Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr2C

Orthogroup ID

OG0013338

Physical Location & Seq

Genomic Coordinates Forward (+)

61010706 .. 61013372

Gene Structure 2667 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh2CG449200.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1692 bp

ATGGACCGGCCGGACTCCTCCTCTTCCGACGACGACGACGATCGCCAGAACCTAATCGAACAGAACGAGCGCAAGCATCAGCAGCTCCCCAGCCCTCGATCCGCCACCACTTTCCAAATCGACGACGTCAACGTCGACCACCACCGCCACCACCGCGAAATACGACGGCGTTTCGCCTCCCTCAAGCTCAGCGACTTGTTCAACAAACGCTTCCTCATCTTCTTCATCTTCATCCCTCTCTTCGTCCTCATCCTCTTCTTCACCACCGACATCAAGAGCCTCTTCTTCTCCCACCTCTCTGTGCCTCTCTCCGATTCCCTCTCCGATAAGCTCCGCGAGTCCGAACTGCGAGCTCTCTTCTTGCTCCACCAGCAGCAGCTCGGTCTCTTCGGCCTCTGGAACCAAACGCATCCCAATTCCACTTCAAATCACTCCAATTCCGAACTCCAGGATTTCAAATCCTCGATTTTGAAGCAGATTTCGTTGAATAAGGAGATTCAGCAGGTGTTGTTATCTCCGCACAGCTCTGGCAACTCATCATCGGAATCCGTAGATTTCCGGGATCCGAGCCTCGGCGATCGGTGCGGATTAGTGGACCAGCGGTTTTCGGAGAGAAGAACAATTGACTGGAAGCCTAATTCCGATAAGTACTTGTTGGCGATTTGCGTTTCGGGCCAAATGTCCAACCATTTGATCTGCTTGGAGAAGCACATGTTCTTCGCAGCATTGCTGAACCGGGTTTTGGTGATTCCTAGCCCTAAAGTGGATTACCAGTACAGCACTGTGTTGGATATTGAGCACATTAACAAATGCATAGGCAGAAAAGTTGTGGTTACTTTCGAGGAATTGGCCGAGGAGAAGAAGAATCACATCCACATTGATAGGTTTATTTGCTATTTTTCGAAGCCGACGCTTTGCTATGTGGATGATGAGCATCTCAAGAAGCTGAAGGCATTGGGAATTTCGTTCAAGAGTCGTGAGCCTGCTTGGGGGGAAGATGTGAAGAAGCCGAGCAAGAAGACTGTGCAGGATGTTCAGTCCAAGTTTTCGAGTGGTGATGATGTTATTGCCATTGGGGATGTTTTCTTTGCTGATTTGGAGAAAGATTGGGTGATGCAGCCGGGTGGTCCTCTAGCTCACAAATGCAAGACTCTCGTTGAGCCAAGTCGGCTTATAATGCTAACTGCCCAGCGTTTCATTCAGACGTTCCTGGGGAAGAATTTCATTGCACTACATTTCCGGCGGCATGGCTTCTTGAAGTTCTGCAATAATAAGCAGCCAAGTTGCTTCTACCCCATTCCGCAAGCCGCGGATTGCATCACCCGGGTTGCTGAAAGGGCCAATGCACCAGTTATATATCTTTCAACAGATGCAGCTGAAAGCGAAACTGGTTTACTGCAGTCACTAGTTGTTGCGAATGGGAAGGCTGTACCACTTGTTAAACGGCCTGCACGAAATTCAGCTGAAAAGTGGGATGCTTTGTTATACAGGCATGGAATTGAGGGGGACTCTCAGGTAGAAGCTATGCTGGATAAGACAATAAGTGCCATGTCAAGTGTTTTCATCGGAGCATCTGGATCCACTTTCACAGAGGACATCTTGCGCCTCAGGAAGGATTGGGGTTCAGCTTCTGTGTGTGATGAGTACCTCTGCCAAGGTGAAGAGCCTAACTTCATTGCAGAGAATGAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0006464 GO:0006486 GO:0006493 GO:0006807 GO:0008150 GO:0008152 GO:0008417 GO:0009058 GO:0009059 GO:0009100 GO:0009101 GO:0009987 GO:0016740 GO:0016757 GO:0016758 GO:0019538 GO:0034645 GO:0036065 GO:0036066 GO:0036211 GO:0043170 GO:0043412 GO:0043413 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0046922 GO:0070085 GO:0071704 GO:0140096 GO:1901135 GO:1901137 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00514 map00514

Pfam Domains

Protein Families

O-FucT

Protein Analysis

563

Amino Acids

64.1

Weight (kDa)

6.59

Isoelectric Point (pI)

48.07

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
O-FucT	PF10250	236 - 537	1.3e-07	GDP-fucose protein O-fucosyltransferase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0013338)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G50420
fragaria_vesca	FvH4_6g36080
malus_domestica	MD09G1171900.v1.1 MD17G1155100.v1.1
prunus_persica	Prupe.3G030300_v2.0.a1
pyrus_communis	pycom09g08920 pycom17g14790
rosa_chinensis	RchiOBHm_Chr2g0148131
rosa_laevigata	RLG00000020319
rosa_multiflora	Rmu_co8472331.1_g000001 Rmu_sc0001516.1_g000096 Rmu_sc0012028.1_g000005
rosa_rugosa	Rorug02G0403900
rosa_samantha	Rh2AG462400 Rh2BG475400 Rh2CG449200 Rh2DG484100
rosa_wichuraiana	Rw2G037690

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1175
AarI	CACCTGC	1 cut(s)	493
AasI	GACNNNNNNGTC	1 cut(s)	131
AatII	GACGTC	1 cut(s)	129
Acc36I	ACCTGC	1 cut(s)	493
AccI	GTMKAC	1 cut(s)	135
AccII	CGCG	3 cut(s)	156, 336, 1310
AclWI	GGATC	5 cut(s)	93, 557, 570, 1572, 1585
AcoI	YGGCCR	2 cut(s)	8, 849
AcsI	RAATTY	3 cut(s)	960, 1219, 1456
AcuI	CTGAAG	1 cut(s)	968
AcyI	GRCGYC	1 cut(s)	126
AfaI	GTAC	4 cut(s)	650, 776, 1431, 1646
AfiI	CCNNNNNNNGG	5 cut(s)	572, 742, 763, 989, 1612
AflIII	ACRYGT	1 cut(s)	711
AgsI	TTSAA	8 cut(s)	202, 426, 457, 472, 487, 970, 1258, 1365
AhlI	ACTAGT	1 cut(s)	1405
AjnI	CCWGG	2 cut(s)	447, 1209
AjuI	GAANNNNNNNTTGG	2 cut(s)	725, 757
Alw21I	GWGCWC	2 cut(s)	355, 801
Alw26I	GTCTC	1 cut(s)	389
AlwI	GGATC	5 cut(s)	93, 557, 570, 1572, 1585
AlwNI	CAGNNNCTG	1 cut(s)	1631
Ama87I	CYCGRG	1 cut(s)	1323
AoxI	GGCC	6 cut(s)	8, 391, 673, 849, 1338, 1445
ApeKI	GCWGC	7 cut(s)	82, 373, 376, 722, 1117, 1276, 1373
ApoI	RAATTY	3 cut(s)	960, 1219, 1456
Asp700I	GAANNNNTTC	1 cut(s)	1220
AspLEI	GCGC	2 cut(s)	72, 1605
AspS9I	GGNCC	5 cut(s)	4, 595, 673, 1127, 1338
AsuC2I	CCSGG	5 cut(s)	560, 737, 1122, 1324, 1325
AsuHPI	GGTGA	6 cut(s)	253, 757, 1067, 1123, 1312, 1670
AsuII	TTCGAA	1 cut(s)	902
AvaI	CYCGRG	1 cut(s)	1323
AvaII	GGWCC	3 cut(s)	4, 595, 1127
AxyI	CCTNAGG	1 cut(s)	1607
BamHI	GGATCC	2 cut(s)	562, 1577
BanII	GRGCYC	1 cut(s)	355
BbsI	GAAGAC	1 cut(s)	1025
Bbv12I	GWGCWC	2 cut(s)	355, 801
BbvI	GCAGC	7 cut(s)	94, 385, 388, 734, 1129, 1288, 1385
BceAI	ACGGC	2 cut(s)	182, 1460
BcgI	CGANNNNNNTGC	2 cut(s)	1135, 1169
BciT130I	CCWGG	2 cut(s)	449, 1211
BcnI	CCSGG	5 cut(s)	560, 737, 1122, 1324, 1325
BcoDI	GTCTC	1 cut(s)	389
BcuI	ACTAGT	1 cut(s)	1405
BfaI	CTAG	3 cut(s)	753, 1133, 1406
BfmI	CTRYAG	1 cut(s)	1397
BfuAI	ACCTGC	1 cut(s)	493
BglI	GCCNNNNNGGC	1 cut(s)	1168
BisI	GCNGC	9 cut(s)	83, 374, 377, 723, 1118, 1244, 1277, 1308, 1374
BlpI	GCTNAGC	1 cut(s)	188
BlsI	GCNGC	9 cut(s)	84, 375, 378, 724, 1119, 1245, 1278, 1309, 1375
BmcAI	AGTACT	1 cut(s)	650
Bme1390I	CCNGG	7 cut(s)	449, 560, 737, 1122, 1211, 1324, 1325
Bme18I	GGWCC	3 cut(s)	4, 595, 1127
BmeT110I	CYCGRG	1 cut(s)	1323
BmgT120I	GGNCC	5 cut(s)	4, 595, 673, 1127, 1338
BmiI	GGNNCC	3 cut(s)	401, 564, 1579
BmrFI	CCNGG	7 cut(s)	449, 560, 737, 1122, 1211, 1324, 1325
BmsI	GCATC	8 cut(s)	85, 418, 943, 1104, 1326, 1360, 1465, 1580
BpiI	GAAGAC	1 cut(s)	1025
BplI	GAGNNNNNCTC	2 cut(s)	1493, 1525
BpmI	CTGGAG	1 cut(s)	431
Bpu1102I	GCTNAGC	1 cut(s)	188
Bpu14I	TTCGAA	1 cut(s)	902
BpuEI	CTTGAG	2 cut(s)	167, 923
BpuMI	CCSGG	5 cut(s)	560, 737, 1122, 1324, 1325
BsaBI	GATNNNNATC	1 cut(s)	933
BsaHI	GRCGYC	1 cut(s)	126
BsaI	GGTCTC	1 cut(s)	389
BsaJI	CCNNGG	6 cut(s)	571, 852, 1210, 1308, 1323, 1654
Bsc4I	CCNNNNNNNGG	5 cut(s)	572, 742, 763, 989, 1612
Bse118I	RCCGGY	1 cut(s)	6
Bse1I	ACTGG	4 cut(s)	632, 772, 1349, 1393
Bse21I	CCTNAGG	1 cut(s)	1607
Bse3DI	GCAATG	3 cut(s)	725, 1224, 1674
Bse8I	GATNNNNATC	1 cut(s)	933
BseBI	CCWGG	2 cut(s)	449, 1211
BseDI	CCNNGG	6 cut(s)	571, 852, 1210, 1308, 1323, 1654
BseGI	GGATG	8 cut(s)	231, 249, 409, 870, 931, 1036, 1084, 1480
BseJI	GATNNNNATC	1 cut(s)	933
BseLI	CCNNNNNNNGG	5 cut(s)	572, 742, 763, 989, 1612
BseMI	GCAATG	3 cut(s)	725, 1224, 1674
BseMII	CTCAG	3 cut(s)	202, 1526, 1621
BseNI	ACTGG	4 cut(s)	632, 772, 1349, 1393
BseRI	GAGGAG	3 cut(s)	7, 10, 869
BseX3I	CGGCCG	1 cut(s)	8
BseXI	GCAGC	7 cut(s)	94, 385, 388, 734, 1129, 1288, 1385
BseYI	CCCAGC	2 cut(s)	89, 1188
BsgI	GTGCAG	2 cut(s)	1046, 1434
Bsh1236I	CGCG	3 cut(s)	156, 336, 1310
Bsh1285I	CGRYCG	3 cut(s)	11, 43, 580
BshFI	GGCC	6 cut(s)	10, 393, 675, 851, 1340, 1447
BsiEI	CGRYCG	3 cut(s)	11, 43, 580
BsiHKAI	GWGCWC	2 cut(s)	355, 801
BsiHKCI	CYCGRG	1 cut(s)	1323
BsiSI	CCGG	7 cut(s)	7, 11, 559, 736, 1121, 1240, 1324
BslFI	GGGAC	1 cut(s)	1520
BslI	CCNNNNNNNGG	5 cut(s)	572, 742, 763, 989, 1612
BsmAI	GTCTC	1 cut(s)	389
BsmFI	GGGAC	1 cut(s)	1520
BsnI	GGCC	6 cut(s)	10, 393, 675, 851, 1340, 1447
Bso31I	GGTCTC	1 cut(s)	389
BsoBI	CYCGRG	1 cut(s)	1323
Bsp119I	TTCGAA	1 cut(s)	902
Bsp1286I	GDGCHC	2 cut(s)	355, 801
Bsp143I	GATC	6 cut(s)	40, 98, 562, 577, 693, 1577
Bsp1720I	GCTNAGC	1 cut(s)	188
BspANI	GGCC	6 cut(s)	10, 393, 675, 851, 1340, 1447
BspCNI	CTCAG	3 cut(s)	201, 1525, 1620
BspFNI	CGCG	3 cut(s)	156, 336, 1310
BspLI	GGNNCC	3 cut(s)	401, 564, 1579
BspMAI	CTGCAG	1 cut(s)	1401
BspMI	ACCTGC	1 cut(s)	493
BspPI	GGATC	5 cut(s)	93, 557, 570, 1572, 1585
BspQI	GCTCTTC	1 cut(s)	1656
BspT104I	TTCGAA	1 cut(s)	902
BspTNI	GGTCTC	1 cut(s)	389
BsrDI	GCAATG	3 cut(s)	725, 1224, 1674
BsrFI	RCCGGY	1 cut(s)	6
BsrI	ACTGG	4 cut(s)	632, 772, 1349, 1393
BssAI	RCCGGY	1 cut(s)	6
BssECI	CCNNGG	6 cut(s)	571, 852, 1210, 1308, 1323, 1654
BssMI	GATC	6 cut(s)	40, 98, 562, 577, 693, 1577
BssNI	GRCGYC	1 cut(s)	126
BssT1I	CCWWGG	1 cut(s)	1654
Bst2UI	CCWGG	2 cut(s)	449, 1211
Bst4CI	ACNGT	2 cut(s)	784, 1024
Bst6I	CTCTTC	7 cut(s)	28, 245, 260, 287, 362, 392, 1656
BstACI	GRCGYC	1 cut(s)	126
BstBI	TTCGAA	1 cut(s)	902
BstC8I	GCNNGC	5 cut(s)	74, 351, 984, 1305, 1449
BstDEI	CTNAG	3 cut(s)	188, 1512, 1607
BstDSI	CCRYGG	1 cut(s)	1308
BstENI	CCTNNNNNAGG	1 cut(s)	1610
BstF5I	GGATG	8 cut(s)	231, 249, 409, 870, 931, 1036, 1084, 1480
BstFNI	CGCG	3 cut(s)	156, 336, 1310
BstHHI	GCGC	2 cut(s)	72, 1605
BstKTI	GATC	6 cut(s)	43, 101, 565, 580, 696, 1580
BstMAI	GTCTC	1 cut(s)	389
BstMBI	GATC	6 cut(s)	40, 98, 562, 577, 693, 1577
BstMCI	CGRYCG	3 cut(s)	11, 43, 580
BstMWI	GCNNNNNNNGC	8 cut(s)	82, 153, 370, 582, 672, 1168, 1177, 1337
BstNI	CCWGG	2 cut(s)	449, 1211
BstNSI	RCATGY	1 cut(s)	715
BstSCI	CCNGG	7 cut(s)	447, 558, 735, 1120, 1209, 1322, 1323
BstSFI	CTRYAG	1 cut(s)	1397
BstUI	CGCG	3 cut(s)	156, 336, 1310
BstV1I	GCAGC	7 cut(s)	94, 385, 388, 734, 1129, 1288, 1385
BstV2I	GAAGAC	1 cut(s)	1025
BstX2I	RGATCY	2 cut(s)	562, 1577
BstYI	RGATCY	2 cut(s)	562, 1577
BstZI	CGGCCG	1 cut(s)	8
Bsu36I	CCTNAGG	1 cut(s)	1607
BsuRI	GGCC	6 cut(s)	10, 393, 675, 851, 1340, 1447
BtgI	CCRYGG	1 cut(s)	1308
BtsCI	GGATG	8 cut(s)	231, 249, 409, 870, 931, 1036, 1084, 1480
BtsIMutI	CAGTG	1 cut(s)	780
BveI	ACCTGC	1 cut(s)	493
Cac8I	GCNNGC	5 cut(s)	74, 351, 984, 1305, 1449
CaiI	CAGNNNCTG	1 cut(s)	1631
CfoI	GCGC	2 cut(s)	72, 1605
Cfr10I	RCCGGY	1 cut(s)	6
Cfr13I	GGNCC	5 cut(s)	4, 595, 673, 1127, 1338
Cfr42I	CCGCGG	1 cut(s)	1311
Cfr9I	CCCGGG	1 cut(s)	1323
CseI	GACGC	1 cut(s)	919
Csp6I	GTAC	4 cut(s)	649, 775, 1430, 1645
CviAII	CATG	4 cut(s)	712, 1247, 1493, 1549
CviQI	GTAC	4 cut(s)	649, 775, 1430, 1645
DdeI	CTNAG	3 cut(s)	188, 1512, 1607
DpnI	GATC	6 cut(s)	42, 100, 564, 579, 695, 1579
DpnII	GATC	6 cut(s)	40, 98, 562, 577, 693, 1577
DrdI	GACNNNNNNGTC	1 cut(s)	131
DseDI	GACNNNNNNGTC	1 cut(s)	131
EaeI	YGGCCR	2 cut(s)	8, 849
EagI	CGGCCG	1 cut(s)	8
Eam1104I	CTCTTC	7 cut(s)	28, 245, 260, 287, 362, 392, 1656
EarI	CTCTTC	7 cut(s)	28, 245, 260, 287, 362, 392, 1656
EciI	GGCGGA	1 cut(s)	91
Ecl136II	GAGCTC	1 cut(s)	353
EclXI	CGGCCG	1 cut(s)	8
Eco130I	CCWWGG	1 cut(s)	1654
Eco24I	GRGCYC	1 cut(s)	355
Eco31I	GGTCTC	1 cut(s)	389
Eco47I	GGWCC	3 cut(s)	4, 595, 1127
Eco52I	CGGCCG	1 cut(s)	8
Eco53kI	GAGCTC	1 cut(s)	353
Eco57I	CTGAAG	1 cut(s)	968
Eco81I	CCTNAGG	1 cut(s)	1607
Eco88I	CYCGRG	1 cut(s)	1323
EcoICRI	GAGCTC	1 cut(s)	353
EcoNI	CCTNNNNNAGG	1 cut(s)	1610
EcoRII	CCWGG	2 cut(s)	447, 1209
EcoT14I	CCWWGG	1 cut(s)	1654
EcoT22I	ATGCAT	1 cut(s)	815
EcoT38I	GRGCYC	1 cut(s)	355
ErhI	CCWWGG	1 cut(s)	1654
FaeI	CATG	4 cut(s)	715, 1250, 1496, 1552
FalI	AAGNNNNNCTT	2 cut(s)	266, 298
FaqI	GGGAC	1 cut(s)	1520
FatI	CATG	4 cut(s)	711, 1246, 1492, 1548
FblI	GTMKAC	1 cut(s)	135
Fnu4HI	GCNGC	9 cut(s)	83, 374, 377, 723, 1118, 1244, 1277, 1308, 1374
FokI	GGATG	8 cut(s)	218, 236, 396, 857, 938, 1043, 1091, 1487
FriOI	GRGCYC	1 cut(s)	355
Fsp4HI	GCNGC	9 cut(s)	83, 374, 377, 723, 1118, 1244, 1277, 1308, 1374
FspBI	CTAG	3 cut(s)	753, 1133, 1406
GlaI	GCGC	2 cut(s)	71, 1604
GluI	GCNGC	9 cut(s)	83, 374, 377, 723, 1118, 1244, 1277, 1308, 1374
GsaI	CCCAGC	2 cut(s)	93, 1192
GsuI	CTGGAG	1 cut(s)	431
HaeIII	GGCC	6 cut(s)	10, 393, 675, 851, 1340, 1447
HapII	CCGG	7 cut(s)	7, 11, 559, 736, 1121, 1240, 1324
HgaI	GACGC	1 cut(s)	919
HhaI	GCGC	2 cut(s)	72, 1605
Hin1I	GRCGYC	1 cut(s)	126
Hin1II	CATG	4 cut(s)	715, 1250, 1496, 1552
Hin6I	GCGC	2 cut(s)	70, 1603
HinP1I	GCGC	2 cut(s)	70, 1603
HincII	GTYRAC	2 cut(s)	130, 136
HindII	GTYRAC	2 cut(s)	130, 136
HpaII	CCGG	7 cut(s)	7, 11, 559, 736, 1121, 1240, 1324
HphI	GGTGA	6 cut(s)	253, 757, 1067, 1123, 1312, 1670
Hpy166II	GTNNAC	4 cut(s)	130, 136, 595, 1394
Hpy188III	TCNNGA	8 cut(s)	274, 397, 940, 970, 977, 1255, 1575, 1609
Hpy8I	GTNNAC	4 cut(s)	130, 136, 595, 1394
Hpy99I	CGWCG	9 cut(s)	32, 35, 38, 41, 125, 128, 137, 168, 913
HpyAV	CCTTC	3 cut(s)	943, 1417, 1606
HpyCH4III	ACNGT	2 cut(s)	784, 1024
HpyCH4IV	ACGT	3 cut(s)	126, 132, 1205
HpyF10VI	GCNNNNNNNGC	8 cut(s)	82, 153, 370, 582, 672, 1168, 1177, 1337
HpyF3I	CTNAG	3 cut(s)	188, 1512, 1607
HpySE526I	ACGT	3 cut(s)	126, 132, 1205
Hsp92I	GRCGYC	1 cut(s)	126
Hsp92II	CATG	4 cut(s)	715, 1250, 1496, 1552
HspAI	GCGC	2 cut(s)	70, 1603
KspI	CCGCGG	1 cut(s)	1311
Kzo9I	GATC	6 cut(s)	40, 98, 562, 577, 693, 1577
LguI	GCTCTTC	1 cut(s)	1656
LmnI	GCTCC	4 cut(s)	90, 336, 369, 1568
Lsp1109I	GCAGC	7 cut(s)	94, 385, 388, 734, 1129, 1288, 1385
LweI	GCATC	8 cut(s)	85, 418, 943, 1104, 1326, 1360, 1465, 1580
MaeI	CTAG	3 cut(s)	753, 1133, 1406
MaeII	ACGT	3 cut(s)	126, 132, 1205
MaeIII	GTNAC	2 cut(s)	832, 1401
MalI	GATC	6 cut(s)	42, 100, 564, 579, 695, 1579
MboI	GATC	6 cut(s)	40, 98, 562, 577, 693, 1577
MfeI	CAATTG	1 cut(s)	621
MflI	RGATCY	2 cut(s)	562, 1577
MhlI	GDGCHC	2 cut(s)	355, 801
MluCI	AATT	9 cut(s)	415, 436, 621, 637, 845, 960, 1219, 1456, 1497
MlyI	GAGTC	5 cut(s)	8, 347, 982, 1144, 1502
MmeI	TCCRAC	3 cut(s)	51, 708, 768
Mph1103I	ATGCAT	1 cut(s)	815
MroXI	GAANNNNTTC	1 cut(s)	1220
MseI	TTAA	2 cut(s)	804, 1440
MspA1I	CMGCKG	4 cut(s)	601, 1310, 1376, 1463
MspI	CCGG	7 cut(s)	7, 11, 559, 736, 1121, 1240, 1324
MspR9I	CCNGG	7 cut(s)	449, 560, 737, 1122, 1211, 1324, 1325
MunI	CAATTG	1 cut(s)	621
MvaI	CCWGG	2 cut(s)	449, 1211
MvnI	CGCG	3 cut(s)	156, 336, 1310
MwoI	GCNNNNNNNGC	8 cut(s)	82, 153, 370, 582, 672, 1168, 1177, 1337
NciI	CCSGG	5 cut(s)	560, 737, 1122, 1324, 1325
NdeII	GATC	6 cut(s)	40, 98, 562, 577, 693, 1577
NlaIII	CATG	4 cut(s)	715, 1250, 1496, 1552
NlaIV	GGNNCC	3 cut(s)	401, 564, 1579
NmeAIII	GCCGAG	3 cut(s)	552, 877, 1035
NmuCI	GTSAC	1 cut(s)	1401
NsiI	ATGCAT	1 cut(s)	815
NspI	RCATGY	1 cut(s)	715
NspV	TTCGAA	1 cut(s)	902
PaqCI	CACCTGC	1 cut(s)	493
PciI	ACATGT	1 cut(s)	711
PciSI	GCTCTTC	1 cut(s)	1656
PcsI	WCGNNNNNNNCGW	5 cut(s)	36, 63, 129, 132, 1450
PdmI	GAANNNNTTC	1 cut(s)	1220
PfeI	GAWTC	5 cut(s)	314, 496, 545, 748, 865
PflFI	GACNNNGTC	1 cut(s)	125
PfoI	TCCNGGA	2 cut(s)	447, 558
PkrI	GCNGC	9 cut(s)	84, 375, 378, 724, 1119, 1245, 1278, 1309, 1375
Ple19I	CGATCG	2 cut(s)	43, 580
PleI	GAGTC	5 cut(s)	8, 346, 981, 1144, 1502
PpsI	GAGTC	5 cut(s)	8, 346, 981, 1144, 1502
PscI	ACATGT	1 cut(s)	711
PsiI	TTATAA	1 cut(s)	1175
Psp124BI	GAGCTC	1 cut(s)	355
Psp6I	CCWGG	2 cut(s)	447, 1209
PspFI	CCCAGC	2 cut(s)	89, 1188
PspGI	CCWGG	2 cut(s)	447, 1209
PspN4I	GGNNCC	3 cut(s)	401, 564, 1579
PspPI	GGNCC	5 cut(s)	4, 595, 673, 1127, 1338
PstI	CTGCAG	1 cut(s)	1401
PstNI	CAGNNNCTG	1 cut(s)	1631
PsuI	RGATCY	2 cut(s)	562, 1577
PsyI	GACNNNGTC	1 cut(s)	125
PvuI	CGATCG	2 cut(s)	43, 580
PvuII	CAGCTG	2 cut(s)	1376, 1463
RsaI	GTAC	4 cut(s)	650, 776, 1431, 1646
RsaNI	GTAC	4 cut(s)	649, 775, 1430, 1645
SacI	GAGCTC	1 cut(s)	355
SacII	CCGCGG	1 cut(s)	1311
SalI	GTCGAC	1 cut(s)	134
SapI	GCTCTTC	1 cut(s)	1656
SaqAI	TTAA	2 cut(s)	804, 1440
SatI	GCNGC	9 cut(s)	83, 374, 377, 723, 1118, 1244, 1277, 1308, 1374
Sau3AI	GATC	6 cut(s)	40, 98, 562, 577, 693, 1577
Sau96I	GGNCC	5 cut(s)	4, 595, 673, 1127, 1338
ScaI	AGTACT	1 cut(s)	650
SchI	GAGTC	5 cut(s)	8, 347, 982, 1144, 1502
ScrFI	CCNGG	7 cut(s)	449, 560, 737, 1122, 1211, 1324, 1325
SduI	GDGCHC	2 cut(s)	355, 801
SfaNI	GCATC	8 cut(s)	85, 418, 943, 1104, 1326, 1360, 1465, 1580
SfcI	CTRYAG	1 cut(s)	1397
Sfr303I	CCGCGG	1 cut(s)	1311
SfuI	TTCGAA	1 cut(s)	902
SgrBI	CCGCGG	1 cut(s)	1311
SinI	GGWCC	3 cut(s)	4, 595, 1127
SmaI	CCCGGG	1 cut(s)	1325
SmlI	CTYRAG	2 cut(s)	182, 938
SmoI	CTYRAG	2 cut(s)	182, 938
SpeI	ACTAGT	1 cut(s)	1405
Sse9I	AATT	9 cut(s)	415, 436, 621, 637, 845, 960, 1219, 1456, 1497
SspMI	CTAG	3 cut(s)	753, 1133, 1406
SstI	GAGCTC	1 cut(s)	355
StyD4I	CCNGG	7 cut(s)	447, 558, 735, 1120, 1209, 1322, 1323
StyI	CCWWGG	1 cut(s)	1654
TaaI	ACNGT	2 cut(s)	784, 1024
TaiI	ACGT	3 cut(s)	129, 135, 1208
TaqI	TCGA	8 cut(s)	57, 97, 120, 135, 464, 840, 902, 1049
TaqII	GACCGA	1 cut(s)	371
TasI	AATT	9 cut(s)	415, 436, 621, 637, 845, 960, 1219, 1456, 1497
TatI	WGTACW	2 cut(s)	648, 774
TauI	GCSGC	2 cut(s)	1246, 1310
TfiI	GAWTC	5 cut(s)	314, 496, 545, 748, 865
Tru1I	TTAA	2 cut(s)	804, 1440
Tru9I	TTAA	2 cut(s)	804, 1440
TscAI	CASTG	1 cut(s)	787
TseFI	GTSAC	1 cut(s)	1401
TseI	GCWGC	7 cut(s)	82, 373, 376, 722, 1117, 1276, 1373
Tsp45I	GTSAC	1 cut(s)	1401
TspDTI	ATGAA	6 cut(s)	214, 220, 1186, 1213, 1552, 1663
TspGWI	ACGGA	1 cut(s)	538
TspMI	CCCGGG	1 cut(s)	1323
TspRI	CASTG	1 cut(s)	787
Tth111I	GACNNNGTC	1 cut(s)	125
VpaK11BI	GGWCC	3 cut(s)	4, 595, 1127
XagI	CCTNNNNNAGG	1 cut(s)	1610
XapI	RAATTY	3 cut(s)	960, 1219, 1456
XceI	RCATGY	1 cut(s)	715
XmaI	CCCGGG	1 cut(s)	1323
XmiI	GTMKAC	1 cut(s)	135
XmnI	GAANNNNTTC	1 cut(s)	1220
XspI	CTAG	3 cut(s)	753, 1133, 1406
ZraI	GACGTC	1 cut(s)	127
ZrmI	AGTACT	1 cut(s)	650
Zsp2I	ATGCAT	1 cut(s)	815

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.