Rorug06G0250500

Kynurenine--oxoglutarate transaminase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0001012
Physical Location & Seq
Forward (+)
39634753 .. 39656423
21671 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0250500.1

Sequence Viewer

Length: 1074 bp
ATGGCTTTCTCTTCATTTTCCAAAGTGTCCCTTCTGCTCTTCATTGTGCCTTTCTTTTTAGGCACTTATTGTCTGGCTCATGATTTTTCAATTGTGGGTTACTCACCCGAGCACTTGTCTTCCATGGACAAGTTCATTGAGCTCTTCGAGTCATGGATATCGAAACATGGCAAGATTTACCACAACATGGAGGAGAAGCTCCATAGGTTTGAGATATTCAAAGACAATGTCAAGCATATTGATGGAAGGAATAAGAAGCTTGATGTTGACAGCTACTGGCTTGGATTGAATGAGTTTGCTGACTTGAGCCATGAAGAGTTCAAGAGCAAGTACTTGGGCCTGAAAGGTGAATCCCCAAGAAGGAGAGACTCCTCTGAAGAAGAGTTCAGTTACAGTGATTTCGTCGAAGAGGCATTGCCCAAGTCTGTGGACTGGAGAAATAAAGGAGCTGTTACTCCAGTGAAGAACCAAGGTTCATGTGGTAGCTGTTGGGCATTTTCTACAGTTGCTGCTGTTGAAGGCATAAACCAGATAGTCACAGGAAACCTAATATCGCTGTCCGAACAGCAACTGATAGATTGTGACAATTCATTCAACGATGGCTGCAATGGCGGTTTGATGGACTATGCTTTTCAATTTATCGTCTCTAATGGTGGGCTTCACAAGGAGGAAGAATACCCCTATATCATGGACGAAGGAACTTGTGAGGAATCAAGGGGACAATCAGAGGTGGTGACCATATCTGGTTATCAAGATGTGCCTCGAAATAGCGACCAAGCTCTCCTCAAGGCACTGGCTCACCAGCCCATTAGTGTTGCTATTGACGCCTCTGACAGAGATTTCCAGTTCTACAGTGGGGGTGTGTTCAACGGGCGATGTGGAACTGAGCTAGATCATGGTGTAGCAGCAGTCGGCTATGGAACATCAAAGGGGTTGGATTACATCATTGTGAAGAACTCATGGGGACCAAAGTGGGGAGAGAAGGGATACATAAGGATGAAGAGAAACACAGGAAAGCCAGAAGGAATCTGCGGTATCAACAAAATGGCTTCATATCCCACCAAGAAGAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0005488 GO:0008144 GO:0008483 GO:0010326 GO:0016740 GO:0016769 GO:0019842 GO:0030170 GO:0036094 GO:0043167 GO:0043168 GO:0048037 GO:0050662 GO:0070279 GO:0097159 GO:1901363
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Aminotran_1_2

Protein Analysis

357

Amino Acids

40.01

Weight (kDa)

5.54

Isoelectric Point (pI)

36.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I29 PF08246 49 - 107 2.5e-18 Cathepsin propeptide inhibitor domain (I29)
Peptidase_C1 PF00112 139 - 354 9.6e-85 Papain family cysteine protease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 187
AciI CCGC 2 cut(s) 612, 1032
AcuI CTGAAG 1 cut(s) 396
AcyI GRCGYC 1 cut(s) 825
AfaI GTAC 1 cut(s) 332
AfiI CCNNNNNNNGG 3 cut(s) 187, 360, 974
AgsI TTSAA 8 cut(s) 90, 220, 289, 322, 518, 595, 635, 868
AluBI AGCT 8 cut(s) 142, 199, 259, 273, 449, 486, 779, 889
AluI AGCT 8 cut(s) 142, 199, 259, 273, 449, 486, 779, 889
Alw21I GWGCWC 2 cut(s) 114, 144
Alw26I GTCTC 2 cut(s) 360, 649
AlwNI CAGNNNCTG 3 cut(s) 276, 509, 571
Ama87I CYCGRG 1 cut(s) 107
AoxI GGCC 1 cut(s) 337
ApeKI GCWGC 3 cut(s) 509, 603, 905
ArsI GACNNNNNNTTYG 2 cut(s) 213, 245
AspS9I GGNCC 2 cut(s) 337, 965
AsuHPI GGTGA 4 cut(s) 96, 359, 745, 791
AvaI CYCGRG 1 cut(s) 107
AvaII GGWCC 1 cut(s) 965
BanII GRGCYC 1 cut(s) 144
BbsI GAAGAC 1 cut(s) 111
Bbv12I GWGCWC 2 cut(s) 114, 144
BbvI GCAGC 3 cut(s) 496, 590, 917
BccI CCATC 3 cut(s) 236, 593, 613
BciVI GTATCC 1 cut(s) 980
BcoDI GTCTC 2 cut(s) 360, 649
BfaI CTAG 1 cut(s) 890
BfmI CTRYAG 2 cut(s) 501, 850
BfuI GTATCC 1 cut(s) 980
BisI GCNGC 3 cut(s) 510, 604, 906
BlsI GCNGC 3 cut(s) 511, 605, 907
BmcAI AGTACT 1 cut(s) 332
Bme18I GGWCC 1 cut(s) 965
BmeT110I CYCGRG 1 cut(s) 107
BmgT120I GGNCC 2 cut(s) 337, 965
BmiI GGNNCC 1 cut(s) 966
BpiI GAAGAC 1 cut(s) 111
BpmI CTGGAG 2 cut(s) 441, 454
BpuEI CTTGAG 2 cut(s) 325, 770
BsaHI GRCGYC 1 cut(s) 825
BsaJI CCNNGG 2 cut(s) 123, 469
Bsc4I CCNNNNNNNGG 3 cut(s) 187, 360, 974
Bse1I ACTGG 5 cut(s) 281, 437, 458, 798, 844
Bse3DI GCAATG 2 cut(s) 413, 613
BseDI CCNNGG 2 cut(s) 123, 469
BseGI GGATG 1 cut(s) 1002
BseLI CCNNNNNNNGG 3 cut(s) 187, 360, 974
BseMI GCAATG 2 cut(s) 413, 613
BseMII CTCAG 1 cut(s) 876
BseNI ACTGG 5 cut(s) 281, 437, 458, 798, 844
BseRI GAGGAG 3 cut(s) 206, 361, 773
BseXI GCAGC 3 cut(s) 496, 590, 917
BshFI GGCC 1 cut(s) 339
BsiHKAI GWGCWC 2 cut(s) 114, 144
BsiHKCI CYCGRG 1 cut(s) 107
BslFI GGGAC 3 cut(s) 13, 732, 978
BslI CCNNNNNNNGG 3 cut(s) 187, 360, 974
BsmAI GTCTC 2 cut(s) 360, 649
BsmBI CGTCTC 1 cut(s) 649
BsmFI GGGAC 3 cut(s) 13, 732, 978
BsnI GGCC 1 cut(s) 339
BsoBI CYCGRG 1 cut(s) 107
Bsp1286I GDGCHC 2 cut(s) 114, 144
Bsp143I GATC 1 cut(s) 892
Bsp19I CCATGG 1 cut(s) 123
BspACI CCGC 2 cut(s) 612, 1032
BspANI GGCC 1 cut(s) 339
BspCNI CTCAG 1 cut(s) 877
BspHI TCATGA 1 cut(s) 79
BspLI GGNNCC 1 cut(s) 966
BspQI GCTCTTC 2 cut(s) 44, 149
BsrDI GCAATG 2 cut(s) 413, 613
BsrI ACTGG 5 cut(s) 281, 437, 458, 798, 844
BssECI CCNNGG 2 cut(s) 123, 469
BssMI GATC 1 cut(s) 892
BssNI GRCGYC 1 cut(s) 825
BssT1I CCWWGG 2 cut(s) 123, 469
Bst4CI ACNGT 3 cut(s) 395, 505, 854
Bst6I CTCTTC 7 cut(s) 16, 44, 149, 309, 375, 402, 995
BstACI GRCGYC 1 cut(s) 825
BstDEI CTNAG 1 cut(s) 885
BstDSI CCRYGG 1 cut(s) 123
BstEII GGTNACC 1 cut(s) 733
BstF5I GGATG 1 cut(s) 1002
BstKTI GATC 1 cut(s) 895
BstMAI GTCTC 2 cut(s) 360, 649
BstMBI GATC 1 cut(s) 892
BstMWI GCNNNNNNNGC 2 cut(s) 609, 824
BstPI GGTNACC 1 cut(s) 733
BstSFI CTRYAG 2 cut(s) 501, 850
BstV1I GCAGC 3 cut(s) 496, 590, 917
BstV2I GAAGAC 1 cut(s) 111
BstXI CCANNNNNNTGG 1 cut(s) 427
BsuI GTATCC 1 cut(s) 980
BsuRI GGCC 1 cut(s) 339
BtgI CCRYGG 1 cut(s) 123
BtgZI GCGATG 1 cut(s) 889
BtsCI GGATG 1 cut(s) 1002
BtsIMutI CAGTG 4 cut(s) 400, 465, 791, 859
CaiI CAGNNNCTG 3 cut(s) 276, 509, 571
CciI TCATGA 1 cut(s) 79
Cfr13I GGNCC 2 cut(s) 337, 965
CseI GACGC 1 cut(s) 833
Csp6I GTAC 1 cut(s) 331
CviQI GTAC 1 cut(s) 331
DdeI CTNAG 1 cut(s) 885
DpnI GATC 1 cut(s) 894
DpnII GATC 1 cut(s) 892
Eam1104I CTCTTC 7 cut(s) 16, 44, 149, 309, 375, 402, 995
EarI CTCTTC 7 cut(s) 16, 44, 149, 309, 375, 402, 995
Ecl136II GAGCTC 1 cut(s) 142
Eco130I CCWWGG 2 cut(s) 123, 469
Eco24I GRGCYC 1 cut(s) 144
Eco32I GATATC 1 cut(s) 159
Eco47I GGWCC 1 cut(s) 965
Eco53kI GAGCTC 1 cut(s) 142
Eco57I CTGAAG 1 cut(s) 396
Eco88I CYCGRG 1 cut(s) 107
Eco91I GGTNACC 1 cut(s) 733
EcoICRI GAGCTC 1 cut(s) 142
EcoO65I GGTNACC 1 cut(s) 733
EcoRV GATATC 1 cut(s) 159
EcoT14I CCWWGG 2 cut(s) 123, 469
EcoT38I GRGCYC 1 cut(s) 144
ErhI CCWWGG 2 cut(s) 123, 469
Esp3I CGTCTC 1 cut(s) 649
FalI AAGNNNNNCTT 2 cut(s) 15, 47
FaqI GGGAC 3 cut(s) 13, 732, 978
Fnu4HI GCNGC 3 cut(s) 510, 604, 906
FokI GGATG 1 cut(s) 1009
FriOI GRGCYC 1 cut(s) 144
Fsp4HI GCNGC 3 cut(s) 510, 604, 906
FspBI CTAG 1 cut(s) 890
GluI GCNGC 3 cut(s) 510, 604, 906
GsuI CTGGAG 2 cut(s) 441, 454
HaeIII GGCC 1 cut(s) 339
HgaI GACGC 1 cut(s) 833
Hin1I GRCGYC 1 cut(s) 825
HincII GTYRAC 1 cut(s) 268
HindII GTYRAC 1 cut(s) 268
HindIII AAGCTT 1 cut(s) 257
HinfI GANTC 5 cut(s) 149, 350, 368, 710, 1026
HphI GGTGA 4 cut(s) 96, 359, 745, 791
Hpy166II GTNNAC 2 cut(s) 268, 430
Hpy188I TCNGA 4 cut(s) 376, 562, 727, 832
Hpy188III TCNNGA 3 cut(s) 80, 322, 752
Hpy8I GTNNAC 2 cut(s) 268, 430
Hpy99I CGWCG 1 cut(s) 407
HpyAV CCTTC 7 cut(s) 41, 240, 354, 512, 689, 976, 1016
HpyCH4III ACNGT 3 cut(s) 395, 505, 854
HpyCH4V TGCA 1 cut(s) 606
HpyF10VI GCNNNNNNNGC 2 cut(s) 609, 824
HpyF3I CTNAG 1 cut(s) 885
Hsp92I GRCGYC 1 cut(s) 825
Kzo9I GATC 1 cut(s) 892
LguI GCTCTTC 2 cut(s) 44, 149
LmnI GCTCC 2 cut(s) 204, 446
Lsp1109I GCAGC 3 cut(s) 496, 590, 917
MaeI CTAG 1 cut(s) 890
MaeIII GTNAC 6 cut(s) 98, 389, 451, 535, 581, 733
MalI GATC 1 cut(s) 894
MboI GATC 1 cut(s) 892
MfeI CAATTG 1 cut(s) 90
MhlI GDGCHC 2 cut(s) 114, 144
MluCI AATT 3 cut(s) 90, 586, 635
MlyI GAGTC 2 cut(s) 158, 362
MmeI TCCRAC 1 cut(s) 915
MnlI CCTC 9 cut(s) 184, 382, 403, 661, 700, 721, 771, 794, 838
MslI CAYNNNNRTG 3 cut(s) 240, 947, 995
MunI CAATTG 1 cut(s) 90
MwoI GCNNNNNNNGC 2 cut(s) 609, 824
NcoI CCATGG 1 cut(s) 123
NdeII GATC 1 cut(s) 892
NlaIV GGNNCC 1 cut(s) 966
NmuCI GTSAC 3 cut(s) 535, 581, 733
PagI TCATGA 1 cut(s) 79
PciSI GCTCTTC 2 cut(s) 44, 149
PfeI GAWTC 3 cut(s) 350, 710, 1026
PflFI GACNNNGTC 1 cut(s) 227
PflMI CCANNNNNTGG 1 cut(s) 187
PkrI GCNGC 3 cut(s) 511, 605, 907
PleI GAGTC 2 cut(s) 157, 362
PpsI GAGTC 2 cut(s) 157, 362
Psp124BI GAGCTC 1 cut(s) 144
PspEI GGTNACC 1 cut(s) 733
PspN4I GGNNCC 1 cut(s) 966
PspPI GGNCC 2 cut(s) 337, 965
PstNI CAGNNNCTG 3 cut(s) 276, 509, 571
PsyI GACNNNGTC 1 cut(s) 227
RsaI GTAC 1 cut(s) 332
RsaNI GTAC 1 cut(s) 331
RseI CAYNNNNRTG 3 cut(s) 240, 947, 995
SacI GAGCTC 1 cut(s) 144
SapI GCTCTTC 2 cut(s) 44, 149
SatI GCNGC 3 cut(s) 510, 604, 906
Sau3AI GATC 1 cut(s) 892
Sau96I GGNCC 2 cut(s) 337, 965
ScaI AGTACT 1 cut(s) 332
SchI GAGTC 2 cut(s) 158, 362
SduI GDGCHC 2 cut(s) 114, 144
SfcI CTRYAG 2 cut(s) 501, 850
SinI GGWCC 1 cut(s) 965
SmiMI CAYNNNNRTG 3 cut(s) 240, 947, 995
SmlI CTYRAG 2 cut(s) 304, 785
SmoI CTYRAG 2 cut(s) 304, 785
Sse9I AATT 3 cut(s) 90, 586, 635
SsiI CCGC 2 cut(s) 612, 1032
SspMI CTAG 1 cut(s) 890
SstI GAGCTC 1 cut(s) 144
StyI CCWWGG 2 cut(s) 123, 469
TaaI ACNGT 3 cut(s) 395, 505, 854
TaqI TCGA 4 cut(s) 147, 161, 405, 763
TasI AATT 3 cut(s) 90, 586, 635
TatI WGTACW 1 cut(s) 330
TfiI GAWTC 3 cut(s) 350, 710, 1026
TscAI CASTG 4 cut(s) 400, 465, 798, 859
TseFI GTSAC 3 cut(s) 535, 581, 733
TseI GCWGC 3 cut(s) 509, 603, 905
Tsp45I GTSAC 3 cut(s) 535, 581, 733
TspDTI ATGAA 7 cut(s) 31, 124, 327, 465, 579, 1013, 1041
TspRI CASTG 4 cut(s) 400, 465, 798, 859
Tth111I GACNNNGTC 1 cut(s) 227
Van91I CCANNNNNTGG 1 cut(s) 187
VpaK11BI GGWCC 1 cut(s) 965
XcmI CCANNNNNNNNNTGG 2 cut(s) 476, 851
XspI CTAG 1 cut(s) 890
ZrmI AGTACT 1 cut(s) 332
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.