Rh4BG109200

Belongs to the ubiquitin-conjugating enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0018711
Physical Location & Seq
Reverse (-)
18514876 .. 18525065
10190 bp
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UTR
Exon/CDS
Intron
Rh4BG109200.1

Sequence Viewer

Length: 444 bp
ATGTCGAGGTTATTACTGGCCAGACATGAAGAAACAAGCGGCAGAGACACAACTTTCATGTCCCAAATGCTCCTCGATCCCATCATAGCAGGAGGGACTTACAGCAGTAATCACAGAAGATTGGAGATCACCATACTTGGTTCTATGGGTAAGGACATGTTTCATTGGCAAGCAACGATTATGGGATCCCCTGATAGCCCATATGCTGGAGGTGTATTCCTTGTCACCATTCATTCCCCTCCTGACTATCCTTTCAAACCACCTAAGGTTGCATTTAGGACTAAGTGCTTACTATCTCTAAGAGGAGGAGGTTGGAAGGTGAAAGATGAGGGAGAGAGATCAAGAGGTTGCGGCAGAGGAAGGGGCTGTCGTGGTGGTGGCGGCGGCTCTGGCAAAGCTAGAGTTGATGCACTCAGTATATTATTCCACAACCTTGAATTGTAG

Protein Analysis

147

Amino Acids

15.99

Weight (kDa)

9.72

Isoelectric Point (pI)

53.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UQ_con PF00179 50 - 96 2.8e-14 Ubiquitin-conjugating enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0018711)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr5g0047921
rosa_multiflora Rmu_sc0010407.1_g000017
rosa_rugosa Rorug03G0288700 Rorug03G0290500 Rorug03G0290500
rosa_samantha Rh4AG101600 Rh4AG203400 Rh4BG109200 Rh4CG109600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 206
AciI CCGC 4 cut(s) 39, 351, 381, 384
AclWI GGATC 3 cut(s) 71, 180, 193
AcoI YGGCCR 1 cut(s) 18
AfiI CCNNNNNNNGG 1 cut(s) 206
AflIII ACRYGT 1 cut(s) 156
AgsI TTSAA 2 cut(s) 256, 437
AluBI AGCT 1 cut(s) 398
AluI AGCT 1 cut(s) 398
Alw26I GTCTC 1 cut(s) 39
AlwI GGATC 3 cut(s) 71, 180, 193
AoxI GGCC 1 cut(s) 18
AsuHPI GGTGA 3 cut(s) 121, 217, 331
AxyI CCTNAGG 1 cut(s) 264
BalI TGGCCA 1 cut(s) 20
BamHI GGATCC 1 cut(s) 185
BccI CCATC 1 cut(s) 89
BcoDI GTCTC 1 cut(s) 39
BfaI CTAG 1 cut(s) 399
BisI GCNGC 4 cut(s) 40, 352, 382, 385
BlsI GCNGC 4 cut(s) 41, 353, 383, 386
BmiI GGNNCC 1 cut(s) 187
BmsI GCATC 1 cut(s) 397
BpmI CTGGAG 1 cut(s) 228
Bsc4I CCNNNNNNNGG 1 cut(s) 206
Bse1I ACTGG 1 cut(s) 21
Bse21I CCTNAGG 1 cut(s) 264
BseLI CCNNNNNNNGG 1 cut(s) 206
BseMII CTCAG 1 cut(s) 427
BseNI ACTGG 1 cut(s) 21
BseRI GAGGAG 3 cut(s) 62, 318, 321
BshFI GGCC 1 cut(s) 20
BslFI GGGAC 2 cut(s) 46, 109
BslI CCNNNNNNNGG 1 cut(s) 206
BsmAI GTCTC 1 cut(s) 39
BsmFI GGGAC 2 cut(s) 46, 109
BsnI GGCC 1 cut(s) 20
Bsp143I GATC 4 cut(s) 76, 126, 185, 338
BspACI CCGC 4 cut(s) 39, 351, 381, 384
BspANI GGCC 1 cut(s) 20
BspCNI CTCAG 1 cut(s) 426
BspLI GGNNCC 1 cut(s) 187
BspPI GGATC 3 cut(s) 71, 180, 193
BsrI ACTGG 1 cut(s) 21
BssMI GATC 4 cut(s) 76, 126, 185, 338
BstC8I GCNNGC 1 cut(s) 171
BstDEI CTNAG 4 cut(s) 264, 282, 299, 413
BstKTI GATC 4 cut(s) 79, 129, 188, 341
BstMAI GTCTC 1 cut(s) 39
BstMBI GATC 4 cut(s) 76, 126, 185, 338
BstMWI GCNNNNNNNGC 1 cut(s) 390
BstNSI RCATGY 1 cut(s) 160
BstX2I RGATCY 1 cut(s) 185
BstYI RGATCY 1 cut(s) 185
Bsu36I CCTNAGG 1 cut(s) 264
BsuRI GGCC 1 cut(s) 20
Cac8I GCNNGC 1 cut(s) 171
CviAII CATG 3 cut(s) 26, 58, 157
CviJI RGCY 5 cut(s) 20, 198, 366, 387, 398
CviKI_1 RGCY 5 cut(s) 20, 198, 366, 387, 398
DdeI CTNAG 4 cut(s) 264, 282, 299, 413
DpnI GATC 4 cut(s) 78, 128, 187, 340
DpnII GATC 4 cut(s) 76, 126, 185, 338
EaeI YGGCCR 1 cut(s) 18
Eco81I CCTNAGG 1 cut(s) 264
FaeI CATG 3 cut(s) 29, 61, 160
FaqI GGGAC 2 cut(s) 46, 109
FatI CATG 3 cut(s) 25, 57, 156
FauNDI CATATG 1 cut(s) 202
Fnu4HI GCNGC 4 cut(s) 40, 352, 382, 385
Fsp4HI GCNGC 4 cut(s) 40, 352, 382, 385
FspBI CTAG 1 cut(s) 399
GluI GCNGC 4 cut(s) 40, 352, 382, 385
GsuI CTGGAG 1 cut(s) 228
HaeIII GGCC 1 cut(s) 20
Hin1II CATG 3 cut(s) 29, 61, 160
HphI GGTGA 3 cut(s) 121, 217, 331
Hpy188III TCNNGA 2 cut(s) 242, 342
HpyAV CCTTC 2 cut(s) 310, 354
HpyCH4V TGCA 2 cut(s) 272, 410
HpyF10VI GCNNNNNNNGC 1 cut(s) 390
HpyF3I CTNAG 4 cut(s) 264, 282, 299, 413
Hsp92II CATG 3 cut(s) 29, 61, 160
Kzo9I GATC 4 cut(s) 76, 126, 185, 338
LmnI GCTCC 1 cut(s) 75
LpnPI CCDG 7 cut(s) 2, 34, 75, 192, 204, 255, 375
LweI GCATC 1 cut(s) 397
MaeI CTAG 1 cut(s) 399
MaeIII GTNAC 1 cut(s) 223
MalI GATC 4 cut(s) 78, 128, 187, 340
MboI GATC 4 cut(s) 76, 126, 185, 338
MboII GAAGA 2 cut(s) 41, 129
MflI RGATCY 1 cut(s) 185
MlsI TGGCCA 1 cut(s) 20
MluCI AATT 1 cut(s) 437
MluNI TGGCCA 1 cut(s) 20
MmeI TCCRAC 1 cut(s) 293
Mox20I TGGCCA 1 cut(s) 20
MscI TGGCCA 1 cut(s) 20
Msp20I TGGCCA 1 cut(s) 20
MwoI GCNNNNNNNGC 1 cut(s) 390
NdeI CATATG 1 cut(s) 202
NdeII GATC 4 cut(s) 76, 126, 185, 338
NlaIII CATG 3 cut(s) 29, 61, 160
NlaIV GGNNCC 1 cut(s) 187
NmuCI GTSAC 1 cut(s) 223
NspI RCATGY 1 cut(s) 160
PciI ACATGT 1 cut(s) 156
PflMI CCANNNNNTGG 1 cut(s) 206
PkrI GCNGC 4 cut(s) 41, 353, 383, 386
PscI ACATGT 1 cut(s) 156
PspN4I GGNNCC 1 cut(s) 187
PsuI RGATCY 1 cut(s) 185
SatI GCNGC 4 cut(s) 40, 352, 382, 385
Sau3AI GATC 4 cut(s) 76, 126, 185, 338
SetI ASST 9 cut(s) 11, 214, 265, 270, 313, 321, 349, 400, 435
SfaNI GCATC 1 cut(s) 397
Sse9I AATT 1 cut(s) 437
SsiI CCGC 4 cut(s) 39, 351, 381, 384
SspMI CTAG 1 cut(s) 399
TaqI TCGA 2 cut(s) 5, 75
TasI AATT 1 cut(s) 437
TauI GCSGC 4 cut(s) 42, 354, 384, 387
TseFI GTSAC 1 cut(s) 223
Tsp45I GTSAC 1 cut(s) 223
TspDTI ATGAA 4 cut(s) 42, 46, 152, 221
Van91I CCANNNNNTGG 1 cut(s) 206
XceI RCATGY 1 cut(s) 160
XspI CTAG 1 cut(s) 399
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.