Rh1AG050000

F-actin-capping proteins bind in a Ca(2 )-independent manner to the fast growing ends of actin filaments (barbed end) thereby blocking the exchange of subunits at these ends. Unlike other capping proteins (such as gelsolin and severin), these proteins do not sever actin filaments

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0019853
Physical Location & Seq
Forward (+)
8383980 .. 8394614
10635 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG050000.1

Sequence Viewer

Length: 474 bp
ATGGCGGACGAAGAGGAATCTGAGCTAAGCGAGAAGCAGAAGATTGATATAGCCAAGTGGTTCCTCCTCAACTCTCCTCCTGGTGAAATCCAATTCGTCGCCGAAGATGTGAAGGCGGTAGTCAACGACGACGTTTTGTACGAAGAAGCAGCTTCAGAGGCTTTCCCACTGTATAACAAATCACACATGATTTCGCTAGAAATGCCTGGTGGAATTGGAGATGTTCTAGTTACATCTTTCGGTGAGCTCCGTGGGACTGAGTACCTTGATCCCAAGACTGCTCATGTTGCTGTCGTTGACCATATCAAACAGGTCTATTCAAAAAAAAAATTGGTTCAGGTTTGTACAGACGTGAGACCTGCATTGGATGAGGAACTTCCATCCGCATATGTTGAGGTGTACAGCTTTCTTGCTTCATGGATCCTTAAAGTCTCTTTTCAACCATATGGCCTCCCAAGATGTTGTACTAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
F-actin_cap_A

Protein Analysis

157

Amino Acids

17.55

Weight (kDa)

4.58

Isoelectric Point (pI)

45.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-actin_cap_A PF01267 18 - 134 9.3e-22 F-actin capping protein alpha subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes (Group: OG0019853)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr1g0322051 RchiOBHm_Chr1g0322151
rosa_multiflora Rmu_co8251333.1_g000001
rosa_samantha Rh1AG049100 Rh1AG050000 Rh3AG069300
rosa_wichuraiana Rw1G004560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 367
AciI CCGC 3 cut(s) 5, 116, 384
AclWI GGATC 3 cut(s) 263, 415, 428
AcuI CTGAAG 1 cut(s) 138
AfaI GTAC 5 cut(s) 140, 263, 346, 401, 466
AgsI TTSAA 2 cut(s) 321, 440
AhlI ACTAGT 1 cut(s) 467
AjiI CACGTC 1 cut(s) 352
AjnI CCWGG 2 cut(s) 79, 205
AluBI AGCT 4 cut(s) 25, 152, 247, 405
AluI AGCT 4 cut(s) 25, 152, 247, 405
Alw21I GWGCWC 1 cut(s) 249
Alw26I GTCTC 2 cut(s) 349, 436
AlwI GGATC 3 cut(s) 263, 415, 428
AoxI GGCC 1 cut(s) 448
ApeKI GCWGC 1 cut(s) 149
AsuHPI GGTGA 2 cut(s) 95, 254
BamHI GGATCC 1 cut(s) 420
BanII GRGCYC 1 cut(s) 249
Bbv12I GWGCWC 1 cut(s) 249
BbvI GCAGC 1 cut(s) 161
BccI CCATC 1 cut(s) 388
BciT130I CCWGG 2 cut(s) 81, 207
BcoDI GTCTC 2 cut(s) 349, 436
BcuI ACTAGT 1 cut(s) 467
BfaI CTAG 3 cut(s) 197, 227, 468
BfuAI ACCTGC 1 cut(s) 367
BisI GCNGC 1 cut(s) 150
BlpI GCTNAGC 1 cut(s) 26
BlsI GCNGC 1 cut(s) 151
Bme1390I CCNGG 2 cut(s) 81, 207
BmgBI CACGTC 1 cut(s) 352
BmiI GGNNCC 2 cut(s) 62, 422
BmrFI CCNGG 2 cut(s) 81, 207
Bpu1102I GCTNAGC 1 cut(s) 26
BsaI GGTCTC 1 cut(s) 349
BsaJI CCNNGG 1 cut(s) 250
BseBI CCWGG 2 cut(s) 81, 207
BseDI CCNNGG 1 cut(s) 250
BseGI GGATG 2 cut(s) 373, 380
BseMII CTCAG 2 cut(s) 12, 249
BseRI GAGGAG 2 cut(s) 56, 66
BseXI GCAGC 1 cut(s) 161
BshFI GGCC 1 cut(s) 450
BsiHKAI GWGCWC 1 cut(s) 249
BslFI GGGAC 1 cut(s) 268
BsmAI GTCTC 2 cut(s) 349, 436
BsmFI GGGAC 1 cut(s) 268
BsnI GGCC 1 cut(s) 450
Bso31I GGTCTC 1 cut(s) 349
Bsp1286I GDGCHC 1 cut(s) 249
Bsp1407I TGTACA 2 cut(s) 344, 399
Bsp143I GATC 2 cut(s) 268, 420
Bsp1720I GCTNAGC 1 cut(s) 26
BspACI CCGC 3 cut(s) 5, 116, 384
BspANI GGCC 1 cut(s) 450
BspCNI CTCAG 2 cut(s) 13, 250
BspLI GGNNCC 2 cut(s) 62, 422
BspMI ACCTGC 1 cut(s) 367
BspPI GGATC 3 cut(s) 263, 415, 428
BspTNI GGTCTC 1 cut(s) 349
BsrGI TGTACA 2 cut(s) 344, 399
BssECI CCNNGG 1 cut(s) 250
BssMI GATC 2 cut(s) 268, 420
Bst2UI CCWGG 2 cut(s) 81, 207
Bst4CI ACNGT 1 cut(s) 171
Bst6I CTCTTC 1 cut(s) 6
BstAUI TGTACA 2 cut(s) 344, 399
BstDEI CTNAG 3 cut(s) 21, 26, 258
BstDSI CCRYGG 1 cut(s) 250
BstF5I GGATG 2 cut(s) 373, 380
BstKTI GATC 2 cut(s) 271, 423
BstMAI GTCTC 2 cut(s) 349, 436
BstMBI GATC 2 cut(s) 268, 420
BstMWI GCNNNNNNNGC 3 cut(s) 158, 202, 287
BstNI CCWGG 2 cut(s) 81, 207
BstSCI CCNGG 2 cut(s) 79, 205
BstV1I GCAGC 1 cut(s) 161
BstX2I RGATCY 1 cut(s) 420
BstYI RGATCY 1 cut(s) 420
BsuRI GGCC 1 cut(s) 450
BtgI CCRYGG 1 cut(s) 250
BtrI CACGTC 1 cut(s) 352
BtsCI GGATG 2 cut(s) 373, 380
BtsIMutI CAGTG 1 cut(s) 167
BveI ACCTGC 1 cut(s) 367
Csp6I GTAC 5 cut(s) 139, 262, 345, 400, 465
CviAII CATG 3 cut(s) 187, 284, 417
CviJI RGCY 7 cut(s) 25, 53, 152, 161, 247, 405, 450
CviKI_1 RGCY 7 cut(s) 25, 53, 152, 161, 247, 405, 450
CviQI GTAC 5 cut(s) 139, 262, 345, 400, 465
DdeI CTNAG 3 cut(s) 21, 26, 258
DpnI GATC 2 cut(s) 270, 422
DpnII GATC 2 cut(s) 268, 420
Eam1104I CTCTTC 1 cut(s) 6
EarI CTCTTC 1 cut(s) 6
EciI GGCGGA 1 cut(s) 20
Ecl136II GAGCTC 1 cut(s) 247
Eco24I GRGCYC 1 cut(s) 249
Eco31I GGTCTC 1 cut(s) 349
Eco53kI GAGCTC 1 cut(s) 247
Eco57I CTGAAG 1 cut(s) 138
EcoICRI GAGCTC 1 cut(s) 247
EcoRII CCWGG 2 cut(s) 79, 205
EcoT38I GRGCYC 1 cut(s) 249
FaeI CATG 3 cut(s) 190, 287, 420
FaqI GGGAC 1 cut(s) 268
FatI CATG 3 cut(s) 186, 283, 416
FauNDI CATATG 2 cut(s) 388, 445
Fnu4HI GCNGC 1 cut(s) 150
FokI GGATG 2 cut(s) 367, 380
FriOI GRGCYC 1 cut(s) 249
Fsp4HI GCNGC 1 cut(s) 150
FspBI CTAG 3 cut(s) 197, 227, 468
GluI GCNGC 1 cut(s) 150
HaeIII GGCC 1 cut(s) 450
Hin1II CATG 3 cut(s) 190, 287, 420
HincII GTYRAC 2 cut(s) 124, 298
HindII GTYRAC 2 cut(s) 124, 298
HinfI GANTC 1 cut(s) 17
HphI GGTGA 2 cut(s) 95, 254
Hpy166II GTNNAC 3 cut(s) 124, 298, 400
Hpy188I TCNGA 2 cut(s) 22, 157
Hpy8I GTNNAC 3 cut(s) 124, 298, 400
Hpy99I CGWCG 3 cut(s) 101, 131, 134
HpyAV CCTTC 1 cut(s) 106
HpyCH4III ACNGT 1 cut(s) 171
HpyCH4IV ACGT 2 cut(s) 132, 351
HpyCH4V TGCA 1 cut(s) 362
HpyF10VI GCNNNNNNNGC 3 cut(s) 158, 202, 287
HpyF3I CTNAG 3 cut(s) 21, 26, 258
HpySE526I ACGT 2 cut(s) 132, 351
Hsp92II CATG 3 cut(s) 190, 287, 420
Kzo9I GATC 2 cut(s) 268, 420
LmnI GCTCC 1 cut(s) 252
LpnPI CCDG 7 cut(s) 66, 93, 192, 219, 296, 323, 372
Lsp1109I GCAGC 1 cut(s) 161
MaeI CTAG 3 cut(s) 197, 227, 468
MaeII ACGT 2 cut(s) 132, 351
MaeIII GTNAC 1 cut(s) 229
MalI GATC 2 cut(s) 270, 422
MboI GATC 2 cut(s) 268, 420
MboII GAAGA 4 cut(s) 23, 52, 116, 155
MflI RGATCY 1 cut(s) 420
MhlI GDGCHC 1 cut(s) 249
MluCI AATT 3 cut(s) 92, 213, 329
MnlI CCTC 8 cut(s) 7, 74, 77, 87, 151, 364, 388, 461
MseI TTAA 1 cut(s) 426
MspR9I CCNGG 2 cut(s) 81, 207
MvaI CCWGG 2 cut(s) 81, 207
MwoI GCNNNNNNNGC 3 cut(s) 158, 202, 287
NdeI CATATG 2 cut(s) 388, 445
NdeII GATC 2 cut(s) 268, 420
NlaIII CATG 3 cut(s) 190, 287, 420
NlaIV GGNNCC 2 cut(s) 62, 422
PcsI WCGNNNNNNNCGW 1 cut(s) 138
PfeI GAWTC 1 cut(s) 17
PkrI GCNGC 1 cut(s) 151
Psp124BI GAGCTC 1 cut(s) 249
Psp6I CCWGG 2 cut(s) 79, 205
PspGI CCWGG 2 cut(s) 79, 205
PspN4I GGNNCC 2 cut(s) 62, 422
PsuI RGATCY 1 cut(s) 420
RsaI GTAC 5 cut(s) 140, 263, 346, 401, 466
RsaNI GTAC 5 cut(s) 139, 262, 345, 400, 465
SacI GAGCTC 1 cut(s) 249
SaqAI TTAA 1 cut(s) 426
SatI GCNGC 1 cut(s) 150
Sau3AI GATC 2 cut(s) 268, 420
ScrFI CCNGG 2 cut(s) 81, 207
SduI GDGCHC 1 cut(s) 249
SpeI ACTAGT 1 cut(s) 467
Sse9I AATT 3 cut(s) 92, 213, 329
SsiI CCGC 3 cut(s) 5, 116, 384
SspMI CTAG 3 cut(s) 197, 227, 468
SstI GAGCTC 1 cut(s) 249
StyD4I CCNGG 2 cut(s) 79, 205
TaaI ACNGT 1 cut(s) 171
TaiI ACGT 2 cut(s) 135, 354
TasI AATT 3 cut(s) 92, 213, 329
TatI WGTACW 3 cut(s) 344, 399, 464
TfiI GAWTC 1 cut(s) 17
Tru1I TTAA 1 cut(s) 426
Tru9I TTAA 1 cut(s) 426
TscAI CASTG 1 cut(s) 174
TseI GCWGC 1 cut(s) 149
TspDTI ATGAA 1 cut(s) 405
TspGWI ACGGA 1 cut(s) 239
TspRI CASTG 1 cut(s) 174
XspI CTAG 3 cut(s) 197, 227, 468
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.