Rh4CG010300

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0021523
Physical Location & Seq
Forward (+)
1817960 .. 1818430
471 bp
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UTR
Exon/CDS
Intron
Rh4CG010300.1

Sequence Viewer

Length: 471 bp
ATGTCCGGTGTTCAGGTGGTGGTTAGAGTGCAGCAGGGAGGGACGAAGGAAGGGAGCTCTTCCAATTCTACCCCTAACCCTAACCCTAGTGGGTCTTTGGTGTGCGGCGAACTCGAGTCTGGTTCGAAGGGCGTGCCTTTGCGTCCGTGTGTGGGCTGCAGTGCAGCAGTGCCGCTACCAACGATCATCAATCCTTTTGCTCCCTCTGGCTTTGTGCTTTTTGTCCCAGGTGTTTCGGCAAAAGAGTTAACTAGGCAGTTAGCACATTATCGTCCTAAGGAAGTTCTCTTGGTGATTCCTCCTCCTCGCCGACGTCTTGTTGAGGAGCATGTGGTGGCTAGTGGAATGAAGTGGTCTGTAGATACCATGGAACTGTTAACATACAATAAGAAGGCTAAGGTTGTAGTGCCTGTTCCCAAAACTACTTTTGTGGACTTAGGGTTTGTGCTGGAATTAGCGATCCCTCAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

156

Amino Acids

16.61

Weight (kDa)

9.33

Isoelectric Point (pI)

44.75

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0021523)

Species Orthologous Gene IDs
rosa_laevigata RLG00000010187
rosa_multiflora Rmu_sc0003264.1_g000030
rosa_samantha Rh4AG009700 Rh4CG010300
rosa_wichuraiana Rw4G000170

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 316
AciI CCGC 2 cut(s) 105, 173
AclWI GGATC 1 cut(s) 454
AcyI GRCGYC 1 cut(s) 313
AfiI CCNNNNNNNGG 1 cut(s) 152
AjnI CCWGG 1 cut(s) 226
AluBI AGCT 1 cut(s) 57
AluI AGCT 1 cut(s) 57
Alw21I GWGCWC 1 cut(s) 59
AlwI GGATC 1 cut(s) 454
Ama87I CYCGRG 1 cut(s) 113
ApeKI GCWGC 3 cut(s) 31, 156, 164
ArsI GACNNNNNNTTYG 2 cut(s) 425, 457
AsuHPI GGTGA 1 cut(s) 304
AsuII TTCGAA 1 cut(s) 125
AvaI CYCGRG 1 cut(s) 113
AxyI CCTNAGG 1 cut(s) 276
BanII GRGCYC 1 cut(s) 59
Bbv12I GWGCWC 1 cut(s) 59
BbvI GCAGC 3 cut(s) 43, 143, 176
BcgI CGANNNNNNTGC 2 cut(s) 115, 149
BciT130I CCWGG 1 cut(s) 228
BfaI CTAG 3 cut(s) 87, 252, 339
BfmI CTRYAG 2 cut(s) 157, 357
BisI GCNGC 5 cut(s) 32, 106, 157, 165, 173
BlsI GCNGC 5 cut(s) 33, 107, 158, 166, 174
Bme1390I CCNGG 1 cut(s) 228
BmeT110I CYCGRG 1 cut(s) 113
BmrFI CCNGG 1 cut(s) 228
Bpu10I CCTNAGC 1 cut(s) 396
Bpu14I TTCGAA 1 cut(s) 125
BsaHI GRCGYC 1 cut(s) 313
BsaJI CCNNGG 2 cut(s) 226, 366
BsaWI WCCGGW 1 cut(s) 5
Bsc4I CCNNNNNNNGG 1 cut(s) 152
Bse21I CCTNAGG 1 cut(s) 276
BseBI CCWGG 1 cut(s) 228
BseDI CCNNGG 2 cut(s) 226, 366
BseLI CCNNNNNNNGG 1 cut(s) 152
BseRI GAGGAG 3 cut(s) 291, 294, 338
BseXI GCAGC 3 cut(s) 43, 143, 176
BsgI GTGCAG 2 cut(s) 50, 183
BsiHKAI GWGCWC 1 cut(s) 59
BsiHKCI CYCGRG 1 cut(s) 113
BsiSI CCGG 1 cut(s) 6
BslFI GGGAC 2 cut(s) 55, 209
BslI CCNNNNNNNGG 1 cut(s) 152
BsmFI GGGAC 2 cut(s) 55, 209
BsoBI CYCGRG 1 cut(s) 113
Bsp119I TTCGAA 1 cut(s) 125
Bsp1286I GDGCHC 1 cut(s) 59
Bsp143I GATC 2 cut(s) 183, 459
Bsp19I CCATGG 1 cut(s) 366
BspACI CCGC 2 cut(s) 105, 173
BspMAI CTGCAG 1 cut(s) 161
BspPI GGATC 1 cut(s) 454
BspQI GCTCTTC 1 cut(s) 64
BspT104I TTCGAA 1 cut(s) 125
BssECI CCNNGG 2 cut(s) 226, 366
BssMI GATC 2 cut(s) 183, 459
BssNI GRCGYC 1 cut(s) 313
BssT1I CCWWGG 1 cut(s) 366
Bst2UI CCWGG 1 cut(s) 228
Bst4CI ACNGT 1 cut(s) 375
Bst6I CTCTTC 1 cut(s) 64
BstACI GRCGYC 1 cut(s) 313
BstBI TTCGAA 1 cut(s) 125
BstC8I GCNNGC 1 cut(s) 134
BstDEI CTNAG 3 cut(s) 276, 396, 436
BstDSI CCRYGG 1 cut(s) 366
BstKTI GATC 2 cut(s) 186, 462
BstMBI GATC 2 cut(s) 183, 459
BstNI CCWGG 1 cut(s) 228
BstNSI RCATGY 1 cut(s) 332
BstSCI CCNGG 1 cut(s) 226
BstSFI CTRYAG 2 cut(s) 157, 357
BstV1I GCAGC 3 cut(s) 43, 143, 176
Bsu36I CCTNAGG 1 cut(s) 276
BtgI CCRYGG 1 cut(s) 366
BtsI GCAGTG 2 cut(s) 166, 174
BtsIMutI CAGTG 2 cut(s) 166, 174
Cac8I GCNNGC 1 cut(s) 134
CseI GACGC 1 cut(s) 131
CviAII CATG 2 cut(s) 329, 367
CviJI RGCY 5 cut(s) 57, 156, 210, 338, 395
CviKI_1 RGCY 5 cut(s) 57, 156, 210, 338, 395
DdeI CTNAG 3 cut(s) 276, 396, 436
DpnI GATC 2 cut(s) 185, 461
DpnII GATC 2 cut(s) 183, 459
Eam1104I CTCTTC 1 cut(s) 64
EarI CTCTTC 1 cut(s) 64
Ecl136II GAGCTC 1 cut(s) 57
Eco130I CCWWGG 1 cut(s) 366
Eco24I GRGCYC 1 cut(s) 59
Eco53kI GAGCTC 1 cut(s) 57
Eco81I CCTNAGG 1 cut(s) 276
Eco88I CYCGRG 1 cut(s) 113
EcoICRI GAGCTC 1 cut(s) 57
EcoRII CCWGG 1 cut(s) 226
EcoT14I CCWWGG 1 cut(s) 366
EcoT38I GRGCYC 1 cut(s) 59
ErhI CCWWGG 1 cut(s) 366
FaeI CATG 2 cut(s) 332, 370
FaiI YATR 3 cut(s) 330, 368, 382
FaqI GGGAC 2 cut(s) 55, 209
FatI CATG 2 cut(s) 328, 366
Fnu4HI GCNGC 5 cut(s) 32, 106, 157, 165, 173
FriOI GRGCYC 1 cut(s) 59
Fsp4HI GCNGC 5 cut(s) 32, 106, 157, 165, 173
FspBI CTAG 3 cut(s) 87, 252, 339
GluI GCNGC 5 cut(s) 32, 106, 157, 165, 173
HapII CCGG 1 cut(s) 6
HgaI GACGC 1 cut(s) 131
Hin1I GRCGYC 1 cut(s) 313
Hin1II CATG 2 cut(s) 332, 370
HincII GTYRAC 2 cut(s) 249, 378
HindII GTYRAC 2 cut(s) 249, 378
HinfI GANTC 2 cut(s) 116, 295
HpaI GTTAAC 2 cut(s) 249, 378
HpaII CCGG 1 cut(s) 6
HphI GGTGA 1 cut(s) 304
Hpy166II GTNNAC 3 cut(s) 249, 378, 433
Hpy8I GTNNAC 3 cut(s) 249, 378, 433
Hpy99I CGWCG 1 cut(s) 315
HpyAV CCTTC 4 cut(s) 40, 44, 121, 385
HpyCH4III ACNGT 1 cut(s) 375
HpyCH4IV ACGT 1 cut(s) 313
HpyCH4V TGCA 3 cut(s) 31, 159, 164
HpyF3I CTNAG 3 cut(s) 276, 396, 436
HpySE526I ACGT 1 cut(s) 313
Hsp92I GRCGYC 1 cut(s) 313
Hsp92II CATG 2 cut(s) 332, 370
KspAI GTTAAC 2 cut(s) 249, 378
Kzo9I GATC 2 cut(s) 183, 459
LguI GCTCTTC 1 cut(s) 64
LmnI GCTCC 3 cut(s) 54, 205, 325
LpnPI CCDG 8 cut(s) 19, 20, 105, 192, 213, 240, 423, 434
Lsp1109I GCAGC 3 cut(s) 43, 143, 176
MaeI CTAG 3 cut(s) 87, 252, 339
MaeII ACGT 1 cut(s) 313
MalI GATC 2 cut(s) 185, 461
MboI GATC 2 cut(s) 183, 459
MboII GAAGA 1 cut(s) 51
MhlI GDGCHC 1 cut(s) 59
MluCI AATT 2 cut(s) 64, 452
MlyI GAGTC 1 cut(s) 125
MnlI CCTC 6 cut(s) 32, 214, 309, 312, 315, 316
MseI TTAA 2 cut(s) 248, 377
MspI CCGG 1 cut(s) 6
MspR9I CCNGG 1 cut(s) 228
MvaI CCWGG 1 cut(s) 228
NcoI CCATGG 1 cut(s) 366
NdeII GATC 2 cut(s) 183, 459
NlaIII CATG 2 cut(s) 332, 370
NspI RCATGY 1 cut(s) 332
NspV TTCGAA 1 cut(s) 125
PaeR7I CTCGAG 1 cut(s) 113
PciSI GCTCTTC 1 cut(s) 64
PfeI GAWTC 1 cut(s) 295
PkrI GCNGC 5 cut(s) 33, 107, 158, 166, 174
PleI GAGTC 1 cut(s) 124
PpsI GAGTC 1 cut(s) 124
Psp124BI GAGCTC 1 cut(s) 59
Psp6I CCWGG 1 cut(s) 226
PspGI CCWGG 1 cut(s) 226
PspXI VCTCGAGB 1 cut(s) 113
PsrI GAACNNNNNNTAC 2 cut(s) 396, 428
PstI CTGCAG 1 cut(s) 161
SacI GAGCTC 1 cut(s) 59
SapI GCTCTTC 1 cut(s) 64
SaqAI TTAA 2 cut(s) 248, 377
SatI GCNGC 5 cut(s) 32, 106, 157, 165, 173
Sau3AI GATC 2 cut(s) 183, 459
SchI GAGTC 1 cut(s) 125
ScrFI CCNGG 1 cut(s) 228
SduI GDGCHC 1 cut(s) 59
SetI ASST 5 cut(s) 18, 59, 232, 316, 402
SfcI CTRYAG 2 cut(s) 157, 357
Sfr274I CTCGAG 1 cut(s) 113
SfuI TTCGAA 1 cut(s) 125
SlaI CTCGAG 1 cut(s) 113
SmlI CTYRAG 1 cut(s) 113
SmoI CTYRAG 1 cut(s) 113
Sse9I AATT 2 cut(s) 64, 452
SsiI CCGC 2 cut(s) 105, 173
SspMI CTAG 3 cut(s) 87, 252, 339
SstI GAGCTC 1 cut(s) 59
StyD4I CCNGG 1 cut(s) 226
StyI CCWWGG 1 cut(s) 366
TaaI ACNGT 1 cut(s) 375
TaiI ACGT 1 cut(s) 316
TaqI TCGA 2 cut(s) 114, 125
TasI AATT 2 cut(s) 64, 452
TauI GCSGC 2 cut(s) 108, 175
TfiI GAWTC 1 cut(s) 295
Tru1I TTAA 2 cut(s) 248, 377
Tru9I TTAA 2 cut(s) 248, 377
TscAI CASTG 2 cut(s) 166, 174
TseI GCWGC 3 cut(s) 31, 156, 164
TspDTI ATGAA 1 cut(s) 362
TspGWI ACGGA 1 cut(s) 135
TspRI CASTG 2 cut(s) 166, 174
XceI RCATGY 1 cut(s) 332
XhoI CTCGAG 1 cut(s) 113
XspI CTAG 3 cut(s) 87, 252, 339
ZraI GACGTC 1 cut(s) 314
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.