Rh4DG071100

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0020724
Physical Location & Seq
Reverse (-)
10579387 .. 10579701
315 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG071100.1

Sequence Viewer

Length: 315 bp
ATGATTCGCTTCATGGTACACGACTGGTATCTCTGCCACTTCCACAGGTTGTGGTGCAAAGGCCGGCGCCGCCATCAATCGGAGTGTGGCCGCTTTGATCCGGGTCCAGAGGTCGCACCCTCTCATCTCTTGGCTTCGAGACTGTATATTCCGGTCCTTCCCGACTGCGTACAGCCCAGATTTGAGGCGTCCTCGCCTCGGCCTGATCCGCCATGGCCATTTCTCCACCCAATCACCATTGAATCTGATCGGACCGGAGCACCAACCCATCTCTCTCACATCACCTATTTCCTTCCGGCAATAGATGATGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

104

Amino Acids

12.17

Weight (kDa)

6.69

Isoelectric Point (pI)

71.32

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020724)

Species Orthologous Gene IDs
rosa_multiflora Rmu_co8212208.1_g000001 Rmu_sc0000570.1_g000023
rosa_roxburghii Rroxscaffold_2G00099200
rosa_samantha Rh2AG233600 Rh2AG439200 Rh4DG071100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 66
AciI CCGC 3 cut(s) 70, 91, 209
AclWI GGATC 2 cut(s) 92, 200
AcoI YGGCCR 2 cut(s) 88, 215
AcyI GRCGYC 2 cut(s) 67, 188
AfaI GTAC 2 cut(s) 18, 171
AfiI CCNNNNNNNGG 2 cut(s) 79, 198
AgsI TTSAA 1 cut(s) 242
Alw21I GWGCWC 1 cut(s) 262
Alw26I GTCTC 1 cut(s) 133
AlwI GGATC 2 cut(s) 92, 200
AoxI GGCC 4 cut(s) 61, 88, 200, 215
AspLEI GCGC 1 cut(s) 69
AspS9I GGNCC 3 cut(s) 104, 154, 252
AsuC2I CCSGG 1 cut(s) 102
AsuHPI GGTGA 2 cut(s) 226, 274
AvaII GGWCC 3 cut(s) 104, 154, 252
BalI TGGCCA 1 cut(s) 217
BanI GGYRCC 1 cut(s) 66
Bbv12I GWGCWC 1 cut(s) 262
BccI CCATC 2 cut(s) 81, 276
BcnI CCSGG 1 cut(s) 102
BcoDI GTCTC 1 cut(s) 133
BfoI RGCGCY 1 cut(s) 70
BisI GCNGC 2 cut(s) 70, 91
BlsI GCNGC 2 cut(s) 71, 92
Bme1390I CCNGG 1 cut(s) 102
Bme18I GGWCC 3 cut(s) 104, 154, 252
BmgT120I GGNCC 3 cut(s) 104, 154, 252
BmiI GGNNCC 2 cut(s) 68, 105
BmrFI CCNGG 1 cut(s) 102
BplI GAGNNNNNCTC 2 cut(s) 176, 208
BpuMI CCSGG 1 cut(s) 102
BsaHI GRCGYC 2 cut(s) 67, 188
BsaJI CCNNGG 2 cut(s) 197, 212
BsaWI WCCGGW 2 cut(s) 151, 254
Bsc4I CCNNNNNNNGG 2 cut(s) 79, 198
Bse118I RCCGGY 1 cut(s) 63
Bse1I ACTGG 1 cut(s) 29
BseDI CCNNGG 2 cut(s) 197, 212
BseLI CCNNNNNNNGG 2 cut(s) 79, 198
BseNI ACTGG 1 cut(s) 29
BshFI GGCC 4 cut(s) 63, 90, 202, 217
BshNI GGYRCC 1 cut(s) 66
BsiHKAI GWGCWC 1 cut(s) 262
BsiSI CCGG 5 cut(s) 64, 101, 152, 255, 296
BslI CCNNNNNNNGG 2 cut(s) 79, 198
BsmAI GTCTC 1 cut(s) 133
BsnI GGCC 4 cut(s) 63, 90, 202, 217
Bsp1286I GDGCHC 1 cut(s) 262
Bsp143I GATC 3 cut(s) 97, 205, 247
Bsp19I CCATGG 1 cut(s) 212
BspACI CCGC 3 cut(s) 70, 91, 209
BspANI GGCC 4 cut(s) 63, 90, 202, 217
BspLI GGNNCC 2 cut(s) 68, 105
BspPI GGATC 2 cut(s) 92, 200
BspT107I GGYRCC 1 cut(s) 66
BsrFI RCCGGY 1 cut(s) 63
BsrI ACTGG 1 cut(s) 29
BssAI RCCGGY 1 cut(s) 63
BssECI CCNNGG 2 cut(s) 197, 212
BssMI GATC 3 cut(s) 97, 205, 247
BssNI GRCGYC 2 cut(s) 67, 188
BssT1I CCWWGG 1 cut(s) 212
Bst4CI ACNGT 1 cut(s) 144
BstACI GRCGYC 2 cut(s) 67, 188
BstC8I GCNNGC 1 cut(s) 65
BstDSI CCRYGG 1 cut(s) 212
BstH2I RGCGCY 1 cut(s) 70
BstHHI GCGC 1 cut(s) 69
BstKTI GATC 3 cut(s) 100, 208, 250
BstMAI GTCTC 1 cut(s) 133
BstMBI GATC 3 cut(s) 97, 205, 247
BstMWI GCNNNNNNNGC 2 cut(s) 69, 208
BstSCI CCNGG 1 cut(s) 100
BsuRI GGCC 4 cut(s) 63, 90, 202, 217
BtgI CCRYGG 1 cut(s) 212
Cac8I GCNNGC 1 cut(s) 65
CfoI GCGC 1 cut(s) 69
Cfr10I RCCGGY 1 cut(s) 63
Cfr13I GGNCC 3 cut(s) 104, 154, 252
CpoI CGGWCCG 1 cut(s) 252
CseI GACGC 1 cut(s) 177
Csp6I GTAC 2 cut(s) 17, 170
CspI CGGWCCG 1 cut(s) 252
CviAII CATG 2 cut(s) 13, 213
CviJI RGCY 6 cut(s) 63, 90, 134, 175, 202, 217
CviKI_1 RGCY 6 cut(s) 63, 90, 134, 175, 202, 217
CviQI GTAC 2 cut(s) 17, 170
DinI GGCGCC 1 cut(s) 68
DpnI GATC 3 cut(s) 99, 207, 249
DpnII GATC 3 cut(s) 97, 205, 247
EaeI YGGCCR 2 cut(s) 88, 215
EciI GGCGGA 1 cut(s) 198
Eco130I CCWWGG 1 cut(s) 212
Eco47I GGWCC 3 cut(s) 104, 154, 252
EcoT14I CCWWGG 1 cut(s) 212
EgeI GGCGCC 1 cut(s) 68
EheI GGCGCC 1 cut(s) 68
ErhI CCWWGG 1 cut(s) 212
FaeI CATG 2 cut(s) 16, 216
FaiI YATR 3 cut(s) 14, 147, 214
FatI CATG 2 cut(s) 12, 212
Fnu4HI GCNGC 2 cut(s) 70, 91
Fsp4HI GCNGC 2 cut(s) 70, 91
GlaI GCGC 1 cut(s) 68
GluI GCNGC 2 cut(s) 70, 91
HaeII RGCGCY 1 cut(s) 70
HaeIII GGCC 4 cut(s) 63, 90, 202, 217
HapII CCGG 5 cut(s) 64, 101, 152, 255, 296
HgaI GACGC 1 cut(s) 177
HhaI GCGC 1 cut(s) 69
Hin1I GRCGYC 2 cut(s) 67, 188
Hin1II CATG 2 cut(s) 16, 216
Hin6I GCGC 1 cut(s) 67
HinP1I GCGC 1 cut(s) 67
HinfI GANTC 2 cut(s) 4, 242
HpaII CCGG 5 cut(s) 64, 101, 152, 255, 296
HphI GGTGA 2 cut(s) 226, 274
Hpy166II GTNNAC 1 cut(s) 19
Hpy188I TCNGA 3 cut(s) 82, 247, 252
Hpy188III TCNNGA 3 cut(s) 107, 138, 161
Hpy8I GTNNAC 1 cut(s) 19
HpyAV CCTTC 2 cut(s) 167, 302
HpyCH4III ACNGT 1 cut(s) 144
HpyCH4V TGCA 1 cut(s) 57
HpyF10VI GCNNNNNNNGC 2 cut(s) 69, 208
Hsp92I GRCGYC 2 cut(s) 67, 188
Hsp92II CATG 2 cut(s) 16, 216
HspAI GCGC 1 cut(s) 67
KasI GGCGCC 1 cut(s) 66
KroI GCCGGC 1 cut(s) 63
KroNI GCCGGC 1 cut(s) 65
Kzo9I GATC 3 cut(s) 97, 205, 247
LmnI GCTCC 1 cut(s) 257
MalI GATC 3 cut(s) 99, 207, 249
MboI GATC 3 cut(s) 97, 205, 247
MhlI GDGCHC 1 cut(s) 262
MlsI TGGCCA 1 cut(s) 217
MluNI TGGCCA 1 cut(s) 217
Mly113I GGCGCC 1 cut(s) 67
MnlI CCTC 5 cut(s) 103, 130, 178, 202, 207
Mox20I TGGCCA 1 cut(s) 217
MroNI GCCGGC 1 cut(s) 63
MscI TGGCCA 1 cut(s) 217
Msp20I TGGCCA 1 cut(s) 217
MspI CCGG 5 cut(s) 64, 101, 152, 255, 296
MspR9I CCNGG 1 cut(s) 102
MwoI GCNNNNNNNGC 2 cut(s) 69, 208
NaeI GCCGGC 1 cut(s) 65
NarI GGCGCC 1 cut(s) 67
NciI CCSGG 1 cut(s) 102
NcoI CCATGG 1 cut(s) 212
NdeII GATC 3 cut(s) 97, 205, 247
NgoMIV GCCGGC 1 cut(s) 63
NlaIII CATG 2 cut(s) 16, 216
NlaIV GGNNCC 2 cut(s) 68, 105
NmeAIII GCCGAG 1 cut(s) 178
PdiI GCCGGC 1 cut(s) 65
PfeI GAWTC 2 cut(s) 4, 242
PkrI GCNGC 2 cut(s) 71, 92
PluTI GGCGCC 1 cut(s) 70
PspN4I GGNNCC 2 cut(s) 68, 105
PspPI GGNCC 3 cut(s) 104, 154, 252
RsaI GTAC 2 cut(s) 18, 171
RsaNI GTAC 2 cut(s) 17, 170
Rsr2I CGGWCCG 1 cut(s) 252
RsrII CGGWCCG 1 cut(s) 252
SatI GCNGC 2 cut(s) 70, 91
Sau3AI GATC 3 cut(s) 97, 205, 247
Sau96I GGNCC 3 cut(s) 104, 154, 252
ScrFI CCNGG 1 cut(s) 102
SduI GDGCHC 1 cut(s) 262
SetI ASST 3 cut(s) 50, 114, 287
SfoI GGCGCC 1 cut(s) 68
SinI GGWCC 3 cut(s) 104, 154, 252
SsiI CCGC 3 cut(s) 70, 91, 209
SspDI GGCGCC 1 cut(s) 66
StyD4I CCNGG 1 cut(s) 100
StyI CCWWGG 1 cut(s) 212
TaaI ACNGT 1 cut(s) 144
TaqI TCGA 1 cut(s) 137
TauI GCSGC 2 cut(s) 72, 93
TfiI GAWTC 2 cut(s) 4, 242
VpaK11BI GGWCC 3 cut(s) 104, 154, 252
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.