Rh4AG014600

F-box kelch-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0019194
Physical Location & Seq
Forward (+)
2803546 .. 2805858
2313 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG014600.1

Sequence Viewer

Length: 891 bp
ATGGAATTGCCGGATCTGCCGAGTGAGATGTGGGAATGCATATTCCTGCATGCTAAACTGTCTGTGAAAACTCTAGTCCAGTTGAGTTGCTTTTGCAGGGTATGGCGTTTGGCAATTTTCTCCCCAACATTTATCAAGGCATACGATGAAAGTGATAGTGGCAAGACTGGTTTATTGCTTTTTCAAACTCAACTGAAGTTGGTTTACTGGCTGTTCTCGACTGATATCAATAACTGGGTTTCCTTTATAAACAGTCCTTTGGAGATTCGGGGCCTTTCAATCGTTGGCTCTTGCAGGGGACTCATTTGTTTCAGTGAGCCCTATGGGATGATTTTAAATCCACCAATATATGTCTGGAATCCTTCCATTCGGAGACTGAGGCAGTTACCACAGGGACATATCCAAATAGAAGAAGGGGATCAAGATGGTGATGTTATTGTTCCTACTGCCATTGGTCTAGGATTTGATGCTAAGAGATATGACTATAAAGTGGTTAAAATAAGGTGCACTGGATCGACGGAGATGATGAATCCTTTGTATGAAGTCGAAGAGTACAGTCTGAGGTCCAATGTTTGGACAAGGTTGCCGCCACTGAATGTCGCACTTGGTGATAATGAAACCATACTTTTTCACAGCAGCGATGGCATTTACTTGTCAGGCTTTGTGTACTGGATGCTTGCCCGTAACAATGAAGGCATGATGTGCTATGATCTGGCAGATCAAAGATTTACAGTGCAAGCATATCCAAAGCCTCCACTTTTTCAGAATGATCATCAAATTTCGGCTGGAGTTCATCATCTATCACTTTCATTTCTCACTTGGAGGCAGCAAGATGGTTATTTTATTTGTCAAGTCCACACCAAGCAGAAAAACGAGGAAGACCAAGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box FBA_1

Protein Analysis

296

Amino Acids

34.14

Weight (kDa)

5.41

Isoelectric Point (pI)

43.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FBA_1 PF07734 92 - 249 1.1e-11 F-box associated beta propeller domain
FBA_3 PF08268 92 - 247 9e-08 F-box associated beta propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019194)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g16010
rosa_laevigata RLG00000017434
rosa_multiflora Rmu_sc0001942.1_g000028
rosa_samantha Rh2CG184000 Rh2CG184100 Rh4AG014600 Rh4DG010200
rosa_wichuraiana Rw2G014060

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 248
AccB7I CCANNNNNTGG 1 cut(s) 573
AciI CCGC 1 cut(s) 587
AclWI GGATC 3 cut(s) 21, 426, 520
AcsI RAATTY 1 cut(s) 777
AcuI CTGAAG 1 cut(s) 215
AdeI CACNNNGTG 1 cut(s) 608
AfaI GTAC 2 cut(s) 554, 668
AfiI CCNNNNNNNGG 1 cut(s) 573
AgsI TTSAA 2 cut(s) 185, 279
Alw21I GWGCWC 1 cut(s) 509
Alw26I GTCTC 1 cut(s) 367
Alw44I GTGCAC 1 cut(s) 505
AlwI GGATC 3 cut(s) 21, 426, 520
AoxI GGCC 1 cut(s) 271
ApaLI GTGCAC 1 cut(s) 505
ApeKI GCWGC 2 cut(s) 636, 826
ApoI RAATTY 1 cut(s) 777
AspS9I GGNCC 2 cut(s) 271, 564
AsuHPI GGTGA 2 cut(s) 440, 620
AvaII GGWCC 1 cut(s) 564
BaeGI GKGCMC 1 cut(s) 509
BanII GRGCYC 1 cut(s) 321
BarI GAAGNNNNNNTAC 2 cut(s) 188, 220
BbsI GAAGAC 1 cut(s) 885
Bbv12I GWGCWC 1 cut(s) 509
BbvI GCAGC 2 cut(s) 648, 838
BccI CCATC 3 cut(s) 419, 635, 827
BclI TGATCA 1 cut(s) 769
BcoDI GTCTC 1 cut(s) 367
BfaI CTAG 2 cut(s) 74, 458
BisI GCNGC 3 cut(s) 587, 637, 827
BlsI GCNGC 3 cut(s) 588, 638, 828
Bme18I GGWCC 1 cut(s) 564
BmgT120I GGNCC 2 cut(s) 271, 564
BmiI GGNNCC 1 cut(s) 272
BmrI ACTGGG 1 cut(s) 244
BmsI GCATC 2 cut(s) 457, 663
BmuI ACTGGG 1 cut(s) 244
BpiI GAAGAC 1 cut(s) 885
BpmI CTGGAG 1 cut(s) 807
Bsc4I CCNNNNNNNGG 1 cut(s) 573
Bse1I ACTGG 6 cut(s) 79, 172, 212, 239, 514, 674
BseGI GGATG 2 cut(s) 333, 678
BseLI CCNNNNNNNGG 1 cut(s) 573
BseMII CTCAG 2 cut(s) 368, 551
BseNI ACTGG 6 cut(s) 79, 172, 212, 239, 514, 674
BseSI GKGCMC 1 cut(s) 509
BseXI GCAGC 2 cut(s) 648, 838
BshFI GGCC 1 cut(s) 273
BsiHKAI GWGCWC 1 cut(s) 509
BsiSI CCGG 1 cut(s) 11
BslFI GGGAC 2 cut(s) 312, 408
BslI CCNNNNNNNGG 1 cut(s) 573
BsmAI GTCTC 1 cut(s) 367
BsmFI GGGAC 2 cut(s) 312, 408
BsmI GAATGC 1 cut(s) 41
BsnI GGCC 1 cut(s) 273
Bsp1286I GDGCHC 2 cut(s) 321, 509
Bsp143I GATC 6 cut(s) 13, 418, 512, 709, 718, 769
BspACI CCGC 1 cut(s) 587
BspANI GGCC 1 cut(s) 273
BspCNI CTCAG 2 cut(s) 369, 552
BspLI GGNNCC 1 cut(s) 272
BspPI GGATC 3 cut(s) 21, 426, 520
BsrI ACTGG 6 cut(s) 79, 172, 212, 239, 514, 674
BssMI GATC 6 cut(s) 13, 418, 512, 709, 718, 769
Bst4CI ACNGT 4 cut(s) 60, 254, 557, 733
Bst6I CTCTTC 1 cut(s) 543
BstAPI GCANNNNNTGC 1 cut(s) 702
BstC8I GCNNGC 3 cut(s) 51, 678, 738
BstDEI CTNAG 3 cut(s) 377, 471, 560
BstF5I GGATG 2 cut(s) 333, 678
BstKTI GATC 6 cut(s) 16, 421, 515, 712, 721, 772
BstMAI GTCTC 1 cut(s) 367
BstMBI GATC 6 cut(s) 13, 418, 512, 709, 718, 769
BstMWI GCNNNNNNNGC 3 cut(s) 16, 642, 702
BstNSI RCATGY 1 cut(s) 53
BstSLI GKGCMC 1 cut(s) 509
BstV1I GCAGC 2 cut(s) 648, 838
BstV2I GAAGAC 1 cut(s) 885
BstX2I RGATCY 1 cut(s) 13
BstYI RGATCY 1 cut(s) 13
BsuRI GGCC 1 cut(s) 273
BtgZI GCGATG 1 cut(s) 654
BtsCI GGATG 2 cut(s) 333, 678
BtsIMutI CAGTG 4 cut(s) 319, 507, 590, 738
Cac8I GCNNGC 3 cut(s) 51, 678, 738
Cfr13I GGNCC 2 cut(s) 271, 564
Csp6I GTAC 2 cut(s) 553, 667
CviAII CATG 2 cut(s) 50, 697
CviJI RGCY 7 cut(s) 211, 273, 288, 319, 660, 751, 785
CviKI_1 RGCY 7 cut(s) 211, 273, 288, 319, 660, 751, 785
CviQI GTAC 2 cut(s) 553, 667
DdeI CTNAG 3 cut(s) 377, 471, 560
DpnI GATC 6 cut(s) 15, 420, 514, 711, 720, 771
DpnII GATC 6 cut(s) 13, 418, 512, 709, 718, 769
DraI TTTAAA 1 cut(s) 336
DraIII CACNNNGTG 1 cut(s) 608
Eam1104I CTCTTC 1 cut(s) 543
EarI CTCTTC 1 cut(s) 543
Eco24I GRGCYC 1 cut(s) 321
Eco32I GATATC 1 cut(s) 226
Eco47I GGWCC 1 cut(s) 564
Eco57I CTGAAG 1 cut(s) 215
EcoO109I RGGNCCY 1 cut(s) 271
EcoRV GATATC 1 cut(s) 226
EcoT22I ATGCAT 1 cut(s) 41
EcoT38I GRGCYC 1 cut(s) 321
FaeI CATG 2 cut(s) 53, 700
FaqI GGGAC 2 cut(s) 312, 408
FatI CATG 2 cut(s) 49, 696
FbaI TGATCA 1 cut(s) 769
Fnu4HI GCNGC 3 cut(s) 587, 637, 827
FokI GGATG 2 cut(s) 340, 685
FriOI GRGCYC 1 cut(s) 321
Fsp4HI GCNGC 3 cut(s) 587, 637, 827
FspBI CTAG 2 cut(s) 74, 458
GluI GCNGC 3 cut(s) 587, 637, 827
GsuI CTGGAG 1 cut(s) 807
HaeIII GGCC 1 cut(s) 273
HapII CCGG 1 cut(s) 11
Hin1II CATG 2 cut(s) 53, 700
HinfI GANTC 4 cut(s) 265, 300, 358, 529
HpaII CCGG 1 cut(s) 11
HphI GGTGA 2 cut(s) 440, 620
Hpy166II GTNNAC 4 cut(s) 205, 507, 667, 856
Hpy188I TCNGA 3 cut(s) 372, 561, 765
Hpy188III TCNNGA 3 cut(s) 217, 355, 422
Hpy8I GTNNAC 4 cut(s) 205, 507, 667, 856
Hpy99I CGWCG 1 cut(s) 520
HpyAV CCTTC 3 cut(s) 372, 407, 686
HpyCH4III ACNGT 4 cut(s) 60, 254, 557, 733
HpyCH4V TGCA 6 cut(s) 39, 49, 96, 294, 507, 736
HpyF10VI GCNNNNNNNGC 3 cut(s) 16, 642, 702
HpyF3I CTNAG 3 cut(s) 377, 471, 560
Hsp92II CATG 2 cut(s) 53, 700
Ksp22I TGATCA 1 cut(s) 769
Kzo9I GATC 6 cut(s) 13, 418, 512, 709, 718, 769
Lsp1109I GCAGC 2 cut(s) 648, 838
LweI GCATC 2 cut(s) 457, 663
MaeI CTAG 2 cut(s) 74, 458
MaeIII GTNAC 2 cut(s) 384, 683
MalI GATC 6 cut(s) 15, 420, 514, 711, 720, 771
MboI GATC 6 cut(s) 13, 418, 512, 709, 718, 769
MboII GAAGA 3 cut(s) 422, 560, 890
MflI RGATCY 1 cut(s) 13
MhlI GDGCHC 2 cut(s) 321, 509
MluCI AATT 3 cut(s) 5, 114, 777
MlyI GAGTC 1 cut(s) 294
MnlI CCTC 5 cut(s) 372, 555, 762, 816, 868
Mph1103I ATGCAT 1 cut(s) 41
MseI TTAA 2 cut(s) 335, 495
MspI CCGG 1 cut(s) 11
Mva1269I GAATGC 1 cut(s) 41
MwoI GCNNNNNNNGC 3 cut(s) 16, 642, 702
NdeII GATC 6 cut(s) 13, 418, 512, 709, 718, 769
NlaIII CATG 2 cut(s) 53, 700
NlaIV GGNNCC 1 cut(s) 272
NmeAIII GCCGAG 1 cut(s) 45
NsiI ATGCAT 1 cut(s) 41
NspI RCATGY 1 cut(s) 53
PaeI GCATGC 1 cut(s) 53
PctI GAATGC 1 cut(s) 41
PfeI GAWTC 3 cut(s) 265, 358, 529
PflMI CCANNNNNTGG 1 cut(s) 573
PkrI GCNGC 3 cut(s) 588, 638, 828
PleI GAGTC 1 cut(s) 294
PpsI GAGTC 1 cut(s) 294
PsiI TTATAA 1 cut(s) 248
PspN4I GGNNCC 1 cut(s) 272
PspPI GGNCC 2 cut(s) 271, 564
PsuI RGATCY 1 cut(s) 13
RsaI GTAC 2 cut(s) 554, 668
RsaNI GTAC 2 cut(s) 553, 667
SaqAI TTAA 2 cut(s) 335, 495
SatI GCNGC 3 cut(s) 587, 637, 827
Sau3AI GATC 6 cut(s) 13, 418, 512, 709, 718, 769
Sau96I GGNCC 2 cut(s) 271, 564
SchI GAGTC 1 cut(s) 294
SduI GDGCHC 2 cut(s) 321, 509
SetI ASST 3 cut(s) 506, 566, 584
SfaNI GCATC 2 cut(s) 457, 663
SinI GGWCC 1 cut(s) 564
SphI GCATGC 1 cut(s) 53
Sse9I AATT 3 cut(s) 5, 114, 777
SsiI CCGC 1 cut(s) 587
SspMI CTAG 2 cut(s) 74, 458
TaaI ACNGT 4 cut(s) 60, 254, 557, 733
TaqI TCGA 3 cut(s) 218, 515, 546
TasI AATT 3 cut(s) 5, 114, 777
TatI WGTACW 2 cut(s) 552, 666
TauI GCSGC 1 cut(s) 589
TfiI GAWTC 3 cut(s) 265, 358, 529
Tru1I TTAA 2 cut(s) 335, 495
Tru9I TTAA 2 cut(s) 335, 495
TscAI CASTG 4 cut(s) 319, 514, 597, 738
TseI GCWGC 2 cut(s) 636, 826
TspDTI ATGAA 7 cut(s) 162, 542, 555, 630, 705, 782, 798
TspGWI ACGGA 1 cut(s) 533
TspRI CASTG 4 cut(s) 319, 514, 597, 738
Van91I CCANNNNNTGG 1 cut(s) 573
VneI GTGCAC 1 cut(s) 505
VpaK11BI GGWCC 1 cut(s) 564
XapI RAATTY 1 cut(s) 777
XceI RCATGY 1 cut(s) 53
XcmI CCANNNNNNNNNTGG 1 cut(s) 351
XspI CTAG 2 cut(s) 74, 458
Zsp2I ATGCAT 1 cut(s) 41
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.