Rh3BG219200

Signal recognition particle 54 kDa protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3B
OG0012308
Physical Location & Seq
Reverse (-)
19743058 .. 19747663
4606 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3BG219200.1

Sequence Viewer

Length: 1476 bp
ATGGAGGCCGTACATTTCCCGACCGTTTCTTCCCGCCATTTCTCCGCGACGACCACGAACAGAACCTTCTCCGATTCAAAGAGGATTCCGACGGCCAAACTGGGCTCTTCGTGGACTGCTCCACGCTCTCCTACTCGGCTCTCTTCTAGAAACTTGTTCACTAGAGAGATATGGGGTTGGGTAAATGCCAAGAAAGTTACCACTGGTAGAAAAGAGTTGCGTGGAATTGTGAGAGCTGAAATGTTTGGTCAATTGACCAGTGGCCTCGAATCCGCTTGGAACAAGCTCAAAGGCGAAGAGGTTTTGACCAAGGAAAATATTGTGGAGCCAATGAGAGAAATCAGGAGGGCTCTTTTGGAAGCTGATGTAAGCCTTCCTGTTGTGAGAAGGTTTGTGCAAGCTGTCAGTGACCAGGCTGTTGGTGTTGGGGTTATTCGCGGAGTCAAACCGGATCAGCAATTGGTTAAGGTAAAAGTGCCTGTATATACAGCAGGGACTGACACCAAACCTTCACAAATAGCTAGGCAAGGTTTAGAGGAGGCCAAAAAGAAGAACATAGATGTAGTAATAATGGATACTGCTGGAAGGCTTCAGATAGACAAAGTACTGATGGATGAGTTGAAAGAAGTAAAGCGGGAATTGAACCCCACAGAAACTTTACTCGTTGTGGATGCAATGACTGGCCAGGAAGCTGCAGCCCTGGTCACGACATTCAATGTAGAGATTGGGATTACTGGCGCTATTTTAACAAAGCTGGATGGAGATTCTAGAGGTGGAGCAGCTTTGAGTGTTAAAGAGGTATCAGGAAAGCCAATCAAGCTTGTTGGCCGAGGAGAGCGGATGGAGGACCTTGAACCTTTTTACCCCGATCGTATGGCAGGACGCATATTGGGAATGGGAGACGTTCTCTCATTTGTCGAGAAGGCACAAGAAGTTATGCGCCAAGAAGATGCTGAAGAATTGCAAAAGAAGATTATGAGTACAAAGTTTGACTTCAATGATTTCCTAAAGCAAACTCGTGCTGTTGCCCGTATGGGTTCCATGACTCGGGTTCTTGGAATGATCCCTGGAATGGGGAAGGTTACTCCTGCACAAATTCGAGAGGCAGAAAAGAGTTTAGTGATAATGGAATCTATGATTGAAGCAATGACCCCTGAGGAAAGGGAGAAGCCAGAATTGTTGGCCGAATCTCCCATTAGGAGAAAAAGAATTGCTCAAGAGTCTGGGAAAACAGAGCAGCAGGTGAGCCAACTTGTTGCTCAACTGTTCCAAATGCGAGTTCGTATGAAGAATCTAATGGGTGCAATGGAAGGCGGTGGATCTATACCTGGACTGAATAATCTTGAGGAAGCCCTCAAAGTTGATCAGAAGGCTCCTGCTGGTACTGCAAGGCGTAAGCGACGATCTGAACCAAGGAGGCAGTTTGGTGACTCGGCATCGAGGCCCAGTCCTCGTGGTTTCGGGGCCAGGAACTGA

Protein Analysis

491

Amino Acids

54.41

Weight (kDa)

9.85

Isoelectric Point (pI)

44.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SRP54_N PF02881 85 - 156 1.2e-15 SRP54-type protein, helical bundle domain
SRP54 PF00448 156 - 298 5.3e-50 SRP54-type protein, GTPase domain
SRP_SPB PF02978 329 - 429 1.9e-24 Signal peptide binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012308)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G03940
fragaria_vesca FvH4_6g19110 FvH4_6g19110
malus_domestica MD12G1027100.v1.1 MD14G1027200.v1.1
prunus_persica Prupe.7G104500_v2.0.a1
pyrus_communis pycom12g02600 pycom14g02590
rosa_chinensis RchiOBHm_Chr3g0474211
rosa_laevigata RLG00000023941
rosa_multiflora Rmu_sc0004507.1_g000022 Rmu_sc0015051.1_g000004
rosa_roxburghii Rroxscaffold_6G00407430
rosa_rugosa Rorug03G0139500
rosa_samantha Rh3AG190000 Rh3BG219200 Rh3CG214600 Rh3DG214700
rosa_wichuraiana Rw3G017330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1231
Acc36I ACCTGC 1 cut(s) 1231
AccBSI CCGCTC 1 cut(s) 838
AccII CGCG 2 cut(s) 47, 438
AciI CCGC 7 cut(s) 34, 45, 273, 438, 634, 838, 1314
AclWI GGATC 3 cut(s) 459, 1057, 1327
AcoI YGGCCR 4 cut(s) 93, 682, 826, 1182
AcsI RAATTY 1 cut(s) 1095
AcuI CTGAAG 2 cut(s) 575, 975
AfaI GTAC 4 cut(s) 12, 606, 982, 1384
AfiI CCNNNNNNNGG 3 cut(s) 1047, 1072, 1073
AgsI TTSAA 7 cut(s) 78, 622, 643, 715, 854, 997, 1142
AjnI CCWGG 6 cut(s) 411, 684, 699, 1066, 1327, 1466
AjuI GAANNNNNNNTTGG 2 cut(s) 1263, 1295
AluBI AGCT 9 cut(s) 236, 286, 362, 401, 521, 692, 754, 782, 820
AluI AGCT 9 cut(s) 236, 286, 362, 401, 521, 692, 754, 782, 820
Alw26I GTCTC 1 cut(s) 894
AlwI GGATC 3 cut(s) 459, 1057, 1327
AlwNI CAGNNNCTG 2 cut(s) 497, 1473
Ama87I CYCGRG 1 cut(s) 1047
AoxI GGCC 9 cut(s) 6, 93, 262, 540, 682, 826, 1182, 1442, 1464
ApeKI GCWGC 4 cut(s) 692, 695, 779, 1237
ApoI RAATTY 1 cut(s) 1095
AspLEI GCGC 2 cut(s) 740, 942
AspS9I GGNCC 3 cut(s) 847, 1443, 1464
AsuHPI GGTGA 2 cut(s) 1255, 1439
AvaI CYCGRG 1 cut(s) 1047
AvaII GGWCC 1 cut(s) 847
AxyI CCTNAGG 1 cut(s) 1155
BalI TGGCCA 1 cut(s) 684
BanII GRGCYC 2 cut(s) 107, 352
BauI CACGAG 2 cut(s) 1017, 1452
BbvI GCAGC 4 cut(s) 679, 707, 791, 1249
BccI CCATC 3 cut(s) 604, 752, 835
BceAI ACGGC 1 cut(s) 108
BciT130I CCWGG 6 cut(s) 413, 686, 701, 1068, 1329, 1468
BciVI GTATCC 1 cut(s) 568
BclI TGATCA 1 cut(s) 1363
BcoDI GTCTC 1 cut(s) 894
BfaI CTAG 4 cut(s) 147, 162, 522, 768
BfmI CTRYAG 1 cut(s) 693
BfoI RGCGCY 1 cut(s) 741
BfuAI ACCTGC 1 cut(s) 1231
BfuI GTATCC 1 cut(s) 568
BisI GCNGC 4 cut(s) 693, 696, 780, 1238
BlsI GCNGC 4 cut(s) 694, 697, 781, 1239
BmcAI AGTACT 1 cut(s) 606
Bme1390I CCNGG 6 cut(s) 413, 686, 701, 1068, 1329, 1468
Bme18I GGWCC 1 cut(s) 847
BmeT110I CYCGRG 1 cut(s) 1047
BmgT120I GGNCC 3 cut(s) 847, 1443, 1464
BmiI GGNNCC 4 cut(s) 327, 1039, 1374, 1465
BmrFI CCNGG 6 cut(s) 413, 686, 701, 1068, 1329, 1468
BmrI ACTGGG 2 cut(s) 110, 1440
BmsI GCATC 3 cut(s) 661, 940, 1445
BmuI ACTGGG 2 cut(s) 110, 1440
BplI GAGNNNNNCTC 2 cut(s) 891, 923
BpuEI CTTGAG 2 cut(s) 1200, 1364
BsaJI CCNNGG 5 cut(s) 309, 699, 829, 1066, 1412
BsaWI WCCGGW 1 cut(s) 448
Bsc4I CCNNNNNNNGG 3 cut(s) 1047, 1072, 1073
Bse1I ACTGG 6 cut(s) 105, 208, 258, 685, 739, 1446
Bse21I CCTNAGG 1 cut(s) 1155
Bse3DI GCAATG 3 cut(s) 681, 1152, 1311
BseBI CCWGG 6 cut(s) 413, 686, 701, 1068, 1329, 1468
BseDI CCNNGG 5 cut(s) 309, 699, 829, 1066, 1412
BseGI GGATG 4 cut(s) 619, 676, 763, 846
BseLI CCNNNNNNNGG 3 cut(s) 1047, 1072, 1073
BseMI GCAATG 3 cut(s) 681, 1152, 1311
BseMII CTCAG 1 cut(s) 1146
BseNI ACTGG 6 cut(s) 105, 208, 258, 685, 739, 1446
BseRI GAGGAG 2 cut(s) 551, 846
BseXI GCAGC 4 cut(s) 679, 707, 791, 1249
BsgI GTGCAG 1 cut(s) 1074
Bsh1236I CGCG 2 cut(s) 47, 438
Bsh1285I CGRYCG 2 cut(s) 24, 871
BshFI GGCC 9 cut(s) 8, 95, 264, 542, 684, 828, 1184, 1444, 1466
BsiEI CGRYCG 2 cut(s) 24, 871
BsiHKCI CYCGRG 1 cut(s) 1047
BsiSI CCGG 1 cut(s) 449
BslFI GGGAC 1 cut(s) 508
BslI CCNNNNNNNGG 3 cut(s) 1047, 1072, 1073
BsmAI GTCTC 1 cut(s) 894
BsmBI CGTCTC 1 cut(s) 894
BsmFI GGGAC 1 cut(s) 508
BsnI GGCC 9 cut(s) 8, 95, 264, 542, 684, 828, 1184, 1444, 1466
BsoBI CYCGRG 1 cut(s) 1047
Bsp1286I GDGCHC 2 cut(s) 107, 352
Bsp143I GATC 6 cut(s) 451, 868, 1062, 1319, 1363, 1403
BspACI CCGC 7 cut(s) 34, 45, 273, 438, 634, 838, 1314
BspANI GGCC 9 cut(s) 8, 95, 264, 542, 684, 828, 1184, 1444, 1466
BspCNI CTCAG 1 cut(s) 1147
BspFNI CGCG 2 cut(s) 47, 438
BspLI GGNNCC 4 cut(s) 327, 1039, 1374, 1465
BspMAI CTGCAG 1 cut(s) 697
BspMI ACCTGC 1 cut(s) 1231
BspPI GGATC 3 cut(s) 459, 1057, 1327
BspQI GCTCTTC 1 cut(s) 112
BsrBI CCGCTC 1 cut(s) 838
BsrDI GCAATG 3 cut(s) 681, 1152, 1311
BsrI ACTGG 6 cut(s) 105, 208, 258, 685, 739, 1446
BssECI CCNNGG 5 cut(s) 309, 699, 829, 1066, 1412
BssMI GATC 6 cut(s) 451, 868, 1062, 1319, 1363, 1403
BssSI CACGAG 2 cut(s) 1017, 1452
BssT1I CCWWGG 2 cut(s) 309, 1412
Bst2BI CACGAG 2 cut(s) 1017, 1452
Bst2UI CCWGG 6 cut(s) 413, 686, 701, 1068, 1329, 1468
Bst4CI ACNGT 2 cut(s) 25, 1266
Bst6I CTCTTC 3 cut(s) 112, 148, 291
BstC8I GCNNGC 1 cut(s) 399
BstDEI CTNAG 1 cut(s) 1155
BstF5I GGATG 4 cut(s) 619, 676, 763, 846
BstFNI CGCG 2 cut(s) 47, 438
BstH2I RGCGCY 1 cut(s) 741
BstHHI GCGC 2 cut(s) 740, 942
BstKTI GATC 6 cut(s) 454, 871, 1065, 1322, 1366, 1406
BstMAI GTCTC 1 cut(s) 894
BstMBI GATC 6 cut(s) 451, 868, 1062, 1319, 1363, 1403
BstMCI CGRYCG 2 cut(s) 24, 871
BstMWI GCNNNNNNNGC 2 cut(s) 817, 1385
BstNI CCWGG 6 cut(s) 413, 686, 701, 1068, 1329, 1468
BstSCI CCNGG 6 cut(s) 411, 684, 699, 1066, 1327, 1466
BstSFI CTRYAG 1 cut(s) 693
BstUI CGCG 2 cut(s) 47, 438
BstV1I GCAGC 4 cut(s) 679, 707, 791, 1249
BstX2I RGATCY 1 cut(s) 1319
BstXI CCANNNNNNTGG 1 cut(s) 419
BstYI RGATCY 1 cut(s) 1319
Bsu36I CCTNAGG 1 cut(s) 1155
BsuI GTATCC 1 cut(s) 568
BsuRI GGCC 9 cut(s) 8, 95, 264, 542, 684, 828, 1184, 1444, 1466
BtsCI GGATG 4 cut(s) 619, 676, 763, 846
BtsIMutI CAGTG 3 cut(s) 201, 265, 412
BveI ACCTGC 1 cut(s) 1231
Cac8I GCNNGC 1 cut(s) 399
CaiI CAGNNNCTG 2 cut(s) 497, 1473
CfoI GCGC 2 cut(s) 740, 942
Cfr13I GGNCC 3 cut(s) 847, 1443, 1464
CseI GACGC 1 cut(s) 891
Csp6I GTAC 4 cut(s) 11, 605, 981, 1383
CviAII CATG 1 cut(s) 1042
CviQI GTAC 4 cut(s) 11, 605, 981, 1383
DdeI CTNAG 1 cut(s) 1155
DpnI GATC 6 cut(s) 453, 870, 1064, 1321, 1365, 1405
DpnII GATC 6 cut(s) 451, 868, 1062, 1319, 1363, 1403
EaeI YGGCCR 4 cut(s) 93, 682, 826, 1182
Eam1104I CTCTTC 3 cut(s) 112, 148, 291
EarI CTCTTC 3 cut(s) 112, 148, 291
Eco130I CCWWGG 2 cut(s) 309, 1412
Eco24I GRGCYC 2 cut(s) 107, 352
Eco47I GGWCC 1 cut(s) 847
Eco57I CTGAAG 2 cut(s) 575, 975
Eco81I CCTNAGG 1 cut(s) 1155
Eco88I CYCGRG 1 cut(s) 1047
EcoO109I RGGNCCY 1 cut(s) 847
EcoRII CCWGG 6 cut(s) 411, 684, 699, 1066, 1327, 1466
EcoT14I CCWWGG 2 cut(s) 309, 1412
EcoT38I GRGCYC 2 cut(s) 107, 352
ErhI CCWWGG 2 cut(s) 309, 1412
Esp3I CGTCTC 1 cut(s) 894
FaeI CATG 1 cut(s) 1045
FalI AAGNNNNNCTT 2 cut(s) 977, 1009
FaqI GGGAC 1 cut(s) 508
FatI CATG 1 cut(s) 1041
FauI CCCGC 2 cut(s) 41, 627
FbaI TGATCA 1 cut(s) 1363
Fnu4HI GCNGC 4 cut(s) 693, 696, 780, 1238
FokI GGATG 4 cut(s) 626, 683, 770, 853
FriOI GRGCYC 2 cut(s) 107, 352
Fsp4HI GCNGC 4 cut(s) 693, 696, 780, 1238
FspBI CTAG 4 cut(s) 147, 162, 522, 768
GlaI GCGC 2 cut(s) 739, 941
GluI GCNGC 4 cut(s) 693, 696, 780, 1238
HaeII RGCGCY 1 cut(s) 741
HaeIII GGCC 9 cut(s) 8, 95, 264, 542, 684, 828, 1184, 1444, 1466
HapII CCGG 1 cut(s) 449
HgaI GACGC 1 cut(s) 891
HhaI GCGC 2 cut(s) 740, 942
Hin1II CATG 1 cut(s) 1045
Hin6I GCGC 2 cut(s) 738, 940
HinP1I GCGC 2 cut(s) 738, 940
HindIII AAGCTT 1 cut(s) 818
HpaII CCGG 1 cut(s) 449
HphI GGTGA 2 cut(s) 1255, 1439
Hpy166II GTNNAC 2 cut(s) 114, 159
Hpy188I TCNGA 5 cut(s) 73, 90, 594, 1368, 1408
Hpy8I GTNNAC 2 cut(s) 114, 159
Hpy99I CGWCG 3 cut(s) 52, 94, 1404
HpyAV CCTTC 9 cut(s) 76, 381, 383, 519, 579, 916, 1072, 1304, 1363
HpyCH4III ACNGT 2 cut(s) 25, 1266
HpyCH4IV ACGT 1 cut(s) 903
HpyCH4V TGCA 7 cut(s) 397, 674, 695, 964, 1091, 1304, 1388
HpyF10VI GCNNNNNNNGC 2 cut(s) 817, 1385
HpyF3I CTNAG 1 cut(s) 1155
HpySE526I ACGT 1 cut(s) 903
Hsp92II CATG 1 cut(s) 1045
HspAI GCGC 2 cut(s) 738, 940
Ksp22I TGATCA 1 cut(s) 1363
Kzo9I GATC 6 cut(s) 451, 868, 1062, 1319, 1363, 1403
LguI GCTCTTC 1 cut(s) 112
LmnI GCTCC 4 cut(s) 124, 325, 776, 1378
Lsp1109I GCAGC 4 cut(s) 679, 707, 791, 1249
LweI GCATC 3 cut(s) 661, 940, 1445
MaeI CTAG 4 cut(s) 147, 162, 522, 768
MaeII ACGT 1 cut(s) 903
MaeIII GTNAC 5 cut(s) 196, 407, 703, 1081, 1427
MalI GATC 6 cut(s) 453, 870, 1064, 1321, 1365, 1405
MbiI CCGCTC 1 cut(s) 838
MboI GATC 6 cut(s) 451, 868, 1062, 1319, 1363, 1403
MboII GAAGA 9 cut(s) 21, 99, 135, 308, 562, 959, 968, 982, 1300
MfeI CAATTG 2 cut(s) 251, 458
MflI RGATCY 1 cut(s) 1319
MhlI GDGCHC 2 cut(s) 107, 352
MlsI TGGCCA 1 cut(s) 684
MluCI AATT 8 cut(s) 225, 251, 458, 638, 959, 1095, 1175, 1209
MluNI TGGCCA 1 cut(s) 684
MlyI GAGTC 4 cut(s) 450, 1039, 1229, 1424
MmeI TCCRAC 1 cut(s) 113
Mox20I TGGCCA 1 cut(s) 684
MscI TGGCCA 1 cut(s) 684
MseI TTAA 3 cut(s) 465, 746, 792
Msp20I TGGCCA 1 cut(s) 684
MspI CCGG 1 cut(s) 449
MspR9I CCNGG 6 cut(s) 413, 686, 701, 1068, 1329, 1468
MunI CAATTG 2 cut(s) 251, 458
MvaI CCWGG 6 cut(s) 413, 686, 701, 1068, 1329, 1468
MvnI CGCG 2 cut(s) 47, 438
MwoI GCNNNNNNNGC 2 cut(s) 817, 1385
NdeII GATC 6 cut(s) 451, 868, 1062, 1319, 1363, 1403
NlaIII CATG 1 cut(s) 1045
NlaIV GGNNCC 4 cut(s) 327, 1039, 1374, 1465
NmeAIII GCCGAG 3 cut(s) 115, 854, 1412
NmuCI GTSAC 3 cut(s) 407, 703, 1427
PaqCI CACCTGC 1 cut(s) 1231
PciSI GCTCTTC 1 cut(s) 112
PfeI GAWTC 7 cut(s) 74, 85, 269, 764, 1130, 1187, 1291
PkrI GCNGC 4 cut(s) 694, 697, 781, 1239
Ple19I CGATCG 1 cut(s) 871
PleI GAGTC 4 cut(s) 449, 1039, 1228, 1424
PpsI GAGTC 4 cut(s) 449, 1039, 1228, 1424
PpuMI RGGWCCY 1 cut(s) 847
Psp5II RGGWCCY 1 cut(s) 847
Psp6I CCWGG 6 cut(s) 411, 684, 699, 1066, 1327, 1466
PspGI CCWGG 6 cut(s) 411, 684, 699, 1066, 1327, 1466
PspN4I GGNNCC 4 cut(s) 327, 1039, 1374, 1465
PspPI GGNCC 3 cut(s) 847, 1443, 1464
PspPPI RGGWCCY 1 cut(s) 847
PstI CTGCAG 1 cut(s) 697
PstNI CAGNNNCTG 2 cut(s) 497, 1473
PsuI RGATCY 1 cut(s) 1319
PvuI CGATCG 1 cut(s) 871
RsaI GTAC 4 cut(s) 12, 606, 982, 1384
RsaNI GTAC 4 cut(s) 11, 605, 981, 1383
SapI GCTCTTC 1 cut(s) 112
SaqAI TTAA 3 cut(s) 465, 746, 792
SatI GCNGC 4 cut(s) 693, 696, 780, 1238
Sau3AI GATC 6 cut(s) 451, 868, 1062, 1319, 1363, 1403
Sau96I GGNCC 3 cut(s) 847, 1443, 1464
ScaI AGTACT 1 cut(s) 606
SchI GAGTC 4 cut(s) 450, 1039, 1229, 1424
ScrFI CCNGG 6 cut(s) 413, 686, 701, 1068, 1329, 1468
SduI GDGCHC 2 cut(s) 107, 352
SfaNI GCATC 3 cut(s) 661, 940, 1445
SfcI CTRYAG 1 cut(s) 693
SinI GGWCC 1 cut(s) 847
SmlI CTYRAG 2 cut(s) 1215, 1343
SmoI CTYRAG 2 cut(s) 1215, 1343
Sse9I AATT 8 cut(s) 225, 251, 458, 638, 959, 1095, 1175, 1209
SsiI CCGC 7 cut(s) 34, 45, 273, 438, 634, 838, 1314
SspI AATATT 1 cut(s) 319
SspMI CTAG 4 cut(s) 147, 162, 522, 768
StyD4I CCNGG 6 cut(s) 411, 684, 699, 1066, 1327, 1466
StyI CCWWGG 2 cut(s) 309, 1412
TaaI ACNGT 2 cut(s) 25, 1266
TaiI ACGT 1 cut(s) 906
TaqI TCGA 4 cut(s) 267, 918, 1099, 1439
TasI AATT 8 cut(s) 225, 251, 458, 638, 959, 1095, 1175, 1209
TatI WGTACW 2 cut(s) 604, 980
TfiI GAWTC 7 cut(s) 74, 85, 269, 764, 1130, 1187, 1291
Tru1I TTAA 3 cut(s) 465, 746, 792
Tru9I TTAA 3 cut(s) 465, 746, 792
TscAI CASTG 3 cut(s) 208, 265, 412
TseFI GTSAC 3 cut(s) 407, 703, 1427
TseI GCWGC 4 cut(s) 692, 695, 779, 1237
Tsp45I GTSAC 3 cut(s) 407, 703, 1427
TspDTI ATGAA 1 cut(s) 1301
TspRI CASTG 3 cut(s) 208, 265, 412
VpaK11BI GGWCC 1 cut(s) 847
XapI RAATTY 1 cut(s) 1095
XbaI TCTAGA 2 cut(s) 146, 767
XspI CTAG 4 cut(s) 147, 162, 522, 768
ZrmI AGTACT 1 cut(s) 606
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.