Rh5BG205500
ERF Family

Belongs to the protein kinase superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0019491
Physical Location & Seq
Reverse (-)
23323662 .. 23341642
17981 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG205500.1

Sequence Viewer

Length: 669 bp
ATGAGAGGGCTTAGTGGTCATTGTGGGAATGAGGTTTCTTCTTGCTTTCAAACACTGTCATGGAACCTGCGCATGAAGATTGCACTCGGTGCTGCTAAGGCTCTAGCATTTCTTCATCATGAGGCAAAATGCATATATGTGGACTTTGAGACATCTAAGATCCTTCTTGATTCAAACTATAATGTCAAAATATATGGTTACGGTTTAGCCCAGGAAGGGCCTGAAGGTTATAGAATCCGTCTCTTGGATATGGATAAACTTCCTTGGTATGAGGATAAAGTGATTGAGCACAACGGCGGGCCTTATAGAGCTCCTGAGTATATATTTGCAGGTGCAGATCACTTGACCCCCAGAAGCGATGTGTACAGTTTTGGAGTTGTTCTGCTCGAAATCTTGGCAGGCCGACGAGCAATTGACAGTAACCGGCTAGTGGCAGAACGTGATTTAGTTCGGTTTGTTATTACCAAGGTTCCCAGACAAATATGTCATCAAAAAGAAAAAAAGGTTCTCAGACAAATAGTTCGCCAAATTATTGACAGTCGTATGGAAGGCCAGTACCCTTGGTCTGGAGCTGTCAAAGCACTTGAACTTGCAAGACAATGCCTGTCAAATTACTCCAAGCATAGGCCAGACATGCATCAGGTGGTAGAAATATTGGAGAACCTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0002682 GO:0002684 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004675 GO:0004888 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006952 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007178 GO:0008150 GO:0008152 GO:0009605 GO:0009607 GO:0009620 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019199 GO:0019538 GO:0023052 GO:0031347 GO:0031349 GO:0031974 GO:0031981 GO:0035821 GO:0036211 GO:0038023 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0044003 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044403 GO:0044419 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0045088 GO:0045089 GO:0046777 GO:0048518 GO:0048583 GO:0048584 GO:0050776 GO:0050778 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051701 GO:0051704 GO:0051707 GO:0051716 GO:0051817 GO:0052031 GO:0052033 GO:0052166 GO:0052167 GO:0052169 GO:0052173 GO:0052200 GO:0052255 GO:0052257 GO:0052305 GO:0052306 GO:0052308 GO:0052509 GO:0052510 GO:0052552 GO:0052553 GO:0052555 GO:0052556 GO:0052564 GO:0052572 GO:0060089 GO:0065007 GO:0070013 GO:0071704 GO:0071944 GO:0075136 GO:0080134 GO:0098542 GO:0140096 GO:1901564
KEGG Pathways
Metabolic & Signaling
ko04010 ko04064 ko04620 ko04621 ko04624 ko04722 ko05133 ko05140 ko05142 ko05145 ko05152 ko05162 ko05164 map04010 map04064 map04620 map04621 map04624 map04722 map05133 map05140 map05142 map05145 map05152 map05162 map05164
Pfam Domains
Protein Families
Pkinase Pkinase_Tyr

Protein Analysis

222

Amino Acids

25.42

Weight (kDa)

8.74

Isoelectric Point (pI)

31.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK_Tyr_Ser-Thr PF07714 17 - 219 4e-13 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 25 - 217 2.5e-16 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019491)

Species Orthologous Gene IDs
rosa_laevigata RLG00000027273
rosa_multiflora Rmu_sc0000210.1_g000009 Rmu_sc0004181.1_g000006
rosa_roxburghii Rroxscaffold_1G00050410
rosa_samantha Rh5AG206800 Rh5BG205500 Rh5CG227500 Rh5DG209200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 320
AasI GACNNNNNNGTC 1 cut(s) 483
Acc16I TGCGCA 1 cut(s) 71
Acc36I ACCTGC 2 cut(s) 75, 320
AciI CCGC 1 cut(s) 297
AclWI GGATC 1 cut(s) 154
AcuI CTGAAG 1 cut(s) 243
AdeI CACNNNGTG 1 cut(s) 89
AfaI GTAC 2 cut(s) 365, 557
AfiI CCNNNNNNNGG 5 cut(s) 216, 244, 430, 566, 624
AgsI TTSAA 3 cut(s) 50, 174, 587
AjnI CCWGG 1 cut(s) 210
AluBI AGCT 2 cut(s) 311, 572
AluI AGCT 2 cut(s) 311, 572
Alw21I GWGCWC 2 cut(s) 291, 313
Alw26I GTCTC 2 cut(s) 143, 245
AlwI GGATC 1 cut(s) 154
AoxI GGCC 5 cut(s) 218, 299, 400, 550, 626
ApeKI GCWGC 1 cut(s) 92
ArsI GACNNNNNNTTYG 2 cut(s) 504, 536
AspLEI GCGC 1 cut(s) 72
AspS9I GGNCC 2 cut(s) 218, 299
BanII GRGCYC 1 cut(s) 313
Bbv12I GWGCWC 2 cut(s) 291, 313
BbvI GCAGC 1 cut(s) 79
BceAI ACGGC 1 cut(s) 310
BciT130I CCWGG 1 cut(s) 212
BcoDI GTCTC 2 cut(s) 143, 245
BfaI CTAG 2 cut(s) 104, 428
BfuAI ACCTGC 2 cut(s) 75, 320
BisI GCNGC 1 cut(s) 93
BlsI GCNGC 1 cut(s) 94
Bme1390I CCNGG 1 cut(s) 212
BmgT120I GGNCC 2 cut(s) 218, 299
BmiI GGNNCC 2 cut(s) 65, 471
BmrFI CCNGG 1 cut(s) 212
BmsI GCATC 1 cut(s) 646
BpmI CTGGAG 1 cut(s) 588
Bpu10I CCTNAGC 1 cut(s) 96
BsaJI CCNNGG 4 cut(s) 210, 263, 465, 560
Bsc4I CCNNNNNNNGG 5 cut(s) 216, 244, 430, 566, 624
Bse118I RCCGGY 1 cut(s) 423
Bse1I ACTGG 1 cut(s) 553
BseBI CCWGG 1 cut(s) 212
BseDI CCNNGG 4 cut(s) 210, 263, 465, 560
BseLI CCNNNNNNNGG 5 cut(s) 216, 244, 430, 566, 624
BseMII CTCAG 2 cut(s) 306, 523
BseNI ACTGG 1 cut(s) 553
BseXI GCAGC 1 cut(s) 79
BsgI GTGCAG 1 cut(s) 354
BshFI GGCC 5 cut(s) 220, 301, 402, 552, 628
BsiHKAI GWGCWC 2 cut(s) 291, 313
BsiSI CCGG 1 cut(s) 424
BslI CCNNNNNNNGG 5 cut(s) 216, 244, 430, 566, 624
BsmAI GTCTC 2 cut(s) 143, 245
BsmBI CGTCTC 1 cut(s) 245
BsnI GGCC 5 cut(s) 220, 301, 402, 552, 628
Bsp1286I GDGCHC 2 cut(s) 291, 313
Bsp1407I TGTACA 1 cut(s) 363
Bsp143I GATC 2 cut(s) 159, 337
BspACI CCGC 1 cut(s) 297
BspANI GGCC 5 cut(s) 220, 301, 402, 552, 628
BspCNI CTCAG 2 cut(s) 307, 522
BspHI TCATGA 1 cut(s) 118
BspLI GGNNCC 2 cut(s) 65, 471
BspMI ACCTGC 2 cut(s) 75, 320
BspPI GGATC 1 cut(s) 154
BsrFI RCCGGY 1 cut(s) 423
BsrGI TGTACA 1 cut(s) 363
BsrI ACTGG 1 cut(s) 553
BssAI RCCGGY 1 cut(s) 423
BssECI CCNNGG 4 cut(s) 210, 263, 465, 560
BssMI GATC 2 cut(s) 159, 337
BssT1I CCWWGG 3 cut(s) 263, 465, 560
Bst2UI CCWGG 1 cut(s) 212
Bst4CI ACNGT 5 cut(s) 57, 203, 368, 419, 539
BstAPI GCANNNNNTGC 1 cut(s) 89
BstAUI TGTACA 1 cut(s) 363
BstC8I GCNNGC 2 cut(s) 299, 400
BstDEI CTNAG 5 cut(s) 11, 96, 156, 315, 509
BstHHI GCGC 1 cut(s) 72
BstKTI GATC 2 cut(s) 162, 340
BstMAI GTCTC 2 cut(s) 143, 245
BstMBI GATC 2 cut(s) 159, 337
BstMWI GCNNNNNNNGC 4 cut(s) 89, 98, 578, 634
BstNI CCWGG 1 cut(s) 212
BstNSI RCATGY 1 cut(s) 637
BstSCI CCNGG 1 cut(s) 210
BstV1I GCAGC 1 cut(s) 79
BstX2I RGATCY 1 cut(s) 159
BstYI RGATCY 1 cut(s) 159
BsuRI GGCC 5 cut(s) 220, 301, 402, 552, 628
BtgZI GCGATG 1 cut(s) 372
BtsIMutI CAGTG 1 cut(s) 53
BveI ACCTGC 2 cut(s) 75, 320
Cac8I GCNNGC 2 cut(s) 299, 400
CciI TCATGA 1 cut(s) 118
CfoI GCGC 1 cut(s) 72
Cfr10I RCCGGY 1 cut(s) 423
Cfr13I GGNCC 2 cut(s) 218, 299
Csp6I GTAC 2 cut(s) 364, 556
CviAII CATG 4 cut(s) 60, 73, 119, 634
CviQI GTAC 2 cut(s) 364, 556
DdeI CTNAG 5 cut(s) 11, 96, 156, 315, 509
DpnI GATC 2 cut(s) 161, 339
DpnII GATC 2 cut(s) 159, 337
DraIII CACNNNGTG 1 cut(s) 89
DrdI GACNNNNNNGTC 1 cut(s) 483
DseDI GACNNNNNNGTC 1 cut(s) 483
Ecl136II GAGCTC 1 cut(s) 311
Eco130I CCWWGG 3 cut(s) 263, 465, 560
Eco24I GRGCYC 1 cut(s) 313
Eco53kI GAGCTC 1 cut(s) 311
Eco57I CTGAAG 1 cut(s) 243
EcoICRI GAGCTC 1 cut(s) 311
EcoO109I RGGNCCY 1 cut(s) 218
EcoRII CCWGG 1 cut(s) 210
EcoT14I CCWWGG 3 cut(s) 263, 465, 560
EcoT22I ATGCAT 2 cut(s) 134, 639
EcoT38I GRGCYC 1 cut(s) 313
ErhI CCWWGG 3 cut(s) 263, 465, 560
Esp3I CGTCTC 1 cut(s) 245
FaeI CATG 4 cut(s) 63, 76, 122, 637
FatI CATG 4 cut(s) 59, 72, 118, 633
FauI CCCGC 1 cut(s) 290
Fnu4HI GCNGC 1 cut(s) 93
FriOI GRGCYC 1 cut(s) 313
Fsp4HI GCNGC 1 cut(s) 93
FspBI CTAG 2 cut(s) 104, 428
FspI TGCGCA 1 cut(s) 71
GlaI GCGC 1 cut(s) 71
GluI GCNGC 1 cut(s) 93
GsuI CTGGAG 1 cut(s) 588
HaeIII GGCC 5 cut(s) 220, 301, 402, 552, 628
HapII CCGG 1 cut(s) 424
HhaI GCGC 1 cut(s) 72
Hin1II CATG 4 cut(s) 63, 76, 122, 637
Hin6I GCGC 1 cut(s) 70
HinP1I GCGC 1 cut(s) 70
HinfI GANTC 2 cut(s) 170, 234
HpaII CCGG 1 cut(s) 424
Hpy166II GTNNAC 2 cut(s) 142, 364
Hpy188I TCNGA 1 cut(s) 512
Hpy188III TCNNGA 4 cut(s) 119, 167, 314, 567
Hpy8I GTNNAC 2 cut(s) 142, 364
Hpy99I CGWCG 1 cut(s) 408
HpyAV CCTTC 4 cut(s) 173, 209, 218, 542
HpyCH4III ACNGT 5 cut(s) 57, 203, 368, 419, 539
HpyCH4IV ACGT 1 cut(s) 439
HpyCH4V TGCA 6 cut(s) 83, 132, 329, 335, 593, 637
HpyF10VI GCNNNNNNNGC 4 cut(s) 89, 98, 578, 634
HpyF3I CTNAG 5 cut(s) 11, 96, 156, 315, 509
HpySE526I ACGT 1 cut(s) 439
Hsp92II CATG 4 cut(s) 63, 76, 122, 637
HspAI GCGC 1 cut(s) 70
Kzo9I GATC 2 cut(s) 159, 337
LmnI GCTCC 2 cut(s) 316, 569
Lsp1109I GCAGC 1 cut(s) 79
LweI GCATC 1 cut(s) 646
MaeI CTAG 2 cut(s) 104, 428
MaeII ACGT 1 cut(s) 439
MaeIII GTNAC 2 cut(s) 197, 419
MalI GATC 2 cut(s) 161, 339
MboI GATC 2 cut(s) 159, 337
MboII GAAGA 3 cut(s) 30, 88, 104
MfeI CAATTG 1 cut(s) 411
MflI RGATCY 1 cut(s) 159
MhlI GDGCHC 2 cut(s) 291, 313
MluCI AATT 3 cut(s) 411, 528, 610
MnlI CCTC 3 cut(s) 25, 115, 265
Mph1103I ATGCAT 2 cut(s) 134, 639
MslI CAYNNNNRTG 2 cut(s) 58, 137
MspI CCGG 1 cut(s) 424
MspR9I CCNGG 1 cut(s) 212
MunI CAATTG 1 cut(s) 411
MvaI CCWGG 1 cut(s) 212
MwoI GCNNNNNNNGC 4 cut(s) 89, 98, 578, 634
NdeII GATC 2 cut(s) 159, 337
NlaIII CATG 4 cut(s) 63, 76, 122, 637
NlaIV GGNNCC 2 cut(s) 65, 471
NsbI TGCGCA 1 cut(s) 71
NsiI ATGCAT 2 cut(s) 134, 639
NspI RCATGY 1 cut(s) 637
PagI TCATGA 1 cut(s) 118
PaqCI CACCTGC 1 cut(s) 320
PfeI GAWTC 2 cut(s) 170, 234
PkrI GCNGC 1 cut(s) 94
Psp124BI GAGCTC 1 cut(s) 313
Psp6I CCWGG 1 cut(s) 210
PspGI CCWGG 1 cut(s) 210
PspN4I GGNNCC 2 cut(s) 65, 471
PspPI GGNCC 2 cut(s) 218, 299
PsuI RGATCY 1 cut(s) 159
RsaI GTAC 2 cut(s) 365, 557
RsaNI GTAC 2 cut(s) 364, 556
RseI CAYNNNNRTG 2 cut(s) 58, 137
SacI GAGCTC 1 cut(s) 313
SatI GCNGC 1 cut(s) 93
Sau3AI GATC 2 cut(s) 159, 337
Sau96I GGNCC 2 cut(s) 218, 299
ScrFI CCNGG 1 cut(s) 212
SduI GDGCHC 2 cut(s) 291, 313
SfaNI GCATC 1 cut(s) 646
SmiMI CAYNNNNRTG 2 cut(s) 58, 137
Sse9I AATT 3 cut(s) 411, 528, 610
SsiI CCGC 1 cut(s) 297
SspI AATATT 1 cut(s) 654
SspMI CTAG 2 cut(s) 104, 428
SstI GAGCTC 1 cut(s) 313
StyD4I CCNGG 1 cut(s) 210
StyI CCWWGG 3 cut(s) 263, 465, 560
TaaI ACNGT 5 cut(s) 57, 203, 368, 419, 539
TaiI ACGT 1 cut(s) 442
TaqI TCGA 1 cut(s) 387
TasI AATT 3 cut(s) 411, 528, 610
TatI WGTACW 1 cut(s) 363
TfiI GAWTC 2 cut(s) 170, 234
TscAI CASTG 1 cut(s) 60
TseI GCWGC 1 cut(s) 92
TspDTI ATGAA 2 cut(s) 89, 104
TspGWI ACGGA 1 cut(s) 227
TspRI CASTG 1 cut(s) 60
XceI RCATGY 1 cut(s) 637
XspI CTAG 2 cut(s) 104, 428
Zsp2I ATGCAT 2 cut(s) 134, 639
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.