Rh5DG221400

Belongs to the ubiquitin-conjugating enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0019135
Physical Location & Seq
Forward (+)
24883564 .. 24887848
4285 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG221400.1

Sequence Viewer

Length: 417 bp
ATGTGTTGTGATTTTACAGCTCCGGGAATTAGTGCTTCGCCATCGGAAGACAATATGCGGTATTTCAATGTGATGATCCTTGGCCCGACTCAATCTCCGTATGAAGGAGGAGTTTTCAAGTTGGAACTATTTTTGCCTGAAGAATATCCTATGGCTGCACCAAAGGTTCGGTTTCTCACTAAAATTTATCATCCTAACATTGACAAGCTGGGAAGGATATGCCTTGACATTTTGAAAGACAAGTGGAGTCCTGCTCTACAGATACGAACTGTTCTTCTCAGTATTCAAGCACTTTTGAGTGCTCCAAATCCCGATGATCCGCTCTCTGAGAACATCGCTAAGCATTGGAAATCAAATGAGGCGGAAGCTGTTGAAACAGCCAAGGAATGGACCCGTTTATATGCAAGTGGTGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04120 ko04624 map04120 map04624
Pfam Domains
Protein Families
UQ_con

Protein Analysis

138

Amino Acids

15.49

Weight (kDa)

5.37

Isoelectric Point (pI)

45.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UQ_con PF00179 7 - 130 2.2e-48 Ubiquitin-conjugating enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019135)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G16890 AT1G78870 AT1G78870 AT1G78870
rosa_multiflora Rmu_sc0006236.1_g000001
rosa_samantha Rh5AG216800 Rh5BG216300 Rh5DG221400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 387
AccBSI CCGCTC 1 cut(s) 322
AciI CCGC 3 cut(s) 58, 320, 362
AclWI GGATC 2 cut(s) 70, 311
AcsI RAATTY 1 cut(s) 183
AcuI CTGAAG 1 cut(s) 159
AfiI CCNNNNNNNGG 2 cut(s) 104, 387
AgsI TTSAA 5 cut(s) 67, 118, 235, 287, 374
AluBI AGCT 3 cut(s) 20, 208, 368
AluI AGCT 3 cut(s) 20, 208, 368
Alw21I GWGCWC 1 cut(s) 304
AlwI GGATC 2 cut(s) 70, 311
AoxI GGCC 1 cut(s) 82
ApeKI GCWGC 1 cut(s) 155
ApoI RAATTY 1 cut(s) 183
AspS9I GGNCC 2 cut(s) 83, 390
AsuC2I CCSGG 1 cut(s) 24
AvaII GGWCC 1 cut(s) 390
BbsI GAAGAC 1 cut(s) 54
Bbv12I GWGCWC 1 cut(s) 304
BbvI GCAGC 1 cut(s) 142
BccI CCATC 1 cut(s) 49
BcnI CCSGG 1 cut(s) 24
BfmI CTRYAG 1 cut(s) 257
BisI GCNGC 1 cut(s) 156
BlpI GCTNAGC 1 cut(s) 339
BlsI GCNGC 1 cut(s) 157
Bme1390I CCNGG 1 cut(s) 24
Bme18I GGWCC 1 cut(s) 390
BmgT120I GGNCC 2 cut(s) 83, 390
BmiI GGNNCC 1 cut(s) 392
BmrFI CCNGG 1 cut(s) 24
BpiI GAAGAC 1 cut(s) 54
BplI GAGNNNNNCTC 2 cut(s) 238, 270
Bpu1102I GCTNAGC 1 cut(s) 339
BpuMI CCSGG 1 cut(s) 24
BsaJI CCNNGG 2 cut(s) 79, 381
BsaXI ACNNNNNCTCC 2 cut(s) 79, 109
Bsc4I CCNNNNNNNGG 2 cut(s) 104, 387
BseDI CCNNGG 2 cut(s) 79, 381
BseGI GGATG 1 cut(s) 190
BseLI CCNNNNNNNGG 2 cut(s) 104, 387
BseMII CTCAG 2 cut(s) 292, 318
BseRI GAGGAG 1 cut(s) 123
BseXI GCAGC 1 cut(s) 142
BseYI CCCAGC 1 cut(s) 208
BsgI GTGCAG 1 cut(s) 141
BshFI GGCC 1 cut(s) 84
BsiHKAI GWGCWC 1 cut(s) 304
BsiSI CCGG 1 cut(s) 23
BslI CCNNNNNNNGG 2 cut(s) 104, 387
BsnI GGCC 1 cut(s) 84
Bsp1286I GDGCHC 1 cut(s) 304
Bsp143I GATC 2 cut(s) 75, 316
Bsp1720I GCTNAGC 1 cut(s) 339
BspACI CCGC 3 cut(s) 58, 320, 362
BspANI GGCC 1 cut(s) 84
BspCNI CTCAG 2 cut(s) 291, 319
BspLI GGNNCC 1 cut(s) 392
BspPI GGATC 2 cut(s) 70, 311
BsrBI CCGCTC 1 cut(s) 322
BssECI CCNNGG 2 cut(s) 79, 381
BssMI GATC 2 cut(s) 75, 316
BssT1I CCWWGG 2 cut(s) 79, 381
Bst4CI ACNGT 1 cut(s) 271
BstDEI CTNAG 3 cut(s) 278, 327, 339
BstF5I GGATG 1 cut(s) 190
BstKTI GATC 2 cut(s) 78, 319
BstMBI GATC 2 cut(s) 75, 316
BstSCI CCNGG 1 cut(s) 22
BstSFI CTRYAG 1 cut(s) 257
BstV1I GCAGC 1 cut(s) 142
BstV2I GAAGAC 1 cut(s) 54
BsuRI GGCC 1 cut(s) 84
BtgZI GCGATG 1 cut(s) 319
BtsCI GGATG 1 cut(s) 190
Cfr13I GGNCC 2 cut(s) 83, 390
CviJI RGCY 6 cut(s) 20, 84, 155, 208, 368, 380
CviKI_1 RGCY 6 cut(s) 20, 84, 155, 208, 368, 380
DdeI CTNAG 3 cut(s) 278, 327, 339
DpnI GATC 2 cut(s) 77, 318
DpnII GATC 2 cut(s) 75, 316
EciI GGCGGA 1 cut(s) 377
Eco130I CCWWGG 2 cut(s) 79, 381
Eco47I GGWCC 1 cut(s) 390
Eco57I CTGAAG 1 cut(s) 159
EcoT14I CCWWGG 2 cut(s) 79, 381
ErhI CCWWGG 2 cut(s) 79, 381
FaiI YATR 6 cut(s) 56, 102, 152, 220, 400, 402
Fnu4HI GCNGC 1 cut(s) 156
FokI GGATG 1 cut(s) 177
Fsp4HI GCNGC 1 cut(s) 156
GluI GCNGC 1 cut(s) 156
GsaI CCCAGC 1 cut(s) 212
HaeIII GGCC 1 cut(s) 84
HapII CCGG 1 cut(s) 23
HinfI GANTC 2 cut(s) 88, 247
HpaII CCGG 1 cut(s) 23
Hpy188I TCNGA 2 cut(s) 46, 328
Hpy188III TCNNGA 1 cut(s) 311
HpyAV CCTTC 2 cut(s) 98, 207
HpyCH4III ACNGT 1 cut(s) 271
HpyCH4V TGCA 2 cut(s) 158, 404
HpyF3I CTNAG 3 cut(s) 278, 327, 339
Kzo9I GATC 2 cut(s) 75, 316
LmnI GCTCC 2 cut(s) 25, 307
LpnPI CCDG 4 cut(s) 36, 150, 194, 264
Lsp1109I GCAGC 1 cut(s) 142
MalI GATC 2 cut(s) 77, 318
MbiI CCGCTC 1 cut(s) 322
MboI GATC 2 cut(s) 75, 316
MboII GAAGA 3 cut(s) 59, 152, 266
MhlI GDGCHC 1 cut(s) 304
MluCI AATT 2 cut(s) 27, 183
MlyI GAGTC 2 cut(s) 82, 256
MmeI TCCRAC 1 cut(s) 102
MnlI CCTC 2 cut(s) 101, 352
MspI CCGG 1 cut(s) 23
MspR9I CCNGG 1 cut(s) 24
NciI CCSGG 1 cut(s) 24
NdeII GATC 2 cut(s) 75, 316
NlaIV GGNNCC 1 cut(s) 392
PflMI CCANNNNNTGG 1 cut(s) 387
PfoI TCCNGGA 1 cut(s) 22
PkrI GCNGC 1 cut(s) 157
PleI GAGTC 2 cut(s) 82, 255
PpsI GAGTC 2 cut(s) 82, 255
PspFI CCCAGC 1 cut(s) 208
PspN4I GGNNCC 1 cut(s) 392
PspPI GGNCC 2 cut(s) 83, 390
SatI GCNGC 1 cut(s) 156
Sau3AI GATC 2 cut(s) 75, 316
Sau96I GGNCC 2 cut(s) 83, 390
SchI GAGTC 2 cut(s) 82, 256
ScrFI CCNGG 1 cut(s) 24
SduI GDGCHC 1 cut(s) 304
SetI ASST 4 cut(s) 22, 168, 210, 370
SfcI CTRYAG 1 cut(s) 257
SinI GGWCC 1 cut(s) 390
Sse9I AATT 2 cut(s) 27, 183
SsiI CCGC 3 cut(s) 58, 320, 362
StyD4I CCNGG 1 cut(s) 22
StyI CCWWGG 2 cut(s) 79, 381
TaaI ACNGT 1 cut(s) 271
TasI AATT 2 cut(s) 27, 183
TseI GCWGC 1 cut(s) 155
TspDTI ATGAA 1 cut(s) 117
TspGWI ACGGA 1 cut(s) 87
Van91I CCANNNNNTGG 1 cut(s) 387
VpaK11BI GGWCC 1 cut(s) 390
XapI RAATTY 1 cut(s) 183
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.