Rh4AG175700

macrophage migration inhibitory factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0019403
Physical Location & Seq
Reverse (-)
44060524 .. 44062985
2462 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG175700.1

Sequence Viewer

Length: 225 bp
ATGGATGCAGTAATAGCCTCTGATATTCTCAAGGATGCCACCAAAGTCGTTGCCAAAATTATCGGCAAACCCGAGTCCTATGTGATGATTTTGCTCAATAGGATTGTGCAGATTTCATTTGCGGGCACCAAAGAGCAGGTTGCGTATGGGGAATTGATCTCCATTTGGGGCATTGGACCAAGTGTTAATGGGAAGCTGATTCTGGAGACCATGCCAAGGAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00350 ko00360 map00350 map00360
Pfam Domains
Protein Families
MIF

Protein Analysis

74

Amino Acids

8.04

Weight (kDa)

9.1

Isoelectric Point (pI)

14.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MIF PF01187 4 - 64 3.2e-08 Macrophage migration inhibitory factor (MIF)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 127
AccB1I GGYRCC 1 cut(s) 125
AciI CCGC 1 cut(s) 122
AfiI CCNNNNNNNGG 1 cut(s) 216
AluBI AGCT 1 cut(s) 196
AluI AGCT 1 cut(s) 196
Alw26I GTCTC 1 cut(s) 200
Ama87I CYCGRG 1 cut(s) 71
AspS9I GGNCC 1 cut(s) 176
AvaI CYCGRG 1 cut(s) 71
AvaII GGWCC 1 cut(s) 176
BaeGI GKGCMC 1 cut(s) 128
BanI GGYRCC 1 cut(s) 125
BcoDI GTCTC 1 cut(s) 200
BfuAI ACCTGC 1 cut(s) 127
Bme18I GGWCC 1 cut(s) 176
BmeT110I CYCGRG 1 cut(s) 71
BmgT120I GGNCC 1 cut(s) 176
BmiI GGNNCC 1 cut(s) 127
BmsI GCATC 1 cut(s) 25
BpmI CTGGAG 1 cut(s) 224
BpuEI CTTGAG 1 cut(s) 14
BsaI GGTCTC 1 cut(s) 200
BsaJI CCNNGG 1 cut(s) 215
Bsc4I CCNNNNNNNGG 1 cut(s) 216
BseDI CCNNGG 1 cut(s) 215
BseGI GGATG 2 cut(s) 10, 40
BseLI CCNNNNNNNGG 1 cut(s) 216
BseSI GKGCMC 1 cut(s) 128
BsgI GTGCAG 1 cut(s) 128
BshNI GGYRCC 1 cut(s) 125
BsiHKCI CYCGRG 1 cut(s) 71
BslI CCNNNNNNNGG 1 cut(s) 216
BsmAI GTCTC 1 cut(s) 200
Bso31I GGTCTC 1 cut(s) 200
BsoBI CYCGRG 1 cut(s) 71
Bsp1286I GDGCHC 1 cut(s) 128
Bsp143I GATC 1 cut(s) 156
BspACI CCGC 1 cut(s) 122
BspLI GGNNCC 1 cut(s) 127
BspMI ACCTGC 1 cut(s) 127
BspT107I GGYRCC 1 cut(s) 125
BspTNI GGTCTC 1 cut(s) 200
BssECI CCNNGG 1 cut(s) 215
BssMI GATC 1 cut(s) 156
BssT1I CCWWGG 1 cut(s) 215
BstC8I GCNNGC 1 cut(s) 124
BstF5I GGATG 2 cut(s) 10, 40
BstKTI GATC 1 cut(s) 159
BstMAI GTCTC 1 cut(s) 200
BstMBI GATC 1 cut(s) 156
BstMWI GCNNNNNNNGC 1 cut(s) 14
BstSLI GKGCMC 1 cut(s) 128
BtsCI GGATG 2 cut(s) 10, 40
BveI ACCTGC 1 cut(s) 127
Cac8I GCNNGC 1 cut(s) 124
Cfr13I GGNCC 1 cut(s) 176
CviAII CATG 1 cut(s) 211
CviJI RGCY 2 cut(s) 17, 196
CviKI_1 RGCY 2 cut(s) 17, 196
DpnI GATC 1 cut(s) 158
DpnII GATC 1 cut(s) 156
Eco130I CCWWGG 1 cut(s) 215
Eco31I GGTCTC 1 cut(s) 200
Eco47I GGWCC 1 cut(s) 176
Eco88I CYCGRG 1 cut(s) 71
EcoT14I CCWWGG 1 cut(s) 215
ErhI CCWWGG 1 cut(s) 215
FaeI CATG 1 cut(s) 214
FaiI YATR 3 cut(s) 81, 147, 212
FatI CATG 1 cut(s) 210
FauI CCCGC 1 cut(s) 115
FokI GGATG 2 cut(s) 17, 47
GsuI CTGGAG 1 cut(s) 224
Hin1II CATG 1 cut(s) 214
HinfI GANTC 2 cut(s) 74, 199
Hpy188I TCNGA 1 cut(s) 22
Hpy188III TCNNGA 1 cut(s) 203
HpyCH4V TGCA 2 cut(s) 8, 109
HpyF10VI GCNNNNNNNGC 1 cut(s) 14
Hsp92II CATG 1 cut(s) 214
Kzo9I GATC 1 cut(s) 156
LpnPI CCDG 2 cut(s) 122, 188
LweI GCATC 1 cut(s) 25
MalI GATC 1 cut(s) 158
MboI GATC 1 cut(s) 156
MhlI GDGCHC 1 cut(s) 128
MluCI AATT 2 cut(s) 57, 152
MlyI GAGTC 1 cut(s) 83
MnlI CCTC 1 cut(s) 28
MseI TTAA 1 cut(s) 186
MwoI GCNNNNNNNGC 1 cut(s) 14
NdeII GATC 1 cut(s) 156
NlaIII CATG 1 cut(s) 214
NlaIV GGNNCC 1 cut(s) 127
PcsI WCGNNNNNNNCGW 1 cut(s) 69
PfeI GAWTC 1 cut(s) 199
PleI GAGTC 1 cut(s) 82
PpsI GAGTC 1 cut(s) 82
PspN4I GGNNCC 1 cut(s) 127
PspPI GGNCC 1 cut(s) 176
SaqAI TTAA 1 cut(s) 186
Sau3AI GATC 1 cut(s) 156
Sau96I GGNCC 1 cut(s) 176
SchI GAGTC 1 cut(s) 83
SduI GDGCHC 1 cut(s) 128
SetI ASST 2 cut(s) 141, 198
SfaNI GCATC 1 cut(s) 25
SgeI CNNG 7 cut(s) 43, 83, 85, 135, 149, 192, 215
SinI GGWCC 1 cut(s) 176
SmlI CTYRAG 1 cut(s) 29
SmoI CTYRAG 1 cut(s) 29
Sse9I AATT 2 cut(s) 57, 152
SsiI CCGC 1 cut(s) 122
StyI CCWWGG 1 cut(s) 215
TasI AATT 2 cut(s) 57, 152
TfiI GAWTC 1 cut(s) 199
Tru1I TTAA 1 cut(s) 186
Tru9I TTAA 1 cut(s) 186
TspDTI ATGAA 1 cut(s) 105
VpaK11BI GGWCC 1 cut(s) 176
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.