Rorug06G0334900

breast cancer carboxy-terminal domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0006642
Physical Location & Seq
Forward (+)
48730867 .. 48731786
920 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0334900.1

Sequence Viewer

Length: 660 bp
ATGCAGGAGAAGAGAAAACACTGGCTGCCTCTTGGCATTTGCCACAGGCTCTTCACCTTCATCATGAACAGCCTCATTGCTAGAAGGCTTGTCAAGCACGTAACGATAACCAGCCATCATCCAACGCCTCAAGGTTCAAGCAATGAAGTGGTTTTTGTGGAAGACCAACAACCCCTTGTGCCAAACAGGCCTAATAGAGTGGAGAGCATATACATTCAACAGGCTCAGCATACACCAGAAGACAATTCGGGTTTTGAGATCCAAGTTCACTACAAGCAGACAGATGATAATGATTCAAAGATGAGGAATTCTTCGGATAGCCACAAATTGTCTTCGAGTAAGAGTTTACAAGAGTTGAAGCCTATTGTCACAGGTGTATCGCAAGCTAAGGACCCGGAAAAGAGTTTAAGCATTGCAGATGCAGTGAGGTTGGAGAAAGAGAAGGACAATAAGAAGAAAGGTATAATGCTAATGAGTAATGAAAAATTTACACTAGCAATGGTGGGTGAAGAAGATGAGAGATCAAAGCTTCCTTTGAAGCGTGCAAGGCCACTTTTTTATGTTGCACCAAATATAAATGAAAAATCAGACGAATTCATTCGGAGTAGGAAGGAAGCTATGAGAAGGAGCCAAAGCCTCGAGCTGCCCAAAACATATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PTCB-BRCT

Protein Analysis

219

Amino Acids

25.26

Weight (kDa)

9.42

Isoelectric Point (pI)

62.07

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 253
AcsI RAATTY 3 cut(s) 307, 485, 593
AgsI TTSAA 5 cut(s) 138, 218, 297, 358, 538
AluBI AGCT 4 cut(s) 386, 529, 617, 643
AluI AGCT 4 cut(s) 386, 529, 617, 643
AlwI GGATC 1 cut(s) 253
Ama87I CYCGRG 1 cut(s) 638
AoxI GGCC 2 cut(s) 188, 548
ApeKI GCWGC 2 cut(s) 25, 643
ApoI RAATTY 3 cut(s) 307, 485, 593
Asp700I GAANNNNTTC 1 cut(s) 597
AspS9I GGNCC 1 cut(s) 391
AsuC2I CCSGG 1 cut(s) 395
AsuHPI GGTGA 2 cut(s) 46, 518
AvaI CYCGRG 1 cut(s) 638
AvaII GGWCC 1 cut(s) 391
BaeI ACNNNNGTAYC 2 cut(s) 360, 393
BbsI GAAGAC 3 cut(s) 168, 246, 324
BbvI GCAGC 2 cut(s) 12, 630
BccI CCATC 1 cut(s) 123
BcnI CCSGG 1 cut(s) 395
BfaI CTAG 2 cut(s) 81, 494
BglI GCCNNNNNGGC 1 cut(s) 187
BisI GCNGC 2 cut(s) 26, 644
BlpI GCTNAGC 1 cut(s) 225
BlsI GCNGC 2 cut(s) 27, 645
Bme1390I CCNGG 1 cut(s) 395
Bme18I GGWCC 1 cut(s) 391
BmeT110I CYCGRG 1 cut(s) 638
BmgT120I GGNCC 1 cut(s) 391
BmiI GGNNCC 2 cut(s) 393, 629
BmrFI CCNGG 1 cut(s) 395
BmsI GCATC 1 cut(s) 409
BpiI GAAGAC 3 cut(s) 168, 246, 324
Bpu10I CCTNAGC 1 cut(s) 387
Bpu1102I GCTNAGC 1 cut(s) 225
BpuEI CTTGAG 1 cut(s) 114
BpuMI CCSGG 1 cut(s) 395
BsaAI YACGTR 1 cut(s) 100
Bse1I ACTGG 1 cut(s) 26
Bse3DI GCAATG 4 cut(s) 75, 148, 411, 504
BseGI GGATG 1 cut(s) 118
BseMI GCAATG 4 cut(s) 75, 148, 411, 504
BseMII CTCAG 1 cut(s) 239
BseNI ACTGG 1 cut(s) 26
BseXI GCAGC 2 cut(s) 12, 630
BshFI GGCC 2 cut(s) 190, 550
BsiHKCI CYCGRG 1 cut(s) 638
BsiSI CCGG 1 cut(s) 395
BsnI GGCC 2 cut(s) 190, 550
BsoBI CYCGRG 1 cut(s) 638
Bsp143I GATC 2 cut(s) 258, 521
Bsp1720I GCTNAGC 1 cut(s) 225
BspANI GGCC 2 cut(s) 190, 550
BspCNI CTCAG 1 cut(s) 238
BspHI TCATGA 1 cut(s) 63
BspLI GGNNCC 2 cut(s) 393, 629
BspPI GGATC 1 cut(s) 253
BspQI GCTCTTC 1 cut(s) 56
BsrDI GCAATG 4 cut(s) 75, 148, 411, 504
BsrI ACTGG 1 cut(s) 26
BssMI GATC 2 cut(s) 258, 521
Bst6I CTCTTC 2 cut(s) 5, 56
BstBAI YACGTR 1 cut(s) 100
BstC8I GCNNGC 2 cut(s) 384, 543
BstDEI CTNAG 2 cut(s) 225, 387
BstF5I GGATG 1 cut(s) 118
BstKTI GATC 2 cut(s) 261, 524
BstMBI GATC 2 cut(s) 258, 521
BstMWI GCNNNNNNNGC 3 cut(s) 94, 187, 547
BstSCI CCNGG 1 cut(s) 393
BstV1I GCAGC 2 cut(s) 12, 630
BstV2I GAAGAC 3 cut(s) 168, 246, 324
BstX2I RGATCY 1 cut(s) 258
BstYI RGATCY 1 cut(s) 258
BsuRI GGCC 2 cut(s) 190, 550
BtsCI GGATG 1 cut(s) 118
BtsI GCAGTG 1 cut(s) 429
BtsIMutI CAGTG 2 cut(s) 19, 429
Cac8I GCNNGC 2 cut(s) 384, 543
CciI TCATGA 1 cut(s) 63
Cfr13I GGNCC 1 cut(s) 391
CviAII CATG 1 cut(s) 64
DdeI CTNAG 2 cut(s) 225, 387
DpnI GATC 2 cut(s) 260, 523
DpnII GATC 2 cut(s) 258, 521
Eam1104I CTCTTC 2 cut(s) 5, 56
EarI CTCTTC 2 cut(s) 5, 56
Eco147I AGGCCT 1 cut(s) 190
Eco47I GGWCC 1 cut(s) 391
Eco88I CYCGRG 1 cut(s) 638
EcoO109I RGGNCCY 1 cut(s) 391
EcoRI GAATTC 2 cut(s) 307, 593
FaeI CATG 1 cut(s) 67
FaiI YATR 9 cut(s) 65, 209, 211, 231, 464, 561, 575, 620, 655
FatI CATG 1 cut(s) 63
Fnu4HI GCNGC 2 cut(s) 26, 644
FokI GGATG 1 cut(s) 105
Fsp4HI GCNGC 2 cut(s) 26, 644
FspBI CTAG 2 cut(s) 81, 494
GluI GCNGC 2 cut(s) 26, 644
HaeIII GGCC 2 cut(s) 190, 550
HapII CCGG 1 cut(s) 395
Hin1II CATG 1 cut(s) 67
HindIII AAGCTT 1 cut(s) 527
HinfI GANTC 1 cut(s) 293
HpaII CCGG 1 cut(s) 395
HphI GGTGA 2 cut(s) 46, 518
Hpy166II GTNNAC 2 cut(s) 268, 347
Hpy188I TCNGA 3 cut(s) 316, 589, 603
Hpy188III TCNNGA 1 cut(s) 64
Hpy8I GTNNAC 2 cut(s) 268, 347
HpyAV CCTTC 5 cut(s) 67, 78, 436, 604, 618
HpyCH4IV ACGT 1 cut(s) 99
HpyCH4V TGCA 5 cut(s) 4, 416, 422, 545, 566
HpyF10VI GCNNNNNNNGC 3 cut(s) 94, 187, 547
HpyF3I CTNAG 2 cut(s) 225, 387
HpySE526I ACGT 1 cut(s) 99
Hsp92II CATG 1 cut(s) 67
Kzo9I GATC 2 cut(s) 258, 521
LguI GCTCTTC 1 cut(s) 56
LmnI GCTCC 1 cut(s) 627
LpnPI CCDG 8 cut(s) 7, 31, 124, 172, 206, 249, 357, 408
Lsp1109I GCAGC 2 cut(s) 12, 630
LweI GCATC 1 cut(s) 409
MaeI CTAG 2 cut(s) 81, 494
MaeII ACGT 1 cut(s) 99
MaeIII GTNAC 2 cut(s) 100, 367
MalI GATC 2 cut(s) 260, 523
MboI GATC 2 cut(s) 258, 521
MboII GAAGA 9 cut(s) 22, 43, 173, 251, 303, 324, 466, 521, 524
MflI RGATCY 1 cut(s) 258
MluCI AATT 5 cut(s) 244, 307, 326, 485, 593
MmeI TCCRAC 2 cut(s) 146, 411
MnlI CCTC 6 cut(s) 39, 83, 138, 297, 420, 647
MroXI GAANNNNTTC 1 cut(s) 597
MseI TTAA 1 cut(s) 407
MspI CCGG 1 cut(s) 395
MspR9I CCNGG 1 cut(s) 395
MwoI GCNNNNNNNGC 3 cut(s) 94, 187, 547
NciI CCSGG 1 cut(s) 395
NdeII GATC 2 cut(s) 258, 521
NlaIII CATG 1 cut(s) 67
NlaIV GGNNCC 2 cut(s) 393, 629
NmuCI GTSAC 1 cut(s) 367
PaeR7I CTCGAG 1 cut(s) 638
PagI TCATGA 1 cut(s) 63
PceI AGGCCT 1 cut(s) 190
PciSI GCTCTTC 1 cut(s) 56
PdmI GAANNNNTTC 1 cut(s) 597
PfeI GAWTC 1 cut(s) 293
PkrI GCNGC 2 cut(s) 27, 645
Ppu21I YACGTR 1 cut(s) 100
PpuMI RGGWCCY 1 cut(s) 391
Psp5II RGGWCCY 1 cut(s) 391
PspN4I GGNNCC 2 cut(s) 393, 629
PspPI GGNCC 1 cut(s) 391
PspPPI RGGWCCY 1 cut(s) 391
PspXI VCTCGAGB 1 cut(s) 638
PsuI RGATCY 1 cut(s) 258
SapI GCTCTTC 1 cut(s) 56
SaqAI TTAA 1 cut(s) 407
SatI GCNGC 2 cut(s) 26, 644
Sau3AI GATC 2 cut(s) 258, 521
Sau96I GGNCC 1 cut(s) 391
ScrFI CCNGG 1 cut(s) 395
SfaNI GCATC 1 cut(s) 409
Sfr274I CTCGAG 1 cut(s) 638
SinI GGWCC 1 cut(s) 391
SlaI CTCGAG 1 cut(s) 638
SmlI CTYRAG 2 cut(s) 129, 638
SmoI CTYRAG 2 cut(s) 129, 638
Sse9I AATT 5 cut(s) 244, 307, 326, 485, 593
SseBI AGGCCT 1 cut(s) 190
SspMI CTAG 2 cut(s) 81, 494
StuI AGGCCT 1 cut(s) 190
StyD4I CCNGG 1 cut(s) 393
TaiI ACGT 1 cut(s) 102
TaqI TCGA 2 cut(s) 335, 639
TasI AATT 5 cut(s) 244, 307, 326, 485, 593
TfiI GAWTC 1 cut(s) 293
Tru1I TTAA 1 cut(s) 407
Tru9I TTAA 1 cut(s) 407
TscAI CASTG 2 cut(s) 26, 429
TseFI GTSAC 1 cut(s) 367
TseI GCWGC 2 cut(s) 25, 643
Tsp45I GTSAC 1 cut(s) 367
TspDTI ATGAA 6 cut(s) 49, 80, 159, 495, 586, 594
TspRI CASTG 2 cut(s) 26, 429
VpaK11BI GGWCC 1 cut(s) 391
XapI RAATTY 3 cut(s) 307, 485, 593
XhoI CTCGAG 1 cut(s) 638
XmnI GAANNNNTTC 1 cut(s) 597
XspI CTAG 2 cut(s) 81, 494
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.