Rh2DG609000

Belongs to the tRNA nucleotidyltransferase poly(A) polymerase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0020719
Physical Location & Seq
Forward (+)
84229011 .. 84229970
960 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG609000.1

Sequence Viewer

Length: 426 bp
ATGAACACAGAGCTCAATGAAACCGAGACCAAGATCTTCGATATCCTTCTCAAAACACTATGCCATAATGGCCTTGAGAACCTGCAGCTTCATGTTGCTGATGGATGGGTCCGTGATAAGCTTCGGGGCAAAGACTATGGGGACATTGATATTGCAGTTGAAGACATGAGGGGAATCAAATTTGCTGAGAAGGTGAAAGACTACTTGTACTTTGTTGGGAAGGAGCAAGCAATCACTTATTTTCCAGGCAAATTACAACGTGTGGCAGCGGCAAGGATGAGCATGTTTAATATATGGCTTGATTTTTTTAAGCTAGGGGGTGAAAATAACAGTGACAATAGCCCCATTAGTTCCACTGATGAGCAAAAATATGCCACAGAAAAAGAGAAGGATGCATGTAGAAGAGATATAACCATTAACAGTTGA

Protein Analysis

141

Amino Acids

16.22

Weight (kDa)

5.24

Isoelectric Point (pI)

38.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PolyA_pol PF01743 31 - 141 1.4e-09 Poly A polymerase head domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0020719)

Species Orthologous Gene IDs
rosa_multiflora Rmu_sc0000124.1_g000040
rosa_rugosa Rorug02G0521400.1
rosa_samantha Rh2DG609000 Rh4BG167400 Rh4BG393300 Rh4CG408100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 90
AciI CCGC 1 cut(s) 269
AcsI RAATTY 1 cut(s) 179
AfaI GTAC 1 cut(s) 209
AflIII ACRYGT 1 cut(s) 259
AgsI TTSAA 1 cut(s) 161
AjnI CCWGG 1 cut(s) 244
AluBI AGCT 4 cut(s) 13, 88, 121, 313
AluI AGCT 4 cut(s) 13, 88, 121, 313
Alw21I GWGCWC 1 cut(s) 15
Alw26I GTCTC 1 cut(s) 20
AoxI GGCC 1 cut(s) 70
ApeKI GCWGC 2 cut(s) 85, 266
ApoI RAATTY 1 cut(s) 179
AspS9I GGNCC 1 cut(s) 109
AsuHPI GGTGA 2 cut(s) 205, 332
AvaII GGWCC 1 cut(s) 109
BanII GRGCYC 1 cut(s) 15
BbsI GAAGAC 1 cut(s) 168
Bbv12I GWGCWC 1 cut(s) 15
BbvI GCAGC 2 cut(s) 97, 278
BccI CCATC 2 cut(s) 95, 99
BciT130I CCWGG 1 cut(s) 246
BcoDI GTCTC 1 cut(s) 20
BfaI CTAG 1 cut(s) 314
BfmI CTRYAG 1 cut(s) 83
BfuAI ACCTGC 1 cut(s) 90
BglI GCCNNNNNGGC 1 cut(s) 69
BglII AGATCT 1 cut(s) 33
BisI GCNGC 3 cut(s) 86, 267, 270
BlsI GCNGC 3 cut(s) 87, 268, 271
Bme1390I CCNGG 1 cut(s) 246
Bme18I GGWCC 1 cut(s) 109
BmgT120I GGNCC 1 cut(s) 109
BmiI GGNNCC 1 cut(s) 110
BmrFI CCNGG 1 cut(s) 246
BmsI GCATC 1 cut(s) 382
BpiI GAAGAC 1 cut(s) 168
BpuEI CTTGAG 1 cut(s) 95
BsaI GGTCTC 1 cut(s) 20
BseBI CCWGG 1 cut(s) 246
BseGI GGATG 3 cut(s) 110, 282, 397
BseMII CTCAG 1 cut(s) 177
BseXI GCAGC 2 cut(s) 97, 278
BshFI GGCC 1 cut(s) 72
BsiHKAI GWGCWC 1 cut(s) 15
BslFI GGGAC 1 cut(s) 155
BsmAI GTCTC 1 cut(s) 20
BsmFI GGGAC 1 cut(s) 155
BsnI GGCC 1 cut(s) 72
Bso31I GGTCTC 1 cut(s) 20
Bsp1286I GDGCHC 1 cut(s) 15
Bsp143I GATC 1 cut(s) 33
BspACI CCGC 1 cut(s) 269
BspANI GGCC 1 cut(s) 72
BspCNI CTCAG 1 cut(s) 178
BspLI GGNNCC 1 cut(s) 110
BspMAI CTGCAG 1 cut(s) 87
BspMI ACCTGC 1 cut(s) 90
BspTNI GGTCTC 1 cut(s) 20
BssMI GATC 1 cut(s) 33
Bst2UI CCWGG 1 cut(s) 246
Bst4CI ACNGT 2 cut(s) 332, 422
Bst6I CTCTTC 1 cut(s) 397
BstC8I GCNNGC 1 cut(s) 228
BstDEI CTNAG 1 cut(s) 186
BstF5I GGATG 3 cut(s) 110, 282, 397
BstKTI GATC 1 cut(s) 36
BstMAI GTCTC 1 cut(s) 20
BstMBI GATC 1 cut(s) 33
BstMWI GCNNNNNNNGC 1 cut(s) 69
BstNI CCWGG 1 cut(s) 246
BstNSI RCATGY 2 cut(s) 286, 399
BstSCI CCNGG 1 cut(s) 244
BstSFI CTRYAG 1 cut(s) 83
BstV1I GCAGC 2 cut(s) 97, 278
BstV2I GAAGAC 1 cut(s) 168
BstX2I RGATCY 1 cut(s) 33
BstYI RGATCY 1 cut(s) 33
BsuRI GGCC 1 cut(s) 72
BtsCI GGATG 3 cut(s) 110, 282, 397
BtsIMutI CAGTG 2 cut(s) 337, 354
BveI ACCTGC 1 cut(s) 90
Cac8I GCNNGC 1 cut(s) 228
Cfr13I GGNCC 1 cut(s) 109
Csp6I GTAC 1 cut(s) 208
CviAII CATG 4 cut(s) 92, 166, 283, 396
CviJI RGCY 7 cut(s) 13, 72, 88, 121, 298, 313, 342
CviKI_1 RGCY 7 cut(s) 13, 72, 88, 121, 298, 313, 342
CviQI GTAC 1 cut(s) 208
DdeI CTNAG 1 cut(s) 186
DpnI GATC 1 cut(s) 35
DpnII GATC 1 cut(s) 33
Eam1104I CTCTTC 1 cut(s) 397
EarI CTCTTC 1 cut(s) 397
Ecl136II GAGCTC 1 cut(s) 13
Eco24I GRGCYC 1 cut(s) 15
Eco31I GGTCTC 1 cut(s) 20
Eco32I GATATC 1 cut(s) 43
Eco47I GGWCC 1 cut(s) 109
Eco53kI GAGCTC 1 cut(s) 13
EcoICRI GAGCTC 1 cut(s) 13
EcoRII CCWGG 1 cut(s) 244
EcoRV GATATC 1 cut(s) 43
EcoT22I ATGCAT 1 cut(s) 397
EcoT38I GRGCYC 1 cut(s) 15
FaeI CATG 4 cut(s) 95, 169, 286, 399
FaqI GGGAC 1 cut(s) 155
FatI CATG 4 cut(s) 91, 165, 282, 395
Fnu4HI GCNGC 3 cut(s) 86, 267, 270
FokI GGATG 3 cut(s) 117, 289, 404
FriOI GRGCYC 1 cut(s) 15
Fsp4HI GCNGC 3 cut(s) 86, 267, 270
FspBI CTAG 1 cut(s) 314
GluI GCNGC 3 cut(s) 86, 267, 270
HaeIII GGCC 1 cut(s) 72
Hin1II CATG 4 cut(s) 95, 169, 286, 399
HindIII AAGCTT 1 cut(s) 119
HinfI GANTC 1 cut(s) 174
HphI GGTGA 2 cut(s) 205, 332
HpyAV CCTTC 4 cut(s) 56, 184, 214, 382
HpyCH4III ACNGT 2 cut(s) 332, 422
HpyCH4IV ACGT 1 cut(s) 259
HpyCH4V TGCA 3 cut(s) 85, 155, 395
HpyF10VI GCNNNNNNNGC 1 cut(s) 69
HpyF3I CTNAG 1 cut(s) 186
HpySE526I ACGT 1 cut(s) 259
Hsp92II CATG 4 cut(s) 95, 169, 286, 399
Kzo9I GATC 1 cut(s) 33
LmnI GCTCC 1 cut(s) 223
LpnPI CCDG 3 cut(s) 95, 231, 258
Lsp1109I GCAGC 2 cut(s) 97, 278
LweI GCATC 1 cut(s) 382
MaeI CTAG 1 cut(s) 314
MaeII ACGT 1 cut(s) 259
MaeIII GTNAC 1 cut(s) 332
MalI GATC 1 cut(s) 35
MboI GATC 1 cut(s) 33
MboII GAAGA 3 cut(s) 28, 173, 414
MflI RGATCY 1 cut(s) 33
MhlI GDGCHC 1 cut(s) 15
MluCI AATT 2 cut(s) 179, 251
MnlI CCTC 1 cut(s) 162
Mph1103I ATGCAT 1 cut(s) 397
MseI TTAA 3 cut(s) 288, 309, 417
MspA1I CMGCKG 1 cut(s) 269
MspR9I CCNGG 1 cut(s) 246
MvaI CCWGG 1 cut(s) 246
MwoI GCNNNNNNNGC 1 cut(s) 69
NdeII GATC 1 cut(s) 33
NlaIII CATG 4 cut(s) 95, 169, 286, 399
NlaIV GGNNCC 1 cut(s) 110
NmuCI GTSAC 1 cut(s) 332
NsiI ATGCAT 1 cut(s) 397
NspI RCATGY 2 cut(s) 286, 399
PfeI GAWTC 1 cut(s) 174
PkrI GCNGC 3 cut(s) 87, 268, 271
Psp124BI GAGCTC 1 cut(s) 15
Psp6I CCWGG 1 cut(s) 244
PspGI CCWGG 1 cut(s) 244
PspN4I GGNNCC 1 cut(s) 110
PspPI GGNCC 1 cut(s) 109
PstI CTGCAG 1 cut(s) 87
PsuI RGATCY 1 cut(s) 33
RsaI GTAC 1 cut(s) 209
RsaNI GTAC 1 cut(s) 208
SacI GAGCTC 1 cut(s) 15
SaqAI TTAA 3 cut(s) 288, 309, 417
SatI GCNGC 3 cut(s) 86, 267, 270
Sau3AI GATC 1 cut(s) 33
Sau96I GGNCC 1 cut(s) 109
ScrFI CCNGG 1 cut(s) 246
SduI GDGCHC 1 cut(s) 15
SetI ASST 7 cut(s) 15, 84, 90, 123, 195, 262, 315
SfaNI GCATC 1 cut(s) 382
SfcI CTRYAG 1 cut(s) 83
SinI GGWCC 1 cut(s) 109
SmlI CTYRAG 1 cut(s) 74
SmoI CTYRAG 1 cut(s) 74
Sse9I AATT 2 cut(s) 179, 251
SsiI CCGC 1 cut(s) 269
SspMI CTAG 1 cut(s) 314
SstI GAGCTC 1 cut(s) 15
StyD4I CCNGG 1 cut(s) 244
TaaI ACNGT 2 cut(s) 332, 422
TaiI ACGT 1 cut(s) 262
TaqI TCGA 1 cut(s) 39
TasI AATT 2 cut(s) 179, 251
TatI WGTACW 1 cut(s) 207
TauI GCSGC 1 cut(s) 272
TfiI GAWTC 1 cut(s) 174
Tru1I TTAA 3 cut(s) 288, 309, 417
Tru9I TTAA 3 cut(s) 288, 309, 417
TscAI CASTG 2 cut(s) 337, 361
TseFI GTSAC 1 cut(s) 332
TseI GCWGC 2 cut(s) 85, 266
Tsp45I GTSAC 1 cut(s) 332
TspDTI ATGAA 3 cut(s) 17, 33, 80
TspGWI ACGGA 1 cut(s) 101
TspRI CASTG 2 cut(s) 337, 361
VpaK11BI GGWCC 1 cut(s) 109
XapI RAATTY 1 cut(s) 179
XceI RCATGY 2 cut(s) 286, 399
XspI CTAG 1 cut(s) 314
Zsp2I ATGCAT 1 cut(s) 397
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.