Rh2CG061500

divergent subfamily of APPLE domains

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0018769
Physical Location & Seq
Forward (+)
5026415 .. 5028847
2433 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG061500.1

Sequence Viewer

Length: 1182 bp
ATGCATCTCAATAAGATTTTTATCTCGATGGAAGTAGAAGAAGAAGAAGAAGAAGTTGCACATCTCCCCCAAACTTCATCAGAAGTTGCACAAGTGGCCACCACCTTCTCTAAATTTGAAGAGCTAGAGTCCATCTCTGATGAGATTCGATTATGGCTTCCAAGAGAAAAGTTGTTCCAATGCAAGGTGGTGTCAAAGGAGTGGCTTAAGATGATCACAGATGAGTTTTTTATGCGAAGGTTTGAAGCACGGCAAGATATCAACTCGAGCATCTTCTTGGTTGATCTGGAAAGACCTGAAGTAGTGACAATCAAGTTTGATGAGGAAGCAAAATATTGTGTCGAACACTTCAAAGCCAGTGCTCTAAAAGGTATGAAGTGCCCTAGCATATTAGGTTCTGCTGGTGGCCTAATATGTATAGGCTTAGGGGATGAGCATGTGCACCAGACAATTGTAGTCATCAATCCCAGGACAAGAATGTGTAGGAAGATTGTTGAAGAAGAGTCATTACCTGGCTTCGTGGTGGGAGGATCATATGCCTTTGGCTATGAAGAAGACAAAAAGGAGCACACCATAGTGAAGATATACACCATCATTGGGGATGGTGAAGACAAGACTTGTGTCAATATGTTTTCACTGGGAGACATCAAATGGAGGGTGAAACACTTCCCTCATTTCCAGACTCAAACTGTGTTGACTAAAGCTGTTGGATCCTACAATGAATACATTTGTTGGCATGCATCAGAGGAAGGAAAGCTAATAACTTTCAACCTCAAAACTGAGGCCCTGGAGACAATAGAGCTTCCGCTTAATCTTCAAGACAGAGATGTTAGCTTCAGTGTGATGCACCAGGTTGTTGGTGGGAAACTATGTGAAGAACTTATAATATGCTGCCCCCGGGAGGGTGGAACAGAGCTTTGGAGGTTAGACATGAATGATAATGTGTTCAAGAGAATAAGAACTGTGATGCACAATGGGCCATTAGAGCCTTTATGCTATTTGTTGGGCGATGAAAAAGAAATTGTTGTGAAGGGTGCACGCAGCATAGAATTATACCGTTCGGACAACTCCATAATGCCCATATATGATGCCAGCAACGATAAAAGAAAGAAGATTGCAGCAATCGGCTTCTGTGAAAATTCGATCTCTCCTGGACTAGATCCAAAGACGGAAGAGGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

393

Amino Acids

44.87

Weight (kDa)

5.18

Isoelectric Point (pI)

44.61

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018769)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g05570 FvH4_3g15970 FvH4_3g15970
rosa_roxburghii Rroxscaffold_2G00150330
rosa_samantha Rh2AG060700 Rh2BG059600 Rh2CG061500 Rh2DG060000
rosa_wichuraiana Rw2G004920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 884
AciI CCGC 1 cut(s) 806
AclWI GGATC 4 cut(s) 538, 705, 718, 1154
AcoI YGGCCR 1 cut(s) 96
AcsI RAATTY 2 cut(s) 113, 1138
AcuI CTGAAG 2 cut(s) 318, 820
AfiI CCNNNNNNNGG 4 cut(s) 184, 597, 901, 902
AflII CTTAAG 1 cut(s) 206
AgsI TTSAA 7 cut(s) 119, 245, 352, 497, 769, 818, 949
AjnI CCWGG 5 cut(s) 467, 511, 786, 849, 1150
AjuI GAANNNNNNNTTGG 2 cut(s) 901, 933
AleI CACNNNNGTG 1 cut(s) 575
AluBI AGCT 6 cut(s) 124, 704, 757, 802, 834, 916
AluI AGCT 6 cut(s) 124, 704, 757, 802, 834, 916
Alw21I GWGCWC 4 cut(s) 364, 444, 570, 1039
Alw26I GTCTC 2 cut(s) 636, 785
Alw44I GTGCAC 2 cut(s) 440, 1035
AlwI GGATC 4 cut(s) 538, 705, 718, 1154
Ama87I CYCGRG 2 cut(s) 265, 897
AoxI GGCC 4 cut(s) 96, 406, 783, 977
ApaLI GTGCAC 2 cut(s) 440, 1035
ApeKI GCWGC 3 cut(s) 891, 1041, 1118
ApoI RAATTY 2 cut(s) 113, 1138
Asp700I GAANNNNTTC 1 cut(s) 665
AspS9I GGNCC 2 cut(s) 784, 977
AsuC2I CCSGG 2 cut(s) 898, 899
AsuHPI GGTGA 2 cut(s) 617, 670
AvaI CYCGRG 2 cut(s) 265, 897
BaeGI GKGCMC 3 cut(s) 383, 444, 1039
BalI TGGCCA 1 cut(s) 98
BamHI GGATCC 1 cut(s) 710
BarI GAAGNNNNNNTAC 2 cut(s) 492, 524
BbsI GAAGAC 2 cut(s) 561, 615
Bbv12I GWGCWC 4 cut(s) 364, 444, 570, 1039
BbvI GCAGC 3 cut(s) 878, 1053, 1130
BccI CCATC 4 cut(s) 22, 140, 596, 599
BceAI ACGGC 1 cut(s) 266
BciT130I CCWGG 5 cut(s) 469, 513, 788, 851, 1152
BclI TGATCA 1 cut(s) 213
BcnI CCSGG 2 cut(s) 898, 899
BcoDI GTCTC 2 cut(s) 636, 785
BfaI CTAG 3 cut(s) 125, 384, 1157
BfrI CTTAAG 1 cut(s) 206
BisI GCNGC 3 cut(s) 892, 1042, 1119
BlsI GCNGC 3 cut(s) 893, 1043, 1120
Bme1390I CCNGG 7 cut(s) 469, 513, 788, 851, 898, 899, 1152
BmeT110I CYCGRG 2 cut(s) 265, 897
BmgT120I GGNCC 2 cut(s) 784, 977
BmiI GGNNCC 1 cut(s) 712
BmrFI CCNGG 7 cut(s) 469, 513, 788, 851, 898, 899, 1152
BmrI ACTGGG 1 cut(s) 647
BmsI GCATC 6 cut(s) 13, 279, 749, 834, 957, 1078
BmuI ACTGGG 1 cut(s) 647
BoxI GACNNNNGTC 1 cut(s) 620
BpiI GAAGAC 2 cut(s) 561, 615
BplI GAGNNNNNCTC 2 cut(s) 119, 151
BpmI CTGGAG 1 cut(s) 809
Bpu10I CCTNAGC 1 cut(s) 424
BpuMI CCSGG 2 cut(s) 898, 899
BsaBI GATNNNNATC 1 cut(s) 20
BsaJI CCNNGG 4 cut(s) 467, 786, 896, 897
Bsc4I CCNNNNNNNGG 4 cut(s) 184, 597, 901, 902
Bse1I ACTGG 2 cut(s) 357, 642
Bse8I GATNNNNATC 1 cut(s) 20
BseBI CCWGG 5 cut(s) 469, 513, 788, 851, 1152
BseDI CCNNGG 4 cut(s) 467, 786, 896, 897
BseGI GGATG 2 cut(s) 436, 607
BseJI GATNNNNATC 1 cut(s) 20
BseLI CCNNNNNNNGG 4 cut(s) 184, 597, 901, 902
BseMII CTCAG 1 cut(s) 771
BseNI ACTGG 2 cut(s) 357, 642
BseSI GKGCMC 3 cut(s) 383, 444, 1039
BseXI GCAGC 3 cut(s) 878, 1053, 1130
BshFI GGCC 4 cut(s) 98, 408, 785, 979
BsiHKAI GWGCWC 4 cut(s) 364, 444, 570, 1039
BsiHKCI CYCGRG 2 cut(s) 265, 897
BsiSI CCGG 1 cut(s) 898
BslI CCNNNNNNNGG 4 cut(s) 184, 597, 901, 902
BsmAI GTCTC 2 cut(s) 636, 785
BsnI GGCC 4 cut(s) 98, 408, 785, 979
BsoBI CYCGRG 2 cut(s) 265, 897
Bsp1286I GDGCHC 5 cut(s) 364, 383, 444, 570, 1039
Bsp143I GATC 6 cut(s) 213, 283, 530, 710, 1143, 1159
BspACI CCGC 1 cut(s) 806
BspANI GGCC 4 cut(s) 98, 408, 785, 979
BspCNI CTCAG 1 cut(s) 772
BspLI GGNNCC 1 cut(s) 712
BspPI GGATC 4 cut(s) 538, 705, 718, 1154
BspQI GCTCTTC 1 cut(s) 114
BspTI CTTAAG 1 cut(s) 206
BsrI ACTGG 2 cut(s) 357, 642
BssECI CCNNGG 4 cut(s) 467, 786, 896, 897
BssMI GATC 6 cut(s) 213, 283, 530, 710, 1143, 1159
Bst2UI CCWGG 5 cut(s) 469, 513, 788, 851, 1152
Bst4CI ACNGT 3 cut(s) 691, 964, 1058
Bst6I CTCTTC 3 cut(s) 114, 495, 1167
BstAFI CTTAAG 1 cut(s) 206
BstC8I GCNNGC 3 cut(s) 738, 1039, 1093
BstDEI CTNAG 2 cut(s) 424, 780
BstF5I GGATG 2 cut(s) 436, 607
BstKTI GATC 6 cut(s) 216, 286, 533, 713, 1146, 1162
BstMAI GTCTC 2 cut(s) 636, 785
BstMBI GATC 6 cut(s) 213, 283, 530, 710, 1143, 1159
BstMWI GCNNNNNNNGC 3 cut(s) 95, 976, 985
BstNI CCWGG 5 cut(s) 469, 513, 788, 851, 1152
BstNSI RCATGY 2 cut(s) 440, 740
BstPAI GACNNNNGTC 1 cut(s) 620
BstSCI CCNGG 7 cut(s) 467, 511, 786, 849, 896, 897, 1150
BstSLI GKGCMC 3 cut(s) 383, 444, 1039
BstV1I GCAGC 3 cut(s) 878, 1053, 1130
BstV2I GAAGAC 2 cut(s) 561, 615
BstX2I RGATCY 2 cut(s) 710, 1159
BstXI CCANNNNNNTGG 1 cut(s) 857
BstYI RGATCY 2 cut(s) 710, 1159
BsuRI GGCC 4 cut(s) 98, 408, 785, 979
BtgZI GCGATG 1 cut(s) 1023
BtsCI GGATG 2 cut(s) 436, 607
BtsIMutI CAGTG 3 cut(s) 364, 635, 844
Cac8I GCNNGC 3 cut(s) 738, 1039, 1093
Cfr13I GGNCC 2 cut(s) 784, 977
Cfr9I CCCGGG 1 cut(s) 897
CsiI ACCWGGT 1 cut(s) 849
CviAII CATG 3 cut(s) 437, 737, 931
DdeI CTNAG 2 cut(s) 424, 780
DpnI GATC 6 cut(s) 215, 285, 532, 712, 1145, 1161
DpnII GATC 6 cut(s) 213, 283, 530, 710, 1143, 1159
EaeI YGGCCR 1 cut(s) 96
Eam1104I CTCTTC 3 cut(s) 114, 495, 1167
EarI CTCTTC 3 cut(s) 114, 495, 1167
Eco32I GATATC 1 cut(s) 259
Eco57I CTGAAG 2 cut(s) 318, 820
Eco88I CYCGRG 2 cut(s) 265, 897
EcoO109I RGGNCCY 1 cut(s) 784
EcoRII CCWGG 5 cut(s) 467, 511, 786, 849, 1150
EcoRV GATATC 1 cut(s) 259
EcoT22I ATGCAT 2 cut(s) 6, 742
FaeI CATG 3 cut(s) 440, 740, 934
FatI CATG 3 cut(s) 436, 736, 930
FauNDI CATATG 1 cut(s) 535
FbaI TGATCA 1 cut(s) 213
Fnu4HI GCNGC 3 cut(s) 892, 1042, 1119
FokI GGATG 2 cut(s) 443, 614
Fsp4HI GCNGC 3 cut(s) 892, 1042, 1119
FspBI CTAG 3 cut(s) 125, 384, 1157
GluI GCNGC 3 cut(s) 892, 1042, 1119
GsuI CTGGAG 1 cut(s) 809
HaeIII GGCC 4 cut(s) 98, 408, 785, 979
HapII CCGG 1 cut(s) 898
Hin1II CATG 3 cut(s) 440, 740, 934
HincII GTYRAC 1 cut(s) 696
HindII GTYRAC 1 cut(s) 696
HinfI GANTC 4 cut(s) 128, 145, 503, 682
HpaII CCGG 1 cut(s) 898
HphI GGTGA 2 cut(s) 617, 670
Hpy166II GTNNAC 3 cut(s) 442, 696, 1037
Hpy188I TCNGA 4 cut(s) 82, 139, 745, 1063
Hpy188III TCNNGA 5 cut(s) 25, 287, 679, 818, 949
Hpy8I GTNNAC 3 cut(s) 442, 696, 1037
HpyAV CCTTC 4 cut(s) 115, 231, 743, 1024
HpyCH4III ACNGT 3 cut(s) 691, 964, 1058
HpyF10VI GCNNNNNNNGC 3 cut(s) 95, 976, 985
HpyF3I CTNAG 2 cut(s) 424, 780
Hsp92II CATG 3 cut(s) 440, 740, 934
Ksp22I TGATCA 1 cut(s) 213
Kzo9I GATC 6 cut(s) 213, 283, 530, 710, 1143, 1159
LguI GCTCTTC 1 cut(s) 114
LmnI GCTCC 1 cut(s) 565
Lsp1109I GCAGC 3 cut(s) 878, 1053, 1130
LweI GCATC 6 cut(s) 13, 279, 749, 834, 957, 1078
MabI ACCWGGT 1 cut(s) 849
MaeI CTAG 3 cut(s) 125, 384, 1157
MaeIII GTNAC 1 cut(s) 304
MalI GATC 6 cut(s) 215, 285, 532, 712, 1145, 1161
MboI GATC 6 cut(s) 213, 283, 530, 710, 1143, 1159
MfeI CAATTG 1 cut(s) 450
MflI RGATCY 2 cut(s) 710, 1159
MhlI GDGCHC 5 cut(s) 364, 383, 444, 570, 1039
MlsI TGGCCA 1 cut(s) 98
MluCI AATT 5 cut(s) 113, 450, 1020, 1049, 1138
MluNI TGGCCA 1 cut(s) 98
MlyI GAGTC 3 cut(s) 137, 512, 676
MmeI TCCRAC 1 cut(s) 688
Mox20I TGGCCA 1 cut(s) 98
Mph1103I ATGCAT 2 cut(s) 6, 742
MroXI GAANNNNTTC 1 cut(s) 665
MscI TGGCCA 1 cut(s) 98
MseI TTAA 2 cut(s) 207, 810
MslI CAYNNNNRTG 1 cut(s) 575
Msp20I TGGCCA 1 cut(s) 98
MspCI CTTAAG 1 cut(s) 206
MspI CCGG 1 cut(s) 898
MspR9I CCNGG 7 cut(s) 469, 513, 788, 851, 898, 899, 1152
MunI CAATTG 1 cut(s) 450
MvaI CCWGG 5 cut(s) 469, 513, 788, 851, 1152
MwoI GCNNNNNNNGC 3 cut(s) 95, 976, 985
NciI CCSGG 2 cut(s) 898, 899
NdeI CATATG 1 cut(s) 535
NdeII GATC 6 cut(s) 213, 283, 530, 710, 1143, 1159
NlaIII CATG 3 cut(s) 440, 740, 934
NlaIV GGNNCC 1 cut(s) 712
NmuCI GTSAC 1 cut(s) 304
NsiI ATGCAT 2 cut(s) 6, 742
NspI RCATGY 2 cut(s) 440, 740
OliI CACNNNNGTG 1 cut(s) 575
PaeI GCATGC 1 cut(s) 740
PaeR7I CTCGAG 1 cut(s) 265
PciSI GCTCTTC 1 cut(s) 114
PdmI GAANNNNTTC 1 cut(s) 665
PfeI GAWTC 1 cut(s) 145
PfoI TCCNGGA 1 cut(s) 1150
PkrI GCNGC 3 cut(s) 893, 1043, 1120
PleI GAGTC 3 cut(s) 136, 511, 676
PpsI GAGTC 3 cut(s) 136, 511, 676
PshAI GACNNNNGTC 1 cut(s) 620
PsiI TTATAA 1 cut(s) 884
Psp6I CCWGG 5 cut(s) 467, 511, 786, 849, 1150
PspGI CCWGG 5 cut(s) 467, 511, 786, 849, 1150
PspN4I GGNNCC 1 cut(s) 712
PspPI GGNCC 2 cut(s) 784, 977
PspXI VCTCGAGB 1 cut(s) 265
PsuI RGATCY 2 cut(s) 710, 1159
RseI CAYNNNNRTG 1 cut(s) 575
SapI GCTCTTC 1 cut(s) 114
SaqAI TTAA 2 cut(s) 207, 810
SatI GCNGC 3 cut(s) 892, 1042, 1119
Sau3AI GATC 6 cut(s) 213, 283, 530, 710, 1143, 1159
Sau96I GGNCC 2 cut(s) 784, 977
SchI GAGTC 3 cut(s) 137, 512, 676
ScrFI CCNGG 7 cut(s) 469, 513, 788, 851, 898, 899, 1152
SduI GDGCHC 5 cut(s) 364, 383, 444, 570, 1039
SexAI ACCWGGT 1 cut(s) 849
SfaNI GCATC 6 cut(s) 13, 279, 749, 834, 957, 1078
Sfr274I CTCGAG 1 cut(s) 265
SlaI CTCGAG 1 cut(s) 265
SmaI CCCGGG 1 cut(s) 899
SmiMI CAYNNNNRTG 1 cut(s) 575
SmlI CTYRAG 2 cut(s) 206, 265
SmoI CTYRAG 2 cut(s) 206, 265
SphI GCATGC 1 cut(s) 740
Sse9I AATT 5 cut(s) 113, 450, 1020, 1049, 1138
SsiI CCGC 1 cut(s) 806
SspI AATATT 1 cut(s) 335
SspMI CTAG 3 cut(s) 125, 384, 1157
StyD4I CCNGG 7 cut(s) 467, 511, 786, 849, 896, 897, 1150
TaaI ACNGT 3 cut(s) 691, 964, 1058
TaqI TCGA 5 cut(s) 26, 148, 266, 342, 1142
TasI AATT 5 cut(s) 113, 450, 1020, 1049, 1138
TfiI GAWTC 1 cut(s) 145
Tru1I TTAA 2 cut(s) 207, 810
Tru9I TTAA 2 cut(s) 207, 810
TscAI CASTG 3 cut(s) 364, 642, 844
TseFI GTSAC 1 cut(s) 304
TseI GCWGC 3 cut(s) 891, 1041, 1118
Tsp45I GTSAC 1 cut(s) 304
TspDTI ATGAA 6 cut(s) 66, 389, 564, 735, 947, 1026
TspMI CCCGGG 1 cut(s) 897
TspRI CASTG 3 cut(s) 364, 642, 844
Vha464I CTTAAG 1 cut(s) 206
VneI GTGCAC 2 cut(s) 440, 1035
XapI RAATTY 2 cut(s) 113, 1138
XceI RCATGY 2 cut(s) 440, 740
XcmI CCANNNNNNNNNTGG 1 cut(s) 857
XhoI CTCGAG 1 cut(s) 265
XmaI CCCGGG 1 cut(s) 897
XmnI GAANNNNTTC 1 cut(s) 665
XspI CTAG 3 cut(s) 125, 384, 1157
Zsp2I ATGCAT 2 cut(s) 6, 742
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.