Rh5AG329400

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0020664
Physical Location & Seq
Reverse (-)
50264294 .. 50282875
18582 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG329400.1

Sequence Viewer

Length: 258 bp
ATGATGGCATGGCATTATCTTATGAGCCTGTACATTTTGCAGTGTGATCCAGAAGAATACGGTTCATGTGACTCAGGCTATACTGGTGGACTAAGTCAAGGCTGGTGGCTTGGAGCAAGAGAAGGACTACCCTTAAAAATAGTGGGACTGATCGCGGTACCTGCAAATTTGACAAGACTAGCTCGTGGCACCTTGGCTACAATTGCGTATCAACTTTGTAAAAGCAAACGAATCCCAAGCAATGATAGTCAGGTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

85

Amino Acids

9.35

Weight (kDa)

7.71

Isoelectric Point (pI)

57.69

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0020664)

Species Orthologous Gene IDs
rosa_laevigata RLG00000017608
rosa_multiflora Rmu_sc0000147.1_g000061
rosa_roxburghii Rroxscaffold_7G00213870
rosa_samantha Rh2AG050500 Rh5AG329400 Rh6BG240900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 169
Acc65I GGTACC 1 cut(s) 157
AccB1I GGYRCC 2 cut(s) 157, 188
AccII CGCG 1 cut(s) 155
AciI CCGC 1 cut(s) 155
AclWI GGATC 1 cut(s) 41
AcsI RAATTY 1 cut(s) 166
AfaI GTAC 2 cut(s) 32, 159
AluBI AGCT 1 cut(s) 182
AluI AGCT 1 cut(s) 182
AlwI GGATC 1 cut(s) 41
ApoI RAATTY 1 cut(s) 166
Asp718I GGTACC 1 cut(s) 157
BanI GGYRCC 2 cut(s) 157, 188
BauI CACGAG 1 cut(s) 183
BfaI CTAG 1 cut(s) 179
BfuAI ACCTGC 1 cut(s) 169
BmiI GGNNCC 2 cut(s) 159, 190
BsaJI CCNNGG 1 cut(s) 192
Bse1I ACTGG 1 cut(s) 88
Bse3DI GCAATG 1 cut(s) 247
BseDI CCNNGG 1 cut(s) 192
BseMI GCAATG 1 cut(s) 247
BseMII CTCAG 1 cut(s) 87
BseNI ACTGG 1 cut(s) 88
Bsh1236I CGCG 1 cut(s) 155
BshNI GGYRCC 2 cut(s) 157, 188
BslFI GGGAC 1 cut(s) 159
BsmFI GGGAC 1 cut(s) 159
Bsp1407I TGTACA 1 cut(s) 30
Bsp143I GATC 2 cut(s) 46, 150
BspACI CCGC 1 cut(s) 155
BspCNI CTCAG 1 cut(s) 86
BspFNI CGCG 1 cut(s) 155
BspLI GGNNCC 2 cut(s) 159, 190
BspMI ACCTGC 1 cut(s) 169
BspPI GGATC 1 cut(s) 41
BspT107I GGYRCC 2 cut(s) 157, 188
BsrDI GCAATG 1 cut(s) 247
BsrGI TGTACA 1 cut(s) 30
BsrI ACTGG 1 cut(s) 88
BssECI CCNNGG 1 cut(s) 192
BssMI GATC 2 cut(s) 46, 150
BssSI CACGAG 1 cut(s) 183
BssT1I CCWWGG 1 cut(s) 192
Bst2BI CACGAG 1 cut(s) 183
Bst4CI ACNGT 1 cut(s) 62
BstAUI TGTACA 1 cut(s) 30
BstDEI CTNAG 2 cut(s) 73, 92
BstFNI CGCG 1 cut(s) 155
BstKTI GATC 2 cut(s) 49, 153
BstMBI GATC 2 cut(s) 46, 150
BstMWI GCNNNNNNNGC 2 cut(s) 161, 203
BstUI CGCG 1 cut(s) 155
BtsI GCAGTG 1 cut(s) 47
BtsIMutI CAGTG 1 cut(s) 47
BveI ACCTGC 1 cut(s) 169
Csp6I GTAC 2 cut(s) 31, 158
CspCI CAANNNNNGTGG 2 cut(s) 86, 121
CviAII CATG 2 cut(s) 9, 66
CviJI RGCY 6 cut(s) 27, 78, 102, 109, 182, 197
CviKI_1 RGCY 6 cut(s) 27, 78, 102, 109, 182, 197
CviQI GTAC 2 cut(s) 31, 158
DdeI CTNAG 2 cut(s) 73, 92
DpnI GATC 2 cut(s) 48, 152
DpnII GATC 2 cut(s) 46, 150
Eco130I CCWWGG 1 cut(s) 192
EcoT14I CCWWGG 1 cut(s) 192
ErhI CCWWGG 1 cut(s) 192
FaeI CATG 2 cut(s) 12, 69
FaiI YATR 5 cut(s) 10, 23, 67, 81, 256
FaqI GGGAC 1 cut(s) 159
FatI CATG 2 cut(s) 8, 65
FspBI CTAG 1 cut(s) 179
Hin1II CATG 2 cut(s) 12, 69
HinfI GANTC 2 cut(s) 71, 231
Hpy166II GTNNAC 1 cut(s) 89
Hpy188III TCNNGA 1 cut(s) 50
Hpy8I GTNNAC 1 cut(s) 89
HpyAV CCTTC 1 cut(s) 116
HpyCH4III ACNGT 1 cut(s) 62
HpyCH4V TGCA 2 cut(s) 40, 164
HpyF10VI GCNNNNNNNGC 2 cut(s) 161, 203
HpyF3I CTNAG 2 cut(s) 73, 92
Hsp92II CATG 2 cut(s) 12, 69
KpnI GGTACC 1 cut(s) 161
Kzo9I GATC 2 cut(s) 46, 150
LmnI GCTCC 1 cut(s) 113
LpnPI CCDG 7 cut(s) 41, 60, 63, 69, 88, 174, 236
MaeI CTAG 1 cut(s) 179
MaeIII GTNAC 1 cut(s) 68
MalI GATC 2 cut(s) 48, 152
MboI GATC 2 cut(s) 46, 150
MboII GAAGA 1 cut(s) 65
MfeI CAATTG 1 cut(s) 201
MluCI AATT 2 cut(s) 166, 201
MlyI GAGTC 1 cut(s) 65
MseI TTAA 1 cut(s) 134
MunI CAATTG 1 cut(s) 201
MvnI CGCG 1 cut(s) 155
MwoI GCNNNNNNNGC 2 cut(s) 161, 203
NdeII GATC 2 cut(s) 46, 150
NlaIII CATG 2 cut(s) 12, 69
NlaIV GGNNCC 2 cut(s) 159, 190
NmuCI GTSAC 1 cut(s) 68
PfeI GAWTC 1 cut(s) 231
PflFI GACNNNGTC 1 cut(s) 93
PleI GAGTC 1 cut(s) 65
PpsI GAGTC 1 cut(s) 65
PspN4I GGNNCC 2 cut(s) 159, 190
PsyI GACNNNGTC 1 cut(s) 93
RsaI GTAC 2 cut(s) 32, 159
RsaNI GTAC 2 cut(s) 31, 158
SaqAI TTAA 1 cut(s) 134
Sau3AI GATC 2 cut(s) 46, 150
SchI GAGTC 1 cut(s) 65
SetI ASST 4 cut(s) 163, 184, 194, 255
Sse9I AATT 2 cut(s) 166, 201
SsiI CCGC 1 cut(s) 155
SspMI CTAG 1 cut(s) 179
StyI CCWWGG 1 cut(s) 192
TaaI ACNGT 1 cut(s) 62
TasI AATT 2 cut(s) 166, 201
TatI WGTACW 1 cut(s) 30
TfiI GAWTC 1 cut(s) 231
Tru1I TTAA 1 cut(s) 134
Tru9I TTAA 1 cut(s) 134
TscAI CASTG 1 cut(s) 47
TseFI GTSAC 1 cut(s) 68
Tsp45I GTSAC 1 cut(s) 68
TspDTI ATGAA 1 cut(s) 54
TspRI CASTG 1 cut(s) 47
Tth111I GACNNNGTC 1 cut(s) 93
XapI RAATTY 1 cut(s) 166
XspI CTAG 1 cut(s) 179
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.