Rh5AG539500

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0015033
Physical Location & Seq
Reverse (-)
92773176 .. 92773727
552 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG539500.1

Sequence Viewer

Length: 552 bp
ATGGAATCATTGAAGTTTAGGAGACACCGTTTATTAGTAGATGAGGTGTGCATTTCTAGAATCAATCCAAGAGGCCGTCGAGCCATACAAGTTTTACTTTACCACTCAGGACAGATGGGTCAAGGTCAAATAATAAGACCATTCAACCAGGGGGGGAGTAGAACAGTGACCGTACTTTTGAAGAAGCATCCATATTCCATATCCCAAAGCACCCAAAGTCTGCAGAAGAAAGCAACAAGAGAGAGAGAGAGAGAGATGGTGTTCAATAGCCTGATGGTGGTGTCGGTGGCACACCTGTCAGCGGAGGCATGGCAACACGTGGCGTGCCTGCCGGAGCGTCTGAGCAGCCGGCAGCTGTTGGATCTTGTGTGCGTCATCCCTCTCCAGGAATTGGGTCGCCTCACTCTTTGCCTCTGGACCTTCCTCTGCGTCCCTCCTCCCGACTCCTACTTCTCCCGTTACTCCGACTCCTCCGATCACTCTGACTCCGACTCTGACTCCTCCACCACCGTCGATTACGATGACTATTACTACCACCCGCATTCCGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

183

Amino Acids

21.03

Weight (kDa)

7.71

Isoelectric Point (pI)

52.16

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015033)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G56520
fragaria_vesca FvH4_3g45760
malus_domestica MD03G1003400.v1.1 MD11G1003200.v1.1
prunus_persica Prupe.6G003100_v2.0.a1 Prupe.6G003100_v2.0.a1 Prupe.6G003100_v2.0.a1
pyrus_communis pycom03g00340 pycom11g00290
rosa_chinensis RchiOBHm_Chr5g0083831
rosa_multiflora Rmu_sc0001287.1_g000038 Rmu_sc0024007.1_g000004
rosa_roxburghii Rroxscaffold_1G00000760
rosa_rugosa Rorug05G0486300
rosa_samantha Rh5AG539500
rosa_wichuraiana Rw5G050150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 117
AccB7I CCANNNNNTGG 1 cut(s) 391
AciI CCGC 2 cut(s) 302, 539
AclWI GGATC 1 cut(s) 369
AcvI CACGTG 1 cut(s) 319
AfaI GTAC 1 cut(s) 174
AfiI CCNNNNNNNGG 4 cut(s) 277, 301, 385, 391
AflIII ACRYGT 1 cut(s) 316
AgsI TTSAA 4 cut(s) 13, 145, 181, 265
AjnI CCWGG 2 cut(s) 147, 384
AluBI AGCT 1 cut(s) 355
AluI AGCT 1 cut(s) 355
Alw26I GTCTC 1 cut(s) 16
AlwI GGATC 1 cut(s) 369
AoxI GGCC 1 cut(s) 73
ApeKI GCWGC 2 cut(s) 345, 352
ArsI GACNNNNNNTTYG 2 cut(s) 160, 192
AspS9I GGNCC 1 cut(s) 417
AvaII GGWCC 1 cut(s) 417
BbrPI CACGTG 1 cut(s) 319
BbvI GCAGC 2 cut(s) 357, 364
BccI CCATC 3 cut(s) 109, 250, 268
BceAI ACGGC 1 cut(s) 60
BciT130I CCWGG 2 cut(s) 149, 386
BcoDI GTCTC 1 cut(s) 16
BfaI CTAG 1 cut(s) 57
BfmI CTRYAG 1 cut(s) 221
BisI GCNGC 2 cut(s) 346, 353
BlsI GCNGC 2 cut(s) 347, 354
Bme1390I CCNGG 2 cut(s) 149, 386
Bme18I GGWCC 1 cut(s) 417
BmgT120I GGNCC 1 cut(s) 417
BmrFI CCNGG 2 cut(s) 149, 386
BmsI GCATC 1 cut(s) 196
BpmI CTGGAG 1 cut(s) 368
BsaAI YACGTR 1 cut(s) 319
BsaJI CCNNGG 1 cut(s) 148
BsaXI ACNNNNNCTCC 8 cut(s) 13, 43, 452, 470, 482, 482, 500, 512
Bsc4I CCNNNNNNNGG 4 cut(s) 277, 301, 385, 391
Bse118I RCCGGY 1 cut(s) 348
BseBI CCWGG 2 cut(s) 149, 386
BseDI CCNNGG 1 cut(s) 148
BseGI GGATG 2 cut(s) 187, 375
BseLI CCNNNNNNNGG 4 cut(s) 277, 301, 385, 391
BseMII CTCAG 2 cut(s) 120, 332
BseRI GAGGAG 3 cut(s) 426, 460, 490
BseXI GCAGC 2 cut(s) 357, 364
BshFI GGCC 1 cut(s) 75
BsiSI CCGG 2 cut(s) 332, 349
BslFI GGGAC 1 cut(s) 416
BslI CCNNNNNNNGG 4 cut(s) 277, 301, 385, 391
BsmAI GTCTC 1 cut(s) 16
BsmFI GGGAC 1 cut(s) 416
BsmI GAATGC 1 cut(s) 541
BsnI GGCC 1 cut(s) 75
Bsp143I GATC 2 cut(s) 361, 475
BspACI CCGC 2 cut(s) 302, 539
BspANI GGCC 1 cut(s) 75
BspCNI CTCAG 2 cut(s) 119, 333
BspMAI CTGCAG 1 cut(s) 225
BspPI GGATC 1 cut(s) 369
BsrFI RCCGGY 1 cut(s) 348
BssAI RCCGGY 1 cut(s) 348
BssECI CCNNGG 1 cut(s) 148
BssMI GATC 2 cut(s) 361, 475
Bst2UI CCWGG 2 cut(s) 149, 386
Bst4CI ACNGT 4 cut(s) 29, 166, 172, 511
BstBAI YACGTR 1 cut(s) 319
BstC8I GCNNGC 3 cut(s) 325, 329, 350
BstDEI CTNAG 2 cut(s) 106, 341
BstF5I GGATG 2 cut(s) 187, 375
BstKTI GATC 2 cut(s) 364, 478
BstMAI GTCTC 1 cut(s) 16
BstMBI GATC 2 cut(s) 361, 475
BstNI CCWGG 2 cut(s) 149, 386
BstSCI CCNGG 2 cut(s) 147, 384
BstSFI CTRYAG 1 cut(s) 221
BstV1I GCAGC 2 cut(s) 357, 364
BstX2I RGATCY 1 cut(s) 361
BstYI RGATCY 1 cut(s) 361
BsuRI GGCC 1 cut(s) 75
BtsCI GGATG 2 cut(s) 187, 375
BtsIMutI CAGTG 1 cut(s) 171
Cac8I GCNNGC 3 cut(s) 325, 329, 350
Cfr10I RCCGGY 1 cut(s) 348
Cfr13I GGNCC 1 cut(s) 417
CseI GACGC 3 cut(s) 326, 361, 418
Csp6I GTAC 1 cut(s) 173
CviAII CATG 1 cut(s) 309
CviJI RGCY 5 cut(s) 75, 83, 270, 348, 355
CviKI_1 RGCY 5 cut(s) 75, 83, 270, 348, 355
CviQI GTAC 1 cut(s) 173
DdeI CTNAG 2 cut(s) 106, 341
DpnI GATC 2 cut(s) 363, 477
DpnII GATC 2 cut(s) 361, 475
DrdI GACNNNNNNGTC 1 cut(s) 117
DseDI GACNNNNNNGTC 1 cut(s) 117
Eco47I GGWCC 1 cut(s) 417
Eco72I CACGTG 1 cut(s) 319
EcoRII CCWGG 2 cut(s) 147, 384
FaeI CATG 1 cut(s) 312
FaiI YATR 4 cut(s) 86, 193, 200, 310
FalI AAGNNNNNCTT 2 cut(s) 81, 113
FaqI GGGAC 1 cut(s) 416
FatI CATG 1 cut(s) 308
FauI CCCGC 1 cut(s) 546
Fnu4HI GCNGC 2 cut(s) 346, 353
FokI GGATG 2 cut(s) 174, 362
Fsp4HI GCNGC 2 cut(s) 346, 353
FspBI CTAG 1 cut(s) 57
GluI GCNGC 2 cut(s) 346, 353
GsuI CTGGAG 1 cut(s) 368
HaeIII GGCC 1 cut(s) 75
HapII CCGG 2 cut(s) 332, 349
HgaI GACGC 3 cut(s) 326, 361, 418
Hin1II CATG 1 cut(s) 312
HinfI GANTC 7 cut(s) 5, 60, 443, 467, 485, 491, 497
HpaII CCGG 2 cut(s) 332, 349
Hpy188I TCNGA 7 cut(s) 342, 466, 475, 484, 490, 496, 547
Hpy188III TCNNGA 4 cut(s) 57, 108, 415, 440
Hpy99I CGWCG 2 cut(s) 81, 515
HpyAV CCTTC 1 cut(s) 430
HpyCH4III ACNGT 4 cut(s) 29, 166, 172, 511
HpyCH4IV ACGT 1 cut(s) 318
HpyCH4V TGCA 2 cut(s) 51, 223
HpyF3I CTNAG 2 cut(s) 106, 341
HpySE526I ACGT 1 cut(s) 318
Hsp92II CATG 1 cut(s) 312
KroI GCCGGC 1 cut(s) 348
KroNI GCCGGC 1 cut(s) 350
Kzo9I GATC 2 cut(s) 361, 475
LmnI GCTCC 1 cut(s) 334
Lsp1109I GCAGC 2 cut(s) 357, 364
LweI GCATC 1 cut(s) 196
MaeI CTAG 1 cut(s) 57
MaeII ACGT 1 cut(s) 318
MaeIII GTNAC 2 cut(s) 166, 458
MalI GATC 2 cut(s) 363, 477
MboI GATC 2 cut(s) 361, 475
MboII GAAGA 2 cut(s) 193, 238
MflI RGATCY 1 cut(s) 361
MluCI AATT 1 cut(s) 389
MlyI GAGTC 5 cut(s) 437, 461, 479, 485, 491
MmeI TCCRAC 3 cut(s) 339, 489, 513
MroNI GCCGGC 1 cut(s) 348
MspA1I CMGCKG 2 cut(s) 302, 355
MspI CCGG 2 cut(s) 332, 349
MspR9I CCNGG 2 cut(s) 149, 386
Mva1269I GAATGC 1 cut(s) 541
MvaI CCWGG 2 cut(s) 149, 386
NaeI GCCGGC 1 cut(s) 350
NdeII GATC 2 cut(s) 361, 475
NgoMIV GCCGGC 1 cut(s) 348
NlaIII CATG 1 cut(s) 312
NmuCI GTSAC 1 cut(s) 166
PctI GAATGC 1 cut(s) 541
PdiI GCCGGC 1 cut(s) 350
PfeI GAWTC 2 cut(s) 5, 60
PflMI CCANNNNNTGG 1 cut(s) 391
PfoI TCCNGGA 1 cut(s) 384
PkrI GCNGC 2 cut(s) 347, 354
PleI GAGTC 5 cut(s) 437, 461, 479, 485, 491
PmaCI CACGTG 1 cut(s) 319
PmlI CACGTG 1 cut(s) 319
PpsI GAGTC 5 cut(s) 437, 461, 479, 485, 491
Ppu21I YACGTR 1 cut(s) 319
Psp6I CCWGG 2 cut(s) 147, 384
PspCI CACGTG 1 cut(s) 319
PspGI CCWGG 2 cut(s) 147, 384
PspPI GGNCC 1 cut(s) 417
PstI CTGCAG 1 cut(s) 225
PsuI RGATCY 1 cut(s) 361
PvuII CAGCTG 1 cut(s) 355
RsaI GTAC 1 cut(s) 174
RsaNI GTAC 1 cut(s) 173
SatI GCNGC 2 cut(s) 346, 353
Sau3AI GATC 2 cut(s) 361, 475
Sau96I GGNCC 1 cut(s) 417
SchI GAGTC 5 cut(s) 437, 461, 479, 485, 491
ScrFI CCNGG 2 cut(s) 149, 386
SetI ASST 6 cut(s) 48, 127, 297, 321, 357, 422
SfaNI GCATC 1 cut(s) 196
SfcI CTRYAG 1 cut(s) 221
SinI GGWCC 1 cut(s) 417
Sse9I AATT 1 cut(s) 389
SsiI CCGC 2 cut(s) 302, 539
SspMI CTAG 1 cut(s) 57
StyD4I CCNGG 2 cut(s) 147, 384
TaaI ACNGT 4 cut(s) 29, 166, 172, 511
TaiI ACGT 1 cut(s) 321
TaqI TCGA 2 cut(s) 79, 513
TasI AATT 1 cut(s) 389
TfiI GAWTC 2 cut(s) 5, 60
TscAI CASTG 1 cut(s) 171
TseFI GTSAC 1 cut(s) 166
TseI GCWGC 2 cut(s) 345, 352
Tsp45I GTSAC 1 cut(s) 166
TspRI CASTG 1 cut(s) 171
Van91I CCANNNNNTGG 1 cut(s) 391
VpaK11BI GGWCC 1 cut(s) 417
XbaI TCTAGA 1 cut(s) 56
XspI CTAG 1 cut(s) 57
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.