Rh5BG283400

Trigger factor-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0011134
Physical Location & Seq
Forward (+)
36022516 .. 36029069
6554 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG283400.1

Sequence Viewer

Length: 1350 bp
ATGGAGCTCTGCCTCACTGCCATCAATGGTGGCTCCACAGCGGTCCTCAACTCAAACCCATCACGCTCCTCACCCTCCCTTCTTCTCCCCAAAAACCCTTCTTCTCTCCAACTCTACACTTCTCGCAGACCCCCCTCTCTTTCCCGCCAATTCCACCCTCTCCAATTCGCGGTCTCGTCCTCTTCTTCCACCGCTACCACCTCCGAGATAGACCAACTTCCGGCCGACATCAGCGTCACAGAGACCCAACAACCCAATTCCAGAGTGAAGTTGAGCATACAAGTGCCGCCGGAGGTCTGCGATGACTGTTACAAAAAGGTTATAGTCGAGTTCACGAAGCAAGCAAAGGTTCCGGGGTTCCGGCCTGGAAAGAGGGTGCCGGAGAGTATTCTTGTGAGTTATGTAGGGAAGAAAAGTGTAGACAAGGCTGCCATTGAGTCTATTCTGAGGAGGACCCTTCCACATGCCATGTCTTCAATGACCGGCACGGCTTTGACAGACTCAGTCAGCATTCTGACCAAATTTTCTGAGCTAGAGCAGGCCTATTCTTCTGGCACCTCTCTCAGATATGATGTGATTGTTGATGTAGCACCAGAAGTGAAATGGATTCCTGAGAATGGATACAAGAATTTGAAGGTCGCCGTTGAGATAGACAGTGACATAGATGCTCAAAAGGTTTCTGAACAAGAATTAAGACGCCGTCACAAGTCCCTAGGGGCTTTAAGAATTGTAACTGACAGAGGACTACAGGTTGGCGATCTTGTTGTCCTTGATATATCTGCAACAACAATAAATCAAGACGAATCAAATGTTCAAAATATTCCATCTGCAGAGAGTAAAGGTTATCATTTTGATACAGAAGATGGTGATGAACTAATTCCCGGTTTCCTTGATTCGATAATTGGAATTCAACGAGGTGAAACAAAGTCTTTTCCTCTTGTATTTCCTGAATCATGGGCACAGGAAAATCTCCGAGGAATTCATGCTCAATTCAATGTTGAATGCAAGGAGTTATTTTACAGAGATTTACCCGAATTGGATGACTCCCTTGCTCCTAAGCTTCTTCCTGGATGCACCACCCTGGAACAGGTCAAGGAATCACTCTTGCAAAAATGCCAGGAAGTAGAGCAAACAGCTAGAGAGCAAGCAGCAGATAATGCCATTCTAGATCAGCTTTGCAAGGTGATTTTAATTTCTAAGATTATATTCAATTTTTGCAACCTTTTGTTGGTCTTTACCTTTTCAAACAAGGCAGTGCATACCCTTTTCATCTGTGGGAGCGCGCATACAGTTAAAGTGTTTGATATACACATAAGCATAAACCTTAAGTTGACTGGAGTAGGACCCTAA

Protein Analysis

449

Amino Acids

49.44

Weight (kDa)

5.62

Isoelectric Point (pI)

52.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Trigger_N PF05697 78 - 228 2.3e-21 Bacterial trigger factor protein (TF)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 233
AccB1I GGYRCC 2 cut(s) 376, 554
AccI GTMKAC 1 cut(s) 420
AccII CGCG 2 cut(s) 170, 1283
AciI CCGC 5 cut(s) 41, 145, 170, 192, 287
AcoI YGGCCR 1 cut(s) 222
AcsI RAATTY 4 cut(s) 521, 628, 906, 978
AcyI GRCGYC 1 cut(s) 697
AfiI CCNNNNNNNGG 5 cut(s) 169, 220, 617, 1087, 1228
AflII CTTAAG 1 cut(s) 1325
AgsI TTSAA 8 cut(s) 477, 634, 815, 911, 994, 1001, 1210, 1245
AjnI CCWGG 4 cut(s) 364, 1066, 1080, 1116
AluBI AGCT 5 cut(s) 7, 532, 1060, 1136, 1174
AluI AGCT 5 cut(s) 7, 532, 1060, 1136, 1174
Alw21I GWGCWC 1 cut(s) 9
Alw26I GTCTC 2 cut(s) 178, 236
AoxI GGCC 3 cut(s) 222, 362, 540
ApeKI GCWGC 2 cut(s) 428, 1148
ApoI RAATTY 4 cut(s) 521, 628, 906, 978
Asp700I GAANNNNTTC 1 cut(s) 876
AspA2I CCTAGG 1 cut(s) 712
AspLEI GCGC 2 cut(s) 1283, 1285
AspS9I GGNCC 3 cut(s) 43, 453, 1343
AsuC2I CCSGG 2 cut(s) 354, 882
AsuHPI GGTGA 4 cut(s) 63, 878, 929, 1195
AvaII GGWCC 3 cut(s) 43, 453, 1343
AvrII CCTAGG 1 cut(s) 712
BaeGI GKGCMC 1 cut(s) 961
BanI GGYRCC 2 cut(s) 376, 554
BanII GRGCYC 1 cut(s) 9
BbsI GAAGAC 1 cut(s) 465
Bbv12I GWGCWC 1 cut(s) 9
BbvI GCAGC 2 cut(s) 415, 1160
BccI CCATC 4 cut(s) 29, 67, 832, 857
BceAI ACGGC 3 cut(s) 504, 626, 684
BciT130I CCWGG 4 cut(s) 366, 1068, 1082, 1118
BciVI GTATCC 1 cut(s) 614
BcnI CCSGG 2 cut(s) 354, 882
BcoDI GTCTC 2 cut(s) 178, 236
BfaI CTAG 4 cut(s) 533, 713, 1137, 1166
BfmI CTRYAG 2 cut(s) 746, 828
BfrI CTTAAG 1 cut(s) 1325
BfuI GTATCC 1 cut(s) 614
BisI GCNGC 3 cut(s) 287, 429, 1149
BlnI CCTAGG 1 cut(s) 712
BlsI GCNGC 3 cut(s) 288, 430, 1150
Bme1390I CCNGG 6 cut(s) 354, 366, 882, 1068, 1082, 1118
Bme18I GGWCC 3 cut(s) 43, 453, 1343
BmgT120I GGNCC 3 cut(s) 43, 453, 1343
BmiI GGNNCC 7 cut(s) 34, 351, 359, 378, 455, 556, 1345
BmrFI CCNGG 6 cut(s) 354, 366, 882, 1068, 1082, 1118
BmsI GCATC 2 cut(s) 655, 1061
BpiI GAAGAC 1 cut(s) 465
Bpu10I CCTNAGC 1 cut(s) 1056
BpuMI CCSGG 2 cut(s) 354, 882
BsaHI GRCGYC 1 cut(s) 697
BsaI GGTCTC 2 cut(s) 178, 236
BsaJI CCNNGG 4 cut(s) 353, 712, 973, 1080
Bsc4I CCNNNNNNNGG 5 cut(s) 169, 220, 617, 1087, 1228
Bse118I RCCGGY 1 cut(s) 482
Bse1I ACTGG 1 cut(s) 1339
BseBI CCWGG 4 cut(s) 366, 1068, 1082, 1118
BseDI CCNNGG 4 cut(s) 353, 712, 973, 1080
BseGI GGATG 2 cut(s) 1045, 1076
BseLI CCNNNNNNNGG 5 cut(s) 169, 220, 617, 1087, 1228
BseMII CTCAG 5 cut(s) 437, 516, 519, 577, 603
BseNI ACTGG 1 cut(s) 1339
BsePI GCGCGC 1 cut(s) 1281
BseRI GAGGAG 2 cut(s) 58, 463
BseSI GKGCMC 1 cut(s) 961
BseX3I CGGCCG 1 cut(s) 222
BseXI GCAGC 2 cut(s) 415, 1160
Bsh1236I CGCG 2 cut(s) 170, 1283
Bsh1285I CGRYCG 1 cut(s) 225
BshFI GGCC 3 cut(s) 224, 364, 542
BshNI GGYRCC 2 cut(s) 376, 554
BsiEI CGRYCG 1 cut(s) 225
BsiHKAI GWGCWC 1 cut(s) 9
BsiSI CCGG 7 cut(s) 221, 290, 353, 361, 380, 483, 882
BslFI GGGAC 1 cut(s) 694
BslI CCNNNNNNNGG 5 cut(s) 169, 220, 617, 1087, 1228
BsmAI GTCTC 2 cut(s) 178, 236
BsmFI GGGAC 1 cut(s) 694
BsmI GAATGC 2 cut(s) 510, 1007
BsnI GGCC 3 cut(s) 224, 364, 542
Bso31I GGTCTC 2 cut(s) 178, 236
Bsp1286I GDGCHC 2 cut(s) 9, 961
Bsp143I GATC 2 cut(s) 757, 1168
BspACI CCGC 5 cut(s) 41, 145, 170, 192, 287
BspANI GGCC 3 cut(s) 224, 364, 542
BspCNI CTCAG 5 cut(s) 438, 515, 520, 576, 604
BspFNI CGCG 2 cut(s) 170, 1283
BspLI GGNNCC 7 cut(s) 34, 351, 359, 378, 455, 556, 1345
BspMAI CTGCAG 1 cut(s) 832
BspT107I GGYRCC 2 cut(s) 376, 554
BspTI CTTAAG 1 cut(s) 1325
BspTNI GGTCTC 2 cut(s) 178, 236
BsrFI RCCGGY 1 cut(s) 482
BsrI ACTGG 1 cut(s) 1339
BssAI RCCGGY 1 cut(s) 482
BssECI CCNNGG 4 cut(s) 353, 712, 973, 1080
BssHII GCGCGC 1 cut(s) 1281
BssMI GATC 2 cut(s) 757, 1168
BssNI GRCGYC 1 cut(s) 697
BssT1I CCWWGG 1 cut(s) 712
Bst2UI CCWGG 4 cut(s) 366, 1068, 1082, 1118
Bst4CI ACNGT 3 cut(s) 308, 656, 1291
Bst6I CTCTTC 1 cut(s) 187
BstACI GRCGYC 1 cut(s) 697
BstAFI CTTAAG 1 cut(s) 1325
BstAPI GCANNNNNTGC 1 cut(s) 1157
BstC8I GCNNGC 4 cut(s) 342, 540, 1146, 1283
BstDEI CTNAG 7 cut(s) 446, 502, 528, 563, 612, 1056, 1197
BstENI CCTNNNNNAGG 1 cut(s) 1085
BstF5I GGATG 2 cut(s) 1045, 1076
BstFNI CGCG 2 cut(s) 170, 1283
BstHHI GCGC 2 cut(s) 1283, 1285
BstKTI GATC 2 cut(s) 760, 1171
BstMAI GTCTC 2 cut(s) 178, 236
BstMBI GATC 2 cut(s) 757, 1168
BstMCI CGRYCG 1 cut(s) 225
BstMWI GCNNNNNNNGC 1 cut(s) 1157
BstNI CCWGG 4 cut(s) 366, 1068, 1082, 1118
BstNSI RCATGY 1 cut(s) 467
BstSCI CCNGG 6 cut(s) 352, 364, 880, 1066, 1080, 1116
BstSFI CTRYAG 2 cut(s) 746, 828
BstSLI GKGCMC 1 cut(s) 961
BstUI CGCG 2 cut(s) 170, 1283
BstV1I GCAGC 2 cut(s) 415, 1160
BstV2I GAAGAC 1 cut(s) 465
BstZI CGGCCG 1 cut(s) 222
BsuI GTATCC 1 cut(s) 614
BsuRI GGCC 3 cut(s) 224, 364, 542
BtgZI GCGATG 1 cut(s) 315
BtsCI GGATG 2 cut(s) 1045, 1076
BtsI GCAGTG 2 cut(s) 15, 1260
BtsIMutI CAGTG 3 cut(s) 15, 661, 1260
Cac8I GCNNGC 4 cut(s) 342, 540, 1146, 1283
CfoI GCGC 2 cut(s) 1283, 1285
Cfr10I RCCGGY 1 cut(s) 482
Cfr13I GGNCC 3 cut(s) 43, 453, 1343
CseI GACGC 2 cut(s) 223, 705
CviAII CATG 4 cut(s) 464, 469, 954, 983
DdeI CTNAG 7 cut(s) 446, 502, 528, 563, 612, 1056, 1197
DpnI GATC 2 cut(s) 759, 1170
DpnII GATC 2 cut(s) 757, 1168
DrdI GACNNNNNNGTC 1 cut(s) 233
DseDI GACNNNNNNGTC 1 cut(s) 233
EaeI YGGCCR 1 cut(s) 222
EagI CGGCCG 1 cut(s) 222
Eam1104I CTCTTC 1 cut(s) 187
EarI CTCTTC 1 cut(s) 187
Ecl136II GAGCTC 1 cut(s) 7
EclXI CGGCCG 1 cut(s) 222
Eco130I CCWWGG 1 cut(s) 712
Eco147I AGGCCT 1 cut(s) 542
Eco24I GRGCYC 1 cut(s) 9
Eco31I GGTCTC 2 cut(s) 178, 236
Eco47I GGWCC 3 cut(s) 43, 453, 1343
Eco52I CGGCCG 1 cut(s) 222
Eco53kI GAGCTC 1 cut(s) 7
EcoICRI GAGCTC 1 cut(s) 7
EcoNI CCTNNNNNAGG 1 cut(s) 1085
EcoO109I RGGNCCY 2 cut(s) 453, 1343
EcoRI GAATTC 2 cut(s) 906, 978
EcoRII CCWGG 4 cut(s) 364, 1066, 1080, 1116
EcoT14I CCWWGG 1 cut(s) 712
EcoT38I GRGCYC 1 cut(s) 9
ErhI CCWWGG 1 cut(s) 712
FaeI CATG 4 cut(s) 467, 472, 957, 986
FaqI GGGAC 1 cut(s) 694
FatI CATG 4 cut(s) 463, 468, 953, 982
FauI CCCGC 1 cut(s) 152
FblI GTMKAC 1 cut(s) 420
Fnu4HI GCNGC 3 cut(s) 287, 429, 1149
FokI GGATG 2 cut(s) 1052, 1083
FriOI GRGCYC 1 cut(s) 9
Fsp4HI GCNGC 3 cut(s) 287, 429, 1149
FspBI CTAG 4 cut(s) 533, 713, 1137, 1166
GlaI GCGC 2 cut(s) 1282, 1284
GluI GCNGC 3 cut(s) 287, 429, 1149
HaeIII GGCC 3 cut(s) 224, 364, 542
HapII CCGG 7 cut(s) 221, 290, 353, 361, 380, 483, 882
HgaI GACGC 2 cut(s) 223, 705
HhaI GCGC 2 cut(s) 1283, 1285
Hin1I GRCGYC 1 cut(s) 697
Hin1II CATG 4 cut(s) 467, 472, 957, 986
Hin6I GCGC 2 cut(s) 1281, 1283
HinP1I GCGC 2 cut(s) 1281, 1283
HincII GTYRAC 1 cut(s) 1332
HindII GTYRAC 1 cut(s) 1332
HindIII AAGCTT 1 cut(s) 1058
HinfI GANTC 8 cut(s) 437, 500, 607, 803, 893, 950, 1043, 1097
HpaII CCGG 7 cut(s) 221, 290, 353, 361, 380, 483, 882
HphI GGTGA 4 cut(s) 63, 878, 929, 1195
Hpy166II GTNNAC 3 cut(s) 333, 421, 1332
Hpy188I TCNGA 7 cut(s) 205, 447, 516, 529, 566, 682, 974
Hpy188III TCNNGA 6 cut(s) 261, 334, 611, 797, 947, 1166
Hpy8I GTNNAC 3 cut(s) 333, 421, 1332
HpyAV CCTTC 4 cut(s) 89, 108, 467, 628
HpyCH4III ACNGT 3 cut(s) 308, 656, 1291
HpyCH4V TGCA 8 cut(s) 782, 830, 1005, 1074, 1108, 1179, 1218, 1258
HpyF10VI GCNNNNNNNGC 1 cut(s) 1157
HpyF3I CTNAG 7 cut(s) 446, 502, 528, 563, 612, 1056, 1197
Hsp92I GRCGYC 1 cut(s) 697
Hsp92II CATG 4 cut(s) 467, 472, 957, 986
HspAI GCGC 2 cut(s) 1281, 1283
Kzo9I GATC 2 cut(s) 757, 1168
LmnI GCTCC 5 cut(s) 4, 38, 71, 1057, 1278
Lsp1109I GCAGC 2 cut(s) 415, 1160
LweI GCATC 2 cut(s) 655, 1061
MaeI CTAG 4 cut(s) 533, 713, 1137, 1166
MaeIII GTNAC 5 cut(s) 235, 308, 656, 701, 730
MalI GATC 2 cut(s) 759, 1170
MboI GATC 2 cut(s) 757, 1168
MboII GAAGA 9 cut(s) 74, 93, 174, 177, 421, 465, 540, 872, 1055
MhlI GDGCHC 2 cut(s) 9, 961
MlyI GAGTC 3 cut(s) 446, 494, 1037
MmeI TCCRAC 1 cut(s) 133
MroXI GAANNNNTTC 1 cut(s) 876
MseI TTAA 5 cut(s) 692, 722, 1190, 1293, 1326
MslI CAYNNNNRTG 1 cut(s) 281
MspA1I CMGCKG 1 cut(s) 41
MspCI CTTAAG 1 cut(s) 1325
MspI CCGG 7 cut(s) 221, 290, 353, 361, 380, 483, 882
MspR9I CCNGG 6 cut(s) 354, 366, 882, 1068, 1082, 1118
Mva1269I GAATGC 2 cut(s) 510, 1007
MvaI CCWGG 4 cut(s) 366, 1068, 1082, 1118
MvnI CGCG 2 cut(s) 170, 1283
MwoI GCNNNNNNNGC 1 cut(s) 1157
NciI CCSGG 2 cut(s) 354, 882
NdeII GATC 2 cut(s) 757, 1168
NlaIII CATG 4 cut(s) 467, 472, 957, 986
NlaIV GGNNCC 7 cut(s) 34, 351, 359, 378, 455, 556, 1345
NmuCI GTSAC 3 cut(s) 235, 656, 701
NspI RCATGY 1 cut(s) 467
PauI GCGCGC 1 cut(s) 1281
PceI AGGCCT 1 cut(s) 542
PctI GAATGC 2 cut(s) 510, 1007
PdmI GAANNNNTTC 1 cut(s) 876
PfeI GAWTC 5 cut(s) 607, 803, 893, 950, 1097
PflFI GACNNNGTC 2 cut(s) 503, 699
PfoI TCCNGGA 1 cut(s) 1066
PkrI GCNGC 3 cut(s) 288, 430, 1150
PleI GAGTC 3 cut(s) 445, 494, 1037
PpsI GAGTC 3 cut(s) 445, 494, 1037
PpuMI RGGWCCY 2 cut(s) 453, 1343
Psp124BI GAGCTC 1 cut(s) 9
Psp5II RGGWCCY 2 cut(s) 453, 1343
Psp6I CCWGG 4 cut(s) 364, 1066, 1080, 1116
PspGI CCWGG 4 cut(s) 364, 1066, 1080, 1116
PspN4I GGNNCC 7 cut(s) 34, 351, 359, 378, 455, 556, 1345
PspPI GGNCC 3 cut(s) 43, 453, 1343
PspPPI RGGWCCY 2 cut(s) 453, 1343
PstI CTGCAG 1 cut(s) 832
PsyI GACNNNGTC 2 cut(s) 503, 699
PteI GCGCGC 1 cut(s) 1281
RseI CAYNNNNRTG 1 cut(s) 281
SacI GAGCTC 1 cut(s) 9
SaqAI TTAA 5 cut(s) 692, 722, 1190, 1293, 1326
SatI GCNGC 3 cut(s) 287, 429, 1149
Sau3AI GATC 2 cut(s) 757, 1168
Sau96I GGNCC 3 cut(s) 43, 453, 1343
SchI GAGTC 3 cut(s) 446, 494, 1037
ScrFI CCNGG 6 cut(s) 354, 366, 882, 1068, 1082, 1118
SduI GDGCHC 2 cut(s) 9, 961
SfaNI GCATC 2 cut(s) 655, 1061
SfcI CTRYAG 2 cut(s) 746, 828
SinI GGWCC 3 cut(s) 43, 453, 1343
SmiMI CAYNNNNRTG 1 cut(s) 281
SmlI CTYRAG 1 cut(s) 1325
SmoI CTYRAG 1 cut(s) 1325
SseBI AGGCCT 1 cut(s) 542
SsiI CCGC 5 cut(s) 41, 145, 170, 192, 287
SspI AATATT 1 cut(s) 820
SspMI CTAG 4 cut(s) 533, 713, 1137, 1166
SstI GAGCTC 1 cut(s) 9
StuI AGGCCT 1 cut(s) 542
StyD4I CCNGG 6 cut(s) 352, 364, 880, 1066, 1080, 1116
StyI CCWWGG 1 cut(s) 712
TaaI ACNGT 3 cut(s) 308, 656, 1291
TaqI TCGA 2 cut(s) 327, 896
TauI GCSGC 1 cut(s) 289
TfiI GAWTC 5 cut(s) 607, 803, 893, 950, 1097
Tru1I TTAA 5 cut(s) 692, 722, 1190, 1293, 1326
Tru9I TTAA 5 cut(s) 692, 722, 1190, 1293, 1326
TscAI CASTG 3 cut(s) 22, 661, 1260
TseFI GTSAC 3 cut(s) 235, 656, 701
TseI GCWGC 2 cut(s) 428, 1148
Tsp45I GTSAC 3 cut(s) 235, 656, 701
TspDTI ATGAA 3 cut(s) 885, 971, 1258
TspRI CASTG 3 cut(s) 22, 661, 1260
Tth111I GACNNNGTC 2 cut(s) 503, 699
Vha464I CTTAAG 1 cut(s) 1325
VpaK11BI GGWCC 3 cut(s) 43, 453, 1343
XagI CCTNNNNNAGG 1 cut(s) 1085
XapI RAATTY 4 cut(s) 521, 628, 906, 978
XbaI TCTAGA 1 cut(s) 1165
XceI RCATGY 1 cut(s) 467
XcmI CCANNNNNNNNNTGG 1 cut(s) 600
XmaJI CCTAGG 1 cut(s) 712
XmiI GTMKAC 1 cut(s) 420
XmnI GAANNNNTTC 1 cut(s) 876
XspI CTAG 4 cut(s) 533, 713, 1137, 1166
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.