Rh4DG172800

Macrophage migration inhibitory factor homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0019403
Physical Location & Seq
Reverse (-)
34406772 .. 34417479
10708 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG172800.1

Sequence Viewer

Length: 369 bp
ATGGATGCAGTAATAGCGTCTGATATTCTCAAGGATGCCACCAAAGTCATTGCCCAAATTATCGGCAAACCCGAGTCCTATGTGATGATTTTGCTCAATGGGATTGTGCCAATTTCATTTGCGGGCACCAAAGAGCCGGTTGTGTATGGGGAATTGATCTCCATTTGGGGCATTGGACCGAGTGTTAATGGGAAGCTGATTCTGGAGACCATGCCAAGCAACCGTGCTGCTGCGTTTCTGCAATTGGTTCAGCTTAACAAAGCCCTCGCTGATGCAGAAACAACAATTACATTGAACCCCAAGTGGGAAAAGGTATATTTCAGGAAACGATGCATATTAAGGCCATGGAGCAATATGATGATGCTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00350 ko00360 map00350 map00360
Pfam Domains
Protein Families
MIF

Protein Analysis

122

Amino Acids

13.5

Weight (kDa)

9.13

Isoelectric Point (pI)

26.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MIF PF01187 4 - 64 3.6e-08 Macrophage migration inhibitory factor (MIF)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 125
AciI CCGC 1 cut(s) 122
AfiI CCNNNNNNNGG 1 cut(s) 304
AgsI TTSAA 1 cut(s) 295
AluBI AGCT 2 cut(s) 196, 253
AluI AGCT 2 cut(s) 196, 253
Alw26I GTCTC 1 cut(s) 200
Ama87I CYCGRG 1 cut(s) 71
AoxI GGCC 1 cut(s) 341
ApeKI GCWGC 2 cut(s) 227, 230
AspS9I GGNCC 1 cut(s) 176
AvaI CYCGRG 1 cut(s) 71
AvaII GGWCC 1 cut(s) 176
BaeGI GKGCMC 1 cut(s) 128
BanI GGYRCC 1 cut(s) 125
BbvI GCAGC 2 cut(s) 214, 217
BcoDI GTCTC 1 cut(s) 200
BisI GCNGC 2 cut(s) 228, 231
BlsI GCNGC 2 cut(s) 229, 232
Bme18I GGWCC 1 cut(s) 176
BmeT110I CYCGRG 1 cut(s) 71
BmgT120I GGNCC 1 cut(s) 176
BmiI GGNNCC 1 cut(s) 127
BmsI GCATC 4 cut(s) 25, 262, 320, 351
BpmI CTGGAG 1 cut(s) 224
BpuEI CTTGAG 1 cut(s) 14
BsaI GGTCTC 1 cut(s) 200
BsaJI CCNNGG 1 cut(s) 344
Bsc4I CCNNNNNNNGG 1 cut(s) 304
Bse118I RCCGGY 1 cut(s) 136
Bse3DI GCAATG 1 cut(s) 48
BseDI CCNNGG 1 cut(s) 344
BseGI GGATG 2 cut(s) 10, 40
BseLI CCNNNNNNNGG 1 cut(s) 304
BseMI GCAATG 1 cut(s) 48
BseSI GKGCMC 1 cut(s) 128
BseXI GCAGC 2 cut(s) 214, 217
BshFI GGCC 1 cut(s) 343
BshNI GGYRCC 1 cut(s) 125
BsiHKCI CYCGRG 1 cut(s) 71
BsiSI CCGG 1 cut(s) 137
BslI CCNNNNNNNGG 1 cut(s) 304
BsmAI GTCTC 1 cut(s) 200
BsnI GGCC 1 cut(s) 343
Bso31I GGTCTC 1 cut(s) 200
BsoBI CYCGRG 1 cut(s) 71
Bsp1286I GDGCHC 1 cut(s) 128
Bsp143I GATC 1 cut(s) 156
Bsp19I CCATGG 1 cut(s) 344
BspACI CCGC 1 cut(s) 122
BspANI GGCC 1 cut(s) 343
BspLI GGNNCC 1 cut(s) 127
BspT107I GGYRCC 1 cut(s) 125
BspTNI GGTCTC 1 cut(s) 200
BsrDI GCAATG 1 cut(s) 48
BsrFI RCCGGY 1 cut(s) 136
BssAI RCCGGY 1 cut(s) 136
BssECI CCNNGG 1 cut(s) 344
BssMI GATC 1 cut(s) 156
BssT1I CCWWGG 1 cut(s) 344
Bst4CI ACNGT 1 cut(s) 224
BstC8I GCNNGC 1 cut(s) 124
BstDSI CCRYGG 1 cut(s) 344
BstF5I GGATG 2 cut(s) 10, 40
BstKTI GATC 1 cut(s) 159
BstMAI GTCTC 1 cut(s) 200
BstMBI GATC 1 cut(s) 156
BstMWI GCNNNNNNNGC 1 cut(s) 14
BstSLI GKGCMC 1 cut(s) 128
BstV1I GCAGC 2 cut(s) 214, 217
BsuRI GGCC 1 cut(s) 343
BtgI CCRYGG 1 cut(s) 344
BtsCI GGATG 2 cut(s) 10, 40
Cac8I GCNNGC 1 cut(s) 124
Cfr10I RCCGGY 1 cut(s) 136
Cfr13I GGNCC 1 cut(s) 176
CseI GACGC 1 cut(s) 6
CviAII CATG 2 cut(s) 211, 345
CviJI RGCY 5 cut(s) 136, 196, 253, 263, 343
CviKI_1 RGCY 5 cut(s) 136, 196, 253, 263, 343
DpnI GATC 1 cut(s) 158
DpnII GATC 1 cut(s) 156
Eco130I CCWWGG 1 cut(s) 344
Eco31I GGTCTC 1 cut(s) 200
Eco47I GGWCC 1 cut(s) 176
Eco88I CYCGRG 1 cut(s) 71
EcoT14I CCWWGG 1 cut(s) 344
EcoT22I ATGCAT 1 cut(s) 335
ErhI CCWWGG 1 cut(s) 344
FaeI CATG 2 cut(s) 214, 348
FaiI YATR 7 cut(s) 81, 147, 212, 316, 335, 346, 356
FatI CATG 2 cut(s) 210, 344
FauI CCCGC 1 cut(s) 115
Fnu4HI GCNGC 2 cut(s) 228, 231
FokI GGATG 2 cut(s) 17, 47
Fsp4HI GCNGC 2 cut(s) 228, 231
GluI GCNGC 2 cut(s) 228, 231
GsuI CTGGAG 1 cut(s) 224
HaeIII GGCC 1 cut(s) 343
HapII CCGG 1 cut(s) 137
HgaI GACGC 1 cut(s) 6
Hin1II CATG 2 cut(s) 214, 348
HinfI GANTC 2 cut(s) 74, 199
HpaII CCGG 1 cut(s) 137
Hpy188I TCNGA 2 cut(s) 22, 368
Hpy188III TCNNGA 2 cut(s) 203, 322
HpyCH4III ACNGT 1 cut(s) 224
HpyCH4V TGCA 4 cut(s) 8, 241, 275, 333
HpyF10VI GCNNNNNNNGC 1 cut(s) 14
Hsp92II CATG 2 cut(s) 214, 348
Kzo9I GATC 1 cut(s) 156
LmnI GCTCC 1 cut(s) 348
LpnPI CCDG 3 cut(s) 150, 188, 307
Lsp1109I GCAGC 2 cut(s) 214, 217
LweI GCATC 4 cut(s) 25, 262, 320, 351
MalI GATC 1 cut(s) 158
MboI GATC 1 cut(s) 156
MfeI CAATTG 1 cut(s) 242
MhlI GDGCHC 1 cut(s) 128
MluCI AATT 5 cut(s) 57, 111, 152, 242, 285
MlyI GAGTC 1 cut(s) 83
MnlI CCTC 1 cut(s) 275
Mph1103I ATGCAT 1 cut(s) 335
MseI TTAA 3 cut(s) 186, 255, 338
MspI CCGG 1 cut(s) 137
MunI CAATTG 1 cut(s) 242
MwoI GCNNNNNNNGC 1 cut(s) 14
NcoI CCATGG 1 cut(s) 344
NdeII GATC 1 cut(s) 156
NlaIII CATG 2 cut(s) 214, 348
NlaIV GGNNCC 1 cut(s) 127
NsiI ATGCAT 1 cut(s) 335
PcsI WCGNNNNNNNCGW 1 cut(s) 69
PfeI GAWTC 1 cut(s) 199
PkrI GCNGC 2 cut(s) 229, 232
PleI GAGTC 1 cut(s) 82
PpsI GAGTC 1 cut(s) 82
PspN4I GGNNCC 1 cut(s) 127
PspPI GGNCC 1 cut(s) 176
SaqAI TTAA 3 cut(s) 186, 255, 338
SatI GCNGC 2 cut(s) 228, 231
Sau3AI GATC 1 cut(s) 156
Sau96I GGNCC 1 cut(s) 176
SchI GAGTC 1 cut(s) 83
SduI GDGCHC 1 cut(s) 128
SetI ASST 3 cut(s) 198, 255, 315
SfaNI GCATC 4 cut(s) 25, 262, 320, 351
SinI GGWCC 1 cut(s) 176
SmlI CTYRAG 1 cut(s) 29
SmoI CTYRAG 1 cut(s) 29
Sse9I AATT 5 cut(s) 57, 111, 152, 242, 285
SsiI CCGC 1 cut(s) 122
StyI CCWWGG 1 cut(s) 344
TaaI ACNGT 1 cut(s) 224
TaqII GACCGA 1 cut(s) 193
TasI AATT 5 cut(s) 57, 111, 152, 242, 285
TfiI GAWTC 1 cut(s) 199
Tru1I TTAA 3 cut(s) 186, 255, 338
Tru9I TTAA 3 cut(s) 186, 255, 338
TseI GCWGC 2 cut(s) 227, 230
TspDTI ATGAA 1 cut(s) 105
VpaK11BI GGWCC 1 cut(s) 176
Zsp2I ATGCAT 1 cut(s) 335
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.