Rh2AG233600

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0020724
Physical Location & Seq
Forward (+)
25191364 .. 25191666
303 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG233600.1

Sequence Viewer

Length: 303 bp
ATGGTGCACAACTGCTATCTCTGCCACTTCCATAGGTTGGGGTGCAAAGGCTGGCGCCGCCATCAATCGGAGTATGGGCGCTTTGATCCGGGTCCAGAGGTCGCATCCTCTCATCTCTTGACTTCGAGACTGTATATTCAGGTCCTTCCCGACTGCGTACAGCCCAGATTTGAGACGTCCTCGCCTCGGCCTGATCCGCCATGGCCATTGCTCCACCCAATCACCATTGAATCTGATAGGACCGGAGCACCAATCCATCTCTCTCACATTACCTATTTCCTTCCGGCAATAGATGATGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

100

Amino Acids

11.56

Weight (kDa)

6.42

Isoelectric Point (pI)

58.73

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0020724)

Species Orthologous Gene IDs
rosa_multiflora Rmu_co8212208.1_g000001 Rmu_sc0000570.1_g000023
rosa_roxburghii Rroxscaffold_2G00099200
rosa_samantha Rh2AG233600 Rh2AG439200 Rh4DG071100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 179
AccB1I GGYRCC 1 cut(s) 54
AccB7I CCANNNNNTGG 1 cut(s) 37
AciI CCGC 2 cut(s) 58, 197
AclWI GGATC 2 cut(s) 80, 188
AcoI YGGCCR 1 cut(s) 203
AcyI GRCGYC 2 cut(s) 55, 176
AfaI GTAC 1 cut(s) 159
AfiI CCNNNNNNNGG 3 cut(s) 37, 67, 186
AgsI TTSAA 1 cut(s) 230
Alw21I GWGCWC 2 cut(s) 9, 250
Alw26I GTCTC 2 cut(s) 121, 167
Alw44I GTGCAC 1 cut(s) 5
AlwI GGATC 2 cut(s) 80, 188
AoxI GGCC 2 cut(s) 188, 203
ApaLI GTGCAC 1 cut(s) 5
AspLEI GCGC 2 cut(s) 57, 81
AspS9I GGNCC 3 cut(s) 92, 142, 240
AsuC2I CCSGG 1 cut(s) 90
AsuHPI GGTGA 1 cut(s) 214
AvaII GGWCC 3 cut(s) 92, 142, 240
BaeGI GKGCMC 1 cut(s) 9
BalI TGGCCA 1 cut(s) 205
BanI GGYRCC 1 cut(s) 54
Bbv12I GWGCWC 2 cut(s) 9, 250
BccI CCATC 2 cut(s) 69, 264
BcnI CCSGG 1 cut(s) 90
BcoDI GTCTC 2 cut(s) 121, 167
BfoI RGCGCY 2 cut(s) 58, 82
BisI GCNGC 1 cut(s) 58
BlsI GCNGC 1 cut(s) 59
Bme1390I CCNGG 1 cut(s) 90
Bme18I GGWCC 3 cut(s) 92, 142, 240
BmgT120I GGNCC 3 cut(s) 92, 142, 240
BmiI GGNNCC 2 cut(s) 56, 93
BmrFI CCNGG 1 cut(s) 90
BmsI GCATC 1 cut(s) 113
BplI GAGNNNNNCTC 2 cut(s) 164, 196
BpuMI CCSGG 1 cut(s) 90
BsaHI GRCGYC 2 cut(s) 55, 176
BsaJI CCNNGG 2 cut(s) 185, 200
BsaWI WCCGGW 1 cut(s) 242
Bsc4I CCNNNNNNNGG 3 cut(s) 37, 67, 186
Bse3DI GCAATG 1 cut(s) 206
BseDI CCNNGG 2 cut(s) 185, 200
BseGI GGATG 1 cut(s) 104
BseLI CCNNNNNNNGG 3 cut(s) 37, 67, 186
BseMI GCAATG 1 cut(s) 206
BseSI GKGCMC 1 cut(s) 9
BshFI GGCC 2 cut(s) 190, 205
BshNI GGYRCC 1 cut(s) 54
BsiHKAI GWGCWC 2 cut(s) 9, 250
BsiSI CCGG 3 cut(s) 89, 243, 284
BslI CCNNNNNNNGG 3 cut(s) 37, 67, 186
BsmAI GTCTC 2 cut(s) 121, 167
BsmBI CGTCTC 1 cut(s) 167
BsnI GGCC 2 cut(s) 190, 205
Bsp1286I GDGCHC 2 cut(s) 9, 250
Bsp143I GATC 2 cut(s) 85, 193
Bsp19I CCATGG 1 cut(s) 200
BspACI CCGC 2 cut(s) 58, 197
BspANI GGCC 2 cut(s) 190, 205
BspLI GGNNCC 2 cut(s) 56, 93
BspPI GGATC 2 cut(s) 80, 188
BspT107I GGYRCC 1 cut(s) 54
BsrDI GCAATG 1 cut(s) 206
BssECI CCNNGG 2 cut(s) 185, 200
BssMI GATC 2 cut(s) 85, 193
BssNI GRCGYC 2 cut(s) 55, 176
BssT1I CCWWGG 1 cut(s) 200
Bst4CI ACNGT 1 cut(s) 132
BstACI GRCGYC 2 cut(s) 55, 176
BstC8I GCNNGC 1 cut(s) 53
BstDSI CCRYGG 1 cut(s) 200
BstF5I GGATG 1 cut(s) 104
BstH2I RGCGCY 2 cut(s) 58, 82
BstHHI GCGC 2 cut(s) 57, 81
BstKTI GATC 2 cut(s) 88, 196
BstMAI GTCTC 2 cut(s) 121, 167
BstMBI GATC 2 cut(s) 85, 193
BstMWI GCNNNNNNNGC 3 cut(s) 21, 57, 196
BstSCI CCNGG 1 cut(s) 88
BstSLI GKGCMC 1 cut(s) 9
BsuRI GGCC 2 cut(s) 190, 205
BtgI CCRYGG 1 cut(s) 200
BtsCI GGATG 1 cut(s) 104
Cac8I GCNNGC 1 cut(s) 53
CfoI GCGC 2 cut(s) 57, 81
Cfr13I GGNCC 3 cut(s) 92, 142, 240
Csp6I GTAC 1 cut(s) 158
CviAII CATG 1 cut(s) 201
CviJI RGCY 4 cut(s) 51, 163, 190, 205
CviKI_1 RGCY 4 cut(s) 51, 163, 190, 205
CviQI GTAC 1 cut(s) 158
DinI GGCGCC 1 cut(s) 56
DpnI GATC 2 cut(s) 87, 195
DpnII GATC 2 cut(s) 85, 193
EaeI YGGCCR 1 cut(s) 203
EciI GGCGGA 1 cut(s) 186
Eco130I CCWWGG 1 cut(s) 200
Eco47I GGWCC 3 cut(s) 92, 142, 240
EcoO109I RGGNCCY 1 cut(s) 142
EcoT14I CCWWGG 1 cut(s) 200
EgeI GGCGCC 1 cut(s) 56
EheI GGCGCC 1 cut(s) 56
ErhI CCWWGG 1 cut(s) 200
Esp3I CGTCTC 1 cut(s) 167
FaeI CATG 1 cut(s) 204
FaiI YATR 4 cut(s) 33, 75, 135, 202
FatI CATG 1 cut(s) 200
Fnu4HI GCNGC 1 cut(s) 58
FokI GGATG 1 cut(s) 91
Fsp4HI GCNGC 1 cut(s) 58
GlaI GCGC 2 cut(s) 56, 80
GluI GCNGC 1 cut(s) 58
HaeII RGCGCY 2 cut(s) 58, 82
HaeIII GGCC 2 cut(s) 190, 205
HapII CCGG 3 cut(s) 89, 243, 284
HhaI GCGC 2 cut(s) 57, 81
Hin1I GRCGYC 2 cut(s) 55, 176
Hin1II CATG 1 cut(s) 204
Hin6I GCGC 2 cut(s) 55, 79
HinP1I GCGC 2 cut(s) 55, 79
HinfI GANTC 1 cut(s) 230
HpaII CCGG 3 cut(s) 89, 243, 284
HphI GGTGA 1 cut(s) 214
Hpy166II GTNNAC 1 cut(s) 7
Hpy188I TCNGA 2 cut(s) 70, 235
Hpy188III TCNNGA 4 cut(s) 95, 118, 126, 149
Hpy8I GTNNAC 1 cut(s) 7
HpyAV CCTTC 2 cut(s) 155, 290
HpyCH4III ACNGT 1 cut(s) 132
HpyCH4IV ACGT 1 cut(s) 176
HpyCH4V TGCA 2 cut(s) 7, 45
HpyF10VI GCNNNNNNNGC 3 cut(s) 21, 57, 196
HpySE526I ACGT 1 cut(s) 176
Hsp92I GRCGYC 2 cut(s) 55, 176
Hsp92II CATG 1 cut(s) 204
HspAI GCGC 2 cut(s) 55, 79
KasI GGCGCC 1 cut(s) 54
Kzo9I GATC 2 cut(s) 85, 193
LmnI GCTCC 2 cut(s) 216, 245
LpnPI CCDG 8 cut(s) 37, 102, 108, 125, 178, 204, 256, 297
LweI GCATC 1 cut(s) 113
MaeII ACGT 1 cut(s) 176
MalI GATC 2 cut(s) 87, 195
MboI GATC 2 cut(s) 85, 193
MhlI GDGCHC 2 cut(s) 9, 250
MlsI TGGCCA 1 cut(s) 205
MluNI TGGCCA 1 cut(s) 205
Mly113I GGCGCC 1 cut(s) 55
MnlI CCTC 4 cut(s) 91, 118, 190, 195
Mox20I TGGCCA 1 cut(s) 205
MscI TGGCCA 1 cut(s) 205
Msp20I TGGCCA 1 cut(s) 205
MspI CCGG 3 cut(s) 89, 243, 284
MspR9I CCNGG 1 cut(s) 90
MwoI GCNNNNNNNGC 3 cut(s) 21, 57, 196
NarI GGCGCC 1 cut(s) 55
NciI CCSGG 1 cut(s) 90
NcoI CCATGG 1 cut(s) 200
NdeII GATC 2 cut(s) 85, 193
NlaIII CATG 1 cut(s) 204
NlaIV GGNNCC 2 cut(s) 56, 93
NmeAIII GCCGAG 1 cut(s) 166
PfeI GAWTC 1 cut(s) 230
PflMI CCANNNNNTGG 1 cut(s) 37
PkrI GCNGC 1 cut(s) 59
PluTI GGCGCC 1 cut(s) 58
PpuMI RGGWCCY 1 cut(s) 142
Psp5II RGGWCCY 1 cut(s) 142
PspN4I GGNNCC 2 cut(s) 56, 93
PspPI GGNCC 3 cut(s) 92, 142, 240
PspPPI RGGWCCY 1 cut(s) 142
RsaI GTAC 1 cut(s) 159
RsaNI GTAC 1 cut(s) 158
SatI GCNGC 1 cut(s) 58
Sau3AI GATC 2 cut(s) 85, 193
Sau96I GGNCC 3 cut(s) 92, 142, 240
ScrFI CCNGG 1 cut(s) 90
SduI GDGCHC 2 cut(s) 9, 250
SetI ASST 5 cut(s) 38, 102, 144, 179, 275
SfaNI GCATC 1 cut(s) 113
SfoI GGCGCC 1 cut(s) 56
SinI GGWCC 3 cut(s) 92, 142, 240
SsiI CCGC 2 cut(s) 58, 197
SspDI GGCGCC 1 cut(s) 54
StyD4I CCNGG 1 cut(s) 88
StyI CCWWGG 1 cut(s) 200
TaaI ACNGT 1 cut(s) 132
TaiI ACGT 1 cut(s) 179
TaqI TCGA 1 cut(s) 125
TauI GCSGC 1 cut(s) 60
TfiI GAWTC 1 cut(s) 230
Van91I CCANNNNNTGG 1 cut(s) 37
VneI GTGCAC 1 cut(s) 5
VpaK11BI GGWCC 3 cut(s) 92, 142, 240
ZraI GACGTC 1 cut(s) 177
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.