Rh5AG216800

Belongs to the ubiquitin-conjugating enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0019135
Physical Location & Seq
Forward (+)
25545202 .. 25549485
4284 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG216800.1

Sequence Viewer

Length: 363 bp
ATGCGGTATTTCAATGTGATGATCCTTGGCCCGACTCAATCTCCGTATGAAGGAGGAGTTTTCAAGTTGGAACTATTTTTGCCTGAAGAATATCCTATGGCTGCACCAAAGGTTCGGTTTCTCACTAAAATTTATCATCCTAACATTGACAAGCTGGGAAGGATATGCCTTGACATTTTGAAAGACAAGTGGAGTCCTGCTCTACAGATACGAACTGTTCTTCTCAGTATTCAAGCACTTTTGAGTGCTCCAAATCCCGATGATCCGCTCTCTGAGAACATCGCTAAGCATTGGAAATCAAATGAGGCGGAAGCTGTTGAAACAGCCAAGGAATGGACCCGTTTATATGCAAGTGGTGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04120 ko04624 map04120 map04624
Pfam Domains
Protein Families
UQ_con

Protein Analysis

120

Amino Acids

13.66

Weight (kDa)

6.73

Isoelectric Point (pI)

40.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UQ_con PF00179 2 - 112 2.7e-45 Ubiquitin-conjugating enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes (Group: OG0019135)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G16890 AT1G78870 AT1G78870 AT1G78870
rosa_multiflora Rmu_sc0006236.1_g000001
rosa_samantha Rh5AG216800 Rh5BG216300 Rh5DG221400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 333
AccBSI CCGCTC 1 cut(s) 268
AciI CCGC 3 cut(s) 4, 266, 308
AclWI GGATC 2 cut(s) 16, 257
AcsI RAATTY 1 cut(s) 129
AcuI CTGAAG 1 cut(s) 105
AfiI CCNNNNNNNGG 2 cut(s) 50, 333
AgsI TTSAA 5 cut(s) 13, 64, 181, 233, 320
AluBI AGCT 2 cut(s) 154, 314
AluI AGCT 2 cut(s) 154, 314
Alw21I GWGCWC 1 cut(s) 250
AlwI GGATC 2 cut(s) 16, 257
AoxI GGCC 1 cut(s) 28
ApeKI GCWGC 1 cut(s) 101
ApoI RAATTY 1 cut(s) 129
AspS9I GGNCC 2 cut(s) 29, 336
AvaII GGWCC 1 cut(s) 336
Bbv12I GWGCWC 1 cut(s) 250
BbvI GCAGC 1 cut(s) 88
BfmI CTRYAG 1 cut(s) 203
BisI GCNGC 1 cut(s) 102
BlpI GCTNAGC 1 cut(s) 285
BlsI GCNGC 1 cut(s) 103
Bme18I GGWCC 1 cut(s) 336
BmgT120I GGNCC 2 cut(s) 29, 336
BmiI GGNNCC 1 cut(s) 338
BplI GAGNNNNNCTC 2 cut(s) 184, 216
Bpu1102I GCTNAGC 1 cut(s) 285
BsaJI CCNNGG 2 cut(s) 25, 327
BsaXI ACNNNNNCTCC 2 cut(s) 25, 55
Bsc4I CCNNNNNNNGG 2 cut(s) 50, 333
BseDI CCNNGG 2 cut(s) 25, 327
BseGI GGATG 1 cut(s) 136
BseLI CCNNNNNNNGG 2 cut(s) 50, 333
BseMII CTCAG 2 cut(s) 238, 264
BseRI GAGGAG 1 cut(s) 69
BseXI GCAGC 1 cut(s) 88
BseYI CCCAGC 1 cut(s) 154
BsgI GTGCAG 1 cut(s) 87
BshFI GGCC 1 cut(s) 30
BsiHKAI GWGCWC 1 cut(s) 250
BslI CCNNNNNNNGG 2 cut(s) 50, 333
BsnI GGCC 1 cut(s) 30
Bsp1286I GDGCHC 1 cut(s) 250
Bsp143I GATC 2 cut(s) 21, 262
Bsp1720I GCTNAGC 1 cut(s) 285
BspACI CCGC 3 cut(s) 4, 266, 308
BspANI GGCC 1 cut(s) 30
BspCNI CTCAG 2 cut(s) 237, 265
BspLI GGNNCC 1 cut(s) 338
BspPI GGATC 2 cut(s) 16, 257
BsrBI CCGCTC 1 cut(s) 268
BssECI CCNNGG 2 cut(s) 25, 327
BssMI GATC 2 cut(s) 21, 262
BssT1I CCWWGG 2 cut(s) 25, 327
Bst4CI ACNGT 1 cut(s) 217
BstDEI CTNAG 3 cut(s) 224, 273, 285
BstF5I GGATG 1 cut(s) 136
BstKTI GATC 2 cut(s) 24, 265
BstMBI GATC 2 cut(s) 21, 262
BstSFI CTRYAG 1 cut(s) 203
BstV1I GCAGC 1 cut(s) 88
BsuRI GGCC 1 cut(s) 30
BtgZI GCGATG 1 cut(s) 265
BtsCI GGATG 1 cut(s) 136
Cfr13I GGNCC 2 cut(s) 29, 336
CviJI RGCY 5 cut(s) 30, 101, 154, 314, 326
CviKI_1 RGCY 5 cut(s) 30, 101, 154, 314, 326
DdeI CTNAG 3 cut(s) 224, 273, 285
DpnI GATC 2 cut(s) 23, 264
DpnII GATC 2 cut(s) 21, 262
EciI GGCGGA 1 cut(s) 323
Eco130I CCWWGG 2 cut(s) 25, 327
Eco47I GGWCC 1 cut(s) 336
Eco57I CTGAAG 1 cut(s) 105
EcoT14I CCWWGG 2 cut(s) 25, 327
ErhI CCWWGG 2 cut(s) 25, 327
FaiI YATR 5 cut(s) 48, 98, 166, 346, 348
Fnu4HI GCNGC 1 cut(s) 102
FokI GGATG 1 cut(s) 123
Fsp4HI GCNGC 1 cut(s) 102
GluI GCNGC 1 cut(s) 102
GsaI CCCAGC 1 cut(s) 158
HaeIII GGCC 1 cut(s) 30
HinfI GANTC 2 cut(s) 34, 193
Hpy188I TCNGA 1 cut(s) 274
Hpy188III TCNNGA 1 cut(s) 257
HpyAV CCTTC 2 cut(s) 44, 153
HpyCH4III ACNGT 1 cut(s) 217
HpyCH4V TGCA 2 cut(s) 104, 350
HpyF3I CTNAG 3 cut(s) 224, 273, 285
Kzo9I GATC 2 cut(s) 21, 262
LmnI GCTCC 1 cut(s) 253
LpnPI CCDG 3 cut(s) 96, 140, 210
Lsp1109I GCAGC 1 cut(s) 88
MalI GATC 2 cut(s) 23, 264
MbiI CCGCTC 1 cut(s) 268
MboI GATC 2 cut(s) 21, 262
MboII GAAGA 2 cut(s) 98, 212
MhlI GDGCHC 1 cut(s) 250
MluCI AATT 1 cut(s) 129
MlyI GAGTC 2 cut(s) 28, 202
MmeI TCCRAC 1 cut(s) 48
MnlI CCTC 2 cut(s) 47, 298
NdeII GATC 2 cut(s) 21, 262
NlaIV GGNNCC 1 cut(s) 338
PflMI CCANNNNNTGG 1 cut(s) 333
PkrI GCNGC 1 cut(s) 103
PleI GAGTC 2 cut(s) 28, 201
PpsI GAGTC 2 cut(s) 28, 201
PspFI CCCAGC 1 cut(s) 154
PspN4I GGNNCC 1 cut(s) 338
PspPI GGNCC 2 cut(s) 29, 336
SatI GCNGC 1 cut(s) 102
Sau3AI GATC 2 cut(s) 21, 262
Sau96I GGNCC 2 cut(s) 29, 336
SchI GAGTC 2 cut(s) 28, 202
SduI GDGCHC 1 cut(s) 250
SetI ASST 3 cut(s) 114, 156, 316
SfcI CTRYAG 1 cut(s) 203
SinI GGWCC 1 cut(s) 336
Sse9I AATT 1 cut(s) 129
SsiI CCGC 3 cut(s) 4, 266, 308
StyI CCWWGG 2 cut(s) 25, 327
TaaI ACNGT 1 cut(s) 217
TasI AATT 1 cut(s) 129
TseI GCWGC 1 cut(s) 101
TspDTI ATGAA 1 cut(s) 63
TspGWI ACGGA 1 cut(s) 33
Van91I CCANNNNNTGG 1 cut(s) 333
VpaK11BI GGWCC 1 cut(s) 336
XapI RAATTY 1 cut(s) 129
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.