Rh2AG121800

Belongs to the MIP aquaporin (TC 1.A.8) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0019775
Physical Location & Seq
Reverse (-)
11038271 .. 11038741
471 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG121800.1

Sequence Viewer

Length: 321 bp
ATGACTTCCAGAAACAGAAAAGTATATGGATGTAAGTACCTAACATATTGCTTGGTTCAGGTATTGGTTCCATTACCAATAGGGTTTGCTGTGTTTATGGTTCACCTGGCCACCATACCTATTACTGGGACTGGGATCAACCCTGCTAGGAGTTCTGCTGCTGCTGTAATCTACAACAATGACAAAGTCTGGGACGACCAGTGGATATTCTGGGTCGGACCATTCATTGGAGCTCTGATAGCAGCAGCTTATAACCAGTACGTACTGAGGGCATCAGCTAGTAAAGCCTTCAGATCGTTCCACAACAATACCACCAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MIP

Protein Analysis

106

Amino Acids

11.79

Weight (kDa)

9.7

Isoelectric Point (pI)

21.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MIP PF00230 22 - 83 2.4e-22 Major intrinsic protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019775)

Species Orthologous Gene IDs
malus_domestica MD00G1173200.v1.1 MD07G1136400.v1.1
pyrus_communis pycom07g13370
rosa_chinensis RchiOBHm_Chr2g0098661
rosa_multiflora Rmu_co8012854.1_g000001
rosa_samantha Rh2AG121800 Rh2CG126400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 252
AccB7I CCANNNNNTGG 1 cut(s) 227
AclWI GGATC 1 cut(s) 143
AcoI YGGCCR 1 cut(s) 108
AcuI CTGAAG 1 cut(s) 274
AfaI GTAC 3 cut(s) 38, 260, 264
AfiI CCNNNNNNNGG 2 cut(s) 125, 227
AjnI CCWGG 1 cut(s) 105
AluBI AGCT 3 cut(s) 233, 248, 278
AluI AGCT 3 cut(s) 233, 248, 278
Alw21I GWGCWC 1 cut(s) 235
AlwI GGATC 1 cut(s) 143
AoxI GGCC 1 cut(s) 108
ApeKI GCWGC 4 cut(s) 158, 161, 242, 245
AspS9I GGNCC 1 cut(s) 218
AsuHPI GGTGA 1 cut(s) 95
AvaII GGWCC 1 cut(s) 218
BalI TGGCCA 1 cut(s) 110
BanII GRGCYC 1 cut(s) 235
Bbv12I GWGCWC 1 cut(s) 235
BbvI GCAGC 4 cut(s) 145, 148, 254, 257
BciT130I CCWGG 1 cut(s) 107
BfaI CTAG 2 cut(s) 147, 279
BisI GCNGC 4 cut(s) 159, 162, 243, 246
BlsI GCNGC 4 cut(s) 160, 163, 244, 247
Bme1390I CCNGG 1 cut(s) 107
Bme18I GGWCC 1 cut(s) 218
BmgT120I GGNCC 1 cut(s) 218
BmiI GGNNCC 1 cut(s) 69
BmrFI CCNGG 1 cut(s) 107
BmrI ACTGGG 2 cut(s) 135, 141
BmsI GCATC 1 cut(s) 281
BmuI ACTGGG 2 cut(s) 135, 141
BsaAI YACGTR 1 cut(s) 262
Bsc4I CCNNNNNNNGG 2 cut(s) 125, 227
Bse1I ACTGG 4 cut(s) 130, 136, 199, 256
BseBI CCWGG 1 cut(s) 107
BseGI GGATG 1 cut(s) 35
BseLI CCNNNNNNNGG 2 cut(s) 125, 227
BseMII CTCAG 1 cut(s) 257
BseNI ACTGG 4 cut(s) 130, 136, 199, 256
BseXI GCAGC 4 cut(s) 145, 148, 254, 257
BshFI GGCC 1 cut(s) 110
BsiHKAI GWGCWC 1 cut(s) 235
BslFI GGGAC 2 cut(s) 142, 206
BslI CCNNNNNNNGG 2 cut(s) 125, 227
BsmFI GGGAC 2 cut(s) 142, 206
BsnI GGCC 1 cut(s) 110
Bsp1286I GDGCHC 1 cut(s) 235
Bsp143I GATC 2 cut(s) 135, 293
BspANI GGCC 1 cut(s) 110
BspCNI CTCAG 1 cut(s) 258
BspLI GGNNCC 1 cut(s) 69
BspPI GGATC 1 cut(s) 143
BsrI ACTGG 4 cut(s) 130, 136, 199, 256
BssMI GATC 2 cut(s) 135, 293
Bst2UI CCWGG 1 cut(s) 107
BstBAI YACGTR 1 cut(s) 262
BstDEI CTNAG 1 cut(s) 266
BstF5I GGATG 1 cut(s) 35
BstKTI GATC 2 cut(s) 138, 296
BstMBI GATC 2 cut(s) 135, 293
BstMWI GCNNNNNNNGC 2 cut(s) 239, 284
BstNI CCWGG 1 cut(s) 107
BstSCI CCNGG 1 cut(s) 105
BstSNI TACGTA 1 cut(s) 262
BstV1I GCAGC 4 cut(s) 145, 148, 254, 257
BsuRI GGCC 1 cut(s) 110
BtsCI GGATG 1 cut(s) 35
BtsIMutI CAGTG 1 cut(s) 206
Cfr13I GGNCC 1 cut(s) 218
Csp6I GTAC 3 cut(s) 37, 259, 263
CviJI RGCY 5 cut(s) 110, 233, 248, 278, 287
CviKI_1 RGCY 5 cut(s) 110, 233, 248, 278, 287
CviQI GTAC 3 cut(s) 37, 259, 263
DdeI CTNAG 1 cut(s) 266
DpnI GATC 2 cut(s) 137, 295
DpnII GATC 2 cut(s) 135, 293
EaeI YGGCCR 1 cut(s) 108
Ecl136II GAGCTC 1 cut(s) 233
Eco105I TACGTA 1 cut(s) 262
Eco24I GRGCYC 1 cut(s) 235
Eco47I GGWCC 1 cut(s) 218
Eco53kI GAGCTC 1 cut(s) 233
Eco57I CTGAAG 1 cut(s) 274
EcoICRI GAGCTC 1 cut(s) 233
EcoRII CCWGG 1 cut(s) 105
EcoT38I GRGCYC 1 cut(s) 235
FaiI YATR 6 cut(s) 25, 27, 46, 98, 116, 252
FaqI GGGAC 2 cut(s) 142, 206
Fnu4HI GCNGC 4 cut(s) 159, 162, 243, 246
FokI GGATG 1 cut(s) 42
FriOI GRGCYC 1 cut(s) 235
Fsp4HI GCNGC 4 cut(s) 159, 162, 243, 246
FspBI CTAG 2 cut(s) 147, 279
GluI GCNGC 4 cut(s) 159, 162, 243, 246
HaeIII GGCC 1 cut(s) 110
HphI GGTGA 1 cut(s) 95
Hpy166II GTNNAC 1 cut(s) 103
Hpy188I TCNGA 3 cut(s) 218, 237, 293
Hpy188III TCNNGA 1 cut(s) 9
Hpy8I GTNNAC 1 cut(s) 103
HpyAV CCTTC 1 cut(s) 298
HpyCH4IV ACGT 1 cut(s) 261
HpyF10VI GCNNNNNNNGC 2 cut(s) 239, 284
HpyF3I CTNAG 1 cut(s) 266
HpySE526I ACGT 1 cut(s) 261
Kzo9I GATC 2 cut(s) 135, 293
LmnI GCTCC 1 cut(s) 230
Lsp1109I GCAGC 4 cut(s) 145, 148, 254, 257
LweI GCATC 1 cut(s) 281
MaeI CTAG 2 cut(s) 147, 279
MaeII ACGT 1 cut(s) 261
MalI GATC 2 cut(s) 137, 295
MboI GATC 2 cut(s) 135, 293
MhlI GDGCHC 1 cut(s) 235
MlsI TGGCCA 1 cut(s) 110
MluNI TGGCCA 1 cut(s) 110
MmeI TCCRAC 1 cut(s) 196
MnlI CCTC 1 cut(s) 261
Mox20I TGGCCA 1 cut(s) 110
MscI TGGCCA 1 cut(s) 110
Msp20I TGGCCA 1 cut(s) 110
MspR9I CCNGG 1 cut(s) 107
MvaI CCWGG 1 cut(s) 107
MwoI GCNNNNNNNGC 2 cut(s) 239, 284
NdeII GATC 2 cut(s) 135, 293
NlaIV GGNNCC 1 cut(s) 69
PflFI GACNNNGTC 1 cut(s) 185
PflMI CCANNNNNTGG 1 cut(s) 227
PkrI GCNGC 4 cut(s) 160, 163, 244, 247
Ppu21I YACGTR 1 cut(s) 262
PsiI TTATAA 1 cut(s) 252
Psp124BI GAGCTC 1 cut(s) 235
Psp6I CCWGG 1 cut(s) 105
PspGI CCWGG 1 cut(s) 105
PspN4I GGNNCC 1 cut(s) 69
PspPI GGNCC 1 cut(s) 218
PsyI GACNNNGTC 1 cut(s) 185
RsaI GTAC 3 cut(s) 38, 260, 264
RsaNI GTAC 3 cut(s) 37, 259, 263
SacI GAGCTC 1 cut(s) 235
SatI GCNGC 4 cut(s) 159, 162, 243, 246
Sau3AI GATC 2 cut(s) 135, 293
Sau96I GGNCC 1 cut(s) 218
ScrFI CCNGG 1 cut(s) 107
SduI GDGCHC 1 cut(s) 235
SetI ASST 8 cut(s) 42, 63, 108, 121, 235, 250, 264, 280
SfaNI GCATC 1 cut(s) 281
SinI GGWCC 1 cut(s) 218
SnaBI TACGTA 1 cut(s) 262
SspMI CTAG 2 cut(s) 147, 279
SstI GAGCTC 1 cut(s) 235
StyD4I CCNGG 1 cut(s) 105
TaiI ACGT 1 cut(s) 264
TscAI CASTG 1 cut(s) 206
TseI GCWGC 4 cut(s) 158, 161, 242, 245
TspDTI ATGAA 1 cut(s) 214
TspRI CASTG 1 cut(s) 206
Tth111I GACNNNGTC 1 cut(s) 185
Van91I CCANNNNNTGG 1 cut(s) 227
VpaK11BI GGWCC 1 cut(s) 218
XspI CTAG 2 cut(s) 147, 279
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.