Rh4BG175900

Macrophage migration inhibitory factor homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0019403
Physical Location & Seq
Reverse (-)
31265398 .. 31265674
277 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG175900.1

Sequence Viewer

Length: 225 bp
ATGGATGCAGTAATAGCCTCTGATATTCTCAAGGATGCCACCGAAGTCGCTGCCAAAATTATCGGCAAACCCGAGTCCTATGTGATGATTTTGCTTAATGGGATTGTGCCGATTTCATTTGCGGGCACCAAAGAGCCGGTTGCGTATGGTGAATTGATCTCCATTTGGGGCATTGGACCGAGTGTTAATGGGAAGCTGATTCTGGAGACCATGCCAAGTGTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00350 ko00360 map00350 map00360
Pfam Domains
Protein Families
MIF

Protein Analysis

74

Amino Acids

7.76

Weight (kDa)

4.56

Isoelectric Point (pI)

25.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MIF PF01187 4 - 64 1.8e-09 Macrophage migration inhibitory factor (MIF)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 125
AciI CCGC 1 cut(s) 122
AluBI AGCT 1 cut(s) 196
AluI AGCT 1 cut(s) 196
Alw26I GTCTC 1 cut(s) 200
Ama87I CYCGRG 1 cut(s) 71
ApeKI GCWGC 1 cut(s) 50
AspS9I GGNCC 1 cut(s) 176
AsuHPI GGTGA 1 cut(s) 161
AvaI CYCGRG 1 cut(s) 71
AvaII GGWCC 1 cut(s) 176
BaeGI GKGCMC 1 cut(s) 128
BanI GGYRCC 1 cut(s) 125
BbvI GCAGC 1 cut(s) 37
BcgI CGANNNNNNTGC 2 cut(s) 32, 66
BcoDI GTCTC 1 cut(s) 200
BisI GCNGC 1 cut(s) 51
BlsI GCNGC 1 cut(s) 52
Bme18I GGWCC 1 cut(s) 176
BmeT110I CYCGRG 1 cut(s) 71
BmgT120I GGNCC 1 cut(s) 176
BmiI GGNNCC 1 cut(s) 127
BmsI GCATC 1 cut(s) 25
BpmI CTGGAG 1 cut(s) 224
BpuEI CTTGAG 1 cut(s) 14
BsaI GGTCTC 1 cut(s) 200
Bse118I RCCGGY 1 cut(s) 136
BseGI GGATG 2 cut(s) 10, 40
BseSI GKGCMC 1 cut(s) 128
BseXI GCAGC 1 cut(s) 37
BshNI GGYRCC 1 cut(s) 125
BsiHKCI CYCGRG 1 cut(s) 71
BsiSI CCGG 1 cut(s) 137
BsmAI GTCTC 1 cut(s) 200
Bso31I GGTCTC 1 cut(s) 200
BsoBI CYCGRG 1 cut(s) 71
Bsp1286I GDGCHC 1 cut(s) 128
Bsp143I GATC 1 cut(s) 156
BspACI CCGC 1 cut(s) 122
BspLI GGNNCC 1 cut(s) 127
BspT107I GGYRCC 1 cut(s) 125
BspTNI GGTCTC 1 cut(s) 200
BsrFI RCCGGY 1 cut(s) 136
BssAI RCCGGY 1 cut(s) 136
BssMI GATC 1 cut(s) 156
BstC8I GCNNGC 1 cut(s) 124
BstF5I GGATG 2 cut(s) 10, 40
BstKTI GATC 1 cut(s) 159
BstMAI GTCTC 1 cut(s) 200
BstMBI GATC 1 cut(s) 156
BstMWI GCNNNNNNNGC 1 cut(s) 14
BstSLI GKGCMC 1 cut(s) 128
BstV1I GCAGC 1 cut(s) 37
BtsCI GGATG 2 cut(s) 10, 40
Cac8I GCNNGC 1 cut(s) 124
Cfr10I RCCGGY 1 cut(s) 136
Cfr13I GGNCC 1 cut(s) 176
CviAII CATG 1 cut(s) 211
CviJI RGCY 3 cut(s) 17, 136, 196
CviKI_1 RGCY 3 cut(s) 17, 136, 196
DpnI GATC 1 cut(s) 158
DpnII GATC 1 cut(s) 156
Eco31I GGTCTC 1 cut(s) 200
Eco47I GGWCC 1 cut(s) 176
Eco88I CYCGRG 1 cut(s) 71
FaeI CATG 1 cut(s) 214
FaiI YATR 3 cut(s) 81, 147, 212
FatI CATG 1 cut(s) 210
FauI CCCGC 1 cut(s) 115
Fnu4HI GCNGC 1 cut(s) 51
FokI GGATG 2 cut(s) 17, 47
Fsp4HI GCNGC 1 cut(s) 51
GluI GCNGC 1 cut(s) 51
GsuI CTGGAG 1 cut(s) 224
HapII CCGG 1 cut(s) 137
Hin1II CATG 1 cut(s) 214
HinfI GANTC 2 cut(s) 74, 199
HpaII CCGG 1 cut(s) 137
HphI GGTGA 1 cut(s) 161
Hpy188I TCNGA 1 cut(s) 22
Hpy188III TCNNGA 1 cut(s) 203
HpyCH4V TGCA 1 cut(s) 8
HpyF10VI GCNNNNNNNGC 1 cut(s) 14
Hsp92II CATG 1 cut(s) 214
Kzo9I GATC 1 cut(s) 156
LpnPI CCDG 2 cut(s) 150, 188
Lsp1109I GCAGC 1 cut(s) 37
LweI GCATC 1 cut(s) 25
MalI GATC 1 cut(s) 158
MboI GATC 1 cut(s) 156
MhlI GDGCHC 1 cut(s) 128
MluCI AATT 2 cut(s) 57, 152
MlyI GAGTC 1 cut(s) 83
MnlI CCTC 1 cut(s) 28
MseI TTAA 2 cut(s) 96, 186
MspI CCGG 1 cut(s) 137
MwoI GCNNNNNNNGC 1 cut(s) 14
NdeII GATC 1 cut(s) 156
NlaIII CATG 1 cut(s) 214
NlaIV GGNNCC 1 cut(s) 127
PcsI WCGNNNNNNNCGW 1 cut(s) 69
PfeI GAWTC 1 cut(s) 199
PkrI GCNGC 1 cut(s) 52
PleI GAGTC 1 cut(s) 82
PpsI GAGTC 1 cut(s) 82
PspN4I GGNNCC 1 cut(s) 127
PspPI GGNCC 1 cut(s) 176
SaqAI TTAA 2 cut(s) 96, 186
SatI GCNGC 1 cut(s) 51
Sau3AI GATC 1 cut(s) 156
Sau96I GGNCC 1 cut(s) 176
SchI GAGTC 1 cut(s) 83
SduI GDGCHC 1 cut(s) 128
SetI ASST 1 cut(s) 198
SfaNI GCATC 1 cut(s) 25
SgeI CNNG 7 cut(s) 43, 83, 85, 135, 149, 192, 215
SinI GGWCC 1 cut(s) 176
SmlI CTYRAG 1 cut(s) 29
SmoI CTYRAG 1 cut(s) 29
Sse9I AATT 2 cut(s) 57, 152
SsiI CCGC 1 cut(s) 122
TaqII GACCGA 1 cut(s) 193
TasI AATT 2 cut(s) 57, 152
TfiI GAWTC 1 cut(s) 199
Tru1I TTAA 2 cut(s) 96, 186
Tru9I TTAA 2 cut(s) 96, 186
TseI GCWGC 1 cut(s) 50
TspDTI ATGAA 1 cut(s) 105
VpaK11BI GGWCC 1 cut(s) 176
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.