Rh3AG080600

Cyclic phosphodiesterase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3A
OG0019912
Physical Location & Seq
Forward (+)
6449939 .. 6457519
7581 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3AG080600.1

Sequence Viewer

Length: 1005 bp
ATGTGGAAGGATCTGACAGGGATGGTGACTCTGAGTAACGGTGAGATTCGGACGGCTTTGGAGGGTTTAACTGGTTATGCAGAGCCTGAAAGCTTCAAAGCTTTGATGGGCCCTTCTGGCTCTGGCAAATCGACCTTGCTTGATGCTCTCTCTAGCCGTTTGGCTTCGAATGCCTTCCATCTGGCTCTGTTTTACTCAATGGATGCAAAAGAAAGCTCTCTTTGGGCACAGCTTGCTTCAGCATTTTTTATAACACAGACACTACGTGCCCTATCACGAGATGGAAGGACTGTGATAGCTTCAATTCACAAGCCAAACAGTGAAGTCTTTGAACTATTTGATCGACTGTACTTGCTTTCTGGTGGCAACACAGTTTACTTTGGTCAGGCCTCTGAGGCATATGAGGCAGATTTTTCTATGTACAAGTACTCCCCATTTAGTTATTCGATGGTAGACAAGGAATGTTACTCGTATTCAGTGTGGGCCATCCCATCAGATGATGTATCTTTCAGGATCATGAAGTTAATGGAGAGCCTTCGGACCGAATTTGTTGGGCCGGAGATCAAGCCCCACATCCCTGTTGTAGGGTCCATTTGTATGACGCGTGATGAGGTGCTGAGCAAGTTTACATCTCTCCATTCTCATGTTGTTTATTCGTACAAAGCTAAAGTTGATCAGGTGGTTACTAGAAATTTTTATTACCAGTGTGTTTCCCTTCTCATTGATTCATCCTTTGAAGTTTCCAGCGAGTTACTGGAGGCAACTAAACAGTGCAGTGCACACTTCGGTTTCTATAAAGGTCCTATGCCGCGTTTGAGCCTCTTTTGCGGGAATCTGACAAGTGGAGAGAGGAAATTAGCTCAAGAGAAAGTCAATGTTATGGACAGAAGCATTACTAGCCTGAGCTTTCCCATAACTCGACTTGCATTGTACAAAGTTGATTACAAAGATACAACTCTCAATTCATGGGAGAAGATTGCGGAATATCCCCTCTTATTGAATTAG

Protein Analysis

334

Amino Acids

37.55

Weight (kDa)

6.11

Isoelectric Point (pI)

27.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AAA_29 PF13555 33 - 57 3.5e-06 P-loop containing region of AAA domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0019912)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr3g0457431
rosa_laevigata RLG00000025230
rosa_multiflora Rmu_co8009242.1_g000001
rosa_samantha Rh3AG080600 Rh3BG082500 Rh3CG082700 Rh3DG084200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 251
AccI GTMKAC 1 cut(s) 453
AccII CGCG 2 cut(s) 604, 811
AciI CCGC 3 cut(s) 809, 828, 980
AclWI GGATC 2 cut(s) 18, 521
AcsI RAATTY 2 cut(s) 545, 691
AcuI CTGAAG 1 cut(s) 222
AdeI CACNNNGTG 1 cut(s) 266
AfaI GTAC 5 cut(s) 350, 422, 428, 659, 932
AfiI CCNNNNNNNGG 1 cut(s) 584
AflIII ACRYGT 1 cut(s) 602
AgsI TTSAA 5 cut(s) 97, 303, 332, 737, 1000
AluBI AGCT 8 cut(s) 93, 101, 216, 232, 299, 665, 860, 906
AluI AGCT 8 cut(s) 93, 101, 216, 232, 299, 665, 860, 906
Alw21I GWGCWC 1 cut(s) 781
Alw44I GTGCAC 1 cut(s) 777
AlwI GGATC 2 cut(s) 18, 521
AlwNI CAGNNNCTG 1 cut(s) 86
AoxI GGCC 4 cut(s) 109, 387, 483, 554
ApaI GGGCCC 1 cut(s) 113
ApaLI GTGCAC 1 cut(s) 777
ApoI RAATTY 2 cut(s) 545, 691
Asp700I GAANNNNTTC 1 cut(s) 173
AspS9I GGNCC 7 cut(s) 109, 110, 483, 540, 554, 588, 800
AsuHPI GGTGA 2 cut(s) 37, 53
AsuII TTCGAA 1 cut(s) 167
AvaII GGWCC 3 cut(s) 540, 588, 800
BaeGI GKGCMC 4 cut(s) 113, 229, 271, 781
BanII GRGCYC 1 cut(s) 113
BauI CACGAG 1 cut(s) 276
Bbv12I GWGCWC 1 cut(s) 781
BccI CCATC 7 cut(s) 16, 100, 186, 275, 442, 494, 499
BceAI ACGGC 2 cut(s) 69, 141
BclI TGATCA 1 cut(s) 673
BfaI CTAG 3 cut(s) 153, 687, 897
BglI GCCNNNNNGGC 2 cut(s) 117, 395
BisI GCNGC 1 cut(s) 809
BlpI GCTNAGC 1 cut(s) 617
BlsI GCNGC 1 cut(s) 810
BmcAI AGTACT 1 cut(s) 428
Bme18I GGWCC 3 cut(s) 540, 588, 800
BmgT120I GGNCC 7 cut(s) 109, 110, 483, 540, 554, 588, 800
BmiI GGNNCC 2 cut(s) 111, 589
BmsI GCATC 2 cut(s) 133, 193
BpmI CTGGAG 1 cut(s) 776
Bpu10I CCTNAGC 1 cut(s) 902
Bpu1102I GCTNAGC 1 cut(s) 617
Bpu14I TTCGAA 1 cut(s) 167
BpuEI CTTGAG 1 cut(s) 846
BsaAI YACGTR 1 cut(s) 266
BsaXI ACNNNNNCTCC 2 cut(s) 413, 443
Bsc4I CCNNNNNNNGG 1 cut(s) 584
Bse1I ACTGG 3 cut(s) 76, 703, 759
BseGI GGATG 5 cut(s) 27, 208, 486, 573, 728
BseLI CCNNNNNNNGG 1 cut(s) 584
BseMII CTCAG 4 cut(s) 23, 384, 608, 893
BseNI ACTGG 3 cut(s) 76, 703, 759
BseSI GKGCMC 4 cut(s) 113, 229, 271, 781
BsgI GTGCAG 1 cut(s) 793
Bsh1236I CGCG 2 cut(s) 604, 811
BshFI GGCC 4 cut(s) 111, 389, 485, 556
BsiHKAI GWGCWC 1 cut(s) 781
BsiSI CCGG 1 cut(s) 557
BslI CCNNNNNNNGG 1 cut(s) 584
BsmI GAATGC 1 cut(s) 175
BsnI GGCC 4 cut(s) 111, 389, 485, 556
Bsp119I TTCGAA 1 cut(s) 167
Bsp120I GGGCCC 1 cut(s) 109
Bsp1286I GDGCHC 4 cut(s) 113, 229, 271, 781
Bsp1407I TGTACA 2 cut(s) 420, 930
Bsp143I GATC 5 cut(s) 10, 340, 513, 561, 673
Bsp1720I GCTNAGC 1 cut(s) 617
BspACI CCGC 3 cut(s) 809, 828, 980
BspANI GGCC 4 cut(s) 111, 389, 485, 556
BspCNI CTCAG 4 cut(s) 24, 385, 609, 894
BspFNI CGCG 2 cut(s) 604, 811
BspHI TCATGA 1 cut(s) 516
BspLI GGNNCC 2 cut(s) 111, 589
BspPI GGATC 2 cut(s) 18, 521
BspT104I TTCGAA 1 cut(s) 167
BsrGI TGTACA 2 cut(s) 420, 930
BsrI ACTGG 3 cut(s) 76, 703, 759
BssMI GATC 5 cut(s) 10, 340, 513, 561, 673
BssSI CACGAG 1 cut(s) 276
Bst2BI CACGAG 1 cut(s) 276
Bst4CI ACNGT 6 cut(s) 41, 292, 320, 348, 373, 771
BstAPI GCANNNNNTGC 1 cut(s) 233
BstAUI TGTACA 2 cut(s) 420, 930
BstBAI YACGTR 1 cut(s) 266
BstBI TTCGAA 1 cut(s) 167
BstC8I GCNNGC 1 cut(s) 234
BstDEI CTNAG 4 cut(s) 32, 393, 617, 902
BstENI CCTNNNNNAGG 1 cut(s) 582
BstF5I GGATG 5 cut(s) 27, 208, 486, 573, 728
BstFNI CGCG 2 cut(s) 604, 811
BstKTI GATC 5 cut(s) 13, 343, 516, 564, 676
BstMBI GATC 5 cut(s) 10, 340, 513, 561, 673
BstMWI GCNNNNNNNGC 7 cut(s) 117, 170, 233, 395, 404, 825, 897
BstSLI GKGCMC 4 cut(s) 113, 229, 271, 781
BstUI CGCG 2 cut(s) 604, 811
BstX2I RGATCY 1 cut(s) 10
BstYI RGATCY 1 cut(s) 10
BsuRI GGCC 4 cut(s) 111, 389, 485, 556
BtsCI GGATG 5 cut(s) 27, 208, 486, 573, 728
BtsI GCAGTG 1 cut(s) 781
BtsIMutI CAGTG 5 cut(s) 325, 483, 710, 776, 781
Cac8I GCNNGC 1 cut(s) 234
CaiI CAGNNNCTG 1 cut(s) 86
CciI TCATGA 1 cut(s) 516
Cfr13I GGNCC 7 cut(s) 109, 110, 483, 540, 554, 588, 800
CpoI CGGWCCG 1 cut(s) 540
CseI GACGC 1 cut(s) 610
Csp6I GTAC 5 cut(s) 349, 421, 427, 658, 931
CspI CGGWCCG 1 cut(s) 540
CviAII CATG 3 cut(s) 517, 644, 966
CviQI GTAC 5 cut(s) 349, 421, 427, 658, 931
DdeI CTNAG 4 cut(s) 32, 393, 617, 902
DpnI GATC 5 cut(s) 12, 342, 515, 563, 675
DpnII GATC 5 cut(s) 10, 340, 513, 561, 673
DraIII CACNNNGTG 1 cut(s) 266
Eco147I AGGCCT 1 cut(s) 389
Eco24I GRGCYC 1 cut(s) 113
Eco47I GGWCC 3 cut(s) 540, 588, 800
Eco57I CTGAAG 1 cut(s) 222
EcoNI CCTNNNNNAGG 1 cut(s) 582
EcoO109I RGGNCCY 2 cut(s) 110, 800
EcoT38I GRGCYC 1 cut(s) 113
FaeI CATG 3 cut(s) 520, 647, 969
FatI CATG 3 cut(s) 516, 643, 965
FauI CCCGC 1 cut(s) 821
FauNDI CATATG 1 cut(s) 400
FbaI TGATCA 1 cut(s) 673
FblI GTMKAC 1 cut(s) 453
Fnu4HI GCNGC 1 cut(s) 809
FokI GGATG 5 cut(s) 34, 215, 473, 560, 715
FriOI GRGCYC 1 cut(s) 113
Fsp4HI GCNGC 1 cut(s) 809
FspBI CTAG 3 cut(s) 153, 687, 897
GluI GCNGC 1 cut(s) 809
GsuI CTGGAG 1 cut(s) 776
HaeIII GGCC 4 cut(s) 111, 389, 485, 556
HapII CCGG 1 cut(s) 557
HgaI GACGC 1 cut(s) 610
Hin1II CATG 3 cut(s) 520, 647, 969
HindIII AAGCTT 2 cut(s) 91, 99
HinfI GANTC 4 cut(s) 28, 46, 725, 832
HpaII CCGG 1 cut(s) 557
HphI GGTGA 2 cut(s) 37, 53
Hpy166II GTNNAC 4 cut(s) 376, 454, 627, 779
Hpy188I TCNGA 7 cut(s) 15, 33, 51, 394, 496, 540, 837
Hpy188III TCNNGA 4 cut(s) 276, 511, 517, 863
Hpy8I GTNNAC 4 cut(s) 376, 454, 627, 779
HpyAV CCTTC 5 cut(s) 123, 184, 279, 545, 725
HpyCH4III ACNGT 6 cut(s) 41, 292, 320, 348, 373, 771
HpyCH4IV ACGT 1 cut(s) 265
HpyCH4V TGCA 5 cut(s) 80, 206, 774, 779, 926
HpyF10VI GCNNNNNNNGC 7 cut(s) 117, 170, 233, 395, 404, 825, 897
HpyF3I CTNAG 4 cut(s) 32, 393, 617, 902
HpySE526I ACGT 1 cut(s) 265
Hsp92II CATG 3 cut(s) 520, 647, 969
Ksp22I TGATCA 1 cut(s) 673
Kzo9I GATC 5 cut(s) 10, 340, 513, 561, 673
LweI GCATC 2 cut(s) 133, 193
MaeI CTAG 3 cut(s) 153, 687, 897
MaeII ACGT 1 cut(s) 265
MaeIII GTNAC 5 cut(s) 25, 35, 464, 682, 750
MalI GATC 5 cut(s) 12, 342, 515, 563, 675
MboI GATC 5 cut(s) 10, 340, 513, 561, 673
MboII GAAGA 1 cut(s) 985
MflI RGATCY 1 cut(s) 10
MhlI GDGCHC 4 cut(s) 113, 229, 271, 781
MluCI AATT 6 cut(s) 303, 545, 691, 854, 961, 1000
MluI ACGCGT 1 cut(s) 602
MlyI GAGTC 1 cut(s) 22
MnlI CCTC 9 cut(s) 55, 388, 397, 400, 604, 751, 830, 843, 1001
MroXI GAANNNNTTC 1 cut(s) 173
MseI TTAA 2 cut(s) 68, 524
MslI CAYNNNNRTG 2 cut(s) 596, 642
MspI CCGG 1 cut(s) 557
Mva1269I GAATGC 1 cut(s) 175
MvnI CGCG 2 cut(s) 604, 811
MwoI GCNNNNNNNGC 7 cut(s) 117, 170, 233, 395, 404, 825, 897
NdeI CATATG 1 cut(s) 400
NdeII GATC 5 cut(s) 10, 340, 513, 561, 673
NlaIII CATG 3 cut(s) 520, 647, 969
NlaIV GGNNCC 2 cut(s) 111, 589
NmuCI GTSAC 1 cut(s) 25
NspV TTCGAA 1 cut(s) 167
PagI TCATGA 1 cut(s) 516
PceI AGGCCT 1 cut(s) 389
PctI GAATGC 1 cut(s) 175
PdmI GAANNNNTTC 1 cut(s) 173
PfeI GAWTC 3 cut(s) 46, 725, 832
PkrI GCNGC 1 cut(s) 810
PleI GAGTC 1 cut(s) 22
PpsI GAGTC 1 cut(s) 22
Ppu21I YACGTR 1 cut(s) 266
PpuMI RGGWCCY 1 cut(s) 800
PsiI TTATAA 1 cut(s) 251
Psp5II RGGWCCY 1 cut(s) 800
PspN4I GGNNCC 2 cut(s) 111, 589
PspOMI GGGCCC 1 cut(s) 109
PspPI GGNCC 7 cut(s) 109, 110, 483, 540, 554, 588, 800
PspPPI RGGWCCY 1 cut(s) 800
PstNI CAGNNNCTG 1 cut(s) 86
PsuI RGATCY 1 cut(s) 10
RsaI GTAC 5 cut(s) 350, 422, 428, 659, 932
RsaNI GTAC 5 cut(s) 349, 421, 427, 658, 931
RseI CAYNNNNRTG 2 cut(s) 596, 642
Rsr2I CGGWCCG 1 cut(s) 540
RsrII CGGWCCG 1 cut(s) 540
SaqAI TTAA 2 cut(s) 68, 524
SatI GCNGC 1 cut(s) 809
Sau3AI GATC 5 cut(s) 10, 340, 513, 561, 673
Sau96I GGNCC 7 cut(s) 109, 110, 483, 540, 554, 588, 800
ScaI AGTACT 1 cut(s) 428
SchI GAGTC 1 cut(s) 22
SduI GDGCHC 4 cut(s) 113, 229, 271, 781
SfaNI GCATC 2 cut(s) 133, 193
SfuI TTCGAA 1 cut(s) 167
SinI GGWCC 3 cut(s) 540, 588, 800
SmiMI CAYNNNNRTG 2 cut(s) 596, 642
SmlI CTYRAG 1 cut(s) 861
SmoI CTYRAG 1 cut(s) 861
Sse9I AATT 6 cut(s) 303, 545, 691, 854, 961, 1000
SseBI AGGCCT 1 cut(s) 389
SsiI CCGC 3 cut(s) 809, 828, 980
SspMI CTAG 3 cut(s) 153, 687, 897
StuI AGGCCT 1 cut(s) 389
TaaI ACNGT 6 cut(s) 41, 292, 320, 348, 373, 771
TaiI ACGT 1 cut(s) 268
TaqI TCGA 5 cut(s) 131, 167, 343, 446, 919
TaqII GACCGA 1 cut(s) 557
TasI AATT 6 cut(s) 303, 545, 691, 854, 961, 1000
TatI WGTACW 4 cut(s) 348, 420, 426, 930
TauI GCSGC 1 cut(s) 811
TfiI GAWTC 3 cut(s) 46, 725, 832
Tru1I TTAA 2 cut(s) 68, 524
Tru9I TTAA 2 cut(s) 68, 524
TscAI CASTG 5 cut(s) 325, 483, 710, 776, 781
TseFI GTSAC 1 cut(s) 25
Tsp45I GTSAC 1 cut(s) 25
TspDTI ATGAA 3 cut(s) 533, 717, 954
TspRI CASTG 5 cut(s) 325, 483, 710, 776, 781
VneI GTGCAC 1 cut(s) 777
VpaK11BI GGWCC 3 cut(s) 540, 588, 800
XagI CCTNNNNNAGG 1 cut(s) 582
XapI RAATTY 2 cut(s) 545, 691
XcmI CCANNNNNNNNNTGG 1 cut(s) 751
XmiI GTMKAC 1 cut(s) 453
XmnI GAANNNNTTC 1 cut(s) 173
XspI CTAG 3 cut(s) 153, 687, 897
ZrmI AGTACT 1 cut(s) 428
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.