Rh5BG093400

Belongs to the eukaryotic initiation factor 4E family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0020565
Physical Location & Seq
Forward (+)
8339704 .. 8340051
348 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG093400.1

Sequence Viewer

Length: 348 bp
ATGCCATTCTCGAGAGTAAAGACTGAGATTTTGAGTTTTGTGAGAAAATATCCTCTAGTTCTTGTGGTGGCAAAGTTGATCGGAGATGAGGTAGGCTTCCCCTTCAACTCTTTACCAGTGCTTCCGGCCCTATTTCTGTACTTCCGACAGCTCTATAGGATGCCGACGTTGAGGGAAGCTGGTCACTCCCTTCTCCGGTCCGTACTGACCCTGCTGGGTTACCTGCTGATCATCTATGTTGTTGGTGGGTTGTTACTGAGGAGGAAGATGACGAAATGGCAGGTGGTGGATGAAGAACAACAAAAAGCAACCTGGAGTTGCTCCGGCGAGACCTCAGTCGGTAAATGA

Protein Analysis

115

Amino Acids

13.13

Weight (kDa)

9.86

Isoelectric Point (pI)

55.15

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0020565)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr6g0281831
rosa_multiflora Rmu_sc0003577.1_g000006
rosa_roxburghii Rroxscaffold_1G00063970
rosa_samantha Rh5AG096900 Rh5BG093400 Rh5DG092600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 271
Acc36I ACCTGC 2 cut(s) 231, 271
AfaI GTAC 2 cut(s) 140, 204
AfiI CCNNNNNNNGG 1 cut(s) 195
AgsI TTSAA 1 cut(s) 106
AjnI CCWGG 1 cut(s) 311
AluBI AGCT 2 cut(s) 151, 179
AluI AGCT 2 cut(s) 151, 179
Alw26I GTCTC 1 cut(s) 323
Ama87I CYCGRG 1 cut(s) 10
AoxI GGCC 1 cut(s) 126
ArsI GACNNNNNNTTYG 2 cut(s) 13, 45
AspS9I GGNCC 2 cut(s) 127, 198
AvaI CYCGRG 1 cut(s) 10
AvaII GGWCC 1 cut(s) 198
BciT130I CCWGG 1 cut(s) 313
BclI TGATCA 1 cut(s) 228
BcoDI GTCTC 1 cut(s) 323
BfaI CTAG 1 cut(s) 56
BfmI CTRYAG 1 cut(s) 154
BfuAI ACCTGC 2 cut(s) 231, 271
Bme1390I CCNGG 1 cut(s) 313
Bme18I GGWCC 1 cut(s) 198
BmeT110I CYCGRG 1 cut(s) 10
BmgT120I GGNCC 2 cut(s) 127, 198
BmrFI CCNGG 1 cut(s) 313
BmsI GCATC 1 cut(s) 150
BoxI GACNNNNGTC 1 cut(s) 335
BpmI CTGGAG 1 cut(s) 334
BsaI GGTCTC 1 cut(s) 323
BsaWI WCCGGW 1 cut(s) 195
BsaXI ACNNNNNCTCC 2 cut(s) 75, 105
Bsc4I CCNNNNNNNGG 1 cut(s) 195
Bse1I ACTGG 1 cut(s) 116
BseBI CCWGG 1 cut(s) 313
BseGI GGATG 2 cut(s) 165, 295
BseLI CCNNNNNNNGG 1 cut(s) 195
BseMII CTCAG 3 cut(s) 15, 248, 348
BseNI ACTGG 1 cut(s) 116
BseRI GAGGAG 1 cut(s) 274
BseYI CCCAGC 1 cut(s) 214
BshFI GGCC 1 cut(s) 128
BsiHKCI CYCGRG 1 cut(s) 10
BsiSI CCGG 3 cut(s) 125, 196, 324
BslI CCNNNNNNNGG 1 cut(s) 195
BsmAI GTCTC 1 cut(s) 323
BsnI GGCC 1 cut(s) 128
Bso31I GGTCTC 1 cut(s) 323
BsoBI CYCGRG 1 cut(s) 10
Bsp143I GATC 2 cut(s) 78, 228
BspANI GGCC 1 cut(s) 128
BspCNI CTCAG 3 cut(s) 16, 249, 347
BspMI ACCTGC 2 cut(s) 231, 271
BspTNI GGTCTC 1 cut(s) 323
BsrI ACTGG 1 cut(s) 116
BssMI GATC 2 cut(s) 78, 228
Bst2UI CCWGG 1 cut(s) 313
BstDEI CTNAG 3 cut(s) 24, 257, 334
BstEII GGTNACC 1 cut(s) 218
BstF5I GGATG 2 cut(s) 165, 295
BstKTI GATC 2 cut(s) 81, 231
BstMAI GTCTC 1 cut(s) 323
BstMBI GATC 2 cut(s) 78, 228
BstNI CCWGG 1 cut(s) 313
BstPAI GACNNNNGTC 1 cut(s) 335
BstPI GGTNACC 1 cut(s) 218
BstSCI CCNGG 1 cut(s) 311
BstSFI CTRYAG 1 cut(s) 154
BsuRI GGCC 1 cut(s) 128
BtsCI GGATG 2 cut(s) 165, 295
BtsIMutI CAGTG 1 cut(s) 123
BveI ACCTGC 2 cut(s) 231, 271
Cfr13I GGNCC 2 cut(s) 127, 198
CpoI CGGWCCG 1 cut(s) 198
Csp6I GTAC 2 cut(s) 139, 203
CspI CGGWCCG 1 cut(s) 198
CviJI RGCY 4 cut(s) 96, 128, 151, 179
CviKI_1 RGCY 4 cut(s) 96, 128, 151, 179
CviQI GTAC 2 cut(s) 139, 203
DdeI CTNAG 3 cut(s) 24, 257, 334
DpnI GATC 2 cut(s) 80, 230
DpnII GATC 2 cut(s) 78, 228
Eco31I GGTCTC 1 cut(s) 323
Eco47I GGWCC 1 cut(s) 198
Eco88I CYCGRG 1 cut(s) 10
Eco91I GGTNACC 1 cut(s) 218
EcoO65I GGTNACC 1 cut(s) 218
EcoRII CCWGG 1 cut(s) 311
FaiI YATR 2 cut(s) 156, 237
FbaI TGATCA 1 cut(s) 228
FokI GGATG 2 cut(s) 172, 302
FspBI CTAG 1 cut(s) 56
GsaI CCCAGC 1 cut(s) 218
GsuI CTGGAG 1 cut(s) 334
HaeIII GGCC 1 cut(s) 128
HapII CCGG 3 cut(s) 125, 196, 324
HpaII CCGG 3 cut(s) 125, 196, 324
Hpy188I TCNGA 2 cut(s) 83, 146
Hpy188III TCNNGA 2 cut(s) 10, 12
Hpy99I CGWCG 1 cut(s) 169
HpyAV CCTTC 2 cut(s) 112, 200
HpyCH4IV ACGT 1 cut(s) 167
HpyF3I CTNAG 3 cut(s) 24, 257, 334
HpySE526I ACGT 1 cut(s) 167
Ksp22I TGATCA 1 cut(s) 228
Kzo9I GATC 2 cut(s) 78, 228
LmnI GCTCC 1 cut(s) 326
LweI GCATC 1 cut(s) 150
MaeI CTAG 1 cut(s) 56
MaeII ACGT 1 cut(s) 167
MaeIII GTNAC 3 cut(s) 182, 218, 252
MalI GATC 2 cut(s) 80, 230
MboI GATC 2 cut(s) 78, 228
MboII GAAGA 2 cut(s) 277, 305
MmeI TCCRAC 1 cut(s) 169
MnlI CCTC 6 cut(s) 63, 82, 165, 252, 255, 343
MspI CCGG 3 cut(s) 125, 196, 324
MspR9I CCNGG 1 cut(s) 313
MvaI CCWGG 1 cut(s) 313
NdeII GATC 2 cut(s) 78, 228
NmuCI GTSAC 1 cut(s) 182
PaeR7I CTCGAG 1 cut(s) 10
PaqCI CACCTGC 1 cut(s) 271
PshAI GACNNNNGTC 1 cut(s) 335
Psp6I CCWGG 1 cut(s) 311
PspEI GGTNACC 1 cut(s) 218
PspFI CCCAGC 1 cut(s) 214
PspGI CCWGG 1 cut(s) 311
PspPI GGNCC 2 cut(s) 127, 198
RsaI GTAC 2 cut(s) 140, 204
RsaNI GTAC 2 cut(s) 139, 203
Rsr2I CGGWCCG 1 cut(s) 198
RsrII CGGWCCG 1 cut(s) 198
Sau3AI GATC 2 cut(s) 78, 228
Sau96I GGNCC 2 cut(s) 127, 198
ScrFI CCNGG 1 cut(s) 313
SetI ASST 8 cut(s) 93, 153, 170, 181, 225, 285, 314, 335
SfaNI GCATC 1 cut(s) 150
SfcI CTRYAG 1 cut(s) 154
Sfr274I CTCGAG 1 cut(s) 10
SinI GGWCC 1 cut(s) 198
SlaI CTCGAG 1 cut(s) 10
SmlI CTYRAG 1 cut(s) 10
SmoI CTYRAG 1 cut(s) 10
SspMI CTAG 1 cut(s) 56
StyD4I CCNGG 1 cut(s) 311
TaiI ACGT 1 cut(s) 170
TaqI TCGA 1 cut(s) 11
TatI WGTACW 1 cut(s) 138
TscAI CASTG 1 cut(s) 123
TseFI GTSAC 1 cut(s) 182
Tsp45I GTSAC 1 cut(s) 182
TspDTI ATGAA 1 cut(s) 306
TspGWI ACGGA 1 cut(s) 190
TspRI CASTG 1 cut(s) 123
VpaK11BI GGWCC 1 cut(s) 198
XhoI CTCGAG 1 cut(s) 10
XspI CTAG 1 cut(s) 56
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.