Rh1DG004100

60S ribosomal Protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0020429
Physical Location & Seq
Forward (+)
601554 .. 610289
8736 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG004100.1

Sequence Viewer

Length: 192 bp
ATGGTTCTCAAGACTGAGCTTTGCCGCTTCAGTGGCGCCGAGATCTACCCGGGTAGAGGCATCAGATTCATCCGCTCCGATTCTCAGATGATAATCGAAAAGATAAGATTGAGGGGTCCAAAGGGCAATCTCCGTGAATCTGATATTGTCGCTGCTCAAGTCATTGACGGATCACAAATCCGATTAAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L24e

Protein Analysis

63

Amino Acids

7.13

Weight (kDa)

10.16

Isoelectric Point (pI)

35.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L24e PF01246 3 - 31 8.8e-10 Ribosomal protein L24e
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0020429)

Species Orthologous Gene IDs
pyrus_communis pycom02g03100 pycom15g15970
rosa_multiflora Rmu_sc0004339.1_g000025 Rmu_sc0024200.1_g000001
rosa_samantha Rh1DG004100 Rh4AG353800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 35
AccBSI CCGCTC 1 cut(s) 75
AciI CCGC 2 cut(s) 25, 73
AclWI GGATC 1 cut(s) 178
AcuI CTGAAG 1 cut(s) 13
AcyI GRCGYC 1 cut(s) 36
AfiI CCNNNNNNNGG 1 cut(s) 56
AluBI AGCT 1 cut(s) 19
AluI AGCT 1 cut(s) 19
AlwI GGATC 1 cut(s) 178
Ama87I CYCGRG 1 cut(s) 49
ApeKI GCWGC 1 cut(s) 152
AspLEI GCGC 1 cut(s) 38
AspS9I GGNCC 1 cut(s) 116
AsuC2I CCSGG 2 cut(s) 50, 51
AvaI CYCGRG 1 cut(s) 49
AvaII GGWCC 1 cut(s) 116
BanI GGYRCC 1 cut(s) 35
BbvI GCAGC 1 cut(s) 139
BcnI CCSGG 2 cut(s) 50, 51
BfoI RGCGCY 1 cut(s) 39
BglII AGATCT 1 cut(s) 42
BisI GCNGC 2 cut(s) 25, 153
BlsI GCNGC 2 cut(s) 26, 154
Bme1390I CCNGG 2 cut(s) 50, 51
Bme18I GGWCC 1 cut(s) 116
BmeT110I CYCGRG 1 cut(s) 49
BmgT120I GGNCC 1 cut(s) 116
BmiI GGNNCC 2 cut(s) 37, 117
BmrFI CCNGG 2 cut(s) 50, 51
BmsI GCATC 1 cut(s) 69
BpuEI CTTGAG 1 cut(s) 141
BpuMI CCSGG 2 cut(s) 50, 51
BsaBI GATNNNNATC 1 cut(s) 92
BsaHI GRCGYC 1 cut(s) 36
BsaJI CCNNGG 1 cut(s) 49
Bsc4I CCNNNNNNNGG 1 cut(s) 56
Bse8I GATNNNNATC 1 cut(s) 92
BseDI CCNNGG 1 cut(s) 49
BseGI GGATG 1 cut(s) 69
BseJI GATNNNNATC 1 cut(s) 92
BseLI CCNNNNNNNGG 1 cut(s) 56
BseMII CTCAG 2 cut(s) 6, 98
BseXI GCAGC 1 cut(s) 139
BshNI GGYRCC 1 cut(s) 35
BsiHKCI CYCGRG 1 cut(s) 49
BsiSI CCGG 1 cut(s) 50
BslI CCNNNNNNNGG 1 cut(s) 56
BsoBI CYCGRG 1 cut(s) 49
Bsp143I GATC 2 cut(s) 42, 170
BspACI CCGC 2 cut(s) 25, 73
BspCNI CTCAG 2 cut(s) 7, 97
BspLI GGNNCC 2 cut(s) 37, 117
BspPI GGATC 1 cut(s) 178
BspT107I GGYRCC 1 cut(s) 35
BsrBI CCGCTC 1 cut(s) 75
BssECI CCNNGG 1 cut(s) 49
BssMI GATC 2 cut(s) 42, 170
BssNI GRCGYC 1 cut(s) 36
BstACI GRCGYC 1 cut(s) 36
BstDEI CTNAG 2 cut(s) 15, 84
BstF5I GGATG 1 cut(s) 69
BstH2I RGCGCY 1 cut(s) 39
BstHHI GCGC 1 cut(s) 38
BstKTI GATC 2 cut(s) 45, 173
BstMBI GATC 2 cut(s) 42, 170
BstMWI GCNNNNNNNGC 1 cut(s) 33
BstSCI CCNGG 2 cut(s) 48, 49
BstV1I GCAGC 1 cut(s) 139
BstX2I RGATCY 1 cut(s) 42
BstYI RGATCY 1 cut(s) 42
BtsCI GGATG 1 cut(s) 69
BtsIMutI CAGTG 1 cut(s) 37
CfoI GCGC 1 cut(s) 38
Cfr13I GGNCC 1 cut(s) 116
Cfr9I CCCGGG 1 cut(s) 49
CviJI RGCY 1 cut(s) 19
CviKI_1 RGCY 1 cut(s) 19
DdeI CTNAG 2 cut(s) 15, 84
DinI GGCGCC 1 cut(s) 37
DpnI GATC 2 cut(s) 44, 172
DpnII GATC 2 cut(s) 42, 170
Eco47I GGWCC 1 cut(s) 116
Eco57I CTGAAG 1 cut(s) 13
Eco88I CYCGRG 1 cut(s) 49
EgeI GGCGCC 1 cut(s) 37
EheI GGCGCC 1 cut(s) 37
Fnu4HI GCNGC 2 cut(s) 25, 153
FokI GGATG 1 cut(s) 56
Fsp4HI GCNGC 2 cut(s) 25, 153
GlaI GCGC 1 cut(s) 37
GluI GCNGC 2 cut(s) 25, 153
HaeII RGCGCY 1 cut(s) 39
HapII CCGG 1 cut(s) 50
HhaI GCGC 1 cut(s) 38
Hin1I GRCGYC 1 cut(s) 36
Hin6I GCGC 1 cut(s) 36
HinP1I GCGC 1 cut(s) 36
HinfI GANTC 3 cut(s) 66, 80, 137
HpaII CCGG 1 cut(s) 50
Hpy188I TCNGA 5 cut(s) 65, 79, 87, 142, 182
Hpy188III TCNNGA 1 cut(s) 10
HpyF10VI GCNNNNNNNGC 1 cut(s) 33
HpyF3I CTNAG 2 cut(s) 15, 84
Hsp92I GRCGYC 1 cut(s) 36
HspAI GCGC 1 cut(s) 36
KasI GGCGCC 1 cut(s) 35
Kzo9I GATC 2 cut(s) 42, 170
LmnI GCTCC 1 cut(s) 80
LpnPI CCDG 1 cut(s) 63
Lsp1109I GCAGC 1 cut(s) 139
LweI GCATC 1 cut(s) 69
MalI GATC 2 cut(s) 44, 172
MbiI CCGCTC 1 cut(s) 75
MboI GATC 2 cut(s) 42, 170
MflI RGATCY 1 cut(s) 42
Mly113I GGCGCC 1 cut(s) 36
MnlI CCTC 2 cut(s) 50, 105
MseI TTAA 1 cut(s) 185
MspI CCGG 1 cut(s) 50
MspR9I CCNGG 2 cut(s) 50, 51
MwoI GCNNNNNNNGC 1 cut(s) 33
NarI GGCGCC 1 cut(s) 36
NciI CCSGG 2 cut(s) 50, 51
NdeII GATC 2 cut(s) 42, 170
NlaIV GGNNCC 2 cut(s) 37, 117
NmeAIII GCCGAG 1 cut(s) 64
PfeI GAWTC 3 cut(s) 66, 80, 137
PkrI GCNGC 2 cut(s) 26, 154
PluTI GGCGCC 1 cut(s) 39
PspN4I GGNNCC 2 cut(s) 37, 117
PspPI GGNCC 1 cut(s) 116
PsuI RGATCY 1 cut(s) 42
SaqAI TTAA 1 cut(s) 185
SatI GCNGC 2 cut(s) 25, 153
Sau3AI GATC 2 cut(s) 42, 170
Sau96I GGNCC 1 cut(s) 116
ScrFI CCNGG 2 cut(s) 50, 51
SetI ASST 1 cut(s) 21
SfaNI GCATC 1 cut(s) 69
SfoI GGCGCC 1 cut(s) 37
SgeI CNNG 7 cut(s) 22, 52, 61, 62, 63, 146, 170
SinI GGWCC 1 cut(s) 116
SmaI CCCGGG 1 cut(s) 51
SmlI CTYRAG 2 cut(s) 8, 156
SmoI CTYRAG 2 cut(s) 8, 156
SsiI CCGC 2 cut(s) 25, 73
SspDI GGCGCC 1 cut(s) 35
StyD4I CCNGG 2 cut(s) 48, 49
TaqI TCGA 1 cut(s) 96
TauI GCSGC 1 cut(s) 27
TfiI GAWTC 3 cut(s) 66, 80, 137
Tru1I TTAA 1 cut(s) 185
Tru9I TTAA 1 cut(s) 185
TscAI CASTG 1 cut(s) 37
TseI GCWGC 1 cut(s) 152
TspDTI ATGAA 1 cut(s) 58
TspGWI ACGGA 2 cut(s) 122, 183
TspMI CCCGGG 1 cut(s) 49
TspRI CASTG 1 cut(s) 37
VpaK11BI GGWCC 1 cut(s) 116
XmaI CCCGGG 1 cut(s) 49
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.