Rh1BG128200

B3 domain-containing

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1B
OG0008154
Physical Location & Seq
Forward (+)
21634197 .. 21637646
3450 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1BG128200.1

Sequence Viewer

Length: 1848 bp
ATGACTTGTTCTGAGATTGCCTACCCTTGTCAAGAGCAAAGTTCTGATTTGCATTCTATGGGGATTCATGAAGAGGATATGGAAAATGATGGTGTTTGTGACACTTTAGGTACCACAACTCCTCTTTCTCATGCTTCAATGAAAAGAAAATGCAAATGTCTAGACAAACATGTTCTTGAAAGAAGACATGTTTTGCACTCTGGCTCGAATCTTAACAAATGTATCAAAAGAGGTGTTACTAGCAAGGAGAATATGCTATATCAGTCTGACAATGAGACGAGAAGAAAGAAGACTAGATATGTCGATGAAGGAGACACAATCAACTCAAGTAGTGTGACGAAGTCAGTGCCTGCTGGTTTGAAGGAAAATAGAATGCCAAGCTCTCTGGAATTATTAACAAAACAGAATGATGACAAGAAAGTGATCCAGGCTACTAGAATGTTCAAGCCGAAAAATCCTTTTTTCTCAATTATCTTGCAGCCATTTAACTTCACTTATTGCTATTTGCATGTGCCTACTGACTTTGCTGAGAAATATTTGAGTGGGTGTTCAGAATCCATTAAACTTCAGGGTTCTCATGGAAAGCAGTGGGATGTTCAATTTGTGTCTACAAATCCTTCACTTCCAATAATATGCAGGGGTTGCATTCAGTTTTTGACAGACAACAATTTGGAAGAGGGAGATGTCTGTCTTTTTGAGCTGATGAAAAGCAAGGCTGACGTGGTTCTAAAAGTTTCAATACTTCGTGCAGCTGAATATGAAGACACAATAGACTCAAGTAATGTGAACAAATCAAAGCAGGCCAAGCTGAATCAGAATAGAATGTGTGGCTCACAGGATTTCTCCACAGGACAGAACAAAGATGATGGAATGTTTGCTTTTGACATTTTTTCTCACACTTATCCTAAATGGAAACTTAAGTTTCCCAAAGAGACTGAGAAGGTGACCTATGCTACCAGACTGTTCAATCTGAAAAATCCTTTTTTCGCTGTCATCTTGCAGTTCAATAACTACCATCTGTTAATTCCCGTTCCCTTTGCTAGGAGATATTTGAGTAGCTGCGCAGAATTTATCAAACTACAAGATTCCAATGGTGGCCAATGGGATGTCCAATGCAGTTCTACAAATGCTTCAAGTTCAGTCCTTAGGTTCGGCAGGGGATACAATGTATTTTTGACAGACAAGAATTTGGAGCAAGGAGATGTATGTCTCTTTGAGCTGATAAAAAGGAATGATGTTTTGCTAAAAGTTTCAGTACTCCGTGCTTCTGAATATGAAGACATGGTCCATGATAAAGAGAAGAAACTTGATGAGATTAAGAGCAGAAGTTATAGAAATGGACATGTGGAAGAGATACCAGAGCTAGTTGGGAGGCTTAATGAAAGGGGAAAGTCTCCCTCACACAAGTTGTCTGCGGAAGATGAAGGAAACACAGAATCTGGCAATAGTGGAACTTTGTATTATTCTTCTAAGATAGAAAAGATAGTGATGTCCAAAGAAAGCAGGAGGGCGTTCAATGCTGCCAAAAAGTTCAAGACAGAAAATCCTGCCTTCCTTGTATTCTTGCGACATTATCACAAGTCTTTTTTGATAGTGCCTGCTGAATTTCATACTAAGCATCTGGGGTATGAATGCCCAATACTCATTACACTTGAGGCGCCTAATGGAAAGCGTTGTCATGTTCGCTGCATTTATGCTACTGATAGGAAAAAAATATCTGGTGGATGGAGTGCATTCTCCTCAGAAAATAATTTGGAAGAAGGGGATGTTTGTGTATTTGAGCTGACCGCAAGTAAAGATGTTGTGCTGAAGGCTTCAATATTTCGTGCAGCAGAATTTGCGGACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

615

Amino Acids

69.88

Weight (kDa)

8.35

Isoelectric Point (pI)

43.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 154 - 249 2.2e-14 B3 DNA binding domain
B3 PF02362 337 - 422 1.7e-13 B3 DNA binding domain
B3 PF02362 523 - 610 5.6e-15 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1063
Acc65I GGTACC 1 cut(s) 110
AccB1I GGYRCC 2 cut(s) 110, 1657
AccI GTMKAC 1 cut(s) 608
AciI CCGC 3 cut(s) 1415, 1788, 1841
AclWI GGATC 1 cut(s) 418
AcoI YGGCCR 1 cut(s) 1096
AcsI RAATTY 4 cut(s) 1067, 1186, 1604, 1835
AcuI CTGAAG 2 cut(s) 551, 1829
AcyI GRCGYC 1 cut(s) 1658
AfaI GTAC 2 cut(s) 112, 1257
AfiI CCNNNNNNNGG 1 cut(s) 1041
AflII CTTAAG 1 cut(s) 917
AflIII ACRYGT 3 cut(s) 169, 187, 1342
AjiI CACGTC 1 cut(s) 721
AjnI CCWGG 1 cut(s) 426
AluBI AGCT 8 cut(s) 381, 700, 752, 808, 1059, 1219, 1363, 1783
AluI AGCT 8 cut(s) 381, 700, 752, 808, 1059, 1219, 1363, 1783
Alw26I GTCTC 5 cut(s) 269, 306, 926, 1214, 1398
AlwI GGATC 1 cut(s) 418
AlwNI CAGNNNCTG 2 cut(s) 350, 1439
AoxI GGCC 2 cut(s) 801, 1096
ApeKI GCWGC 6 cut(s) 478, 749, 1059, 1520, 1686, 1829
ApoI RAATTY 4 cut(s) 1067, 1186, 1604, 1835
Asp718I GGTACC 1 cut(s) 110
AspLEI GCGC 2 cut(s) 1064, 1660
AspS9I GGNCC 1 cut(s) 1285
AsuHPI GGTGA 1 cut(s) 955
AvaII GGWCC 1 cut(s) 1285
AxyI CCTNAGG 1 cut(s) 1145
BaeI ACNNNNGTAYC 2 cut(s) 205, 238
BalI TGGCCA 1 cut(s) 1098
BanI GGYRCC 2 cut(s) 110, 1657
BbsI GAAGAC 4 cut(s) 190, 296, 768, 1284
BbvI GCAGC 6 cut(s) 490, 761, 1046, 1507, 1673, 1841
BccI CCATC 4 cut(s) 83, 860, 1023, 1719
BcgI CGANNNNNNTGC 2 cut(s) 328, 362
BciT130I CCWGG 1 cut(s) 428
BciVI GTATCC 1 cut(s) 1154
BcoDI GTCTC 5 cut(s) 269, 306, 926, 1214, 1398
BfaI CTAG 7 cut(s) 161, 240, 294, 435, 1041, 1364, 1846
BfoI RGCGCY 1 cut(s) 1661
BfrI CTTAAG 1 cut(s) 917
BfuI GTATCC 1 cut(s) 1154
BisI GCNGC 6 cut(s) 479, 750, 1060, 1521, 1687, 1830
BlsI GCNGC 6 cut(s) 480, 751, 1061, 1522, 1688, 1831
BmcAI AGTACT 1 cut(s) 1257
Bme1390I CCNGG 1 cut(s) 428
Bme18I GGWCC 1 cut(s) 1285
BmgBI CACGTC 1 cut(s) 721
BmgT120I GGNCC 1 cut(s) 1285
BmiI GGNNCC 2 cut(s) 112, 1659
BmrFI CCNGG 1 cut(s) 428
BmsI GCATC 1 cut(s) 1627
BpiI GAAGAC 4 cut(s) 190, 296, 768, 1284
BpuEI CTTGAG 3 cut(s) 310, 760, 1673
BsaHI GRCGYC 1 cut(s) 1658
BsaXI ACNNNNNCTCC 2 cut(s) 103, 133
Bsc4I CCNNNNNNNGG 1 cut(s) 1041
Bse21I CCTNAGG 1 cut(s) 1145
BseBI CCWGG 1 cut(s) 428
BseGI GGATG 4 cut(s) 598, 1111, 1730, 1771
BseLI CCNNNNNNNGG 1 cut(s) 1041
BseMII CTCAG 3 cut(s) 519, 927, 1755
BseRI GAGGAG 2 cut(s) 111, 1729
BseXI GCAGC 6 cut(s) 490, 761, 1046, 1507, 1673, 1841
BsgI GTGCAG 2 cut(s) 768, 1848
BshFI GGCC 2 cut(s) 803, 1098
BshNI GGYRCC 2 cut(s) 110, 1657
BslI CCNNNNNNNGG 1 cut(s) 1041
BsmAI GTCTC 5 cut(s) 269, 306, 926, 1214, 1398
BsmBI CGTCTC 1 cut(s) 269
BsmI GAATGC 5 cut(s) 52, 378, 645, 1637, 1733
BsnI GGCC 2 cut(s) 803, 1098
Bsp143I GATC 1 cut(s) 423
BspACI CCGC 3 cut(s) 1415, 1788, 1841
BspANI GGCC 2 cut(s) 803, 1098
BspCNI CTCAG 4 cut(s) 4, 520, 928, 1754
BspHI TCATGA 1 cut(s) 67
BspLI GGNNCC 2 cut(s) 112, 1659
BspPI GGATC 1 cut(s) 418
BspT107I GGYRCC 2 cut(s) 110, 1657
BspTI CTTAAG 1 cut(s) 917
BssMI GATC 1 cut(s) 423
BssNI GRCGYC 1 cut(s) 1658
Bst2UI CCWGG 1 cut(s) 428
Bst4CI ACNGT 1 cut(s) 963
Bst6I CTCTTC 3 cut(s) 66, 669, 1344
BstACI GRCGYC 1 cut(s) 1658
BstAFI CTTAAG 1 cut(s) 917
BstAPI GCANNNNNTGC 2 cut(s) 642, 1838
BstC8I GCNNGC 3 cut(s) 351, 801, 1599
BstDEI CTNAG 7 cut(s) 12, 528, 936, 1145, 1470, 1614, 1741
BstEII GGTNACC 1 cut(s) 943
BstENI CCTNNNNNAGG 1 cut(s) 1039
BstF5I GGATG 4 cut(s) 598, 1111, 1730, 1771
BstH2I RGCGCY 1 cut(s) 1661
BstHHI GCGC 2 cut(s) 1064, 1660
BstKTI GATC 1 cut(s) 426
BstMAI GTCTC 5 cut(s) 269, 306, 926, 1214, 1398
BstMBI GATC 1 cut(s) 423
BstMWI GCNNNNNNNGC 4 cut(s) 642, 805, 1517, 1838
BstNI CCWGG 1 cut(s) 428
BstNSI RCATGY 4 cut(s) 173, 191, 512, 1346
BstPI GGTNACC 1 cut(s) 943
BstSCI CCNGG 1 cut(s) 426
BstV1I GCAGC 6 cut(s) 490, 761, 1046, 1507, 1673, 1841
BstV2I GAAGAC 4 cut(s) 190, 296, 768, 1284
Bsu36I CCTNAGG 1 cut(s) 1145
BsuI GTATCC 1 cut(s) 1154
BsuRI GGCC 2 cut(s) 803, 1098
BtrI CACGTC 1 cut(s) 721
BtsCI GGATG 4 cut(s) 598, 1111, 1730, 1771
BtsI GCAGTG 1 cut(s) 593
BtsIMutI CAGTG 2 cut(s) 351, 593
Cac8I GCNNGC 3 cut(s) 351, 801, 1599
CaiI CAGNNNCTG 2 cut(s) 350, 1439
CciI TCATGA 1 cut(s) 67
CfoI GCGC 2 cut(s) 1064, 1660
Cfr13I GGNCC 1 cut(s) 1285
Csp6I GTAC 2 cut(s) 111, 1256
CviQI GTAC 2 cut(s) 111, 1256
DdeI CTNAG 7 cut(s) 12, 528, 936, 1145, 1470, 1614, 1741
DinI GGCGCC 1 cut(s) 1659
DpnI GATC 1 cut(s) 425
DpnII GATC 1 cut(s) 423
EaeI YGGCCR 1 cut(s) 1096
Eam1104I CTCTTC 3 cut(s) 66, 669, 1344
EarI CTCTTC 3 cut(s) 66, 669, 1344
Eco47I GGWCC 1 cut(s) 1285
Eco57I CTGAAG 2 cut(s) 551, 1829
Eco81I CCTNAGG 1 cut(s) 1145
Eco91I GGTNACC 1 cut(s) 943
EcoNI CCTNNNNNAGG 1 cut(s) 1039
EcoO65I GGTNACC 1 cut(s) 943
EcoRII CCWGG 1 cut(s) 426
EgeI GGCGCC 1 cut(s) 1659
EheI GGCGCC 1 cut(s) 1659
Esp3I CGTCTC 1 cut(s) 269
FblI GTMKAC 1 cut(s) 608
Fnu4HI GCNGC 6 cut(s) 479, 750, 1060, 1521, 1687, 1830
FokI GGATG 4 cut(s) 605, 1118, 1737, 1778
Fsp4HI GCNGC 6 cut(s) 479, 750, 1060, 1521, 1687, 1830
FspBI CTAG 7 cut(s) 161, 240, 294, 435, 1041, 1364, 1846
FspI TGCGCA 1 cut(s) 1063
GlaI GCGC 2 cut(s) 1063, 1659
GluI GCNGC 6 cut(s) 479, 750, 1060, 1521, 1687, 1830
HaeII RGCGCY 1 cut(s) 1661
HaeIII GGCC 2 cut(s) 803, 1098
HhaI GCGC 2 cut(s) 1064, 1660
Hin1I GRCGYC 1 cut(s) 1658
Hin6I GCGC 2 cut(s) 1062, 1658
HinP1I GCGC 2 cut(s) 1062, 1658
HinfI GANTC 7 cut(s) 64, 208, 554, 773, 811, 1085, 1436
HphI GGTGA 1 cut(s) 955
Hpy166II GTNNAC 2 cut(s) 609, 787
Hpy188I TCNGA 8 cut(s) 13, 46, 268, 553, 816, 972, 1270, 1744
Hpy188III TCNNGA 6 cut(s) 32, 68, 161, 176, 386, 1534
Hpy8I GTNNAC 2 cut(s) 609, 787
HpyAV CCTTC 8 cut(s) 302, 355, 627, 934, 1418, 1561, 1754, 1804
HpyCH4III ACNGT 1 cut(s) 963
HpyCH4IV ACGT 1 cut(s) 720
HpyF10VI GCNNNNNNNGC 4 cut(s) 642, 805, 1517, 1838
HpyF3I CTNAG 7 cut(s) 12, 528, 936, 1145, 1470, 1614, 1741
HpySE526I ACGT 1 cut(s) 720
Hsp92I GRCGYC 1 cut(s) 1658
HspAI GCGC 2 cut(s) 1062, 1658
KasI GGCGCC 1 cut(s) 1657
KpnI GGTACC 1 cut(s) 114
Kzo9I GATC 1 cut(s) 423
LmnI GCTCC 1 cut(s) 1192
Lsp1109I GCAGC 6 cut(s) 490, 761, 1046, 1507, 1673, 1841
LweI GCATC 1 cut(s) 1627
MaeI CTAG 7 cut(s) 161, 240, 294, 435, 1041, 1364, 1846
MaeII ACGT 1 cut(s) 720
MaeIII GTNAC 4 cut(s) 98, 235, 334, 943
MalI GATC 1 cut(s) 425
MboI GATC 1 cut(s) 423
MlsI TGGCCA 1 cut(s) 1098
MluNI TGGCCA 1 cut(s) 1098
Mly113I GGCGCC 1 cut(s) 1658
MlyI GAGTC 1 cut(s) 767
MnlI CCTC 9 cut(s) 67, 132, 224, 670, 1365, 1408, 1500, 1648, 1750
Mox20I TGGCCA 1 cut(s) 1098
MscI TGGCCA 1 cut(s) 1098
MseI TTAA 8 cut(s) 213, 395, 486, 561, 918, 1022, 1317, 1377
Msp20I TGGCCA 1 cut(s) 1098
MspA1I CMGCKG 1 cut(s) 752
MspCI CTTAAG 1 cut(s) 917
MspR9I CCNGG 1 cut(s) 428
Mva1269I GAATGC 5 cut(s) 52, 378, 645, 1637, 1733
MvaI CCWGG 1 cut(s) 428
MwoI GCNNNNNNNGC 4 cut(s) 642, 805, 1517, 1838
NarI GGCGCC 1 cut(s) 1658
NdeII GATC 1 cut(s) 423
NlaIV GGNNCC 2 cut(s) 112, 1659
NmuCI GTSAC 3 cut(s) 98, 334, 943
NsbI TGCGCA 1 cut(s) 1063
NspI RCATGY 4 cut(s) 173, 191, 512, 1346
PagI TCATGA 1 cut(s) 67
PciI ACATGT 3 cut(s) 169, 187, 1342
PctI GAATGC 5 cut(s) 52, 378, 645, 1637, 1733
PfeI GAWTC 6 cut(s) 64, 208, 554, 811, 1085, 1436
PflFI GACNNNGTC 2 cut(s) 340, 1283
PkrI GCNGC 6 cut(s) 480, 751, 1061, 1522, 1688, 1831
PleI GAGTC 1 cut(s) 767
PluTI GGCGCC 1 cut(s) 1661
PpsI GAGTC 1 cut(s) 767
PscI ACATGT 3 cut(s) 169, 187, 1342
Psp6I CCWGG 1 cut(s) 426
PspEI GGTNACC 1 cut(s) 943
PspGI CCWGG 1 cut(s) 426
PspN4I GGNNCC 2 cut(s) 112, 1659
PspPI GGNCC 1 cut(s) 1285
PstNI CAGNNNCTG 2 cut(s) 350, 1439
PsyI GACNNNGTC 2 cut(s) 340, 1283
PvuII CAGCTG 1 cut(s) 752
RsaI GTAC 2 cut(s) 112, 1257
RsaNI GTAC 2 cut(s) 111, 1256
SaqAI TTAA 8 cut(s) 213, 395, 486, 561, 918, 1022, 1317, 1377
SatI GCNGC 6 cut(s) 479, 750, 1060, 1521, 1687, 1830
Sau3AI GATC 1 cut(s) 423
Sau96I GGNCC 1 cut(s) 1285
ScaI AGTACT 1 cut(s) 1257
SchI GAGTC 1 cut(s) 767
ScrFI CCNGG 1 cut(s) 428
SfaNI GCATC 1 cut(s) 1627
SfoI GGCGCC 1 cut(s) 1659
SinI GGWCC 1 cut(s) 1285
SmlI CTYRAG 4 cut(s) 325, 775, 917, 1652
SmoI CTYRAG 4 cut(s) 325, 775, 917, 1652
SsiI CCGC 3 cut(s) 1415, 1788, 1841
SspDI GGCGCC 1 cut(s) 1657
SspI AATATT 2 cut(s) 536, 1821
SspMI CTAG 7 cut(s) 161, 240, 294, 435, 1041, 1364, 1846
StyD4I CCNGG 1 cut(s) 426
TaaI ACNGT 1 cut(s) 963
TaiI ACGT 1 cut(s) 723
TaqI TCGA 2 cut(s) 206, 303
TatI WGTACW 1 cut(s) 1255
TfiI GAWTC 6 cut(s) 64, 208, 554, 811, 1085, 1436
Tru1I TTAA 8 cut(s) 213, 395, 486, 561, 918, 1022, 1317, 1377
Tru9I TTAA 8 cut(s) 213, 395, 486, 561, 918, 1022, 1317, 1377
TscAI CASTG 2 cut(s) 351, 593
TseFI GTSAC 3 cut(s) 98, 334, 943
TseI GCWGC 6 cut(s) 478, 749, 1059, 1520, 1686, 1829
Tsp45I GTSAC 3 cut(s) 98, 334, 943
TspGWI ACGGA 1 cut(s) 1250
TspRI CASTG 2 cut(s) 351, 593
Tth111I GACNNNGTC 2 cut(s) 340, 1283
Vha464I CTTAAG 1 cut(s) 917
VpaK11BI GGWCC 1 cut(s) 1285
XagI CCTNNNNNAGG 1 cut(s) 1039
XapI RAATTY 4 cut(s) 1067, 1186, 1604, 1835
XbaI TCTAGA 1 cut(s) 160
XceI RCATGY 4 cut(s) 173, 191, 512, 1346
XmiI GTMKAC 1 cut(s) 608
XspI CTAG 7 cut(s) 161, 240, 294, 435, 1041, 1364, 1846
ZrmI AGTACT 1 cut(s) 1257
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.