Rh3CG372000

Squamosa promoter-binding-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3C
OG0021327
Physical Location & Seq
Forward (+)
47791089 .. 47812179
21091 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3CG372000.1

Sequence Viewer

Length: 699 bp
ATGCCTTTGCAAATTGCAGCTAATAGTTCTGATCAAACGAAGGATCAGGATCTGCTATCTCACCCGATGAAGAGCCTAGCCAATCTTGCTGGTACAGTTGATGGGAGAAACATATATGCATTACTTCAGGCATCTCAAGGTTTACCTAACACTGGGTCTTGCATCAAGACTGCACAACAGGTTCCAAATACGGTTTCCAACATCTTTGAACCCAGTAGGCCTTCTGTTTCTGCCTCAAATATGGATGATTGTATTATTATTGAGGAACCTTTAAGGCCCATAGGACAGCGCCCGATATTACCTGCATCAGATATGCAAAAGCGAAGATCTTCAGTTGATGGCGATCTTGGAAGTCAAATTACTTCAGCATGCATAGTTTTTTTTTTTTTTTTCCTGATTGAAAAGCTAGTAGTGAATGTGTGTGGAGTCTTAACTGCTCTTTTTTTTCTGCAAAATATCAGAATCGCACAAACCGCATTGTTTTTAAACTCTTTGGAAAAGACCCGAATGAACTTCCATTTGTTTTGCAATCACAGGTTACTGTGTGCTCTAGAAGGGAAGTATCTAGCACAAGAAGCTTGTGATTACGTGATGGATGGTGCTGATACAACTGTTGAGCATGATGAGCTACAGTGCATCAGATTCTCCTGCTCTATACCAAATGTTATTGGGCGAGGATTCATTGAGTATATTGCATAA

Protein Analysis

232

Amino Acids

25.59

Weight (kDa)

5.34

Isoelectric Point (pI)

53.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ig_SPL7 PF26102 177 - 229 5.8e-14 SPL7-like, immunoglobulin-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0021327)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr1g0333221 RchiOBHm_Chr7g0225661
rosa_multiflora Rmu_sc0001214.1_g000047
rosa_samantha Rh3CG372000 Rh7DG377300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 310
AciI CCGC 1 cut(s) 474
AclWI GGATC 2 cut(s) 51, 57
AcuI CTGAAG 3 cut(s) 110, 315, 348
AfaI GTAC 1 cut(s) 94
AfiI CCNNNNNNNGG 1 cut(s) 152
AgsI TTSAA 2 cut(s) 209, 401
AjuI GAANNNNNNNTTGG 2 cut(s) 178, 210
AluBI AGCT 4 cut(s) 20, 406, 578, 628
AluI AGCT 4 cut(s) 20, 406, 578, 628
Alw21I GWGCWC 1 cut(s) 550
AlwI GGATC 2 cut(s) 51, 57
AlwNI CAGNNNCTG 1 cut(s) 52
AoxI GGCC 2 cut(s) 218, 275
ApeKI GCWGC 1 cut(s) 17
Asp700I GAANNNNTTC 1 cut(s) 328
AspLEI GCGC 1 cut(s) 291
AspS9I GGNCC 1 cut(s) 276
AsuHPI GGTGA 1 cut(s) 53
BaeI ACNNNNGTAYC 2 cut(s) 597, 630
BarI GAAGNNNNNNTAC 2 cut(s) 343, 375
Bbv12I GWGCWC 1 cut(s) 550
BbvI GCAGC 1 cut(s) 29
BccI CCATC 4 cut(s) 95, 332, 586, 590
BclI TGATCA 1 cut(s) 31
BfaI CTAG 4 cut(s) 77, 407, 551, 566
BfmI CTRYAG 1 cut(s) 629
BfoI RGCGCY 1 cut(s) 292
BfuAI ACCTGC 1 cut(s) 310
BglII AGATCT 1 cut(s) 326
BisI GCNGC 1 cut(s) 18
BlsI GCNGC 1 cut(s) 19
BmgT120I GGNCC 1 cut(s) 276
BmiI GGNNCC 2 cut(s) 183, 267
BmrI ACTGGG 2 cut(s) 162, 207
BmsI GCATC 4 cut(s) 140, 171, 314, 645
BmuI ACTGGG 2 cut(s) 162, 207
BpuEI CTTGAG 1 cut(s) 120
BsaAI YACGTR 1 cut(s) 589
BsaBI GATNNNNATC 2 cut(s) 48, 342
Bsc4I CCNNNNNNNGG 1 cut(s) 152
Bse1I ACTGG 2 cut(s) 157, 213
Bse8I GATNNNNATC 2 cut(s) 48, 342
BseGI GGATG 2 cut(s) 250, 601
BseJI GATNNNNATC 2 cut(s) 48, 342
BseLI CCNNNNNNNGG 1 cut(s) 152
BseNI ACTGG 2 cut(s) 157, 213
BseXI GCAGC 1 cut(s) 29
BsgI GTGCAG 1 cut(s) 156
BshFI GGCC 2 cut(s) 220, 277
BsiHKAI GWGCWC 1 cut(s) 550
BslI CCNNNNNNNGG 1 cut(s) 152
BsnI GGCC 2 cut(s) 220, 277
Bsp1286I GDGCHC 1 cut(s) 550
Bsp143I GATC 5 cut(s) 31, 43, 49, 326, 343
BspACI CCGC 1 cut(s) 474
BspANI GGCC 2 cut(s) 220, 277
BspLI GGNNCC 2 cut(s) 183, 267
BspMI ACCTGC 1 cut(s) 310
BspPI GGATC 2 cut(s) 51, 57
BspQI GCTCTTC 1 cut(s) 65
BsrI ACTGG 2 cut(s) 157, 213
BssMI GATC 5 cut(s) 31, 43, 49, 326, 343
Bst4CI ACNGT 5 cut(s) 97, 193, 543, 613, 633
Bst6I CTCTTC 1 cut(s) 65
BstBAI YACGTR 1 cut(s) 589
BstC8I GCNNGC 1 cut(s) 370
BstF5I GGATG 2 cut(s) 250, 601
BstH2I RGCGCY 1 cut(s) 292
BstHHI GCGC 1 cut(s) 291
BstKTI GATC 5 cut(s) 34, 46, 52, 329, 346
BstMBI GATC 5 cut(s) 31, 43, 49, 326, 343
BstMWI GCNNNNNNNGC 4 cut(s) 86, 473, 575, 625
BstNSI RCATGY 1 cut(s) 372
BstSFI CTRYAG 1 cut(s) 629
BstV1I GCAGC 1 cut(s) 29
BstX2I RGATCY 2 cut(s) 49, 326
BstYI RGATCY 2 cut(s) 49, 326
BsuRI GGCC 2 cut(s) 220, 277
BtsCI GGATG 2 cut(s) 250, 601
BtsIMutI CAGTG 2 cut(s) 150, 638
BveI ACCTGC 1 cut(s) 310
Cac8I GCNNGC 1 cut(s) 370
CaiI CAGNNNCTG 1 cut(s) 52
CfoI GCGC 1 cut(s) 291
Cfr13I GGNCC 1 cut(s) 276
Csp6I GTAC 1 cut(s) 93
CviAII CATG 2 cut(s) 369, 620
CviJI RGCY 8 cut(s) 20, 75, 80, 220, 277, 406, 578, 628
CviKI_1 RGCY 8 cut(s) 20, 75, 80, 220, 277, 406, 578, 628
CviQI GTAC 1 cut(s) 93
DpnI GATC 5 cut(s) 33, 45, 51, 328, 345
DpnII GATC 5 cut(s) 31, 43, 49, 326, 343
DraI TTTAAA 1 cut(s) 486
Eam1104I CTCTTC 1 cut(s) 65
EarI CTCTTC 1 cut(s) 65
Eco147I AGGCCT 1 cut(s) 220
Eco57I CTGAAG 3 cut(s) 110, 315, 348
EcoT22I ATGCAT 2 cut(s) 121, 374
FaeI CATG 2 cut(s) 372, 623
FatI CATG 2 cut(s) 368, 619
FbaI TGATCA 1 cut(s) 31
Fnu4HI GCNGC 1 cut(s) 18
FokI GGATG 2 cut(s) 257, 608
Fsp4HI GCNGC 1 cut(s) 18
FspBI CTAG 4 cut(s) 77, 407, 551, 566
GlaI GCGC 1 cut(s) 290
GluI GCNGC 1 cut(s) 18
HaeII RGCGCY 1 cut(s) 292
HaeIII GGCC 2 cut(s) 220, 277
HhaI GCGC 1 cut(s) 291
Hin1II CATG 2 cut(s) 372, 623
Hin6I GCGC 1 cut(s) 289
HinP1I GCGC 1 cut(s) 289
HindIII AAGCTT 1 cut(s) 576
HinfI GANTC 4 cut(s) 426, 462, 642, 678
HphI GGTGA 1 cut(s) 53
Hpy166II GTNNAC 1 cut(s) 143
Hpy188I TCNGA 4 cut(s) 31, 310, 461, 641
Hpy188III TCNNGA 4 cut(s) 47, 166, 394, 551
Hpy8I GTNNAC 1 cut(s) 143
HpyAV CCTTC 3 cut(s) 34, 231, 548
HpyCH4III ACNGT 5 cut(s) 97, 193, 543, 613, 633
HpyCH4IV ACGT 1 cut(s) 588
HpyF10VI GCNNNNNNNGC 4 cut(s) 86, 473, 575, 625
HpySE526I ACGT 1 cut(s) 588
Hsp92II CATG 2 cut(s) 372, 623
HspAI GCGC 1 cut(s) 289
Ksp22I TGATCA 1 cut(s) 31
Kzo9I GATC 5 cut(s) 31, 43, 49, 326, 343
LguI GCTCTTC 1 cut(s) 65
Lsp1109I GCAGC 1 cut(s) 29
LweI GCATC 4 cut(s) 140, 171, 314, 645
MaeI CTAG 4 cut(s) 77, 407, 551, 566
MaeII ACGT 1 cut(s) 588
MaeIII GTNAC 1 cut(s) 537
MalI GATC 5 cut(s) 33, 45, 51, 328, 345
MboI GATC 5 cut(s) 31, 43, 49, 326, 343
MboII GAAGA 3 cut(s) 82, 321, 336
MflI RGATCY 2 cut(s) 49, 326
MhlI GDGCHC 1 cut(s) 550
MluCI AATT 2 cut(s) 12, 357
MlyI GAGTC 1 cut(s) 435
MmeI TCCRAC 1 cut(s) 222
MnlI CCTC 3 cut(s) 244, 256, 668
Mph1103I ATGCAT 2 cut(s) 121, 374
MroXI GAANNNNTTC 1 cut(s) 328
MseI TTAA 3 cut(s) 272, 431, 485
MwoI GCNNNNNNNGC 4 cut(s) 86, 473, 575, 625
NdeII GATC 5 cut(s) 31, 43, 49, 326, 343
NlaIII CATG 2 cut(s) 372, 623
NlaIV GGNNCC 2 cut(s) 183, 267
NsiI ATGCAT 2 cut(s) 121, 374
NspI RCATGY 1 cut(s) 372
PaeI GCATGC 1 cut(s) 372
PceI AGGCCT 1 cut(s) 220
PciSI GCTCTTC 1 cut(s) 65
PdmI GAANNNNTTC 1 cut(s) 328
PfeI GAWTC 3 cut(s) 462, 642, 678
PkrI GCNGC 1 cut(s) 19
PleI GAGTC 1 cut(s) 434
PpsI GAGTC 1 cut(s) 434
Ppu21I YACGTR 1 cut(s) 589
PspN4I GGNNCC 2 cut(s) 183, 267
PspPI GGNCC 1 cut(s) 276
PstNI CAGNNNCTG 1 cut(s) 52
PsuI RGATCY 2 cut(s) 49, 326
RsaI GTAC 1 cut(s) 94
RsaNI GTAC 1 cut(s) 93
SapI GCTCTTC 1 cut(s) 65
SaqAI TTAA 3 cut(s) 272, 431, 485
SatI GCNGC 1 cut(s) 18
Sau3AI GATC 5 cut(s) 31, 43, 49, 326, 343
Sau96I GGNCC 1 cut(s) 276
SchI GAGTC 1 cut(s) 435
SduI GDGCHC 1 cut(s) 550
SfaNI GCATC 4 cut(s) 140, 171, 314, 645
SfcI CTRYAG 1 cut(s) 629
SmlI CTYRAG 1 cut(s) 135
SmoI CTYRAG 1 cut(s) 135
SphI GCATGC 1 cut(s) 372
Sse9I AATT 2 cut(s) 12, 357
SseBI AGGCCT 1 cut(s) 220
SsiI CCGC 1 cut(s) 474
SspMI CTAG 4 cut(s) 77, 407, 551, 566
StuI AGGCCT 1 cut(s) 220
TaaI ACNGT 5 cut(s) 97, 193, 543, 613, 633
TaiI ACGT 1 cut(s) 591
TasI AATT 2 cut(s) 12, 357
TfiI GAWTC 3 cut(s) 462, 642, 678
Tru1I TTAA 3 cut(s) 272, 431, 485
Tru9I TTAA 3 cut(s) 272, 431, 485
TscAI CASTG 2 cut(s) 157, 638
TseI GCWGC 1 cut(s) 17
TspDTI ATGAA 3 cut(s) 83, 524, 670
TspRI CASTG 2 cut(s) 157, 638
XbaI TCTAGA 1 cut(s) 550
XceI RCATGY 1 cut(s) 372
XmnI GAANNNNTTC 1 cut(s) 328
XspI CTAG 4 cut(s) 77, 407, 551, 566
Zsp2I ATGCAT 2 cut(s) 121, 374
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.