006807 GO:0008023 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009889 GO:0009987 GO:0010467 GO:0010468 GO:0010556 GO:0016070 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0031323 GO:0031326 GO:0031974 GO:0031981 GO:0032774 GO:0032784 GO:0032991 GO:0034641 GO:0034645 GO:0034654 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0046483 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0070013 GO:0071704 GO:0080090 GO:0090304 GO:0097659 GO:1901360 GO:1901362 GO:1901576 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Med26 TFIIS_C TFIIS_M

Protein Analysis

552

Amino Acids

61.19

Weight (kDa)

6.28

Isoelectric Point (pI)

25.08

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HSP70	PF00012	11 - 549	7.1e-219	Hsp70 protein
MreB_Mbl	PF06723	127 - 381	5.7e-14	MreB/Mbl protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0017748)

Species	Orthologous Gene IDs
prunus_persica	Prupe.3G238700_v2.0.a1 Prupe.6G357800_v2.0.a1
rosa_chinensis	RchiOBHm_Chr1g0335081 RchiOBHm_Chr7g0224331
rosa_laevigata	RLG00000022526
rosa_multiflora	Rmu_sc0040399.1_g000001
rosa_rugosa	Rorug05G0380500 Rorug06G0022000 Rorug07G0325000
rosa_samantha	Rh3CG362000 Rh3DG364900 Rh7DG366200

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	446
AccII	CGCG	1 cut(s)	90
AciI	CCGC	5 cut(s)	11, 360, 549, 1485, 1542
AclWI	GGATC	2 cut(s)	951, 1045
AcoI	YGGCCR	2 cut(s)	157, 1299
AcsI	RAATTY	3 cut(s)	245, 923, 1047
AfaI	GTAC	6 cut(s)	453, 631, 1059, 1146, 1314, 1592
AfiI	CCNNNNNNNGG	1 cut(s)	35
AflII	CTTAAG	1 cut(s)	1508
AgsI	TTSAA	6 cut(s)	292, 378, 466, 659, 849, 929
AjnI	CCWGG	2 cut(s)	1414, 1605
AluBI	AGCT	7 cut(s)	32, 554, 683, 934, 1066, 1096, 1151
AluI	AGCT	7 cut(s)	32, 554, 683, 934, 1066, 1096, 1151
Alw21I	GWGCWC	3 cut(s)	738, 936, 1401
Alw26I	GTCTC	3 cut(s)	959, 1229, 1486
Alw44I	GTGCAC	1 cut(s)	734
AlwI	GGATC	2 cut(s)	951, 1045
AoxI	GGCC	5 cut(s)	157, 284, 411, 1299, 1629
ApaLI	GTGCAC	1 cut(s)	734
ApeKI	GCWGC	4 cut(s)	176, 551, 1139, 1151
ApoI	RAATTY	3 cut(s)	245, 923, 1047
AspS9I	GGNCC	3 cut(s)	26, 1418, 1603
AsuHPI	GGTGA	4 cut(s)	182, 320, 514, 722
AvaII	GGWCC	3 cut(s)	26, 1418, 1603
BaeGI	GKGCMC	1 cut(s)	738
BalI	TGGCCA	1 cut(s)	1301
BanII	GRGCYC	1 cut(s)	936
Bbv12I	GWGCWC	3 cut(s)	738, 936, 1401
BbvCI	CCTCAGC	1 cut(s)	363
BbvI	GCAGC	4 cut(s)	163, 563, 1126, 1138
BccI	CCATC	2 cut(s)	139, 203
BceAI	ACGGC	4 cut(s)	56, 144, 600, 1127
BcgI	CGANNNNNNTGC	6 cut(s)	28, 62, 68, 102, 1442, 1476
BciT130I	CCWGG	2 cut(s)	1416, 1607
BclI	TGATCA	2 cut(s)	109, 327
BcoDI	GTCTC	3 cut(s)	959, 1229, 1486
BfaI	CTAG	1 cut(s)	1044
BfmI	CTRYAG	1 cut(s)	1152
BfrI	CTTAAG	1 cut(s)	1508
BglII	AGATCT	1 cut(s)	1075
BisI	GCNGC	8 cut(s)	12, 177, 360, 549, 552, 1140, 1152, 1543
BlsI	GCNGC	8 cut(s)	13, 178, 361, 550, 553, 1141, 1153, 1544
Bme1390I	CCNGG	2 cut(s)	1416, 1607
Bme18I	GGWCC	3 cut(s)	26, 1418, 1603
BmgT120I	GGNCC	3 cut(s)	26, 1418, 1603
BmiI	GGNNCC	1 cut(s)	27
BmrFI	CCNGG	2 cut(s)	1416, 1607
BpmI	CTGGAG	1 cut(s)	711
Bpu10I	CCTNAGC	1 cut(s)	363
BpuEI	CTTGAG	1 cut(s)	1253
Bsa29I	ATCGAT	2 cut(s)	42, 1458
BsaBI	GATNNNNATC	2 cut(s)	326, 699
BsaJI	CCNNGG	2 cut(s)	1053, 1219
Bsc4I	CCNNNNNNNGG	1 cut(s)	35
Bse1I	ACTGG	1 cut(s)	773
Bse3DI	GCAATG	1 cut(s)	790
Bse8I	GATNNNNATC	2 cut(s)	326, 699
BseBI	CCWGG	2 cut(s)	1416, 1607
BseCI	ATCGAT	2 cut(s)	42, 1458
BseDI	CCNNGG	2 cut(s)	1053, 1219
BseGI	GGATG	1 cut(s)	994
BseJI	GATNNNNATC	2 cut(s)	326, 699
BseLI	CCNNNNNNNGG	1 cut(s)	35
BseMI	GCAATG	1 cut(s)	790
BseMII	CTCAG	3 cut(s)	354, 488, 791
BseNI	ACTGG	1 cut(s)	773
BseRI	GAGGAG	1 cut(s)	380
BseSI	GKGCMC	1 cut(s)	738
BseXI	GCAGC	4 cut(s)	163, 563, 1126, 1138
BseYI	CCCAGC	1 cut(s)	497
BsgI	GTGCAG	1 cut(s)	1202
Bsh1236I	CGCG	1 cut(s)	90
Bsh1285I	CGRYCG	1 cut(s)	88
BshFI	GGCC	5 cut(s)	159, 286, 413, 1301, 1631
BshVI	ATCGAT	2 cut(s)	42, 1458
BsiEI	CGRYCG	1 cut(s)	88
BsiHKAI	GWGCWC	3 cut(s)	738, 936, 1401
BslI	CCNNNNNNNGG	1 cut(s)	35
BsmAI	GTCTC	3 cut(s)	959, 1229, 1486
BsnI	GGCC	5 cut(s)	159, 286, 413, 1301, 1631
Bsp1286I	GDGCHC	3 cut(s)	738, 936, 1401
BspACI	CCGC	5 cut(s)	11, 360, 549, 1485, 1542
BspANI	GGCC	5 cut(s)	159, 286, 413, 1301, 1631
BspCNI	CTCAG	3 cut(s)	355, 487, 792
BspDI	ATCGAT	2 cut(s)	42, 1458
BspFNI	CGCG	1 cut(s)	90
BspLI	GGNNCC	1 cut(s)	27
BspMAI	CTGCAG	1 cut(s)	1156
BspPI	GGATC	2 cut(s)	951, 1045
BspQI	GCTCTTC	1 cut(s)	924
BspTI	CTTAAG	1 cut(s)	1508
BsrDI	GCAATG	1 cut(s)	790
BsrI	ACTGG	1 cut(s)	773
BssECI	CCNNGG	2 cut(s)	1053, 1219
BssT1I	CCWWGG	2 cut(s)	1053, 1219
Bst2UI	CCWGG	2 cut(s)	1416, 1607
Bst4CI	ACNGT	3 cut(s)	214, 451, 970
Bst6I	CTCTTC	1 cut(s)	924
BstAFI	CTTAAG	1 cut(s)	1508
BstAPI	GCANNNNNTGC	3 cut(s)	557, 1136, 1396
BstC8I	GCNNGC	1 cut(s)	511
BstDEI	CTNAG	3 cut(s)	363, 474, 800
BstF5I	GGATG	1 cut(s)	994
BstFNI	CGCG	1 cut(s)	90
BstMAI	GTCTC	3 cut(s)	959, 1229, 1486
BstMCI	CGRYCG	1 cut(s)	88
BstMWI	GCNNNNNNNGC	6 cut(s)	11, 557, 1136, 1148, 1396, 1637
BstNI	CCWGG	2 cut(s)	1416, 1607
BstNSI	RCATGY	2 cut(s)	261, 397
BstSCI	CCNGG	2 cut(s)	1414, 1605
BstSFI	CTRYAG	1 cut(s)	1152
BstSLI	GKGCMC	1 cut(s)	738
BstUI	CGCG	1 cut(s)	90
BstV1I	GCAGC	4 cut(s)	163, 563, 1126, 1138
BstX2I	RGATCY	3 cut(s)	943, 1037, 1075
BstYI	RGATCY	3 cut(s)	943, 1037, 1075
Bsu15I	ATCGAT	2 cut(s)	42, 1458
BsuRI	GGCC	5 cut(s)	159, 286, 413, 1301, 1631
BsuTUI	ATCGAT	2 cut(s)	42, 1458
BtsCI	GGATG	1 cut(s)	994
BtsIMutI	CAGTG	2 cut(s)	766, 797
Cac8I	GCNNGC	1 cut(s)	511
Cfr13I	GGNCC	3 cut(s)	26, 1418, 1603
ClaI	ATCGAT	2 cut(s)	42, 1458
Csp6I	GTAC	6 cut(s)	452, 630, 1058, 1145, 1313, 1591
CspCI	CAANNNNNGTGG	2 cut(s)	280, 315
CviAII	CATG	4 cut(s)	258, 394, 514, 940
CviQI	GTAC	6 cut(s)	452, 630, 1058, 1145, 1313, 1591
DdeI	CTNAG	3 cut(s)	363, 474, 800
EaeI	YGGCCR	2 cut(s)	157, 1299
Eam1104I	CTCTTC	1 cut(s)	924
EarI	CTCTTC	1 cut(s)	924
Ecl136II	GAGCTC	1 cut(s)	934
Eco130I	CCWWGG	2 cut(s)	1053, 1219
Eco147I	AGGCCT	1 cut(s)	413
Eco24I	GRGCYC	1 cut(s)	936
Eco32I	GATATC	1 cut(s)	372
Eco47I	GGWCC	3 cut(s)	26, 1418, 1603
Eco53kI	GAGCTC	1 cut(s)	934
EcoICRI	GAGCTC	1 cut(s)	934
EcoRI	GAATTC	1 cut(s)	1047
EcoRII	CCWGG	2 cut(s)	1414, 1605
EcoRV	GATATC	1 cut(s)	372
EcoT14I	CCWWGG	2 cut(s)	1053, 1219
EcoT22I	ATGCAT	1 cut(s)	259
EcoT38I	GRGCYC	1 cut(s)	936
ErhI	CCWWGG	2 cut(s)	1053, 1219
FaeI	CATG	4 cut(s)	261, 397, 517, 943
FatI	CATG	4 cut(s)	257, 393, 513, 939
FauNDI	CATATG	2 cut(s)	562, 1132
FbaI	TGATCA	2 cut(s)	109, 327
Fnu4HI	GCNGC	8 cut(s)	12, 177, 360, 549, 552, 1140, 1152, 1543
FokI	GGATG	1 cut(s)	1001
FriOI	GRGCYC	1 cut(s)	936
Fsp4HI	GCNGC	8 cut(s)	12, 177, 360, 549, 552, 1140, 1152, 1543
FspBI	CTAG	1 cut(s)	1044
GluI	GCNGC	8 cut(s)	12, 177, 360, 549, 552, 1140, 1152, 1543
GsaI	CCCAGC	1 cut(s)	501
GsuI	CTGGAG	1 cut(s)	711
HaeIII	GGCC	5 cut(s)	159, 286, 413, 1301, 1631
Hin1II	CATG	4 cut(s)	261, 397, 517, 943
HinfI	GANTC	5 cut(s)	295, 470, 838, 875, 1407
HphI	GGTGA	4 cut(s)	182, 320, 514, 722
Hpy166II	GTNNAC	1 cut(s)	736
Hpy188I	TCNGA	1 cut(s)	50
Hpy188III	TCNNGA	5 cut(s)	292, 569, 757, 1115, 1453
Hpy8I	GTNNAC	1 cut(s)	736
HpyAV	CCTTC	5 cut(s)	656, 766, 1397, 1570, 1621
HpyCH4III	ACNGT	3 cut(s)	214, 451, 970
HpyCH4IV	ACGT	1 cut(s)	1523
HpyF10VI	GCNNNNNNNGC	6 cut(s)	11, 557, 1136, 1148, 1396, 1637
HpyF3I	CTNAG	3 cut(s)	363, 474, 800
HpySE526I	ACGT	1 cut(s)	1523
Hsp92II	CATG	4 cut(s)	261, 397, 517, 943
Ksp22I	TGATCA	2 cut(s)	109, 327
LguI	GCTCTTC	1 cut(s)	924
LmnI	GCTCC	1 cut(s)	1093
Lsp1109I	GCAGC	4 cut(s)	163, 563, 1126, 1138
MaeI	CTAG	1 cut(s)	1044
MaeII	ACGT	1 cut(s)	1523
MaeIII	GTNAC	2 cut(s)	308, 1204
MboII	GAAGA	9 cut(s)	366, 671, 725, 772, 816, 837, 941, 1367, 1579
MflI	RGATCY	3 cut(s)	943, 1037, 1075
MhlI	GDGCHC	3 cut(s)	738, 936, 1401
MlsI	TGGCCA	1 cut(s)	1301
MluNI	TGGCCA	1 cut(s)	1301
MlyI	GAGTC	2 cut(s)	464, 832
Mox20I	TGGCCA	1 cut(s)	1301
Mph1103I	ATGCAT	1 cut(s)	259
MscI	TGGCCA	1 cut(s)	1301
MseI	TTAA	6 cut(s)	264, 534, 651, 1083, 1293, 1509
MslI	CAYNNNNRTG	2 cut(s)	518, 705
Msp20I	TGGCCA	1 cut(s)	1301
MspA1I	CMGCKG	2 cut(s)	362, 1487
MspCI	CTTAAG	1 cut(s)	1508
MspR9I	CCNGG	2 cut(s)	1416, 1607
MvaI	CCWGG	2 cut(s)	1416, 1607
MvnI	CGCG	1 cut(s)	90
MwoI	GCNNNNNNNGC	6 cut(s)	11, 557, 1136, 1148, 1396, 1637
NdeI	CATATG	2 cut(s)	562, 1132
NlaIII	CATG	4 cut(s)	261, 397, 517, 943
NlaIV	GGNNCC	1 cut(s)	27
NmeAIII	GCCGAG	1 cut(s)	1393
NmuCI	GTSAC	1 cut(s)	308
NsiI	ATGCAT	1 cut(s)	259
NspI	RCATGY	2 cut(s)	261, 397
PceI	AGGCCT	1 cut(s)	413
PciSI	GCTCTTC	1 cut(s)	924
PcsI	WCGNNNNNNNCGW	2 cut(s)	131, 891
PfeI	GAWTC	3 cut(s)	295, 875, 1407
PkrI	GCNGC	8 cut(s)	13, 178, 361, 550, 553, 1141, 1153, 1544
Ple19I	CGATCG	1 cut(s)	88
PleI	GAGTC	2 cut(s)	464, 832
PpsI	GAGTC	2 cut(s)	464, 832
PsiI	TTATAA	1 cut(s)	446
Psp124BI	GAGCTC	1 cut(s)	936
Psp6I	CCWGG	2 cut(s)	1414, 1605
PspFI	CCCAGC	1 cut(s)	497
PspGI	CCWGG	2 cut(s)	1414, 1605
PspN4I	GGNNCC	1 cut(s)	27
PspPI	GGNCC	3 cut(s)	26, 1418, 1603
PstI	CTGCAG	1 cut(s)	1156
PsuI	RGATCY	3 cut(s)	943, 1037, 1075
PvuI	CGATCG	1 cut(s)	88
RsaI	GTAC	6 cut(s)	453, 631, 1059, 1146, 1314, 1592
RsaNI	GTAC	6 cut(s)	452, 630, 1058, 1145, 1313, 1591
RseI	CAYNNNNRTG	2 cut(s)	518, 705
SacI	GAGCTC	1 cut(s)	936
SapI	GCTCTTC	1 cut(s)	924
SaqAI	TTAA	6 cut(s)	264, 534, 651, 1083, 1293, 1509
SatI	GCNGC	8 cut(s)	12, 177, 360, 549, 552, 1140, 1152, 1543
Sau96I	GGNCC	3 cut(s)	26, 1418, 1603
SchI	GAGTC	2 cut(s)	464, 832
ScrFI	CCNGG	2 cut(s)	1416, 1607
SduI	GDGCHC	3 cut(s)	738, 936, 1401
SfcI	CTRYAG	1 cut(s)	1152
SinI	GGWCC	3 cut(s)	26, 1418, 1603
SmiMI	CAYNNNNRTG	2 cut(s)	518, 705
SmlI	CTYRAG	2 cut(s)	1232, 1508
SmoI	CTYRAG	2 cut(s)	1232, 1508
SseBI	AGGCCT	1 cut(s)	413
SsiI	CCGC	5 cut(s)	11, 360, 549, 1485, 1542
SspI	AATATT	1 cut(s)	154
SspMI	CTAG	1 cut(s)	1044
SstI	GAGCTC	1 cut(s)	936
StuI	AGGCCT	1 cut(s)	413
StyD4I	CCNGG	2 cut(s)	1414, 1605
StyI	CCWWGG	2 cut(s)	1053, 1219
TaaI	ACNGT	3 cut(s)	214, 451, 970
TaiI	ACGT	1 cut(s)	1526
TaqI	TCGA	8 cut(s)	42, 615, 756, 885, 894, 1401, 1452, 1458
TatI	WGTACW	1 cut(s)	1590
TauI	GCSGC	4 cut(s)	14, 362, 551, 1545
TfiI	GAWTC	3 cut(s)	295, 875, 1407
Tru1I	TTAA	6 cut(s)	264, 534, 651, 1083, 1293, 1509
Tru9I	TTAA	6 cut(s)	264, 534, 651, 1083, 1293, 1509
TscAI	CASTG	2 cut(s)	773, 804
TseFI	GTSAC	1 cut(s)	308
TseI	GCWGC	4 cut(s)	176, 551, 1139, 1151
Tsp45I	GTSAC	1 cut(s)	308
TspDTI	ATGAA	7 cut(s)	290, 357, 449, 530, 1134, 1506, 1589
TspRI	CASTG	2 cut(s)	773, 804
Vha464I	CTTAAG	1 cut(s)	1508
VneI	GTGCAC	1 cut(s)	734
VpaK11BI	GGWCC	3 cut(s)	26, 1418, 1603
XapI	RAATTY	3 cut(s)	245, 923, 1047
XceI	RCATGY	2 cut(s)	261, 397
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1639
XspI	CTAG	1 cut(s)	1044
Zsp2I	ATGCAT	1 cut(s)	259

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.